SitesBLAST
Comparing 5207770 Shew_0291 branched-chain amino acid aminotransferase (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
67% identity, 98% coverage: 6:305/307 of query aligns to 2:301/304 of 1iyeA
- active site: F33 (= F37), G35 (= G39), K156 (= K160), A157 (= A161), E190 (= E194), L214 (= L218)
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R60), Y92 (= Y96), Y126 (= Y130), K156 (= K160), Y161 (= Y165), E190 (= E194), G193 (= G197), E194 (≠ A198), N195 (= N199), L214 (= L218), G216 (= G220), I217 (= I221), T218 (= T222), G253 (= G257), T254 (= T258), A255 (= A259)
1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
67% identity, 98% coverage: 6:305/307 of query aligns to 2:301/304 of 1iydA
- active site: F33 (= F37), G35 (= G39), K156 (= K160), A157 (= A161), E190 (= E194), L214 (= L218)
- binding glutaric acid: Y92 (= Y96), Y126 (= Y130), A255 (= A259)
- binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K160), Y161 (= Y165), E190 (= E194), G193 (= G197), E194 (≠ A198), L214 (= L218), G216 (= G220), I217 (= I221), T218 (= T222), T254 (= T258)
1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
67% identity, 98% coverage: 6:305/307 of query aligns to 2:301/304 of 1i1mA
- active site: K156 (= K160)
- binding 4-methyl valeric acid: Y92 (= Y96), K156 (= K160), T254 (= T258), A255 (= A259)
- binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K160), Y161 (= Y165), E190 (= E194), G193 (= G197), E194 (≠ A198), L214 (= L218), G216 (= G220), I217 (= I221), T218 (= T222), G253 (= G257), T254 (= T258)
1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
67% identity, 98% coverage: 6:305/307 of query aligns to 2:301/304 of 1i1lA
- active site: K156 (= K160)
- binding 2-methylleucine: Y92 (= Y96), K156 (= K160), T254 (= T258), A255 (= A259)
- binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K160), Y161 (= Y165), E190 (= E194), G193 (= G197), G216 (= G220), I217 (= I221), T218 (= T222), T254 (= T258)
4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
46% identity, 96% coverage: 9:304/307 of query aligns to 9:303/306 of 4whxA
2ej0B Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase with pyridoxamine 5'-phosphate
46% identity, 97% coverage: 6:304/307 of query aligns to 4:301/305 of 2ej0B
- active site: F35 (= F37), G37 (= G39), K158 (= K160), E192 (= E194), L215 (= L218)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R58 (= R60), Y163 (= Y165), E192 (= E194), G195 (= G197), E196 (≠ A198), L215 (= L218), G217 (= G220), I218 (= I221), T219 (= T222), G254 (= G257), T255 (= T258)
2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
44% identity, 97% coverage: 6:304/307 of query aligns to 4:290/294 of 2ej2A
- active site: F35 (= F37), G37 (= G39), K147 (= K160), E181 (= E194), L204 (= L218)
- binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: R58 (= R60), Y94 (= Y96), Y152 (= Y165), E181 (= E194), G184 (= G197), E185 (≠ A198), L204 (= L218), G206 (= G220), I207 (= I221), T208 (= T222), T244 (= T258), A245 (= A259)
2ej3A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with gabapentin
44% identity, 97% coverage: 6:304/307 of query aligns to 4:293/297 of 2ej3A
- active site: F35 (= F37), G37 (= G39), K150 (= K160), E184 (= E194), L207 (= L218)
- binding [1-(aminomethyl)cyclohexyl]acetic acid: G187 (= G197), G246 (= G257), T247 (= T258), A248 (= A259)
- binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K160), Y155 (= Y165), E184 (= E194), G187 (= G197), L207 (= L218), G209 (= G220), I210 (= I221), T211 (= T222), G246 (= G257), T247 (= T258)
2eiyA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with 4-methylvaleric acid
44% identity, 97% coverage: 6:304/307 of query aligns to 4:293/297 of 2eiyA
- active site: F35 (= F37), G37 (= G39), K150 (= K160), E184 (= E194), L207 (= L218)
- binding 4-methyl valeric acid: F35 (= F37), Y94 (= Y96), T247 (= T258), A248 (= A259)
- binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K160), Y155 (= Y165), E184 (= E194), G187 (= G197), E188 (≠ A198), L207 (= L218), G209 (= G220), I210 (= I221), T211 (= T222), G246 (= G257), T247 (= T258)
1wrvA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase
44% identity, 97% coverage: 6:304/307 of query aligns to 4:293/297 of 1wrvA
- active site: F35 (= F37), G37 (= G39), K150 (= K160), E184 (= E194), L207 (= L218)
- binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K160), Y155 (= Y165), E184 (= E194), G187 (= G197), L207 (= L218), G209 (= G220), I210 (= I221), T211 (= T222), T247 (= T258)
6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
39% identity, 96% coverage: 9:304/307 of query aligns to 9:297/301 of 6thqB
- active site: F37 (= F37), K156 (= K160), E190 (= E194), L214 (= L218)
- binding pyridoxal-5'-phosphate: R60 (= R60), K156 (= K160), Y161 (= Y165), E190 (= E194), N195 (= N199), L214 (= L218), G216 (= G220), I217 (= I221), T218 (= T222), T254 (= T258)
- binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R60), Y97 (= Y96), K156 (= K160), Y161 (= Y165), E190 (= E194), G193 (= G197), E194 (≠ A198), N195 (= N199), G216 (= G220), I217 (= I221), T218 (= T222), G253 (= G257), T254 (= T258), A255 (= A259)
5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
40% identity, 92% coverage: 8:289/307 of query aligns to 4:278/290 of 5e25A
- active site: F33 (= F37), G35 (= G39), K151 (= K160), E184 (= E194), L207 (= L218)
- binding 2-oxoglutaric acid: Y88 (= Y96), K151 (= K160), T247 (= T258), A248 (= A259)
- binding pyridoxal-5'-phosphate: R52 (= R60), K151 (= K160), Y155 (= Y165), E184 (= E194), G187 (= G197), D188 (≠ A198), L207 (= L218), G209 (= G220), I210 (= I221), T211 (= T222), G246 (= G257), T247 (= T258)
5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
39% identity, 94% coverage: 9:296/307 of query aligns to 4:284/290 of 5mr0D
- active site: F32 (= F37), G34 (= G39), K150 (= K160), E183 (= E194), L206 (= L218)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R60), G100 (= G109), L101 (≠ I110), K150 (= K160), Y154 (= Y165), E183 (= E194), G186 (= G197), D187 (≠ A198), L206 (= L218), I209 (= I221), T210 (= T222), G245 (= G257), T246 (= T258)
6q8eA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum in pmp-form (see paper)
38% identity, 96% coverage: 9:304/307 of query aligns to 6:301/307 of 6q8eA
- active site: F34 (= F37), K156 (= K160), E190 (= E194), L214 (= L218)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R59 (= R60), K156 (= K160), Y161 (= Y165), E190 (= E194), G193 (= G197), S194 (≠ A198), C195 (≠ N199), L214 (= L218), S216 (≠ G220), I217 (= I221), T218 (= T222), G254 (= G257), T255 (= T258)
6h65C Crystal structure of the branched-chain-amino-acid aminotransferase from haliangium ochraceum
33% identity, 96% coverage: 10:304/307 of query aligns to 8:303/308 of 6h65C
- active site: F35 (= F37), K158 (= K160), E192 (= E194), L216 (= L218)
- binding pyridoxal-5'-phosphate: R60 (= R60), K158 (= K160), Y163 (= Y165), E192 (= E194), A196 (= A198), L216 (= L218), S218 (≠ G220), V219 (≠ I221), T220 (= T222), G256 (= G257), T257 (= T258)
7neaA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum (m3 mutant). (see paper)
37% identity, 96% coverage: 9:304/307 of query aligns to 6:301/309 of 7neaA
- active site: F34 (= F37), K156 (= K160), E190 (= E194), L214 (= L218)
- binding pyridoxal-5'-phosphate: R59 (= R60), K156 (= K160), Y161 (= Y165), E190 (= E194), G193 (= G197), S194 (≠ A198), L214 (= L218), S216 (≠ G220), I217 (= I221), T218 (= T222), T255 (= T258)
7p3tB Transaminase of gamma-proteobacterium (see paper)
32% identity, 93% coverage: 8:292/307 of query aligns to 5:282/299 of 7p3tB
- binding pyridoxal-5'-phosphate: R53 (= R60), K153 (= K160), R157 (≠ N164), E186 (= E194), S187 (≠ G195), A188 (= A196), A189 (≠ G197), S190 (≠ A198), G210 (= G220), I211 (= I221), T212 (= T222), T248 (= T258)
3ht5A Crystal structure of ilve a branched chain amino acid transaminase from mycobacterium tuberculosis (see paper)
31% identity, 97% coverage: 10:307/307 of query aligns to 17:334/335 of 3ht5A
- active site: K171 (= K160)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R68 (= R60), K171 (= K160), Y176 (= Y165), E207 (= E194), G210 (= G197), M211 (≠ A198), N212 (= N199), L235 (= L218), G237 (= G220), I238 (= I221), T239 (= T222), T281 (= T258)
5u3fA Structure of mycobacterium tuberculosis ilve, a branched-chain amino acid transaminase, in complex with d-cycloserine derivative (see paper)
30% identity, 97% coverage: 10:307/307 of query aligns to 16:325/326 of 5u3fA
- active site: K162 (= K160)
- binding (5-hydroxy-6-methyl-4-{[(3-oxo-2,3-dihydro-1,2-oxazol-4-yl)amino]methyl}pyridin-3-yl)methyl dihydrogen phosphate: G45 (= G39), R67 (= R60), Y110 (= Y96), K162 (= K160), Y167 (= Y165), E198 (= E194), G201 (= G197), M202 (≠ A198), N203 (= N199), L226 (= L218), G228 (= G220), I229 (= I221), T230 (= T222), T272 (= T258)
3daaA Crystallographic structure of d-amino acid aminotransferase inactivated by pyridoxyl-d-alanine (see paper)
30% identity, 92% coverage: 9:289/307 of query aligns to 4:272/277 of 3daaA
- active site: Y31 (≠ F37), V33 (≠ G39), K145 (≠ N153), E177 (= E194), L201 (= L218)
- binding n-(5'-phosphopyridoxyl)-d-alanine: Y31 (≠ F37), R50 (= R60), K145 (≠ N153), E177 (= E194), S180 (≠ G197), S181 (≠ A198), L201 (= L218), G203 (= G220), I204 (= I221), T205 (= T222), S240 (≠ G257), T241 (= T258)
Query Sequence
>5207770 Shew_0291 branched-chain amino acid aminotransferase (RefSeq)
MAAKTAELIWFNGEIMPWGDAKVHVMSHALHYGTSVFEGIRVYDTPQGPAGFRLTDHVQR
LYDSAKIYRMPVPYSFDETMQACRDIVKSNKLDAAYIRPLVFFGDVGMGITPPKDAKCDL
MVAAFPWGAYLGEGSMERGVDVAVTSWNRLAPNTIPTGAKAGGNYLSSLQISTEAKRNGF
DEGIALDVNGLVSEGAGANLFVVKKNKLYTPPATAAILLGITRDTIITLARDLGYEVVEE
PMSREFLYLADEIFMTGTAAEIVPVRSVDRIEVGAGGRGPVTQALQASFFGLFNGETQDK
WGWLELL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory