SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 5208972 FitnessBrowser__PV4:5208972 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
27% identity, 94% coverage: 15:395/407 of query aligns to 15:410/425 of O59010

query
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O59010

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S65 (vs. gap) mutation to V: Strongly decreased chloride conductance.
R276 (= R263) mutation to S: Increased rate of aspartate transport; when associated with R-395.
RSS 276:278 (= RSS 263:265) binding
M311 (= M298) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
T314 (≠ A301) binding
V355 (= V342) binding
D394 (= D379) binding
M395 (≠ S380) mutation to R: Increased rate of aspartate transport; when associated with S-276.
R397 (≠ E382) mutation to A: Strongly decreased affinity for aspartate.
N401 (= N386) binding
D405 (= D390) mutation to N: Strongly decreased affinity for aspartate.

2nwwA Crystal structure of gltph in complex with tboa (see paper)
27% identity, 94% coverage: 15:395/407 of query aligns to 6:401/407 of 2nwwA

query
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2nwwA

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binding (3s)-3-(benzyloxy)-l-aspartic acid: S269 (= S265), T305 (≠ A301), A349 (≠ G345), G350 (≠ S346), D385 (= D379), R388 (≠ E382), T389 (= T383), N392 (= N386)

6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
27% identity, 94% coverage: 15:395/407 of query aligns to 7:402/408 of 6bauA

query
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6bauA

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binding cysteine: S270 (= S265), M303 (= M298), T306 (≠ A301), A345 (≠ S340), G346 (= G341), V347 (= V342), G351 (≠ S346), D386 (= D379), C389 (≠ E382), T390 (= T383), N393 (= N386)

6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
27% identity, 94% coverage: 15:395/407 of query aligns to 7:402/409 of 6bavA

query
sites
6bavA

S

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G

S

H

F

Y

S

G

P

Y

S

A

S

D

A

A

-

V

-

K

E

T

S

Y

V

V

A

K

F

P

L

F

G

G

D

D

L

L

F

F

V

V

G

R

A

L

L

L

K

K

A

M

I

L

A

V

P

M

I

P

L

I

V

V

F

F

-

A

-

S

I

L

L

V

V

V

A

G

A

A

S

A

I

S

A

I

N

S

Q

P

K

A

K

R

N

L

T

G

K

R

T

V

N

G

M

V

R

K

P

-

I

I

I

V

V

V

L

Y

Y

Y

L

L

F

L

G

T

T

S

F

A

A

F

A

A

A

V

I

T

T

L

A

G

V

I

T

I

M

M

S

A

F

R

L

L

F

F

P

N

-

P

-

G

T

A

S

G

L

I

V

H

L

L

V

A

S

V

G

G

A

G

Q

Q

G

Q

A

F

T

Q

P

P

P

K

E

Q

G

A

I

-

G

P

E

P

V

L

L

V

N

K

T

I

L

L

F

D

K

I

V

V

V

P

D

T

N

N

P

P

V

F

N

G

A

A

L

L

M

A

T

N

G

G

N

Q

Y

V

I

L

G

P

I

T

L

I

A

F

W

F

G

A

V

I

G

I

L

L

G

G

I

I

A

A

L

I

H

T

H

Y

-

L

-

M

A

N

S

S

E

E

N

N

T

E

K

K

T

V

-

R

-

K

-

S

-

A

-

E

V

T

F

L

A

L

D

D

V

A

S

I

H

N

G

G

V

L

S

A

Q

E

-

A

-

M

-

Y

-

K

M

I

V

V

R

N

F

G

V

V

I

M

R

Q

L

Y

A

A

P

P

I

I

G

G

I

V

F

F

G

A

L

L

V

I

A

A

N

Y

T

V

F

M

A

A

A

E

T

Q

G

G

F

V

E

K

A

V

I

V

A

G

G

E

Y

L

A

A

Q

K

L

V

L

T

A

A

V

A

L

V

L

Y

G

-

S

-

M

-

L

-

I

-

I

V

A

G

L

L

I

T

I

L

N

Q

P

I

L

L

I

L

V

V

W

Y

F

F

K

V

I

L

R

L

R

K

-

I

-

Y

-

G

-

I

N

D

P

P

Y

I

P

S

L

F

V

I

F

K

T

K

C

A

L

-

R

K

E

D

S

A

G

M

V

L

T

T

A

A

F

F

F

V

T

T

R
|
R

S

S

S
|
S

A

S

A

G

N

T

I

L

P

P

V

V

N

T

M

M

A

R

L

V

C

A

E

K

K

E

L

M

D

G

L

I

H

S

E

E

D

G

T

I

F

Y

S

S

V

F

S

T

I

L

P

P

L

L

G

G

A

A

T

T

I

I

N

N

M

M

A

D

G

G

A
x
T

A

A

I

L

T
x
Y

I

Q

T

G

V

V

L

C

T

T

L

F

A

F

A

I

V

A

H

N

T

A

E

L

G

G

I

S

Q

H

V

L

D

T

I

V

L

G

T

Q

A

Q

I

L

L

T

L

I

S

V

V

L

V

T

A

A

A

V

V

L

S

A

A

S

C

I

G

G

A

T

S

A

G

G

V

V

A

P

G

G

G

A

S

G

L

A

L

I

L

M

I

L

P

A

L

M

A

V

C

L

S

E

L

S

F

V

G

G

I

L

S

P

N

L

D

T

V

D

A

P

M

N

Q

V

V

A

A

A

V

Y

G

A

F

M

I

I

I

L

G

G

V

I

-
x
D

-

A

I

I

Q

L

D
|
D

S

M

A

G

E
x
C

T
|
T

A

M

L

V

N

N

S

V

S

T

T

G

D

D

V

L

L

T

F

G

T

T

A

A

binding benzylcysteine: R268 (= R263), S270 (= S265), T306 (≠ A301), Y309 (≠ T304), D382 (vs. gap), D386 (= D379), C389 (≠ E382), T390 (= T383)

6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
26% identity, 94% coverage: 15:395/407 of query aligns to 15:410/419 of 6x15A

query
sites
6x15A

S

K

L

I

V

L

L

I

Q

G

I

L

I

I

L

L

G

G

I

A

I

I

A

V

G

G

V

L

A

I

L

L

A

G

S

H

F

Y

S

G

P

Y

S

A

S

H

A

A

-

V

-

H

E

T

S

Y

V

V

A

K

F

P

L
x
F

G

G

D

D

L

L

F

F

V

V

G

R

A

L

L

L

K

C

A

M

I

L

A

V

P

M

I

P

L

I

V

V

F

F

-

A

-

S

I

L

L

V

V

V

A

G

A

A

S

A

I

S

A

I

N

S

Q
x
P

K

A

K

R

N

L

T

G

K

R

T

V

N

G

M

V

R

K

P

-

I

I

I

V

V

V

L

Y

Y
|
Y

L

L

F
x
L

G
x
T

T
x
S

F

A

A
x
F

A

A

A

V

I

T

T

L

A

G

V

I

T

I

M

M

S

A

F

R

L

L

F

F

P

N

-

P

-

G

T

A

S

G

L

I

V

H

L

L

V

A

S

V

G

G

A

G

Q

Q

G

Q

A

F

T

Q

P

P

P

H

E

Q

G

A

I

-

G

P

E

P

V
x
L

L

V

N

H

T
x
I

L

L

F

D

K

I

V

V

V

P

D

T

N

N

P

P

V

F

N

G

A

A

L

L

M

A

T

N

G

G

N

Q

Y

V

I

L

G

P

I

T

L

I

A

F

W

F

G

A

V
x
I

G

I

L

L

G

G

I

I

A

A

L

I

H

T

H
x
Y

-

L

-

M

A

N

S

S

E

E

N

N

T

E

K

K

T

V

-

R

-

K

-

S

-

A

-

E

V

T

F

L

A

L

D

D

V

A

S

I

H

N

G

G

V

L

S

A

Q

E

-

A

-

M

-

Y

-
x
K

M

I

V

V

R

N

F
x
G

V

V

I

M

R

Q

L

Y

A

A

P

P

I
|
I

G

G

I

V

F
|
F

G

A

L

L

V

I

A

A

N

Y

T

V

F

M

A

A

A

E

T

Q

G

G

F

V

E

H

A

V

I

V

A

G

G

E

Y

L

A

A

Q

K

L

V

L

T

A

A

V

A

L

V

L

Y

G

-

S

-

M

-

L

-

I

-

I

V

A

G

L

L

I

T

I

L

N

Q

P

I

L

L

I

L

V

V

W

Y

F

F

K

V

I
x
L

R

L

R

K

-

I

-

Y

-

G

-

I

N

D

P

P

Y

I

P

S

L

F

V
x
I

F

K

T

H

C

A

L

-

R

K

E

D

S

A

G
x
M

V

L

T

T

A

A

F

F

F

V

T

T

R

R

S
|
S

A

S

A

G

N

T

I

L

P

P

V

V

N

T

M

M

A

R

L

V

C

A

E

K

K

E

L

M

D

G

L

I

H

S

E

E

D

G

T

I

F

Y

S

S

V

F

S

T

I

L

P

P

L

L

G
|
G

A

A

T
|
T

I

I

N
|
N

M
|
M

A
x
D

G

G

A
x
T

A

A

I

L

T

Y

I

Q

T

G

V

V

L

A

T

T

L

F

A

F

A

I

V

A

H

N

T

A

E

L

G

G

I

S

Q

H

V

L

D
x
T

I
x
V

L
x
G

T

Q

A

Q

I

L

L
x
T

L

I

S

V

V
x
L

V

T

A

A

A

V

V

L

S

A

A
x
S

C
x
I

G

G

A
x
T

S

A

G
|
G

V

V

A

P

G

G

G
x
A

S
x
G

L

A

L

I

L

M

I

L

P

C

L

M

A

V

C

L

S

H

L

S

F

V

G

G

I

L

S

P

N

L

D

T

V

D

A

P

M

N

Q

V

V

A

A

A

V

Y

G

A

F

M

I

I

I

L

G

G

V

I

-

D

-

A

I

I

Q

L

D
|
D

S

M

A

G

E
x
R

T
|
T

A
x
M

L

V

N
|
N

S
x
V

S

T

T

G

D
|
D

V

L

L

T

F

G

T

T

A

A

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: F46 (≠ L44), F46 (≠ L44), P75 (≠ Q71), L91 (≠ F88), F95 (≠ A92), L130 (≠ V126), I133 (≠ T129), I159 (≠ V155), Y167 (≠ H163), K196 (vs. gap), G200 (≠ F185), I207 (= I192), F210 (= F195), L250 (≠ I240), I262 (≠ V248), M269 (≠ G256), T334 (≠ D321), V335 (≠ I322), G336 (≠ L323), T340 (≠ L327), L343 (≠ V330), M399 (≠ A384)
binding aspartic acid: S277 (= S264), S278 (= S265), T314 (≠ A301), G354 (= G341), A358 (≠ G345), G359 (≠ S346), D394 (= D379), R397 (≠ E382), T398 (= T383)
binding sodium ion: Y89 (= Y86), T92 (≠ G89), S93 (≠ T90), G306 (= G293), T308 (= T295), N310 (= N297), N310 (= N297), M311 (= M298), D312 (≠ A299), S349 (≠ A336), I350 (≠ C337), T352 (≠ A339), N401 (= N386), V402 (≠ S387), D405 (= D390)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 4, 7

6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
26% identity, 94% coverage: 15:395/407 of query aligns to 12:407/413 of 6x14A

query
sites
6x14A

S

K

L
x
I

V

L

L

I

Q

G

I

L

I

I

L

L

G

G

I

A

I

I

A

V

G

G

V

L

A

I

L

L

A

G

S

H

F

Y

S

G

P

Y

S

A

S

H

A

A

-

V

-

H

E

T

S

Y

V

V

A

K

F

P

L

F

G

G

D

D

L

L

F

F

V

V

G

R

A

L

L

L

K

C

A

M

I

L

A

V

P

M

I

P

L

I

V

V

F

F

-

A

-

S

I

L

L

V

V

V

A
x
G

A

A

S

A

I

S

A

I

N

S

Q

P

K

A

K

R

N

L

T

G

K

R

T

V

N

G

M

V

R

K

P

-

I

I

I
x
V

V

V

L

Y

Y

Y

L

L

F

L

G

T

T

S

F

A

A

F

A

A

A

V

I

T

T

L

A

G

V

I

T

I

M

M

S

A

F

R

L

L

F

F

P

N

-

P

-

G

T

A

S

G

L

I

V

H

L

L

V

A

S

V

G

G

A

G

Q

Q

G

Q

A

F

T

Q

P

P

P

H

E

Q

G

A

I

-

G

P

E

P

V

L

L

V

N

H

T

I

L

L

F

D

K

I

V

V

V

P

D

T

N

N

P

P

V

F

N

G

A

A

L

L

M

A

T

N

G

G

N

Q

Y

V

I

L

G

P

I

T

L

I

A

F

W

F

G

A

V

I

G
x
I

L

L

G

G

I

I

A

A

L

I

H

T

H
x
Y

-

L

-

M

A

N

S

S

E

E

N

N

T

E

K

K

T

V

-

R

-

K

-

S

-

A

-

E

V

T

F

L

A

L

D

D

V

A

S

I

H

N

G

G

V

L

S

A

Q

E

-

A

-

M

-

Y

-
x
K

M

I

V

V

R

N

F

G

V

V

I
x
M

R

Q

L

Y

A

A

P

P

I

I

G

G

I

V

F

F

G

A

L

L

V

I

A

A

N

Y

T

V

F

M

A

A

A

E

T

Q

G

G

F

V

E

H

A

V

I

V

A

G

G

E

Y

L

A

A

Q

K

L

V

L

T

A

A

V

A

L

V

L

Y

G

-

S

-

M

-

L

-

I

-

I

V

A

G

L

L

I

T

I

L

N

Q

P

I

L

L

I

L

V

V

W

Y

F

F

K

V

I

L

R

L

R

K

-

I

-

Y

-

G

-

I

N

D

P

P

Y

I

P

S

L

F

V

I

F

K

T

H

C

A

L

-

R

K

E

D

S

A

G

M

V

L

T

T

A

A

F

F

F

V

T

T

R

R

S

S

S
|
S

A

S

A

G

N

T

I

L

P

P

V

V

N

T

M

M

A

R

L

V

C

A

E

K

K

E

L

M

D

G

L

I

H

S

E

E

D

G

T

I

F

Y

S

S

V

F

S

T

I

L

P

P

L

L

G

G

A

A

T
|
T

I
|
I

N

N

M

M

A

D

G

G

A
x
T

A

A

I

L

T

Y

I

Q

T

G

V

V

L

A

T

T

L

F

A

F

A

I

V

A

H

N

T

A

E

L

G

G

I

S

Q

H

V

L

D

T

I

V

L

G

T

Q

A

Q

I

L

L

T

L

I

S

V

V

L

V

T

A

A

A

V

V

L

S

A

A

S

C
x
I

G

G

A

T

S

A

G

G

V

V

A

P

G

G

G

A

S
x
G

L

A

L

I

L

M

I

L

P

C

L

M

A

V

C

L

S

H

L

S

F

V

G

G

I

L

S

P

N

L

D

T

V

D

A

P

M

N

Q

V

V

A

A

A

V

Y

G

A

F

M

I

I

I
x
L

G

G

V

I

-

D

-

A

I

I

Q

L

D
|
D

S

M

A

G

E
x
R

T

T

A

M

L

V

N

N

S

V

S

T

T

G

D

D

V

L

L

T

F

G

T

T

A

A

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (≠ A65), V83 (≠ I83), I157 (≠ G156), Y164 (≠ H163), K193 (vs. gap), T305 (= T295), I306 (= I296), I347 (≠ C337)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (≠ L16), M199 (≠ I187), S275 (= S265), T311 (≠ A301), G356 (≠ S346), L384 (≠ I374), D391 (= D379), R394 (≠ E382)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 4

6bmiA Crystal structure of gltph r397c in complex with l-serine (see paper)
29% identity, 79% coverage: 15:334/407 of query aligns to 7:346/396 of 6bmiA

query
sites
6bmiA

S

K

L

I

V

L

L

I

Q

G

I

L

I

I

L

L

G

G

I

A

I

I

A

V

G

G

V

L

A

I

L

L

A

G

S

H

F

Y

S

G

P

Y

S

A

S

D

A

A

-

V

-

K

E

T

S

Y

V

V

A

K

F

P

L

F

G

G

D

D

L

L

F

F

V

V

G

R

A

L

L

L

K

K

A

M

I

L

A

V

P

M

I

P

L

I

V

V

F

F

-

A

-

S

I

L

L

V

V

V

A

G

A

A

S

A

I

S

A

I

N

S

Q

P

K

A

K

R

N

L

T

G

K

R

T

V

N

G

M

V

R

K

P

-

I

I

I

V

V

V

L

Y

Y

Y

L

L

F

L

G

T

T

S

F

A

A

F

A

A

A

V

I

T

T

L

A

G

V

I

T

I

M

M

S

A

F

R

L

L

F

F

P

N

-

P

-

G

T

A

S

G

L

I

V

H

L

L

V

A

S

V

G

G

A

G

Q

Q

G

Q

A

F

T

Q

P

P

P

K

E

Q

G

A

I

-

G

P

E

P

V

L

L

V

N

K

T

I

L

L

F

D

K

I

V

V

V

P

D

T

N

N

P

P

V

F

N

G

A

A

L

L

M

A

T

N

G

G

N

Q

Y

V

I

L

G

P

I

T

L

I

A

F

W

F

G

A

V

I

G

I

L

L

G

G

I

I

A

A

L

I

H

T

H

Y

A

L

-

M

-

N

S

S

E

E

N

N

T

E

K

K

T

V

-

R

-

K

-

S

-

A

-

E

V

T

F

L

A

L

D

D

V

A

S

I

H

N

G

G

V

L

S

A

Q

E

-

A

-

M

-

Y

-

K

M

I

V

V

R

N

F

G

V

V

I

M

R

Q

L

Y

A

A

P

P

I

I

G

G

I

V

F

F

G

A

L

L

V

I

A

A

N

Y

T

V

F

M

A

A

A

E

T

Q

G

G

F

V

E

K

A

V

I

V

A

G

G

E

Y

L

A

A

Q

K

L

V

L

T

A

A

V

A

L

V

L

Y

G

-

S

-

M

-

L

-

I

-

I

V

A

G

L

L

I

T

I

L

N

Q

P

I

L

L

I

L

V

V

W

Y

F

F

K

V

I

L

R

L

R

K

-

I

-

Y

-

G

-

I

N

D

P

P

Y

I

P

S

L

F

V

I

F

K

T

K

C

A

L

-

R

K

E

D

S

A

G

M

V

L

T

T

A

A

F

F

F

V

T

T

R
|
R

S

S

S
|
S

A

S

A

G

N

T

I

L

P

P

V

V

N

T

M

M

A

R

L

V

C

A

E

K

K

E

L

M

D

G

L

I

H

S

E

E

D

G

T

I

F

Y

S

S

V

F

S

T

I

L

P

P

L

L

G

G

A

A

T

T

I

I

N

N

M

M

A

D

G

G

A
x
T

A

A

I

L

T

Y

I

Q

T

G

V

V

L

C

T

T

-

F

-

I

-

A

-

N

-

A

L

L

A

G

A

S

V

H

H

L

T

T

E

V

G

G

I

Q

Q

Q

V

L

D

T

I

I

-

V

L

L

T

T

A

A

I

V

L

L

L

A

S

I

V

M

V

L

A

A

A

M

V

V

binding serine: R268 (= R263), S270 (= S265), T306 (≠ A301)

Sites not aligning to the query:

binding serine: 374, 378, 381

6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
27% identity, 96% coverage: 16:406/407 of query aligns to 11:410/416 of 6r7rA

query
sites
6r7rA

L

L

V

V

L

L

Q

G

I

A

I

V

L

F

G

G

I

L

I

I

A

A

G

G

V

-

A

-

L
x
H

A

F

S

G

F

Y

S

A

P

G

S

A

S

V

A

K

E

T

S

Y

V

I

A

K

F

P

L

F

G

G

D

D

L

L

F

F

V

V

G

R

A

L

L

L

K

K

A

M

-

L

I

V

A

M

P

P

I

I

L

V

V

L

F

A

I

S

L

L

V

V

-

V

-

G

A

A

S

S

I

I

A

S

N

P

Q

A

K

R

K

L

N

G

T

-

K

R

T

V

N

G

M

V

R

K

P

-

I

I

I

V

V

V

L

Y

Y

Y

L

L

F

A

G

T

T

S

F

A

A

M

A

A

A

V

I

F

T

F

A

G

V

L

T

I

M

V

S

G

F

R

L

L

F

F

P

N

T

V

S

G

L

A

-

N

V

V

L

N

V

L

S

G

G

S

A

G

Q

T

G

G

A

A

T

Q

P

P

E

S

G

-

I

-

G

-

E

-

V

L

L

V

N

Q

T

T

L

L

F

N

K

I

V

V

V

P

D

T

N

N

P

P

V

F

N

A

A

S

L

L

M

A

T

K

G

G

N

E

Y

V

I

L

G

P

I

V

L

I

A

F

W

F

G

A

V

I

G

I

L

L

G

G

I

I

A

A

L

I

H

T

H

Y

-

L

-

M

-

N

-

R

-

N

-

E

-

R

-

V

-

R

-

K

A

S

S

A

E

E

N

T

T

L

K

L

T

R

V

V

F

F

A

D

D

G

V

L

S

A

H

E

G

A

V

M

S

Y

Q

L

M

I

V

V

R

G

F

G

V

V

I

M

R

Q

L

Y

A

A

P

P

I

I

G

G

I

V

F

F

G

A

L

L

V

I

A

A

N

Y

T

V

F

M

A

A

A

E

T

Q

G

G

F

V

E

R

A

V

I
x
V

A

G

G

P

Y

L

A
|
A

Q

K

L

V

-

G

A

A

V

V

L

Y

L

T

G

G

S

L

M

F

L

L

I

Q

I

I

A

V

L

I

I

T

I

Y

N

F

P

I

L

L

I

L

V

K

W

V

F

F

K

G

I

I

R

-

N

D

P

P

Y

I

P

K

L

F

V

I

F

R

T

K

C

A

L

-

R

K

E

D

S

A

G

M

V

I

T

T

A

A

F

F

F

V

T

T

R
|
R

S

S

S
|
S

A

S

A

G

N

T

I

L

P

P

V

V

N

T

M

M

A

R

L

V

C

A

E

E

K

E

-

E

L

M

D

G

L

V

H

D

E

K

D

G

T

I

F

F

S

S

V

F

S

T

I

L

P

P

L

L

G

G

A

A

T

T

I

I

N

N

M
|
M

A

D

G

G

A
x
T

A

A

I

L

T

Y

I

Q

T

G

V

V

L

T

T

V

L

L

A

F

A

V

V

A

H

N

T

A

E

I

G

G

I

H

Q

P

V

L

D

T

I

L

L

G

T

Q

A

Q

I

L

L

V

S

V

V

L

V

T

A

A

A

V

V

L

S

A

A

S

C

I

G

G

A
x
T

S

A

G
|
G

V
|
V

A

P

G

G

G

A

S
x
G

L

A

L

I

L

M

I

L

P

A

L

M

A

V

C

L

S

Q

L

S

F

V

G

G

I

L

S

D

N

L

D

T

V

P

A

G

M

S

Q

P

V

V

-

A

V

L

A

A

V

Y

G

A

F

M

I

I

I

L

G

G

V

I

-

D

-

A

I

I

Q

L

D
|
D

S

M

A

G

E
x
R

T
|
T

A

M

L

V

N
|
N

S

V

S

T

T

G

D

D

V

L

L

A

F

G

T

T

A

V

A

I

A

V

C

A

E

K

A

T

A

E

E

K

R

E

Q

L

A

D

E

E

binding d-aspartic acid: R263 (= R263), S265 (= S265), M299 (= M298), T302 (≠ A301), T340 (≠ A339), G342 (= G341), V343 (= V342), G347 (≠ S346), D383 (= D379), R386 (≠ E382), T387 (= T383), N390 (= N386)
binding decyl-beta-d-maltopyranoside: H23 (≠ L30), V212 (≠ I210), A216 (= A214)

6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
28% identity, 96% coverage: 16:406/407 of query aligns to 16:419/425 of 6zgbA

query
sites
6zgbA

L

L

V

V

L

L

Q

G

I

A

I

V

L

F

G

G

I

L

I

I

A

A

G

G

V

-

A

-

L

H

A

F

S

G

F

Y

S

A

P

G

S

A

S

V

A

K

E

T

S

Y

V

I

A

K

F

P

L

F

G

G

D

D

L

L

F

F

V

V

G

R

A

L

L

L

K

K

A

M

-

L

I

V

A

M

P

P

I

I

L

V

V

L

F

A

I

S

L

L

V

V

-

V

-

G

A

A

S

S

I

I

A

S

N

P

Q

A

K

R

K

L

N

G

T

-

K

R

T

V

N

G

M

V

R

K

P

-

I

I

I

V

V

V

L

Y

Y

Y

L

L

F

A

G

T

T

S

F

A

A

M

A

A

A

V

I

F

T

F

A

G

V

L

T

I

M

V

S

G

F

R

L

L

F

F

P

N

T

V

-

G

-

A

S

N

L

V

V

N

L

L

V

G

S

S

G

G

-

T

-

G

-

K

A

A

Q

I

G

E

A

A

T

Q

P

P

E

S

G

-

I

-

G

-

E

-

V

L

L

V

N

Q

T

T

L

L

F

N

K

I

V

V

V

P

D

T

N

N

P

P

V

F

N

A

A

S

L

L

M

A

T

K

G

G

N

E

Y

V

I

L

G

P

I

V

L

I

A

F

W

F

G

A

V

I

G

I

L

L

G

G

I

I

A

A

L

I

H

T

H

Y

-

L

-

M

-

N

-

R

-

N

-

E

-

R

-

V

-

R

-

K

A

S

S

A

E

E

N

T

T

L

K

L

T

R

V

V

F

F

A

D

D

G

V

L

S

A

H

E

G

A

V

M

S

Y

Q

L

M

I

V

V

R

G

F

G

V

V

I

M

R

Q

L

Y

A

A

P

P

I

I

G

G

I

V

F

F

G

A

L

L

V

I

A

A

N

Y

T

V

F

M

A

A

A

E

T

Q

G

G

F

V

E

R

A

V

I

V

A

G

G

P

Y

L

A

A

Q

K

L

V

-

G

A

A

V

V

L

Y

L

T

G

G

S

L

M

F

L

L

I

Q

I

I

A

V

L

I

I

T

I

Y

N

F

P

I

L

L

I

L

V

K

W

V

F

F

K

G

I

I

R

-

N

D

P

P

Y

I

P

K

L

F

V

I

F

R

T

K

C

A

L

-

R

K

E

D

S

A

G

M

V

I

T

T

A

A

F

F

F

V

T

T

R
|
R

S
|
S

A

S

A

G

N

T

I

L

P

P

V

V

N

T

M

M

A

R

L

V

C

A

E

E

K

E

-

E

L

M

D

G

L

V

H

D

E

K

D

G

T

I

F

F

S

S

V

F

S

T

I

L

P

P

L

L

G

G

A

A

T

T

I

I

N

N

M
|
M

A

D

G

G

A

T

A

A

I

L

T

Y

I

Q

T

G

V

V

L

T

T

V

L

L

A

F

A

V

V

A

H

N

T

A

E

I

G

G

I

H

Q

P

V

L

D

T

I

L

L

G

T

Q

A

Q

I

L

L

V

S

V

V

L

V

T

A

A

A

V

V

L

S

A

A

S

C

I

G

G

A

T

S

A

G

G

V

V

A

P

G

G

G

A

S

G

L

A

L

I

L

M

I

L

P

A

L

M

A

V

C

L

S

Q

L

S

F

V

G

G

I

L

S

D

N

L

D

T

V

P

A

G

M

S

Q

P

V

V

-

A

V

L

A

A

V

Y

G

A

F

M

I

I

I

L

G

G

V

I

-

D

-

A

I

I

Q

L

D
|
D

S

M

A

G

E
x
R

T
|
T

A

M

L

V

N

N

S

V

S

T

T

G

D

D

V

L

L

A

F

G

T

T

A

V

A

I

A

V

C

A

E

K

A

T

A

E

E

K

R

E

Q

L

A

D

E

E

binding (2~{S},3~{S})-2-azanyl-3-[(2-nitrophenyl)methoxy]butanedioic acid: R272 (= R263), S273 (= S264), S274 (= S265), M308 (= M298), D392 (= D379), R395 (≠ E382), T396 (= T383)

5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
28% identity, 96% coverage: 16:406/407 of query aligns to 18:421/427 of 5e9sA

query
sites
5e9sA

L

L

V

V

L

L

Q

G

I

A

I

V

L

F

G

G

I

L

I

I

A

A

G

G

V

-

A

-

L

H

A

F

S

G

F

Y

S

A

P

G

S

A

S

V

A

K

E

T

S

Y

V

I

A

K

F

P

L

F

G

G

D

D

L

L

F

F

V

V

G

R

A

L

L

L

K

K

A

M

-

L

I

V

A

M

P

P

I

I

L

V

V

L

F

A

I

S

L

L

V

V

-

V

-

G

A

A

S

S

I

I

A

S

N

P

Q

A

K

R

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N

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-

K

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-

I

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Y

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x
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A

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-

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A

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L

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S

G

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-

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A

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A

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N

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A

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G

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L

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G

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I

A

A

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-

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-

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A

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A

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L

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A

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D

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G

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A

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A

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L

A

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L

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S

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G

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A

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T

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M
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M

A
x
D

G

G

A
x
T

A

A

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Y

I

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L

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A

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x
S

C
x
I

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G

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x
T

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G

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V

A

P

G

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x
A

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x
G

L

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A

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A

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N

L

D

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L

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x
R

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x
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binding aspartic acid: R274 (= R263), S275 (= S264), S276 (= S265), T313 (≠ A301), G353 (= G341), V354 (= V342), A357 (≠ G345), G358 (≠ S346), D394 (= D379), R397 (≠ E382), T398 (= T383)
binding decyl-beta-d-maltopyranoside: L194 (≠ Q181), G198 (≠ F185), Y202 (≠ L189)
binding sodium ion: Y87 (= Y86), T90 (≠ G89), S91 (≠ T90), S276 (= S265), G305 (= G293), A306 (= A294), T307 (= T295), N309 (= N297), N309 (= N297), M310 (= M298), D311 (≠ A299), S348 (≠ A336), I349 (≠ C337), G350 (= G338), T351 (≠ A339), N401 (= N386), V402 (≠ S387), D405 (= D390)

6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
28% identity, 96% coverage: 16:406/407 of query aligns to 15:418/424 of 6zl4A

query
sites
6zl4A

L

L

V

V

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L

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G

I

L

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I

A

A

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A

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L

H

A

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F

Y

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A

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D

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A

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L

L

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V

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Y

Y

Y

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A

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F

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L

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N

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A

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N

L

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N

L

L

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S

G

G

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G

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K

A

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A

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I

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G

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V

L

L

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N

Q

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T

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L

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N

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T

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x
A

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N

L

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x
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M

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N

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D

D

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L

L

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binding decyl-beta-d-maltopyranoside: L191 (≠ Q181), G195 (≠ F185), R282 (≠ A274)
binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (= R263), S272 (= S264), S273 (= S265), M307 (= M298), T310 (≠ A301), G353 (= G344), A354 (≠ G345), R394 (≠ E382), T395 (= T383)

Sites not aligning to the query:

binding decyl-beta-d-maltopyranoside: 3

6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
28% identity, 96% coverage: 16:406/407 of query aligns to 18:421/426 of 6xwnB

query
sites
6xwnB

L

L

V

V

L

L

Q

G

I

A

I

V

L

F

G

G

I

L

I

I

A

A

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A

-

L

H

A

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Y

S

A

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A

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L

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G

D

D

L

L

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F

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A

L

L

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K

A

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-

L

I

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I

I

L

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V

L

F

A

I

S

L

L

V

V

-

V

-

G

A

A

S

S

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I

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S

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A