SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 5209835 FitnessBrowser__PV4:5209835 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 13 hits to proteins with known functional sites (download)

P09148 Galactose-1-phosphate uridylyltransferase; Gal-1-P uridylyltransferase; UDP-glucose--hexose-1-phosphate uridylyltransferase; EC 2.7.7.12 from Escherichia coli (strain K12) (see 4 papers)
58% identity, 95% coverage: 9:352/363 of query aligns to 2:346/348 of P09148

query
sites
P09148

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RAKR 28:31 (≠ RSKR 35:38) binding
C52 (= C59) binding ; mutation to S: Decreases enzyme activity 3000-fold.
C55 (= C62) binding ; mutation to S: Decreases enzyme activity 600-fold.
V61 (≠ I68) binding in other chain
ND 77:78 (= ND 84:85) binding in other chain
H115 (= H121) binding ; mutation to N: Decreases enzyme activity by 98%.
N153 (= N159) binding in other chain
GCS 159:161 (= GCS 165:167) binding in other chain
C160 (= C166) mutation C->S,A: Slight inhibition of enzymatic activity.
S161 (= S167) mutation to A: 7000-fold reduction in specific activity.
H164 (= H170) binding ; mutation to N: Decreases enzyme activity 10000-fold.
H166 (= H172) active site, Tele-UMP-histidine intermediate; mutation to G: Abolishes enzymatic activity.
Q168 (= Q174) binding in other chain
E182 (≠ K188) binding ; mutation to A: Decreases enzyme activity by about 50%. Abolishes iron binding, but has no effect on zinc binding.
H281 (= H287) binding
H296 (= H302) binding
H298 (= H304) binding
KF 311:312 (= KF 317:318) binding
YE 316:317 (= YE 322:323) binding
Q323 (= Q329) binding in other chain

1guqA Structure of nucleotidyltransferase complexed with udp-glucose (see paper)
58% identity, 95% coverage: 9:352/363 of query aligns to 1:345/347 of 1guqA

query
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1guqA

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active site: C51 (= C59), C54 (= C62), H114 (= H121), N152 (= N159), S160 (= S167), H163 (= H170), G165 (≠ H172), Q167 (= Q174)
binding fe (iii) ion: E181 (≠ K188), H280 (= H287), H295 (= H302), H297 (= H304)
binding potassium ion: E151 (= E158), N152 (= N159), K153 (= K160), G165 (≠ H172)
binding uridine-5'-diphosphate-glucose: R27 (= R35), R30 (= R38), W32 (= W40), F52 (= F60), V60 (≠ I68), N76 (= N84), D77 (= D85), F150 (= F157), N152 (= N159), G158 (= G165), C159 (= C166), S160 (= S167), Q167 (= Q174), K310 (= K317), F311 (= F318), Y315 (= Y322), E316 (= E323)
binding zinc ion: C51 (= C59), C54 (= C62), H114 (= H121), H163 (= H170)

1gupA Structure of nucleotidyltransferase complexed with udp-galactose (see paper)
58% identity, 95% coverage: 9:352/363 of query aligns to 1:345/347 of 1gupA

query
sites
1gupA

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active site: C51 (= C59), C54 (= C62), H114 (= H121), N152 (= N159), S160 (= S167), H163 (= H170), G165 (≠ H172), Q167 (= Q174)
binding fe (iii) ion: E181 (≠ K188), H280 (= H287), H295 (= H302), H297 (= H304)
binding galactose-uridine-5'-diphosphate: R27 (= R35), R30 (= R38), F52 (= F60), R59 (= R67), V60 (≠ I68), N76 (= N84), D77 (= D85), F78 (= F86), F150 (= F157), N152 (= N159), G158 (= G165), C159 (= C166), S160 (= S167), Q167 (= Q174), W169 (= W176), K310 (= K317), F311 (= F318), V313 (= V320), G314 (= G321), E316 (= E323)
binding potassium ion: N152 (= N159), K153 (= K160), G165 (≠ H172)
binding zinc ion: C51 (= C59), C54 (= C62), H114 (= H121), H163 (= H170)

1hxpA Nucleotide transferase (see paper)
57% identity, 95% coverage: 9:352/363 of query aligns to 1:338/340 of 1hxpA

query
sites
1hxpA

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active site: C44 (= C59), C47 (= C62), H107 (= H121), N145 (= N159), S153 (= S167), H156 (= H170), H158 (= H172), Q160 (= Q174)
binding beta-mercaptoethanol: N145 (= N159), C152 (= C166), Q160 (= Q174), C264 (= C278), S265 (≠ A279), L295 (= L309), A299 (= A313)
binding fe (iii) ion: E174 (≠ K188), H273 (= H287), H288 (= H302), H290 (= H304)
binding uridine-5'-monophosphate: F45 (= F60), V53 (≠ I68), N69 (= N84), D70 (= D85)
binding zinc ion: C44 (= C59), C47 (= C62), H107 (= H121), H156 (= H170)

1hxpB Nucleotide transferase (see paper)
56% identity, 95% coverage: 9:352/363 of query aligns to 1:329/329 of 1hxpB

query
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active site: C35 (= C59), C38 (= C62), H98 (= H121), N136 (= N159), S144 (= S167), H147 (= H170), H149 (= H172), Q151 (= Q174)
binding beta-mercaptoethanol: F3 (= F11), P5 (≠ T13), H10 (= H18), N136 (= N159), C143 (= C166), Q151 (= Q174), Y208 (= Y231)
binding fe (iii) ion: E165 (≠ K188), H264 (= H287), H279 (= H302), H281 (= H304)
binding uridine-5'-diphosphate: R43 (= R67), V44 (≠ I68), F58 (= F82), N60 (= N84), D61 (= D85), S144 (= S167), N145 (= N168)
binding zinc ion: C35 (= C59), C38 (= C62), H98 (= H121), H147 (= H170)

P07902 Galactose-1-phosphate uridylyltransferase; Gal-1-P uridylyltransferase; UDP-glucose--hexose-1-phosphate uridylyltransferase; EC 2.7.7.12 from Homo sapiens (Human) (see 22 papers)
52% identity, 93% coverage: 11:349/363 of query aligns to 24:366/379 of P07902

query
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P07902

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I32 (≠ R19) to N: in GALAC1; mild; dbSNP:rs111033644
Y34 (≠ F21) to N: in GALAC1; affects protein stability; dbSNP:rs111033836
V44 (≠ L31) to M: in GALAC1; reduced enzyme activity; dbSNP:rs111033647
L62 (= L49) to M: in dbSNP:rs1800461
L74 (= L61) to P: in GALAC1; reduced enzyme activity; dbSNP:rs111033663
H132 (≠ S118) to Q: in GALAC1; affects protein stability; dbSNP:rs367543256
S135 (≠ H121) to L: in GALAC1; about 5% of normal galactose uridylyltransferase activity; dbSNP:rs111033690
T138 (= T124) to M: in GALAC1; mild; dbSNP:rs111033686
M142 (≠ L128) to K: in GALAC1; 4% of normal activity; dbSNP:rs111033695
R148 (≠ Q134) to W: in GALAC1; unstable protein; dbSNP:rs111033693
V151 (≠ I137) to A: in GALAC1; approximately 3% of normal activity; dbSNP:rs111033701
V168 (= V154) to L: in GALAC1; loss of activity; dbSNP:rs367543258
I170 (≠ V156) to T: in GALAC1; loss of activity; dbSNP:rs111033839
F171 (= F157) to S: in GALAC1; reduced enzyme activity; dbSNP:rs111033715
G175 (= G161) to D: in GALAC1; strongly reduces galactose uridylyltransferase activity; dbSNP:rs111033718
P185 (= P171) to H: in GALAC1; loss of activity; dbSNP:rs111033722
H186 (= H172) active site, Tele-UMP-histidine intermediate
Q188 (= Q174) to R: in GALAC1; most common mutation; 10% of normal galactose uridylyltransferase activity; impairs protein folding; dbSNP:rs75391579
L195 (= L181) to P: in GALAC1; no enzymatic activity; dbSNP:rs111033728
R201 (≠ T187) to C: in GALAC1; 2-fold decrease in activity; dbSNP:rs111033739
E202 (≠ K188) binding
E220 (≠ D206) to K: in GALAC1; 3-fold decrease in activity; dbSNP:rs111033747
R223 (≠ E209) to S: in GALAC1; 3-fold decrease in activity; dbSNP:rs111033750
L227 (≠ Q213) to P: in GALAC1; results in no detectable protein in the soluble fraction; dbSNP:rs111033846
R231 (= R217) to H: in GALAC1; 15% of normal activity; dbSNP:rs111033754
R259 (≠ F245) to Q: in GALAC1; loss of activity; dbSNP:rs886042070; to W: in GALAC1; mild; dbSNP:rs786204763
I278 (= I264) to N: in GALAC1; 18-fold decrease in activity; dbSNP:rs111033778
K285 (≠ R271) to N: in GALAC1; severe; impairs protein folding; nearly abolishes enzyme activity; dbSNP:rs111033773
L289 (= L275) to F: in GALAC1; 3-fold decrease in activity; dbSNP:rs111033774
E291 (≠ Q277) to V: in GALAC1; 2-fold decrease in activity; dbSNP:rs111033841
H301 (= H287) binding
N314 (≠ P297) to D: in GALAC1; allele Duarte; exists as allelic variants Duarte-1 and Duarte-2; Duarte-1 has normal or increased activity; Duarte-2 has activity reduced to about 35-45% of normal; dbSNP:rs2070074
H319 (= H302) binding
H321 (= H304) binding
L327 (= L310) to P: in GALAC1; results in no detectable protein in the soluble fraction; dbSNP:rs111033832
A330 (= A313) to V: in GALAC1; mild; dbSNP:rs111033804
R333 (≠ K316) to W: in GALAC1; no enzymatic activity; dbSNP:rs111033800
T350 (= T333) to A: in GALAC1; mild; dbSNP:rs111033817

6gqdA Structure of human galactose-1-phosphate uridylyltransferase (galt), with crystallization epitope mutations a21y:a22t:t23p:r25l
51% identity, 93% coverage: 11:349/363 of query aligns to 4:342/344 of 6gqdA

query
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G

G

P

E

S

D

D

-

H

P

P

L

L

F

F

R

Q

F

A

E

K

T

S

V

A

E

R

G

G

E

V

S

C

R

K

V

V

L

M

C

C

F

F

S

H

P

P

D

W

H
x
S

S

D

L

V

T

T

L

L

A

P

Q

L

L

M

D

S

P

V

E

P

A

E

M

I

Q

R

A

A

V

V

I

V

A

D

A

A

W

W

Q

A

S

S

Q

V

S

T

E

E

L

E

L

L

G

G

K

A

K

Q

Y

Y

L

P

W

W

V

V

Q

Q

V

I

F

F

E

E

N
|
N

K

K

G

G

A

A

A

M

M

M

G

G

C

C

S
|
S

N

N

P

P

H
|
H

P

P

H
|
H

G

C

Q
|
Q

I

V

W

W

A

A

H

S

N

S

H

F

L

L

P

P

T

D

L

I

V

A

A

Q

T

R

K
x
E

Q

E

Q

R

R

S

L

Q

A

Q

D

A

Y

Y

Y

K

Q

S

Q

Q

H

H

H

G

S

E

N

P

L

L

G

M

D

E

Y

Y

V

S

E

R

R

Q

E

E

L

L

Q

L

Q

R

E

K

V

E

R

R

I

L

V

V

V

L

S

T

N

S

H

E

D

H

W

W

V

L

A

V

L

L

V

V

P

P

Y

F

W

W

A

A

S

T

W

W

P

P

F

Y

E

Q

T

T

L

L

V

L

L

L

P

P

R

R

F

R

D

H

I

V

R

R

M

L

T

P

E

E

L

L

T

T

A

P

E

A

T

E

R

R

E

D

S

D

L

L

G

A

E

S

I

I

I

M

G

K

A

K

L

L

T

L

V

T

R

K

Y

Y

D

D

N

N

L

L

F

F

Q

E

C

T

A

S

F

F

P

P

Y

Y

S

S

M

M

G

G

W

W

H
|
H

G

G

A

A

P

P

F

T

D

G

-

S

-

E

-

A

G

G

Q

A

D

N

H

W

P

D

E

H

W

W

C

Q

L

L

H
|
H

A

A

H
|
H

F

Y

Y

Y

P

P

L

L

R

R

S

S

A

A

S

T

V

V

K

R

K

K

F

F

M

M

V

V

G

G

Y

Y

E

E

L

M

L

L

A

A

E

Q

I

A

Q

Q

R

R

D

D

I

L

T

T

P

P

E

E

Q

Q

A

A

A

E

R

R

L

L

R

R

E

A

Q

L

S

P

P

E

I

V

H

H

Y

Y

active site: N48 (≠ C59), C51 (= C62), S111 (≠ H121), N149 (= N159), S157 (= S167), H160 (= H170), H162 (= H172), Q164 (= Q174)
binding 5,6-dihydrouridine-5'-monophosphate: P49 (≠ F60), A57 (≠ I68), N73 (= N84), D74 (= D85), H162 (= H172), Q164 (= Q174)
binding zinc ion: E178 (≠ K188), H277 (= H287), H295 (= H302), H297 (= H304)

5in3A Crystal structure of glucose-1-phosphate bound nucleotidylated human galactose-1-phosphate uridylyltransferase (see paper)
49% identity, 92% coverage: 15:349/363 of query aligns to 1:324/324 of 5in3A

query
sites
5in3A

E

D

H

H

P

Q

H

H

R

I

R

R

F

Y

N

N

P

P

L

L

T

Q

G

D

E

E

W

W

V

V

L

L

L

V

S

S

P

A

H

H

R

R

S

-

K

-

R

-

P

-

W

-

Q

-

G

-

Q

-

T

-

E

-

A

-

L

-

D

-

L

-

T

-

T

T

A

V

P

P

S

R

Y

H

D

D

S

P

E

L

C
x
N

F
x
P

L

L

C
|
C

P

P

G

G

N

A

K

I

R

R

I
x
A

N

N

G

G

E

E

Q

V

N

N

P

P

K

Q

Y

Y

D

D

T

S

T

T

F

F

V

L

F
|
F

Q

D

N
|
N

D
|
D

F

F

A

P

A

A

L

L

N

Q

S

P

D

D

G

A

P

P

K

S

A

P

Q

G

G

P

E

S

D

D

-

H

P

P

L

L

F

F

R

Q

F

A

E

K

T

S

V

A

E

R

G

G

E

V

S

C

R

K

V
|
V

L

M

C

C

F

F

S

H

P

P

D

W

H
x
S

S

D

L

V

T

T

L

L

A

P

Q

L

L

M

D

S

P

V

E

P

A

E

M

I

Q

R

A

A

V

V

I

V

A

D

A

A

W

W

Q

A

S

S

Q

V

S

T

E

E

L

E

L

L

G

G

K

A

K

Q

Y

Y

L

P

W

W

V

V

Q

Q

V

I

F
|
F

E

E

N
|
N

K

K

G

G

A

A

A

M

M

M

G

G

C

C

S
|
S

N

N

P

P

H
|
H

P

P

H
|
H

G

C

Q
|
Q

I

V

W

W

A

A

H

S

N

S

H

F

L

L

P

P

T

D

L

I

V

A

A

Q

T

R

K
x
E

Q

E

Q

R

R

S

L

Q

A

Q

D

A

Y

Y

Y

K

Q

S

Q

Q

H

H

H

G

S

E

N

P

L

L

G

M

D

E

Y

Y

V

S

E

R

R

Q

E

E

L

L

Q

L

Q

R

E

K

V

E

R

R

I

L

V

V

V

L

S

T

N

S

H

E

D

H

W

W

V

L

A

V

L

L

V

V

P

P

Y

F

W

W

A

A

S

T

W

W

P

P

F

Y

E

Q

T

T

L

L

V

L

L

L

P

P

R

R

F

R

D

H

I

V

R

R

M

L

T

P

E

E

L

L

T

T

A

P

E

A

T

E

R

R

E

D

S

D

L

L

G

A

E

S

I

I

I

M

G

K

A

K

L

L

T

L

V

T

R

K

Y

Y

D

D

N

N

L

L

F

F

Q

E

C

T

A

S

F

F

P

P

Y

Y

S

S

M

M

G

G

W

W

H
|
H

G

G

A

A

P

P

F

T

D

G

-

S

-

E

-

A

G

G

Q

A

D

N

H

W

P

N

E

H

W

W

C

Q

L

L

H
|
H

A

A

H
|
H

F

Y

Y

Y

P

P

L

L

R

R

S

S

A

A

S

T

V

V

K

R

K

K

F

F

M

M

V
|
V

G
|
G

Y

Y

E
|
E

L

M

L

L

A

A

E

Q

I

A

Q

Q

R

R

D

D

I

L

T

T

P

P

E

E

Q

Q

A

A

A

E

R

R

L

L

R

R

E

A

Q

L

S

P

P

E

I

V

H

H

Y

Y

active site: N30 (≠ C59), C33 (= C62), S93 (≠ H121), N131 (= N159), S139 (= S167), H142 (= H170), H144 (= H172), Q146 (= Q174)
binding 1-O-phosphono-alpha-D-glucopyranose: F129 (= F157), N131 (= N159), Q146 (= Q174), V295 (= V320), G296 (= G321), E298 (= E323)
binding 5,6-dihydrouridine-5'-monophosphate: P31 (≠ F60), A39 (≠ I68), F53 (= F82), N55 (= N84), D56 (= D85), V86 (= V114), H144 (= H172), Q146 (= Q174)
binding zinc ion: E160 (≠ K188), H259 (= H287), H277 (= H302), H279 (= H304)

6k5zB Structure of uridylyltransferase (see paper)
31% identity, 89% coverage: 20:342/363 of query aligns to 4:306/314 of 6k5zB

query
sites
6k5zB

R

R

F

K

N

D

P

P

L

F

T

T

G

G

E

E

W

Y

V

I

L

L

L

V

S

S

P

P

H

H

R

R

S

L

K

K

R

R

P

P

W

W

Q

Q

G

P

Q

-

T

-

E

-

A

-

L

-

D

-

L

-

T

-

A

-

P

-

S

-

Y

-

D

E

S

G

E

A

C
|
C

F

P

L

F

C
|
C

P

P

G

G

N

A

K

P

R

-

I

-

N

-

G

-

E

E

Q

T

N

G

P

R

K

G

Y

W

D

D

T

V

T

-

F

L

V

I

F

L

Q

P

N

N

D

R

F

Y

A

P

A

V

L

V

N

T

S

E

D

N

G

P

P

P

K

E

A

P

Q

T

G

A

E

E

D

D

P

-

L

L

F

Y

R

E

F

V

E

I

T

P

V

A

E

R

G

G

E

S

S

S

R

L

V

V

L

V

C

V

F

E

S

T

P

P

D

Q

H
|
H

S

D

-

V

-

D

-

L

-

S

-

D

L

L

T

P

L

L

A

G

Q

Q

L

I

D

-

P

-

E

K

A

K

M

I

Q

L

A

T

V

A

I

V

A

A

A

E

W

A

Q

Q

S

R

Q

K

S

A

E

E

L

K

L

E

G

G

K

N

K

A

Y

A

L

Y

W

F

V

L

Q

-

V

F

F

F

E

R

N
|
N

K

K

G

G

A

K

A

E

M

I

G

G

C
x
V

S
|
S

N
x
L

P

T

H
|
H

P

P

H
|
H

G

S

Q
|
Q

I

I

W

Y

A

I

H

L

N

P

H

V

L

V

P

P

T

P

L

R

V

V

A

R

T

A

K
x
E

Q

L

Q

Q

R

A

L

S

A

Y

D

E

Y

W

Y

Y

Q

V

Q

K

H

H

H

G

S

S

N
x
C

L

L

L

H

G
x
C

D

R

Y

I

V

V

E

E

R

K

E

E

L

-

Q

-

E

E

V

K

R

R

I

L

V

V

V

F

S

Q

N

N

H

R

D

N

W

W

V

K

A

A

L

F

V

V

P

P

Y

F

W

Y

A

A

S

K

W

W

P

P

F

H

E

E

T

V

L

H

V

I

L

Y

P

P

R

K

F

R

D
x
H

I

R

R

S

R

L

M

L

T

T

E

E

L

L

T

T

A

D

E

E

T

E

R

V

E

A

S

D

L

L

G

A

E

E

I

A

I

L

G

K

A

I

L

T

T

L

V

C

R

A

Y

L

D

K

N

Q

L

V

F

A

Q

G

C

I

A

P

F

M

P

P

Y

Y

S

I

M

M

G

V

W

L

H
|
H

G

Q

A

A

P

P

F

L

D

-

G

-

Q

-

D

P

H

R

P

P

E

T

W

Q

C

Y

L

Y

H
|
H

A

L

H
|
H

F

F

-
x
E

Y

I

P

Y

P

G

L

M

L

Y

R

R

S

P

A

D

S

G

V

K

K

L

K

K

F

H

M

A

V

A

G

G

Y

A

E

E

L

L

L

G

A

A

E

S

I

L

-

F

Q

T

R

L

D

D

I

T

T

T

P

P

E

E

Q

E

A

T

A

A

R

R

L

I

R

K

active site: C30 (= C59), C33 (= C62), H86 (= H121), N127 (= N159), S135 (= S167), H138 (= H170), H140 (= H172), Q142 (= Q174)
binding fe (iii) ion: E156 (≠ K188), H252 (= H287), H266 (= H304), E268 (vs. gap)
binding phosphate ion: V134 (≠ C166), S135 (= S167), L136 (≠ N168), H140 (= H172)
binding zinc ion: C30 (= C59), C33 (= C62), H86 (= H121), H138 (= H170), C170 (≠ N202), C173 (≠ G205), H211 (≠ D246), H264 (= H302)

6k9zA Structure of uridylyltransferase mutant (see paper)
28% identity, 89% coverage: 20:342/363 of query aligns to 4:299/309 of 6k9zA

query
sites
6k9zA

R

R

F

K

N

D

P

P

L

F

T

T

G

G

E

E

W

Y

V

I

L

L

L

V

S

S

P

P

H

Q

R

P

S

-

K

-

R

-

P

-

W

-

Q

-

G

-

Q

-

T

-

E

-

A

-

L

-

D

-

L

-

T

-

A

-

P

-

S

-

Y

-

D

E

S

G

E

A

C
|
C

F
x
P

L

F

C
|
C

P

P

G

G

N

A

K

P

R

-

I

-

N

-

G

-

E

E

Q

T

N

G

P

R

K

G

Y

W

D

D

T

V

T

-

F

L

V

I

F

L

Q

P

N
|
N

D
x
R

F
x
Y

A

P

A

V

L

V

N

T

S

E

D

N

G

P

P

P

K

E

A

P

Q

T

G

A

E

E

D

D

P

-

L

L

F

Y

R

E

F

V

E

I

T

P

V

A

E

R

G

G

E

S

S

S

R

L

V

V

L

V

C

V

F

E

S

T

P

P

D

Q

H
|
H

S

D

-

V

-

D

-

L

-

S

-

D

L

L

T

P

L

L

A

G

Q

Q

L

I

D

-

P

-

E

K

A

K

M

I

Q

L

A

T

V

A

I

V

A

A

A

E

W

A

Q

Q

S

R

Q

K

S

A

E

E

L

K

L

E

G

G

K

N

K

A

Y

A

L

Y

W

F

V

L

Q

-

V

F

F

F

E

R

N
|
N

K

K

G

G

A

K

A

E

M

I

G

G

C

V

S
|
S

N
x
L

P

T

H
|
H

P

P

H
x
F

G

S

Q
|
Q

I

I

W
x
Y

A

I

H

L

N

P

H

V

L

V

P

P

T

P

L

R

V

V

A

R

T

A

K
x
E

Q

L

Q

Q

R

A

L

S

A

Y

D

E

Y

W

Y

Y

Q

V

Q

K

H

H

H

G

S

S

N
x
C

L

L

L

H

G
x
C

D

R

Y

I

V

V

E

E

R

K

E

E

L

-

Q

-

E

E

V

K

R

R

I

L

V

V

V

F

S

Q

N

N

H

R

D

N

W

W

V

K

A

A

L

F

V

V

P

P

Y

F

W

Y

A

A

S

K

W

W

P

P

F

H

E

E

T

V

L

H

V

I

L

Y

P

P

R

K

F

R

D
x
H

I

R

R

S

R

L

M

L

T

T

E

E

L

L

T

T

A

D

E

E

T

E

R

V

E

A

S

D

L

L

G

A

E

E

I

A

I

L

G

K

A

I

L

T

T

L

V

C

R

A

Y

L

D

K

N

Q

L

V

F

A

Q

G

C

I

A

P

F

M

P

P

Y

Y

S

I

M

M

G

V

W

L

H
|
H

G

Q

A

A

P

P

F

L

D

-

G

-

Q

-

D

P

H

R

P

P

E

T

W

Q

C

Y

L

Y

H
|
H

A

L

H
|
H

F

F

-
x
E

Y

I

P

Y

P

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M

L

Y

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A

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A

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A

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G

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A

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E

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A

A

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-

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active site: C23 (= C59), C26 (= C62), H79 (= H121), N120 (= N159), S128 (= S167), H131 (= H170), F133 (≠ H172), Q135 (= Q174)
binding fe (iii) ion: E149 (≠ K188), H245 (= H287), H259 (= H304), E261 (vs. gap)
binding uridine-5'-diphosphate: P24 (≠ F60), N43 (= N84), R44 (≠ D85), Y45 (≠ F86), L129 (≠ N168), Q135 (= Q174), Y137 (≠ W176)
binding zinc ion: C23 (= C59), C26 (= C62), H79 (= H121), H131 (= H170), C163 (≠ N202), C166 (≠ G205), H204 (≠ D246), H257 (= H302)

Q9FK51 ADP-glucose phosphorylase; ADP-glucose:phosphate adenylyltransferase; EC 2.7.7.- from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
25% identity, 64% coverage: 14:244/363 of query aligns to 20:253/351 of Q9FK51

query
sites
Q9FK51

D

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RAKR 41:44 (≠ RSKR 35:38) binding
C63 (= C59) binding
C66 (= C62) binding
ECA 72:74 (≠ ING 68:70) binding
N94 (= N84) binding
H133 (= H121) binding
N173 (= N159) binding
GASM 179:182 (≠ GCSN 165:168) binding
H184 (= H170) binding
H186 (= H172) active site, Tele-AMP-histidine intermediate
Q188 (= Q174) binding
C216 (≠ Y207) binding
C219 (≠ R210) binding

Sites not aligning to the query:

255 binding
310 binding
321 binding
325:326 binding

2h39B Crystal structure of an adp-glucose phosphorylase from arabidopsis thaliana with bound adp-glucose
25% identity, 63% coverage: 15:244/363 of query aligns to 1:215/313 of 2h39B

query
sites
2h39B

E

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active site: C32 (= C59), C35 (= C62), H95 (= H121), N135 (= N159), S143 (= S167), H146 (= H170), G148 (≠ H172), Q150 (= Q174)
binding adenosine-5'-diphosphate-glucose: R21 (= R35), R24 (= R38), F34 (≠ L61), C42 (≠ N69), N63 (= N84), L64 (≠ D85), Y65 (≠ F86), F133 (= F157), N135 (= N159), G141 (= G165), A142 (≠ C166), S143 (= S167), M144 (≠ N168), Q150 (= Q174)
binding zinc ion: C32 (= C59), H95 (= H121), H146 (= H170), C178 (≠ Y207), C181 (≠ R210)

Sites not aligning to the query:

1z84A X-ray structure of galt-like protein from arabidopsis thaliana at5g18200 (see paper)
24% identity, 63% coverage: 17:244/363 of query aligns to 2:213/311 of 1z84A

query
sites
1z84A

P

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active site: C31 (= C59), C34 (= C62), H93 (= H121), N133 (= N159), S141 (= S167), H144 (= H170), H146 (= H172), Q148 (= Q174)
binding adenosine monophosphate: F33 (≠ L61), N62 (= N84), L63 (≠ D85), Y64 (≠ F86), N133 (= N159), A140 (≠ C166), S141 (= S167), M142 (≠ N168), H146 (= H172), Q148 (= Q174)
binding zinc ion: C31 (= C59), C34 (= C62), H93 (= H121), H144 (= H170), C176 (≠ Y207), C179 (≠ R210)

Sites not aligning to the query:

binding zinc ion: 215, 270

Query Sequence

>5209835 FitnessBrowser__PV4:5209835
MFQKTQAKSQFSTDEHPHRRFNPLTGEWVLLSPHRSKRPWQGQTEALDLTTAPSYDSECF
LCPGNKRINGEQNPKYDTTFVFQNDFAALNSDGPKAQGEDPLFRFETVEGESRVLCFSPD
HSLTLAQLDPEAMQAVIAAWQSQSELLGKKYLWVQVFENKGAAMGCSNPHPHGQIWAHNH
LPTLVATKQQRLADYYQQHHSNLLGDYVERELQQEVRIVVSNHDWVALVPYWASWPFETL
VLPRFDIRRMTELTAETRESLGEIIGALTVRYDNLFQCAFPYSMGWHGAPFDGQDHPEWC
LHAHFYPPLLRSASVKKFMVGYELLAEIQRDITPEQAAARLREQSPIHYKSSHYKNSHNK
QSQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory