Comparing 5208198 FitnessBrowser__PV4:5208198 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P13009 Methionine synthase; 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12-dependent; MS; EC 2.1.1.13 from Escherichia coli (strain K12) (see 5 papers)
66% identity, 99% coverage: 15:1239/1240 of query aligns to 6:1225/1227 of P13009
Sites not aligning to the query:
Q99707 Methionine synthase; MS; 5-methyltetrahydrofolate--homocysteine methyltransferase; Cobalamin-dependent methionine synthase; Vitamin-B12 dependent methionine synthase; EC 2.1.1.13 from Homo sapiens (Human) (see 6 papers)
53% identity, 99% coverage: 11:1240/1240 of query aligns to 16:1264/1265 of Q99707
3ivaA Structure of the b12-dependent methionine synthase (meth) c-teminal half with adohcy bound (see paper)
62% identity, 46% coverage: 665:1239/1240 of query aligns to 3:575/576 of 3ivaA
3bulA E. Coli i690c/g743c meth c-terminal fragment (649-1227) (see paper)
62% identity, 46% coverage: 665:1239/1240 of query aligns to 3:575/577 of 3bulA
4cczA Crystal structure of human 5-methyltetrahydrofolate-homocysteine methyltransferase, the homocysteine and folate binding domains
55% identity, 51% coverage: 13:649/1240 of query aligns to 2:611/611 of 4cczA
8g3hA Structure of cobalamin-dependent methionine synthase (meth) in a resting state (see paper)
35% identity, 67% coverage: 21:854/1240 of query aligns to 9:806/841 of 8g3hA
Sites not aligning to the query:
6bdyA Crystal structure of the meth reactivation domain bound to sinefungin (see paper)
61% identity, 26% coverage: 916:1239/1240 of query aligns to 4:325/326 of 6bdyA
1mskA Methionine synthase (activation domain) (see paper)
61% identity, 26% coverage: 916:1239/1240 of query aligns to 4:325/327 of 1mskA
3k13C Structure of the pterin-binding domain metr of 5- methyltetrahydrofolate-homocysteine methyltransferase from bacteroides thetaiotaomicron
66% identity, 23% coverage: 364:652/1240 of query aligns to 4:286/287 of 3k13C
1bmtA How a protein binds b12: a 3.O angstrom x-ray structure of the b12- binding domains of methionine synthase (see paper)
66% identity, 19% coverage: 665:903/1240 of query aligns to 3:241/246 of 1bmtA
8sseA Methionine synthase, c-terminal fragment, cobalamin and reactivation domains from thermus thermophilus hb8
29% identity, 43% coverage: 670:1206/1240 of query aligns to 1:506/507 of 8sseA
3bofA Cobalamin-dependent methionine synthase (1-566) from thermotoga maritima complexed with zn2+ and homocysteine (see paper)
28% identity, 48% coverage: 7:606/1240 of query aligns to 1:538/560 of 3bofA
1q8jA Cobalamin-dependent methionine synthase (1-566) from thermotoga maritima (cd2+, hcy, methyltetrahydrofolate complex) (see paper)
28% identity, 48% coverage: 7:606/1240 of query aligns to 1:538/559 of 1q8jA
5vooA Methionine synthase folate-binding domain with methyltetrahydrofolate from thermus thermophilus hb8 (see paper)
36% identity, 20% coverage: 362:610/1240 of query aligns to 1:243/282 of 5vooA
7xcnP Crystal structure of the mttb-mttc complex at 2.7 a resolution (see paper)
33% identity, 14% coverage: 683:851/1240 of query aligns to 14:188/215 of 7xcnP
Sites not aligning to the query:
4jgiB 1.5 angstrom crystal structure of a novel cobalamin-binding protein from desulfitobacterium hafniense dcb-2 (see paper)
38% identity, 11% coverage: 712:846/1240 of query aligns to 45:174/206 of 4jgiB
Sites not aligning to the query:
2i2xB Crystal structure of methanol:cobalamin methyltransferase complex mtabc from methanosarcina barkeri (see paper)
34% identity, 14% coverage: 674:851/1240 of query aligns to 38:216/258 of 2i2xB
Sites not aligning to the query:
Q46EH4 Methanol--corrinoid protein; Methanol:corrinoid methyltransferase 1 subunit of 27 kDa; MT1 subunit 27 kDa from Methanosarcina barkeri (strain Fusaro / DSM 804) (see paper)
34% identity, 14% coverage: 674:851/1240 of query aligns to 38:216/258 of Q46EH4
Sites not aligning to the query:
3ezxA Structure of methanosarcina barkeri monomethylamine corrinoid protein
39% identity, 11% coverage: 692:823/1240 of query aligns to 26:156/212 of 3ezxA
Sites not aligning to the query:
1y80A Structure of a corrinoid (factor iiim)-binding protein from moorella thermoacetica
43% identity, 8% coverage: 757:851/1240 of query aligns to 3:99/125 of 1y80A
Sites not aligning to the query:
>5208198 FitnessBrowser__PV4:5208198
MATQSPVRNVRQINDAIRARLNKEILILDGAMGTMIQNHKLEEADFRGERFKQWPCDLKG
NNDLLVLTQPELIKQIHRDYLLAGADIIETNTFNATQVAMADYEMQELSREINLEGARLA
RTACDEVAAESGRQCYVAGVLGPTNRTCSISPDVNDPGYRNIHFDDLVEAYIESTQALIE
GGADIIMVETIFDTLNAKAALFAIETLFDQLGQRLPVMISGTITDASGRTLTGQTTEAFY
NSLRHVKPLSIGLNCALGPQELRPYVEELSKIAECYVSAHPNAGLPNEFGGYDETPEQMA
EVIGPWAEEGLLNIIGGCCGTTPDHIKAIRAAVIKHGARELPDLPVACRLSGLEPLTIDA
DSLFVNVGERTNVTGSAKFLRLIKTGEYEEALSVARDQVENGAQIIDINMDEGMLDGVEV
MQKFLNLIASEPDISRVPIMIDSSKWEVIEAGLKCIQGKGVVNSISLKEGEAKFIEQATL
VKRYGAAAIIMAFDEVGQADTMARKIEICTRAYRVLVDKVGFPPEDIIFDPNIFAIATGI
DEHDNYAVDFIEATREIKRTLPHAMISGGVSNVSFSFRGNNPVREAIHAVFLYHAIQAGM
DMGIVNAGQLAIYDDIDPDLKARVEAVVQNLPCPVQGSNNTEQLLEIAEQYRGDGAAGAK
KEDLEWRSWPVNKRLEHALVKGITEFIDQDTEEARQLASRPLDVIEGPLMDGMNVVGDLF
GSGKMFLPQVVKSARVMKKAVAYLNPYIEAEKVEGESNGKILMVTVKGDVHDIGKNIVGV
VLACNGYEVVDLGVMVPVEKIIEVAKAEKVDIIGMSGLITPSLDEMVHNVKAFHKAGLNI
PSIIGGATCSKIHTAVKIAPHAPEGAIYIADASRAVPMVAKLINKETRQATIDAAYEEYR
VMRDKRNSQAKRKQIVSLEAARENRCQQDWNAHTPFVPNQLGRQVFDDYPLEDLVDRIDW
TPFFRSWELHGHFPKILDDEVVGEEARKLYQDAKAMLKTIIDEKWLTAKAVIGLFPANTV
NYDDIEIYTDESRSKVEMTTHHLRMQIERVGNDNFCLADFVAPKDSGVADYMGGFAVTAG
HGIDEHVARFEANHDDYSAIMLKCLADRLVEAFAERMHERVRKEFWGYAADENLDNEALI
REKYKGIRPAPGYPACPDHTEKGLLWELLKPDETIDLKITESYAMFPTAAVSGWYFAHPQ
SRYFGVTNIGRDQVEDYAKRKGMSVEETERWLAPVLDYDV
Or try a new SitesBLAST search
SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory