SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 5207515 FitnessBrowser__PV4:5207515 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 9 hits to proteins with known functional sites (download)

P54582 Glycine betaine transporter BetP; Glycine betaine permease from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see 6 papers)
40% identity, 87% coverage: 67:521/523 of query aligns to 95:547/595 of P54582

query
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P54582

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W101 (= W73) mutation to A: Mainly monomeric, shows a decrease in activity and cannot be activated in response to increased osmolality; when associated with A-351.
E135 (≠ D107) mutation to A: Strongly decreased betaine transport.
G149 (= G121) mutation to A: Decreases betaine transport. No effect on activation by increased osmolality.
M150 (= M122) mutation to F: No effect on activation by increased osmolality; when associated with A-152.
G151 (= G123) mutation to A: Nearly abolishes betaine transport.
I152 (= I124) mutation to A: No effect on activation by increased osmolality; when associated with F-150.
IG 152:153 (= IG 124:125) binding
G153 (= G125) mutation to A: Decreases betaine transport and alters activation at higher osmolality.; mutation to D: Changes substrate specificity, giving rise to proton-coupled choline transport. Decreases sodium-dependent betaine transport.
F156 (= F128) mutation to A: Decreases betaine transport, but has no major effect on affinity for glycine betaine.
W189 (= W167) mutation to C: Mildly decreased betaine transport.
W194 (= W172) mutation to L: Strongly decreased betaine transport.
Y197 (= Y175) mutation to L: Nearly abolishes betaine transport.
R210 (≠ N188) mutation to A: Nearly abolishes betaine transport.
S253 (= S230) binding
G301 (= G277) mutation to L: Strongly decreased betaine transport.
N309 (= N285) mutation to A: Decreases affinity for sodium ions.
T351 (≠ H326) mutation to A: Mainly trimeric, but shows reduced activity at high osmolalities. Mainly monomeric, shows a decrease in activity and cannot be activated in response to increased osmolality; when associated with A-101.
W362 (= W333) mutation to C: Strongly decreased betaine transport.
W366 (= W337) mutation to C: No effect on betaine transport.
F369 (= F340) mutation to G: Decreases affinity for glycine betaine. Decreases betaine transport.
W371 (= W342) mutation to L: No effect on betaine transport.
W373 (= W344) mutation to A: Strongly decreases affinity for glycine betaine and betaine transport.
WWISW 373:377 (= WWISW 344:348) binding
W374 (= W345) mutation to A: Strongly decreases betaine transport, but has no major effect on affinity for glycine betaine.; mutation to L: No effect on betaine transport.
W377 (= W348) mutation to A: Abolishes betaine transport.; mutation to L: Nearly abolishes betaine transport.
F380 (= F351) mutation to A: Decreases betaine transport, but has no effect on affinity for glycine betaine.
F384 (= F355) mutation to A: Decreases betaine transport, but has no effect on affinity for glycine betaine.
R387 (= R358) mutation to A: Mildly decreased betaine transport.
R392 (= R363) mutation to K: Moderately decreased betaine transport.

4llhA Substrate bound outward-open state of the symporter betp (see paper)
39% identity, 87% coverage: 67:521/523 of query aligns to 39:488/524 of 4llhA

query
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binding 2-(trimethyl-lambda~5~-arsanyl)ethanol: M94 (= M122), G95 (= G123), D97 (≠ G125), W314 (= W344), W315 (= W345), W318 (= W348)
binding sodium ion: A91 (= A119), M94 (= M122), G95 (= G123), F405 (= F438), T408 (= T441), S409 (= S442)

Sites not aligning to the query:

binding 5-cyclohexyl-1-pentyl-beta-d-maltoside: 495, 499

3p03C Crystal structure of betp-g153d with choline bound (see paper)
39% identity, 87% coverage: 67:521/523 of query aligns to 39:487/508 of 3p03C

query
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I

L

L

G

N

S

L

I

L

P

P

T

G

T

S

L

I

M

G

A

N

Y

Y

V

L

E

S

N

N

I

F

P

Q

L

M

S

A

N

G

P

R

H

T

G

A

R

M

T

S

D

A

E

D

A

G

-

T

-

A

-

G

-

E

W

W

F

L

Q

G

G

S

W

W

T

T

V

I

F

F

Y

Y

W

W

A

A

W

W

I

I

S

S

W

W

S

S

P

P

F
|
F

V

V

G

G

M

M

F

F

I

L

A

A

R

R

V

I

S

S

K

R

G

G

R

R

T

S

V

I

R

R

E

E

F

F

I

I

T

L

A

G

V

V

L

L

V

V

P

P

T

A

A

G

V

V

T

S

L

T

I

V

W

W

M

F

S

S

V

I

F

F

G

G

M

T

A

A

I

I

D

-

Q

-

V

V

V

F

N

E

K

Q

V

N

G

G

E

E

-

S

L

I

G

W

A

G

K

D

G

G

L

A

T

A

D

E

V

E

P

Q

L

L

A

-

M

-

F

F

E

G

M

L

F

L

D

H

V

A

L

L

P

P

F

G

G

G

T

Q

L

I

L

M

S

G

I

I

A

A

V

M

V

I

L

L

V

L

L

G

V

T

F

F

I

I

T

T

S

S

S

A

D

D

S

S

G

A

S

S

L

T

V

V

I

M

D

G

S

T

I

M

T

S

A

Q

G

H

G

G

K

Q

I

L

D

E

A

A

P

N

V

K

P

W

Q

V

R

T

V

A

F

A

W

W

A

G

L

V

L

A

E

T

G

A

A

A

I

I

A

G

M

L

A

T

L

L

W

L

I

S

G

G

T

D

E

N

A

A

I

L

Q

S

A

N

L

L

Q

Q

A

N

G

V

A

T

I

I

S

V

T

A

A

A

L

T

P

P

F

F

T

L

V

F

I

V

L

V

L

I

L

G

M

L

C

M

V

F

S

A

L

L

L

V

K

K

G

D

M

L

R

S

T

N

E

D

binding choline ion: A92 (= A120), M94 (= M122), D97 (≠ G125), F320 (= F351)

P31553 L-carnitine/gamma-butyrobetaine antiporter from Escherichia coli (strain K12) (see 3 papers)
29% identity, 92% coverage: 40:521/523 of query aligns to 20:501/504 of P31553

query
sites
P31553

L

I

I

I

A

V

A

G

F

I

L

L

L

C

A

W

T

L

L

T

L

V

T

R

D

D

A

L

E

D

T

A

A

A

K

N

S

V

V

V

L

I

D

N

G

A

I

V

K

F

W

S

K

Y

I

V

I

T

G

N

A

V

F

W

D

G

W

W

L

A

F

F

I

E

W

W

S

Y

G

M

N

V

I

V

F

M

V

L

I

F

F

G

C

W

L

F

G

W

L

L

I

V

L

F

S

G

P

P

Y

Y

G

A

K

K

I

K

R

R

L

L

G

G

G

-

N

N

E

E

A

P

T

-

A

P

D

E

Y

F

S

S

F

T

L

A

S

S

W

W

L

I

S

F

M

M

L

M

F

F

A

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

M

L

F

F

W

W

S

G

V

S

A

I

E

E

P

I

V

Y

A
x
Y

Y

Y

F

I

T

S

G

-

W

-

Y

-

E

T

T

P

P

P

L

F

G

G

V

L

E

E

P

P

N

N

T

S

P

T

E

G

A

A

A

K

K

E

L

L

A

G

L

L

G

A

A

Y

T

S

M

L

F

F

H

H

W
|
W

G

G

L

P

H

L

P

P

W

W

A

A

I

T

Y

Y

G

S

V

F

V

L

A

S

L

V

S

A

L

F

A

A

F

Y

F

F

T

F

Y

F

N

V

K

R

G

K

M

M

P

E

L

V

S

-

I

I

R

R

-

P

-

S

S

S

I

T

F

L

Y

V

P

P

L

L

L

V

G

G

D

E

R

K

-

H

A

A

W

K

G

G

W

L

A

F

G

G

H

T

I

I

V

V

D

D

I

N

L

F

A

Y

V

L

L

V

A

A

T

L

L

I

F

F

G

A

L

M

A

G

T

T

S

S

L

L

G

G

L

L

G

A

A

T

Q

P

Q

L

A

V

A

T

S

E

G

C

I

M

H

Q

H

W

V

L

F

F

G

G

I

I

E

P

A

H

S

T

L

L

G

Q

L

L

Q

D

M

A

I

I

V

I

I

I

V

T

A

C

V

W

T

I

L

I

L

L

A

N

V

A

V

I

S

C

V

V

V

A

R

C

G

G

I

L

D

Q

G

K

G

G

V

V

K

R

V

I

I

A

S

S

N

D

I

V

N

R

M

S

I

Y

V

L

A

S

F

F

L

L

L

M

L

L

I

G

L

W

V

V

A

F

M

I

I

V

G

S

Y

G

A

A

V

S

S

F

L

I

G

M

S

N

I

Y

P

F

T

T

T
x
D

-

S

-

V

-

G

-

M

L

L

M

L

A
x
M

Y

Y

V

L

E

P

N
x
R

I

M

I

L

P

F

L

Y

S
x
T

N

D

P

P

H

I

G

A

R

K

T

G

D

G

E

F

A

P

W

-

F

-

Q

Q

G
|
G

W
|
W

T

T

V

V

F

F

Y

Y

W

W

A

A

W
|
W

I

V

S

I

W
x
Y

S
x
A

P
x
I

F
x
Q

V
x
M

G

S

M

I

F

F

I

L

A

A

R

R

V

I

S

S

K

R

G

G

R

R

T

T

V

V

R

R

E

E

F

L

I

C

T

F

A

G

V

M

L

V

L

L

V

G

P

L

T

T

A

A

V

S

T

T

L

W

I

I

W

L

M

W

S

T

V

V

F

L

G

G

G

S

-

N

-

T

-

L

M

L

A

L

I

I

D

D

Q

K

V

N

V

I

N

I

K

N

V

I

G

P

E

N

L

L

G

I

A

E

K

Q

G

-

L

-

T

Y

D

G

V

V

P

A

L

R

A

A

M

I

F

I

E

E

M

T

F

W

D

A

V

A

L

L

P

P

F

L

G

S

T

T

L

A

L

T

S

M

I

W

I

G

A

F

V

F

V

I

L

L

V

C

L

F

V

I

F

A

F

T

I

V

T

T

S

L

S

V

D

N

S

A

G

C

S

S

L

Y

V

T

I

L

D

A

S

M

I

S

T

T

A

C

G

R

G

E

K

V

I

R

D

D

A

G

P

E

V

E

P

P

Q

P

-

L

-

V

R

R

V

I

F

G

W

W

A

S

L

I

L

L

E

V

G

G

A

I

I

I

A

G

M

I

A

V

L

L

W

A

I

L

G

G

T

-

E

-

A

-

I

L

Q

K

A

P

L

I

Q

Q

A

T

G

A

A

I

I

I

S

A

T

G

A

G

L

C

P

P

F

L

T

F

V

F

I

V

L

N

L

I

L

M

M

V

C

T

V

L

S

S

L

F

L

I

K

K

G

D

M

A

R

K

T

Q

E

N

Y114 (≠ A136) binding ; mutation to L: Small decrease in transport activity.
W142 (= W167) binding
D288 (≠ T311) mutation to A: Retains 70% of transport activity. Forms mostly monomers.; mutation to R: Abolishes transport activity. Forms mostly monomers.; mutation to W: Retains 4% of transport activity. Forms mostly monomers.
M295 (≠ A314) mutation to E: Does not affect transport activity. Forms mostly monomers. Can also form small amounts of homodimers and homotrimers.
R299 (≠ N318) mutation to A: Does not affect transport activity. Forms mostly monomers. Can also form small amounts of homodimers and homotrimers. Shows a high tendency to aggregate.
T304 (≠ S323) mutation to A: Does not affect transport activity. Forms mostly monomers. Shows a high tendency to aggregate.
GW 315:316 (= GW 336:337) binding
W316 (= W337) mutation to L: Decrease in transport activity.
W323 (= W344) binding ; mutation to L: Abolishes transport activity.
WW 323:324 (= WW 344:345) binding
W324 (= W345) mutation to L: Abolishes transport activity.
Y327 (≠ W348) mutation to L: Strong decrease in transport activity.
YAIQ 327:330 (≠ WSPF 348:351) binding
Q330 (≠ F351) mutation to L: Decrease in transport activity.
M331 (≠ V352) binding

3hfxA Crystal structure of carnitine transporter (see paper)
29% identity, 90% coverage: 52:521/523 of query aligns to 21:490/493 of 3hfxA

query
sites
3hfxA

D

D

A

L

E

D

T

A

A

A

K

N

S

V

V

V

L

I

D

N

G

A

I

V

K

F

W

S

K

Y

I

V

I

T

G

N

A

V

F

W

D

G

W

W

L

A

F

F

I

E

W

W

S

Y

G

M

N

V

I

V

F

M

V

L

I

F

F

G

C

W

L

F

G

W

L

L

I

V

L

F

S

G

P

P

Y

Y

G

A

K

K

I

K

R

R

L

L

G

G

G

-

N

N

E

E

A

P

T

-

A

P

D

E

Y

F

S

S

F

T

L

A

S

S

W

W

L

I

S

F

M

M

L

M

F

F

A

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

M

L

F

F

W

W

S

G

V

S

A

I

E

E

P

I

V

Y

A
x
Y

Y

Y

F

I

T

S

G

-

W

-

Y

-

E

T

T

P

P

P

L

F

G

G

V

L

E

E

P

P

N

N

T

S

P

T

E

G

A

A

A

K

K

E

L

L

A

G

L

L

G

A

A

Y

T

S

M

L

F

F

H

H

W
|
W

G

G

L

P

H

L

P

P

W

W

A

A

I

T

Y

Y

G

S

V

F

V

L

A

S

L

V

S

A

L

F

A

A

F

Y

F

F

T

F

Y

F

N

V

K

R

G

K

M

M

P

E

L

V

S

-

I

I

R

R

-

P

-

S

S

S

I

T

F

L

Y

V

P

P

L

L

L

V

G

G

D

E

R

K

-

H

A

A

W

K

G

G

W

L

A

F

G

G

H

T

I

I

V

V

D

D

I

N

L

F

A

Y

V

L

L

V

A

A

T

L

L

I

F

F

G

A

L

M

A

G

T

T

S

S

L

L

G

G

L

L

G

A

A

T

Q

P

Q

L

A

V

A

T

S

E

G

C

I

M

H

Q

H

W

V

L

F

F

G

G

I

I

E

P

A

H

S

T

L

L

G

Q

L

L

Q

D

M

A

I

I

V

I

I

I

V

T

A

C

V

W

T

I

L

I

L

L

A

N

V

A

V

I

S

C

V

V

V

A

R

C

G

G

I

L

D

Q

G

K

G

G

V

V

K

R

V

I

I

A

S

S

N

D

I

V

N

R

M

S

I

Y

V

L

A

S

F

F

L

L

L

M

L

L

I

G

L

W

V

V

A

F

M

I

I

V

G

S

Y

G

A

A

V

S

S

F

L

I

G

M

S

N

I

Y

P

F

T

T

T

D

-

S

-

V

-

G

-

M

L

L

M

L

A

M

Y

Y

V

L

E

P

N

R

I

M

I

L

P

F

L

Y

S

T

N

D

P

P

H

I

G

A

R

K

T

G

D

G

E

F

A

P

W

-

F

-

Q

Q

G

G

W
|
W

T

T

V

V

F

F

Y

Y

W

W

A

A

W
|
W

I

V

S

I

W
x
Y

S

A

P

I

F
x
Q

V

M

G

S

M

I

F
|
F

I

L

A

A

R

R

V

I

S

S

K

R

G

G

R

R

T

T

V

V

R

R

E

E

F

L

I

C

T

F

A

G

V

M

L

V

L

L

V

G

P

L

T

T

A

A

V

S

T

T

L

W

I

I

W

L

M

W

S

T

V

V

F

L

G

G

G

S

-

N

-

T

-

L

M

L

A

L

I

I

D

D

Q

K

V

N

V

I

N

I

K

N

V

I

G

P

E

N

L

L

G

I

A

E

K

Q

G

-

L

-

T

Y

D

G

V

V

P

A

L

R

A

A

M

I

F

I

E

E

M

T

F

W

D

A

V

A

L

L

P

P

F

L

G

S

T

T

L

A

L

T

S

M

I

W

I

G

A

F

V

F

V

I

L

L

V

C

L

F

V

I

F

A

F

T

I

V

T

T

S

L

S

V

D

N

S

A

G

C

S

S

L

Y

V

T

I

L

D

A

S

M

I

S

T

T

A

C

G

R

G

E

K

V

I

R

D

D

A

G

P

E

V

E

P

P

Q

P

-

L

-

V

R

R

V

I

F

G

W

W

A

S

L

I

L

L

E

V

G

G

A

I

I

I

A

G

M

I

A

V

L

L

W

A

I

L

G

G

T

-

E

-

A

-

I

L

Q

K

A

P

L

I

Q

Q

A

T

G

A

A

I

I

I

S

A

T

G

A

G

L

C

P

P

F

L

T

F

V

F

I

V

L

N

L

I

L

M

M

V

C

T

V

L

S

S

L

F

L

I

K

K

G

D

M

A

R

K

T

Q

E

N

binding carnitine: Y103 (≠ A136), W131 (= W167), W305 (= W337), W312 (= W344), W313 (= W345), Y316 (≠ W348), Y316 (≠ W348), Q319 (≠ F351), F323 (= F355)

2wsxA Crystal structure of carnitine transporter from escherichia coli (see paper)
29% identity, 90% coverage: 52:521/523 of query aligns to 25:494/496 of 2wsxA

query
sites
2wsxA

D

D

A

L

E

D

T

A

A

A

K

N

S

V

V

V

L

I

D

N

G

A

I

V

K

F

W

S

K

Y

I

V

I

T

G

N

A

V

F

W

D

G

W

W

L

A

F

F

I

E

W

W

S

Y

G

M

N

V

I

V

F

M

V

L

I

F

F

G

C

W

L

F

G

W

L

L

I

V

L

F

S

G

P

P

Y

Y

G

A

K

K

I

K

R

R

L

L

G

G

G

-

N

-

E

E

A

E

T

P

A

P

D

E

Y

F

S

S

F

T

L

A

S

S

W

W

L

I

S

F

M

M

L

M

F

F

A

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

M

L

F

F

W

W

S

G

V

S

A

I

E

E

P

I

V

Y

A

Y

Y

Y

F

I

T

S

G

-

W

-

Y

-

E

T

T

P

P

P

L

F

G

G

V

L

E

E

P

P

N

N

T

S

P

T

E

G

A

A

A

K

K

E

L

L

A

G

L

L

G

A

A

Y

T

S

M

L

F

F

H

H

W
|
W

G

G

L

P

H

L

P

P

W

W

A

A

I

T

Y

Y

G

S

V

F

V

L

A

S

L

V

S

A

L

F

A

A

F

Y

F

F

T

F

Y

F

N

V

K

R

G

K

M

M

P

E

L

V

S

-

I

I

R

R

-

P

-

S

S

S

I

T

F

L

Y

V

P

P

L

L

L

V

G

G

D

E

R

K

-

H

A

A

W

K

G

G

W

L

A

F

G

G

H

T

I

I

V

V

D

D

I

N

L

F

A

Y

V

L

L

V

A

A

T

L

L

I

F

F

G

A

L

M

A

G

T

T

S

S

L

L

G

G

L

L

G

A

A

T

Q

P

Q

L

A

V

A

T

S

E

G

C

I

M

H

Q

H

W

V

L

F

F

G

G

I

I

E

P

A

H

S

T

L

L

G

Q

L

L

Q

D

M

A

I

I

V

I

I

I

V

T

A

C

V

W

T

I

L

I

L

L

A

N

V

A

V

I

S

C

V

V

V

A

R

C

G

G

I

L

D

Q

G

K

G

A

V

V

K

R

V

I

I

A

S

S

N

D

I

V

N

R

M

S

I

Y

V

L

A

S

F

F

L

L

L

M

L

L

I

G

L

W

V

V

A

F

M

I

I

V

G

S

Y

G

A

A

V

S

S

F

L

I

G

M

S

N

I

Y

P

F

T

T

T

D

-

S

-

V

-

G

-

M

L

L

M

L

A

M

Y

Y

V

L

E

P

N

R

I

M

I

L

P

F

L

Y

S

T

N

D

P

P

H

I

G

A

R

K

T

G

D
x
G

E

F

A

P

W

-

F

-

Q

Q

G
|
G

W

W

T

T

V

V

F

F

Y

Y

W

W

A

A

W
|
W

W

W

I

V

S

I

W
x
Y

S

A

P

I

F

Q

V
x
M

G

S

M

I

F

F

I

L

A

A

R

R

V

I

S

S

K

R

G

G

R

R

T

T

V

V

R

R

E

E

F

L

I

C

T

F

A

G

V

M

L

V

L

L

V

G

P

L

T

T

A

A

V

S

T

T

L

W

I

I

W

L

M

W

S

T

V

V

F

L

G

G

G

S

-

N

-

T

-

L

M

L

A

L

I

I

D

D

Q

K

V

N

V

I

N

L

K

N

V

I

G

P

E

N

L

L

G

I

A

E

K

Q

G

-

L

-

T

Y

D

G

V

V

P

A

L

R

A

A

M

I

F

I

E

E

M

T

F

W

D

A

V

A

L

L

P

P

F

L

G

S

T

T

L

A

L

T

S

M

I

W

I

G

A

F

V

F

V

I

L

L

V

C

L

F

V

I

F

A

F

T

I

V

T

T

S

L

S

V

D

N

S

A

G

C

S

S

L

Y

V

T

I

L

D

A

S

M

I

S

T

T

A

C

-

R

-

E

-

V

-

R

G

D

G

G

K

E

I

-

D

D

A

P

P

P

V

L

P

L

Q

V

R

R

V

I

F

G

W

W

A

S

L

I

L

L

E

V

G

G

A

I

I

I

A

G

M

I

A

V

L

L

W

A

I

L

G

G

T

-

E

-

A

-

I

L

Q

K

A

P

L

I

Q
|
Q

A

T

G

A

A

I

I

I

S

A

T

G

A

G

L

C

P

P

F

L

T

F

V

F

I

V

L

N

L

I

L

M

M

V

C

T

V

L

S

S

L

F

L

I

K

K

G

D

M

A

R

K

T

Q

E

N

binding 3-carboxy-n,n,n-trimethylpropan-1-aminium: W135 (= W167), G304 (≠ D330), G308 (= G336), W316 (= W344), Y320 (≠ W348), M324 (≠ V352), Q466 (= Q493)

2wswA Crystal structure of carnitine transporter from proteus mirabilis (see paper)
29% identity, 92% coverage: 40:521/523 of query aligns to 25:506/508 of 2wswA

query
sites
2wswA

L

I

I

I

A

V

A

G

F

I

L

L

L

C

A

W

T

L

L

T

L

V

T

R

D

D

A

L

E

D

T

A

A

S

K

N

S

E

V

V

L

I

D

N

G

A

I

V

K

F

W

S

K

Y

I

V

I

T

G

N

A

V

F

W

D

G

W

W

L

A

F

F

I

E

W

W

S

Y

G

M

N

V

I

I

F

M

V

F

I

G

F

G

C

W

L
x
F

G

W

L

L

I

V

L
x
F

S

G

P
x
R

Y

Y

G

A

K

K

I

K

R

R

L

L

G

G

G

-

N

-

E

D

A

E

T

K

A

P

D

E

Y

F

S

S

F

T

L

A

S

S

W

W

L

I

S

F

M

M

L

M

F

F

A

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

M

L

F

F

W

W

S

G

V

S

A

I

E

E

P

I

V

Y

A

Y

Y

Y

F

I

T

S

G

-

W

-

Y

-

E

S

T

P

P

P

L

F

G

G

V

M

E

E

P

G

N

Y

T

S

P

A

E

P

A

A

A

K

K

E

L

I

A

G

L

L

G

A

A

Y

T

S

M

L

F

F

H

H

W

W

G

G

L

P

H

L

P

P

W

W

A

A

I

T

Y

Y

G

S

V

F

V

L

A

S

L

V

S

A

L

F

A

A

F

Y

F

F

T

F

Y

F

N

V

K

R

G

K

M

M

P

E

-

V

L

I

S

R

I

P

R

S

S

S

I

T

F

L

Y

V

P

P

L

L

L

V

G

G

D

E

R

K

-

H

A

V

W

N

G

G

W

L

A

F

G

G

H

T

I

V

V

V

D

D

I

N

L

F

A

Y

V

L

L

V

A

A

T

L

L

I

F

L

G

A

L

M

A

G

T

T

S

S

L

L

G

G

L

L

G

A

A

T

Q

P

Q

L

A

V

A

T

S

E

G

C

I

I

H

Q

H

Y

V

L

F

F

G

G

I

I

E

P

A

H

S

T

L

L

G

Q

L

L

Q

D

M

A

I

I

V

I

I

I

V

S

A

C

V

W

T

I

L

L

A

N

V

A

V

I

S

C

V

V

V

A

R

F

G

G

I

L

D

Q

G

K

G

G

V

V

K

K

V

I

I

A

S

S

N

D

I

V

N

R

M

T

I

Y

V

L

A

S

F

F

L

L

L

M

L

L

I

G

L

W

V

V

A

F

M

I

I

V

G

G

Y

G

A

A

V

S

S

F

L

I

G

V

S

N

I

Y

P

F

T

T

-

D

-

S

-

V

-

G

T

T

L

L

M

L

A

M

Y

Y

V

M

E

P

N

R

I

M

I

L

P

F

L

Y

S

T

N

D

P

P

H

I

G

G

R

K

T

G

D

G

E

F

A

P

W

-

F

-

Q

Q

G

A

W

W

T

T

V

V

F

F

Y

Y

W

W

A

A

W

W

I

V

S

I

W

Y

S

A

P

I

F

Q

V

M

G

S

M

I

F

F

I

L

A

A

R

R

V

I

S

S

K

K

G

G

R

R

T

T

V

V

R

R

E

E

F

L

I

C

T

L

A

G

V

M

L

V

L

S

V

G

P

L

T

T

A

A

V

G

T

T

-

W

L

L

I

I

W

W

M

-

S

T

V

I

F

L

G

G

-

N

-

T

-

L

M

Q

A

L

I

I

D

D

Q

Q

V

N

V

I

N

L

K

N

V

I

G

P

E

Q

L

L

G

I

A

D

K

Q

G

-

L

-

T

Y

D

G

V

V

P

P

L

R

A

A

M

I

F

I

E

E

M

T

F

W

D

A

V

A

L

L

P

P

F

L

G

S

T

T

-

A

-

T

-

M

-

W

-

G

-

F

L

I

L

L

S

C

I

F

I

I

A

A

V

T

V

V

L

T

V

L

L

I

V

-

F

-

I

-

T

-

S

N

S

A

D

C

S

S

G

Y

S

T

L

L

V

A

I

M

D

S

S

T

I

C

T

R

A

S

G

M

G

K

K

E

-

G

I

A

D

D

A

P

P

P

V

L

P

L

Q

V

R

R

V

I

F

G

W

W

A

S

L

V

L

L

E

V

G

G

A

I

I

I

A

G

M

I

A

I

L

L

W

A

I

L

G

G

T

-

E

-

A

-

I

L

Q

K

A

P

L

I

Q

Q

A

T

G

A

A

I

I

I

S

A

T

G

A

G

L

C

P

P

F

L

T

F

V

F

I

V

L

N

L

I

L

M

M

V

C

T

V

L

S

S

L

F

L

I

K

K

G

D

M

A

R

K

T

V

E

H

binding 5-cyclohexyl-1-pentyl-beta-d-maltoside: F72 (≠ L87), F76 (≠ L91), R78 (≠ P93)

4m8jA Crystal structure of cait r262e bound to gamma-butyrobetaine (see paper)
29% identity, 92% coverage: 40:521/523 of query aligns to 12:493/495 of 4m8jA

query
sites
4m8jA

L

I

I

I

A

V

A

G

F

I

L

L

L

C

A

W

T

L

L

T

L

V

T

R

D

D

A

L

E

D

T

A

A

S

K

N

S

E

V

V

L

I

D

N

G

A

I

V

K

F

W

S

K

Y

I

V

I

T

G

N

A

V

F

W

D

G

W

W

L

A

F

F

I

E

W

W

S

Y

G

M

N

V

I

I

F

M

V

F

I

G

F

G

C

W

L

F

G

W

L

L

I

V

L

F

S

G

P

R

Y

Y

G

A

K

K

I

K

R

R

L

L

G

G

G

-

N

-

E

D

A

E

T

K

A

P

D

E

Y

F

S

S

F

T

L

A

S

S

W

W

L

I

S

F

M

M

L

M

F

F

A

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

M

L

F

F

W

W

S

G

V

S

A

I

E

E

P

I

V

Y

A

Y

Y

Y

F

I

T

S

G

-

W

-

Y

-

E

S

T

P

P

P

L

F

G

G

V

M

E

E

P

G

N

Y

T

S

P

A

E

P

A

A

A

K

K

E

L

I

A

G

L

L

G

A

A

Y

T

S

M

L

F

F

H

H

W

W

G

G

L

P

H

L

P

P

W
|
W

A

A

I

T

Y

Y

G

S

V

F

V

L

A

S

L

V

S

A

L

F

A

A

F

Y

F

F

T

F

Y

F

N

V

K

R

G

K

M

M

P

E

-

V

L

I

S

R

I

P

R

S

S

S

I

T

F

L

Y

V

P

P

L

L

L

V

G

G

D

E

R

K

-

H

A

V

W

N

G

G

W

L

A

F

G

G

H

T

I

V

V

V

D

D

I

N

L

F

A

Y

V

L

L

V

A

A

T

L

L

I

F

L

G

A

L

M

A

G

T

T

S

S

L

L

G

G

L

L

G

A

A

T

Q

P

Q

L

A

V

A

T

S

E

G

C

I

I

H

Q

H

Y

V

L

F

F

G

G

I

I

E

P

A

H

S

T

L

L

G

Q

L

L

Q

D

M

A

I

I

V

I

I

I

V

S

A

C

V

W

T

I

L

L

A

N

V

A

V

I

S

C

V

V

V

A

R

F

G

G

I

L

D

Q

G

K

G

G

V

V

K

K

V

I

I

A

S

S

N

D

I

V

N

E

M

T

I

Y

V

L

A

S

F

F

L

L

L

M

L

L

I

G

L

W

V

V

A

F

M

I

I

V

G

G

Y

G

A

A

V

S

S

F

L

I

G

V

S

N

I

Y

P

F

T

T

-

D

-

S

-

V

-

G

T

T

L

L

M

L

A

M

Y

Y

V

M

E

P

N

R

I

M

I

L

P

F

L

Y

S

T

N

D

P

P

H

I

G

G

R

K

T

G

D

G

E

F

A

P

W

-

F

-

Q

Q

G

A

W

W

T

T

V

V

F

F

Y

Y

W

W

A

A

W
|
W

I

V

S

I

W

Y

S

A

P

I

F

Q

V

M

G

S

M

I

F

F

I

L

A

A

R

R

V

I

S

S

K

K

G

G

R

R

T

T

V

V

R

R

E

E

F

L

I

C

T

L

A

G

V

M

L

V

L

S

V

G

P

L

T

T

A

A

V

G

T

T

-

W

L

L

I

I

W

W

M

-

S

T

V

I

F

L

G

G

-

N

-

T

-

L

M

Q

A

L

I

I

D

D

Q

Q

V

N

V

I

N

L

K

N

V

I

G

P

E

Q

L

L

G

I

A

D

K

Q

G

-

L

-

T

Y

D

G

V

V

P

P

L

R

A

A

M

I

F

I

E

E

M

T

F

W

D

A

V

A

L

L

P

P

F

L

G

S

T

T

-

A

-

T

-

M

-

W

-

G

-

F

L

I

L

L

S

C

I

F

I

I

A

A

V

T

V

V

L

T

V

L

L

I

V

-

F

-

I

-

T

-

S

N

S

A

D

C

S

S

G

Y

S

T

L

L

V

A

I

M

D

S

S

T

I

C

T

R

A

S

G

M

G

K

K

E

-

G

I

A

D

D

A

P

P

P

V

L

P

L

Q

V

R

R

V

I

F

G

W

W

A

S

L

V

L

L

E

V

G

G

A

I

I

I

A

G

M

I

A

I

L

L

W

A

I

L

G

G

T

-

E

-

A

-

I

L

Q

K

A

P

L

I

Q

Q

A

T

G

A

A

I

I

I

S

A

T

G

A

G

L

C

P

P

F

L

T

F

V

F

I

V

L

N

L

I

L

M

M

V

C

T

V

L

S

S

L

F

L

I

K

K

G

D

M

A

R

K

T

V

E

H

binding 3-carboxy-n,n,n-trimethylpropan-1-aminium: W139 (= W172), W315 (= W344), W316 (= W345)

B4EY22 L-carnitine/gamma-butyrobetaine antiporter from Proteus mirabilis (strain HI4320) (see 2 papers)
28% identity, 92% coverage: 40:521/523 of query aligns to 20:501/514 of B4EY22

query
sites
B4EY22

L

I

I

I

A

V

A

G

F

I

L

L

L

C

A

W

T

L

L

T

L

V

T

R

D

D

A

L

E

D

T

A

A

S

K

N

S

E

V

V

L

I

D

N

G

A

I

V

K

F

W

S

K

Y

I

V

I

T

G

N

A

V

F

W

D

G

W

W

L

A

F

F

I

E

W

W

S

Y

G

M

N

V

I

I

F

M

V

F

I

G

F

G

C

W

L

F

G

W

L

L

I

V

L

F

S

G

P

R

Y

Y

G

A

K

K

I

K

R

R

L

L

G

G

G

-

N

-

E

D

A

E

T

K

A

P

D

E

Y

F

S

S

F

T

L

A

S

S

W

W

L

I

S

F

M

M

L

M

F

F

A

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

M

L

F

F

W

W

S

G

V

S

A

I

E
|
E

P

I

V

Y

A

Y

Y

Y

F

I

T

S

G

-

W

-

Y

-

E

S

T

P

P

P

L

F

G

G

V

M

E

E

P

G

N

Y

T

S

P

A

E

P

A

A

A

K

K

E

L

I

A

G

L

L

G

A

A

Y

T

S

M

L

F

F

H

H

W

W

G

G

L

P

H

L

P

P

W

W

A

A

I

T

Y

Y

G

S

V

F

V

L

A

S

L

V

S

A

L

F

A

A

F

Y

F

F

T

F

Y

F

N

V

K

R

G

K

M

M

P

E

-

V

L

I

S

R

I

P

R

S

S

S

I

T

F

L

Y

T

P

P

L

L

L

V

G

G

D

E

R

K

-

H

A

V

W

N

G

G

W

L

A

F

G

G

H

T

I

V

V

V

D

D

I

N

L

F

A

Y

V

L

L

V

A

A

T

L

L

I

F

L

G

A

L

M

A

G

T

T

S

S

L

L

G

G

L

L

G

A

A

T

Q

P

Q

L

A

V

A

T

S

E

G

C

I

I

H

Q

H

Y

V

L

F

F

G

G

I

I

E

P

A

H

S

T

L

L

G

Q

L

L

Q

D

M

A

I

I

V

I

I

I

V

S

A

C

V

W

T

I

L

L

A

N

V

A

V

I

S

C

V

V

V

A

R

F

G

G

I

L

D

Q

G

K

G

G

V

V

K

K

V

I

I

A

S

S

N

D

I

V

N
x
R

M

T

I

Y

V

L

A

S

F

F

L

L

L

M

L

L

I

G

L

W

V

V

A

F

M

I

I

V

G

G

Y

G

A

A

V

S

S

F

L

I

G

V

S

N

I

Y

P

F

T

T

-

D

-

S

-

V

-

G

T

T

L

L

M

L

A

M

Y

Y

V

M

E

P

N

R

I

M

I

L

P

F

L

Y

S

T

N

D

P

P

H

I

G

G

R

K

T

G

D

G

E

F

A

P

W

-

F

-

Q

Q

G

A

W
|
W

T

T

V

V

F

F

Y

Y

W

W

A

A

W

W

I

V

S

I

W

Y

S

A

P

I

F

Q

V
x
M

G

S

M

I

F

F

I

L

A

A

R

R

V

I

S

S

K

K

G

G

R

R

T

T

V

V

R

R

E

E

F

L

I

C

T

L

A

G

V

M

L

V

L

S

V

G

P

L

T

T

A

A

V

G

T

T

-

W

L

L

I

I

W

W

M

-

S

T

V

I

F

L

G

G

-

N

-

T

-

L

M

Q

A

L

I

I

D

D

Q

Q

V

N

V

I

N

L

K

N

V

I

G

P

E

Q

L

L

G

I

A

D

K

Q

G

-

L

-

T

Y

D

G

V

V

P

P

L

R

A

A

M

I

F

I

E

E

M

T

F

W

D

A

V

A

L

L

P

P

F

L

G

S

T

T

L

A

L

T

S

M

I

W

I

G

A

F

V

F

V

I

L

L

V

C

L

F

V

I

F

A

F

T

I

V

T

T

S

L

S

I

D

N

S

A

G

C

S

S

L

Y

V

T

I

L

D

A

S

M

I

S

T

T

A

C

G

R

G

S

K

M

I

K

D

E

A

G

P

A

V

E

P

P

Q

P

-

L

-

V

R

R

V

I

F

G

W

W

A

S

L

V

L

L

E

V

G

G

A

I

I

I

A

G

M

I

A

I

L

L

W

A

I

L

G

G

T

-

E

-

A

-

I

L

Q

K

A

P

L

I

Q

Q

A

T

G

A

A

I

I

I

S

A

T

G

A

G

L

C

P

P

F

L

T

F

V

F

I

V

L

N

L

I

L

M

M

V

C

T

V

L

S

S

L

F

L

I

K

K

G

D

M

A

R

K

T

V

E

H

E111 (= E133) mutation to A: Abolishes transport activity.
R262 (≠ N285) mutation R->A,E: Strong decrease in L-carnitine transport. Mutant is Na(+)-dependent for substrate binding and transport.
W316 (= W337) mutation to A: 2.5-fold decrease in Vmax.
M331 (≠ V352) mutation to V: 10-fold decrease in Vmax.

Query Sequence

>5207515 FitnessBrowser__PV4:5207515
MTKGIDKYSIESTDYTIGQDNVQKWGFDVHNPVFGVSAGLIAAFLLATLLTDAETAKSVL
DGIKWKIIGAFDWLFIWSGNIFVIFCLGLILSPYGKIRLGGNEATADYSFLSWLSMLFAA
GMGIGLMFWSVAEPVAYFTGWYETPLGVEPNTPEAAKLALGATMFHWGLHPWAIYGVVAL
SLAFFTYNKGMPLSIRSIFYPLLGDRAWGWAGHIVDILAVLATLFGLATSLGLGAQQAAS
GIHHVFGIEASLGLQMIVIVAVTLLAVVSVVRGIDGGVKVISNINMIVAFLLLILVAMIG
YAVSLGSIPTTLMAYVENIIPLSNPHGRTDEAWFQGWTVFYWAWWISWSPFVGMFIARVS
KGRTVREFITAVLLVPTAVTLIWMSVFGGMAIDQVVNKVGELGAKGLTDVPLAMFEMFDV
LPFGTLLSIIAVVLVLVFFITSSDSGSLVIDSITAGGKIDAPVPQRVFWALLEGAIAMAL
LWIGGTEAIQALQAGAISTALPFTVILLLMCVSLLKGMRTERF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory