SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 5209711 FitnessBrowser__PV4:5209711 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q3L887 Broad-specificity linear acyl-CoA dehydrogenase FadE5; Long-chain-acyl-CoA dehydrogenase; Medium-chain-acyl-CoA dehydrogenase; Short-chain-acyl-CoA dehydrogenase; EC 1.3.8.8; EC 1.3.8.7; EC 1.3.8.1 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
32% identity, 100% coverage: 1:595/596 of query aligns to 1:611/611 of Q3L887

query
sites
Q3L887

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MVLT 162:165 (≠ MNLT 162:165) binding
S171 (≠ T171) binding ; binding
T198 (≠ S198) binding
TK 224:225 (≠ VK 223:224) binding
R301 (= R295) binding
R326 (= R320) binding
K338 (≠ S332) binding
QTLGG 420:424 (≠ QVYGG 413:417) binding
E447 (= E440) binding ; mutation to A: Loss of activity.
T449 (= T442) binding
D456 (= D449) binding
RK 460:461 (= RK 453:454) binding

6lq8A Crystal structure of e447a acyl-coa dehydrogenase fade5 mutant from mycobacteria smegmatis in complex with c22coa (see paper)
32% identity, 100% coverage: 1:595/596 of query aligns to 1:607/607 of 6lq8A

query
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6lq8A

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binding coenzyme a: M162 (= M162), L164 (= L164), G170 (= G170), S171 (≠ T171), V173 (≠ L173), T224 (≠ V223), K225 (= K224), I290 (≠ L284), A291 (≠ R285), F294 (= F288), E298 (≠ N292), R301 (= R295), A447 (≠ E440), G448 (= G441), I452 (≠ V445), D456 (= D449), R460 (= R453), K461 (= K454), R464 (≠ A457)
binding docosanoic acid: V95 (≠ L93), G96 (≠ T94), L97 (≠ S95), Q111 (≠ G109), M127 (vs. gap), M130 (= M125), G133 (= G128), M134 (≠ L129), E136 (≠ H131), I137 (≠ G132), M162 (= M162), T198 (≠ S198), V296 (≠ M290), Q299 (≠ E293), A300 (= A294), M303 (≠ G297), Y446 (= Y439), A447 (≠ E440)
binding flavin-adenine dinucleotide: M162 (= M162), L164 (= L164), T165 (= T165), G170 (= G170), S171 (≠ T171), F196 (= F196), I197 (= I197), T198 (≠ S198), T266 (= T264), R326 (= R320), Q328 (= Q322), I345 (= I339), H348 (= H342), V351 (= V345), Q420 (= Q413), T421 (≠ V414), G423 (= G416), G424 (= G417), I442 (= I435), L445 (≠ I438), Y446 (= Y439), T449 (= T442), A451 (≠ G444), I452 (≠ V445)

6lq7A Crystal structure of e447a acyl-coa dehydrogenase fade5 mutant from mycobacteria smegmatis in complex with c17coa (see paper)
32% identity, 100% coverage: 1:595/596 of query aligns to 1:607/607 of 6lq7A

query
sites
6lq7A

M

M

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G

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W

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A

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A

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G

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A

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F

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Y

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N

G

K

K

L

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A

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A

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F

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F

M

A

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K

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N

W

M

A

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P

P

Q

L

T

L

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T

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S

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T

R

R

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Q

Q

I

M

I

E

E

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N

S

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L

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A

F

F

binding coenzyme a: M162 (= M162), L164 (= L164), S171 (≠ T171), V173 (≠ L173), T224 (≠ V223), K225 (= K224), I290 (≠ L284), A291 (≠ R285), F294 (= F288), E298 (≠ N292), R301 (= R295), A447 (≠ E440), G448 (= G441), I452 (≠ V445), D456 (= D449), R460 (= R453), K461 (= K454), R464 (≠ A457)
binding flavin-adenine dinucleotide: M162 (= M162), L164 (= L164), T165 (= T165), G170 (= G170), S171 (≠ T171), F196 (= F196), I197 (= I197), T198 (≠ S198), K258 (= K256), T266 (= T264), R326 (= R320), Q328 (= Q322), I345 (= I339), H348 (= H342), V351 (= V345), Q420 (= Q413), T421 (≠ V414), G423 (= G416), G424 (= G417), I442 (= I435), L445 (≠ I438), Y446 (= Y439), T449 (= T442), A451 (≠ G444)
binding heptadecanoic acid: M127 (vs. gap), M130 (= M125), G133 (= G128), M134 (≠ L129), A160 (≠ G160), T161 (= T161), M162 (= M162), T198 (≠ S198), V296 (≠ M290), Q299 (≠ E293), M303 (≠ G297), Y446 (= Y439), A447 (≠ E440)

6lq6A Crystal structure of e447a acyl-coa dehydrogenase fade5 mutant from mycobacteria smegmatis in complex with c20coa (see paper)
32% identity, 100% coverage: 1:595/596 of query aligns to 1:607/607 of 6lq6A

query
sites
6lq6A

M

M

P

S

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L

A

G

S

S

M

V

G

R

Y

F

L

L

Q

M

I

A

L

V

G

G

I

D

V

L

A

L

L

S

G

G

W

W

M

L

W

L

A

A

R

R

-

Q

-

A

M

V

A

A

V

I

K

E

S

K

Q

L

Q

D

A

A

M

G

T

A

A

T

G

G

E

A

E

D

E

K

A

S

F

F

Y

Y

A

E

N

G

K

K

L

I

V

A

T

A

A

A

K

S

F

F

Y

F

M

A

S

K

Y

N

W

M

A

L

P

P

Q

L

T

L

R

T

S

S

I

T

R

R

Y

Q

Q

I

M

I

E

E

R

N

S

L

C

D

E

N

L

D

L

V

T

M

Q

E

L

L

Q

D

D

E

S

A

D

A

F

F

binding coenzyme a: M162 (= M162), L164 (= L164), S171 (≠ T171), V173 (≠ L173), T224 (≠ V223), K225 (= K224), I290 (≠ L284), A291 (≠ R285), F294 (= F288), E298 (≠ N292), R301 (= R295), A447 (≠ E440), G448 (= G441), I452 (≠ V445), D456 (= D449), R460 (= R453), K461 (= K454), R464 (≠ A457)
binding icosanoic acid: G96 (≠ T94), L97 (≠ S95), M130 (= M125), G133 (= G128), M134 (≠ L129), E136 (≠ H131), I137 (≠ G132), A160 (≠ G160), T198 (≠ S198), Q299 (≠ E293), M303 (≠ G297), Y446 (= Y439), A447 (≠ E440)
binding flavin-adenine dinucleotide: M162 (= M162), V163 (≠ N163), L164 (= L164), T165 (= T165), G170 (= G170), S171 (≠ T171), F196 (= F196), I197 (= I197), T198 (≠ S198), K258 (= K256), T266 (= T264), R326 (= R320), Q328 (= Q322), I345 (= I339), H348 (= H342), V351 (= V345), Q420 (= Q413), T421 (≠ V414), G423 (= G416), G424 (= G417), I442 (= I435), L445 (≠ I438), Y446 (= Y439), T449 (= T442), A451 (≠ G444), I452 (≠ V445)

6lq1A Crystal structure of e447a acyl-coa dehydrogenase fade5 mutant from mycobacteria smegmatis in complex with c8coa (see paper)
32% identity, 100% coverage: 1:595/596 of query aligns to 1:607/607 of 6lq1A

query
sites
6lq1A

M

M

P

S

I

H

Y

Y

Q

K

A

S

P

N

L

V

R

R

D

D

Y

Q

Q

V

F

F

I

-

L

-

S

-

E

N

L

L

L

F

N

E

I

V

Y

F

G

G

R

V

D

D

D

K

L

V

Q

L

G

G

-

A

-

D

-

K

F

F

D

S

E

D

I

L

D

D

A

A

E

D

L

T

T

A

D

R

A

E

I

M

L

L

Q

T

G

E

V

I

A

A

D

R

F

L

T

A

T

E

E

G

I

P

M

I

L

A

P

E

L

S

N

F

S

V

Q

E

G

G

D

D

T

-

Q

R

G

N

C

P

Q

P

L

V

V

F

D

D

G

P

Q

E

V

T

R

H

T

T

-

V

-

T

-

L

P

P

E

E

G

G

F

F

K

K

D

K

A

S

Y

M

Q

R

Q

A

Y

L

V

F

D

D

N

G

G

G

W

W

A

D

T

K

L

V

T

G

S

L

D

A

P

E

E

H

Y

L

G

G

Q

I

G

P

L

M

P

P

E

R

C

A

I

L

G

Q

V

W

F

A

A

L

T

I

E

E

M

H

K

I

T

L

A

G

T

A

N

N

M

P

A

A

-

A

-

Y

-

M

F

Y

A

A

M
|
M

Y

G

P

P

G

G

L

M

T

S

H

E

G

I

A

F

Y

Y

A

N

A

-

I

-

H

-

A

-

H

N

G

G

S

T

D

D

A

E

L

Q

K

K

A

-

K

K

Y

W

L

A

E

T

K

I

L

A

V

A

S

E

G

R

E

G

W

W

T

G

G

A

T

T

M
|
M

N

V

L
|
L

T
|
T

E

E

A

P

H

D

A

A

G
|
G

T
x
S

D

D

L
x
V

A

G

L

A

L

G

R

R

T

T

K

K

A

A

K

V

P

Q

V

Q

G

P

E

D

D

G

T

T

Y

W

A

H

I

I

S

E

G

G

E

V

K

K

I

R

F
|
F

I
|
I

S
x
T

S

S

G

A

D

D

H

S

D

D

-

D

L

L

A

F

D

E

N

N

I

I

V

M

H

H

L

L

V

V

L

L

A

A

R

R

L

P

P

E

D

G

A

A

P

G

D

P

G

G

V
x
T

K
|
K

G

G

I

L

S

S

L

L

F

F

A

F

V

V

P

P

K

K

Y

F

-

H

L

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D

N

H

D

E

D

T

G

G

T

E

L

I

G

G

C

E

R

R

N

N

G

G

V

V

E

F

A

V

S

T

A

N

L

V

E

E

H

H

K

K

M

M

G

G

I

L

H

K

G

V

N

S

S

A

T
|
T

C

C

V

E

M

L

V

S

F

L

D

G

-

Q

-

H

-

G

-

I

G

P

A

A

I

V

G

G

E

W

L

L

V

V

G

G

E

E

P

V

H

H

Q

N

G

G

L
x
I

R
x
A

A

Q

M

M

F
|
F

T

D

M

V

M

I

N
x
E

E

Q

A

A

R
|
R

L

M

G
x
M

V

V

G

G

V

T

Q

K

G

A

L

I

G

A

V

T

S

L

E

S

I

T

A

G

Y

Y

Q

L

N

N

A

A

L

L

S

E

Y

Y

A

A

R

K

E

E

R
|
R

L

V

Q
|
Q

G

G

R

A

A

D

L

M

S

T

G

Q

V

M

K

T

E

D

S

K

E

T

S

A

P

P

A

R

D

V

P

T

I
|
I

L

T

V

H

H
|
H

G

P

D

D

V
|
V

R

R

M

S

L

L

M

M

A

T

Q

Q

K

K

A

A

F

Y

N

A

Q

E

G

G

A

L

R

R

A

A

L

I

M

Y

G

L

Q

Y

Q

T

A

A

L

T

W

F

L

Q

D

D

-

A

E

E

S

V

H

A

R

Q

H

A

Q

V

D

H

K

G

A

V

K

D

A

G

T

D

I

L

A

A

K

R

L

V

A

N

A

D

L

L

F

L

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L

P

P

V

I

V

V

K

K

G

G

F

F

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G

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T

G

A

F

Y

K

A

A

K

C

L

V

T

D

E

A

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Q

L

Q
|
Q

V
x
T

Y

L

G
|
G

H

S

G

G

Y

F

I

L

H

Q

E

D

W

Y

G

P

M

I

E

E

Q

Q

F

Y

V

I

R

R

D

D

S

S

R

K

I
|
I

A

D

M

S

I
x
L

Y
|
Y

E
x
A

G
|
G

T
|
T

N

T

G
x
A

V
x
I

Q

Q

A

A

L

Q

D
|
D

L

F

V

F

G

F

R
|
R

K
|
K

L

I

A
x
R

D

D

K

K

G

G

E

Q

A

A

L

L

G

A

L

Y

W

-

S

-

E

-

M

-

V

V

K

A

P

G

F

E

V

I

A

E

K

Q

Y

F

L

I

E

K

D

N

E

G

A

R

L

L

K

K

P

T

Y

E

L

R

M

E

P

L

L

L

M

A

E

T

A

A

T

L

T

A

D

D

L

V

E

Q

-

G

-

M

-

A

K

S

A

L

T

T

Q

G

Y

Y

I

L

V

M

A

A

N

A

G

Q

V

E

K

D

N

A

P

A

D

S

I

I

-

Y

-

K

I

V

G

G

A

L

A

G

S

S

M

V

G

R

Y

F

L

L

Q

M

I

A

L

V

G

G

I

D

V

L

A

L

L

S

G

G

W

W

M

L

W

L

A

A

R

R

-

Q

-

A

M

V

A

A

V

I

K

E

S

K

Q

L

Q

D

A

A

M

G

T

A

A

T

G

G

E

A

E

D

E

K

A

S

F

F

Y

Y

A

E

N

G

K

K

L

I

V

A

T

A

A

A

K

S

F

F

Y

F

M

A

S

K

Y

N

W

M

A

L

P

P

Q

L

T

L

R

T

S

S

I

T

R

R

Y

Q

Q

I

M

I

E

E

R

N

S

L

C

D

E

N

L

D

L

V

T

M

Q

E

L

L

Q

D

D

E

S

A

D

A

F

F

binding coenzyme a: M162 (= M162), L164 (= L164), S171 (≠ T171), V173 (≠ L173), T224 (≠ V223), K225 (= K224), I290 (≠ L284), A291 (≠ R285), F294 (= F288), E298 (≠ N292), R301 (= R295), A447 (≠ E440), G448 (= G441), I452 (≠ V445), D456 (= D449), R460 (= R453), K461 (= K454), R464 (≠ A457)
binding flavin-adenine dinucleotide: M162 (= M162), L164 (= L164), T165 (= T165), G170 (= G170), S171 (≠ T171), F196 (= F196), I197 (= I197), T198 (≠ S198), T266 (= T264), R326 (= R320), Q328 (= Q322), I345 (= I339), H348 (= H342), V351 (= V345), Q420 (= Q413), T421 (≠ V414), G423 (= G416), G424 (= G417), I442 (= I435), L445 (≠ I438), Y446 (= Y439), T449 (= T442), A451 (≠ G444), I452 (≠ V445)
binding octanoic acid (caprylic acid): M130 (= M125), M162 (= M162), M303 (≠ G297), Y446 (= Y439), A447 (≠ E440)

6lq0A Crystal structure of e447a acyl-coa dehydrogenase fade5 mutant from mycobacteria smegmatis in complex with c6coa (see paper)
32% identity, 100% coverage: 1:595/596 of query aligns to 1:607/607 of 6lq0A

query
sites
6lq0A

M

M

P

S

I

H

Y

Y

Q

K

A

S

P

N

L

V

R

R

D

D

Y

Q

Q

V

F

F

I

-

L

-

S

-

E

N

L

L

L

F

N

E

I

V

Y

F

G

G

R

V

D

D

D

K

L

V

Q

L

G

G

-

A

-

D

-

K

F

F

D

S

E

D

I

L

D

D

A

A

E

D

L

T

T

A

D

R

A

E

I

M

L

L

Q

T

G

E

V

I

A

A

D

R

F

L

T

A

T

E

E

G

I

P

M

I

L

A

P

E

L

S

N

F

S

V

Q

E

G

G

D

D

T

-

Q

R

G

N

C

P

Q

P

L

V

V

F

D

D

G

P

Q

E

V

T

R

H

T

T

-

V

-

T

-

L

P

P

E

E

G

G

F

F

K

K

D

K

A

S

Y

M

Q

R

Q

A

Y

L

V

F

D

D

N

G

G

G

W

W

A

D

T

K

L

V

T

G

S

L

D

A

P

E

E

H

Y

L

G

G

Q

I

G

P

L

M

P

P

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C

A

I

L

G

Q

V

W

F

A

A

L

T

I

E

E

M

H

K

I

T

L

A

G

T

A

N

N

M

P

A

A

-

A

-

Y

-

M

F

Y

A

A

M
|
M

Y

G

P

P

G

G

L

M

T

S

H

E

G

I

A

F

Y

Y

A

N

A

-

I

-

H

-

A

-

H

N

G

G

S

T

D

D

A

E

L

Q

K

K

A

-

K

K

Y

W

L

A

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T

K

I

L

A

V

A

S

E

G

R

E

G

W

W

T

G

G

A

T

T

M
|
M

N

V

L
|
L

T
|
T

E

E

A

P

H

D

A

A

G
|
G

T
x
S

D

D

L
x
V

A

G

L

A

L

G

R

R

T

T

K

K

A

A

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K

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F
|
F

I
|
I

S
x
T

S

S

G

A

D

D

H

S

D

D

-

D

L

L

A

F

D

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N

N

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H

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L

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L

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A

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T

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|
K

G

G

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L

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M

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|
T

C

C

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Q

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H

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I

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M

F
|
F

T

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M

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|
R

L

M

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M

V

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N

A

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L

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K

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|
Q

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|
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L

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H
|
H

G

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V
|
V

R

R

M

S

L

L

M

M

A

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Q

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K

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H

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G

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D

S

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|
I

A

D

M

S

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x
L

Y
|
Y

E
x
A

G
|
G

T
|
T

N

T

G
x
A

V
x
I

Q

Q

A

A

L
x
Q

D
|
D

L

F

V

F

G

F

R
|
R

K
|
K

L

I

A

R

D

D

K

K

G

G

E

Q

A

A

L

L

G

A

L

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binding hexanoic acid: M130 (= M125), M162 (= M162), T198 (≠ S198), M303 (≠ G297), Y446 (= Y439), A447 (≠ E440)
binding coenzyme a: M162 (= M162), L164 (= L164), S171 (≠ T171), V173 (≠ L173), T224 (≠ V223), K225 (= K224), I290 (≠ L284), A291 (≠ R285), F294 (= F288), E298 (≠ N292), R301 (= R295), A447 (≠ E440), G448 (= G441), I452 (≠ V445), D456 (= D449), R460 (= R453), K461 (= K454)
binding flavin-adenine dinucleotide: M162 (= M162), L164 (= L164), T165 (= T165), G170 (= G170), S171 (≠ T171), F196 (= F196), I197 (= I197), T198 (≠ S198), T266 (= T264), R326 (= R320), Q328 (= Q322), I345 (= I339), H348 (= H342), V351 (= V345), Q420 (= Q413), T421 (≠ V414), G423 (= G416), G424 (= G417), I442 (= I435), L445 (≠ I438), Y446 (= Y439), T449 (= T442), A451 (≠ G444), I452 (≠ V445), Q455 (≠ L448)

6lpyA Crystal structure of e447a acyl-coa dehydrogenase fade5 mutant from mycobacteria smegmatis in complex with c4coa (see paper)
32% identity, 100% coverage: 1:595/596 of query aligns to 1:607/607 of 6lpyA

query
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