SitesBLAST

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
37% identity, 58% coverage: 79:191/195 of query aligns to 78:190/203 of P07464

query
sites
P07464

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N85 (≠ H88) binding in other chain; binding in other chain
D93 (vs. gap) binding
H115 (≠ R112) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ N144) binding in other chain
A160 (= A160) binding in other chain
TK 165:166 (≠ VS 165:166) binding
R180 (= R180) binding
R183 (= R183) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding
71 binding

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
37% identity, 58% coverage: 79:191/195 of query aligns to 77:189/200 of 1krrA

query
sites
1krrA

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binding acetyl coenzyme *a: N84 (≠ H88), I103 (= I102), A104 (≠ G103), P105 (≠ S104), T112 (≠ S110), H114 (≠ R112), G140 (= G143), S141 (≠ N144), N146 (≠ M149), G158 (≠ A159), A159 (= A160), A174 (= A175), P177 (= P178), R182 (= R183)

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
37% identity, 58% coverage: 79:191/195 of query aligns to 77:189/201 of 1krvA

query
sites
1krvA

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binding 4-nitrophenyl beta-D-galactopyranoside: Y82 (= Y84), N84 (≠ H88), V90 (≠ A94), D92 (vs. gap), M126 (≠ R131)
binding coenzyme a: A104 (≠ G103), S110 (≠ V108), T112 (≠ S110), W138 (= W141), G140 (= G143), S141 (≠ N144), N146 (≠ M149), A159 (= A160), P177 (= P178), R179 (= R180), R182 (= R183)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16, 25, 70

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
37% identity, 58% coverage: 79:191/195 of query aligns to 77:189/201 of 1kruA

query
sites
1kruA

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binding coenzyme a: H114 (≠ R112), W138 (= W141), G140 (= G143), S141 (≠ N144), G158 (≠ A159), A159 (= A160), P177 (= P178), R182 (= R183)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: Y82 (= Y84), Y82 (= Y84), N84 (≠ H88), V90 (≠ A94), D92 (vs. gap), L102 (= L101), H114 (≠ R112), M126 (≠ R131)

Sites not aligning to the query:

binding 1-methylethyl 1-thio-beta-D-galactopyranoside: 16, 22, 25, 62, 70

3dhoA Structure of streptogramin acetyltransferase in complex with an inhibitor
35% identity, 46% coverage: 96:185/195 of query aligns to 63:167/203 of 3dhoA

query
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3dhoA

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binding 6-bromo-N'-[(1Z)-(3,5-dichloro-2-hydroxyphenyl)methylidene]-2-methylquinoline-4-carbohydrazide: M84 (≠ F117), I139 (≠ V157), A141 (= A159), V147 (= V165), L155 (≠ V173), G157 (≠ A175), P160 (= P178), I164 (= I182)

1mrlA Crystal structure of streptogramin a acetyltransferase with dalfopristin (see paper)
35% identity, 46% coverage: 96:185/195 of query aligns to 63:167/204 of 1mrlA

query
sites
1mrlA

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binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: N81 (≠ V114), H82 (= H115), L93 (= L126), M102 (vs. gap), L108 (vs. gap)

Sites not aligning to the query:

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: 37, 39, 54, 56

1kk4A Crystal structure of vat(d) in complex with acetyl-coa (see paper)
35% identity, 46% coverage: 96:185/195 of query aligns to 63:167/205 of 1kk4A

query
sites
1kk4A

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binding acetyl coenzyme *a: I69 (= I102), G70 (= G103), K111 (vs. gap), W121 (= W141), G123 (= G143), K124 (≠ N144), A141 (= A159), A142 (= A160), V147 (= V165), K148 (≠ S166), L155 (≠ V173), G157 (≠ A175), G158 (= G176), P160 (= P178), I164 (= I182)

1khrA Crystal structure of vat(d) in complex with virginiamycin and coenzyme a (see paper)
35% identity, 46% coverage: 96:185/195 of query aligns to 63:167/206 of 1khrA

query
sites
1khrA

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binding coenzyme a: H82 (= H115), M84 (≠ F117), W121 (= W141), A141 (= A159), A142 (= A160), V147 (= V165), L155 (≠ V173), G157 (≠ A175), P160 (= P178), I164 (= I182)
binding virginiamycin m1: A80 (≠ N113), N81 (≠ V114), H82 (= H115), L93 (= L126)

Sites not aligning to the query:

binding virginiamycin m1: 37, 39, 54

P50870 Streptogramin A acetyltransferase; Virginiamycin acetyltransferase D; Vat(D); EC 2.3.1.- from Enterococcus faecium (Streptococcus faecium) (see paper)
35% identity, 46% coverage: 96:185/195 of query aligns to 63:167/209 of P50870

query
sites
P50870

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H82 (= H115) mutation to A: 105-fold decrease in activity.

4isxA The crystal structure of maltose o-acetyltransferase from clostridium difficile 630 in complex with acetyl-coa
29% identity, 56% coverage: 79:187/195 of query aligns to 77:186/186 of 4isxA

query
sites
4isxA

L

V

G

G

R

E

N

N

V

F

Y

F

I

A

G
x
N

S

Y

H

D

C

C

-

I

-

F

-

L

N

D

I

V

G

C

L

K

A

I

D

E

I

I

G

G

D

D

D

N

C

V

L

M

I

L

G
x
A

S

P

H

N

V

V

L

Q

V

I

T

Y

S

T

G

A

R

-

N

-

V

-

H

-

F

Y

D

H

D

P

V

I

E

D

T

A

P

Q

I

L

R

R

L

N

Q

S

G

G

G

I

E

E

R

Y

E

G

T

S

-

P

T

V

R

K

I

I

G

G

R

D

D

N

C

V

W
|
W

I

I

G
|
G

N
x
G

G

G

A

V

V

I

M

T

-

P

-

G

A

I

D

T

V

I

G

G

E

D

G

N

C

V

V

V

V

I

A

G

A
|
A

G

G

G

S

V

V

V

T

S

K

P

D

L

I

P

P

Y

N

S

T

V

V

A

A

A
x
V

G

G

V

N

P

P

A

C

R

R

V

V

I

I

R
x
K

K

K

R

I

G

E

E

E

binding acetyl coenzyme *a: N84 (≠ G86), A104 (≠ G103), W138 (= W141), G140 (= G143), G141 (≠ N144), A159 (= A160), V174 (≠ A175), K182 (≠ R183)

6x3cA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
28% identity, 62% coverage: 66:185/195 of query aligns to 17:166/207 of 6x3cA

query
sites
6x3cA

I
x
L

E

Q

F

F

M

I

T

K

T

P

F

T

V

I

T

T

P

N

E

E

A

N

V

I

L

L

-

V

G

G

R

E

N

Y

V

S

Y
|
Y

I

Y

G

D

S

S

H

K

C

R

N

G

I

E

G

S

L

F

A

E

D

D

-

Q

-

V

-

L

-
x
Y

-

H

-
x
Y

-

E

-

V

I

I

G

G

D

D

D

K

C

L

L

I

I

I

G

G

S

R

H

F

-

C

-

S

-

I

-
x
G

-

P

-

G

-

T

V

T

L

F

V

I

T

M

S

N

G
|
G

R

A

N
|
N

V

-

H
|
H

H

R

F

M

D

D

D

G

V

S

E

T

T

Y

P

P

I

F

R
x
H

L
|
L

-

F

-

R

Q

M

G

G

G

W

E

E

R

K

E

Y

-
x
M

-
x
P

-

S

-
x
L

-

K

-

D

-

L

-

P

-

L

-

K

-

G

T

D

T

I

R

E

I

I

G

G

R

N

D

D

C

V

W
|
W

I

I

G
|
G

N
x
R

G

D

A

V

V

T

V

I

M
|
M

-

P

-

G

A

V

D

K

V

I

G

G

E

D

G

G

C

A

V

I

A
|
A

G

E

G

A

V

V

V

T

S

K

P

N

L

V

P

A

P

P

Y

Y

S

S

V

I

A

V

A

G

G

G

V

N

P

P

A

L

R

K

V

F

I

I

R

R

K

K

R

R

binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: L17 (≠ I66), Y36 (= Y84), Y53 (vs. gap), G78 (= G111), N80 (= N113), H81 (= H115), H91 (≠ R125), L92 (= L126), M101 (vs. gap), P102 (vs. gap), L104 (vs. gap)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: Y51 (vs. gap), G69 (vs. gap), N80 (= N113), H81 (= H115), W120 (= W141), G122 (= G143), R123 (≠ N144), M128 (= M149), A140 (= A159), A141 (= A160)

4hurA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with acetyl coenzyme a (see paper)
28% identity, 62% coverage: 66:185/195 of query aligns to 17:166/211 of 4hurA

query
sites
4hurA

I

L

E

Q

F

F

M

I

T

K

T

P

F

T

V

I

T

T

P

N

E

E

A

N

V

I

L

L

-

V

G

G

R

E

N

Y

V

S

Y

Y

I

Y

G

D

S

S

H

K

C

R

N

G

I

E

G

S

L

F

A

E

D

D

-

Q

-

V

-

L

-
x
Y

-

H

-

Y

-

E

-

V

I

I

G

G

D

D

D

K

C

L

L

I

I

I

G

G

S

R

H

F

-

C

-
x
S

-
x
I

-
x
G

-

P

-

G

-

T

V

T

L

F

V

I

T

M

S

N

G

G

R
x
A

N
|
N

V

-

H

H

H

R

F

M

D

D

D

G

V

S

E

T

T

Y

P

P

I

F

R

H

L

L

-

F

-

R

Q

M

G

G

G

W

E

E

R

K

E

Y

-

M

-

P

-

S

-

L

-

K

-

D

-

L

-

P

-

L

-
x
K

-

G

T

D

T

I

R

E

I

I

G

G

R

N

D

D

C

V

W
|
W

I
|
I

G
|
G

N
x
R

G

D

A

V

V

T

V

I

M
|
M

-

P

-

G

A

V

D

K

V

I

G

G

E

D

G

G

C

A

V

I

A
|
A

G

E

G

A

V

V

V
x
T

S

K

P

N

L

V

P

A

P

P

Y

Y

S

S

V

I

A

V

A
x
G

G

G

V

N

P
|
P

A

L

R

K

V

F

I
|
I

R
|
R

K

K

R

R

binding acetyl coenzyme *a: Y51 (vs. gap), S67 (vs. gap), I68 (vs. gap), G69 (vs. gap), A79 (≠ R112), N80 (= N113), K110 (vs. gap), W120 (= W141), I121 (= I142), G122 (= G143), R123 (≠ N144), M128 (= M149), A140 (= A159), A141 (= A160), T146 (≠ V165), G156 (≠ A175), P159 (= P178), I163 (= I182), R164 (= R183)

6x3jA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f0224 (46) (see paper)
28% identity, 62% coverage: 66:185/195 of query aligns to 17:166/206 of 6x3jA

query
sites
6x3jA

I

L

E

Q

F

F

M

I

T

K

T

P

F

T

V

I

T

T

P

N

E

E

A

N

V

I

L

L

-

V

G

G

R

E

N

Y

V

S

Y

Y

I

Y

G

D

S

S

H

K

C

R

N

G

I

E

G

S

L

F

A

E

D

D

-

Q

-

V

-

L

-
x
Y

-

H

-
x
Y

-

E

-
x
V

I

I

G

G

D

D

D

K

C

L

L

I

I

I

G

G

S

R

H

F

-

C

-

S

-

I

-
x
G

-

P

-

G

-

T

V

T

L

F

V

I

T

M

S

N

G

G

R
x
A

N

N

V

-

H
|
H

H

R

F

M

D

D

D

G

V

S

E

T

T

Y

P

P

I

F

R
x
H

L
|
L

-

F

-

R

Q

M

G

G

G

W

E

E

R

K

E

Y

-
x
M

-
x
P

-

S

-

L

-

K

-

D

-
x
L

-

P

-

L

-

K

-

G

T

D

T

I

R

E

I

I

G

G

R

N

D

D

C

V

W
|
W

I

I

G
|
G

N

R

G

D

A

V

V

T

V

I

M

M

-

P

-

G

A

V

D

K

V

I

G

G

E

D

G

G

C

A

V

I

A

A

G

E

G

A

V

V

V

T

S

K

P

N

L

V

P

A

P

P

Y

Y

S

S

V

I

A

V

A

G

G

G

V

N

P

P

A

L

R

K

V

F

I

I

R

R

K

K

R

R

binding (2R)-2-[(3S,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-4,12-dimethyl-1,7,22-trioxo-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-3-yl]propyl isoquinolin-3-ylcarbamate: Y51 (vs. gap), Y53 (vs. gap), V55 (vs. gap), H91 (≠ R125), L92 (= L126), M101 (vs. gap), P102 (vs. gap), L107 (vs. gap)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (vs. gap), A79 (≠ R112), H81 (= H115), W120 (= W141), G122 (= G143)

4husA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with virginiamycin m1 (see paper)
28% identity, 62% coverage: 66:185/195 of query aligns to 17:166/212 of 4husA

query
sites
4husA

I
x
L

E

Q

F

F

M

I

T

K

T

P

F

T

V

I

T

T

P

N

E

E

A

N

V

I

L

L

-

V

G

G

R

E

N

Y

V

S

Y
|
Y

I

Y

G
x
D

S

S

H

K

C

R

N

G

I

E

G

S

L

F

A

E

D

D

-

Q

-

V

-

L

-

Y

-

H

-

Y

-

E

-

V

I

I

G

G

D

D

D

K

C

L

L

I

I

I

G

G

S

R

H

F

-

C

-

S

-

I

-

G

-

P

-

G

-

T

V

T

L

F

V

I

T

M

S

N

G

G

R

A

N

N

V

-

H

H

H

R

F

M

D

D

D

G

V

S

E

T

T

Y

P

P

I

F

R

H

L

L

-

F

-

R

Q

M

G

G

G

W

E

E

R

K

E

Y

-

M

-

P

-

S

-

L

-

K

-

D

-

L

-

P

-

L

-

K

-

G

T

D

T

I

R

E

I

I

G

G

R

N

D

D

C

V

W

W

I

I

G

G

N

R

G

D

A

V

V

T

V

I

M

M

-

P

-

G

A

V

D

K

V

I

G

G

E

D

G

G

C

A

V

I

A

A

G

E

G

A

V

V

V

T

S

K

P

N

L

V

P

A

P

P

Y

Y

S

S

V

I

A

V

A

G

G

G

V

N

P

P

A

L

R

K

V

F

I

I

R

R

K

K

R

R

binding virginiamycin m1: L17 (≠ I66), Y36 (= Y84), D38 (≠ G86)

6x3cE Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
28% identity, 62% coverage: 66:185/195 of query aligns to 17:166/203 of 6x3cE

query
sites
6x3cE

I

L

E

Q

F

F

M

I

T

K

T

P

F

T

V

I

T

T

P

N

E

E

A

N

V

I

L

L

-

V

G

G

R

E

N

Y

V

S

Y

Y

I

Y

G

D

S

S

H

K

C

R

N

G

I

E

G

S

L

F

A

E

D

D

-

Q

-

V

-

L

-
x
Y

-

H

-
x
Y

-

E

-

V

I

I

G

G

D

D

D

K

C

L

L

I

I

I

G

G

S

R

H

F

-

C

-

S

-

I

-
x
G

-

P

-

G

-

T

V

T

L

F

V

I

T

M

S

N

G
|
G

R

A

N
|
N

V

-

H
|
H

H

R

F
x
M

D

D

D

G

V

S

E

T

T

Y

P

P

I

F

R
x
H

L
|
L

-

F

-

R

Q

M

G

G

G

W

E

E

R

K

E

Y

-
x
M

-
x
P

-

S

-

L

-

K

-

D

-

L

-

P

-

L

-

K

-

G

T

D

T

I

R

E

I

I

G

G

R

N

D

D

C

V

W
|
W

I

I

G
|
G

N

R

G

D

A

V

V

T

V

I

M

M

-

P

-

G

A

V

D

K

V

I

G

G

E

D

G

G

C

A

V

I

A
|
A

G

E

G

A

V

V

V

T

S

K

P

N

L

V

P

A

P

P

Y

Y

S

S

V

I

A

V

A

G

G

G

V
x
N

P
|
P

A

L

R

K

V

F

I

I

R

R

K

K

R

R

binding magnesium ion: N158 (≠ V177), P159 (= P178)
binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: Y53 (vs. gap), G78 (= G111), N80 (= N113), H81 (= H115), H91 (≠ R125), L92 (= L126), M101 (vs. gap), P102 (vs. gap)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: Y51 (vs. gap), G69 (vs. gap), H81 (= H115), M83 (≠ F117), W120 (= W141), G122 (= G143), A140 (= A159), A141 (= A160)

7uukC Crystal structure of aminoglycoside resistance enzyme apma, complex with tobramycin (see paper)
39% identity, 28% coverage: 131:185/195 of query aligns to 176:234/276 of 7uukC

query
sites
7uukC

R

K

E

E

T

P

T

M

R

T

I

I

G

G

R

S

D

D

C

V

W

Y

I

I

G

G

N

A

G

H

A

A

V

F

V

I

M

N

A

A

D

S

-

T

-

V

-

T

-

S

V

I

G

G

E

D

G

G

C

A

V

I

A

G

A

S

G

G

G

A

V

V

V

L

S

E

P

N

L

V

P

P

Y

F

S

A

V

V

A

V

G

G

V

V

P

P

A

A

R

R

V

I

I

K

R

R

K

Y

R

R

Sites not aligning to the query:

binding tobramycin: 83, 87, 104, 105, 115, 137, 146, 147

7uunA Crystal structure of aminoglycoside resistance enzyme apma, complex with neomycin (see paper)
39% identity, 28% coverage: 131:185/195 of query aligns to 175:233/273 of 7uunA

query
sites
7uunA

R

K

E

E

T

P

T

M

R

T

I

I

G

G

R

S

D

D

C

V

W

Y

I

I

G

G

N

A

G

H

A

A

V

F

V

I

M

N

A

A

D

S

-

T

-

V

-

T

-

S

V

I

G

G

E

D

G

G

C

A

V

I

A

G

A

S

G

G

G

A

V

V

V

L

S

E

P

N

L

V

P

P

Y

F

S

A

V

V

A

V

G

G

V

V

P

P

A

A

R

R

V

I

I

K

R

R

K

Y

R

R

Sites not aligning to the query:

binding neomycin: 113, 136, 145, 146

7uujA Crystal structure of aminoglycoside resistance enzyme apma, complex with gentamicin
39% identity, 28% coverage: 131:185/195 of query aligns to 174:232/272 of 7uujA

query
sites
7uujA

R

K

E

E

T

P

T

M

R

T

I

I

G

G

R

S

D

D

C

V

W

Y

I

I

G

G

N

A

G

H

A

A

V

F

V

I

M

N

A

A

D

S

-

T

-

V

-

T

-

S

V

I

G

G

E

D

G

G

C

A

V

I

A

G

A

S

G

G

G

A

V

V

V

L

S

E

P

N

L

V

P

P

Y

F

S

A

V

V

A

V

G

G

V

V

P

P

A

A

R

R

V

I

I

K

R

R

K

Y

R

R

Sites not aligning to the query:

binding (2r,3r,4r,5r)-2-((1s,2s,3r,4s,6r)-4,6-diamino-3-((2r,3r,6s)-3-amino-6-(aminomethyl)-tetrahydro-2h-pyran-2-yloxy)-2-hydroxycyclohexyloxy)-5-methyl-4-(methylamino)-tetrahydro-2h-pyran-3,5-diol: 55, 85, 102, 135, 144, 145

7jm2A Crystal structure of aminoglycoside resistance enzyme apma, complex with apramycin
39% identity, 28% coverage: 131:185/195 of query aligns to 174:232/272 of 7jm2A

query
sites
7jm2A

R

K

E

E

T

P

T

M

R

T

I

I

G

G

R

S

D

D

C

V

W

Y

I

I

G

G

N

A

G

H

A

A

V

F

V

I

M

N

A

A

D

S

-

T

-

V

-

T

-

S

V

I

G

G

E

D

G

G

C

A

V

I

A

G

A

S

G

G

G

A

V

V

V

L

S

E

P

N

L

V

P

P

Y

F

S

A

V

V

A

V

G

G

V

V

P

P

A

A

R

R

V

I

I

K

R

R

K

Y

R

R

Sites not aligning to the query:

binding apramycin: 85, 102, 135, 144, 145, 147, 170, 172

Query Sequence

>408375 MicrobesOnline__882:408375
MARGVALLAAVPFYLLHRVESLVLGRERSFMGLSQLLALVPGIYGVWLRAAFYRLALRSC
PQSCAIEFMTTFVTPEAVLGRNVYIGSHCNIGLADIGDDCLIGSHVLVTSGRNVHHFDDV
ETPIRLQGGERETTRIGRDCWIGNGAVVMADVGEGCVVAAGGVVVSPLPPYSVAAGVPAR
VIRKRGEPRDEGGTP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory