SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 5209977 Shew_2425 multifunctional fatty acid oxidation complex subunit alpha (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6yswA E. Coli anaerobic trifunctional enzyme subunit-alpha in complex with coenzyme a
59% identity, 100% coverage: 4:706/706 of query aligns to 3:704/707 of 6yswA

query
sites
6yswA

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active site: A66 (= A67), I71 (≠ L72), A84 (≠ S85), Q88 (≠ H89), G112 (= G113), E115 (= E116), P136 (= P137), E137 (= E138), G145 (= G146), D264 (≠ E268), S422 (= S427), H443 (= H448), E455 (= E460), N493 (= N498)
binding coenzyme a: E23 (= E24), M25 (= M26), T27 (= T28), A64 (= A65), A66 (= A67), D67 (= D68), I68 (= I69), N69 (≠ S70), P136 (= P137), E137 (= E138), L140 (= L141), F286 (= F290), F287 (= F291), T290 (= T294), K293 (= K297)

P40939 Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Homo sapiens (Human) (see 5 papers)
44% identity, 98% coverage: 14:706/706 of query aligns to 49:763/763 of P40939

query
sites
P40939

I

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F

G

G

E

E

R

R

F

F

K

G

A

G

-

G

-

N

P

P

A

E

A

L

F

L

D

T

K

Q

L

M

L

V

A

S

D

K

D

G

R

F

K

L

G

G

R

R

K

K

N

S

G

G

K

K

G

G

F

F

Y

Y

Q

I

Y

Y

G

Q

A

E

K

G

S

V

K

K

K

R

K

K

L

D

V

L

D

N

-

S

-

D

-

M

E

D

S

S

V

I

Y

L

G

A

V

S

L

L

G

K

L

L

T

P

P

P

G

K

A

S

D

E

-

V

-

S

-

S

G

D

E

E

P

D

I

I

A

Q

L

F

A

-

E

-

R

R

C

L

V

V

V

T

Q

R

M

F

L

V

N

N

E

E

A

A

V

V

R

M

C

C

L

L

E

Q

E

E

G

G

I

I

I

L

A

A

S

T

P

P

R

A

D

E

G

G

D

D

I

I

G

G

A

A

I

V

F

F

G

G

I

L

G

G

F

F

P

P

P

P

F

C

L

L

G

G

G

G

P

P

F

F

R

R

Y

F

M

V

D

D

S

L

L

Y

G

G

A

A

K

Q

H

K

L

I

V

V

E

D

T

R

L

L

K

K

R

K

Y

Y

Q

E

D

A

Q

A

F

Y

G

G

D

K

R

Q

F

F

A

T

P

P

C

C

D

Q

R

L

L

L

V

A

E

D

M

H

A

A

E

N

S

S

-

P

G

N

S

K

K

K

F

F

Y

Y

E

Q

V282 (≠ I229) to D: in MTPD; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852773
I305 (= I252) to N: in MTPD; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852774
L342 (≠ I289) to P: in LCHAD deficiency; dbSNP:rs137852772
E510 (= E460) active site, For hydroxyacyl-coenzyme A dehydrogenase activity; to Q: in AFLP and LCHAD deficiency; loss of long-chain-3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137852769

1wdlA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form ii (native4) (see paper)
40% identity, 100% coverage: 3:705/706 of query aligns to 6:714/715 of 1wdlA

query
sites
1wdlA

K

K

S

A

F

I

T

T

L

V

N

T

R

A

L

L

D

E

N

S

G

G

I

I

A

V

V

E

L

L

T

K

M

F

D

D

V

L

P

K

G

G

E

E

T

S

M

V

N

N

T

K

L

F

R

N

A

R

E

L

F

T

G

L

P

N

E

E

I

L

C

R

E

Q

M

A

L

V

A

D

E

A

I

I

K

K

A

A

D

D

A

A

G

S

I

V

R

K

G

G

V

V

V

I

I

V

I

S

S

S

G

G

K

K

K

-

D

D

S

V

F

F

V

I

A

V

G

G

A
|
A

D

D

I

I

S

T

M

E

L

F

D

V

A

E

C

N

A

F

T

K

A

L

E

P

D

D

A

A

R

E

E

L

L

I

S

A

K

G

-
x
N

-

L

Q

E

G

A

H
x
N

E

K

V

I

F

F

F

S

A

D

L

F

E

E

S

D

L

L

S

N

I

V

P

P

V

T

V

V

A

A

A

A

I

I

N

N

G

G

A

I

C

A

L

L

G

G

G
|
G

G

G

L

L

E
|
E

L

M

A

C

L

L

A

A

C

A

H

D

Q

F

R

R

V

V

C

M

T

A

D

D

S

S

N

A

K

K

T

-

M

-

L

I

G

G

L

L

P
|
P

E
|
E

V

V

Q

K

L

L

G

G

L

I

L

Y

P
|
P

G
|
G

G

F

G

G

G

G

T

T

Q

V

R

R

L

L

P

P

R

R

L

L

V

I

G

G

I

V

A

D

K

N

S

A

L

V

D

E

M

W

M

I

L

A

T

S

G

G

K

K

Q

E

L

N

R

R

A

A

K

E

Q

D

A

A

L

L

K

K

M

V

G

S

L

A

V

V

D

D

D

A

V

V

V

V

P

T

E

A

S

D

I

K

L

L

L

G

Q

A

T

A

A

A

I

L

E

D

M

L

-

I

-

K

-

R

A

A

L

I

A

S

G

G

A

E

R

L

P

D

A

Y

K

K

K

A

P

K

K

R

L

Q

S

P

T

K

V

L

E

E

K

K

L

L

L

-

E

K

G

L

T

N

P

A

V

I

G

E

R

Q

N

M

I

M

I

A

F

F

E

E

Q

T

A

A

L

K

K

G

Q

F

V

V

N

A

K

G

K

Q

T

A

Q

G

G

P

N

N

Y

Y

P

P

A

A

P

P

E

V

K

E

I

A

I

I

D

K

C

T

V

I

R

Q

Q

K

G

A

V

A

T

N

K

F

G

G

I

R

V

D

K

K

G

A

L

L

E

E

V

V

E

E

A

A

Q

A

H

G

F

F

G

A

D

K

L

L

V

A

V

K

S

T

N

S

E

A

S

S

E

N

A

C

L

L

R

I

S

G

I

L

F

F

F

L

A

N

T

D

T

Q

E

E

M

L

K

K

K

K

E

K

S

A

G

K

A

V

G

Y

D

D

V

K

S

I

P

A

K

K

A

D

V

V

N

K

K

Q

V

A

M

A

V

V

L

L

G
|
G

G
x
A

G

G

L
x
I

M
|
M

G

G

G

G

G

G

I

I

A

A

S

Y

V

Q

T

S

T

A

T

S

K

K

A

G

K

-

I

T

P

P

V

I

R

L

V

M

K

K

D
|
D

I
|
I

S

N

E

E

I

H

G

G

L

I

S

E

N

Q

A

G

L

L

A

A

Y

E

A

A

Y

A

K

K

L

L

L

L

A

V

K

G

G

R

V

V

K

D

R

K

R

G

H

R

M

M

T

T

P

P

A

A

V

K

R

M

D

A

N

E

L

V

M

L

A

N

L

G

M

I

T

R

T

P

T

T

T

L

E

S

Y

Y

K

G

G

D

I

F

K

G

D

N

A

V

D

D

M

L

V

V

V

V

E

E

A
|
A

V
|
V

F
x
V

E
|
E

D

N

L

P

N

K

L

V

K
|
K

H

Q

Q

A

M
x
V

V

L

K

A

D

E

V

V

E

E

R

N

E

H

C

V

G

R

E

E

H

D

T

A

I

I

F

L

A

A

S

S

N
|
N

T
|
T

S
|
S

S

T

L

I

P

S

I

I

K

S

Q

L

I

L

A

A

E

K

A

A

A

L

E

K

R

R

P

P

E

E

N

N

V

F

I

V

G

G

L

M

H
|
H

Y

F

F

F

S

N

P

P

V

V

E

H

K

M

M

M

P

P

L

L

V

V

E
|
E

V

V

I

I

A

R

H

G

D

E

K

K

T

S

S

S

P

D

Q

L

T

A

I

V

A

A

T

T

T

T

V

V

A

A

F

Y

A

A

R

K

K

K

Q

M

G

G

K

K

T

N

P

P

I

I

V

V

V

V

K

N

D

D

G

C

A

P

G

G

F

F

Y

L

V

V

N
|
N

R

R

I

V

L

L

A

F

L

P

Y

Y

M

F

N

G

E

G

A

F

A

A

N

K

L

L

L

V

L

S

E

A

G

G

Q

V

S

D

V

F

D

V

H

R

L

I

D

D

K

K

A

V

L

M

V

E

K

K

F

F

G

G

F

W

P

P

V

M

G

G

P

P

M

A

T

Y

L

L

L

M

D

D

E

V

V

V

G

G

I

I

D

D

V

T

G

G

A

H

K

H

I

G

S

R

P

D

I

V

L

M

E

A

K

E

E

G

L

F

G

P

E

D

R

R

F

M

K

K

A

D

P

D

-

R

-

R

A

S

A

A

F

I

D

D

K

A

L

L

L

Y

A

E

D

A

D

K

R

R

K

L

G

G

R

Q

K

K

N

N

G

G

K

K

G

G

F

F

Y

Y

Q

A

Y

Y

G

E

A

A

K

D

S

K

K

K

-

G

-

K

-

Q

K

K

K

K

L

L

V

V

D

D

E

S

S

S

V

V

Y

L

G

E

V

V

L

L

G

-

L

-

T

-

P

-

G

-

A

-

D

-

G

-

E

K

P

P

I

I

A

V

L

Y

A

E

E

Q

R

R

C

D

V

V

-

T

-

D

-

E

-

D

-

I

-

I

-

N

-

W

-

M

-

M

V

I

Q

P

M

L

L

C

N

L

E

E

A

T

V

V

R

R

C

C

L

L

E

E

E

D

G

G

I

I

I

V

A

E

S

T

P

A

R

A

D

E

G

A

D

D

I

M

G

G

A

L

I

V

F

Y

G

G

I

I

G

G

F

F

P

P

P

L

F

F

L

R

G

G

G

G

P

A

F

L

R

R

Y

Y

M

I

D

D

S

S

L

I

G

G

A

V

K

A

H

E

L

F

V

V

E

A

T

L

L

A

K

D

R

Q

Y

Y

Q

A

D

-

Q

E

F

L

G

G

D

A

R

L

F

Y

A

H

P

P

C

T

D

A

R

K

L

L

V

R

E

E

M

M

A

A

E

K

S

N

G

G

S

Q

K

S

F

F

Y

F

active site: A69 (= A67), N89 (vs. gap), N93 (≠ H89), G117 (= G113), E120 (= E116), P139 (= P137), E140 (= E138), P147 (= P145), G148 (= G146), S430 (= S427), H451 (= H448), E463 (= E460), N501 (= N498)
binding nicotinamide-adenine-dinucleotide: G321 (= G317), A322 (≠ G318), I324 (≠ L320), M325 (= M321), D344 (= D341), I345 (= I342), A400 (= A397), V401 (= V398), V402 (≠ F399), E403 (= E400), K408 (= K405), V411 (≠ M408), N428 (= N425), T429 (= T426), S430 (= S427)

P28793 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Pseudomonas fragi (see paper)
40% identity, 100% coverage: 3:705/706 of query aligns to 6:714/715 of P28793

query
sites
P28793

K

K

S

A

F

I

T

T

L

V

N

T

R

A

L

L

D

E

N

S

G

G

I

I

A

V

V

E

L

L

T

K

M

F

D

D

V

L

P

K

G

G

E

E

T

S

M

V

N

N

T

K

L

F

R

N

A

R

E

L

F

T

G

L

P

N

E

E

I

L

C

R

E

Q

M

A

L

V

A

D

E

A

I

I

K

K

A

A

D

D

A

A

G

S

I

V

R

K

G

G

V

V

V

I

I

V

I

S

S

S

G

G

K

K

K

-

D

D

S

V

F

F

V

I

A

V

G

G

A

A

D

D

I

I

S

T

M

E

L

F

D

V

A

E

C

N

A

F

T

K

A

L

E

P

D

D

A

A

R

E

E

L

L

I

S

A

K

G

-

N

-

L

Q

E

G

A

H

N

E

K

V

I

F

F

F

S

A

D

L

F

E

E

S

D

L

L

S

N

I

V

P

P

V

T

V

V

A

A

A

A

I

I

N

N

G

G

A

I

C

A

L

L

G

G

G

G

G

G

L

L

E

E

L

M

A

C

L

L

A

A

C

A

H

D

Q

F

R

R

V

V

C

M

T

A

D

D

S

S

N

A

K

K

T

-

M

-

L

I

G

G

L

L

P

P

E

E

V

V

Q

K

L

L

G

G

L

I

L

Y

P

P

G

G

G

F

G

G

G

G

T

T

Q

V

R

R

L

L

P

P

R

R

L

L

V

I

G

G

I

V

A

D

K

N

S

A

L

V

D

E

M

W

M

I

L

A

T

S

G

G

K

K

Q

E

L

N

R

R

A

A

K

E

Q

D

A

A

L

L

K

K

M

V

G

S

L

A

V

V

D

D

D

A

V

V

V

V

P

T

E

A

S

D

I

K

L

L

L

G

Q

A

T

A

A

A

I

L

E

D

M

L

-

I

-

K

-

R

A

A

L

I

A

S

G

G

A

E

R

L

P

D

A

Y

K

K

K

A

P

K

K

R

L

Q

S

P

T

K

V

L

E

E

K

K

L

L

L

-

E

K

G

L

T

N

P

A

V

I

G

E

R

Q

N

M

I

M

I

A

F

F

E

E

Q

T

A

A

L

K

K

G

Q

F

V

V

N

A

K

G

K

Q

T

A

Q

G

G

P

N

N

Y

Y

P

P

A

A

P

P

E

V

K

E

I

A

I

I

D

K

C

T

V

I

R

Q

Q

K

G

A

V

A

T

N

K

F

G

G

I

R

V

D

K

K

G

A

L

L

E

E

V

V

E

E

A

A

Q

A

H

G

F

F

G

A

D

K

L

L

V

A

V

K

S

T

N

S

E

A

S

S

E

N

A

C

L

L

R

I

S

G

I

L

F

F

F

L

A

N

T
x
D

T

Q

E

E

M

L

K

K

K

K

E

K

S

A

G

K

A

V

G

Y

D

D

V

K

S

I

P

A

K

K

A

D

V

V

N

K

K

Q

V

A

M

A

V

V

L

L

G

G

G

A

G

G

L

I

M
|
M

G

G

G

G

G

G

I

I

A

A

S

Y

V

Q

T

S

T

A

T

S

K

K

A

G

K

-

I

T

P

P

V

I

R

L

V

M

K

K

D
|
D

I

I

S

N

E

E

I

H

G

G

L

I

S

E

N

Q

A

G

L

L

A

A

Y

E

A

A

Y

A

K

K

L

L

L

L

A

V

K

G

G

R

V

V

K

D

R

K

R

G

H

R

M

M

T

T

P

P

A

A

V

K

R

M

D

A

N

E

L

V

M

L

A

N

L

G

M

I

T

R

T

P

T

T

T

L

E

S

Y

Y

K

G

G

D

I

F

K

G

D

N

A

V

D

D

M

L

V

V

V

V

E

E

A

A

V
|
V

F
x
V

E
|
E

D

N

L

P

N

K

L

V

K
|
K

H

Q

Q

A

M

V

V

L

K

A

D

E

V

V

E

E

R

N

E

H

C

V

G

R

E

E

H

D

T

A

I

I

F

L

A

A

S

S

N

N

T

T

S
|
S

S

T

L

I

P

S

I

I

K

S

Q

L

I

L

A

A

E

K

A

A

A

L

E

K

R

R

P

P

E

E

N

N

V

F

I

V

G

G

L

M

H

H

Y

F

F

F

S
x
N

P

P

V

V

E

H

K

M

M

M

P

P

L

L

V

V

E

E

V

V

I

I

A

R

H

G

D

E

K

K

T

S

S

S

P

D

Q

L

T

A

I

V

A

A

T

T

T

T

V

V

A

A

F

Y

A

A

R

K

K

K

Q

M

G

G

K

K

T

N

P

P

I

I

V

V

V

V

K

N

D

D

G

C

A

P

G

G

F

F

Y

L

V

V

N
|
N

R

R

I

V

L

L

A

F

L

P

Y

Y

M

F

N

G

E

G

A

F

A

A

N

K

L

L

L

V

L

S

E

A

G

G

Q

V

S

D

V

F

D

V

H

R

L

I

D

D

K

K

A

V

L

M

V

E

K

K

F

F

G

G

F

W

P

P

V

M

G

G

P

P

M

A

T

Y

L

L

L

M

D

D

E

V

V

V

G

G

I

I

D

D

V

T

G

G

A

H

K

H

I

G

S

R

P

D

I

V

L

M

E

A

K

E

E

G

L

F

G

P

E

D

R

R

F

M

K

K

A

D

P

D

-

R

-

R

A

S

A

A

F

I

D

D

K

A

L

L

L

Y

A

E

D

A

D

K

R

R

K

L

G

G

R

Q

K

K

N

N

G

G

K

K

G

G

F

F

Y

Y

Q

A

Y

Y

G

E

A

A

K

D

S

K

K

K

-

G

-

K

-

Q

K

K

K

K

L

L

V

V

D

D

E

S

S

S

V

V

Y

L

G

E

V

V

L

L

G

-

L

-

T

-

P

-

G

-

A

-

D

-

G

-

E

K

P

P

I

I

A

V

L

Y

A

E

E

Q

R

R

C

D

V

V

-

T

-

D

-

E

-

D

-

I

-

I

-

N

-

W

-

M

-

M

V

I

Q

P

M

L

L

C

N

L

E

E

A

T

V

V

R

R

C

C

L

L

E

E

E

D

G

G

I

I

I

V

A

E

S

T

P

A

R

A

D

E

G

A

D

D

I

M

G

G

A

L

I

V

F
x
Y

G

G

I

I

G

G

F

F

P

P

P

L

F

F

L

R

G

G

G

G

P

A

F

L

R

R

Y

Y

M

I

D

D

S

S

L

I

G

G

A

V

K

A

H

E

L

F

V

V

E

A

T

L

L

A

K

D

R

Q

Y

Y

Q

A

D

-

Q

E

F

L

G

G

D

A

R

L

F

Y

A

H

P

P

C

T

D

A

R

K

L

L

V

R

E

E

M

M

A

A

E

K

S

N

G

G

S

Q

K

S

F

F

Y

F

D297 (≠ T293) binding
M325 (= M321) binding
D344 (= D341) binding
VVE 401:403 (≠ VFE 398:400) binding
K408 (= K405) binding
S430 (= S427) binding
N454 (≠ S451) binding
N501 (= N498) binding
Y660 (≠ F650) binding

1wdmA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form i (native3) (see paper)
40% identity, 100% coverage: 3:705/706 of query aligns to 6:706/707 of 1wdmA

query
sites
1wdmA

K

K

S

A

F

I

T

T

L

V

N

T

R

A

L

L

D

E

N

S

G

G

I

I

A

V

V

E

L

L

T

K

M

F

D

D

V

L

P

K

G

G

E

E

T

S

M

V

N

N

T

K

L

F

R

N

A

R

E

L

F

T

G

L

P

N

E

E

I

L

C

R

E

Q

M

A

L

V

A

D

E

A

I

I

K

K

A

A

D

D

A

A

G

S

I

V

R

K

G

G

V

V

V

I

I

V

I

S

S

S

G

G

K

K

K

-

D

D

S

V

F

F

V

I

A

V

G

G

A
|
A

D

D

I

I

S

T

M

E

L

F

D

V

A

E

C

N

A

F

T

K

A

L

E

P

D

D

A

A

R

E

E

L

L

I

S

A

K

G

-
x
N

-

L

Q

E

G

A

H
x
N

E

K

V

I

F

F

F

S

A

D

L

F

E

E

S

D

L

L

S

N

I

V

P

P

V

T

V

V

A

A

A

A

I

I

N

N

G

G

A

I

C

A

L

L

G

G

G
|
G

G

G

L

L

E
|
E

L

M

A

C

L

L

A

A

C

A

H

D

Q

F

R

R

V

V

C

M

T

A

D

D

S

S

N

A

K

K

T

-

M

-

L

I

G

G

L

L

P
|
P

E
|
E

V

V

Q
x
K

L

L

G

G

L

I

L

Y

P
|
P

G
|
G

G

F

G

G

G

G

T

T

Q

V

R

R

L

L

P

P

R

R

L

L

V

I

G

G

I

V

A

D

K

N

S

A

L

V

D

E

M

W

M

I

L

A

T

S

G

G

K

K

Q

E

L

N

R

R

A

A

K

E

Q

D

A

A

L

L

K

K

M

V

G

S

L

A

V

V

D

D

D

A

V

V

V

V

P

T

E

A

S

D

I

K

L

L

L

G

Q

A

T

A

A

A

I

L

E

D

M

L

-

I

-

K

-

R

A

A

L

I

A

S

G

G

A

E

R

L

P

D

A

Y

K

K

K

A

P

K

K

R

L

Q

S

P

T

K

V

L

E

E

K

K

L

L

L

-

E

K

G

L

T

N

P

A

V

I

G

E

R

Q

N

M

I

M

I

A

F

F

E

E

Q

T

A

A

L

K

K

G

Q

F

V

V

N

A

K

G

K

Q

T

A

Q

G

G

P

N

N

Y

Y

P

P

A

A

P

P

E

V

K

E

I

A

I

I

D

K

C

T

V

I

R

Q

Q

K

G

A

V

A

T

N

K

F

G

G

I

R

V

D

K

K

G

A

L

L

E

E

V

V

E

E

A

A

Q

A

H

G

F

F

G

A

D

K

L

L

V

A

V

K

S

T

N

S

E

A

S

S

E

N

A

C

L

L

R

I

S

G

I

L

F

F

F

L

A

N

T
x
D

T

Q

E

E

M

L

K

K

K

K

E

K

S

A

G

K

A

V

G

Y

D

D

V

K

S

I

P

A

K

K

A

D

V

V

N

K

K

Q

V

A

M

A

V

V

L

L

G
|
G

G
x
A

G

G

L
x
I

M
|
M

G

G

G

G

G

G

I

I

A

A

S

Y

V

Q

T

S

T

A

T

S

K

K

A

G

K

-

I

T

P

P

V

I

R

L

V

M

K
|
K

D
|
D

I
|
I

S

N

E

E

I

H

G

G

L

I

S

E

N

Q

A

G

L

L

A

A

Y

E

A

A

Y

A

K

K

L

L

L

L

A

V

K

G

G

R

V

V

K

D

R

K

R

G

H

R

M

M

T

T

P

P

A

A

V

K

R

M

D

A

N

E

L

V

M

L

A

N

L

G

M

I

T

R

T

P

T

T

T

L

E

S

Y

Y

K

G

G

D

I

F

K

G

D

N

A

V

D

D

M

L

V

V

V

V

E

E

A
|
A

V
|
V

F

V

E
|
E

D

N

L

P

N

K

L

V

K
|
K

H

Q

Q

A

M

V

V

L

K

A

D

E

V

V

E

E

R

N

E

H

C

V

G

R

E

E

H

D

T

A

I

I

F

L

A

A

S

S

N
|
N

T
|
T

S
|
S

S

T

L

I

P

S

I

I

K

S

Q

L

I

L

A

A

E

K

A

A

A

L

E

K

R

R

P

P

E

E

N

N

V

F

I

V

G

G

L

M

H
|
H

Y
x
F

F

F

S
x
N

P

P

V

V

E

H

K

M

M
|
M

P

P

L

L

V

V

E
|
E

V

V

I

I

A

R

H

G

D

E

K

K

T

S

S

S

P

D

Q

L

T

A

I

V

A

A

T

T

T

T

V

V

A

A

F

Y

A

A

R

K

K

K

Q

M

G

G

K

K

T

N

P

P

I

I

V

V

V

V

K

N

D

D

G

C

A

P

G

G

F

F

Y

L

V

V

N
|
N

R

R

I

V

L

L

A

F

L

P

Y

Y

M

F

N

G

E

G

A

F

A

A

N

K

L

L

L

V

L

S

E

A

G

G

Q

V

S

D

V

F

D

V

H

R

L

I

D

D

K

K

A

V

L

M

V

E

K

K

F

F

G

G

F

W

P
|
P

V
x
M

G

G

P

P

M

A

T

Y

L

L

L

M

D

D

E

V

V
|
V

G

G

I

I

D

D

V

T

G

G

A

H

K

H

I

G

S

R

P

D

I

V

L

M

E

A

K

E

E

G

L

F

G

P

E

D

R

R

F

M

K

K

A

D

P

D

-

R

-

R

A

S

A

A

F

I

D

D

K

A

L

L

L

Y

A

E

D

A

D

K

R

R

K

L

G

G

R

Q

K

K

N

N

G

G

K

K

G

G

F

F

Y

Y

Q

A

Y

Y

G

-

A

-

K

-

S

-

K

-

K

K

K

K

L

L

V

V

D

D

E

S

S

S

V

V

Y

L

G

E

V

V

L

L

G

-

L

-

T

-

P

-

G

-

A

-

D

-

G

-

E

K

P

P

I

I

A

V

L

Y

A

E

E

Q

R

R

C

D

V

V

-

T

-

D

-

E

-

D

-

I

-

I

-

N

-

W

-

M

-

M

V

I

Q

P

M

L

L

C

N

L

E

E

A

T

V

V

R

R

C

C

L

L

E

E

E

D

G

G

I

I

I

V

A

E

S

T

P

A

R

A

D

E

G

A

D

D

I

M

G

G

A

L

I
x
V

F
x
Y

G

G

I

I

G
|
G

F

F

P

P

P
x
L

F

F

L

R

G

G

G

G

P

A

F

L

R

R

Y

Y

M

I

D

D

S

S

L

I

G

G

A

V

K

A

H

E

L

F

V

V

E

A

T

L

L

A

K

D

R

Q

Y

Y

Q

A

D

-

Q

E

F

L

G

G

D

A

R

L

F

Y

A

H

P

P

C

T

D

A

R

K

L

L

V

R

E

E

M

M

A

A

E

K

S

N

G

G

S

Q

K

S

F

F

Y

F

active site: A69 (= A67), N89 (vs. gap), N93 (≠ H89), G117 (= G113), E120 (= E116), P139 (= P137), E140 (= E138), P147 (= P145), G148 (= G146), S430 (= S427), H451 (= H448), E463 (= E460), N501 (= N498)
binding acetyl coenzyme *a: K142 (≠ Q140), D297 (≠ T293), M459 (= M456), N501 (= N498), P534 (= P531), M535 (≠ V532), V544 (= V541), V651 (≠ I649), Y652 (≠ F650), G655 (= G653), L658 (≠ P656)
binding nicotinamide-adenine-dinucleotide: G321 (= G317), A322 (≠ G318), I324 (≠ L320), M325 (= M321), K343 (= K340), D344 (= D341), I345 (= I342), A400 (= A397), V401 (= V398), E403 (= E400), K408 (= K405), N428 (= N425), T429 (= T426), S430 (= S427), F452 (≠ Y449), N454 (≠ S451)

P21177 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Escherichia coli (strain K12) (see 2 papers)
36% identity, 99% coverage: 4:705/706 of query aligns to 7:714/729 of P21177

query
sites
P21177

S

T

F

L

T

Y

L

L

N

D

R

W

L

L

D

E

N

D

G

G

I

I

A

A

V

E

L

L

T

V

M

F

D

D

V

A

P

P

G

G

E

-

T

S

M

V

N

N

T

K

L

L

R

D

A

T

E

A

F

T

G

V

P

A

E

S

I

L

C

G

E

E

M

A

L

I

A

G

E

V

I

L

K

E

A

Q

D

Q

A

S

G

D

I

L

R

K

G

G

V

L

V

L

I

L

I

R

S

S

G

N

K

K

K

A

D

-

S

A

F

F

V

I

A

V

G

G

A

A

D

D

I

I

S

T

-

E

M

F

L

L

D

S

A

L

C

F

A

L

T

V

A

P

E

E

D

E

A

-

R

-

E

Q

L

L

S

S

K

Q

Q

W

G

L

H

H

-

F

-

A

-

N

E

S

V

V

F

F

F

N

A

R

L

L

E

E

S

D

L

L

S

P

I

V

P

P

V

T

V

I

A

A

A

A

I

V

N

N

G

G

A

Y

C

A

L

L

G

G

G
|
G

G

G

L

C

E

E

L

C

A

V

L

L

A

A

C

T

H

D

Q

Y

R

R

V

L

C

A

T

T

D

P

S

D

N

L

K

R

T

-

M

-

L

I

G

G

L

L

P

P

E

E

V

T

Q

K

L

L

G

G

L

I

L

M

P

P

G

G

G

F

G

G

G

G

T

S

Q

V

R

R

L

M

P

P

R

R

L

M

V

L

G

G

I

A

A

D

K

S

S

A

L

L

D

E

M

I

M

I

L

A

T

A

G

G

K

K

Q

D

L

V

R

G

A

A

K

D

Q

Q

A

A

L

L

K

K

M

I

G

G

L

L

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G116 (= G113) mutation to F: Absence of both enoyl-CoA hydratase and 3-hydroxyacyl-CoA epimerase activities. Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase is only slightly affected.
G322 (= G319) mutation to A: 10-fold increase in KM for NADH.
H450 (= H448) active site, For 3-hydroxyacyl-CoA dehydrogenase activity; mutation H->A,Q: Almost complete loss of 3-hydroxyacyl-CoA dehydrogenase activity.

6tnmA E. Coli aerobic trifunctional enzyme subunit-alpha (see paper)
36% identity, 99% coverage: 4:705/706 of query aligns to 7:714/719 of 6tnmA

query
sites
6tnmA

S

T

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