SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 3NX2_MICMP / C0HJR2 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 11 hits to proteins with known functional sites (download)

C0HJR2 Micrurotoxin 2; MmTX2 from Micrurus mipartitus (Red-tailed coral snake) (see paper)
100% identity, 100% coverage: 1:64/64 of query aligns to 1:64/64 of C0HJR2

query
sites
C0HJR2

L
|
L

T
|
T

C
|
C

K
|
K

T
|
T

C
|
C

P
|
P

F
|
F

T
|
T

C
|
C

P
|
P

N
|
N

S
|
S

E
|
E

S
|
S

C
|
C

P
|
P

G
|
G

Q
|
Q

S
|
S

I
|
I

C
|
C

Y
|
Y

Q
|
Q

R
|
R

K
|
K

W
|
W

E
|
E

H
|
H

G
|
G

E
|
E

R
|
R

I
|
I

E
|
E

R
|
R

C
|
C

V
|
V

A
|
A

N
|
N

C
|
C

P
|
P

A
|
A

F
|
F

G
|
G

S
|
S

H
|
H

D
|
D

T
|
T

S
|
S

L
|
L

C
|
C

T
|
T

R
|
R

D
|
D

N
|
N

C
|
C

N
|
N

1:64 (vs. 1:64, 100% identical) modified: mature protein, Micrurotoxin 2
H33 (= H33) mutation to S: Loss of activity.

Q9YGJ0 Gamma-bungarotoxin; Long neurotoxin homolog NTL2I from Bungarus multicinctus (Many-banded krait) (see 2 papers)
47% identity, 100% coverage: 1:64/64 of query aligns to 22:88/89 of Q9YGJ0

query
sites
Q9YGJ0

L
x
M

T
x
Q

C
|
C

K
|
K

T
|
T

C
|
C

P
x
S

F
|
F

T
x
Y

T
|
T

C
|
C

P
|
P

N
|
N

S
|
S

E
|
E

S
x
T

C
|
C

P
|
P

G
x
D

G
|
G

Q
x
K

S
x
N

I
|
I

C
|
C

Y
x
V

Q
x
K

R
|
R

K
x
S

W
|
W

E
x
T

E
x
A

H
x
V

H
x
R

G
|
G

E
x
D

-
x
G

-
x
P

-
x
K

-
x
R

R
x
E

I
|
I

E
x
R

R
|
R

R
x
E

C
|
C

V
x
A

A
|
A

N
x
T

C
|
C

P
|
P

A
x
P

F

-

G
x
S

S
x
K

H
x
L

D
x
G

T
x
L

S
x
T

L
x
V

L
x
F

C
|
C

T
|
T

R
x
T

D
|
D

N
|
N

C
|
C

N
|
N

C24 (= C3) modified: Disulfide link with 45
C27 (= C6) modified: Disulfide link with 32
C32 (= C11) modified: Disulfide link with 27
C38 (= C17) modified: Disulfide link with 66
C45 (= C24) modified: Disulfide link with 24
C66 (= C41) modified: Disulfide link with 38
C70 (= C45) modified: Disulfide link with 81
C81 (= C57) modified: Disulfide link with 70
C82 (= C58) modified: Disulfide link with 87
C87 (= C63) modified: Disulfide link with 82

Sites not aligning to the query:

1:21 signal peptide
22:89 modified: mature protein, Gamma-bungarotoxin

P81783 Candoxin from Bungarus candidus (Malayan krait) (see 2 papers)
42% identity, 100% coverage: 1:64/64 of query aligns to 22:87/87 of P81783

query
sites
P81783

L
x
M

T
x
K

C
|
C

K
|
K

T
x
I

C
|
C

P
x
N

F
|
F

T
x
D

T
|
T

C
|
C

P
x
R

N
x
A

S
x
G

E
|
E

-
x
L

-
x
K

S
x
V

C
|
C

P
x
A

G
x
S

G
|
G

Q
x
E

S
x
K

I
x
Y

C
|
C

Y
x
F

Q
x
K

R
x
E

K
x
S

W
|
W

E
x
R

E
|
E

H
x
A

H
x
R

G
|
G

E
x
T

R
|
R

I
|
I

E
|
E

R
|
R

R
x
G

C
|
C

V
x
A

A
|
A

N
x
T

C
|
C

P
|
P

A
x
K

F
x
G

G
x
S

S
x
V

H
x
Y

D
x
G

T
x
L

S
x
Y

L
x
V

L
|
L

C
|
C

T
|
T

R
x
T

D
|
D

N
x
D

C
|
C

N
|
N

22:87 (vs. 1:64, 42% identical) modified: mature protein, Candoxin
C24 (= C3) modified: Disulfide link with 47
C27 (= C6) modified: Disulfide link with 32
C32 (= C11) modified: Disulfide link with 27
C40 (= C17) modified: Disulfide link with 64
C47 (= C24) modified: Disulfide link with 24
C64 (= C41) modified: Disulfide link with 40
C68 (= C45) modified: Disulfide link with 80
C80 (= C57) modified: Disulfide link with 68
C81 (= C58) modified: Disulfide link with 86
C86 (= C63) modified: Disulfide link with 81

Sites not aligning to the query:

1:21 signal peptide

P01398 Kappa-bungarotoxin; Kappa-bgt; Kappa-1-bungarotoxin; Long neurotoxin 2; Neuronal bungarotoxin; nBgt; Toxin F from Bungarus multicinctus (Many-banded krait) (see 6 papers)
45% identity, 95% coverage: 4:64/64 of query aligns to 22:86/87 of P01398

query
sites
P01398

K

R

T

T

C
|
C

P

L

F

I

T

S

T

P

C

S

P

S

N

T

S

P

E

Q

S

T

C
|
C

P

P

G

N

G

G

Q

Q

S

D

I

I

C
|
C

Y

F

Q

L

R

K

-

A

-
x
Q

-
x
C

-

D

K

K

W

F

E
x
C

E

S

H

I

H
x
R

G

G

E
x
P

R

V

I

I

E

E

R

Q

R

G

C
|
C

V

V

A

A

N

T

C
|
C

P

P

A

Q

F

F

G

R

S

S

H

N

D

Y

T

R

S

S

L

L

C
|
C

T

T

R

T

D

D

N

N

C
|
C

N

N

C24 (= C6) modified: Disulfide link with 42
C35 (= C17) modified: Disulfide link with 63
C42 (= C24) modified: Disulfide link with 24
Q47 (vs. gap) mutation to W: Little increase in inhibition of neuronal receptor and 8-fold increase in inhibition of muscle receptor.
C48 (vs. gap) modified: Disulfide link with 52
C52 (≠ E30) modified: Disulfide link with 48
R55 (≠ H33) mutation to A: Loss of inhibition of neuronal and muscle receptors.
P57 (≠ E35) mutation to A: No important change in inhibition of muscle and neuronal receptors.; mutation to K: 13-fold decrease in inhibition of neuronal receptor, and complete loss of activity towards muscle receptor.
C63 (= C41) modified: Disulfide link with 35
C67 (= C45) modified: Disulfide link with 79
C79 (= C57) modified: Disulfide link with 67
C80 (= C58) modified: Disulfide link with 85
C85 (= C63) modified: Disulfide link with 80

Sites not aligning to the query:

1:21 signal peptide

P60770 Cobrotoxin; CBT; CBTX; CTX; Atratoxin; Cobratide; Short neurotoxin 1 from Naja atra (Chinese cobra) (see 7 papers)
36% identity, 100% coverage: 1:64/64 of query aligns to 22:82/83 of P60770

query
sites
P60770

L
|
L

T
x
E

C
|
C

K
x
H

T
x
N

C
x
Q

P
x
Q

F
x
S

T
x
S

T
x
Q

C
x
T

P
|
P

N
x
T

S
x
T

E
x
T

S
x
G

C
|
C

P
x
S

G
|
G

Q
x
E

S
x
T

I
x
N

C
|
C

Y
|
Y

Q
x
K

R
x
K

K
x
R

W
|
W

E
x
R

E
x
D

H
|
H

H
x
R

G
|
G

E
x
Y

R
|
R

I
x
T

E
|
E

R
|
R

R
x
G

C
|
C

V

-

A

-

N
x
G

C
|
C

P
|
P

A
x
S

F
x
V

G

-

S
x
K

H
x
N

D
x
G

T
x
I

S
x
E

L
x
I

L
x
N

C
|
C

T
|
T

R
x
T

D
|
D

N
x
R

C
|
C

N
|
N

C24 (= C3) modified: Disulfide link with 45
C38 (= C17) modified: Disulfide link with 62
C45 (= C24) modified: Disulfide link with 24
E59 (= E38) mutation to Q: Decrease in the rate of isomerization reaction of disulfide bonds.
C62 (= C41) modified: Disulfide link with 38
C64 (= C45) modified: Disulfide link with 75
K68 (≠ S50) mutation to E: Decrease in the rate of isomerization reaction of disulfide bonds.; mutation to Q: Decrease in the rate of isomerization reaction of disulfide bonds.
C75 (= C57) modified: Disulfide link with 64
C76 (= C58) modified: Disulfide link with 81
C81 (= C63) modified: Disulfide link with 76

Sites not aligning to the query:

1:21 signal peptide
22:83 modified: mature protein, Cobrotoxin

Q9PTT0 Cobrotoxin homolog; Short neurotoxin from Naja naja (Indian cobra) (see paper)
36% identity, 100% coverage: 1:64/64 of query aligns to 22:82/83 of Q9PTT0

query
sites
Q9PTT0

L
|
L

T
x
E

C
|
C

K
x
H

T
x
N

C
x
Q

P
x
Q

F
x
S

T
x
S

T
x
Q

C
x
T

P
|
P

N
x
T

S
x
T

E
x
T

S
x
G

C
|
C

P
x
S

G
|
G

Q
x
E

S
x
T

I
x
N

C
|
C

Y
|
Y

Q
x
K

R
x
K

K
x
R

W
|
W

E
x
R

E
x
D

H
|
H

H
x
R

G
|
G

E
x
Y

R
|
R

I
x
T

E
|
E

R
|
R

R
x
G

C
|
C

V

-

A

-

N
x
G

C
|
C

P
|
P

A
x
S

F
x
V

G

-

S
x
K

H
x
N

D
x
G

T
x
I

S
x
E

L
x
I

L
x
N

C
|
C

T
|
T

R
x
T

D
|
D

N
x
R

C
|
C

N
|
N

C24 (= C3) modified: Disulfide link with 45
C38 (= C17) modified: Disulfide link with 62
C45 (= C24) modified: Disulfide link with 24
C62 (= C41) modified: Disulfide link with 38
C64 (= C45) modified: Disulfide link with 75
C75 (= C57) modified: Disulfide link with 64
C76 (= C58) modified: Disulfide link with 81
C81 (= C63) modified: Disulfide link with 76

Sites not aligning to the query:

1:21 signal peptide
22:83 modified: mature protein, Cobrotoxin homolog

1v6pA Crystal structure of cobrotoxin (see paper)
36% identity, 100% coverage: 1:64/64 of query aligns to 1:61/62 of 1v6pA

query
sites
1v6pA

L

L

T
x
E

C

C

K
x
H

T

N

C

Q

P

Q

F

S

T

S

T

Q

C

T

P

P

N

T

S

T

E

T

S

G

C

C

P

S

G

G

Q

E

S

T

I

N

C

C

Y

Y

Q

K

R

K

K

R

W

W

E

R

E

D

H

H

H

R

G

G

E

Y

R

R

I

T

E

E

R

R

R

G

C

C

V

-

A

-

N

G

C

C

P

P

A

S

F

V

G

-

S

K

H

N

D

G

T

I

S

E

L

I

L

N

C

C

T

T

R

T

D
|
D

N

R

C

C

N

N

binding copper (ii) ion: E2 (≠ T2), H4 (≠ K4), D58 (= D61)

Sites not aligning to the query:

binding copper (ii) ion: 62

A8N286 Haditoxin; Muscarinic toxin-like protein 3 homolog; MTLP-3 homolog from Ophiophagus hannah (King cobra) (Naja hannah) (see paper)
38% identity, 88% coverage: 9:64/64 of query aligns to 30:85/86 of A8N286

query
sites
A8N286

T
|
T

C
x
P

P
x
E

N
x
T

S
x
T

E
|
E

S
x
I

C
|
C

P
|
P

G
x
D

G
x
S

Q
x
G

S
x
Y

I
x
F

C
|
C

Y
|
Y

Q
x
K

R
x
S

K
x
S

W
|
W

E
x
I

E
x
D

H
x
G

H
x
R

G
x
E

E
x
G

R
|
R

I
|
I

E
|
E

R
|
R

R
x
G

C
|
C

V
x
T

A
x
F

N
x
T

C
|
C

P
|
P

A
x
E

F
x
L

G
x
T

S
x
P

H
x
N

D
x
G

T
x
K

S
x
Y

L
x
V

L
x
Y

C
|
C

T
x
R

R
|
R

D
|
D

N
x
K

C
|
C

N
|
N

C38 (= C17) modified: Disulfide link with 62
C45 (= C24) modified: Disulfide link with 24
C62 (= C41) modified: Disulfide link with 38
C66 (= C45) modified: Disulfide link with 78
C78 (= C57) modified: Disulfide link with 66
C79 (= C58) modified: Disulfide link with 84
C84 (= C63) modified: Disulfide link with 79

Sites not aligning to the query:

22:86 modified: mature protein, Haditoxin
24 modified: Disulfide link with 45

C0HJD7 Alpha-elapitoxin-Dpp2d; Alpha-EPTX-Dpp2d from Dendroaspis polylepis polylepis (Black mamba) (see paper)
37% identity, 95% coverage: 4:64/64 of query aligns to 1:64/72 of C0HJD7

query
sites
C0HJD7

K
x
R

T
|
T

C
|
C

P
x
N

F
x
K

T
|
T

T
x
F

C
x
S

P
x
D

N
x
Q

S
|
S

E
x
K

S
x
I

C
|
C

P
|
P

G
x
P

G
|
G

Q
x
E

S
x
N

I
|
I

C
|
C

Y
|
Y

Q
x
T

R
x
K

K
x
T

W
|
W

E
x
C

E
x
D

-
x
A

-
x
F

-
x
C

-
x
S

H
x
Q

H
x
R

G
|
G

E
x
K

R
|
R

I
x
V

E
|
E

R
x
L

R
x
G

C
|
C

V
x
A

A
|
A

N
x
T

C
|
C

P
|
P

A
x
K

F
x
V

G
x
K

S
x
A

H
x
G

D

-

T
x
V

S
x
E

L
x
I

L
x
K

C
|
C

T
x
S

R
x
T

D
|
D

N
|
N

C
|
C

N
|
N

C3 (= C6) modified: Disulfide link with 21
C14 (= C17) modified: Disulfide link with 42
C21 (= C24) modified: Disulfide link with 3
C27 (≠ E30) modified: Disulfide link with 31
C31 (vs. gap) modified: Disulfide link with 27
C42 (= C41) modified: Disulfide link with 14
C46 (= C45) modified: Disulfide link with 57
C57 (= C57) modified: Disulfide link with 46
C58 (= C58) modified: Disulfide link with 63
C63 (= C63) modified: Disulfide link with 58

Sites not aligning to the query:

1:72 modified: mature protein, Alpha-elapitoxin-Dpp2d
72 modified: Arginine amide

P01386 Long neurotoxin 2; Neurotoxin B from Ophiophagus hannah (King cobra) (Naja hannah) (see paper)
40% identity, 80% coverage: 14:64/64 of query aligns to 11:64/73 of P01386

query
sites
P01386

S

S

E

Q

S

T

C
|
C

P

P

G

D

G

G

Q

Q

S

D

I

I

C
|
C

Y

Y

Q

T

R

K

K

T

W

W

E
x
C

E

D

-

G

-

F

-
x
C

-

S

H

S

H

R

G

G

E

K

R

R

I

I

E

D

R

L

R

G

C
|
C

V

A

A

A

N

T

C
|
C

P

P

A

K

F

V

G

-

S

K

H

P

D

G

T

V

S

D

L

I

L

K

C
|
C

T

S

R

T

D

D

N

N

C
|
C

N

N

C14 (= C17) modified: Disulfide link with 42
C21 (= C24) modified: Disulfide link with 3
C27 (≠ E30) modified: Disulfide link with 31
C31 (vs. gap) modified: Disulfide link with 27
C42 (= C41) modified: Disulfide link with 14
C46 (= C45) modified: Disulfide link with 57
C57 (= C57) modified: Disulfide link with 46
C58 (= C58) modified: Disulfide link with 63
C63 (= C63) modified: Disulfide link with 58

Sites not aligning to the query:

3 modified: Disulfide link with 21

P82849 Cobrotoxin II; CBT II; CBT2; Short neurotoxin 1; Short neurotoxin 5 from Naja kaouthia (Monocled cobra) (Naja siamensis)
34% identity, 100% coverage: 1:64/64 of query aligns to 1:61/62 of P82849

query
sites
P82849

L
|
L

T
x
E

C
|
C

K
x
H

T
x
N

C
x
Q

P
x
Q

F
x
S

T
x
S

T
x
Q

C
x
T

P
|
P

N
x
T

S
x
T

E
x
T

S
x
G

C
|
C

P
x
S

G
|
G

Q
x
E

S
x
N

I
x
N

C
|
C

Y
|
Y

Q
x
K

R
x
K

K
x
E

W
|
W

E
x
R

E
x
D

H
x
N

H
x
R

G
|
G

E
x
Y

R
|
R

I
x
T

E
|
E

R
|
R

R
x
G

C
|
C

V

-

A

-

N
x
G

C
|
C

P
|
P

A
x
S

F
x
V

G

-

S
x
K

H
x
K

D
x
G

T
x
I

S
x
G

L
x
I

L
x
N

C
|
C

T
|
T

R
x
T

D
|
D

N
x
R

C
|
C

N
|
N

C3 (= C3) modified: Disulfide link with 24
C17 (= C17) modified: Disulfide link with 41
C24 (= C24) modified: Disulfide link with 3
C41 (= C41) modified: Disulfide link with 17
C43 (= C45) modified: Disulfide link with 54
C54 (= C57) modified: Disulfide link with 43
C55 (= C58) modified: Disulfide link with 60
C60 (= C63) modified: Disulfide link with 55

Sites not aligning to the query:

1:62 modified: mature protein, Cobrotoxin II

Query Sequence

>3NX2_MICMP / C0HJR2
LTCKTCPFTTCPNSESCPGGQSICYQRKWEEHHGERIERRCVANCPAFGSHDTSLLCCTR
DNCN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory