SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 5208242 FitnessBrowser__PV4:5208242 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0A799 Phosphoglycerate kinase; EC 2.7.2.3 from Escherichia coli (strain K12) (see 3 papers)
73% identity, 100% coverage: 1:391/391 of query aligns to 1:386/387 of P0A799

query
sites
P0A799

M
|
M

A

S

I

V

I

I

N

K

M

M

S

T

A

D

L

L

D

D

L

L

Q

A

G

G

K

K

R

R

V

V

L

F

I

I

R

R

E

A

D

D

L

L

N

N

V

V

P

P

V

V

S

K

N

D

G

G

V

K

V

V

T

T

S

S

D

D

A

A

R

R

L

I

R

R

A

A

S

S

L

L

P

P

T

T

I

I

K

E

L

L

A

A

L

L

E

K

K

Q

G

G

A

A

A

K

V

V

M

M

V

V

M

T

S

S

H

H

L

L

G

G

R

R

P

P

T

T

E

E

G

G

E

E

Y

Y

N

N

S

E

E

E

F

F

S

S

M

L

Q

L

P

P

V

V

V

V

D

N

Y

Y

L

L

E

K

K

D

A
x
K

L

L

D

S

C

N

P

P

V

V

R

R

L

L

A

V

K

K

D

D

Y

Y

L

L

D

D

G

G

V

V

E

D

A

V

N

A

V

E

G

G

E

E

V

L

V

V

V

V

F

L

E

E

N

N

V

V

R

R

F

F

N

N

V

K

G

G

E

E

K

K

K

K

N

D

D

D

E

E

A

T

L

L

A

S

K

K

K

K

L

Y

A

A

A

A

L

L

C

C

D

D

V

V

Y

F

V

V

M

M

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

Q

Q

A

A

S

S

T

T

H

H

G

G

V

I

G

G

L

K

Y

F

A

A

P

D

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V

A

A

C

C

A

A

G

G

P

P

L

L

L

L

A

A

G

A

E

E

L

L

E

D

A

A

L

L

G

G

K

K

A

A

M

L

D

K

N

E

P

P

A

A

R

R

P

P

L

M

V

V

A

A

I

I

V

V

G

G

G

G

S

S

K

K

V

V

S

S

T

T

K

K

L

L

T

T

V

V

L

L

E

D

S

S

L

L

S

S

G

K

I

I

V

A

D

D

Q

Q

L

L

V

I

V

V

G

G

G

G

G

G

I

I

A

A

N

N

T

T

F

F

I

I

A

A

A

A

A

Q

G

G

H

H

N

D

V

V

G

G

K

K

S

S

L

L

Y

Y

E

E

A

A

D

D

L

L

I

V

D

D

E

E

A

A

K

K

R

R

L

L

V

L

A

T

N

T

A

C

Q

-

S

-

R

-

G

-

G

-

D

N

I

I

P

P

V

V

P

P

T

S

D

D

V

V

V

R

V

V

A

A

S

T

E

E

F

F

S

S

P

E

T

T

A

A

S

P

A

A

T

T

L

L

K

K

A

S

V

V

D

N

Q

D

V

V

A

K

D

A

E

D

D

E

M

Q

I

I

F

L

D

D

I

I

G

G

P

D

D

A

S

S

A

A

E

Q

A

E

L

L

A

A

E

E

I

I

L

L

K

K

N

N

A

A

G

K

T

T

I

I

V

L

W

W

N

N

G

G

P

P

V

V

G

G

V

V

F

F

E

E

F

F

D

P

Q

N

F

F

G

R

E

K

G

G

T

T

K

E

R

I

I

V

A

A

Q

N

A

A

I

I

A

A

D

D

S

S

S

E

A

A

F

F

S

S

I

I

A

A

G

G

G

G

G

G

D

D

T

T

L

L

A

A

A

A

V

I

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D

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L

Y

F

D

G

I

I

A

A

D

D

K

K

V

I

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Y

Y

I

I

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S

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T

G

G

G

G

G

G

A

A

F

F

L

L

E

E

F

F

L

V

E

E

G

G

K

K

E

V

L

L

P

P

A

A

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V

A

A

M

M

L

L

E

E

S

E

R

R

G

A

E

K

M1 (= M1) modified: Initiator methionine, Removed
K84 (≠ A84) modified: N6-acetyllysine

1zmrA Crystal structure of the e. Coli phosphoglycerate kinase (see paper)
73% identity, 100% coverage: 2:391/391 of query aligns to 1:385/386 of 1zmrA

query
sites
1zmrA

A

S

I

V

I

I

N

K

M

M

S

T

A

D

L

L

D

D

L

L

Q

A

G

G

K

K

R

R

V

V

L

F

I

I

R

R

E

A

D

D

L

L

N

N

V

V

P

P

V

V

S

K

N

D

G

G

V

K

V

V

T

T

S

S

D

D

A

A

R
|
R

L

I

R

R

A

A

S

S

L

L

P

P

T

T

I

I

K

E

L

L

A

A

L

L

E

K

K

Q

G

G

A

A

A

K

V

V

M

M

V

V

M

T

S

S

H

H

L

L

G

G

R

R

P

P

T

T

E

E

G

G

E

E

Y

Y

N

N

S

E

E

E

F

F

S

S

M

L

Q

L

P

P

V

V

V

V

D

N

Y

Y

L

L

E
x
K

K

D

A

K

L
|
L

D

S

C

N

P

P

V

V

R

R

L

L

A

V

K

K

D

D

Y

Y

L

L

D

D

G

G

V

V

E

D

A

V

N

A

V

E

G

G

E

E

V

L

V

V

V

V

F

L

E

E

N

N

V

V

R

R

F

F

N

N

V

K

G

G

E

E

K

K

K

K

N

D

D

D

E

E

A

T

L

L

A

S

K

K

K

K

L

Y

A

A

A

A

L

L

C

C

D

D

V

V

Y

F

V

V

M

M

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

Q

Q

A

A

S

S

T

T

H

H

G

G

V

I

G

G

L

K

Y

F

A

A

P

D

V

V

A

A

C

C

A

A

G

G

P

P

L

L

L

L

A

A

G

A

E

E

L

L

E

D

A

A

L

L

G

G

K

K

A

A

M

L

D

K

N

E

P

P

A

A

R

R

P

P

L

M

V

V

A

A

I

I

V

V

G

G

G

G

S

S

K
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K

V

V

S

S

T

T

K

K

L

L

T

T

V

V

L

L

E

D

S

S

L

L

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S

G

K

I

I

V

A

D

D

Q

Q

L

L

V

I

V

V

G

G

G

G

G

G

I

I

A

A

N

N

T

T

F

F

I

I

A

A

A

A

A

Q

G

G

H

H

N

D

V

V

G

G

K

K

S

S

L

L

Y

Y

E

E

A

A

D
|
D

L

L

I

V

D
|
D

E

E

A

A

K

K

R

R

L

L

V

L

A

T

N

T

A

C

Q

-

S

-

R

-

G

-

G

-

D

N

I

I

P

P

V

V

P

P

T

S

D

D

V

V

V

R

V

V

A

A

S

T

E

E

F

F

S

S

P

E

T

T

A

A

S

P

A

A

T

T

L

L

K

K

A

S

V

V

D

N

Q

D

V

V

A

K

D

A

E

D

D

E

M

Q

I

I

F

L

D

D

I

I

G

G

P

D

D

A

S

S

A

A

E

Q

A

E

L

L

A

A

E

E

I

I

L

L

K

K

N

N

A

A

G

K

T

T

I

I

V

L

W

W

N

N

G

G

P

P

V

V

G

G

V

V

F

F

E

E

F

F

D

P

Q

N

F

F

G

R

E

K

G

G

T

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K

E

R

I

I

V

A

A

Q

N

A

A

I

I

A

A

D

D

S

S

S

E

A

A

F

F

S

S

I

I

A

A

G

G

G

G

G
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G

D

D

T

T

L

L

A

A

A

A

V

I

D

D

K

L

Y

F

D

G

I

I

A

A

D

D

K

K

V

I

S

S

Y

Y

I

I

S

S

T

T

G

G

G

G

G
|
G

A

A

F

F

L

L

E

E

F

F

L

V

E

E

G

G

K

K

E

V

L

L

P

P

A

A

V

V

A

A

M

M

L

L

E

E

S

E

R

R

G

A

E

K

active site: R35 (= R36), K192 (= K193), G340 (= G346), G363 (= G369)
binding calcium ion: K81 (≠ E82), L84 (= L85), D236 (= D237), D239 (= D240)

4feyA An x-ray structure of a putative phosphogylcerate kinase with bound adp from francisella tularensis subsp. Tularensis schu s4
59% identity, 99% coverage: 1:389/391 of query aligns to 1:389/392 of 4feyA

query
sites
4feyA

M

M

A

S

I

F

I

L

N

T

M

L

S

K

A

D

L

V

D

D

L

L

Q

K

G

D

K

K

R

K

V

V

L

L

I

V

R

R

E

V

D

D

L

F

N

N

V

V

P

P

V

V

S

K

N

D

G

G

V

K

V

V

T

T

S

S

D

K

A

V

R
|
R

L

I

R

E

A

A

S

A

L

I

P

P

T

T

I

I

K

Q

L

Y

A

I

L

L

E

D

K

Q

G

G

A

G

A

A

V

V

M

I

V

L

M

M

S

S

H

H

L

L

G

G

R

R

P

P

T

T

E

E

G

G

E

E

Y

Y

N

D

S

S

E

Q

F

F

S

S

M

L

Q

E

P

P

V

V

V

A

D

K

Y

A

L

L

E

S

K

E

A

I

L

I

D

N

C

K

P

P

V

V

R

K

L

F

A

A

K

K

D

D

Y

W

L

L

D

D

G

G

V

V

E

D

A

V

N

K

V

A

G

G

E

E

V

I

V

V

V

M

F

C

E

E

N

N

V

V

R

R

F

F

N

N

V

S

G

G

E

E

K

K

K

K

N

S

D

T

E

D

A

D

L

L

A

S

K

K

K

K

L

I

A

A

A

S

L

L

C

G

D

D

V

V

Y

F

V

V

M

M

D

D

A

A

F

F

G

A

T

T

A

A

H

H

R

R

A

A

Q

Q

A

A

S

S

T

T

H

Y

G

G

V

V

G

A

L

K

Y

Y

A

I

P

P

V

V

A

A

C

C

A

A

G

G

P

I

L

L

L

L

A

T

G

N

E

E

L

I

E

Q

A

A

L

L

G

E

K

K

A

A

M

L

D

K

N

S

P

P

A

K

R

K

P

P

L

M

V

A

A

A

I

I

V

V

G
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G

G
|
G

S
|
S

K
|
K

V

V

S

S

T

T

K
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K

L

L

T

S

V

V

L

L

E

N

S

N

L

L

S

L

G

D

I

K

V

V

D

E

Q

I

L

L

V

I

V

V

G

G

G
|
G

G
|
G

I

I

A

A

N
|
N

T

T

F

F

I

I

A

K

A

A

A

E

G

G

H

F

N

D

V

V

G

G

K

N

S

S

L
|
L

Y

Y

E

E

A

Q

D

D

L

L

I

V

D

A

E

E

A

A

K

T

R

E

L

I

V

L

A

A

N

K

A

A

Q

K

S

A

R

L

G

G

G

V

D

N

I

I

P

P

V

V

P

P

T

V

D

D

V

V

V

R

V

V

A

A

S

K

E

E

F

F

S

S

P

E

T

N

A

A

S

Q

A

A

T

I

L

I

K

K

A

K

V

V

D

S

Q

D

V

V

A

V

D

A

E

D

D

E

M

M

I
|
I

F
x
L

D

D

I

I

G

G

P

P

D

E

S

S

A

Q

E

K

A

I

L

I

A

A

E

E

I

L

L

L

K

K

N

S

A

A

G

N

T

T

I

I

V

L

W

W

N
|
N

G

G

P
|
P

V

V

G
|
G

V
|
V

F

F

E
|
E

F

F

D

D

Q

N

F

F

G

A

E

E

G

G

T

T

K

K

R

A

I

L

A

S

Q

L

A

A

I

I

A

A

D

Q

S

S

S

H

A

A

F

F

S

S

I

V

A

A

G

G

G
|
G

G
|
G

D
|
D

T

T

L

I

A

A

A

A

V

I

D

E

K

K

Y

F

D

G

I

I

A

K

D

D

K

Q

V

V

S

S

Y

Y

I

I

S

S

T

T

G

A

G

G

G
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G

A

A

F

F

L

L

E

E

F

F

L

L

E

E

G

G

K

K

E

K

L

L

P

P

A

A

V

I

A

E

M

I

L

L

E

K

S

E

R

K

active site: R36 (= R36), K193 (= K193), G346 (= G346), G369 (= G369)
binding adenosine-5'-diphosphate: G190 (= G190), G191 (= G191), S192 (= S192), K197 (= K197), G215 (= G215), G216 (= G216), N219 (= N219), L233 (= L233), I288 (= I288), L289 (≠ F289), N312 (= N312), P314 (= P314), G316 (= G316), V317 (= V317), E319 (= E319), G345 (= G345), G346 (= G346), D347 (= D347)

4ng4B Structure of phosphoglycerate kinase (cbu_1782) from coxiella burnetii (see paper)
55% identity, 99% coverage: 3:391/391 of query aligns to 2:389/389 of 4ng4B

query
sites
4ng4B

I

F

I

L

N

S

M

M

S

S

A

N

L

L

D

N

L

L

Q

H

G

N

K

K

R

R

V

V

L

M

I

I

R

R

E

E

D

D

L

L

N

N

V

V

P

P

V

M

S

K

N

N

G

G

V

K

V

I

T

T

S

N

D

D

A

E

R
|
R

L

I

R

V

A

R

S

A

L

L

P

P

T

T

I

I

K

Q

L

K

A

A

L

I

E

E

K

Q

G

K

A

A

A

R

V

V

M

M

V

I

M

L

S

S

H

H

L

L

G

G

R

R

P

P

T

E

E

E

G

G

E

K

Y

F

N

E

S

K

E

E

F

F

S

S

M

L

Q

A

P

P

V

V

V

A

D

R

Y

L

L

L

E

S

K

K

A

K

L

L

D

N

C

-

P

K

V

V

R

P

L

L

A

I

K

N

D

D

Y

W

L

L

D

K

G

G

V

V

E

A

A

V

N

E

V

P

G

G

E

Q

V

A

V

I

V

L

F

C

E

E

N

N

V

V

R

R

F

F

N

N

V

K

G

G

E

E

K

N

K

E

N

N

D

N

E

T

A

E

L

L

A

A

K

K

K

R

L

M

A

A

A

E

L

L

C

C

D

D

V

I

Y

F

V

V

M

M

D

D

A

A

F

F

G

A

T

T

A

A

H

H

R

R

A

A

Q

Q

A

A

S

S

T

T

H

A

G

G

V

V

G

A

L

A

Y

Y

A

A

P

K

V

L

A

A

C

C

A

A

G

G

P

P

L

L

L

L

A

I

G

S

E

E

L

V

E

E

A

A

L

L

G

S

K

R

A

A

M

L

D

E

N

N

P

P

A

Q

R

K

P

P

L

L

V

V

A

A

I

V

V

V

G
|
G

G
|
G

S
|
S

K
|
K

V

V

S

S

T

T

K
|
K

L

I

T

H

V

L

L

L

E

E

S

N

L

L

S

L

G

D

I

K

V

V

D

D

Q

Q

L

L

V

I

V

V

G

G

G
|
G

G
|
G

I

I

A

A

N
|
N

T

T

F

F

I

L

A

K

A

A

A

Q

G

G

H

Y

N

S

V

I

G

G

K

K

S

S

L
|
L

Y

C

E

E

A

N

D

E

L

W

I

L

D

D

E

A

A

A

K

Q

R

Q

L

F

V

W

A

E

N

K

A

A

Q

A

S

E

R

K

G

N

G

V

D

S

I

L

P

P

V

L

P

P

T

V

D

D

V

V

V

I

V

V

A

A

S

D

E

E

F

L

S

S

P

E

T

D

A

A

S

K

A

A

T

T

L

V

K

K

A

N

V

I

D

D

Q

A

V

V

A

T

D

S

E

N

D

E

M

S

I
|
I

F
|
F

D
|
D

I

V

G

G

P

P

D

N

S

T

A

S

E

A

A

T

L

Y

A

A

E

K

I

L

L

M

K

A

N

Q

A

A

G

G

T

T

I

I

V

V

W

W

N
|
N

G
|
G

P
|
P

V

I

G
|
G

V
|
V

F

F

E
|
E

F

I

D

E

Q

A

F
|
F

G
x
S

E

Q

G
|
G

T
|
T

K

R

R

A

I

L

A

A

Q

Q

A

A

I

V

A

A

D

K

S

S

S

T

A

A

F

Y

S

S

I

I

A

V

G

G

G
|
G

G
|
G

D
|
D

T
|
T

L

L

A

A

A

A

V

L

D

D

K

K

Y

F

D

N

I

L

A

T

D

D

K

Q

V

M

S

S

Y

Y

I

V

S

S

T

T

G

A

G

G

G
|
G

A

A

F

F

L

L

E

E

F

F

L

L

E

E

G

G

K

K

E

I

L

L

P

P

A

A

V

I

A

K

M

I

L

L

E

T

S

Q

R

R

G

A

E

K

active site: R35 (= R36), K191 (= K193), G344 (= G346), G367 (= G369)
binding adenosine-5'-diphosphate: G188 (= G190), G189 (= G191), S190 (= S192), K195 (= K197), G213 (= G215), G214 (= G216), N217 (= N219), L231 (= L233), I286 (= I288), F287 (= F289), N310 (= N312), G311 (= G313), P312 (= P314), G314 (= G316), V315 (= V317), E317 (= E319), G343 (= G345), G344 (= G346), D345 (= D347), T346 (= T348)
binding magnesium ion: D288 (= D290), G314 (= G316), F321 (= F323), S322 (≠ G324), G324 (= G326), T325 (= T327)

P40924 Phosphoglycerate kinase; EC 2.7.2.3 from Bacillus subtilis (strain 168) (see paper)
48% identity, 98% coverage: 5:389/391 of query aligns to 5:394/394 of P40924

query
sites
P40924

N

T

M

L

S

K

A

D

L

I

D

D

L

V

Q

K

G

G

K

K

R

V

V

V

L

F

I

C

R

R

E

V

D

D

L

F

N

N

V

V

P

P

V

M

S

K

N

D

G

G

V

E

V

V

T

T

S

D

D

D

A

T

R

R

L

I

R

R

A

A

S

A

L

L

P

P

T

T

I

I

K

K

L

H

A

L

L

A

E

D

K

Q

G

G

A

A

A

K

V

V

M

L

V

L

M

A

S

S

H

H

L

L

G

G

R

R

P

P

T

-

E

K

G

G

E

E

Y

V

N

V

S

E

E

E

F

L

S

R

M

L

Q

T

P

P

V

V

V

A

D

A

Y

R

L

L

E

G

K

E

A

L

L

L

D

G

C

K

P

E

V

V

R

K

L

K

A

A

K

D

D

E

-

A

Y

Y

L

G

D

D

G

A

V

V

E

K

A

A

N

Q

V

I

-

S

-

E

-

M

-

K

-

D

G

G

E

D

V

V

V

L

V

V

F

L

E

E

N

N

V

V

R

R

F

F

N

Y

V

P

G

G

E

E

K

E

K

K

N

N

D

D

E

P

A

E

L

L

A

A

K

K

K

A

L

F

A

A

A

E

L

L

C

A

D

D

V

V

Y

Y

V

V

M

N

D

D

A

A

F

F

G

G

T

A

A

A

H

H

R

R

A

A

Q

H

A

A

S

S

T

T

H

A

G

G

V

I

G

A

L

E

Y

H

A

L

P

P

V

-

A

A

C

V

A

A

G

G

P

F

L

L

L

M

A

E

G

K

E

E

L

L

E

D

A

V

L

L

G

G

K

K

A

A

M

V

D
x
S

N

N

P

P

A

D

R

R

P

P

L

F

V

T

A

A

I

I

V

I

G

G

G

G

S

A

K

K

V

V

S

K

T

D

K

K

L

I

T

G

V

V

L

I

E

E

S

S

L

L

S

L

G

D

I

K

V

V

D

D

Q

N

L

L

V

I

V

I

G

G

G

G

G

G

I

L

A

A

N

Y

T

T

F

F

I

V

A

K

A

A

A

L

G

G

H

Y

N

E

V

V

G

G

K

K

S

S

L

L

Y

L

E

E

A

E

D

D

L

K

I

I

D

E

E

L

A

A

K

K

R

S

L

F

V

M

A

D

N

R

A

A

Q

K

S

E

R

K

G

G

G

V

D

N

I

F

P

Y

V

M

P

P

T

E

D

D

V

V

V

L

V

V

A

A

S

D

E

D

F

F

S

S

P

N

T

D

A

A

S

N

A

V

T

K

L

I

K

V

A

P

V

I

D

S

Q

E

V

I

A

P

D

S

E

D

D

L

M

E

I

A

F

I

D

D

I

I

G

G

P

T

D

K

S
x
T

A

R

E

E

A

T

L

Y

A

A

E

D

I

V

L

I

K

K

N

N

A

S

G

K

T

L

I

V

V

V

W

W

N

N

G

G

P

P

V

M

G

G

V

V

F

F

E

E

F

I

D

D

Q

L

F

F

G

A

E

Q

G

G

T

T

K

K

R

A

I

V

A

A

Q

E

A

A

I

L

A

A

D

E

S

A

S

K

-

D

A

T

F

Y

S

S

I

V

A

I

G

G

G

G

G

G

D

D

T

S

L

A

A

A

A

A

V

V

D

E

K

K

Y

F

D

G

I

L

A

A

D

D

K

K

V

M

S

S

Y

H

I

I

S

S

T

T

G

G

G

G

G

G

A

A

F

S

L

L

E

E

F

F

L

M

E

E

G

G

K

K

E

E

L

L

P

P

A

G

V

V

A

A

M

A

L

L

E

N

S

D

R

K

S183 (≠ D179) modified: Phosphoserine
T299 (≠ S295) modified: Phosphothreonine

1phpA Structure of the adp complex of the 3-phosphoglycerate kinase from bacillus stearothermophilus at 1.65 angstroms (see paper)
47% identity, 97% coverage: 9:389/391 of query aligns to 9:394/394 of 1phpA

query
sites
1phpA

L

V

D

D

L

V

Q

R

G

G

K

K

R

R

V

V

L

F

I

C

R

R

E

V

D

D

L

F

N

N

V

V

P

P

V

M

S

E

N

Q

G

G

V

A

V

I

T

T

S

D

D

D

A

T

R
|
R

L

I

R

R

A

A

S

A

L

L

P

P

T

T

I

I

K

R

L

Y

A

L

L

I

E

E

K

H

G

G

A

A

A

K

V

V

M

I

V

L

M

A

S

S

H

H

L

L

G

G

R

R

P

P

T

-

E

K

G

G

E

K

Y

V

N

V

S

E

E

E

F

L

S

R

M

L

Q

D

P

A

V

V

V

A

D

K

Y

R

L

L

E

G

K

E

A

L

L

L

D

E

C

R

P

P

V

V

R

A

L

K

A

T

K

N

D

E

Y

A

L

V

-

G

D

D

G

E

V

V

E

K

A

A

-

A

-

V

-

D

-

R

-

L

N

N

V

E

G

G

E

D

V

V

V

L

V

L

F

L

E

E

N

N

V

V

R

R

F

F

N

Y

V

P

G

G

E

E

K

E

K

K

N

N

D

D

E

P

A

E

L

L

A

A

K

K

K

A

L

F

A

A

A

E

L

L

C

A

D

D

V

L

Y

Y

V

V

M

N

D

D

A

A

F

F

G

G

T

A

A

A

H

H

R

R

A

A

Q

H

A

A

S

S

T

T

H

E

G

G

V

I

G

A

L

H

Y

Y

A

L

P

P

V

-

A

A

C

V

A

A

G

G

P

F

L

L

L

M

A

E

G

K

E

E

L

L

E

E

A

V

L

L

G

G

K

K

A

A

M

L

D

S

N

N

P

P

A

D

R

R

P

P

L

F

V

T

A

A

I

I

V

I

G

G

G
|
G

S
x
A

K
|
K

V

V

S

K

T

D

K
|
K

L

I

T

G

V

V

L

I

E

D

S

N

L

L

S

L

G

E

I

K

V

V

D

D

Q

N

L

L

V

I

V

I

G

G

G
|
G

G
|
G

I

L

A

A

N

Y

T

T

F

F

I

V

A

K

A

A

A

L

G

G

H

H

N

D

V

V

G

G

K

K

S

S

L
|
L

Y

L

E

E

A

E

D

D

L

K

I

I

D

E

E

L

A

A

K

K

R

S

L

F

V

M

A

E

N

K

A

A

Q

K

S

E

R

K

G

G

G

V

D

R

I

F

P

Y

V

M

P

P

T

V

D

D

V

V

V

V

V

V

A

A

S

D

E

R

F

F

S

A

P

N

T

D

A

A

S

N

A

T

T

K

L

V

K

V

A

P

V

I

D

D

Q

A

V

I

A

P

D

A

E

D

D

W

M

S

I
x
A

F
x
L

D

D

I

I

G

G

P

P

D

K

S

T

A

R

E

E

A

L

L

Y

A

R

E

D

I

V

L

I

K

R

N

E

A

S

G

K

T

L

I

V

V

V

W

W

N
|
N

G
|
G

P
|
P

V
x
M

G
|
G

V
|
V

F

F

E
|
E

F

M

D

D

Q

A

F

F

G

A

E

H

G

G

T

T

K

K

-

A

R

I

I

A

A

E

Q

A

A

L

I

A

A

E

D

A

S

L

S

D

A

T

F

Y

S

S

I

V

A

I

G

G

G
|
G

G
|
G

D
|
D

T
x
S

L

A

A

A

A

A

V

V

D

E

K

K

Y

F

D

G

I

L

A

A

D

D

K

K

V

M

S

D

Y

H

I

I

S

S

T

T

G

G

G

G

G
|
G

A

A

F

S

L

L

E

E

F

F

L

M

E

E

G

G

K

K

E

Q

L

L

P

P

A

G

V

V

A

V

M

A

L

L

E

E

S

D

R

K

active site: R36 (= R36), K197 (= K193), G351 (= G346), G374 (= G369)
binding adenosine-5'-diphosphate: G195 (= G191), A196 (≠ S192), K201 (= K197), G219 (= G215), G220 (= G216), L237 (= L233), A292 (≠ I288), L293 (≠ F289), N316 (= N312), G317 (= G313), P318 (= P314), M319 (≠ V315), G320 (= G316), V321 (= V317), E323 (= E319), G350 (= G345), G351 (= G346), D352 (= D347), S353 (≠ T348)

P18912 Phosphoglycerate kinase; EC 2.7.2.3 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
47% identity, 97% coverage: 9:389/391 of query aligns to 9:394/394 of P18912

query
sites
P18912

L

V

D

D

L

V

Q

R

G

G

K

K

R

R

V

V

L

F

I

C

R

R

E

V

D

D

L

F

N

N

V

V

P

P

V

M

S

E

N

Q

G

G

V

A

V

I

T

T

S

D

D

D

A

T

R

R

L

I

R

R

A

A

S

A

L

L

P

P

T

T

I

I

K

R

L

Y

A

L

L

I

E

E

K

H

G

G

A

A

A

K

V

V

M

I

V

L

M

A

S

S

H

H

L

L

G

G

R

R

P

P

T

-

E

K

G

G

E

K

Y

V

N

V

S

E

E

E

F

L

S

R

M

L

Q

D

P

A

V

V

V

A

D

K

Y

R

L

L

E

G

K

E

A

L

L

L

D

E

C

R

P

P

V

V

R

A

L

K

A

T

K

N

D

E

Y

A

L

V

-

G

D

D

G

E

V

V

E

K

A

A

-

A

-

V

-

D

-

R

-

L

N

N

V

E

G

G

E

D

V

V

V

L

V

L

F

L

E

E

N

N

V

V

R

R

F

F

N

Y

V

P

G

G

E

E

K

E

K

K

N

N

D

D

E

P

A

E

L

L

A

A

K

K

K

A

L

F

A

A

A

E

L

L

C

A

D

D

V

L

Y

Y

V

V

M

N

D

D

A

A

F

F

G

G

T

A

A

A

H

H

R

R

A

A

Q

H

A

A

S

S

T

T

H

E

G

G

V

I

G

A

L

H

Y

Y

A

L

P

P

V

-

A

A

C

V

A

A

G

G

P

F

L

L

L

M

A

E

G

K

E

E

L

L

E

E

A

V

L

L

G

G

K

K

A

A

M

L

D

S

N

N

P

P

A

D

R

R

P

P

L

F

V

T

A

A

I

I

V

I

G

G

G

G

S

A

K

K

V

V

S

K

T

D

K
|
K

L

I

T

G

V

V

L

I

E

D

S

N

L

L

S

L

G

E

I

K

V

V

D

D

Q

N

L

L

V

I

V

I

G

G

G

G

G

G

I

L

A

A

N

Y

T

T

F

F

I

V

A

K

A

A

A

L

G

G

H

H

N

D

V

V

G

G

K

K

S

S

L

L

Y

L

E

E

A

E

D

D

L

K

I

I

D

E

E

L

A

A

K

K

R

S

L

F

V

M

A

E

N

K

A

A

Q

K

S

E

R

K

G

G

G

V

D

R

I

F

P

Y

V

M

P

P

T

V

D

D

V

V

V

V

V

V

A

A

S

D

E

R

F

F

S

A

P

N

T

D

A

A

S

N

A

T

T

K

L

V

K

V

A

P

V

I

D

D

Q

A

V

I

A

P

D

A

E

D

D

W

M

S

I

A

F

L

D

D

I

I

G

G

P

P

D

K

S

T

A

R

E

E

A

L

L

Y

A

R

E

D

I

V

L

I

K

R

N

E

A

S

G

K

T

L

I

V

V

V

W

W

N
|
N

G

G

P

P

V

M

G

G

V

V

F

F

E
|
E

F

M

D

D

Q

A

F

F

G

A

E

H

G

G

T

T

K

K

-

A

R

I

I

A

A

E

Q

A

A

L

I

A

A

E

D

A

S

L

S

D

A

T

F

Y

S

S

I

V

A

I

G

G

G
|
G

G
|
G

D
|
D

T
x
S

L

A

A

A

A

A

V

V

D

E

K

K

Y

F

D

G

I

L

A

A

D

D

K

K

V

M

S

D

Y

H

I

I

S

S

T

T

G

G

G

G

G

G

A

A

F

S

L

L

E

E

F

F

L

M

E

E

G

G

K

K

E

Q

L

L

P

P

A

G

V

V

A

V

M

A

L

L

E

E

S

D

R

K

K201 (= K197) binding
N316 (= N312) binding
E323 (= E319) binding
GGDS 350:353 (≠ GGDT 345:348) binding

1vpeA Crystallographic analysis of phosphoglycerate kinase from the hyperthermophilic bacterium thermotoga maritima (see paper)
46% identity, 99% coverage: 4:389/391 of query aligns to 3:396/398 of 1vpeA

query
sites
1vpeA

I

M

N

T

M

I

S

R

A

D

L

V

D

D

L

L

Q

K

G

G

K

K

R

R

V

V

L

I

I

M

R

R

E

V

D

D

L

F

N

N

V

V

P

P

V

V

S

K

N

D

G

G

V

V

V

V

T

Q

S

D

D

D

A

T

R
|
R

L

I

R

R

A

A

S

A

L

L

P

P

T

T

I

I

K

K

L

Y

A

A

L

L

E

E

K

Q

G

G

A

A

A

K

V

V

M

I

V

L

M

L

S

S

H

H

L

L

G

G

R

R

P

P

T

-

E

K

G

G

E

E

Y

P

N

S

S

P

E

E

F

F

S

S

M

L

Q

A

P

P

V

V

V

A

D

K

Y

R

L

L

E

S

K

E

A

L

L

L

D

G

C

K

P

E

V

V

R

K

L

F

A

V

K

P

D

A

Y

V

L

V

-

G

D

D

G

E

V

V

E

K

A

K

N

A

V

V

-

E

-

E

-

L

-

K

-

E

G

G

E

E

V

V

V

L

V

L

F

L

E

E

N

N

V

T

R

R

F

F

N

H

V

P

G

G

E

E

K

T

K

K

N

N

D

D

E

P

A

E

L

L

A

A

K

K

K

F

L

W

A

A

A

S

L

L

C

A

D

D

V

I

Y

H

V

V

M

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

Q

H

A

A

S

S

T

N

H

V

G

G

V

I

G

A

L

Q

Y

F

A

I

P

P

V

-

A

S

C

V

A

A

G

G

P

F

L

L

L

M

A

E

G

K

E

E

L

I

E

K

A

F

L

L

G

S

K

K

A

V

M

T

D

Y

N

N

P

P

A

E

R

K

P

P

L

Y

V

V

A

V

I

V

V

L

G

G

G
|
G

S
x
A

K
|
K

V

V

S

S

T

D

K
|
K

L

I

T

G

V

V

L

I

E

T

S

N

L

L

S

M

G

E

I

K

V

A

D

D

Q

R

L

I

V

L

V

I

G

G

G
|
G

G
x
A

I

M

A

M

N

F

T

T

F

F

I

L

A

K

A

A

A

L

G

G

H

K

N

E

V

V

G

G

K

S

S

S

L

R

Y

V

E

E

A

E

D

D

L

K

I

I

D

D

E

L

A

A

K

K

R

E

L

L

V

V

A

E

N

K

A

A

Q

K

S

E

R

K

G

G

G

V

D

E

I

I

P

V

V

L

P

P

T

V

D

D

V

A

V

V

V

I

A

A

S

Q

E

K

F

I

S

E

P

P

T

G

A

V

S

E

A

K

T

K

L

V

K

V

A

R

V

I

D

D

Q

D

-

G

V

I

A

P

D

E

E

G

D

W

M

M

I
x
G

F
x
L

D

D

I

I

G

G

P

P

D

E

S

T

A

I

E

E

A

L

L

F

A

K

E

Q

I

K

L

L

K

S

N

D

A

A

G

K

T

T

I

V

V

V

W

W

N
|
N

G
|
G

P
|
P

V
x
M

G
|
G

V
|
V

F

F

E
|
E

F

I

D

D

Q

D

F

F

G

A

E

E

G

G

T

T

K

K

R

Q

I

V

A

A

Q

L

A

A

I

I

A

A

-

A

-

L

-

T

D

E

S

K

S

G

A

A

F

I

S

T

I

V

A

V

G

G

G
|
G

G
|
G

D
|
D

T
x
S

L

A

A

A

A

A

V

V

D

N

K

K

Y

F

D

G

I

L

A

E

D

D

K

K

V

F

S

S

Y

H

I

V

S

S

T

T

G

G

G
|
G

G
|
G

A

A

F

S

L

L

E

E

F

F

L

L

E

E

G

G

K

K

E

E

L

L

P

P

A

G

V

I

A

A

M

S

L

M

E

R

S

I

R

K

active site: R35 (= R36), K196 (= K193), G353 (= G346), G376 (= G369)
binding phosphoaminophosphonic acid-adenylate ester: G194 (= G191), A195 (≠ S192), K196 (= K193), K200 (= K197), G218 (= G215), A219 (≠ G216), G292 (≠ I288), L293 (≠ F289), N316 (= N312), G317 (= G313), P318 (= P314), M319 (≠ V315), G320 (= G316), V321 (= V317), E323 (= E319), G352 (= G345), G353 (= G346), D354 (= D347), S355 (≠ T348), G375 (= G368)

P36204 Bifunctional PGK/TIM; EC 2.7.2.3; EC 5.3.1.1 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
46% identity, 98% coverage: 1:384/391 of query aligns to 1:392/654 of P36204

query
sites
P36204

M

M

A

E

I

K

I

M

N

T

M

I

S

R

A

D

L

V

D

D

L

L

Q

K

G

G

K

K

R

R

V

V

L

I

I

M

R

R

E

V

D

D

L

F

N

N

V

V

P

P

V

V

S

K

N

D

G

G

V

V

V

V

T

Q

S

D

D

D

A

T

R
|
R

L

I

R

R

A

A

S

A

L

L

P

P

T

T

I

I

K

K

L

Y

A

A

L

L

E

E

K

Q

G

G

A

A

A

K

V

V

M

I

V

L

M

L

S

S

H

H

L

L

G

G

R

R

P

P

T

-

E

K

G

G

E

E

Y

P

N

S

S

P

E

E

F

F

S

S

M

L

Q

A

P

P

V

V

V

A

D

K

Y

R

L

L

E

S

K

E

A

L

L

L

D

G

C

K

P

E

V

V

R

K

L

F

A

V

K

P

D

A

Y

V

L

V

-

G

D

D

G

E

V

V

E

K

A

K

N

A

V

V

-

E

-

E

-

L

-

K

-

E

G

G

E

E

V

V

V

L

V

L

F

L

E

E

N

N

V

T

R
|
R

F

F

N

H

V

P

G

G

E

E

K

T

K

K

N

N

D

D

E

P

A

E

L

L

A

A

K

K

K

F

L

W

A

A

A

S

L

L

C

A

D

D

V

I

Y

H

V

V

M

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R
|
R

A

A

Q

H

A

A

S

S

T

N

H

V

G

G

V

I

G

A

L

Q

Y

F

A

I

P

P

V

-

A

S

C

V

A

A

G

G

P

F

L

L

L

M

A

E

G

K

E

E

L

I

E

K

A

F

L

L

G

S

K

K

A

V

M

T

D

Y

N

N

P

P

A

E

R

K

P

P

L

Y

V

V

A

V

I

V

V

L

G

G

G

G

S

A

K

K

V

V

S

S

T

D

K

K

L

I

T

G

V

V

L

I

E

T

S

N

L

L

S

M

G

E

I

K

V

A

D

D

Q

R

L

I

V

L

V

I

G

G

G

G

G

A

I

M

A

M

N

F

T

T

F

F

I

L

A

K

A

A

A

L

G

G

H

K

N

E

V

V

G

G

K

S

S

S

L

R

Y

V

E

E

A

E

D

D

L

K

I

I

D

D

E

L

A

A

K

K

R

E

L

L

V

L

A

E

N

K

A

A

Q

K

S

E

R

K

G

G

G

V

D

E

I

I

P

V

V

L

P

P

T

V

D

D

V

A

V

V

V

I

A

A

S

Q

E

K

F

I

S

E

P

P

T

G

A

V

S

E

A

K

T

K

L

V

K

V

A

R

V

I

D

D

Q

D

-

G

V

I

A

P

D

E

E

G

D

W

M

M

I

G

F

L

D

D

I

I

G

G

P

P

D

E

S

T

A

I

E

E

A

L

L

F

A

K

E

Q

I

K

L

L

K

S

N

D

A

A

G

K

T

T

I

V

V

V

W

W

N

N

G

G

P

P

V

M

G

G

V

V

F

F

E

E

F

I

D

D

Q

D

F

F

G

A

E

E

G

G

T

T

K

K

R

Q

I

V

A

A

Q

L

A

A

I

I

A

A

-

A

-

L

-

T

D

E

S

K

S

G

A

A

F

I

S

T

I

V

A

V

G

G

G

G

G

G

D

D

T

S

L

A

A

A

A

A

V

V

D

N

K

K

Y

F

D

G

I

L

A

E

D

D

K

K

V

F

S

S

Y

H

I

V

S

S

T

T

G

G

G

G

G

G

A

A

F

S

L

L

E

E

F

F

L

L

E

E

G

G

K

K

E

E

L

L

P

P

A

G

V

I

A

A

R36 (= R36) binding
R118 (= R113) binding
R151 (= R146) binding

16pkA Phosphoglycerate kinase from trypanosoma brucei bisubstrate analog (see paper)
39% identity, 98% coverage: 5:389/391 of query aligns to 4:415/415 of 16pkA

query
sites
16pkA

N

S

M

I

S

N

A

E

L

C

D

D

L

L

Q

K

G

G

K

K

R

K

V

V

L

L

I

I

R

R

E

V

D

D

L

F

N

N

V

V

P

P

V

V

S

K

N

N

G

G

V

K

V

I

T

T

S

N

D

D

A

Y

R
|
R

L

I

R

R

A

S

S

A

L

L

P

P

T

T

I

L

K

K

L

K

A

V

L

L

E

T

K

E

G

G

A

G

A

S

V

C

M

V

V

L

M

M

S

S

H

H

L

L

G

G

R

R

P

P

-

K

-

G

-

I

-

P

-

M

-

A

-

Q

-

A

-

G

-

K

-

I

-

R

-

S

T

T

E

G

G

G

-

V

-

P

E

G

Y

F

N

Q

S

Q

E

K

F

A

S

T

M

L

Q

K

P

P

V

V

V

A

D

K

Y

R

L

L

E

S

K

E

A

L

L

L

D

L

C

R

P

P

V

V

R

T

L

F

A

A

K

P

D

D

Y

C

L

L

D

N

G

A

V

A

E

D

A

V

-

V

-

S

-

K

-

M

N

S

V

P

G

G

E

D

V

V

V

V

V

L

F

L

E

E

N

N

V

V

R

R

F

F

N

Y

V

K

G

E

E

E

-

G

-

S

-

K

-

K

K

A

K

K

N

D

D

R

E

E

A

A

L

M

A

A

K

K

K

I

L

L

A

A

A

S

L

Y

C

G

D

D

V

V

Y

Y

V

I

M

S

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

D

Q

S

A

A

S

T

T

M

H

T

G

G

V

I

G

P

L

K

Y

I

A

L

P

G

V

N

A

G

C

A

A

A

G

G

P

Y

L

L

L

M

A

E

G

K

E

E

L

I

E

S

A

Y

L

F

G

A

K

K

A

V

M

L

D

G

N

N

P

P

A

P

R

R

P

P

L

L

V

V

A

A

I

I

V

V

G

G

G
|
G

S
x
A

K
|
K

V

V

S

S

T

D

K
|
K

L

I

T

Q

V

L

L

L

E

D

S

N

L

M

S

L

G

Q

I

R

V

I

D

D

Q

Y

L

L

V

L

V

I

G

G

G
|
G

G
x
A

I

M

A

A

N
x
Y

T

T

F

F

I

L

A

K

A

A

A

Q

G

G

H

Y

N

S

V

I

G

G

K

K

S

S

L
x
K

Y

C

E

E

A

E

D

S

L

K

I

L

D

E

E

F

A

A

K

R

R

S

L

L

V

L

A

K

N

K

A

A

Q

E

S

D

R

R

G

K

G

V

D

Q

I

V

P

I

V

L

P

P

T

I

D

D

V

H

V

V

V

C

A

H

S

T

E

E

F

F

S

K

P

A

T

V

A

D

S

S

A

P

T

L

L

I

K

T

A

E

V

D

D

Q

Q

N

V

I

A

P

D

E

E

G

D

H

M

M

I
x
A

F
x
L

D

D

I

I

G

G

P

P

D

K

S

T

A

I

E

E

A

K

L

Y

A

V

E

Q

I

T

L

I

K

G

N

K

A

C

G

K

T

S

I

A

V

I

W

W

N

N

G
|
G

P
|
P

V

M

G
|
G

V
|
V

F

F

E
|
E

F

M

D

V

Q

P

F

Y

G

S

E

K

G

G

T

T

K

F

R

A

I

I

A

A

Q

K

A

A

I

M

A

G

-

R

-

G

-

T

-

H

D

E

S

H

S

G

A

L

F

M

S

S

I

I

A

I

G

G

G

G

G
|
G

D

D

T

S

L

A

A

S

A

A

V

A

D

E

K

L

Y

S

D

G

I

E

A

A

D

K

K

R

V

M

S

S

Y

H

I

V

S

S

T

T

G
|
G

G
|
G

G
|
G

A

A

F

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

T

L

L

P

P

A

G

V

V

A

T

M

V

L

L

E

D

S

D

R

K

active site: R35 (= R36), K215 (= K193), G372 (= G346), G395 (= G369)
binding 1,1,5,5-tetrafluorophosphopentylphosphonic acid adenylate ester: G213 (= G191), A214 (≠ S192), K219 (= K197), G237 (= G215), A238 (≠ G216), Y241 (≠ N219), K255 (≠ L233), A310 (≠ I288), L311 (≠ F289), G335 (= G313), P336 (= P314), G338 (= G316), V339 (= V317), E341 (= E319), G372 (= G346), G393 (= G367), G394 (= G368), G395 (= G369)

13pkA Ternary complex of phosphoglycerate kinase from trypanosoma brucei (see paper)
39% identity, 98% coverage: 5:389/391 of query aligns to 4:415/415 of 13pkA

query
sites
13pkA

N

S

M

I

S

N

A

E

L

C

D

D

L

L

Q

K

G

G

K

K

R

K

V

V

L

L

I

I

R

R

E

V

D

D

L

F

N

N

V

V

P

P

V

V

S

K

N

N

G

G

V

K

V

I

T

T

S

N

D

D

A

Y

R
|
R

L

I

R

R

A

S

S

A

L

L

P

P

T

T

I

L

K

K

L

K

A

V

L

L

E

T

K

E

G

G

A

G

A

S

V

C

M

V

V

L

M

M

S

S

H

H

L

L

G

G

R

R

P

P

-

K

-

G

-

I

-

P

-

M

-

A

-

Q

-

A

-

G

-

K

-

I

-

R

-

S

T

T

E

G

G

G

-

V

-

P

E

G

Y

F

N

Q

S

Q

E

K

F

A

S

T

M

L

Q

K

P

P

V

V

V

A

D

K

Y

R

L

L

E

S

K

E

A

L

L

L

D

L

C

R

P

P

V

V

R

T

L

F

A

A

K

P

D

D

Y

C

L

L

D

N

G

A

V

A

E

D

A

V

-

V

-

S

-

K

-

M

N

S

V

P

G

G

E

D

V

V

V

V

V

L

F

L

E

E

N

N

V

V

R

R

F

F

N

Y

V

K

G

E

E

E

-

G

-

S

-

K

-

K

K

A

K

K

N

D

D

R

E

E

A

A

L

M

A

A

K

K

K

I

L

L

A

A

A

S

L

Y

C

G

D

D

V

V

Y

Y

V

I

M

S

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

D

Q

S

A

A

S

T

T

M

H

T

G

G

V

I

G

P

L

K

Y

I

A

L

P

G

V

N

A

G

C

A

A

A

G

G

P

Y

L

L

L

M

A

E

G

K

E

E

L

I

E

S

A

Y

L

F

G

A

K

K

A

V

M

L

D

G

N

N

P

P

A

P

R

R

P

P

L

L

V

V

A

A

I

I

V

V

G

G

G
|
G

S
x
A

K
|
K

V

V

S

S

T

D

K
|
K

L

I

T

Q

V

L

L

L

E

D

S

N

L

M

S

L

G

Q

I

R

V

I

D

D

Q

Y

L

L

V

L

V

I

G

G

G
|
G

G
x
A

I

M

A

A

N
x
Y

T

T

F

F

I

L

A

K

A

A

A

Q

G

G

H

Y

N

S

V

I

G

G

K

K

S

S

L

K

Y

C

E

E

A

E

D

S

L

K

I

L

D

E

E

F

A

A

K

R

R

S

L

L

V

L

A

K

N

K

A

A

Q

E

S

D

R

R

G

K

G

V

D

Q

I

V

P

I

V

L

P

P

T

I

D

D

V

H

V

V

V

C

A

H

S

T

E

E

F

F

S

K

P

A

T

V

A

D

S

S

A

P

T

L

L

I

K

T

A

E

V

D

D

Q

Q

N

V

I

A

P

D

E

E

G

D

H

M

M

I
x
A

F
x
L

D

D

I

I

G

G

P

P

D

K

S

T

A

I

E

E

A

K

L

Y

A

V

E

Q

I

T

L

I

K

G

N

K

A

C

G

K

T

S

I

A

V

I

W

W

N
|
N

G
|
G

P
|
P

V

M

G
|
G

V
|
V

F

F

E
|
E

F

M

D

V

Q

P

F

Y

G

S

E

K

G

G

T

T

K

F

R

A

I

I

A

A

Q

K

A

A

I

M

A

G

-

R

-

G

-

T

-

H

D

E

S

H

S

G

A

L

F

M

S

S

I

I

A

I

G

G

G
|
G

G
|
G

D
|
D

T
x
S

L

A

A

S

A

A

V

A

D

E

K

L

Y

S

D

G

I

E

A

A

D

K

K

R

V

M

S

S

Y

H

I

V

S

S

T

T

G

G

G

G

G
|
G

A

A

F

S

L

L

E

E

F

L

L

L

E

E

G

G

K

K

E

T

L

L

P

P

A

G

V

V

A

T

M

V

L

L

E

D

S

D

R

K

active site: R35 (= R36), K215 (= K193), G372 (= G346), G395 (= G369)
binding adenosine-5'-diphosphate: G213 (= G191), A214 (≠ S192), K215 (= K193), K219 (= K197), G237 (= G215), A238 (≠ G216), Y241 (≠ N219), A310 (≠ I288), L311 (≠ F289), N334 (= N312), G335 (= G313), P336 (= P314), G338 (= G316), V339 (= V317), E341 (= E319), G371 (= G345), G372 (= G346), D373 (= D347), S374 (≠ T348)

P07378 Phosphoglycerate kinase, glycosomal; Phosphoglycerate kinase C; EC 2.7.2.3 from Trypanosoma brucei brucei (see 2 papers)
39% identity, 98% coverage: 5:389/391 of query aligns to 8:419/440 of P07378

query
sites
P07378

N

S

M

I

S

N

A

E

L

C

D

D

L

L

Q

K

G

G

K

K

R

K

V

V

L

L

I

I

R

R

E

V

D
|
D

L
x
F

N
|
N

V

V

P

P

V

V

S

K

N

N

G

G

V

K

V

I

T

T

S

N

D

D

A

Y

R
|
R

L

I

R

R

A

S

S

A

L

L

P

P

T

T

I

L

K

K

L

K

A

V

L

L

E

T

K

E

G

G

A

G

A

S

V

C

M

V

V

L

M

M

S

S

H
|
H

L
|
L

G
|
G

R
|
R

P

P

-

K

-

G

-

I

-

P

-

M

-

A

-

Q

-

A

-

G

-

K

-

I

-

R

-

S

T

T

E

G

G

G

-

V

-

P

E

G

Y

F

N

Q

S

Q

E

K

F

A

S

T

M

L

Q

K

P

P

V

V

V

A

D

K

Y

A

L

L

E

S

K

E

A

L

L

L

D

L

C

R

P

P

V

V

R

T

L

F

A

A

K

P

D

D

Y

C

L

L

D

N

G

A

V

A

E

D

A

V

-

V

-

S