SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 5209750 FitnessBrowser__PV4:5209750 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P60664 Imidazole glycerol phosphate synthase subunit HisF; IGP synthase cyclase subunit; IGP synthase subunit HisF; ImGP synthase subunit HisF; IGPS subunit HisF; EC 4.3.2.10 from Escherichia coli (strain K12) (see paper)
75% identity, 100% coverage: 1:257/257 of query aligns to 1:257/258 of P60664

query
sites
P60664

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R5 (= R5) mutation to A: Loss of activity.; mutation to H: Loss of IGP synthase activity. Weak IGP synthase activity and reduced HisH activity in vitro.
E46 (= E46) mutation to A: Loss of activity.; mutation to G: Loss of IGP synthase activity. Weak IGP synthase and HisH activities in vitro.
Q123 (= Q123) mutation to A: Decrease in activity.; mutation to R: Loss of IGP synthase activity. Weak HisH activity in vitro.
C124 (= C124) mutation to A: No change in activity.; mutation to R: Loss of IGP synthase activity. Weak HisH activity in vitro.

Q9X0C6 Imidazole glycerol phosphate synthase subunit HisF; IGP synthase cyclase subunit; IGP synthase subunit HisF; ImGP synthase subunit HisF; IGPS subunit HisF; EC 4.3.2.10 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
48% identity, 100% coverage: 1:257/257 of query aligns to 1:250/253 of Q9X0C6

query
sites
Q9X0C6

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C9 (= C9) mutation to A: No change in activity.
D11 (= D11) mutation to X: Loss of activity.
K19 (= K19) mutation to S: Decrease in activity.
D51 (= D51) mutation to N: No change in activity.
N103 (= N103) mutation to A: No change in activity.
D130 (= D130) mutation D->A,C,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y: Complete loss of activity.; mutation to E: Weak activity.
D176 (= D182) mutation to N: Decrease in activity.
D183 (= D189) mutation to N: No change in activity.

7ac8A Molecular basis for the unique allosteric activation mechanism of the heterodimeric imidazole glycerol phosphate synthase complex. (see paper)
48% identity, 100% coverage: 1:257/257 of query aligns to 1:250/252 of 7ac8A

query
sites
7ac8A

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active site: D11 (= D11), D130 (= D130)
binding [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate: L50 (≠ Y50), I52 (= I52), G82 (= G82), N103 (= N103), T104 (≠ S104), D130 (= D130), S144 (≠ T146), D176 (= D182), G177 (= G183), S201 (= S207), S225 (= S232)

1gpwC Structural evidence for ammonia tunneling across the (beta/alpha)8 barrel of the imidazole glycerol phosphate synthase bienzyme complex. (see paper)
48% identity, 100% coverage: 1:257/257 of query aligns to 1:250/253 of 1gpwC

query
sites
1gpwC

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active site: N11 (≠ D11), D130 (= D130)
binding phosphate ion: G82 (= G82), N103 (= N103), T104 (≠ S104), D176 (= D182), G177 (= G183), S225 (= S232)

7qc8A Imidazole glycerol phosphate synthase subunit HisF (see paper)
47% identity, 100% coverage: 2:257/257 of query aligns to 2:250/250 of 7qc8A

query
sites
7qc8A

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K

D

N

T

T

Q

G

W

I

Y

L

T

L

Q

R

D

D

W

W

V

V

E

V

E

E

V

V

Q

E

R

K

R

R

G

G

C

A

G

G

E

E

I

I

V

L

L

L

N

T

V

S

M

I

N

D

Q

R

D

D

G

G

V

T

R

K

Q

S

G

G

Y

Y

D

D

I

T

K

E

Q

M

L

I

A

R

M

F

V

V

R

R

A

P

I

L

C

T

D

T

V

L

P

P

L

I

I

I

A

A

S

S

G

G

A

A

G

G

T

K

M

M

E

E

H

H

F

F

L

L

E

E

V

A

F

F

T

L

Q

A

A

G

G

-

V

A

D

D

A

A

L

L

A

A

S

S

V

V

F

F

H

H

K

F

G

R

I

E

I

I

E

D

I

V

E

R

A

E

L

L

K

K

R

E

Y

Y

L

L

L

K

D

K

N

H

G

G

V

V

Q

N

M

V

R

R

L

L

binding zinc ion: E11 (≠ D11), H50 (≠ Y50), H52 (≠ I52)

5d2tA Directed evolutionary changes in kemp eliminase ke07 - crystal 3 wild type
45% identity, 99% coverage: 3:257/257 of query aligns to 1:248/251 of 5d2tA

query
sites
5d2tA

A

A

K

K

R

R

I

I

V

I

P

A

C

A

L

L

D

I

V

V

R

K

D

D

G

G

K

R

V

V

K

K

G

G

V

S

Q

N

F

F

R

E

N

N

H

L

E

R

I

D

V

S

G

G

D

D

I

P

V

V

P

E

L

L

A

G

A

K

R

F

Y

Y

A

S

A

E

E

I

S

G

A

I

D

D

E

E

L

L

V

S

F

F

Y
x
W

D

D

I

I

T

T

A

A

S

S

A

V

H

E

D

K

R

R

V

K

V

T

D

M

K

L

S

E

W

L

V

V

S

E

R

K

V

V

A

A

E

E

Q

Q

I

I

D

D

I

I

P

P

F

F

C

T

V

V

A

G

G

G

I

I

K

H

S

D

I

F

E

E

Q

T

A

A

R

S

E

E

K

L

L

I

A

L

F

R

G

G

A

A

D

D

K

K

I

V

S
x
E

I

I

N

N

S

T

P

A

A

A

L

V

S

E

D

N

P

P

G

S

L

L

I

I

D

T

R

Q

L

I

Q

A

D

Q

E

T

F

F

G

G

R

S

Q

Q

C

A

I

V

V

V

I

V

G

-

I

-

D

-

S

-

Y
|
Y

Y

I

D

A

A

A

D

K

T

R

D

V

S

D

Y

G

K

E

V

F

K

M

Q

V

F

F

T

T

G

-

D

-

E

Y

A

S

A

G

T

K

K

K

D

N

T

T

Q

G

W

I

Y

L

T

L

Q

R

D

D

W

W

V

V

E

V

E

E

V

V

Q

E

R

K

R

R

G

G

C

A

G

G

E

E

I

I

V

V

L

L

N

G

V

S

M

I

N

D

Q

R

D

L

G

G

V

T

R

K

Q

S

G

G

Y

Y

D

D

I

T

K

E

Q

M

L

I

A

R

M

F

V

V

R

R

A

P

I

L

C

T

D

T

V

L

P

P

L

I

I

I

A

A

S

H

G

G

A

A

G

G

T

K

M

M

E

E

H

H

F

F

L

L

E

E

V

A

F

F

T

L

Q

-

A

A

G

G

V

A

D

D

A

A

L
x
K

A

A

A

N

S

S

V

V

F

L

H

H

K

F

G

R

I

E

I

I

E

D

I

V

E

R

A

E

L

L

K

K

R

E

Y

Y

L

L

L

K

D

K

N

H

G

G

V

V

Q

N

M

V

R

R

L

L

binding 5-nitro-2-oxidanyl-benzenecarbonitrile: W48 (≠ Y50), E99 (≠ S101), Y126 (= Y132), K220 (≠ L229)

3zr4E Structural evidence for ammonia tunneling across the (beta-alpha)8 barrel of the imidazole glycerol phosphate synthase bienzyme complex (see paper)
47% identity, 100% coverage: 1:257/257 of query aligns to 1:241/244 of 3zr4E

query
sites
3zr4E

M

M

L

L

A

A

K

K

R

R

I

I

V

I

P

A

C

C

L

L

D
|
D

V

V

R

K

D

D

G

G

K

R

V

V

K

K

G

S

V

-

Q

-

F

-

R

-

N

-

H

-

E

-

I

-

V

-

G

G

D

D

I

P

V

V

P

E

L

L

A

G

A

K

R

F

Y

Y

A

S

A

E

E

I

S

G

A

I

D

D

E

E

L

L

V

V

F

F

Y

L

D

D

I

I

T

T

A

A

S

S

A

V

H

E

D

K

R

R

V

K

V

T

D

M

K

L

S

E

W

L

V

V

S

E

R

K

V

V

A

A

E

E

Q

Q

I

I

D

D

I

I

P

P

F

F

C

T

V

V

A

G

G

G

I

I

K

H

S

D

I

F

E

E

Q

T

A

A

R

S

E

E

K

L

L

I

A

L

F

R

G

G

A

A

D

D

K

K

I

V

S

S

I

I

N

N

S

T

P

A

A

A

L

V

S

E

D

N

P

P

G

S

L

L

I

I

D

T

R

Q

L

I

Q

A

D

Q

E

T

F

F

G

G

R

S

Q

Q

C

A

I

V

V

V

I

V

G

A

I

I

D
|
D

S

A

-

K

Y

R

Y

V

D

D

A

G

D

E

T

-

D

-

S

-

Y

F

K

M

V

V

K

F

Q

T

F

Y

T

S

G

G

D

K

E

-

A

-

T

-

K

K

D

N

T

T

Q

G

W

I

Y

L

T

L

Q

R

D

D

W

W

V

V

E

V

E

E

V

V

Q

E

R

K

R

R

G

G

C

A

G

G

E

E

I

I

V

L

L

L

N

T

V

S

M

I

N

D

Q

R

D

D

G

G

V

T

R

K

Q

S

G

G

Y

Y

D

D

I

T

K

E

Q

M

L

I

A
x
R

M

F

V

V

R

R

A

P

I

L

C

T

D

T

V

L

P

P

L

I

I

I

A

A

S

S

G

G

A

A

G

G

T

K

M

M

E

E

H

H

F

F

L

L

E

E

V

A

F

F

T
x
L

Q

A

A

G

G

-

V

A

D

D

A

A

L

L

A

A

S

S

V

V

F

F

H

H

K

F

G

R

I

E

I

I

E

D

I

V

E

R

A

E

L

L

K

K

R

E

Y

Y

L

L

L

K

D

K

N

H

G

G

V

V

Q

N

M

V

R

R

L

L

active site: D11 (= D11), D121 (= D130)
binding glycerol: R179 (≠ A194), L206 (≠ T221)

6dnjA Directed evolutionary changes in kemp eliminase ke07 - crystal 28 round 5 (see paper)
45% identity, 100% coverage: 2:257/257 of query aligns to 1:249/250 of 6dnjA

query
sites
6dnjA

L

L

A

A

K

K

R

R

I

I

V

D

P

A

C

A

L

L

D

I

V

M

R

K

D

D

G

G

K

R

V

V

K

K

G

G

V

S

Q

N

F

F

R

E

N

N

H

L

E

R

I

D

V

S

G

G

D

D

I

P

V

V

P

E

L

L

A

G

A

K

R

F

Y

Y

A

S

A

E

E

I

S

G

A

I

D

D

E

E

L

L

V

S

F

F

Y
x
W

D

D

I

I

T

T

A

A

S

S

A

V

H

E

D

K

R

R

V

K

V

T

D

M

K

L

S

E

W

L

V

V

S

E

R

K

V

V

A

A

E

E

Q

Q

I

I

D

D

I

I

P

P

F

F

C

T

V

V

A
x
G

G
|
G

G

G

I

I

K

H

S

D

I

F

E

E

Q

T

A

A

R

S

E

E

K

L

L

I

A

L

F

R

G

G

A

A

D

D

K

K

I

V

S

E

I

I

N
|
N

S

T

P

A

A

A

L

V

S

E

D

N

P

P

G

S

L

L

I

I

D

T

R

Q

L

I

Q

A

D

Q

E

T

F

F

G

G

R

S

Q

Q

C

A

I

V

V

V

I

V

G

-

I

-

D

-

S

-

Y
|
Y

Y

I

D

A

A

A

D

K

T

R

D

V

S

D

Y

G

K

E

V

F

K

M

Q

V

F

F

T

T

G

-

D

-

E

Y

A

S

A

G

T

K

K

K

D

N

T

T

Q

G

W

I

Y

L

T

L

Q

R

D

D

W

W

V

V

E

V

E

E

V

V

Q

E

R

K

R

R

G

G

C

A

G

G

E

E

I

I

V

V

L

L

N

G

V

S

M

I

N

D

Q

R

D

L

G

G

V

T

R

K

Q

S

G

G

Y

Y

D

D

I

T

K

E

Q

M

L

I

A

R

M

F

V

V

R

R

A

P

I

L

C

T

D

T

V

L

P

P

L

I

I

I

A

A

S

H

G

R

G

G

A

A

G

G

T

K

M

M

E

E

H

H

F

F

L

L

E

E

V

A

F

F

T

L

Q

-

A

A

G

G

V

A

D

D

A

A

L

K

A

A

A

D

S

S

V

V

F

F

H

H

K

F

G

R

I

E

I

I

E

D

I

V

E

R

A

E

L

L

K

K

R

E

Y

Y

L

L

L

K

D

K

N

H

G

G

V

V

Q

N

M

V

R

R

L

L

binding 5-nitro-1,2-benzoxazole: W49 (≠ Y50), G79 (≠ A80), G80 (= G81), N102 (= N103), Y127 (= Y132)

4ewnD Structure of hisf-d130v+d176v with bound rcdrp (see paper)
46% identity, 100% coverage: 2:257/257 of query aligns to 1:243/243 of 4ewnD

query
sites
4ewnD

L

L

A

A

K

K

R

R

I

I

V

I

P

A

C

C

L

L

D

D

V

V

R

K

D

D

G

G

K

R

V

V

K

K

G

G

V

T

Q

N

F

F

R

E

N

N

H

L

E

R

I

D

V

S

G

G

D

D

I

P

V

V

P

E

L

L

A

G

A

K

R

F

Y

Y

A

S

A

E

E

I

S

G

A

I

D

D

E

E

L

L

V

V

F

F

Y

L

D

D

I

I

T

T

A

K

S

T

A

M

H

L

D

E

R

-

V

-

D

-

K

-

S

-

W

L

V

V

S

E

R

K

V

V

A

A

E

E

Q

Q

I

I

D

D

I

I

P

P

F

F

C

T

V

V

A

G

G

G

I

I

K

H

S

D

I

F

E

E

Q

T

A

A

R

S

E

E

K

L

L

I

A

L

F

R

G

G

A

A

D

D

K

K

I

V

S

S

I

I

N

N

S

T

P

A

A

A

L

V

S

E

D

N

P

P

G

S

L

L

I

I

D

T

R

Q

L

I

Q

A

D

Q

E

T

F

F

G

G

R

S

Q

Q

C

A

I

V

V

V

I

V

G

A

I

I

-
x
V

D

A

S

K

Y

R

Y

V

D

D

A

G

D

E

T

-

D

-

S

-

Y

F

K

M

V

V

K

F

Q

T

F

Y

T

S

G

G

D

K

E

-

A

-

T

-

K

K

D

N

T

T

Q

G

W

I

Y

L

T

L

Q

R

D

D

W

W

V

V

E

V

E

E

V

V

Q

E

R

K

R

R

G

G

C

A

G

G

E

E

I

I

V

L

L

L

N

T

V

S

M

I

N

D

Q

R

D

V

G
|
G

V

T

R

K

Q

S

G

G

Y

Y

D

D

I

T

K

E

Q

M

L

I

A

R

M

F

V

V

R

R

A

P

I

L

C

T

D

T

V

L

P

P

L

I

I

I

A

A

S
|
S

G
|
G

A

A

G

G

T

K

M

M

E

E

H

H

F

F

L

L

E

E

V

A

F

F

T

L

Q

A

A

G

G

-

V

A

D

D

A

A

L
|
L

A
|
A

S
|
S

V

V

F

F

H

H

K

F

G

R

I

E

I

I

E

D

I

V

E

R

A

E

L

L

K

K

R

E

Y

Y

L

L

L

K

D

K

N

H

G

G

V

V

Q

N

M

V

R

R

L

L

binding 1-(O-carboxy-phenylamino)-1-deoxy-D-ribulose-5-phosphate: V123 (vs. gap), G170 (= G183), S194 (= S207), G195 (= G208), G196 (= G209), L215 (= L229), A216 (= A230), A217 (= A231), S218 (= S232)

3iivB Evolutionary optimization of computationally designed enzymes: kemp eliminases of the ke07 series (see paper)
45% identity, 100% coverage: 2:257/257 of query aligns to 2:249/262 of 3iivB

query
sites
3iivB

L

L

A

A

K

K

R

R

I

I

V

D

P

A

C

A

L

L

D

I

V

L

R

K

D

D

G

G

K

R

V

V

K

K

G

G

V

S

Q

N

F

F

R

E

N

N

H

L

E

R

I

D

V

S

G

G

D

D

I

P

V

V

P

E

L

L

A

G

A

K

R

F

Y

Y

A

S

A

E

E

I

S

G

A

I

D

D

E

E

L

L

V

S

F

F

Y

W

D

D

I

I

T

T

A

A

S

S

A

V

H

E

D

K

R

K

V

T

V

M

D

-

K

L

S

E

W

L

V

V

S

E

R

K

V

V

A

A

E

E

Q

Q

I

I

D

D

I

I

P

P

F

I

C

T

V

V

A

G

G

G

I

I

K

Y

S

D

I

F

E

E

Q

T

A

A

R

S

E

E

K

L

L

I

A

L

F

R

G

G

A

A

D

D

K

K

I

V

S
x
E

I

I

N

N

S

T

P

A

A

A

L

V

S

E

D

N

P

P

G

S

L

L

I

I

D

T

R

Q

L

I

Q

A

D

Q

E

T

F

F

G

G

R

S

Q

Q

C

A

I

V

V

V

I

V

G

-

I

-

D

-

S

-

Y

Y

Y

I

D

A

A

A

D

K

T

R

D

V

S

D

Y

G

K

E

V

F

K

M

Q

V

F

F

T

T

G

-

D

-

E

Y

A

S

A

G

T

T

K

K

D

N

T

T

Q

G

W

I

Y

L

T

L

Q

R

D

D

W

W

V

V

E

V

E

E

V

V

Q

E

R

K

R

R

G

G

C

A

G

G

E

E

I

I

V

V

L

L

N

G

V

S

M

I

N

D

Q

R

D

L

G

G

V

T

R

K

Q

S

G

G

Y

Y

D

D

I

T

K

E

Q

M

L

I

A

R

M

F

V

V

R

R

A

P

I

L

C

T

D

T

V

L

P

P

L

I

I

I

A

A

S

H

G

R

G

G

A

A

G

G

T

K

M

T

E

E

H

H

F

F

L

L

E

E

V

A

F

F

T

L

Q

-

A

A

G

G

V

A

D

D

A

A

L
x
K

A

A

A

D

S

S

V

V

F

F

H

H

K

S

G

R

I

E

I

I

E

D

I

V

E

R

A

E

L

L

K

K

R

E

Y

Y

L

L

L

K

D

K

N

H

G

G

V

V

Q

N

M

V

R

R

L

L

binding magnesium ion: E100 (≠ S101), K221 (≠ L229)

2wjzE Crystal structure of (hish) k181a y138a mutant of imidazoleglycerolphosphate synthase (hish hisf) which displays constitutive glutaminase activity (see paper)
47% identity, 100% coverage: 1:257/257 of query aligns to 1:236/237 of 2wjzE

query
sites
2wjzE

M

M

L

L

A

A

K

K

R

R

I

I

V

I

P

A

C

C

L

L

D
|
D

V

V

R

K

D

D

G

G

K

R

V

V

K

-

G

-

V

-

Q

-

F

-

R

-

N

-

H

-

E

-

I

-

V

-

G

G

D

D

I

P

V

V

P

E

L

L

A

G

A

K

R

F

Y

Y

A

S

A

E

E

I

S

G

A

I

D

D

E

E

L

L

V

V

F

F

Y

L

D

D

I

I

T

T

A

A

S

K

A

-

H

-

D

-

R

R

V

K

V

T

D

M

K

L

S

E

W

L

V

V

S

E

R

K

V

V

A

A

E

E

Q

Q

I

I

D

D

I

I

P

P

F

F

C

T

V

V

A

G

G

G

G
|
G

I

I

K

H

S

D

I

F

E

E

Q

T

A

A

R

S

E

E

K

L

L

I

A

L

F

R

G

G

A

A

D

D

K

K

I

V

S

S

I

I

N
|
N

S
x
T

P

A

A

A

L

V

S

E

D

N

P

P

G

S

L

L

I

I

D

T

R

Q

L

I

Q

A

D

Q

E

T

F

F

G

G

R

S

Q

Q

C

A

I

V

V

V

I

V

G

A

I

I

D
|
D

S

A

-

K

Y

R

Y

V

D

D

A

G

D

E

T

-

D

-

S

-

Y

F

K

M

V

V

K

F

Q

T

F

Y

T

S

G

G

D

K

E

-

A

-

T

-

K

K

D

N

T

T

Q

G

W

I

Y

L

T

L

Q

R

D

D

W

W

V

V

E

V

E

E

V

V

Q

E

R

K

R

R

G

G

C

A

G

G

E

E

I

I

V

L

L

L

N

T

V

S

M

I

N

D

Q

R

D

D

G

G

V

T

R

K

Q

S

G

G

Y

Y

D

D

I

T

K

E

Q

M

L

I

A

R

M

F

V

V

R

R

A

P

I

L

C

T

D

T

V

L

P

P

L

I

I

I

A

A

S

S

G

G

A

A

G

G

T

K

M

M

E

E

H

H

F

F

L

L

E

E

V

A

F

F

T

L

Q

A

A

G

G

-

V

A

D

D

A

A

L

L

A

A

S

S

V

V

F

F

H

H

K

F

G

R

I

E

I

I

E

D

I

V

E

R

A

E

L

L

K

K

R

E

Y

Y

L

L

L

K

D

K

N

H

G

G

V

V

Q

N

M

V

R

R

L

L

active site: D11 (= D11), D116 (= D130)
binding phosphate ion: G68 (= G82), N89 (= N103), T90 (≠ S104)

1h5yB Hisf protein from pyrobaculum aerophilum (see paper)
42% identity, 100% coverage: 2:257/257 of query aligns to 3:253/253 of 1h5yB

query
sites
1h5yB

L

M

A

A

K

L

R

R

I

I

V

I

P

P

C

C

L

L

D
|
D

V

I

R

D

D

G

G

G

K

A

-

K

-

V

V

V

K
|
K

G

G

V

V

Q

N

F

F

R

Q

N

G

H

I

E

R

I

E

V

V

G

G

D

D

I

P

V

V

P

E

L

M

A

A

A

V

R

R

Y

Y

A

E

E

E

S

G

A

A

D

D

E

E

L

I

V

A

F

I

Y

L

D

D

I

I

T

T

A

A

S

A

A

P

H

E

D

G

R

R

V

A

V

T

D

F

K

I

S

D

W

S

V

V

S

K

R

R

V

V

A

A

E

E

Q

A

I

V

D

S

I

I

P

P

F

V

C

L

V

V

A

G

G
|
G

I

V

K

R

S

S

I

L

E

E

Q

D

A

A

R

T

E

T

K

L

L

F

A

R

F

A

G

G

A

A

D

D

K

K

I

V

S

S

I

V

N
|
N

S
x
T

P

A

A

A

L

V

S

R

D

N

P

P

G

Q

L

L

I

V

D

A

R

L

L

L

Q

A

D

R

E

E

F

F

G

G

R

S

Q

Q

C

S

I

T

V

V

I

V

G
x
A

I

I

D
|
D

S

A

Y

K

Y

W

D

N

A

G

D

E

T

Y

D

-

S

-

Y

Y

K

E

V

V

K

Y

Q

V

F

K

T
x
G

G

G

D

R

E

E

A

A

A

T

T

G

K

L

D

D

T

-

Q

-

W

-

Y

-

T

A

Q

V

D

K

W

W

V

A

E

K

E

E

V

V

Q

E

R

E

R

L

G

G

C

A

G

G

E

E

I

I

V

L

L

L

N
x
T

V

S

M

I

N

D

Q

R

D
|
D

G
|
G

V

T

R

G

Q

L

G

G

Y

Y

D

D

I

V

K

E

Q

L

L

I

A

R

M

R

V

V

R

A

A

D

I

S

C

V

D

R

V

I

P

P

L

V

I

I

A

A

S

S

G

G

A

A

G

G

T

R

M

V

E

E

H

H

F

F

L

Y

E

E

V

A

F

A

T

A

Q

A

A

G

G

-

V

A

D

D

A

A

A

V

L

L

A
|
A

S
|
S

V

L

F

F

H

H

K

F

G

R

I

V

I

L

E

S

I

I

E

A

A

Q

L

V

K

K

R

R

Y

Y

L

L

L

K

D

E

N

R

G

G

V

V

Q

E

M

V

R

R

L

I

active site: D12 (= D11), D133 (= D130)
binding glycerol: K22 (= K19), N106 (= N103), A131 (≠ G128), D133 (= D130), G147 (≠ T146), T174 (≠ N177), D179 (= D182)
binding phosphate ion: G84 (= G81), G85 (= G82), N106 (= N103), T107 (≠ S104), D179 (= D182), G180 (= G183), A226 (= A230), A227 (= A231), S228 (= S232)

P33734 Imidazole glycerol phosphate synthase hisHF; IGP synthase; IGPS; ImGP synthase; EC 4.3.2.10; EC 3.5.1.2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
31% identity, 100% coverage: 2:257/257 of query aligns to 236:549/552 of P33734

query
sites
P33734

L

L

A

T

K

R

R
|
R

I

I

V

I

P

A

C

C

L

L

D

D

V

V

R

R

-

T

-

N

-

D

-

Q

-

G

D

D

G

L

K

V

V

V

V

T

K
|
K

G

G

V

D

Q

Q

F

Y

R

D

N

V

H

R

E

E

I

K

-

S

-

D

-

G

-

K

-

G

-

V

-

R

-

N

V

L

G

G

D

K

I

P

V

V

P

Q

L

L

A

A

A

Q

R

K

Y

Y

A

Y

A

Q

E

Q

S

G

A

A

D

D

E

E

L

V

V

T

F

F

Y

L

D

N

I

I

T

T

A

S

S

F

A

R

H

D

D

C

R

P

V

L

V

K

D

D

K

T

S

P

W

M

V

L

-

E

-

V

-

L

S

K

R

Q

V

A

A

A

E

K

Q

T

I

V

D

F

I

V

P

P

F

L

C

T

V

V

A

G

G

G

I

I

K

K

S

D

I

I

-

V

-

D

-

V

-

D

-

G

-

T

-

K

-

I

-

P

-

A

-

L

E

E

Q

V

A

A

R

S

E

L

K

Y

L

F

A

R

F

S

G

G

A

A

D

D

K
|
K

I

V

S

S

I

I

N

G

S

T

P

D

A

A

-

V

-

Y

-

A

-

E

-

K

-

Y

-

E

L

L

S

G

D

N

P

R

G

G

-

D

-

G

-

T

-

S

L

P

I

I

D

E

R

T

L

I

Q

S

D

K

E

A

F

Y

G

G

R

A

Q

Q

C

A

I

V

V

V

I

I

G

S

I

V

D

D

S

P

-

K

-

R

-

V

Y

Y

Y

V

D

N

A

S

D

Q

T

A

D

D

S

T

Y

-

K

K

V

N

K

K

Q

V

F

F

T

E

G

T

D

E

E

Y

A

P

A

G

T

P

K

N

D

G

T

E

Q

K

-

Y

-

C

W

W

Y

Y

-

Q

-

C

-

T

-

I

-

K

-

G

-

R

-

E

T

S

Q

R

D

D

W

L

-

G

V

V

E

W

E

E

V

L

Q

T

R

R

R

A

-

C

-

E

-

A

-

L

G

G

C

A

G

G

E

E

I

I

V

L

L

L

N

N

V

C

M

I

N

D

Q

K

D

D

G

G

V

S

R

N

Q

S

G

G

Y

Y

D

D

I

L

K

E

Q

L

L

I

A

E

M

H

V

V

R

K

A

D

I

A

C

V

D

K

V

I

P

P

L

V

I

I

A

A

S

S

G

S

G

G

A

A

G

G

T

V

M

P

E

E

H

H

F

F

L

E

E

E

V

A

F

F

T

L

Q

K

A

T

G

R

V

A

D

D

A

A

A

C

L

L

A

G

A

A

S

G

V

M

F

F

H

H

K

R

G

G

I

E

I

F

E

T

I

V

E

N

A

D

L

V

K

K

R

E

Y

Y

L

L

D

E

N

H

G

G

V

L

Q

K

M

V

R

R

L

M

R239 (= R5) mutation R->A,H,K: 1000-fold decrease in catalytic efficiency. Uncoupling of glutaminase activity from the cyclase activity.
K258 (= K19) mutation to A: No activity. Uncoupling of glutaminase activity from the cyclase activity.; mutation to R: Small reduction of activity.
K360 (= K99) mutation K->A,R: Almost no effect on activity.

1ox5A Towards understanding the mechanism of the complex cyclization reaction catalyzed by imidazole glycerophosphate synthase (see paper)
31% identity, 100% coverage: 2:257/257 of query aligns to 239:531/532 of 1ox5A

query
sites
1ox5A

L

L

A

T

K

R

R

R

I

I

V

I

P

A

C

C

L

L

D
|
D

V

V

R

R

D

T

G

N

K

D

V

Q

V

G

K

D

G

L

V

V

Q

V

F

T

R

K

N

-

H

-

E

-

I

-

V

L

G

G

D

K

I

P

V

V

P

Q

L

L

A

A

A

Q

R

K

Y

Y

A

Y

A

Q

E

Q

S

G

A

A

D

D

E

E

L

V

V

T

F

F

Y
x
L

D

N

I
|
I

T

T

A

C

S

P

A

L

H

K

D

D

R

T

V

P

V

M

D

L

K

E

S

V

W

-

V

L

S

K

R

Q

V

A

A

A

E

K

Q

T

I

V

D

F

I

V

P

P

F

L

C

T

V

V

A

G

G
|
G

G

G

I

I

K

K

S

D

I

I

-

V

-

D

-

V

-

D

-

G

-

T

-

K

-

I

-

P

-

A

-

L

E

E

Q

V

A

A

R

S

E

L

K

Y

L

F

A

R

F

S

G

G

A

A

D

D

K

K

I

V

S

S

I

I

N
x
G

S
x
T

P

D

A

A

-

V

-

Y

-

A

-

E

-

K

-

Y

-

E

L

L

S

G

D

N

P

R

G

G

-

D

-

G

-

T

-

S

L

P

I

I

D

E

R

T

L

I

Q

S

D

K

E

A

F

Y

G

G

R

A

Q

Q

C

A

I

V

V

V

I

I

G
x
S

I

V

D
|
D

S

P

-

K

-

R

-

V

Y

Y

Y

V

D

N

A

S

D

Q

T

A

D

D

S

T

Y

-

K

K

V

N

K

K

Q

V

F

F

T

E

G

T

D

E

E

Y

A

P

A

G

T

P

K

N

D

G

T

E

Q

K

-

Y

-

C

W

W

Y

Y

-

Q

-

C

-

T

-

I

-

K

-
x
G

-

G

-

R

-

E

T

S

Q

R

D

D

W

L

-

G

V

V

E

W

E

E

V

L

Q

T

R

R

R

A

-

C

-

E

-

A

-

L

G

G

C

A

G

G

E

E

I

I

V

L

L

L

N
|
N

V

C

M

I

N

D

Q

K

D
|
D

G
|
G

V

S

R

N

Q

S

G

G

Y

Y

D

D

I

L

K

E

Q

L

L

I

A

E

M

H

V

V

R

K

A

D

I

A

C

V

D

K

V

I

P

P

L

V

I

I

A

A

S

S

G
x
S

G

G

A

A

G

G

T

V

M

P

E

E

H

H

F

F

L

E

E

E

V

A

F

F

T

L

Q

K

A

T

G

R

V

A

D

D

A

A

A

C

L

L

A

G

A
|
A

S
x
G

V

M

F

F

H

H

K

R

G

G

I

E

I

F

E

T

I

V

E

N

A

D

L

V

K

K

R

E

Y

Y

L

L

D

E

N

H

G

G

V

L

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K

M

V

R

R

L

M

active site: D248 (= D11), D386 (= D130)
binding phosphoric acid mono-[5-({[5-carbamoyl-3-(5-phosphonooxy-5-deoxy-ribofuranosyl)- 3h-imidazol-4-ylamino]-methyl}-amino)-2,3,4-trihydroxy-pentyl] ester: L283 (≠ Y50), I285 (= I52), G313 (= G81), G346 (≠ N103), T347 (≠ S104), S384 (≠ G128), D386 (= D130), G424 (vs. gap), N451 (= N177), D456 (= D182), G457 (= G183), S482 (≠ G208), A505 (= A231), G506 (≠ S232)

1ox4B Towards understanding the mechanism of the complex cyclization reaction catalyzed by imidazole glycerophosphate synthase (see paper)
32% identity, 100% coverage: 2:257/257 of query aligns to 239:537/538 of 1ox4B

query
sites
1ox4B

L

L

A

T

K

R

R

R

I

I

V

I

P

A

C

C

L

L

D
|
D

V

V

R

R

-

T

-

N

-

D

-

Q

-

G

D

D

G

L

K

V

V

V

V

T

K

K

G

G

V

-

Q

-

F

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R

-

N

-

H

-

E

-

I

D

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G

D

K

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Q

L

L

A

A

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Y

Y

A

Y

A

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E

Q

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G

A

A

D

D

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E

L

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F

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N

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D

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-

E

-

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-

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A

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F

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-

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-

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D

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L

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E

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A

-

C

-

E

-

A

-

L

G

G

C

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G

G

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E

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I

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L

L

L

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N

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I

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G

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Y

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D

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L

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M

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R

K

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C

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P

L

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x
S

G

G

A

A

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G

T

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M

P

E

E

H

H

F

F

L

E

E

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A

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F

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L

Q

K

A

T

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R

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D

D

A

A

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C

L

L

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A
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x
G

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M

F

F

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K

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G

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I

F

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I

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E

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A

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Y

L

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V

R

R

L

M

active site: D248 (= D11), D392 (= D130)
binding pyrophosphate 2-: G463 (= G183), S488 (≠ G208), A511 (= A231), G512 (≠ S232)

3tdmA Computationally designed tim-barrel protein, halfflr (see paper)
42% identity, 38% coverage: 123:220/257 of query aligns to 1:92/120 of 3tdmA

query
sites
3tdmA

Q

Q

C

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I

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A

I

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-

D

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M

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G

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H

F

F

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L

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F

F

binding phosphate ion: D54 (= D182), G55 (= G183)

Sites not aligning to the query:

binding phosphate ion: 102, 103

5dn1A Crystal structure of phosphoribosyl isomerase a from streptomyces coelicolor (see paper)
24% identity, 92% coverage: 6:242/257 of query aligns to 6:233/240 of 5dn1A

query
sites
5dn1A

I

L

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L

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L

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D

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R

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D

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x
G

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x
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x
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x
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G

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x
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-

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x
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E

active site: D11 (= D11), D130 (= D130)
binding aminoimidazole 4-carboxamide ribonucleotide: G23 (≠ F23), L54 (≠ I52), F58 (≠ H57), S81 (≠ A80), G83 (= G82), R85 (≠ K84), G104 (≠ N103), T105 (≠ S104), G140 (≠ Q144), W141 (≠ F145)

P16250 Phosphoribosyl isomerase A; 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase; N-(5'-phosphoribosyl)anthranilate isomerase; PRAI; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; EC 5.3.1.16; EC 5.3.1.24 from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see paper)
24% identity, 92% coverage: 6:242/257 of query aligns to 6:233/240 of P16250

query
sites
P16250

I

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x
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G

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x
T

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D

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A

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A

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P

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L

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A

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G

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A

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A

A

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E

D11 (= D11) mutation to A: No activity.
R19 (≠ K19) mutation to A: No effect on activity toward PRA. No activity toward ProFAR.
S81 (≠ A80) mutation to T: No activity toward PRA. Almost no effect on activity toward ProFAR.
D130 (= D130) mutation to A: Very low activity toward PRA. No activity toward ProFAR.; mutation to Q: No activity.
T166 (≠ N177) mutation to A: No activity.
D171 (= D182) mutation to A: Low activity toward PRA. No activity toward ProFAR.

3zs4A Crystal structure of mycobacterium tuberculosis phosphoribosyl isomerase with bound prfar
25% identity, 89% coverage: 6:235/257 of query aligns to 5:229/244 of 3zs4A

query
sites
3zs4A

I

L

V

L

P

P

C
x
A

L

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D

V

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R

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R

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A

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x
R

G

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Q

F

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A

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A

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A
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x
F

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G

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D

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A

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T

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x
G

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W

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E

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D

-

G

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D

-

L

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W

D

D

W

V

V

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E

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S

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E

G

G

C

C

G

S

E

R

I

F

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V

L

V

N

T

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D

M

I

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T

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K

D
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D

G
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G

V

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R

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Q

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G

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Y

P

D

N

I

L

K

D

Q

L

L

L

A

A

M

G

V

V

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A

A

D

I

R

C

T

D

D

V

A

P

P

L

V

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I

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G
|
G

A

V

G

S

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S

M

L

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D

H

D

F

L

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R

-

A

L

I

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A

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F

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E

A

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A

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I

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Y

active site: D10 (= D11), D129 (= D130)
binding phosphoric acid mono-[5-({[5-carbamoyl-3-(5-phosphonooxy-5-deoxy-ribofuranosyl)- 3h-imidazol-4-ylamino]-methyl}-amino)-2,3,4-trihydroxy-pentyl] ester: A8 (≠ C9), D10 (= D11), R18 (≠ K19), A56 (= A56), F57 (≠ H57), S80 (≠ A80), G82 (= G82), R84 (≠ K84), G103 (≠ N103), T104 (≠ S104), D129 (= D130), G143 (≠ Q156), W144 (= W157), D174 (= D182), G175 (= G183), G200 (= G208), G201 (= G209), I223 (≠ L229), G225 (≠ A231), K226 (≠ S232)

2y88A Crystal structure of mycobacterium tuberculosis phosphoribosyl isomerase (variant d11n) with bound prfar (see paper)
24% identity, 89% coverage: 6:235/257 of query aligns to 5:229/244 of 2y88A

query
sites
2y88A

I

L

V

L

P

P

C
x
A

L

V

D
x
N

V

V

R

V

D

E

G

G

K

R

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A

V

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x
R

G

L

V

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active site: N10 (≠ D11), D129 (= D130)
binding [(2r,3s,4r,5r)-5-[4-aminocarbonyl-5-[[(z)-[(3r,4r)-3,4-dihydroxy-2-oxo-5-phosphonooxy-pentyl]iminomethyl]amino]imidazol-1-yl]-3,4-dihydroxy-oxolan-2-yl]methyl dihydrogen phosphate: A8 (≠ C9), N10 (≠ D11), R18 (≠ K19), G22 (≠ F23), L53 (≠ I52), A56 (= A56), S80 (≠ A80), G81 (= G81), G82 (= G82), R84 (≠ K84), G103 (≠ N103), T104 (≠ S104), D129 (= D130), G143 (≠ Q156), W144 (= W157), D174 (= D182), G175 (= G183), S199 (= S207), G200 (= G208), G225 (≠ A231), K226 (≠ S232)

Query Sequence

>5209750 FitnessBrowser__PV4:5209750
MLAKRIVPCLDVRDGKVVKGVQFRNHEIVGDIVPLAARYAAESADELVFYDITASAHDRV
VDKSWVSRVAEQIDIPFCVAGGIKSIEQAREKLAFGADKISINSPALSDPGLIDRLQDEF
GRQCIVIGIDSYYDADTDSYKVKQFTGDEAATKDTQWYTQDWVEEVQRRGCGEIVLNVMN
QDGVRQGYDIKQLAMVRAICDVPLIASGGAGTMEHFLEVFTQAGVDAALAASVFHKGIIE
IEALKRYLLDNGVQMRL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory