SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 5208454 Shew_0966 beta alanine--pyruvate transaminase (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9A3Q9 Omega-aminotransferase; Beta-alanine--pyruvate aminotransferase; EC 2.6.1.-; EC 2.6.1.18 from Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus) (see paper)
59% identity, 100% coverage: 1:445/446 of query aligns to 1:438/439 of Q9A3Q9

query
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Q9A3Q9

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V227 (= V234) mutation to G: Decreases activity toward 3-aminobutanoate by 2-fold. Increases activity toward the aromatic beta-amino acid 3-amino-3-phenylpropanoate by 2-fold.
R260 (= R267) mutation to L: Decreases activity toward 3-aminobutanoate by 30-fold.
N285 (= N292) mutation to A: Decreases activity toward 3-aminobutanoate by 4-fold. Increases activity toward the aromatic beta-amino acid 3-amino-3-phenylpropanoate by 3-fold.

4uhmA Characterization of a novel transaminase from pseudomonas sp. Strain aac (see paper)
51% identity, 97% coverage: 14:445/446 of query aligns to 3:434/435 of 4uhmA

query
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4uhmA

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active site: F11 (= F22), Y140 (= Y151), E213 (= E226), D246 (= D259), I249 (= I262), K275 (= K288), Q408 (≠ A419)
binding magnesium ion: A91 (= A102), D99 (≠ N110)
binding pyridoxal-5'-phosphate: S106 (= S117), G107 (= G118), S108 (= S119), Y140 (= Y151), H141 (= H152), G142 (= G153), E213 (= E226), S218 (= S231), D246 (= D259), V248 (= V261), I249 (= I262), K275 (= K288)

4b9bA The structure of the omega aminotransferase from pseudomonas aeruginosa (see paper)
49% identity, 97% coverage: 14:445/446 of query aligns to 4:435/436 of 4b9bA

query
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4b9bA

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F

F

G

E

I

A

F

G

E

M

T

K

C

L

F

W

K

Q

N

Q

G

G

T

F

L

Y

V

V

R

R

A

F

T

G

A

G

D

D

T

T

I

L

A
x
Q

L

F

S

G

P

P

P

T

L

F

I

N

V

A

D

R

E

P

A

E

Q

E

I

L

D

D

Q

R

M

L

V

F

N

D

T

A

L

V

S

G

D

E

A

A

I

L

T

N

T

G

V

I

active site: F12 (= F22), Y141 (= Y151), E214 (= E226), D247 (= D259), I250 (= I262), K276 (= K288), Q409 (≠ A419)
binding calcium ion: D168 (= D180), H169 (= H181)
binding pyridoxal-5'-phosphate: S107 (= S117), G108 (= G118), S109 (= S119), Y141 (= Y151), H142 (= H152), G143 (= G153), E214 (= E226), D247 (= D259), V249 (= V261), I250 (= I262), K276 (= K288), Y314 (= Y324), T315 (= T325)

Q9I700 Beta-alanine--pyruvate aminotransferase; Beta-A--Py AT; Beta-alanine--pyruvate transaminase; Omega-amino acid aminotransferase; Omega-amino acid AT; Omega-amino acid--pyruvate aminotransferase; Omega-APT; EC 2.6.1.18 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
49% identity, 97% coverage: 14:445/446 of query aligns to 16:447/448 of Q9I700

query
sites
Q9I700

T

N

L

L

E

R

H

A

Y

H

W

W

M

M

P

P

F

F

T

S

A

A

N

N

R

R

Q

N

F

F

K

Q

Q

K

S

D

P

P

R

R

L

I

A

V

Q

A

A

A

E

E

G

G

M

S

Y

W

Y

L

K

T

D

D

V

D

N

K

G

G

R

R

P

K

V

V

L

Y

D

D

G

S

T

L

A

S

G

G

L

L

W
|
W

C

T

C

C

N

G

A

A

G

G

H

H

G

S

R

R

K

K

A

E

I

I

S

Q

E

E

A

A

V

V

S

A

K

R

Q

Q

I

L

H

G

E

T

M

L

D

D

Y

Y

A

S

P

P

S

G

F

F

Q

Q

M

Y

G

G

H

H

P

P

L

L

A

S

F

F

E

Q

L

L

A

A

E

E

R

K

L

I

A

A

A

G

I

L

A

L

P

P

K

G

G

E

I

L

N

N

K

H

V

V

F

F

T

T

N

G

S

S

G

G

S

S

E

E

S

C

V

A

D

D

T

T

A

S

L

I

K

K

I

M

A

A

L

R

N

A

Y

Y

H

W

R

R

A

L

R

K

G

G

E

Q

A

P

T

Q

R

K

T

T

R

K

F

L

I

I

G

G

R

R

E

A

L

R

G

G

Y

Y

H

H

G

G

V

V

G

N

F

V

G

A

G

G

I

T

S

S

V

L

G

G

I

I

G

G

N

N

R

R

R

K

T

M

F

F

S

G

Q

Q

Q

-

L

-

L

L

Q

M

G

D

V

V

D

D

H

H

L

L

P

P

H

H

T

T

L

L

D

Q

I

P

Q

G

Q

M

N

-

A

A

F

F

C

T

K

R

G

G

L

M

P

A

E

Q

T

T

-

G

G

G

A

V

E

E

K

L

A

A

E

N

V

E

L

L

E

L

Q

K

L

L

V

I

T

E

L

L

H

H

G

D

A

A

E

S

N

N

I

I

A

A

V

V

I

I

V

V

E

E

P

P

M

M

S

S

G

G

S

S

A

A

G

G

V

V

I

L

L

V

P

P

Q

V

G

G

Y

Y

L

L

K

Q

R

R

L

L

R

R

E

E

I

I

T

C

R

D

K

Q

Y

H

G

N

I

I

L

L

I

I

F

F

D

D

E

E

V

V

I

I

T

T

G

A

F

F

G

G

R

R

V

L

G

G

D

T

A

Y

F

S

A

G

S

A

Q

E

R

Y

W

F

G

G

V

V

T

T

P

P

D

D

M

L

I

M

T

N

T

V

A

A

K

K

A

Q

L

V

N

T

N

N

G

G

T

A

I

V

P

P

M

M

G

G

A

A

V

V

L

I

I

A

D

S

A

E

I

I

Y

Y

D

D

A

T

S

F

M

M

D

N

A

Q

-

A

-

L

P

P

E

E

G

H

A

A

I

V

E

E

L

F

Y

S

H

H

G

G

Y

Y

T
|
T

Y

Y

S

S

G

A

H

H

P

P

V

V

A

A

A

C

A

A

A

G

I

L

A

A

T

A

L

L

D

D

I

I

Y

L

Q

A

E

R

E

D

Q

N

L

L

F

V

E

Q

R

Q

T

S

K

A

S

E

L

L

E

A

G

P

Y

H

F

F

E

E

K

K

A

G

V

L

H

H

S

G

L

L

K

Q

G

G

L

A

P

K

N

N

L

V

I

I

D

D

I

I

R

R

N

N

T

C

G

G

L

L

V

A

A

G

G

A

I

I

Q

Q

F

I

S

A

P

P

S

R

D

D

K

G

G

D

I

P

G

T

K

V

R

R

G

P

F

F

G

E

I

A

F

G

E

M

T

K

C

L

F

W

K

Q

N

Q

G

G

T

F

L

Y

V

V

R
|
R

A

F

T

G

A

G

D

D

T

T

I

L

A
x
Q

L

F

S

G

P

P

P

T

L

F

I

N

V

A

D

R

E

P

A

E

Q

E

I

L

D

D

Q

R

M

L

V

F

N

D

T

A

L

V

S

G

D

E

A

A

I

L

T

N

T

G

V

I

W61 (= W59) binding
T327 (= T325) binding
R414 (= R412) binding
Q421 (≠ A419) binding

4b98A The structure of the omega aminotransferase from pseudomonas aeruginosa (see paper)
49% identity, 97% coverage: 14:445/446 of query aligns to 9:440/441 of 4b98A

query
sites
4b98A

T

N

L

L

E

R

H

A

Y

H

W

W

M

M

P

P

F
|
F

T

S

A

A

N

N

R

R

Q

N

F

F

K

Q

Q

K

S

D

P

P

R

R

L

I

A

V

Q

A

A

A

E

E

G

G

M

S

Y

W

Y

L

K

T

D

D

V

D

N

K

G

G

R

R

P

K

V

V

L

Y

D

D

G

S

T

L

A

S

G

G

L
|
L

W
|
W

C

T

C

C

N

G

A

A

G

G

H

H

G

S

R

R

K

K

A

E

I

I

S

Q

E

E

A

A

V

V

S

A

K

R

Q

Q

I

L

H

G

E

T

M

L

D

D

Y

Y

A

S

P

P

S

G

F
|
F

Q

Q

M

Y

G

G

H

H

P

P

L

L

A

S

F

F

E

Q

L

L

A

A

E

E

R

K

L

I

A

A

A

G

I

L

A

L

P

P

K

G

G

E

I

L

N

N

K

H

V

V

F

F

T

T

N

G

S
|
S

G
|
G

S
|
S

E

E

S

C

V

A

D

D

T

T

A

S

L

I

K

K

I

M

A

A

L

R

N

A

Y

Y

H

W

R

R

A

L

R

K

G

G

E

Q

A

P

T

Q

R

K

T

T

R

K

F

L

I

I

G

G

R

R

E

A

L

R

G

G

Y
|
Y

H
|
H

G
|
G

V

V

G

N

F

V

G

A

G

G

I

T

S

S

V

L

G

G

I

I

G

G

N

N

R

R

R

K

T

M

F

F

S

G

Q

Q

Q

-

L

-

L

L

Q

M

G

D

V

V

D

D

H

H

L

L

P

P

H

H

T

T

L

L

D

Q

I

P

Q

G

Q

M

N

-

A

A

F

F

C

T

K

R

G

G

L
x
M

P

A

E

Q

T

T

-

G

G

G

A

V

E

E

K

L

A

A

E

N

V

E

L

L

E

L

Q

K

L

L

V

I

T

E

L

L

H

H

G

D

A

A

E

S

N

N

I

I

A

A

V

V

I

I

V

V

E
|
E

P

P

M

M

S

S

G

G

S
|
S

A

A

G

G

V

V

I

L

L

V

P

P

Q
x
V

G
|
G

Y
|
Y

L
|
L

K
x
Q

R

R

L

L

R

R

E

E

I

I

T

C

R

D

K

Q

Y

H

G

N

I

I

L

L

I

I

F

F

D
|
D

E

E

V
|
V

I
|
I

T

T

G

A

F

F

G

G

R

R

V

L

G

G

D

T

A

Y

F

S

A

G

S

A

Q

E

R

Y

W
x
F

G
|
G

V
|
V

T

T

P

P

D

D

M

L

I

M

T

N

T

V

A

A

K
|
K

A

Q

L

V

N

T

N

N

G

G

T

A

I

V

P

P

M

M

G

G

A

A

V

V

L

I

I

A

D

S

A

E

I

I

Y

Y

D

D

A

T

S

F

M

M

D

N

A

Q

-

A

-

L

P

P

E

E

G

H

A

A

I

V

E

E

L

F

Y

S

H

H

G

G

Y
|
Y

T
|
T

Y

Y

S

S

G

A

H

H

P

P

V

V

A

A

A

C

A

A

A

G

I

L

A

A

T

A

L

L

D

D

I

I

Y

L

Q

A

E

R

E

D

Q

N

L

L

F

V

E

Q

R

Q

T

S

K

A

S

E

L

L

E

A

G

P

Y

H

F

F

E

E

K

K

A

G

V

L

H

H

S

G

L

L

K

Q

G

G

L

A

P

K

N

N

L

V

I

I

D

D

I

I

R

R

N

N

T

C

G

G

L

L

V

A

A

G

G

A

I

I

Q

Q

F

I

S

A

P

P

S

R

D

D

K

G

G

D

I

P

G

T

K

V

R

R

G

P

F

F

G

E

I

A

F

G

E

M

T

K

C

L

F

W

K

Q

N

Q

G

G

T

F

L

Y

V

V

R
|
R

A

F

T

G

A

G

D

D

T

T

I

L

A
x
Q

L

F

S

G

P

P

P

T

L

F

I

N

V

A

D

R

E

P

A

E

Q

E

I

L

D

D

Q

R

M

L

V

F

N

D

T

A

L

V

S

G

D

E

A

A

I

L

T

N

T

G

V

I

active site: F17 (= F22), Y146 (= Y151), E219 (= E226), D252 (= D259), I255 (= I262), K281 (= K288), Q414 (≠ A419)
binding pyridoxal-5'-phosphate: M189 (≠ L197), V232 (≠ Q239), G233 (= G240), Y234 (= Y241), L235 (= L242), Q236 (≠ K243), F270 (≠ W277), G271 (= G278), V272 (= V279)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: L53 (= L58), W54 (= W59), F82 (= F87), S112 (= S117), G113 (= G118), S114 (= S119), Y146 (= Y151), H147 (= H152), G148 (= G153), E219 (= E226), S224 (= S231), D252 (= D259), V254 (= V261), I255 (= I262), K281 (= K288), Y319 (= Y324), T320 (= T325), R407 (= R412), Q414 (≠ A419)

3a8uX Crystal structure of omega-amino acid:pyruvate aminotransferase
49% identity, 97% coverage: 15:445/446 of query aligns to 9:440/441 of 3a8uX

query
sites
3a8uX

L

L

E

D

H

A

Y

H

W

W

M

M

P

P

F

Y

T

T

A

A

N

N

R

R

Q

N

F

F

K

L

Q

R

S

D

P

P

R

R

L

L

L

I

A

V

Q

A

A

A

E

E

G

G

M

S

Y

W

Y

L

K

V

D

D

V

D

N

K

G

G

R

R

P

K

V

V

L

Y

D

D

G

S

T

L

A

S

G

G

L

L

W

W

C

T

C

C

N

G

A

A

G

G

H

H

G

T

R

R

K

K

A

E

I

I

S

Q

E

E

A

A

V

V

S

A

K

K

Q

Q

I

L

H

S

E

T

M

L

D

D

Y

Y

A

S

P

P

S

G

F

F

Q

Q

M

Y

G

G

H

H

P

P

L

L

A

S

F

F

E

Q

L

L

A

A

E

E

R

K

L

I

A

T

A

D

I

L

A

T

P

P

K

G

G

N

I

L

N

N

K

H

V

V

F

F

T

T

N

D

S
|
S

G
|
G

S
|
S

E

E

S

C

V

A

D

L

T

T

A

A

L

V

K

K

I

M

A

V

L

R

N

A

Y

Y

H

W

R

R

A

L

R

K

G

G

E

Q

A

A

T

T

R

K

T

T

R

K

F

M

I

I

G

G

R

R

E

A

L

R

G

G

Y
|
Y

H
|
H

G
|
G

V

V

G

N

F

I

G

A

G

G

I

T

S

S

V

L

G

G

I

V

G

N

G

G

N

N

R

R

R

K

T

L

F

F

S

G

Q

Q

Q

P

L

-

L

M

Q

Q

G

D

V

V

D

D

H

H

L

L

P

P

H

H

T

T

L

L

D

-

I

L

Q

A

Q

S

N

N

A

A

F

Y

C

S

K

R

G

G

L

M

P

P

-

K

E

E

T

G

G

G

A

I

E

A

K

L

A

A

E

D

V

E

L

L

E

L

Q

K

L

L

V

I

T

E

L

L

H

H

G

D

A

A

E

S

N

N

I

I

A

A

V

V

I

F

V

V

E
|
E

P

P

M

L

S

A

G

G

S

S

A

A

G

G

V

V

I

L

L

V

P

P

Q

E

G

G

Y

Y

L

L

K

K

R

R

L

N

R

R

E

E

I

I

T

C

R

N

K

Q

Y

H

G

N

I

I

L

L

I

V

F

F

D
|
D

E

E

V
|
V

I
|
I

T

T

G

G

F

F

G

G

R

R

V

T

G

G

D

S

A

M

F

F

A

G

S

A

Q

D

R

S

W

F

G

G

V

V

T

T

P

P

D

D

M

L

I

M

T

C

T

I

A

A

K
|
K

A

Q

L

V

N

T

N

N

G

G

T

A

I

I

P

P

M

M

G

G

A

A

V

V

L

I

I

A

D

S

D

T

A

E

I

I

Y

Y

D

Q

A

T

S

F

M

M

D

N

-

Q

-

P

A

T

P

P

E

E

G

Y

A

A

I

V

E

E

L

F

Y

P

H

H

G

G

Y

Y

T

T

Y

Y

S

S

G

A

H

H

P

P

V

V

A

A

A

C

A

A

A

G

I

L

A

A

T

A

L

L

D

C

I

L

Y

L

Q

Q

E

K

E

E

Q

N

L

L

F

V

E

Q

R

S

T

V

K

A

S

E

L

V

E

A

G

P

Y

H

F

F

E

E

K

K

A

A

V

L

H

H

S

G

L

I

K

K

G

G

L

A

P

K

N

N

L

V

I

I

D

D

I

I

R

R

N

N

T

F

G

G

L

L

V

A

A

G

G

A

I

I

Q

Q

F

I

S

A

P

P

S

R

D

D

K

G

G

D

I

A

G

I

K

V

R

R

G

P

F

F

G

E

I

A

F

G

E

M

T

A

C

L

F

W

K

K

N

A

G

G

T

F

L

Y

V

V

R

R

A

F

T

G

A

G

D

D

T

T

I

L

A
x
Q

L

F

S

G

P

P

P

T

L

F

I

N

V

S

D

K

E

P

A

Q

Q

D

I

L

D

D

Q

R

M

L

V

F

N

D

T

A

L

V

S

G

D

E

A

V

I

L

T

N

T

K

V

L

active site: Y145 (= Y151), D252 (= D259), K281 (= K288), Q414 (≠ A419)
binding pyridoxal-5'-phosphate: S111 (= S117), G112 (= G118), S113 (= S119), Y145 (= Y151), H146 (= H152), G147 (= G153), E219 (= E226), D252 (= D259), V254 (= V261), I255 (= I262), K281 (= K288)

6gwiB The crystal structure of halomonas elongata amino-transferase (see paper)
37% identity, 96% coverage: 17:442/446 of query aligns to 13:442/450 of 6gwiB

query
sites
6gwiB

H

H

Y

H

W

L

M

H

P

P

F
|
F

T

T

A

D

N

F

R

K

Q

A

F

L

-

G

K

E

Q

E

S

G

P

S

R

R

L

V

A

T

Q

H

A

A

E

E

G

G

M

V

Y

Y

Y

I

K

H

D

D

V

S

N

E

G

G

R

N

P

R

V

I

L

L

D

D

G

G

T

M

A

A

G

G

L

L

W

W

C

C

C

V

N

N

A

L

G

G

H

Y

G

G

R

R

K

R

A

E

I

L

S

V

E

E

A

A

V

A

S

T

K

A

Q

Q

I

L

H

E

E

Q

M

L

D

P

Y

Y

A

Y

P

N

S

T

F

F

-

F

Q

K

M

T

G

T

H

H

P

P

L

P

A

A

F

V

E

R

L

L

A

A

E

E

R

K

L

L

A

C

A

D

I

L

A

A

P

P

K

A

G

H

I

I

N

N

K

R

V

V

F

F

T

T

N

G

S
|
S

G
|
G

S
|
S

E

E

S

A

V
x
N

D

D

T

T

A

V

L

L

K

R

I

M

A

V

L

R

N

R

Y

Y

H

W

R

A

A

L

R

K

G

G

E

Q

A

P

T

D

R

K

T

Q

R

W

F

I

I

I

G

G

R

R

E

E

L

N

G

A

Y
|
Y

H
|
H

G
|
G

V

S

G

T

F

L

G

A

G

G

I

M

S

S

V

L

G

G

I

M

G

-

N

-

R

-

T

-

F

-

S

A

Q

P

Q

M

L

H

L

A

Q

Q

G

G

V

G

D

P

H

C

L

V

P

P

H

G

T

I

L

A

D

H

I

I

Q

R

Q

Q

N

P

-

Y

A

W

F

F

C

G

K

E

G

G

L

R

-

D

-

M

-

S

P

P

E

E

T

A

-

F

G

G

A

Q

E

T

K

C

A

A

E

E

V

A

L

L

E

E

Q

E

L

K

V

I

T

L

L

E

H

L

G

G

A

E

E

E

N

K

I

V

A

A

V

F

I

I

V

A

E
|
E

P

P

M

V

S

Q

G

G

S

A

A

G

G

G

V

A

I

I

L

M

P

P

Q

E

G

S

Y

Y

L

W

K

P

R

A

L

V

R

K

E

K

I

V

T

L

R

A

K

K

Y

Y

G

D

I

I

L

L

I

V

F

A

D
|
D

E

E

V
|
V

I
|
I

T

C

G

G

F

F

G

G

R

R

V

L

G

G

D

E

A

W

F

F

A

G

S

S

Q

Q

R

H

W

Y

G

G

V

L

T

E

P

P

D

D

M

L

I

M

T

P

T

I

A

A

K
|
K

A

G

L

L

N

S

G

G

T

Y

I

L

P

P

M

I

G

G

A

G

V

V

L

L

I

V

D

G

D

D

A

R

I

V

Y

A

D

E

A

T

S

L

M

I

D

E

A

-

P

-

E

E

G

G

A

G

I

-

E

E

L

F

Y

F

H

H

G

G

Y
x
F

T
|
T

Y

Y

S

S

G

G

H

H

P

P

V

T

A

C

A

A

A

V

A

A

I

L

A

K

T

N

L

L

D

E

I

L

Y

L

Q

E

E

A

E

E

Q

G

L

V

F

V

E

D

R

R

T

V

K

R

-

D

S

D

L

L

E

G

G

P

Y

Y

F

L

E

A

K

E

A

R

V

W

H

A

S

S

L

L

K

V

G

D

L

H

P

P

N

I

L

V

I

G

D

E

I

A

R

R

N

S

T

L

G

G

L

L

V

M

A

G

G

A

I

L

Q

E

F

L

S

V

P

-

S

A

D

D

K

K

G

T

I

T

G

G

K

Q

R

R

-

F

-

D

-

K

-

S

-

L

-

G

-

A

G

G

F

N

G

L

I

C

F

R

E

D

T

L

C

C

F

F

K

A

N

N

G

G

T

L

L

V

V

M

R

R

A

S

T

V

A

G

D

D

T

T

I

M

A

I

L

I

S

S

P

P

L

L

I

V

V

I

D

R

E

R

A

E

Q

E

I

I

D

D

Q

E

M

L

V

V

N

E

T

L

L

A

S

R

D

R

A

A

I

L

active site: F18 (= F22), Y149 (= Y151), D255 (= D259), K284 (= K288)
binding pyridoxal-5'-phosphate: S115 (= S117), G116 (= G118), S117 (= S119), N120 (≠ V122), Y149 (= Y151), H150 (= H152), G151 (= G153), E222 (= E226), D255 (= D259), V257 (= V261), I258 (= I262), K284 (= K288), F317 (≠ Y324), T318 (= T325)

6fyqA The crystal structure of a new transaminase from the marine bacterium virgibacillus (see paper)
34% identity, 96% coverage: 16:445/446 of query aligns to 9:438/443 of 6fyqA

query
sites
6fyqA

E

K

H

H

Y

F

W

I

M

H

P

P

F
|
F

T

S

A

S

-

I

-

Q

N

E

R

Q

Q

Q

F

H

K

K

Q

G

S

A

P

K

R

V

L

I

L

M

A

K

Q

E

A

G

E

D

G

G

M

I

Y

Y

Y

L

K

T

D

D

V

V

N

T

G

G

R

K

P

T

V

Y

L

I

D

D

G

G

T

V

A

S

G

S

L

L

W

W

C

N

C

V

N

N

A

V

G

G

H

H

G

G

R

R

K

V

A

E

I

L

S

A

E

E

A

A

V

A

S

A

K

Q

Q

Q

I

M

H

K

E

K

M

M

D

A

Y

F

A

S

P

S

S

A

F

F

Q

S

-

T

M

F

G

S

H

H

P

E

L

P

A

A

F

I

E

R

L

L

A

A

E

E

R

K

L

I

A

A

A

S

I

I

A

T

P

P

K

E

G

G

I

L

N

N

K

A

V

V

F

F

T

T

N

S

S
x
G

G
|
G

S
|
S

E

E

S

S

V
x
N

D

D

T

S

A

A

L

V

K

K

I

L

A

V

L

R

N

H

Y

Y

H

W

R

K

A

I

R

Q

G

G

E

K

A

P

T

N

R

K

T

R

R

K

F

I

I

I

G

S

R

L

E

K

L

R

G

S

Y
|
Y

H
|
H

G
|
G

V

V

G

A

F

A

G

A

G

S

I

T

S

S

V

V

G

T

G

G

I

I

-

P

-

E

-

F

G

W

G

G

N

M

R

A

R

G

T

H

F

M

S

M

Q

T

Q

D

L

F

L

L

Q

H

G

V

V

D

D

T

H

H

L

Y

P

N

H

N

T

T

L

T

D

E

I

Q

Q

A

Q

V

N

Q

A

S

F

L

C

C

K

Q

G

A

L

I

P

E

E

E

T

A

G

G

A

P

E

E

K

-

A

-

E

-

V

-

L

-

E

-

Q

-

L

-

V

-

T

-

L

-

H

-

G

-

A

-

E

-

N

T

I

I

A

A

V

F

I

F

V

A

E
|
E

P

P

M

V

S

Q

G

G

S

A

A

G

G

G

V

V

I

I

L

I

P

P

Q

E

G

D

Y

Y

L

F

K

L

R

R

L

I

R

R

E

E

I

V

T

C

R

N

K

A

Y

Y

G

G

I

I

L

L

L

F

I

V

F

A

D
|
D

E

E

V
|
V

I
|
I

T

T

G

G

F

F

G

G

R

R

V

T

G

G

D

K

A

M

F

F

A

G

S

I

Q

E

R

N

W

W

G

D

V

V

T

I

P

P

D

D

M

V

I

M

T

T

T

F

A

A

K
|
K

A

G

L

V

N

T

N

S

G

G

T

Y

I

F

P

P

M

L

G

G

A

G

V

V

L

V

I

V

D

S

D

D

A

P

I

I

Y

H

D

E

A

V

S

L

M

K

D

E

A

K

P

S

E

V

G

G

A

T

I

-

E

-

L

L

Y

F

H

H

G

G

Y

F

T

T

Y

Y

S

S

G

G

H

H

P

P

V

T

A

A

A

V

A

A

I

L

A

K

T

N

L

I

D

A

I

I

Y

I

Q

K

E

E

Q

R

L

L

F

V

E

E

R

N

T

S

K

K

S

R

L

M

E

G

G

-

Y

-

F

-

E

D

K

A

A

L

V

L

H

H

S

G

L

L

K

K

G

K

L

V

P

K

N

N

L

R

I

L

-

E

-

I

-

V

-

G

D

D

I

V

R

R

N

F

T

V

G

G

L

L

V

L

A

G

G

A

I

V

Q

E

F

L

-

M

-

Q

S

N

P

P

S

A

D

T

K

N

G

K

I

P

G

F

K

S

R

S

G

N

F

L

-

Q

-

V

-

A

-

P

G

K

I

V

F

I

E

E

T

A

C

L

F

H

K

E

N

L

G

G

T

V

L

I

V

C

R

R

A

S

T

V

-

T

-

Y

-

D

-

H

A

T

D

N

T

I

I

I

A

C

L

L

S

A

P

P

L

L

I

I

V

I

D

N

E

Q

A

K

Q

Q

I

V

D

D

Q

K

M

L

V

V

N

E

T

V

L

I

S

Y

D

E

A

A

I

I

T

L

T

K

V

V

active site: F15 (= F22), Y147 (= Y151), D243 (= D259), K272 (= K288)
binding pyridoxal-5'-phosphate: G113 (≠ S117), G114 (= G118), S115 (= S119), N118 (≠ V122), Y147 (= Y151), H148 (= H152), G149 (= G153), E210 (= E226), D243 (= D259), V245 (= V261), I246 (= I262), K272 (= K288)

5lhaA Amine transaminase crystal structure from an uncultivated pseudomonas species in the pmp-bound form
37% identity, 97% coverage: 16:446/446 of query aligns to 8:447/447 of 5lhaA

query
sites
5lhaA

E

K

H

K

Y

F

W

W

M

H

P

P

F

M

-

G

T

S

A

S

N

A

R

A

Q

P

F

H

K

R

Q

D

S

K

P

T

R

L

L

V

L

I

A

A

Q

R

A

G

E

D

G

G

M

N

Y

Y

Y

I

K

T

D

D

V

I

N

D

G

G

R

Q

P

R

V

M

L

L

D

D

G

G

T

V

A

G

G

G

L

L

W

W

C

N

C

V

N

N

A

I

G

G

H

H

G

N

R

R

K

A

A

S

I

V

S

K

E

A

A

A

V

I

S

A

K

A

Q

Q

I

L

H

D

E

E

M

L

D

A

Y

Y

A

Y

P

Q

S

T

F

F

Q

D

-

G

M

I

G

A

H

H

P

P

L

R

A

V

F

F

E

D

L

L

A

A

E

E

R

R

L

L

A

T

A

G

I

M

-

F

A

A

P

Q

K

E

G

R

I

M

N

A

K

R

V

V

F

L

F

F

T

S

N

S

S
x
G

G
|
G

S
|
S

E

D

S

A

V

V

D

E

T

T

A

A

L

L

K

K

I

M

A

A

L

R

N

Q

Y

Y

H

W

R

I

A

A

R

S

G

G

E

E

A

P

T

G

R

R

T

T

R

R

F

F

I

L

G

S

R

L

E

R

L

N

G

G

Y
|
Y

H
|
H

G
|
G

V

V

G

H

F

M

G

G

I

T

S

S

V

V

G

G

I

N

G

G

G

V

N

Y

R

H

R

Y

T

N

F

H

S

G

Q

Q

Q

-

L

L

Q

A

G

G

V

C

D

-

H

H

L

L

P

L

H

D

T

T

L

P

D

W

I

L

Q

Y

Q

R

N

N

A

P

F

W

-

D

C

C

K

R

G

D

L

P

P

Q

E

A

T

L

G

T

A

A

E

H

K

C

A

I

E

R

V

Q

L

L

E

E

Q

E

L

Q

V

I

T

A

L

L

H

L

G

G

A

A

E

Q

N

T

I

I

A

A

V

L

I

I

V

A

E
|
E

P

P

M

V

S

Q

G

G

S

A

A

G

G

G

V

V

I

I

L

V

P

P

Q

A

G

D

Y

Y

L

W

K

P

R

R

L

L

R

R

E

E

I

V

T

C

R

D

K

R

Y

H

G

G

I

I

L

L

I

I

F

A

D
|
D

E

E

V
|
V

I
x
V

T

T

G

G

F

F

G

G

R

R

V

S

G

G

D

C

A

M

F

L

A

G

S

S

Q

R

R

G

W

W

G

G

V

V

T

A

P

P

D

D

M

I

I

L

T

C

T

L

A

A

K
|
K

A

G

L

I

N

T

N

A

G

G

T

Y

I

I

P

P

M

L

G

G

A

A

V

T

L

L

I

F

D

N

D

Q

A

R

I

I

Y

A

D

D

A

A

S

I

M

E

D

N

A

G

P

Q

E

G

G

F

A

S

I

H

E

M

L

I

Y

M

H

H

G

G

Y
|
Y

T
|
T

Y

Y

S

S

G

G

H

H

P

P

V

T

A

A

A

C

A

A

I

L

A

A

T

V

L

L

D

D

I

I

Y

V

Q

E

E

A

E

E

Q

D

L

L

F

P

E

G

R

N

T

A

K

A

S

K

L

V

E

G

G

A

Y

Q

F

L

E

L

K

E

A

Q

V

L

H

Q

S

P

L

L

K

V

G

E

L

R

P

Y

N

A

L

V

I

V

-

G

D

E

I

V

R

R

N

G

T

K

G

G

L

L

V

M

A

I

G

A

I

L

Q

D

F

L

-

V

-

S

-

D

-

K

-

R

-

T

-

R

S

Q

P

P

S

L

D

D

K

P

G

A

I

A

G

G

K

Q

R

P

G

S

F

R

G

-

I

I

F

A

E

D

T

E

C

A

F

R

K

R

N

A

G

G

T

V

L

L

V

V

R

R

A

P

T

I

A

G

D

N

T

K

I

I

A

I

L

L

S

S

P

P

L

L

I

T

V

L

D

T

E

R

A

D

Q

E

I

A

D

G

Q

L

M

M

V

V

N

S

T

A

L

L

S

E

D

A

A

A

I

F

T

A

T

R

V

C

G

G

active site: Y146 (= Y151), D253 (= D259), K282 (= K288), T319 (= T325)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G112 (≠ S117), G113 (= G118), S114 (= S119), Y146 (= Y151), H147 (= H152), G148 (= G153), E220 (= E226), D253 (= D259), V255 (= V261), V256 (≠ I262), K282 (= K288), Y318 (= Y324), T319 (= T325)

5lh9D Amine transaminase crystal structure from an uncultivated pseudomonas species in the plp-bound (internal aldimine) form
37% identity, 97% coverage: 16:446/446 of query aligns to 10:449/449 of 5lh9D

query
sites
5lh9D

E

K

H

K

Y

F

W

W

M

H

P

P

F

M

-

G

T

S

A

S

N

A

R

A

Q

P

F

H

K

R

Q

D

S

K

P

T

R

L

L

V

L

I

A

A

Q

R

A

G

E

D

G

G

M

N

Y

Y

Y

I

K

T

D

D

V

I

N

D

G

G

R

Q

P

R

V

M

L

L

D

D

G

G

T

V

A

G

G

G

L

L

W

W

C

N

C

V

N

N

A

I

G

G

H

H

G

N

R

R

K

A

A

S

I

V

S

K

E

A

A

A

V

I

S

A

K

A

Q

Q

I

L

H

D

E

E

M

L

D

A

Y

Y

A

Y

P

Q

S

T

F

F

Q

D

-

G

M

I

G

A

H

H

P

P

L

R

A

V

F

F

E

D

L

L

A

A

E

E

R

R

L

L

A

T

A

G

I

M

-

F

A

A

P

Q

K

E

G

R

I

M

N

A

K

R

V

V

F

L

F

F

T

S

N

S

S
x
G

G
|
G

S
|
S

E

D

S

A

V

V

D

E

T

T

A

A

L

L

K

K

I

M

A

A

L

R

N

Q

Y

Y

H

W

R

I

A

A

R

S

G

G

E

E

A

P

T

G

R

R

T

T

R

R

F

F

I

L

G

S

R

L

E

R

L

N

G

G

Y
|
Y

H
|
H

G
|
G

V

V

G

H

F

M

G

G

I

T

S

S

V

V

G

G

I

N

G

G

G

V

N

Y

R

H

R

Y

T

N

F

H

S

G

Q

Q

Q

-

L

L

Q

A

G

G

V

C

D

-

H

H

L

L

P

L

H

D

T

T

L

P

D

W

I

L

Q

Y

Q

R

N

N

A

P

F

W

-

D

C

C

K

R

G

D

L

P

P

Q

E

A

T

L

G

T

A

A

E

H

K

C

A

I

E

R

V

Q

L

L

E

E

Q

E

L

Q

V

I

T

A

L

L

H

L

G

G

A

A

E

Q

N

T

I

I

A

A

V

L

I

I

V

A

E
|
E

P

P

M

V

S

Q

G

G

S

A

A

G

G

G

V

V

I

I

L

V

P

P

Q

A

G

D

Y

Y

L

W

K

P

R

R

L

L

R

R

E

E

I

V

T

C

R

D

K

R

Y

H

G

G

I

I

L

L

I

I

F

A

D
|
D

E

E

V
|
V

I
x
V

T

T

G

G

F

F

G

G

R

R

V

S

G

G

D

C

A

M

F

L

A

G

S

S

Q

R

R

G

W

W

G

G

V

V

T

A

P

P

D

D

M

I

I

L

T

C

T

L

A

A

K
|
K

A

G

L

I

N

T

N

A

G

G

T

Y

I

I

P

P

M

L

G

G

A

A

V

T

L

L

I

F

D

N

D

Q

A

R

I

I

Y

A

D

D

A

A

S

I

M

E

D

N

A

G

P

Q

E

G

G

F

A

S

I

H

E

M

L

I

Y

M

H

H

G

G

Y
|
Y

T
|
T

Y

Y

S

S

G

G

H

H

P

P

V

T

A

A

A

C

A

A

I

L

A

A

T

V

L

L

D

D

I

I

Y

V

Q

E

E

A

E

E

Q

D

L

L

F

P

E

G

R

N

T

A

K

A

S

K

L

V

E

G

G

A

Y

Q

F

L

E

L

K

E

A

Q

V

L

H

Q

S

P

L

L

K

V

G

E

L

R

P

Y

N

A

L

V

I

V

-

G

D

E

I

V

R

R

N

G

T

K

G

G

L

L

V

M

A

I

G

A

I

L

Q

D

F

L

-

V

-

S

-

D

-

K

-

R

-

T

-

R

S

Q

P

P

S

L

D

D

K

P

G

A

I

A

G

G

K

Q

R

P

G

S

F

R

G

-

I

I

F

A

E

D

T

E

C

A

F

R

K

R

N

A

G

G

T

V

L

L

V

V

R

R

A

P

T

I

A

G

D

N

T

K

I

I

A

I

L

L

S

S

P

P

L

L

I

T

V

L

D

T

E

R

A

D

Q

E

I

A

D

G

Q

L

M

M

V

V

N

S

T

A

L

L

S

E

D

A

A

A

I

F

T

A

T

R

V

C

G

G

active site: Y148 (= Y151), D255 (= D259), K284 (= K288), T321 (= T325)
binding pyridoxal-5'-phosphate: G114 (≠ S117), G115 (= G118), S116 (= S119), Y148 (= Y151), H149 (= H152), G150 (= G153), E222 (= E226), D255 (= D259), V257 (= V261), V258 (≠ I262), K284 (= K288), Y320 (= Y324), T321 (= T325)

6io1B Crystal structure of a novel thermostable (s)-enantioselective omega- transaminase from thermomicrobium roseum (see paper)
35% identity, 100% coverage: 2:445/446 of query aligns to 1:446/448 of 6io1B

query
sites
6io1B

E

E

D

T

F

W

N

N

K

A

A

A

D

E

Q

L

V

V

A

A

T

K

G

-

T

D

L

I

E

A

H

H

Y

H

W

L

M

H

P

P

F
x
L

T

T

A

N

N

L

R

Y

Q

Q

F

L

K

R

-

R

Q

E

S

G

P

P

R

L

L

V

L

L

A

V

Q

R

A

G

E

E

G

G

M

V

Y

W

Y

V

K

W

D

D

V

A

N

E

G

G

R

K

P

R

V

Y

L

L

D

D

G

G

T

F

A

A

G

G

L

L

W

W

C

N

C

V

N

N

A

I

G

G

H

H

G

G

R

R

K

R

A

E

I

L

S

A

E

E

A

A

V

A

S

R