SitesBLAST
Comparing 5209834 FitnessBrowser__PV4:5209834 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P0A6T3 Galactokinase; Galactose kinase; EC 2.7.1.6 from Escherichia coli (strain K12) (see 2 papers)
50% identity, 98% coverage: 5:391/393 of query aligns to 5:379/382 of P0A6T3
- Y371 (= Y383) mutation to H: Displays relaxed substrate specificity since it gains kinase activity toward sugars as diverse as D-galacturonic acid, D-talose, L-altrose, and L-glucose. Also shows enhanced turnover of the small pool of sugars converted by the wild-type enzyme.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
6qjeA Structure of human galactokinase 1 bound with 4-{[2-(methylsulfonyl)- 1h-imidazol-1-yl]methyl}-1,3-thiazole
45% identity, 96% coverage: 13:391/393 of query aligns to 17:385/390 of 6qjeA
- binding beta-D-galactopyranose: E41 (≠ D37), H42 (= H38), D44 (= D40), Y45 (= Y41), G181 (= G183), M183 (= M185), D184 (= D186), Y234 (= Y235), G344 (= G346)
- binding 2-(1,3-benzoxazol-2-ylamino)spiro[1,6,7,8-tetrahydroquinazoline-4,1'-cyclohexane]-5-one: S77 (≠ D73), Y107 (= Y102), V127 (≠ I129), S129 (≠ G131), L133 (≠ K135), S139 (= S141), S140 (= S142), R226 (≠ K227)
- binding 4-[(2-methylsulfonylimidazol-1-yl)methyl]-1,3-thiazole: R103 (vs. gap), A176 (≠ V178), G177 (= G179)
7s4cA Crystal structure of inhibitor-bound galactokinase (see paper)
45% identity, 96% coverage: 13:391/393 of query aligns to 18:386/391 of 7s4cA
- binding alpha-D-galactopyranose: E42 (≠ D37), H43 (= H38), D45 (= D40), Y46 (= Y41), G182 (= G183), D185 (= D186), Y235 (= Y235), G345 (= G346)
- binding phosphate ion: G135 (= G136), G137 (= G138), S139 (= S140), S140 (= S141), S141 (= S142), Q171 (= Q172), H174 (≠ N175), H362 (≠ E363), H366 (≠ T367), E369 (≠ V370)
- binding 2-({(4R)-4-(2-chlorophenyl)-2-[(6-fluoro-1,3-benzoxazol-2-yl)amino]-6-methyl-1,4-dihydropyrimidine-5-carbonyl}amino)pyridine-4-carboxylic acid: M54 (≠ V49), D82 (vs. gap), R104 (vs. gap), W105 (= W99), Y108 (= Y102), L134 (≠ K135), S140 (= S141), L144 (= L145), K194 (≠ A195), L212 (= L213), D214 (vs. gap), L217 (= L217), A218 (≠ R218), V219 (≠ L219), L294 (= L294), F302 (≠ I302), L354 (= L355), S380 (≠ C385), Q381 (= Q386), A382 (≠ S387)
7s49A Crystal structure of inhibitor-bound galactokinase (see paper)
45% identity, 96% coverage: 13:391/393 of query aligns to 18:386/391 of 7s49A
- binding (4R)-2-[(1,3-benzoxazol-2-yl)amino]-4-(4-chloro-1H-pyrazol-5-yl)-4,6,7,8-tetrahydroquinazolin-5(1H)-one: S78 (≠ D73), D82 (vs. gap), R104 (vs. gap), W105 (= W99), Y108 (= Y102), V128 (≠ I129), L134 (≠ K135), S140 (= S141), S141 (= S142), L144 (= L145), R227 (≠ K227)
- binding alpha-D-galactopyranose: E42 (≠ D37), H43 (= H38), D45 (= D40), Y46 (= Y41), G182 (= G183), D185 (= D186), Y235 (= Y235)
- binding phosphate ion: G135 (= G136), G137 (= G138), S139 (= S140), S140 (= S141), S141 (= S142), Q171 (= Q172), H174 (≠ N175), H362 (≠ E363), H366 (≠ T367)
7rclA Crystal structure of adp-bound galactokinase (see paper)
45% identity, 96% coverage: 13:391/393 of query aligns to 18:386/391 of 7rclA
- binding adenosine-5'-diphosphate: T76 (≠ A71), S78 (≠ D73), W105 (= W99), Y108 (= Y102), G135 (= G136), G137 (= G138), S139 (= S140), S140 (= S141), S141 (= S142), L144 (= L145), H228 (≠ R228)
- binding alpha-D-galactopyranose: E42 (≠ D37), H43 (= H38), D45 (= D40), Y46 (= Y41), C181 (= C182), G182 (= G183), M184 (= M185), D185 (= D186), Y235 (= Y235)
- binding phosphate ion: H362 (≠ E363), H366 (≠ T367)
7ozxB Structure of human galactokinase 1 bound with azepan-1-yl(2,6- difluorophenyl)methanone
45% identity, 96% coverage: 13:391/393 of query aligns to 18:386/391 of 7ozxB
- binding beta-D-galactopyranose: E42 (≠ D37), H43 (= H38), D45 (= D40), Y46 (= Y41), G182 (= G183), M184 (= M185), D185 (= D186), Y235 (= Y235), G345 (= G346)
- binding 2-(1,3-benzoxazol-2-ylamino)spiro[1,6,7,8-tetrahydroquinazoline-4,1'-cyclohexane]-5-one: S78 (≠ D73), D82 (vs. gap), R104 (vs. gap), Y108 (= Y102), S130 (≠ G131), L134 (≠ K135), S140 (= S141), S141 (= S142), L144 (= L145)
- binding (azepan-1-yl)(2,6-difluorophenyl)methanone: S32 (≠ Y27)
Sites not aligning to the query:
6zgyA Structure of human galactokinase 1 bound with 2-(4-chlorophenyl)-n- (pyrimidin-2-yl)acetamide (see paper)
45% identity, 96% coverage: 13:391/393 of query aligns to 18:386/391 of 6zgyA
- binding beta-D-galactopyranose: E42 (≠ D37), H43 (= H38), D45 (= D40), Y46 (= Y41), G182 (= G183), M184 (= M185), D185 (= D186), Y235 (= Y235)
- binding 2-(1,3-benzoxazol-2-ylamino)spiro[1,6,7,8-tetrahydroquinazoline-4,1'-cyclohexane]-5-one: D82 (vs. gap), R104 (vs. gap), W105 (= W99), V128 (≠ I129), S130 (≠ G131), L134 (≠ K135), S140 (= S141), L144 (= L145)
- binding (2,5-dimethylphenyl) pyridine-4-carboxylate: L212 (= L213), D214 (vs. gap), L217 (= L217), L294 (= L294), F302 (≠ I302), L354 (= L355)
6q91A Structure of human galactokinase 1 bound with 5-chloro-n-isobutyl-2- methoxybenzamide
45% identity, 96% coverage: 13:391/393 of query aligns to 19:387/392 of 6q91A
- binding beta-D-galactopyranose: E43 (≠ D37), H44 (= H38), D46 (= D40), Y47 (= Y41), C182 (= C182), G183 (= G183), M185 (= M185), D186 (= D186), Y236 (= Y235), G346 (= G346)
- binding 2-(1,3-benzoxazol-2-ylamino)spiro[1,6,7,8-tetrahydroquinazoline-4,1'-cyclohexane]-5-one: R105 (vs. gap), W106 (= W99), Y109 (= Y102), S131 (≠ G131), L135 (≠ K135), G136 (= G136), S141 (= S141)
- binding 5-chloranyl-2-methoxy-~{N}-(2-methylpropyl)benzamide: L213 (= L213), S214 (≠ P214), D215 (vs. gap), L218 (= L217), L295 (= L294), G298 (= G297), D299 (= D298), Y300 (≠ N299), F303 (≠ I302), L355 (= L355)
6q3xA Structure of human galactokinase in complex with galactose and 2'- (benzo[d]oxazol-2-ylamino)-7',8'-dihydro-1'h-spiro[cyclohexane-1,4'- quinazolin]-5'(6'h)-one (see paper)
45% identity, 96% coverage: 13:391/393 of query aligns to 20:388/393 of 6q3xA
- binding beta-D-galactopyranose: E44 (≠ D37), H45 (= H38), D47 (= D40), G184 (= G183), M186 (= M185), D187 (= D186), Y237 (= Y235), G347 (= G346)
- binding 2-(1,3-benzoxazol-2-ylamino)spiro[1,6,7,8-tetrahydroquinazoline-4,1'-cyclohexane]-5-one: Y110 (= Y102), L136 (≠ K135), S142 (= S141), S143 (= S142), L146 (= L145)
6q3wC Structure of human galactokinase 1 bound with ethyl 1-(2-pyrazinyl)-4- piperidinecarboxylate
45% identity, 96% coverage: 13:391/393 of query aligns to 18:385/390 of 6q3wC
- binding beta-D-galactopyranose: E42 (≠ D37), H43 (= H38), D45 (= D40), Y46 (= Y41), G182 (= G183), M184 (= M185), D185 (= D186), Y235 (= Y235), G344 (= G346)
- binding 2-(1,3-benzoxazol-2-ylamino)spiro[1,6,7,8-tetrahydroquinazoline-4,1'-cyclohexane]-5-one: D82 (vs. gap), R104 (vs. gap), W105 (= W99), Y108 (= Y102), V128 (≠ I129), S130 (≠ G131), L134 (≠ K135), S140 (= S141), S141 (= S142), R227 (≠ K227)
- binding ethyl 1-pyrazin-2-ylpiperidine-4-carboxylate: R104 (vs. gap), N107 (= N101), Y108 (= Y102), A177 (≠ V178), G178 (= G179)
6q8zA Structure of human galactokinase 1 bound with n-(cyclobutylmethyl)-1, 5-dimethyl-1h-pyrazole-4-carboxamide
45% identity, 96% coverage: 13:391/393 of query aligns to 19:387/392 of 6q8zA
- binding beta-D-galactopyranose: E43 (≠ D37), H44 (= H38), D46 (= D40), Y47 (= Y41), G183 (= G183), M185 (= M185), D186 (= D186), Y236 (= Y235), G345 (= G345), G346 (= G346)
- binding 2-(1,3-benzoxazol-2-ylamino)spiro[1,6,7,8-tetrahydroquinazoline-4,1'-cyclohexane]-5-one: R105 (vs. gap), Y109 (= Y102), V129 (≠ I129), L135 (≠ K135), G136 (= G136), S141 (= S141), S142 (= S142)
- binding ~{N}-(cyclobutylmethyl)-1,5-dimethyl-pyrazole-4-carboxamide: A31 (≠ V25), V32 (≠ A26), S33 (≠ Y27)
Sites not aligning to the query:
6zh0A Structure of human galactokinase 1 bound with 2-(4-chlorophenyl)-n- (pyrimidin-2-yl)acetamide (see paper)
44% identity, 96% coverage: 13:391/393 of query aligns to 18:385/390 of 6zh0A
- binding beta-D-galactopyranose: R36 (= R31), E42 (≠ D37), H43 (= H38), D45 (= D40), G182 (= G183), M184 (= M185), D185 (= D186), Y235 (= Y235), G345 (= G346)
- binding 2-(1,3-benzoxazol-2-ylamino)spiro[1,6,7,8-tetrahydroquinazoline-4,1'-cyclohexane]-5-one: D82 (vs. gap), R104 (vs. gap), W105 (= W99), L134 (≠ K135), S140 (= S141), L144 (= L145)
- binding N-(3-chlorophenyl)-2,2,2-trifluoroacetamide: L212 (= L213), L294 (= L294), G297 (= G297), D298 (= D298), Y299 (≠ N299), F302 (≠ I302), L354 (= L355)
6q90C Structure of human galactokinase 1 bound with 1-(4-methoxyphenyl)-3- (4-pyridinyl)urea
45% identity, 96% coverage: 13:391/393 of query aligns to 19:386/391 of 6q90C
- binding beta-D-galactopyranose: E43 (≠ D37), H44 (= H38), D46 (= D40), Y47 (= Y41), G182 (= G183), D185 (= D186)
- binding 2-(1,3-benzoxazol-2-ylamino)spiro[1,6,7,8-tetrahydroquinazoline-4,1'-cyclohexane]-5-one: R104 (vs. gap), W105 (= W99), Y108 (= Y102), L134 (≠ K135), S140 (= S141), L144 (= L145)
- binding N-(4-methoxyphenyl)-N'-pyridin-4-ylurea: L212 (= L213), D214 (vs. gap), P215 (≠ A215), L217 (= L217), L354 (= L355), Q381 (= Q386)
6zfhA Structure of human galactokinase in complex with galactose and 2'- (benzo[d]oxazol-2-ylamino)-7',8'-dihydro-1'h-spiro[cyclopentane-1,4'- quinazolin]-5'(6'h)-one (see paper)
44% identity, 96% coverage: 13:391/393 of query aligns to 17:381/386 of 6zfhA
- binding beta-D-galactopyranose: E41 (≠ D37), H42 (= H38), D44 (= D40), G181 (= G183), M183 (= M185), D184 (= D186), Y230 (= Y235), G340 (= G346)
- binding 2-(1,3-benzoxazol-2-ylamino)spiro[1,6,7,8-tetrahydroquinazoline-4,1'-cyclopentane]-5-one: W104 (= W99), Y107 (= Y102), L133 (≠ K135), S139 (= S141), S140 (= S142), L143 (= L145)
1wuuA Crystal structure of human galactokinase complexed with mgamppnp and galactose (see paper)
44% identity, 96% coverage: 13:391/393 of query aligns to 20:386/391 of 1wuuA
- binding phosphoaminophosphonic acid-adenylate ester: T78 (≠ A71), S80 (≠ D73), W107 (= W99), Y110 (= Y102), L136 (≠ K135), G137 (= G136), G139 (= G138), S141 (= S140), S142 (= S141), S143 (= S142), L146 (= L145)
- binding alpha-D-galactopyranose: E44 (≠ D37), H45 (= H38), D47 (= D40), Y48 (= Y41), G184 (= G183), M186 (= M185), D187 (= D186), Y235 (= Y235)
6gr2A Structure of human galactokinase in complex with galactose and adp
44% identity, 96% coverage: 13:391/393 of query aligns to 16:378/383 of 6gr2A
- binding adenosine-5'-diphosphate: T74 (≠ A71), S76 (≠ D73), Y106 (= Y102), L132 (≠ K135), G133 (= G136), G135 (= G138), S137 (= S140), S138 (= S141), S139 (= S142)
- binding beta-D-galactopyranose: E40 (≠ D37), H41 (= H38), D43 (= D40), G180 (= G183), D183 (= D186), Y227 (= Y235)
6zgvD Structure of human galactokinase 1 bound with 2-(4-chlorophenyl)-n- (pyrimidin-2-yl)acetamide (see paper)
44% identity, 96% coverage: 13:391/393 of query aligns to 12:367/372 of 6zgvD
6zgwD Structure of human galactokinase 1 bound with (4-chlorophenyl)methyl pyridine-3-carboxylate (see paper)
44% identity, 96% coverage: 13:391/393 of query aligns to 13:366/371 of 6zgwD
6zgzD Structure of human galactokinase 1 bound with 2-(4-chlorophenyl)-n- (pyrimidin-2-yl)acetamide (see paper)
43% identity, 96% coverage: 13:391/393 of query aligns to 13:363/368 of 6zgzD
6zgxD Structure of human galactokinase 1 bound with 2-(4-chlorophenyl)-n- (pyrimidin-2-yl)acetamide (see paper)
43% identity, 94% coverage: 21:391/393 of query aligns to 11:354/359 of 6zgxD
Query Sequence
>5209834 FitnessBrowser__PV4:5209834
MKNGELTQALPYAFNQAFGQEAALVAYAPGRVNLIGDHTDYNLGWVLPVALDRGTSVAAS
QRDDSQIQVVARDMDNEKVSFSLDDQAMAQMPLDSVSPWSNYVRGTVKMLSAYLAETGQP
PLRGASLMISGNLPKGAGLSSSASLEMALIKAFAGLFDLKVDGIKAAQLGQQAENEFVGC
NCGIMDQLISAMGEAERAMLIDCSDLAIKQVPLPAGLRLMIINSNVKRTLVGSEYNLRRE
QCQQVAQHFGVSSLRQVSYEQLCRAEASLEPTLFRRARHVVSENARVLEMAEALSAGDNA
YIGRLMAASHRSLRDDFAVSTPEVDCLVELVSEVLGMDGGARMTGGGFGGCVVALLPEHK
IAEVRETIAVEYQARTGLTADIYLCQSGAGAFA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory