SitesBLAST
Comparing 5209193 FitnessBrowser__PV4:5209193 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3bptA Crystal structure of human beta-hydroxyisobutyryl-coa hydrolase in complex with quercetin
36% identity, 91% coverage: 21:362/376 of query aligns to 15:351/362 of 3bptA
- active site: G67 (= G73), P84 (≠ Q94), R88 (≠ T98), G115 (= G125), G118 (= G128), E138 (= E148), D146 (= D156)
- binding (2r)-3-hydroxy-2-methylpropanoic acid: G66 (= G72), G67 (= G73), I69 (≠ V75), E90 (= E100), G114 (= G124), G115 (= G125), E138 (= E148), D146 (= D156), V147 (= V157)
- binding 3,5,7,3',4'-pentahydroxyflavone: F25 (≠ A31), L26 (= L32), A28 (= A34), G66 (= G72), G67 (= G73), I69 (≠ V75), P137 (= P147), I141 (= I151), L319 (= L334)
Q9LKJ1 3-hydroxyisobutyryl-CoA hydrolase 1; CoA-thioester hydrolase CHY1; EC 3.1.2.-; EC 3.1.2.4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
32% identity, 90% coverage: 21:357/376 of query aligns to 19:350/378 of Q9LKJ1
- G70 (= G73) mutation to S: Loss of activity.
- E142 (= E148) mutation to A: Loss of activity.
- D150 (= D156) mutation to G: Reduced activity.
4hdtA Crystal structure of a carnitinyl-coa dehydratase from mycobacterium thermoresistibile (see paper)
34% identity, 92% coverage: 21:367/376 of query aligns to 12:334/340 of 4hdtA
- active site: G64 (= G73), I69 (≠ L78), W84 (≠ F97), Y88 (= Y101), G112 (= G125), G115 (= G128), E135 (= E148), P142 (= P155), D143 (= D156), R283 (≠ Q311)
- binding zinc ion: H28 (≠ M37), E42 (≠ A51), E57 (= E66), E79 (= E88), H93 (≠ L106), H185 (≠ S199)
Sites not aligning to the query:
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
31% identity, 50% coverage: 10:196/376 of query aligns to 3:182/259 of 5zaiC
- active site: A65 (≠ G73), F70 (≠ L78), S82 (≠ F97), R86 (≠ Y101), G110 (= G125), E113 (≠ G128), P132 (= P147), E133 (= E148), I138 (≠ L153), P140 (= P155), G141 (≠ D156)
- binding coenzyme a: K24 (≠ A31), L25 (= L32), A63 (= A71), G64 (= G72), A65 (≠ G73), D66 (= D74), I67 (≠ V75), P132 (= P147), R166 (≠ N180)
Sites not aligning to the query:
6z1pBI mS93 (see paper)
26% identity, 46% coverage: 21:192/376 of query aligns to 32:201/1413 of 6z1pBI
- active site: T85 (≠ G73), S134 (≠ G125), E157 (= E148), D165 (= D156)
- binding : Y41 (≠ K30), K42 (≠ A31), Q43 (≠ L32), T45 (≠ A34), D47 (≠ N36), H49 (≠ E38), K83 (≠ A71), T85 (≠ G73), D86 (= D74), F87 (≠ V75), K88 (≠ R76), K92 (≠ Q80), L130 (≠ I121), K152 (≠ R143)
Sites not aligning to the query:
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
28% identity, 51% coverage: 9:200/376 of query aligns to 1:179/250 of 3q0gD
- active site: A64 (≠ G73), M69 (≠ L78), T75 (= T98), F79 (= F102), G103 (= G125), E106 (≠ G128), P125 (= P147), E126 (= E148), V131 (≠ L153), P133 (= P155), G134 (≠ D156)
Sites not aligning to the query:
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
29% identity, 51% coverage: 8:200/376 of query aligns to 1:184/255 of 3q0jC
- active site: A65 (≠ G73), M70 (≠ I89), T80 (= T98), F84 (= F102), G108 (= G125), E111 (≠ G128), P130 (= P147), E131 (= E148), V136 (≠ L153), P138 (= P155), G139 (≠ D156)
- binding acetoacetyl-coenzyme a: Q23 (≠ K30), A24 (= A31), L25 (= L32), A27 (= A34), A63 (= A71), G64 (= G72), A65 (≠ G73), D66 (= D74), I67 (≠ V75), K68 (≠ R76), M70 (≠ I89), F84 (= F102), G107 (= G124), G108 (= G125), E111 (≠ G128), P130 (= P147), E131 (= E148), P138 (= P155), G139 (≠ D156), M140 (≠ V157)
Sites not aligning to the query:
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
29% identity, 51% coverage: 8:200/376 of query aligns to 1:184/255 of 3q0gC
- active site: A65 (≠ G73), M70 (≠ I89), T80 (= T98), F84 (= F102), G108 (= G125), E111 (≠ G128), P130 (= P147), E131 (= E148), V136 (≠ L153), P138 (= P155), G139 (≠ D156)
- binding coenzyme a: L25 (= L32), A63 (= A71), I67 (≠ V75), K68 (≠ R76), Y104 (≠ I121), P130 (= P147), E131 (= E148), L134 (≠ I151)
Sites not aligning to the query:
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
29% identity, 51% coverage: 9:200/376 of query aligns to 1:183/256 of 3h81A
- active site: A64 (≠ G73), M69 (≠ I89), T79 (= T98), F83 (= F102), G107 (= G125), E110 (≠ G128), P129 (= P147), E130 (= E148), V135 (≠ L153), P137 (= P155), G138 (≠ D156)
Sites not aligning to the query:
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
31% identity, 45% coverage: 21:191/376 of query aligns to 47:208/290 of P14604
- E144 (≠ G128) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (= E148) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
31% identity, 45% coverage: 21:191/376 of query aligns to 15:176/258 of 1ey3A
- active site: A66 (≠ G73), M71 (≠ L78), S81 (≠ T98), L85 (= L103), G109 (= G125), E112 (≠ G128), P131 (= P147), E132 (= E148), T137 (≠ L153), P139 (= P155), G140 (≠ D156)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (= K30), L26 (= L32), A28 (= A34), A64 (= A71), G65 (= G72), A66 (≠ G73), D67 (= D74), I68 (≠ V75), L85 (= L103), W88 (≠ L106), G109 (= G125), P131 (= P147), L135 (≠ I151), G140 (≠ D156)
Sites not aligning to the query:
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
31% identity, 45% coverage: 21:191/376 of query aligns to 17:178/260 of 1dubA
- active site: A68 (≠ G73), M73 (≠ L78), S83 (≠ T98), L87 (= L103), G111 (= G125), E114 (≠ G128), P133 (= P147), E134 (= E148), T139 (≠ L153), P141 (= P155), G142 (≠ D156)
- binding acetoacetyl-coenzyme a: K26 (= K30), A27 (= A31), L28 (= L32), A30 (= A34), A66 (= A71), A68 (≠ G73), D69 (= D74), I70 (≠ V75), Y107 (≠ I121), G110 (= G124), G111 (= G125), E114 (≠ G128), P133 (= P147), E134 (= E148), L137 (≠ I151), G142 (≠ D156)
Sites not aligning to the query:
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
31% identity, 45% coverage: 21:191/376 of query aligns to 17:178/260 of 2hw5C
- active site: A68 (≠ G73), M73 (≠ L78), S83 (≠ T98), L87 (≠ F102), G111 (= G125), E114 (≠ G128), P133 (= P147), E134 (= E148), T139 (≠ L153), P141 (= P155), G142 (≠ D156)
- binding crotonyl coenzyme a: K26 (= K30), A27 (= A31), L28 (= L32), A30 (= A34), K62 (= K67), I70 (≠ V75), F109 (≠ M123)
Sites not aligning to the query:
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
31% identity, 45% coverage: 25:194/376 of query aligns to 19:182/261 of 5jbxB
- active site: A67 (≠ G73), R72 (≠ I89), L84 (= L103), R88 (≠ L107), G112 (= G125), E115 (≠ G128), T134 (≠ P147), E135 (= E148), I140 (≠ L153), P142 (= P155), G143 (≠ D156)
- binding coenzyme a: S24 (≠ K30), R25 (≠ A31), R26 (≠ L32), A28 (= A34), A65 (= A71), D68 (= D74), L69 (≠ V75), K70 (≠ R76), L110 (≠ M123), G111 (= G124), T134 (≠ P147), E135 (= E148), L138 (≠ I151), R168 (≠ N180)
Sites not aligning to the query:
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
30% identity, 45% coverage: 21:191/376 of query aligns to 17:176/258 of 1mj3A
- active site: A68 (≠ G73), M73 (≠ L78), S83 (≠ T98), L85 (≠ E100), G109 (= G125), E112 (≠ G128), P131 (= P147), E132 (= E148), T137 (≠ L153), P139 (= P155), G140 (≠ D156)
- binding hexanoyl-coenzyme a: K26 (= K30), A27 (= A31), L28 (= L32), A30 (= A34), A66 (= A71), G67 (= G72), A68 (≠ G73), D69 (= D74), I70 (≠ V75), G109 (= G125), P131 (= P147), E132 (= E148), L135 (≠ I151), G140 (≠ D156)
Sites not aligning to the query:
6iunB Crystal structure of enoyl-coa hydratase (ech) from ralstonia eutropha h16 in complex with NAD
33% identity, 39% coverage: 22:168/376 of query aligns to 9:144/692 of 6iunB
Sites not aligning to the query:
- active site: 248, 407, 428, 440, 478
- binding nicotinamide-adenine-dinucleotide: 300, 301, 302, 321, 322, 365, 377, 378, 380, 384, 388, 405, 407
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
30% identity, 45% coverage: 21:191/376 of query aligns to 16:172/254 of 2dubA
- active site: A67 (≠ G73), M72 (≠ L78), S82 (≠ T98), G105 (= G125), E108 (≠ G128), P127 (= P147), E128 (= E148), T133 (≠ L153), P135 (= P155), G136 (≠ D156)
- binding octanoyl-coenzyme a: K25 (= K30), A26 (= A31), L27 (= L32), A29 (= A34), A65 (= A71), A67 (≠ G73), D68 (= D74), I69 (≠ V75), K70 (≠ R76), G105 (= G125), E108 (≠ G128), P127 (= P147), E128 (= E148), G136 (≠ D156), A137 (≠ V157)
Sites not aligning to the query:
6eqoA Tri-functional propionyl-coa synthase of erythrobacter sp. Nap1 with bound NADP+ and phosphomethylphosphonic acid adenylate ester (see paper)
26% identity, 66% coverage: 18:267/376 of query aligns to 864:1109/1804 of 6eqoA
Sites not aligning to the query:
- binding phosphomethylphosphonic acid adenylate ester: 456, 458, 535, 536, 537, 538, 558, 559, 560, 561, 562, 688, 714
- binding nadp nicotinamide-adenine-dinucleotide phosphate: 1261, 1265, 1379, 1400, 1403, 1404, 1405, 1424, 1425, 1429, 1444, 1492, 1493, 1497, 1514, 1517, 1713, 1730, 1731, 1774
8oqlA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-1
32% identity, 34% coverage: 22:150/376 of query aligns to 22:151/728 of 8oqlA
Sites not aligning to the query:
- binding Hexafluorophosphate anion: 152, 180, 260, 362, 363, 364, 430, 476, 477, 478, 479, 506
8oqoA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-49
32% identity, 34% coverage: 22:150/376 of query aligns to 22:151/727 of 8oqoA
Sites not aligning to the query:
Query Sequence
>5209193 FitnessBrowser__PV4:5209193
MTEQTNCVVFQTLGTLSGKLVGVATLNMEKALNALNMEMVRALTTQLTAWADDDAIAMVV
LDGAGEKAFCAGGDVRALYQAARELPGEIPEVTQDFFTREYFLDHLLHTFDKPVLVWGDG
IVMGGGLGLMAGASHRVVTERSRIAMPEVTIGLYPDVGGSYFLNRMPGHMGRFLGMTAYN
MDGADAFYVGLGNHYLNSSDKQPLFDALSSIDWENDVCANHGKLNALLEAMSSRCAQPMG
ESILEQHQAQIDALMDGELNEIMTRVKAVEDAEPWLSRALSTMLAGSPLSLHLVYQQSLL
GTELSLSQVFQLELGLSCNCCSIGDFAEGVRALLIDKDRQPQWLYETVEAVPQEMVKSLM
TSPWDPQNDPLRTLGQ
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory