SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 5209403 FitnessBrowser__PV4:5209403 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

Q39232 Sucrose transport protein SUC1; AtSUC1; Sucrose permease 1; Sucrose-proton symporter 1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
26% identity, 40% coverage: 40:246/524 of query aligns to 42:271/513 of Q39232

query
sites
Q39232

G

G

I

V

Q

Q

F

F

G

G

F

W

A

A

L

L

Q

Q

N

L

A

S

N

L

V

L

S

T

R

P

I

Y

F

V

Q

Q

T

L

L

L

G

G

A

I

A

P

I
x
H

D

K

D

W

I

S

P

S

I

L

L

I

W

W

I

L

A

C

A

G

P

P

L

V

T

S

G

G

L

M

L

I

V

V

Q

Q

P

P

I

I

I

V

G

G

Y

F

M

H

S

S

D

D

N

R

T

C

W

R

G

S

R

K

L

F

G

G

R

R

R

R

R

R

P

P

Y

F

F

I

L

A

I

T

G

G

A

A

I

A

F

L

T

V

T

A

L

V

A

A

I

V

F

F

I

L

M

I

P

G

H

Y

S

A

-

A

-

D

-

F

-

G

-

Y

-

K

-

M

-

G

-

D

-

K

-

L

-

E

-

E

P

K

V

V

L

K

W

V

V

R

A

A

A

I

G

G

M

I

-

F

-

A

-

L

-

G

L

F

W

W

I

I

M

L

D

D

A

V

S

A

I

N

N

N

I

T

A

L

M

Q

E

G

P

P

F

C

R

R

A

A

F

F

V

L

G

A

D

D

-

L

-

A

N

A

L

G

P

D

T

A

K

K

Q

R

R

T

P

R

L

V

G

A

Y

N

A

A

M

F

Q

F

S

S

F

F

F

F

I

M

G

A

V

V

G

G

A

N

V

V

V

L

A

G

S

Y

A

A

-

A

-

G

-

S

-

Y

-

T

-

N

-

L

-

H

-

K

-

M

L

F

P

P

Y

F

L

T

L

M

T

T

N

K

Y

A

F

C

D

D

V

I

A

-

N

-

T

-

A

-

P

-

E

-

G

-

E

-

I

Y

A

C

D

A

S

N

V

L

R

K

Y

T

A

C

F

F

Y

F

F

L

G

S

G

I

A

T

V

L

L

L

L

L

L

I

A

V

V

T

S

V

W

T

T

S

V

L

F

W

-

Y

-

V

T

N

T

D

K

K

E

Q

Y

W

S

S

P

P

E

P

E

P

L

R

A

N

R

A

F

D

E

D

Q

D

E

E

N

K

G

T

E

S

Q

S

V

V

T

P

L

L

H

F

H

G

D

E

H65 (≠ I63) mutation to C: No sucrose transport activity, high instability.; mutation H->D,G,L,Y: Reduced sucrose transport activity.; mutation to K: Enhanced sucrose transport activity.; mutation to Q: Reduced sensitivity to DEPC.; mutation to R: Enhanced sucrose transport activity, reduced sensitivity to DEPC.; mutation to S: Reduced sucrose transport activity, reduced sensitivity to DEPC.

Query Sequence

>5209403 FitnessBrowser__PV4:5209403
MNNKEKHRMSQNTLPAQGTTTVKPALSFWQIFNMCFGFLGIQFGFALQNANVSRIFQTLG
AAIDDIPILWIAAPLTGLLVQPIIGYMSDNTWGRLGRRRPYFLIGAIFTTLAIFIMPHSP
VLWVAAGMLWIMDASINIAMEPFRAFVGDNLPTKQRPLGYAMQSFFIGVGAVVASALPYL
LTNYFDVANTAPEGEIADSVRYAFYFGGAVLLLAVSWTVFTTKEYSPEELARFEQENGEQ
VTLHHDRSWLAYQKGAMLWSLVGAILTAVIFLQSLDKQLYLLALGIFAFGPLQWFCANTL
KHIKGEERHQQGMVFSVVDALFHMPKAMRQLAVVQFFSWFALFSMWIYTTAAVTDHHYGT
QDVLSKAYNDGADWVGMLFASYNGFAAVAAIFIPLLAMAVGLKVTHLINLCCGGIGLISF
YFIQDPNLLWLPMIGVGIAWASILSIPYALLSNALPAAKMGVYMGIFNFFIVIPQLLAAS
VLGVLLNAFFDGKPIFALILGGSFMILAGISVLFVSSEASKAER

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory