SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 5208231 FitnessBrowser__PV4:5208231 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
45% identity, 92% coverage: 14:413/433 of query aligns to 4:420/425 of O59010

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O59010

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S65 (= S69) mutation to V: Strongly decreased chloride conductance.
R276 (≠ S274) mutation to S: Increased rate of aspartate transport; when associated with R-395.
RSS 276:278 (≠ SSS 274:276) binding L-aspartate
M311 (= M310) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
T314 (= T313) binding L-aspartate
V355 (= V354) binding L-aspartate
D394 (= D387) binding L-aspartate
M395 (= M388) mutation to R: Increased rate of aspartate transport; when associated with S-276.
R397 (= R390) mutation to A: Strongly decreased affinity for aspartate.
N401 (= N394) binding L-aspartate
D405 (= D398) mutation to N: Strongly decreased affinity for aspartate.

6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
46% identity, 92% coverage: 17:413/433 of query aligns to 5:420/426 of 6xwnB

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binding (3s)-3-(benzyloxy)-l-aspartic acid: S275 (= S275), S276 (= S276), M310 (= M310), T313 (= T313), D394 (= D387), R397 (= R390)

5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
46% identity, 92% coverage: 17:413/433 of query aligns to 5:420/427 of 5e9sA

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A

A

K

K

S

T

E

E

D

K

E

E

L

L

D

D

binding aspartic acid: R274 (≠ S274), S275 (= S275), S276 (= S276), T313 (= T313), G353 (= G353), V354 (= V354), A357 (= A357), G358 (= G358), D394 (= D387), R397 (= R390), T398 (= T391)
binding decyl-beta-d-maltopyranoside: L194 (≠ K193), G198 (≠ M197), Y202 (≠ L201)
binding sodium ion: Y87 (= Y94), T90 (= T97), S91 (≠ T98), S276 (= S276), G305 (= G305), A306 (≠ T306), T307 (= T307), N309 (= N309), N309 (= N309), M310 (= M310), D311 (= D311), S348 (= S348), I349 (= I349), G350 (= G350), T351 (= T351), N401 (= N394), V402 (= V395), D405 (= D398)

6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
46% identity, 92% coverage: 17:413/433 of query aligns to 3:418/425 of 6zgbA

query
sites
6zgbA

W

Y

M

L

S

D

V

Y

P

P

L

V

W

L

L

W

Q

K

I

I

L

L

I

W

G

G

M

L

L

V

L

L

G

G

I

A

C

V

A

F

G

G

L

L

G

I

L

A

G

G

E

H

-

F

-

G

-

Y

-

A

-

G

-

A

Q

V

A

K

V

T

L

Y

L

I

K

K

P

P

I

F

G

G

T

D

L

L

F

F

V

V

N

R

T

L

I

L

K

K

M

M

L

L

I

V

V

M

P

P

L

I

V

V

F

L

C

A

S

S

L

L

I

V

V

V

G

G

V

A

T

A

S

S

M

I

Q

S

D

P

T

-

A

A

K

R

M

L

G

G

R

R

I

V

G

G

F

V

K

K

S

I

F

V

A

V

F

Y

Y

Y

L

L

G

A

T

T

T

S

S

A

I

M

A

A

I

V

T

F

L

F

G

G

L

L

A

I

V

V

G

G

H

R

I

L

M

F

Q

N

P

V

G

G

A

A

G

N

L

V

A

N

M

L

T

G

S

S

A

G

E

T

S

G

H

K

N

A

A

I

V

E

K

A

E

Q

V

P

P

P

S

S

I

L

M

V

E

Q

T

T

L

L

I

L

N

N

I

I

V

V

P

P

T

T

N

N

P

P

I

F

A

A

A

S

L

L

A

A

S

K

G

G

Q

E

I

V

L

L

Q

P

V

V

I

I

V

F

F

F

A

A

V

I

A

I

L

L

G

G

I

I

A

A

L

I

V

T

L

Y

I

L

G

M

D

N

H

R

G

N

K

E

P

E

A

R

I

V

K

R

-

K

-

S

-

A

-

E

-

T

-

L

-

R

V

V

F

F

E

D

S

G

L

L

A

A

E

E

A

A

M

M

Y

Y

K

L

L

I

T

V

D

G

M

G

V

V

M

M

K

Q

L

Y

A

A

P

P

Y

I

G

G

V

V

F

F

G

A

L

L

M

I

A

A

W

Y

V

V

A

M

G

A

E

E

Y

Q

G

G

M

V

D

R

M

V

L

V

M

G

P

P

L

L

I

A

K

K

V

V

I

V

L

G

A

A

V

V

Y

Y

I

T

G

G

C

L

A

F

L

L

H

Q

I

I

I

V

G

I

F

T

Y

Y

S

F

L

I

V

L

L

L

T

K

F

-

V

V

A

F

K

G

L

I

N

D

P

P

M

I

Q

K

F

F

F

I

K

R

G

K

I

A

S

K

N

D

A

A

L

M

A

I

V

T

A

A

Y

F

T

V

T

T

S
x
R

S
|
S

A

S

G

G

T

T

L

L

P

P

A

V

S

T

M

M

K

R

C

V

A

A

S

E

E

E

S

E

L

M

G

G

I

V

N

D

K

K

K

G

I

I

S

F

S

S

F

F

V

T

L

L

P

P

L

L

G

G

T

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T

T

A

A

L

L

Y

Y

Q

Q

G

G

V

V

T

T

A

V

L

L

F

F

V

V

A

A

Q

N

A

A

F

I

G

G

I

H

D

P

L

L

T

T

W

L

V

G

D

Q

Y

Q

I

L

T

V

I

V

L

L

T

T

A

A

T

V

L

L

A

A

S

S

I

I

G

G

T

T

A

A

G

G

V

V

P

P

G

G

A

A

G

G

L

A

V

I

M

M

L

L

T

A

L

M

V

V

L

L

T

Q

T

S

V

V

G

G

L

L

P

D

L

L

E

T

-

P

-

G

-

S

-

P

-

V

-

A

-

L

G

A

V

Y

A

A

I

M

I

I

A

L

G

G

I

I

D

D

R

A

I

I

L

L

D
|
D

M

M

A

G

R
|
R

T
|
T

V

M

V

V

N

N

V

V

S

T

G

G

D

D

L

L

V

A

A

G

T

T

T

V

V

I

I

V

A

A

K

K

S

T

E

E

D

K

E

E

L

L

D

D

binding (2~{S},3~{S})-2-azanyl-3-[(2-nitrophenyl)methoxy]butanedioic acid: R272 (≠ S274), S273 (= S275), S274 (= S276), M308 (= M310), D392 (= D387), R395 (= R390), T396 (= T391)

6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
46% identity, 92% coverage: 17:413/433 of query aligns to 2:417/424 of 6zl4A

query
sites
6zl4A

W

Y

M
x
L

S

D

V

Y

P

P

L

V

W

L

L

W

Q

K

I

I

L

L

I

W

G

G

M

L

L

V

L

L

G

G

I

A

C

V

A

F

G

G

L

L

G

I

L

A

G

G

E

H

-

F

-

G

-

Y

-

A

-

G

-

A

Q

V

A

K

V

T

L

Y

L

I

K

K

P

P

I

F

G

G

T

D

L

L

F

F

V

V

N

R

T

L

I

L

K

K

M

M

L

L

I

V

V

M

P

P

L

I

V

V

F

L

C

A

S

S

L

L

I

V

V

V

G

G

V

A

T

A

S

S

M

I

Q

S

D

P

T

-

A

A

K

R

M

L

G

G

R

R

I

V

G

G

F

V

K

K

S

I

F

V

A

V

F

Y

Y

Y

L

L

G

A

T

T

T

S

S

A

I

M

A

A

I

V

T

F

L

F

G

G

L

L

A

I

V

V

G

G

H

R

I

L

M

F

Q

N

P

V

G

G

A

A

G

N

L

V

A

N

M

L

T

G

S

S

A

G

E

T

S

G

H

K

N

A

A

I

V

E

K

A

E

Q

V

P

P

P

S

S

I

L

M

V

E

Q

T

T

L

L

I

L

N

N

I

I

V

V

P

P

T

T

N

N

P

P

I

F

A

A

A

S

L

L

A

A

S

K

G

G

Q

E

I

V

L

L

Q

P

V

V

I

I

V

F

F

F

A

A

V

I

A

I

L

L

G

G

I

I

A

A

L

I

V

T

L

Y

I

L

G

M

D

N

H

R

G

N

K

E

P

E

A

R

I

V

K

R

-

K

-

S

-

A

-

E

-

T

-

L

-

R

V

V

F

F

E

D

S

G

L

L

A

A

E

E

A

A

M

M

Y

Y

K
x
L

L

I

T

V

D

G

M
x
G

V

V

M

M

K

Q

L

Y

A

A

P

P

Y

I

G

G

V

V

F

F

G

A

L

L

M

I

A

A

W

Y

V

V

A

M

G

A

E

E

Y

Q

G

G

M

V

D

R

M

V

L

V

M

G

P

P

L

L

I

A

K

K

V

V

I

V

L

G

A

A

V

V

Y

Y

I

T

G

G

C

L

A

F

L

L

H

Q

I

I

I

V

G

I

F

T

Y

Y

S

F

L

I

V

L

L

L

T

K

F

-

V

V

A

F

K

G

L

I

N

D

P

P

M

I

Q

K

F

F

F

I

K

R

G

K

I

A

S

K

N

D

A

A

L

M

A

I

V

T

A

A

Y

F

T

V

T

T

S
x
R

S
|
S

A

S

G

G

T

T

L

L

P

P

A

V

S

T

M

M

K
x
R

C

V

A

A

S

E

E

E

S

E

L

M

G

G

I

V

N

D

K

K

K

G

I

I

S

F

S

S

F

F

V

T

L

L

P

P

L

L

G

G

T

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T
|
T

A

A

L

L

Y

Y

Q

Q

G

G

V

V

T

T

A

V

L

L

F

F

V

V

A

A

Q

N

A

A

F

I

G

G

I

H

D

P

L

L

T

T

W

L

V

G

D

Q

Y

Q

I

L

T

V

I

V

L

L

T

T

A

A

T

V

L

L

A

A

S

S

I

I

G

G

T

T

A

A

G

G

V

V

P

P

G
|
G

A
|
A

G

G

L

A

V

I

M

M

L

L

T

A

L

M

V

V

L

L

T

Q

T

S

V

V

G

G

L

L

P

D

L

L

E

T

-

P

-

G

-

S

-

P

-

V

-

A

-

L

G

A

V

Y

A

A

I

M

I

I

A

L

G

G

I

I

D

D

R

A

I

I

L

L

D

D

M

M

A

G

R
|
R

T
|
T

V

M

V

V

N

N

V

V

S

T

G

G

D

D

L

L

V

A

A

G

T

T

T

V

V

I

I

V

A

A

K

K

S

T

E

E

D

K

E

E

L

L

D

D

binding decyl-beta-d-maltopyranoside: L3 (≠ M18), L191 (≠ K193), G195 (≠ M197), R282 (≠ K285)
binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ S274), S272 (= S275), S273 (= S276), M307 (= M310), T310 (= T313), G353 (= G356), A354 (= A357), R394 (= R390), T395 (= T391)

6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
46% identity, 91% coverage: 21:413/433 of query aligns to 2:409/416 of 6r7rA

query
sites
6r7rA

P

P

L

V

W

L

L

W

Q

K

I

I

L

L

I

W

G

G

M

L

L

V

L

L

G

G

I

A

C

V

A

F

G

G

L

L

G

I

L

A

G

G

E
x
H

-

F

-

G

-

Y

-

A

-

G

-

A

Q

V

A

K

V

T

L

Y

L

I

K

K

P

P

I

F

G

G

T

D

L

L

F

F

V

V

N

R

T

L

I

L

K

K

M

M

L

L

I

V

V

M

P

P

L

I

V

V

F

L

C

A

S

S

L

L

I

V

V

V

G

G

V

A

T

A

S

S

M

I

Q

S

D

P

T

-

A

A

K

R

M

L

G

G

R

R

I

V

G

G

F

V

K

K

S

I

F

V

A

V

F

Y

Y

Y

L

L

G

A

T

T

T

S

S

A

I

M

A

A

I

V

T

F

L

F

G

G

L

L

A

I

V

V

G

G

H

R

I

L

M

F

Q

N

P

V

G

G

A

A

G

N

L

V

A

N

M

L

T

G

S

S

A

G

E

T

S

G

H

-

N

-

A

-

V

-

K

A

E

Q

V

P

P

P

S

S

I

L

M

V

E

Q

T

T

L

L

I

L

N

N

I

I

V

V

P

P

T

T

N

N

P

P

I

F

A

A

A

S

L

L

A

A

S

K

G

G

Q

E

I

V

L

L

Q

P

V

V

I

I

V

F

F

F

A

A

V

I

A

I

L

L

G

G

I

I

A

A

L

I

V

T

L

Y

I

L

G

M

D

N

H

R

G

N

K

E

P

E

A

R

I

V

K

R

-

K

-

S

-

A

-

E

-

T

-

L

-

R

V

V

F

F

E

D

S

G

L

L

A

A

E

E

A

A

M

M

Y

Y

K

L

L

I

T

V

D

G

M

G

V

V

M

M

K

Q

L

Y

A

A

P

P

Y

I

G

G

V

V

F

F

G

A

L

L

M

I

A

A

W

Y

V

V

A

M

G

A

E

E

Y

Q

G

G

M

V

D

R

M

V

L
x
V

M

G

P

P

L

L

I
x
A

K

K

V

V

I

V

L

G

A

A

V

V

Y

Y

I

T

G

G

C

L

A

F

L

L

H

Q

I

I

I

V

G

I

F

T

Y

Y

S

F

L

I

V

L

L

L

T

K

F

-

V

V

A

F

K

G

L

I

N

D

P

P

M

I

Q

K

F

F

F

I

K

R

G

K

I

A

S

K

N

D

A

A

L

M

A

I

V

T

A

A

Y

F

T

V

T

T

S
x
R

S

S

S
|
S

A

S

G

G

T

T

L

L

P

P

A

V

S

T

M

M

K

R

C

V

A

A

S

E

E

E

S

E

L

M

G

G

I

V

N

D

K

K

K

G

I

I

S

F

S

S

F

F

V

T

L

L

P

P

L

L

G

G

T

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T
|
T

A

A

L

L

Y

Y

Q

Q

G

G

V

V

T

T

A

V

L

L

F

F

V

V

A

A

Q

N

A

A

F

I

G

G

I

H

D

P

L

L

T

T

W

L

V

G

D

Q

Y

Q

I

L

T

V

I

V

L

L

T

T

A

A

T

V

L

L

A

A

S

S

I

I

G

G

T
|
T

A

A

G
|
G

V
|
V

P

P

G

G

A

A

G
|
G

L

A

V

I

M

M

L

L

T

A

L

M

V

V

L

L

T

Q

T

S

V

V

G

G

L

L

P

D

L

L

E

T

-

P

-

G

-

S

-

P

-

V

-

A

-

L

G

A

V

Y

A

A

I

M

I

I

A

L

G

G

I

I

D

D

R

A

I

I

L

L

D
|
D

M

M

A

G

R
|
R

T
|
T

V

M

V

V

N
|
N

V

V

S

T

G

G

D

D

L

L

V

A

A

G

T

T

T

V

V

I

I

V

A

A

K

K

S

T

E

E

D

K

E

E

L

L

D

D

binding d-aspartic acid: R263 (≠ S274), S265 (= S276), M299 (= M310), T302 (= T313), T340 (= T351), G342 (= G353), V343 (= V354), G347 (= G358), D383 (= D387), R386 (= R390), T387 (= T391), N390 (= N394)
binding decyl-beta-d-maltopyranoside: H23 (≠ E42), V212 (≠ L222), A216 (≠ I226)

6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
45% identity, 92% coverage: 14:412/433 of query aligns to 4:419/419 of 6x15A

query
sites
6x15A

W
x
Y

S

R

G

K

W
x
Y

M

I

S

E

V

Y

P

P

L

V

W

L

L

Q

Q

K

I

I

L

L

I

I

G

G

M

L

L

I

L

L

G

G

I

A

C

I

A

V

G

G

L

L

G

I

L

L

G

G

E

H

-

Y

-

G

-

Y

-

A

Q

H

A

A

V

V

-

H

-

T

L

Y

L

V

K

K

P

P

I
x
F

G

G

T

D

L

L

F

F

V

V

N

R

T

L

I

L

K

C

M

M

L

L

I

V

V

M

P

P

L

I

V

V

F

F

C

A

S

S

L

L

I

V

V

V

G

G

V

A

T

A

S

S

M

I

Q

S

D
x
P

T

-

A

A

K

R

M

L

G

G

R

R

I

V

G

G

F

V

K

K

S

I

F

V

A

V

F

Y

Y
|
Y

L

L

G
x
L

T
|
T

T
x
S

S

A

I
x
F

A

A

I

V

T

T

L

L

G

G

L

I

A

I

V

M

G

A

H

R

I

L

M

F

Q

N

P

P

G

G

A

A

G

G

L

I

A

H

M

L

T

A

S

V

A

G

E

G

S

Q

H

Q

N

F

A

Q

V

P

K

H

E

Q

V

A

P

P

S

P

I
x
L

M

V

E

H

T
x
I

L

L

I

L

N

D

I

I

V

V

P

P

T

T

N

N

P

P

I

F

A

G

A

A

L

L

A

A

S

N

G

G

Q

Q

I

V

L

L

Q

P

V

T

I

I

V

F

F

F

A

A

V
x
I

A

I

L

L

G

G

I

I

A

A

L

I

V

T

L
x
Y

I

L

G

M

D

N

H

S

G

E

K

N

P

E

A

K

I

V

K

R

-

K

-

S

-

A

-

E

-

T

-

L

-

D

V

A

F

I

E

N

S

G

L

L

A

A

E

E

A

A

M

M

Y

Y

K
|
K

L

I

T

V

D

N

M
x
G

V

V

M

M

K

Q

L

Y

A

A

P

P

Y
x
I

G

G

V

V

F
|
F

G

A

L

L

M

I

A

A

W

Y

V

V

A

M

G

A

E

E

Y

Q

G

G

M

V

D

H

M

V

L

V

M

G

P

E

L

L

I

A

K

K

V

V

I

T

L

A

A

A

V

V

Y

Y

I

V

G

G

C

L

A

T

L

L

H

Q

I

I

I

L

G

L

F

V

Y

Y

S

-

L

F

V

V

L
|
L

T

L

F

K

V

I

A

Y

K

G

L

I

N

D

P

P

M

I

Q

S

F

F

F
x
I

K

K

G

H

I

A

S

K

N

D

A

A

L
x
M

A

L

V

T

A

A

Y

F

T

V

T

T

S

R

S
|
S

A

S

G

G

T

T

L

L

P

P

A

V

S

T

M

M

K

R

C

V

A

A

S

K

E

E

S

-

L

M

G

G

I

I

N

S

K

E

K

G

I

I

S

Y

S

S

F

F

V

T

L

L

P

P

L

L

G
|
G

T

A

T
|
T

I

I

N
|
N

M
|
M

D
|
D

G

G

T
|
T

A

A

L

L

Y

Y

Q

Q

G

G

V

V

T

A

A

T

L

F

F

F

V

I

A

A

Q

N

A

A

F

L

G

G

I

S

D

H

L

L

T
|
T

W
x
V

V
x
G

D

Q

Y

Q

I

L

T
|
T

I

I

I

V

L
|
L

T

T

A

A

T

V

L

L

A

A

S
|
S

I
|
I

G

G

T
|
T

A

A

G
|
G

V

V

P

P

G

G

A
|
A

G
|
G

L

A

V

I

M

M

L

L

T

C

L

M

V

V

L

L

T

H

T

S

V

V

G

G

L

L

P

P

L

L

E

T

-

D

-

P

-

N

-

V

-

A

G

A

V

Y

A

A

I

M

I

I

A

L

G

G

I

I

D

D

R

A

I

I

L

L

D
|
D

M

M

A

G

R
|
R

T
|
T

V
x
M

V

V

N
|
N

V
|
V

S

T

G

G

D
|
D

L

L

V

T

A

G

T

T

T

A

V

I

I

V

A

A

K

K

S

T

E

E

D

G

E

T

L

L

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: Y4 (≠ W14), Y7 (≠ W17), F46 (≠ I50), F46 (≠ I50), P75 (≠ D79), L91 (≠ G96), F95 (≠ I100), L130 (≠ I135), I133 (≠ T138), I159 (≠ V164), Y167 (≠ L172), K196 (= K193), G200 (≠ M197), I207 (≠ Y204), F210 (= F207), L250 (= L248), I262 (≠ F260), M269 (≠ L267), T334 (= T333), V335 (≠ W334), G336 (≠ V335), T340 (= T339), L343 (= L342), M399 (≠ V392)
binding aspartic acid: S277 (= S275), S278 (= S276), T314 (= T313), G354 (= G353), A358 (= A357), G359 (= G358), D394 (= D387), R397 (= R390), T398 (= T391)
binding sodium ion: Y89 (= Y94), T92 (= T97), S93 (≠ T98), G306 (= G305), T308 (= T307), N310 (= N309), N310 (= N309), M311 (= M310), D312 (= D311), S349 (= S348), I350 (= I349), T352 (= T351), N401 (= N394), V402 (= V395), D405 (= D398)

6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
45% identity, 91% coverage: 14:409/433 of query aligns to 1:413/413 of 6x14A

query
sites
6x14A

W

Y

S

R

G

K

W
x
Y

M

I

S

E

V

Y

P

P

L

V

W

L

L

Q

Q

K

I
|
I

L

L

I

I

G

G

M

L

L

I

L

L

G

G

I

A

C

I

A

V

G

G

L

L

G

I

L

L

G

G

E

H

-

Y

-

G

-

Y

-

A

Q

H

A

A

V

V

-

H

-

T

L

Y

L

V

K

K

P

P

I

F

G

G

T

D

L

L

F

F

V

V

N

R

T

L

I

L

K

C

M

M

L

L

I

V

V

M

P

P

L

I

V

V

F

F

C

A

S

S

L

L

I

V

V

V

G
|
G

V

A

T

A

S

S

M

I

Q

S

D

P

T

-

A

A

K

R

M

L

G

G

R

R

I

V

G

G

F

V

K

K

S

I

F
x
V

A

V

F

Y

Y

Y

L

L

G

L

T

T

T

S

S

A

I

F

A

A

I

V

T

T

L

L

G

G

L

I

A

I

V

M

G

A

H

R

I

L

M

F

Q

N

P

P

G

G

A

A

G

G

L

I

A

H

M

L

T

A

S

V

A

G

E

G

S

Q

H

Q

N

F

A

Q

V

P

K

H

E

Q

V

A

P

P

S

P

I

L

M

V

E

H

T

I

L

L

I

L

N

D

I

I

V

V

P

P

T

T

N

N

P

P

I

F

A

G

A

A

L

L

A

A

S

N

G

G

Q

Q

I

V

L

L

Q

P

V

T

I

I

V

F

F

F

A

A

V

I

A
x
I

L

L

G

G

I

I

A

A

L

I

V

T

L
x
Y

I

L

G

M

D

N

H

S

G

E

K

N

P

E

A

K

I

V

K

R

-

K

-

S

-

A

-

E

-

T

-

L

-

D

V

A

F

I

E

N

S

G

L

L

A

A

E

E

A

A

M

M

Y

Y

K
|
K

L

I

T

V

D

N

M

G

V

V

M
|
M

K

Q

L

Y

A

A

P

P

Y

I

G

G

V

V

F

F

G

A

L

L

M

I

A

A

W

Y

V

V

A

M

G

A

E

E

Y

Q

G

G

M

V

D

H

M

V

L

V

M

G

P

E

L

L

I

A

K

K

V

V

I

T

L

A

A

A

V

V

Y

Y

I

V

G

G

C

L

A

T

L

L

H

Q

I

I

I

L

G

L

F

V

Y

Y

S

-

L

F

V

V

L

L

T

L

F

K

V

I

A

Y

K

G

L

I

N

D

P

P

M

I

Q

S

F

F

F

I

K

K

G

H

I

A

S

K

N

D

A

A

L

M

A

L

V

T

A

A

Y

F

T

V

T

T

S

R

S

S

S
|
S

A

S

G

G

T

T

L

L

P

P

A

V

S

T

M

M

K

R

C

V

A

A

S

K

E

E

S

-

L

M

G

G

I

I

N

S

K

E

K

G

I

I

S

Y

S

S

F

F

V

T

L

L

P

P

L

L

G

G

T

A

T
|
T

I
|
I

N

N

M

M

D

D

G

G

T
|
T

A

A

L

L

Y

Y

Q

Q

G

G

V

V

T

A

A

T

L

F

F

F

V

I

A

A

Q

N

A

A

F

L

G

G

I

S

D

H

L

L

T

T

W

V

V

G

D

Q

Y

Q

I

L

T

T

I

I

I

V

L

L

T

T

A

A

T

V

L

L

A

A

S

S

I
|
I

G

G

T

T

A

A

G

G

V

V

P

P

G

G

A

A

G
|
G

L

A

V

I

M

M

L

L

T

C

L

M

V

V

L

L

T

H

T

S

V

V

G

G

L

L

P

P

L

L

E

T

-

D

-

P

-

N

-

V

-

A

G

A

V

Y

A

A

I

M

I

I

A
x
L

G

G

I

I

D

D

R

A

I

I

L

L

D
|
D

M

M

A

G

R
|
R

T

T

V

M

V

V

N

N

V

V

S

T

G

G

D

D

L

L

V

T

A

G

T

T

T

A

V

I

I

V

A

A

K

K

S

T

E

E

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: Y4 (≠ W17), G66 (= G73), V83 (≠ F91), I157 (≠ A165), Y164 (≠ L172), K193 (= K193), T305 (= T307), I306 (= I308), I347 (= I349)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (= I26), M199 (= M199), S275 (= S276), T311 (= T313), G356 (= G358), L384 (≠ A380), D391 (= D387), R394 (= R390)

2nwwA Crystal structure of gltph in complex with tboa (see paper)
46% identity, 90% coverage: 21:409/433 of query aligns to 2:407/407 of 2nwwA

query
sites
2nwwA

P

P

L

V

W

L

L

Q

Q

K

I

I

L

L

I

I

G

G

M

L

L

I

L

L

G

G

I

A

C

I

A

V

G

G

L

L

G

I

L

L

G

G

E

H

-

Y

-

G

-

Y

-

A

Q

H

A

A

V

V

-

H

-

T

L

Y

L

V

K

K

P

P

I

F

G

G

T

D

L

L

F

F

V

V

N

R

T

L

I

L

K

K

M

M

L

L

I

V

V

M

P

P

L

I

V

V

F

F

C

A

S

S

L

L

I

V

V

V

G

G

V

A

T

A

S

S

M

I

Q

S

D

P

T

-

A

A

K

R

M

L

G

G

R

R

I

V

G

G

F

V

K

K

S

I

F

V

A

V

F

Y

Y

Y

L

L

G

L

T

T

T

S

S

A

I

F

A

A

I

V

T

T

L

L

G

G

L

I

A

I

V

M

G

A

H

R

I

L

M

F

Q

N

P

P

G

G

A

A

G

G

L

I

A

H

M

L

T

A

S

V

A

G

E

G

S

Q

H

Q

N

F

A

Q

V

P

K

H

E

Q

V

A

P

P

S

P

I

L

M

V

E

H

T

I

L

L

I

L

N

D

I

I

V

V

P

P

T

T

N

N

P

P

I

F

A

G

A

A

L

L

A

A

S

N

G

G

Q

Q

I

V

L

L

Q

P

V

T

I

I

V

F

F

F

A

A

V

I

A

I

L

L

G

G

I

I

A

A

L

I

V

T

L

Y

I

L

G

M

D

N

H

S

G

E

K

N

P

E

A

K

I

V

K

R

-

K

-

S

-

A

-

E

-

T

-

L

-

D

V

A

F

I

E

N

S

G

L

L

A

A

E

E

A

A

M

M

Y

Y

K

K

L

I

T

V

D

N

M

G

V

V

M

M

K

Q

L

Y

A

A

P

P

Y

I

G

G

V

V

F

F

G

A

L

L

M

I

A

A

W

Y

V

V

A

M

G

A

E

E

Y

Q

G

G

M

V

D

H

M

V

L

V

M

G

P

E

L

L

I

A

K

K

V

V

I

T

L

A

A

A

V

V

Y

Y

I

V

G

G

C

L

A

T

L

L

H

Q

I

I

I

L

G

L

F

V

Y

Y

S

-

L

F

V

V

L

L

T

L

F

K

V

I

A

Y

K

G

L

I

N

D

P

P

M

I

Q

S

F

F

F

I

K

K

G

H

I

A

S

K

N

D

A

A

L

M

A

L

V

T

A

A

Y

F

T

V

T

T

S

R

S

S

S
|
S

A

S

G

G

T

T

L

L

P

P

A

V

S

T

M

M

K

R

C

V

A

A

S

K

E

E

S

-

L

M

G

G

I

I

N

S

K

E

K

G

I

I

S

Y

S

S

F

F

V

T

L

L

P

P

L

L

G

G

T

A

T

T

I

I

N

N

M

M

D

D

G

G

T
|
T

A

A

L

L

Y

Y

Q

Q

G

G

V

V

T

C

A

T

L

F

F

F

V

I

A

A

Q

N

A

A

F

L

G

G

I

S

D

H

L

L

T

T

W

V

V

G

D

Q

Y

Q

I

L

T

T

I

I

I

V

L

L

T

T

A

A

T

V

L

L

A

A

S

S

I

I

G

G

T

T

A

A

G

G

V

V

P

P

G

G

A
|
A

G
|
G

L

A

V

I

M

M

L

L

T

A

L

M

V

V

L

L

T

H

T

S

V

V

G

G

L

L

P

P

L

L

E

T

-

D

-

P

-

N

-

V

-

A

G

A

V

Y

A

A

I

M

I

I

A

L

G

G

I

I

D

D

R

A

I

I

L

L

D
|
D

M

M

A

G

R
|
R

T
|
T

V

M

V

V

N
|
N

V

V

S

T

G

G

D

D

L

L

V

T

A

G

T

T

T

A

V

I

I

V

A

A

K

K

S

T

E

E

binding (3s)-3-(benzyloxy)-l-aspartic acid: S269 (= S276), T305 (= T313), A349 (= A357), G350 (= G358), D385 (= D387), R388 (= R390), T389 (= T391), N392 (= N394)

6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
46% identity, 90% coverage: 21:409/433 of query aligns to 3:408/409 of 6bavA

query
sites
6bavA

P

P

L

V

W

L

L

Q

Q

K

I

I

L

L

I

I

G

G

M

L

L

I

L

L

G

G

I

A

C

I

A

V

G

G

L

L

G

I

L

L

G

G

E

H

Q

Y

A

G

-

Y

-

A

-

D

-

A

-

V

-

K

V

T

L

Y

L

V

K

K

P

P

I

F

G

G

T

D

L

L

F

F

V

V

N

R

T

L

I

L

K

K

M

M

L

L

I

V

V

M

P

P

L

I

V

V

F

F

C

A

S

S

L

L

I

V

V

V

G

G

V

A

T

A

S

S

M

I

Q

S

D

P

T

-

A

A

K

R

M

L

G

G

R

R

I

V

G

G

F

V

K

K

S

I

F

V

A

V

F

Y

Y

Y

L

L

G

L

T

T

T

S

S

A

I

F

A

A

I

V

T

T

L

L

G

G

L

I

A

I

V

M

G

A

H

R

I

L

M

F

Q

N

P

P

G

G

A

A

G

G

L

I

A

H

M

L

T

A

S

V

A

G

E

G

S

Q

H

Q

N

F

A

Q

V

P

K

K

E

Q

V

A

P

P

S

P

I

L

M

V

E

K

T

I

L

L

I

L

N

D

I

I

V

V

P

P

T

T

N

N

P

P

I

F

A

G

A

A

L

L

A

A

S

N

G

G

Q

Q

I

V

L

L

Q

P

V

T

I

I

V

F

F

F

A

A

V

I

A

I

L

L

G

G

I

I

A

A

L

I

V

T

L

Y

I

L

G

M

D

N

H

S

G

E

K

N

P

E

A

K

I

V

K

R

-

K

-

S

-

A

-

E

-

T

-

L

-

D

V

A

F

I

E

N

S

G

L

L

A

A

E

E

A

A

M

M

Y

Y

K

K

L

I

T

V

D

N

M

G

V

V

M

M

K

Q

L

Y

A

A

P

P

Y

I

G

G

V

V

F

F

G

A

L

L

M

I

A

A

W

Y

V

V

A

M

G

A

E

E

Y

Q

G

G

M

V

D

K

M

V

L

V

M

G

P

E

L

L

I

A

K

K

V

V

I

T

L

A

A

A

V

V

Y

Y

I

V

G

G

C

L

A

T

L

L

H

Q

I

I

I

L

G

L

F

V

Y

Y

S

-

L

F

V

V

L

L

T

L

F

K

V

I

A

Y

K

G

L

I

N

D

P

P

M

I

Q

S

F

F

F

I

K

K

G

K

I

A

S

K

N

D

A

A

L

M

A

L

V

T

A

A

Y

F

T

V

T

T

S
x
R

S

S

S
|
S

A

S

G

G

T

T

L

L

P

P

A

V

S

T

M

M

K

R

C

V

A

A

S

K

E

E

S

-

L

M

G

G

I

I

N

S

K

E

K

G

I

I

S

Y

S

S

F

F

V

T

L

L

P

P

L

L

G

G

T

A

T

T

I

I

N

N

M

M

D

D

G

G

T
|
T

A

A

L

L

Y
|
Y

Q

Q

G

G

V

V

T

C

A

T

L

F

F

F

V

I

A

A

Q

N

A

A

F

L

G

G

I

S

D

H

L

L

T

T

W

V

V

G

D

Q

Y

Q

I

L

T

T

I

I

I

V

L

L

T

T

A

A

T

V

L

L

A

A

S

S

I

I

G

G

T

T

A

A

G

G

V

V

P

P

G

G

A

A

G

G

L

A

V

I

M

M

L

L

T

A

L

M

V

V

L

L

T

E

T

S

V

V

G

G

L

L

P

P

L

L

E

T

-

D

-

P

-

N

-

V

-

A

G

A

V

Y

A

A

I

M

I

I

A

L

G

G

I

I

D
|
D

R

A

I

I

L

L

D
|
D

M

M

A

G

R
x
C

T
|
T

V

M

V

V

N

N

V

V

S

T

G

G

D

D

L

L

V

T

A

G

T

T

T

A

V

I

I

V

A

A

K

K

S

T

E

E

binding benzylcysteine: R268 (≠ S274), S270 (= S276), T306 (= T313), Y309 (= Y316), D382 (= D383), D386 (= D387), C389 (≠ R390), T390 (= T391)

6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
46% identity, 90% coverage: 21:409/433 of query aligns to 3:408/408 of 6bauA

query
sites
6bauA

P

P

L

V

W

L

L

Q

Q

K

I

I

L

L

I

I

G

G

M

L

L

I

L

L

G

G

I

A

C

I

A

V

G

G

L

L

G

I

L

L

G

G

E

H

Q

Y

A

G

-

Y

-

A

-

D

-

A

-

V

-

K

V

T

L

Y

L

V

K

K

P

P

I

F

G

G

T

D

L

L

F

F

V

V

N

R

T

L

I

L

K

K

M

M

L

L

I

V

V

M

P

P

L

I

V

V

F

F

C

A

S

S

L

L

I

V

V

V

G

G

V

A

T

A

S

S

M

I

Q

S

D

P

T

-

A

A

K

R

M

L

G

G

R

R

I

V

G

G

F

V

K

K

S

I

F

V

A

V

F

Y

Y

Y

L

L

G

L

T

T

T

S

S

A

I

F

A

A

I

V

T

T

L

L

G

G

L

I

A

I

V

M

G

A

H

R

I

L

M

F

Q

N

P

P

G

G

A

A

G

G

L

I

A

H

M

L

T

A

S

V

A

G

E

G

S

Q

H

Q

N

F

A

Q

V

P

K

K

E

Q

V

A

P

P

S

P

I

L

M

V

E

K

T

I

L

L

I

L

N

D

I

I

V

V

P

P

T

T

N

N

P

P

I

F

A

G

A

A

L

L

A

A

S

N

G

G

Q

Q

I

V

L

L

Q

P

V

T

I

I

V

F

F

F

A

A

V

I

A

I

L

L

G

G

I

I

A

A

L

I

V

T

L

Y

I

L

G

M

D

N

H

S

G

E

K

N

P

E

A

K

I

V

K

R

-

K

-

S

-

A

-

E

-

T

-

L

-

D

V

A

F

I

E

N

S

G

L

L

A

A

E

E

A

A

M

M

Y

Y

K

K

L

I

T

V

D

N

M

G

V

V

M

M

K

Q

L

Y

A

A

P

P

Y

I

G

G

V

V

F

F

G

A

L

L

M

I

A

A

W

Y

V

V

A

M

G

A

E

E

Y

Q

G

G

M

V

D

K

M

V

L

V

M

G

P

E

L

L

I

A

K

K

V

V

I

T

L

A

A

A

V

V

Y

Y

I

V

G

G

C

L

A

T

L

L

H

Q

I

I

I

L

G

L

F

V

Y

Y

S

-

L

F

V

V

L

L

T

L

F

K

V

I

A

Y

K

G

L

I

N

D

P

P

M

I

Q

S

F

F

F

I

K

K

G

K

I

A

S

K

N

D

A

A

L

M

A

L

V

T

A

A

Y

F

T

V

T

T

S

R

S

S

S
|
S

A

S

G

G

T

T

L

L

P

P

A

V

S

T

M

M

K

R

C

V

A

A

S

K

E

E

S

-

L

M

G

G

I

I

N

S

K

E

K

G

I

I

S

Y

S

S

F

F

V

T

L

L

P

P

L

L

G

G

T

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T
|
T

A

A

L

L

Y

Y

Q

Q

G

G

V

V

T

C

A

T

L

F

F

F

V

I

A

A

Q

N

A

A

F

L

G

G

I

S

D

H

L

L

T

T

W

V

V

G

D

Q

Y

Q

I

L

T

T

I

I

I

V

L

L

T

T

A

A

T

V

L

L

A

A

S

S

I

I

G

G

T

T

A
|
A

G
|
G

V
|
V

P

P

G

G

A

A

G
|
G

L

A

V

I

M

M

L

L

T

A

L

M

V

V

L

L

T

E

T

S

V

V

G

G

L

L

P

P

L

L

E

T

-

D

-

P

-

N

-

V

-

A

G

A

V

Y

A

A

I

M

I

I

A

L

G

G

I

I

D

D

R

A

I

I

L

L

D
|
D

M

M

A

G

R
x
C

T
|
T

V

M

V

V

N
|
N

V

V

S

T

G

G

D

D

L

L

V

T

A

G

T

T

T

A

V

I

I

V

A

A

K

K

S

T

E

E

binding cysteine: S270 (= S276), M303 (= M310), T306 (= T313), A345 (= A352), G346 (= G353), V347 (= V354), G351 (= G358), D386 (= D387), C389 (≠ R390), T390 (= T391), N393 (= N394)

6bmiA Crystal structure of gltph r397c in complex with l-serine (see paper)
43% identity, 90% coverage: 21:409/433 of query aligns to 3:396/396 of 6bmiA

query
sites
6bmiA

P

P

L

V

W

L

L

Q

Q

K

I

I

L

L

I

I

G

G

M

L

L

I

L

L

G

G

I

A

C

I

A

V

G

G

L

L

G

I

L

L

G

G

E

H

Q

Y

A

G

-

Y

-

A

-

D

-

A

-

V

-

K

V

T

L

Y

L

V

K

K

P

P

I

F

G

G

T

D

L

L

F

F

V

V

N

R

T

L

I

L

K

K

M

M

L

L

I

V

V

M

P

P

L

I

V

V

F

F

C

A

S

S

L

L

I

V

V

V

G

G

V

A

T

A

S

S

M

I

Q

S

D

P

T

-

A

A

K

R

M

L

G

G

R

R

I

V

G

G

F

V

K

K

S

I

F

V

A

V

F

Y

Y

Y

L

L

G

L

T

T

T

S

S

A

I

F

A

A

I

V

T

T

L

L

G

G

L

I

A

I

V

M

G

A

H

R

I

L

M

F

Q

N

P

P

G

G

A

A

G

G

L

I

A

H

M

L

T

A

S

V

A

G

E

G

S

Q

H

Q

N

F

A

Q

V

P

K

K

E

Q

V

A

P

P

S

P

I

L

M

V

E

K

T

I

L

L

I

L

N

D

I

I

V

V

P

P

T

T

N

N

P

P

I

F

A

G

A

A

L

L

A

A

S

N

G

G

Q

Q

I

V

L

L

Q

P

V

T

I

I

V

F

F

F

A

A

V

I

A

I

L

L

G

G

I

I

A

A

L

I

V

T

L

Y

I

L

G

M

D

N

H

S

G

E

K

N

P

E

A

K

I

V

K

R

-

K

-

S

-

A

-

E

-

T

-

L

-

D

V

A

F

I

E

N

S

G

L

L

A

A

E

E

A

A

M

M

Y

Y

K

K

L

I

T

V

D

N

M

G

V

V

M

M

K

Q

L

Y

A

A

P

P

Y

I

G

G

V

V

F

F

G

A

L

L

M

I

A

A

W

Y

V

V

A

M

G

A

E

E

Y

Q

G

G

M

V

D

K

M

V

L

V

M

G

P

E

L

L

I

A

K

K

V

V

I

T

L

A

A

A

V

V

Y

Y

I

V

G

G

C

L

A

T

L

L

H

Q

I

I

I

L

G

L

F

V

Y

Y

S

-

L

F

V

V

L

L

T

L

F

K

V

I

A

Y

K

G

L

I

N

D

P

P

M

I

Q

S

F

F

F

I

K

K

G

K

I

A

S

K

N

D

A

A

L

M

A

L

V

T

A

A

Y

F

T

V

T

T

S
x
R

S

S

S
|
S

A

S

G

G

T

T

L

L

P

P

A

V

S

T

M

M

K

R

C

V

A

A

S

K

E

E

S

-

L

M

G

G

I

I

N

S

K

E

K

G

I

I

S

Y

S

S

F

F

V

T

L

L

P

P

L

L

G

G

T

A

T

T

I

I

N

N

M

M

D

D

G

G

T
|
T

A

A

L

L

Y

Y

Q

Q

G

G

V

V

T

C

A

T

L

F

F

F

V

I

A

A

Q

N

A

A

F

L

G

G

I

S

D

H

L

L

T

T

W

V

V

G

D

Q

Y

Q

I

L

T

T

I

I

I

V

L

L

T

T

A

A

T

V

L

L

A

A

S

-

I

-

G

-

T

-

A

-

G

-

V

-

P

-

G

-

A

-

G

-

L

-

V

I

M

M

L

L

T

A

L

M

V

V

L

L

T

E

T

S

V

V

G

G

L

L

P

P

L

L

E

T

-

D

-

P

-

N

-

V

-

A

G

A

V

Y

A

A

I

M

I

I

A

L

G

G

I

I

D

D

R

A

I

I

L

L

D
|
D

M

M

A

G

R

C

T
|
T

V

M

V

V

N
|
N

V

V

S

T

G

G

D

D

L

L

V

T

A

G

T

T

T

A

V

I

I

V

A

A

K

K

S

T

E

E

binding serine: R268 (≠ S274), S270 (= S276), T306 (= T313), D374 (= D387), T378 (= T391), N381 (= N394)

7awmA Structure of the thermostabilized eaat1 cryst mutant in complex with l-asp, three sodium ions and the allosteric inhibitor ucph101 (see paper)
38% identity, 86% coverage: 51:422/433 of query aligns to 57:412/412 of 7awmA

query
sites
7awmA

G

G

T

E

L

L

F

L

V

M

N

R

T

M

I

L

K

K

M

M

L

L

I

I

V

L

P

P

L

L

V

I

F

V

C

S

S

S

L

L

I

I

V

T

G

G

V

L

T

A

S

S

M

L

Q

-

D

D

T

A

A

K

K

A

M
x
S

G
|
G

R

R

I

L

G
|
G

F

M

K

R

S
x
A

F
x
V

A

V

F

Y

Y
|
Y

L

M

G

S

T

T

S

I

I

I

A

A

I

V

T

V

L

L

G

G

L

I

A

I

V

L

G

V

H

L

I

I

M

I

Q

H

P

P

G

-

A

-

G

-

L

-

A

-

M

-

T

-

S

-

A

-

E

-

S

-

H

-

N

-

A

-

V

-

K

E

E

V

V

L

P

D

S

C

I

F

M

L

E

D

T

L

L

A

I

R

N

N

I

I

V

F

P

P

T

S

N

N

P

L

I

V

A

S

A

A

-

A

-

F

-

R

-

S

-

Y

-

S

-

T

-

Q

-

E

L

V

A

E

S

G

G

M

Q

N

I

I

L

L

Q

G

V

L

I

V

V

V

F

F

A

S

V
x
M

A

V

L

F

G

G

I
x
F

A

A

L

L

V

G

L

K

I

M

G

G

D

E

H

Q

G

G

K

Q

P

L

A

L

I

V

K

D

V

F

F

F

E

N

S

S

L

L

A

N

E

E

A

A

M

T

Y

M

K

K

L

L

T

V

D

A

M

I

V

I

M

M

K

W

L

Y

A

A

P

P

Y

L

G

G

V

I

F

L

G

F

L

L

M

I

A

A

-

G

-

K

W

I

V

V

A

G

G

G

E

Q

Y

L

G

G

M

M

D

Y

M

M

L

V

M

-

P

-

L

-

I

-

K

-

V

-

I

-

L

-

A

T

V

V

Y

I

I

V

G

G

C

L

A

V

L

I

H

H

I

G

I

L

G

I

F

V

Y

L

S

P

L

L

V

I

L

Y

T

F

F

L

V

I

A

T

K

R

L

K

N

N

P

P

M

F

Q

V

F

F

F

I

K

A

G

G

I

I

S

L

N

Q

A

A

L

L

A

I

V

T

A

A

Y

L

T

G

T

T

S

S

S
|
S

A

S

G

A

T

T

L

L

P

P

A

I

S

T

M

F

K

K

C

C

A

L

S

E

E

E

S

N

L

N

G

G

I

V

N

D

K

K

K

R

I

I

S

T

S

R

F
|
F

V

V

L

L

P

P

L
x
V

G

G

T

A

T

T

I

I

N

N

M

M

D

D

G

G

T
|
T

A

A

L

L

Y

Y

Q

E

G

A

V

V

T

A

A

A

L

I

F

F

V

I

A

A

Q

Q

A

V

F

N

G

N

I

Y

D

E

L

L

T

D

W

F

V

G

D

Q

Y

I

I

I

T

T

I

I

I

S

L

I

T

T

A

A

T

T

L

A

A

A

S

S

I

I

G

G

T

A

A

A

G
|
G

V
x
I

P

P

G

Q

A
|
A

G
|
G

L

L

V

V

M

T

L

M

T

V

L

I

V

V

L

L

T

T

T

A

V

V

G

G

L

L

P

P

L

T

E

D

G

D

V

I

A

T

I

L

I

I

A

I

G

A

I

V

D

D

R

W

I

L

L

L

D
|
D

M

R

A

F

R
|
R

T
|
T

V

M

V

V

N

N

V

V

S

L

G

G

D

D

L

A

V

L

A

G

T

A

T

G

V

I

I

V

A

E

K

H

-

L

S

S

E

R

D

K

E

E

L

L

D

E

L

-

E

-

H

-

Y

-

N

K

A

Q

D

D

A

A

E

E

binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: S88 (≠ M83), G89 (= G84), G92 (= G87), A95 (≠ S90), V96 (≠ F91), Y99 (= Y94), M163 (≠ V164), F167 (≠ I168), F293 (= F300), V297 (≠ L304)
binding aspartic acid: S268 (= S275), S269 (= S276), T306 (= T313), G346 (= G353), I347 (≠ V354), A350 (= A357), G351 (= G358), D380 (= D387), R383 (= R390), T384 (= T391)

5mjuA Structure of the thermostabilized eaat1 cryst mutant in complex with the competititve inhibitor tfb-tboa and the allosteric inhibitor ucph101 (see paper)
38% identity, 84% coverage: 51:413/433 of query aligns to 49:393/397 of 5mjuA

query
sites
5mjuA

G

G

T

E

L

L

F

L

V

M

N

R

T

M

I

L

K

K

M

M

L

L

I

I

V

L

P

P

L

L

V
x
I

F

V

C

S

S

S

L

L

I

I

V

T

G

G

V
x
L

T

A

S

S

M

L

Q

-

D

D

T

A

A

K

K

A

M
x
S

G
|
G

R

R

I

L

G
|
G

F

M

K

R

S

A

F

V

A

V

F

Y

Y
|
Y

L

M

G

S

T

T

S

I

I

I

A

A

I

V

T

V

L

L

G

G

L

I

A

I

V

L

G

V

H

L

I

I

M

I

Q

H

P

-

G

-

A

-

G

-

L

-

A

-

M

-

T

-

S

-

A

-

E

-

S

-

H

-

N

-

A

-

V

-

K

E

E

V

V

L

P

D

S

C

I

F

M

L

E

D

T

L

L

A

I

R

N

N

I

I

V

F

P

P

T

S

N

N

P

L

I

V

A

S

A

A

L

A

-

F

-

R

-

S

-

Y

-

S

A

T

S

T

G

G

Q

Q

-

E

-

V

-

E

-

G

-

M

-

N

I
|
I

L

L

Q

G

V

L

I

V

V

V

F

F

A

S

V
x
M

A

V

L

F

G

G

I
x
F

A

A

L

L

V

G

L

K

I

M

G

G

D

E

H

Q

G

G

K

Q

P

L

A

L

I

V

K

D

V

F

F

F

E

N

S

S

L

L

A

N

E

E

A

A

M

T

Y

M

K

K

L

L

T

V

D

A

M

I

V

I

M

M

K

W

L

Y

A

A

P

P

Y

L

G

G

V

I

F

L

G

F

L

L

M

I

A

A

-

G

-

K

W

I

V

V

A

E

G

G

E

Q

Y

L

G

G

M

M

D

Y

M

M

L

V

M

-

P

-

L

-

I

-

K

-

V

-

I

-

L

-

A

T

V

V

Y

I

I

V

G

G

C

L

A

V

L

I

H

H

I

G

I

L

G

I

F

V

Y

L

S

P

L

L

V

I

L

Y

T

F

F

L

V

I

A

T

K

R

L

K

N

N

P

P

M

F

Q

V

F

F

F

I

K

A

G

G

I

I

S

L

N

Q

A

A

L

L

A

I

V

T

A

A

Y

L

T

G

T

T

S

S

S
|
S

A
x
S

G

A

T

T

L

L

P

P

A

I

S

T

M

F

K

K

C

C

A

L

S

E

E

E

S

N

L

N

G

G

I

V

N

D

K

K

K

R

I

I

S

T

S

R

F
|
F

V

V

L

L

P

P

L
x
V

G

G

T
x
A

T
|
T

I

I

N

N

M
|
M

D

D

G

G

T
|
T

A

A

L

L

Y

Y

Q

E

G

A

V

V

T

A

A

A

L

I

F

F

V

I

A

A

Q

Q

A

V

F

N

G

Q

I

I

D

-

L

-

T

-

W

-

V

-

D

-

Y

-

I

I

T

T

I

I

I

S

L

I

T

T

A

A

T

T

L

A

A

A

S

S

I

I

G
|
G

T

A

A

A

G

G

V

I

P

P

G

Q

A
|
A

G
|
G

L

L

V

V

M

T

L

M

T

V

L

I

V

V

L

L

T

T

T

A

V

V

G

G

L

L

P

P

L

T

E

D

G

D

V

I

A

T

I

L

I

I

A

I

G

A

I

V

D

D

R

W

I

L

L

L

D
|
D

M

R

A

F

R
|
R

T

T

V

M

V

V

N
|
N

V

V

S

L

G

G

D

D

L

A

V

L

A

G

T

A

T

G

V

I

I

V

A

E

K

H

-

L

S

S

E

R

D

K

E

E

L

L

D

E

binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: L72 (≠ V74), S80 (≠ M83), G81 (= G84), G84 (= G87), Y91 (= Y94), M156 (≠ V164), F160 (≠ I168), F286 (= F300), V290 (≠ L304)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I64 (≠ V66), I148 (= I156), S262 (= S276), S263 (≠ A277), A292 (≠ T306), T293 (= T307), M296 (= M310), T299 (= T313), G329 (= G350), A336 (= A357), G337 (= G358), D366 (= D387), R369 (= R390), N373 (= N394)

7xr6A Structure of human excitatory amino acid transporter 2 (eaat2) in complex with way-213613 (see paper)
36% identity, 84% coverage: 51:413/433 of query aligns to 47:419/424 of 7xr6A

query
sites
7xr6A

G

G

T

D

L

I

F

L

V

M

N

R

T

M

I

L

K

K

M

M

L

L

I

I

V

L

P

P

L

L

V

I

F

I

C

S

S

S

L

L

I

I

V

T

G

G

V

L

T

S

S

G

M

L

Q

-

D

D

T

A

A

K

K

A

M

S

G

G

R
|
R

I

L

G

G

F

T

K
x
R

S

A

F

M

A

V

F

Y

Y

Y

L

M

G

S

T

T

S

I

I
|
I

A

A

I

A

T

V

L

L

G

G

L

V

A

I

V

L

G

V

H

L

I

A

M

I

Q

H

P

P

G

G

A

-

G

-

L

-

A

-

M

-

T

-

S

-

A

-

E

-

S

-

H

N

N

P

A

K

V

V

K

S

E

S

V

L

P

D

S

A

I

F

M

L

E

D

T

L

L

I

I

R

N

N

I

L

V

F

P

P

T

E

N

N

P

L

I

V

A

Q

A

A

-

C

-

F

-

Q

-

I

-

Q

-

T

-

V

-

T

-

K

-

V

L

I

A

K

S

K

G

G

-

L

-

E

-

F

-

K

-

D

-

G

-

M

Q

N

I

V

L

L

Q

G

V

L

I

I

V

G

F

F

A

F

V

I

A

A

L

F

G

G

I

I

A

A

L

M

V

G

L

K

I

M

G

G

D

D

H

Q

G

A

K

K

P

L

A

M

I

V

K

D

V

F

F

F

E

N

S

I

L

L

A

N

E

E

A

I

M

V

Y

M

K

K

L

L

T

V

D

I

M

M

V

I

M

M

K

W

L

Y

A

S

P

P

Y

L

G

G

V

I

F

A

G

C

L

L

M

I

A

C

W

G

-

K

-

I

-

A

-

I

-

K

-

D

-

L

-

E

-

V

V

V

A

A

G

R

E

Q

Y

L

G

G

M

M

D

Y

M

M

L

V

M

-

P

-

L

-

I

-

K

-

V

-

I

-

L

-

A

T

V

V

Y

I

I

I

G

G

C

L

A

I

L

I

H

H

I

G

G

I

F

F

Y

L

S

P

L

L

V
x
I

L

Y

T

F

F

V

V

V

A

T

K

R

L

K

N

N

P

P

M

F

Q

S

F

F

K

A

G

G

I

I

S

F

N

Q

A

A

L

W

A

I

V

T

A

A

Y

L

T

G

T

T

S

A

S
|
S

A

A

G

G

T

T

L

L

P

P

A

V

S

T

M

F

K

R

C

C

A

L

S

E

E

E

S

N

L

L

G

G

I

I

N

D

K

K

K

R

I

V

S

T

S

R

F

F

V

V

L

L

P

P

L

V

G

G

T

A

T

T

I

I

N

N

M

M

D

D

G

G

T
|
T

A

A

L

L

Y

Y

Q

E

G

A

V

V

T

A

A

A

L

I

F

F

V

I

A

A

Q

Q

A

M

F

N

G

G

I

V

D

V

L

L

T

D

W

G

V

G

D

Q

Y

I

I

V

T

T

I

V

I

S

L

L

T

T

A

A

T

T

L

L

A

A

S

S

I

V

G

G

T

A

A

A

G

S

V

I

P

P

G

S

A

A

G
|
G

L

L

V

V

M

T

L
x
M

T

L

L

L

V

I

L

L

T

T

T

A

V

V

G

G

L

L

P

P

L

T

E

E

G

D

V

I

A

S

I

L

A
x
V

G

A

I

V

D
|
D

R
x
W

I

L

L

L

D

D

M

R

A

M

R
|
R

T
|
T

V

S

V

V

N

N

V

V

S

V

G

G

D

D

L

S

V

F

A

G

T

A

T

G

V

I

I

V

A

Y

K

H

-

L

S

S

E

K

D

S

E

E

L

L

D

D

binding (2S)-2-azanyl-4-[[4-[2-bromanyl-4,5-bis(fluoranyl)phenoxy]phenyl]amino]-4-oxidanylidene-butanoic acid: S280 (= S275), S281 (= S276), T318 (= T313), G363 (= G358), M367 (≠ L362), V385 (≠ A380), D388 (= D383), R395 (= R390), T396 (= T391)
binding dodecyl beta-D-glucopyranoside: W389 (≠ R384)
binding cholesterol hemisuccinate: R80 (= R85), R84 (≠ K89), I95 (= I100), I252 (≠ V247)

Sites not aligning to the query:

binding dodecyl beta-D-glucopyranoside: 16, 19, 20

7xr4A Structure of human excitatory amino acid transporter 2 (eaat2) in complex with glutamate (see paper)
36% identity, 84% coverage: 51:413/433 of query aligns to 46:420/425 of 7xr4A

query
sites
7xr4A

G

G

T

D

L

I

F

L

V

M

N

R

T

M

I

L

K

K

M

M

L

L

I

I

V

L

P

P

L

L

V

I

F

I

C

S

S

S

L

L

I

I

V

T

G

G

V

L

T

S

S

G

M

L

Q

-

D

D

T

A

A

K

K

A

M

S

G

G

R
|
R

I

L

G

G

F

T

K

R

S

A

F

M

A

V

F

Y

Y

Y

L

M

G

S

T

T

S

I

I

I

A

A

I

A

T

V

L

L

G

G

L

V

A

I

V

L

G

V

H

L

I

A

M

I

Q

H

P

P

G

G

A

-

G

-

L

-

A

-

M

-

T

-

S

-

A

-

E

-

S

-

H

N

N

P

A

K

V

V

K

S

E

S

V

L

P

D

S

A

I

F

M

L

E

D

T

L

L

I

I

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N

N

I

L

V

F

P

P

T

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N

N

P

L

I

V

A

Q

A

A

-

C

-

F

-

Q

-

I

-

Q

-

T

-

V

-

T

-

K

-

V

L

I

A

K

S

K

G

G

-

L

-

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-

F

-

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-

D

-

G

-

M

Q

N

I

V

L

L

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G

V

L

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I

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F

F

A

F

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A

A

L

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G

G

I

I

A

A

L

M

V

G

L

K

I

M

G

G

D

D

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Q

G

A

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K

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L

A

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I

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K

D

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F

F

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N

S

I

L

L

A

N

E

E

A

I

M

V

Y

M

K

K

L

L

T

V

D

I

M

M

V

I

M

M

K

W

L

Y

A

S

P

P

Y

L

G

G

V

I

F

A

G

C

L

L

M

I

A

C

W

G

-

K

-

I

-

A

-

I

-

K

-

D

-

L

-

E

-

V

V

V

A

A

G

R

E

Q

Y

L

G

G

M

M

D

Y

M

M

L

V

M

-

P

-

L

-

I

-

K

-

V

-

I

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L

-

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T

V

V

Y

I

I

I

G

G

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L

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H

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G

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F

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L

L

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x
I

L

Y

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F

F

V

V

V

A

T

K

R

L

K

N

N

P

P

M

F

Q

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F

F

K

A

G

G

I

I

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N

Q

A

A

L

W

A

I

V

T

A

A

Y

L

T

G

T

T

S

A

S

S

S
|
S

A

A

G

G

T

T

L

L

P

P

A

V

S

T

M

F

K

R

C

C

A

L

S

E

E

E

S

N

L

L

G

G

I

I

N

D

K

K

K

R

I

V

S

T

S

R

F

F

V

V

L

L

P

P

L

V

G

G

T

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T
|
T

A

A

L

L

Y

Y

Q

E

G

A

V

V

T

A

A

A

L

I

F

F

V

I

A

A

Q

Q

A

M

F

N

G

G

I

V

D

V

L

L

T

D

W

G

V

G

D

Q

Y

I

I

V

T

T

I

V

I

S

L

L

T

T

A

A

T

T

L

L

A

A

S

S

I

V

G

G

T

A

A

A

G
x
S

V

I

P

P

G

S

A

A

G
|
G

L

L

V

V

M

T

L

M

T

L

L

L

V

I

L

L

T

T

T

A

V

V

G

G

L

L

P

P

L

T

E

E

G

D

V

I

A

S

I

L

A

V

G

A

I

V

D

D

R

W

I

L

L

L

D
|
D

M

R

A

M

R
|
R

T
|
T

V

S

V

V

N
|
N

V

V

S

V

G

G

D

D

L

S

V

F

A

G

T

A

T

G

V

I

I

V

A

Y

K

H

-

L

S

S

E

K

D

S

E

E

L

L

D

D

binding glutamic acid: S282 (= S276), M316 (= M310), T319 (= T313), S359 (≠ G353), G364 (= G358), D393 (= D387), R396 (= R390), T397 (= T391), N400 (= N394)
binding cholesterol hemisuccinate: R79 (= R85), I253 (≠ V247)

P31596 Excitatory amino acid transporter 2; GLT-1; Sodium-dependent glutamate/aspartate transporter 2; GLUT-R; Solute carrier family 1 member 2 from Rattus norvegicus (Rat) (see paper)
33% identity, 87% coverage: 51:425/433 of query aligns to 82:517/573 of P31596

query
sites
P31596

G

G

T

D

L

I

F

L

V

M

N

R

T

M

I

L

K

K

M

M

L

L

I

I

V

L

P

P

L

L

V

I

F

I

C

S

S

S

L

L

I

I

V

T

G

G

V

L

T

S

S

G

M

L

Q

-

D

D

T

A

A

K

K

A

M

S

G

G

R

R

I

L

G

G

F

T

K

R

S

A

F

M

A

V

F

Y

Y

Y

L

M

G

S

T

T

S

I

I

I

A

A

I

A

T

V

L

L

G

G

L

V

A

I

V

L

G

V

H

L

I

A

M

I

Q

H

P

P

G

G

A

N

G

P

-

K

-

L

-

K

-

Q

-

L

-

G

-

P

-

G

-

K

-

N

-

D

-

E

-

V

-

S

-

L

-

D

-

A

-

F

-

L

-

D

-

L

-

I

-

R

-

N

-

L

-

F

-

P

-

E

-

N

-

L

-

V

-

Q

-

A

-

C

-

F

-

Q

-

I

-

Q

-

T

-

V

-

T

-

K

-

V

L

L

A

V

M

A

T

P

S

P

A

S

E

E

S

E

H

A

N

N

A

T

V

T

K

K

E

A

V

V

P

I

S

S

I

L

M

L

E

N

T

E

L

T

I

M

N

N

I

E

V

A

P

P

T

E

N

E

P

T

I

K

A

I

A

V

L

I

A

K

S

K

G

G

-

L

-

E

-

F

-

K

-

D

-

G

-

M

Q

N

I

V

L

L

Q

G

V

L

I

I

V

G

F

F

A

F

V

I

A

A

L

F

G

G

I

I

A

A

L

M

V

G

L

K

I

M

G

G

D

E

H

Q

G

A

K

K

P

L

A

M

I

V

K

E

V

F

F

F

E

N

S

I

L

L

A

N

E

E

A

I

M

V

Y

M

K

K

L

L

T

V

D

I

M

M

V

I

M

M

K

W

L

Y

A

S

P

P

Y

L

G

G

V

I

F

A

G

C

L

L

M

I

A

C

W

G

-
x
K

-

I

-

A

-

I

-

K

-

D

-

L

-

E

-

V

V

V

A

A

G

R

E

Q

Y

L

G

G

M

M

D

Y

M

M

L

I

M

-

P

-

L

-

I

-

K

-

V

-

I

-

L

-

A

T

V

V

Y

I

I

V

G

G

C

L

A

I

L

I

H
|
H

I

G

G

I

F

F

Y

L

S

P

L

L

V

I

L

Y

T

F

F

V

V

V

A

T

K

R

L

K

N

N

P

P

M

F

Q

S

F

F

K

A

G

G

I

I

S

F

N

Q

A

A

L

W

A

I

V

T

A

A

Y

L

T

G

T

T

S

A

S

S

A

A

G

G

T

T

L

L

P

P

A

V

S

T

M

F

K

R

C

C

A

L

S

E

E

D

S

N

L

L

G

G

I

I

N

D

K

K

K

R

I

V

S

T

S

R

F

F

V

V

L

L

P

P

L

V

G

G

T

A

T

T

I

I

N

N

M

M

D

D

G

G

T

T

A

A

L

L

Y

Y

Q

E

G

A

V

V

T

A

A

A

L

I

F

F

V

I

A

A

Q

Q

A

M

F

N

G

G

I

V

D

I

L

L

T

D

W

G

V

G

D

Q

Y

I

I

V

T

T

I

V

I

S

L

L

T

T

A

A

T

T

L

L

A

A

S

S

I

I

G

G

T

A

A

A

G

S

V

I

P

P

G

S

A

A

G

G

L

L

V

V

M

T

L

M

T

L

L

L

V

I

L

L

T

T

T

A

V

V

G

G

L

L

P

P

L

T

E

E

G

D

V

I

A

S

I

L

A

V

G

A

I

V

D

D

R

W

I

L

L

L

D

D

M

R

A

M

R

R

T

T

V

S

V

V

N

N

V

V

S

V

G

G

D

D

L

S

V

F

A

G

T

A

T

G

V

I

I

V

-

Y

-

H

-

L

A

S

K

K

S

S

E

E

-

L

D

D

E

T

L

I

D

D

L

S

E

Q

H

H

Y

R

-

M

N

H

A

E

D

D

A

I

E

E

Q

M

S

T

Q

K

K298 (vs. gap) mutation K->H,R: Normal transporter activity.; mutation K->N,T: Reduced transporter activity.
H326 (= H239) mutation H->N,T,K,R: No transporter activity.

P43006 Excitatory amino acid transporter 2; GLT-1; Sodium-dependent glutamate/aspartate transporter 2; Solute carrier family 1 member 2 from Mus musculus (Mouse) (see paper)
33% identity, 87% coverage: 51:425/433 of query aligns to 82:517/572 of P43006

query
sites
P43006

G

G

T

D

L

I

F

L

V

M

N

R

T

M

I

L

K

K

M

M

L

L

I

I

V

L

P

P

L

L

V

I

F

I

C

S

S

S

L

L

I

I

V

T

G

G

V

L

T

S

S

G

M

L

Q

-

D

D

T

A

A

K

K

A

M

S

G

G

R

R

I

L

G

G

F

T

K

R

S

A

F

M

A

V

F

Y

Y

Y

L

M

G

S

T

T

S

I

I

I

A

A

I

A

T

V

L

L

G

G

L

V

A

I

V

L

G

V

H

L

I

A

M

I

Q

H

P

P

G

G

A

N

G

P

-

K

-

L

-

K

-

Q

-

L

-

G

-

P

-

G

-

K

-

N

-

D

-

E

-

V

-

S

-

L

-

D

-

A

-

F

-

L

-

D

-

L

-

I

-

R

-

N

-

L

-

F

-

P

-

E

-

N

-

L

-

V

-

Q

-

A

-

C

-

F

-

Q

-

I

-

Q

-

T

-

V

-

T

-

K

-

V

L

L

A

V

M

A

T

P

S

P

A

S

E

E

S

E

H

A

N

N

A

T

V

T

K

K

E

A

V

V

P

I

S

S

I

M

M

L

E

N

T

E

L

T

I

M

N

N

I

E

V

A

P

P

T

E

N

E

P

T

I

K

A

I

A

V

L

I

A

K

S

K

G

G

-

L

-

E

-

F

-

K

-

D

-

G

-

M

Q

N

I

V

L

L

Q

G

V

L

I

I

V

G

F

F

A

F

V

I

A

A

L

F

G

G

I

I

A

A

L

M

V

G

L

K

I

M

G

G

D

E

H

Q

G

A

K

K

P

L

A

M

I

V

K

E

V

F

F

F

E

N

S

I

L

L

A

N

E

E

A

I

M

V

Y

M

K

K

L

L

T

V

D

I

M

M

V

I

M

M

K

W

L

Y

A

S

P

P

Y

L

G

G

V

I

F

A

G

C

L

L

-

I

-

C

-

G

-

K

-

I

-

A

-

I

-

K

-

D

M

L

A

E

W

V

V

V

A

A

G

R

E

Q

Y

L

G

G

M

M

D

Y

M

M

L

I

M

-

P

-

L

-

I

-

K

-

V

-

I

-

L

-

A

T

V

V

Y

I

I

V

G

G

C

L

A

I

L

I

H

H

I

G

G

I

F

F

Y

L

S

P

L

L

V

I

L

Y

T

F

F

V

V

V

A

T

K

R

L

K

N

N

P

P

M

F

Q

S

F

F

K

A

G

G

I

I

S

F

N

Q

A

A

L

W

A

I

V

T

A

A

Y

L

T

G

T

T

S

A

S

S

A

A

G

G

T

T

L

L

P

P

A

V

S

T

M

F

K

R

C

C

A

L

S

E

E

D

S

N

L

L

G

G

I

I

N

D

K

K

K

R

I

V

S

T

S

R

F

F

V

V

L

L

P

P

L

V

G

G

T

A

T

T

I

I

N

N

M

M

D

D

G

G

T

T

A

A

L

L

Y

Y

Q

E

G

A

V

V

T

A

A

A

L

I

F

F

V

I

A

A

Q

Q

A

M

F

N

G

G

I

V

D

I

L

L

T

D

W

G

V

G

D

Q

Y

I

I

V

T

T

I

V

I

S

L

L

T

T

A

A

T

T

L

L

A

A

S

S

I

I

G

G

T

A

A

A

G

S

V

I

P

P

G

S

A

A

G

G

L

L

V

V

M

T

L

M

T

L

L

L

V

I

L

L

T

T

T

A

V

V

G

G

L

L

P

P

L

T

E

E

G

D

V

I

A

S

I

L

A

V

G

A

I

V

D

D

R

W

I

L

L

L

D

D

M

R

A

M

R

R

T

T

V

S

V

V

N

N

V

V

S

V

G

G

D

D

L

S

V

F

A

G

T

A

T

G

V

I

I

V

-

Y

-

H

-

L

A

S

K

K

S

S

E

E

-

L

D

D

E

T

L

I

D

D

L

S

E

Q

H

H

Y

R

-

M

N

Q

A

E

D

D

A

I

E

E

Q

M

S

T

Q

K

Sites not aligning to the query:

38 modified: S-palmitoyl cysteine; C→S: Severely impairs glutamate uptake activity.

Q10901 Excitatory amino acid transporter; Sodium-dependent glutamate/ aspartate transporter from Caenorhabditis elegans (see paper)
33% identity, 93% coverage: 26:428/433 of query aligns to 29:480/503 of Q10901

query
sites
Q10901

I

V

L

V

I

L

G

G

M

A

L

L

L

C

G

G

-

F

I

L

C

L

A

R

G

G

L

L

G

Q

L

L

G

S

E

P

Q

Q

A

N

V

I

L

M

L

Y

K

I

P

S

I

F

-

P

G

G

T

E

L

L

F

L

V

M

N

H

T

M

I

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-

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A

N177 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine
N187 (≠ S153) modified: carbohydrate, N-linked (GlcNAc...) asparagine

7vr7A Inward-facing structure of human eaat2 in the way213613-bound state (see paper)
37% identity, 83% coverage: 51:409/433 of query aligns to 39:402/402 of 7vr7A

query
sites
7vr7A

G

G

T

D

L

I

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N

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M

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S
|
S

L
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L

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x
L

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M

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x
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E

E

binding (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en: S57 (= S69), L58 (= L70), L65 (≠ M77), V339 (≠ I349), G340 (= G350), S343 (≠ G353), I344 (≠ V354)
binding cholesterol: W188 (≠ K200), I227 (≠ A237), F250 (= F260), W257 (≠ L267), M379 (≠ A389), S382 (≠ V392)
binding (2S)-2-azanyl-4-[[4-[2-bromanyl-4,5-bis(fluoranyl)phenoxy]phenyl]amino]-4-oxidanylidene-butanoic acid: S266 (= S276), M300 (= M310), T303 (= T313), Y306 (= Y316), G348 (= G358), L349 (= L359), M352 (≠ L362), I366 (≠ V376), L369 (≠ I379), V370 (≠ A380), D373 (= D383), D377 (= D387), R380 (= R390), T381 (= T391), N384 (= N394)

Sites not aligning to the query:

binding cholesterol: 2

Query Sequence

>5208231 FitnessBrowser__PV4:5208231
MMRQQTSSWLGRAWSGWMSVPLWLQILIGMLLGICAGLGLGEQAVLLKPIGTLFVNTIKM
LIVPLVFCSLIVGVTSMQDTAKMGRIGFKSFAFYLGTTSIAITLGLAVGHIMQPGAGLAM
TSAESHNAVKEVPSIMETLINIVPTNPIAALASGQILQVIVFAVALGIALVLIGDHGKPA
IKVFESLAEAMYKLTDMVMKLAPYGVFGLMAWVAGEYGMDMLMPLIKVILAVYIGCALHI
IGFYSLVLTFVAKLNPMQFFKGISNALAVAYTTSSSAGTLPASMKCASESLGINKKISSF
VLPLGTTINMDGTALYQGVTALFVAQAFGIDLTWVDYITIILTATLASIGTAGVPGAGLV
MLTLVLTTVGLPLEGVAIIAGIDRILDMARTVVNVSGDLVATTVIAKSEDELDLEHYNAD
AEQSQILAERLDS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory