Comparing 5209394 Shew_1865 phosphoenolpyruvate-protein phosphotransferase (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 19 hits to proteins with known functional sites (download)
2hwgA Structure of phosphorylated enzyme i of the phosphoenolpyruvate:sugar phosphotransferase system (see paper)
37% identity, 99% coverage: 3:564/568 of query aligns to 1:565/572 of 2hwgA
P08839 Phosphoenolpyruvate-protein phosphotransferase; Phosphotransferase system, enzyme I; EC 2.7.3.9 from Escherichia coli (strain K12) (see 2 papers)
37% identity, 99% coverage: 3:564/568 of query aligns to 2:566/575 of P08839
P23533 Phosphoenolpyruvate-protein phosphotransferase; Phosphotransferase system, enzyme I; EC 2.7.3.9 from Staphylococcus carnosus (strain TM300) (see paper)
34% identity, 97% coverage: 3:552/568 of query aligns to 5:555/573 of P23533
2wqdA Crystal structure of enzyme i of the phosphoenolpyruvate:sugar phosphotransferase system in the dephosphorylated state (see paper)
33% identity, 97% coverage: 3:551/568 of query aligns to 4:554/570 of 2wqdA
2xz7A Crystal structure of the phosphoenolpyruvate-binding domain of enzyme i in complex with phosphoenolpyruvate from the thermoanaerobacter tengcongensis pep-sugar phosphotransferase system (pts) (see paper)
41% identity, 55% coverage: 251:563/568 of query aligns to 4:317/324 of 2xz7A
2xz9A Crystal structure from the phosphoenolpyruvate-binding domain of enzyme i in complex with pyruvate from the thermoanaerobacter tengcongensis pep-sugar phosphotransferase system (pts) (see paper)
41% identity, 54% coverage: 255:563/568 of query aligns to 1:310/317 of 2xz9A
5jvjB C4-type pyruvate phosphate dikinase: different conformational states of the nucleotide binding domain in the dimer (see paper)
25% identity, 65% coverage: 153:522/568 of query aligns to 350:779/797 of 5jvjB
Sites not aligning to the query:
5lu4A C4-type pyruvate phosphate dikinase: conformational intermediate of central domain in the swiveling mechanism (see paper)
25% identity, 65% coverage: 153:524/568 of query aligns to 423:836/850 of 5lu4A
Sites not aligning to the query:
Q39735 Pyruvate, phosphate dikinase, chloroplastic; Cold-sensitive pyruvate, orthophosphate dikinase; Pyruvate, orthophosphate dikinase; EC 2.7.9.1 from Flaveria bidentis (Coastal plain yellowtops) (Ethulia bidentis) (see 2 papers)
25% identity, 65% coverage: 153:524/568 of query aligns to 502:937/953 of Q39735
Sites not aligning to the query:
5jvlA C4-type pyruvate phospate dikinase: nucleotide binding domain with bound atp analogue (see paper)
25% identity, 65% coverage: 153:524/568 of query aligns to 423:858/874 of 5jvlA
Sites not aligning to the query:
Q02KR1 Phosphoenolpyruvate synthase; PEP synthase; Pyruvate, water dikinase; EC 2.7.9.2 from Pseudomonas aeruginosa (strain UCBPP-PA14) (see paper)
26% identity, 66% coverage: 157:530/568 of query aligns to 388:779/791 of Q02KR1
5jvlB C4-type pyruvate phospate dikinase: nucleotide binding domain with bound atp analogue (see paper)
26% identity, 60% coverage: 185:524/568 of query aligns to 122:504/520 of 5jvlB
Sites not aligning to the query:
1vbgA Pyruvate phosphate dikinase from maize (see paper)
25% identity, 65% coverage: 155:524/568 of query aligns to 425:858/874 of 1vbgA
Sites not aligning to the query:
1kc7A Pyruvate phosphate dikinase with bound mg-phosphonopyruvate (see paper)
25% identity, 60% coverage: 183:524/568 of query aligns to 449:854/872 of 1kc7A
Sites not aligning to the query:
P22983 Pyruvate, phosphate dikinase; Pyruvate, orthophosphate dikinase; EC 2.7.9.1 from Clostridium symbiosum (Bacteroides symbiosus) (see 4 papers)
25% identity, 60% coverage: 183:524/568 of query aligns to 450:855/874 of P22983
Sites not aligning to the query:
P11155 Pyruvate, phosphate dikinase 1, chloroplastic; Pyruvate, orthophosphate dikinase 1; EC 2.7.9.1 from Zea mays (Maize) (see 7 papers)
25% identity, 65% coverage: 155:524/568 of query aligns to 498:931/947 of P11155
Sites not aligning to the query:
O23404 Pyruvate, phosphate dikinase 1, chloroplastic; Pyruvate, orthophosphate dikinase 1; EC 2.7.9.1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
25% identity, 60% coverage: 183:524/568 of query aligns to 540:946/963 of O23404
1vbhA Pyruvate phosphate dikinase with bound mg-pep from maize (see paper)
26% identity, 60% coverage: 185:524/568 of query aligns to 441:846/862 of 1vbhA
Sites not aligning to the query:
P37349 PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM; Dihydroxyacetone kinase subunit M; EC 2.7.1.121 from Escherichia coli (strain K12) (see paper)
30% identity, 35% coverage: 2:198/568 of query aligns to 249:440/472 of P37349
Sites not aligning to the query:
>5209394 Shew_1865 phosphoenolpyruvate-protein phosphotransferase (RefSeq)
MTMRGIAVSAGIAFGQAKVLRTYESKLDYHLLPPSQLAREQQRLNRALKTLIKQSQACAA
KLDPESDNYQLIEADLLLLEDEELLAELSDTIGKRQFSAALAVEHSFAKQAQAMQEADSP
YLARRAEDVLSLGQRLIRTLLTGHCDNLSRLPENAIVLAKDITPAEFATLPLERVSALVL
QTGGVTSHTAILARSAGIPTLMSCPWQTLEVTDGMVLAVDAINGELYLEPDETQIDDLNA
QKQQADERKSALLALKGTVTQTKDGRSIPLLANVGCISEINHLADVGAEGVGLFRTEFLF
MNNHELPDENRQYQLYCDALQLLDGKPLTIRSMDLGADKEVPTLAMDSEENPALGLRGVR
YTLAHPKLFSAQLKAILRAANHGPIRLMFPMVNQVEELEAVLALLELCKQELVEAEKGFG
ELELGIVVETPAAVFNLASMLPLLDFVSIGTNDLTQYTMAADRANPLLIDQYPVLSPVII
RLIAQIIEQAKAAKVRVSMCGELASNPSATALLIGLGLEEFSVNLASLLEVKQALSRWSY
PDCVELAHKALQISRIDALNQLLTHCHS
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SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory