SitesBLAST

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
44% identity, 45% coverage: 115:208/209 of query aligns to 96:188/203 of P07464

query
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P07464

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H115 (= H134) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ A162) binding in other chain
A160 (≠ M180) binding in other chain
TK 165:166 (= TK 185:186) binding acetyl-CoA
R180 (= R200) binding acetyl-CoA
R183 (≠ G203) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding substrate
71 binding substrate
85 binding in other chain; binding in other chain
93 binding substrate

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
44% identity, 45% coverage: 115:208/209 of query aligns to 95:187/201 of 1krvA

query
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1krvA

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binding 4-nitrophenyl beta-D-galactopyranoside: M126 (≠ S147)
binding coenzyme a: A104 (= A124), S110 (≠ Y130), T112 (≠ F132), W138 (= W159), G140 (= G161), S141 (≠ A162), N146 (≠ V167), A159 (≠ M180), P177 (= P198), R179 (= R200), R182 (≠ G203)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16, 25, 70, 82, 84, 90, 92

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
44% identity, 45% coverage: 115:208/209 of query aligns to 95:187/201 of 1kruA

query
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1kruA

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binding coenzyme a: H114 (= H134), W138 (= W159), G140 (= G161), S141 (≠ A162), G158 (= G179), A159 (≠ M180), P177 (= P198), R182 (≠ G203)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: L102 (≠ R122), H114 (= H134), M126 (≠ S147)

Sites not aligning to the query:

binding 1-methylethyl 1-thio-beta-D-galactopyranoside: 16, 22, 25, 62, 70, 82, 82, 84, 90, 92

4mzuF Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
36% identity, 61% coverage: 77:203/209 of query aligns to 30:142/294 of 4mzuF

query
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4mzuF

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binding coenzyme a: N81 (≠ H134), D82 (≠ G135), K83 (≠ M136), G100 (= G161), A101 (= A162), L106 (≠ V167), G118 (= G179), A119 (≠ M180), T124 (= T185), K125 (= K186), I134 (≠ A195), N136 (= N197), R139 (= R200)
binding thymine: C38 (≠ A85)

Sites not aligning to the query:

4mzuB Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
36% identity, 61% coverage: 77:203/209 of query aligns to 30:141/290 of 4mzuB

query
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4mzuB

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binding coenzyme a: F72 (≠ R122), T80 (≠ N133), N81 (≠ H134), D82 (≠ G135), K83 (≠ M136), P85 (= P138), G99 (= G161), A100 (= A162), G117 (= G179), A118 (≠ M180), T123 (= T185), K124 (= K186), I133 (≠ A195), R138 (= R200)
binding magnesium ion: G58 (= G105), N76 (= N126)
binding thymine: C38 (≠ A85), A39 (= A86)

Sites not aligning to the query:

binding coenzyme a: 199
binding thymine: 20, 21
binding thymidine-5'-diphosphate: 171, 176, 189, 191, 202, 203, 270

8j40A Crystal structure of catb8 in complex with chloramphenicol (see paper)
37% identity, 52% coverage: 98:205/209 of query aligns to 54:163/209 of 8j40A

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8j40A

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binding chloramphenicol: Y90 (≠ H134), W117 (= W159), V135 (= V177), S138 (≠ M180)

Sites not aligning to the query:

binding chloramphenicol: 7, 8, 9, 29, 31, 47, 50, 187, 190, 191, 202, 203, 206

5u2kA Crystal structure of galactoside o-acetyltransferase complex with coa (h3 space group)
27% identity, 83% coverage: 29:202/209 of query aligns to 1:181/190 of 5u2kA

query
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5u2kA

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binding coenzyme a: M90 (≠ D109), Y110 (= Y130), A112 (≠ F132), H114 (= H134), L146 (≠ V167), T164 (= T185), K165 (= K186)

2xatA Complex of the hexapeptide xenobiotic acetyltransferase with chloramphenicol and desulfo-coenzyme a (see paper)
37% identity, 52% coverage: 98:205/209 of query aligns to 53:162/208 of 2xatA

query
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2xatA

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binding desulfo-coenzyme a: G118 (= G161), T119 (≠ A162), G136 (= G179), S137 (≠ M180), G152 (≠ A195), G153 (= G196), I159 (= I202)

Sites not aligning to the query:

binding chloramphenicol: 6, 7, 8, 28

4isxA The crystal structure of maltose o-acetyltransferase from clostridium difficile 630 in complex with acetyl-coa
44% identity, 43% coverage: 114:202/209 of query aligns to 94:181/186 of 4isxA

query
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4isxA

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I

binding acetyl coenzyme *a: A104 (= A124), W138 (= W159), G140 (= G161), G141 (≠ A162), A159 (≠ M180), V174 (≠ A195)

Sites not aligning to the query:

binding acetyl coenzyme *a: 84, 182

4hurA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with acetyl coenzyme a (see paper)
35% identity, 57% coverage: 87:205/209 of query aligns to 47:166/211 of 4hurA

query
sites
4hurA

D

D

S

Q

F

V

L

L

H
x
Y

G

H

P

Y

I

E

V

V

M

I

G

G

N

D

E

K

V

L

A

I

I

I

N

G

H

R

G

F

C

C

S
|
S

L
x
I

D
x
G

G

P

G

G

R

T

G

T

K

F

I

I

V

M

I

N

G

G

D
x
A

Q
x
N

T

H

R

R

I

M

-

D

A

G

N

S

N

T

V

Y

T

P

I

F

Y

H

A

L

F

F

N

R

H

M

G

G

M

W

H

E

P

K

D

Y

T

M

P

P

I

S

Y

L

Q

K

Q

D

S

L

S

P

Q

L

S
x
K

K

G

G

D

I

I

V

E

I

I

G

G

R

N

D

D

V

V

W
|
W

I
|
I

G
|
G

A
x
R

Q

D

A

V

G

T

I

I

V
x
M

D

P

G

G

V

V

T

K

I

I

G

G

D

D

C

G

A

A

V

I

I

I

G
x
A

M
x
A

G

E

C

A

I

V

V

V

T
|
T

K

K

D

N

V

V

P

A

D

P

Y

Y

A

S

I

I

V

V

A
x
G

G

G

N

N

P
|
P

A

L

R

K

I

F

I
|
I

G
x
R

D

K

R

R

binding acetyl coenzyme *a: Y51 (≠ H91), S67 (= S107), I68 (≠ L108), G69 (≠ D109), A79 (≠ D119), N80 (≠ Q120), K110 (≠ S149), W120 (= W159), I121 (= I160), G122 (= G161), R123 (≠ A162), M128 (≠ V167), A140 (≠ G179), A141 (≠ M180), T146 (= T185), G156 (≠ A195), P159 (= P198), I163 (= I202), R164 (≠ G203)

4husA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with virginiamycin m1 (see paper)
35% identity, 57% coverage: 87:205/209 of query aligns to 47:166/212 of 4husA

query
sites
4husA

D

D

S

Q

F

V

L

L

H

Y

G

H

P

Y

I

E

V

V

M

I

G

G

N

D

E

K

V

L

A

I

I

I

N

G

H

R

G

F

C

C

S

S

L

I

D

G

G

P

G

G

R

T

G

T

K

F

I

I

V

M

I

N

G

G

D

A

Q

N

T

H

R

R

I

M

-

D

A

G

N

S

N

T

V

Y

T

P

I

F

Y

H

A

L

F

F

N

R

H

M

G

G

M

W

H

E

P

K

D

Y

T

M

P

P

I

S

Y

L

Q

K

Q

D

S

L

S

P

Q

L

S

K

K

G

G

D

I

I

V

E

I

I

G

G

R

N

D

D

V

V

W

W

I

I

G

G

A

R

Q

D

A

V

G

T

I

I

V

M

D

P

G

G

V

V

T

K

I

I

G

G

D

D

C

G

A

A

V

I

I

I

G

A

M

A

G

E

C

A

I

V

V

V

T

T

K

K

D

N

V

V

P

A

D

P

Y

Y

A

S

I

I

V

V

A

G

G

G

N

N

P

P

A

L

R

K

I

F

I

I

G

R

D

K

R

R

Sites not aligning to the query:

binding virginiamycin m1: 17, 36, 38

6x3jA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f0224 (46) (see paper)
33% identity, 63% coverage: 74:205/209 of query aligns to 27:166/206 of 6x3jA

query
sites
6x3jA

R

E

D

N

I

I

L

L

I

V

G

G

D

E

-

Y

-

S

-

Y

-

D

-

S

-

K

R

R

C

G

M

E

I

S

A

F

A

E

D

D

S

Q

F

V

L

L

H
x
Y

G

H

P
x
Y

I

E

V
|
V

M

I

G

G

N

D

E

K

V

L

A

I

I

I

N

G

H

R

G

F

C

C

S

S

L

I

D
x
G

G

P

G

G

R

T

G

T

K

F

I

I

V

M

I

N

G

G

D
x
A

Q

N

T
x
H

R

R

I

M

-

D

A

G

N

S

N

T

V

Y

T

P

I

F

Y
x
H

A
x
L

F

F

N

R

H

M

G

G

M

W

H

E

P

K

D

Y

T
x
M

P
|
P

I

S

Y

L

Q

K

Q

D

S
x
L

S

P

Q

L

S

K

K

G

G

D

I

I

V

E

I

I

G

G

R

N

D

D

V

V

W
|
W

I

I

G
|
G

A

R

Q

D

A

V

G

T

I

I

V

M

D

P

G

G

V

V

T

K

I

I

G

G

D

D

C

G

A

A

V

I

I

I

G

A

M

A

G

E

C

A

I

V

V

V

T

T

K

K

D

N

V

V

P

A

D

P

Y

Y

A

S

I

I

V

V

A

G

G

G

N

N

P

P

A

L

R

K

I

F

I

I

G

R

D

K

R

R

binding (2R)-2-[(3S,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-4,12-dimethyl-1,7,22-trioxo-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-3-yl]propyl isoquinolin-3-ylcarbamate: Y51 (≠ H91), Y53 (≠ P93), V55 (= V95), H91 (≠ Y130), L92 (≠ A131), M101 (≠ T140), P102 (= P141), L107 (≠ S146)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (≠ D109), A79 (≠ D119), H81 (≠ T121), W120 (= W159), G122 (= G161)

6x3cA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
35% identity, 57% coverage: 87:205/209 of query aligns to 47:166/207 of 6x3cA

query
sites
6x3cA

D

D

S

Q

F

V

L

L

H
x
Y

G

H

P
x
Y

I

E

V

V

M

I

G

G

N

D

E

K

V

L

A

I

I

I

N

G

H

R

G

F

C

C

S

S

L

I

D
x
G

G

P

G

G

R

T

G

T

K

F

I

I

V

M

I

N

G
|
G

D

A

Q
x
N

T
x
H

R

R

I

M

-

D

A

G

N

S

N

T

V

Y

T

P

I

F

Y
x
H

A
x
L

F

F

N

R

H

M

G

G

M

W

H

E

P

K

D

Y

T
x
M

P
|
P

I

S

Y
x
L

Q

K

Q

D

S

L

S

P

Q

L

S

K

K

G

G

D

I

I

V

E

I

I

G

G

R

N

D

D

V

V

W
|
W

I

I

G
|
G

A
x
R

Q

D

A

V

G

T

I

I

V
x
M

D

P

G

G

V

V

T

K

I

I

G

G

D

D

C

G

A

A

V

I

I

I

G
x
A

M
x
A

G

E

C

A

I

V

V

V

T

T

K

K

D

N

V

V

P

A

D

P

Y

Y

A

S

I

I

V

V

A

G

G

G

N

N

P

P

A

L

R

K

I

F

I

I

G

R

D

K

R

R

binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: Y53 (≠ P93), G78 (= G118), N80 (≠ Q120), H81 (≠ T121), H91 (≠ Y130), L92 (≠ A131), M101 (≠ T140), P102 (= P141), L104 (≠ Y143)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: Y51 (≠ H91), G69 (≠ D109), N80 (≠ Q120), H81 (≠ T121), W120 (= W159), G122 (= G161), R123 (≠ A162), M128 (≠ V167), A140 (≠ G179), A141 (≠ M180)

Sites not aligning to the query:

binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: 17, 36

6x3cE Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
33% identity, 63% coverage: 74:205/209 of query aligns to 27:166/203 of 6x3cE

query
sites
6x3cE

R

E

D

N

I

I

L

L

I

V

G

G

D

E

-

Y

-

S

-

Y

-

D

-

S

-

K

R

R

C

G

M

E

I

S

A

F

A

E

D

D

S

Q

F

V

L

L

H
x
Y

G

H

P
x
Y

I

E

V

V

M

I

G

G

N

D

E

K

V

L

A

I

I

I

N

G

H

R

G

F

C

C

S

S

L

I

D
x
G

G

P

G

G

R

T

G

T

K

F

I

I

V

M

I

N

G
|
G

D

A

Q
x
N

T
x
H

R

R

I
x
M

-

D

A

G

N

S

N

T

V

Y

T

P

I

F

Y
x
H

A
x
L

F

F

N

R

H

M

G

G

M

W

H

E

P

K

D

Y

T
x
M

P
|
P

I

S

Y

L

Q

K

Q

D

S

L

S

P

Q

L

S

K

K

G

G

D

I

I

V

E

I

I

G

G

R

N

D

D

V

V

W
|
W

I

I

G
|
G

A

R

Q

D

A

V

G

T

I

I

V

M

D

P

G

G

V

V

T

K

I

I

G

G

D

D

C

G

A

A

V

I

I

I

G
x
A

M
x
A

G

E

C

A

I

V

V

V

T

T

K

K

D

N

V

V

P

A

D

P

Y

Y

A

S

I

I

V

V

A

G

G

G

N
|
N

P
|
P

A

L

R

K

I

F

I

I

G

R

D

K

R

R

binding magnesium ion: N158 (= N197), P159 (= P198)
binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: Y53 (≠ P93), G78 (= G118), N80 (≠ Q120), H81 (≠ T121), H91 (≠ Y130), L92 (≠ A131), M101 (≠ T140), P102 (= P141)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: Y51 (≠ H91), G69 (≠ D109), H81 (≠ T121), M83 (≠ I123), W120 (= W159), G122 (= G161), A140 (≠ G179), A141 (≠ M180)

A1ADJ6 Polysialic acid O-acetyltransferase; Capsule O-acetyl transferase; EC 2.3.1.136 from Escherichia coli O1:K1 / APEC (see paper)
35% identity, 53% coverage: 92:202/209 of query aligns to 167:277/307 of A1ADJ6

query
sites
A1ADJ6

G

S

P

K

I

V

V

I

M

I

G

G

N

R

E

R

V

T

A

T

I

I

N

G

H

A

G

G

C

F

S

E

L

V

D

V

G

T

G

D

R

K

G

C

K

N

I

V

V

T

I

I

G

G

D

H

Q

D

T

C

R

M

I

I

A

A

N

R

N

D

V

V

T

I

I

L

Y

R

A

A

F

S

N

D

H

G

G

-

M

-

H
|
H

P

P

D

I

T

F

P

D

I

I

Y

H

Q

S

Q

K

S

K

-

R

-

I

S

N

Q

W

S

A

K

K

G

D

I

I

V

I

I

I

G

S

R

S

D

Y

V

V

W
|
W

I

V

G

G

A

R

Q

N

A

V

G

S

I

I

V

M

D

K

G

G

V

V

T

S

I

V

G

G

D

S

C

G

A

S

V

V

I

I

G

G

M

Y

G

G

C

S

I

I

V

V

T

T

K

K

D

D

V

V

P

P

D

S

Y

M

A

C

I

A

V

A

A

A

G

G

N

N

P

P

A

A

R

K

I

I

H210 (= H137) mutation to A: Loss of activity.
W234 (= W159) mutation to A: Loss of activity.

G3XD01 UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronate N-acetyltransferase; UDP-D-GlcNAc3NA N-acetyltransferase; UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronic acid 3-N-acetyltransferase; EC 2.3.1.201 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
35% identity, 43% coverage: 114:203/209 of query aligns to 50:153/191 of G3XD01

query
sites
G3XD01

K

K

I

V

V

V

I

I

G

G

D

D

Q

R

T

C

R

K

I

I

A

Q

N

N

V

V

T

S

I

V

Y

Y

-

D

-

N

-

V

-

T

-

L

-

E

-

G

-

V

-

F

-

C

-

G

-

P

-

S

-

M

A

V

F

F

N

T

H

N

G

V

M

Y

H

N

P

P

D

R

T

S

P

L

I

I

Y

-

Q

E

Q

R

S

K

S

D

Q

Q

S

Y

K

R

G

N

I

T

V

L

I

V

G

K

R

K

D

G

V

A

W

T

I

L

G

G

A

A

Q

N

A

C

G

T

I

I

V

V

D

C

G

G

V

V

T

T

I

I

G

G

D

E

C

Y

A

A

V

F

I

V

G

G

M

A

G

G

C

A

I

V

V

I

T

N

K
|
K

D

N

V

V

P

P

D

S

Y

Y

A

A

I

L

V

M

A

V

G

G

N

V

P

P

A

A

R

R

I

Q

I

I

G

G

K136 (= K186) mutation to A: Reduction in catalytic activity.; mutation to R: No effect on catalytic activity.

3ectA Crystal structure of the hexapeptide-repeat containing- acetyltransferase vca0836 from vibrio cholerae
31% identity, 79% coverage: 43:207/209 of query aligns to 26:176/176 of 3ectA

query
sites
3ectA

E

E

V

I

Q

N

Q

Q

A

S

L

L

M

D

S

E

V

A

E

E

T

R

V

Y

S

A

I

L

G

Q

K

R

E

E

C

L

F

F

I

G

A

H

P

L

Q

G

A

H

K

K

L

S

F

C

A

V

E

Q

P

P

G

P

R

F

D

H

I

C

L

E

I

F

G

G

D

K

R

T

C

I

M

R

I

I

A

G

A

D

D

H

S

T

F

F

L

I

H

N

G

M

P

N

I

V

V

V

M

M

G

-

N

-

E

-

V

-

A

-

I

-

N

-

H

-

G

-

C

-

S

-

L

L

D

D

G

G

G

A

R

-

G

-

K

P

I

I

V

T

I

I

G

G

D

D

Q

H

T

V

R

L

I

I

A

G

N

P

N

S

V

T

T

Q

I

F

Y

Y

A

T

F

A

N

S

H

H

G

S

M

L

H

-

P

-

D

D

T

Y

P

R

I

R

Y

R

Q

Q

Q

A

-

W

S

E

S

T

Q

I

S

C

K

K

G

P

I

I

V

V

I

I

G

E

R

D

D

D

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W

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G

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G

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binding calcium ion: G138 (= G169), D156 (= D187)

3nz2C Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
31% identity, 79% coverage: 43:207/209 of query aligns to 33:183/183 of 3nz2C

query
sites
3nz2C

E

E

V

I

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N

Q

Q

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L

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D

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binding acetyl coenzyme *a: Y107 (= Y130), H111 (= H134), G137 (= G161), A155 (≠ G179), A156 (≠ M180), N161 (≠ T185), G171 (≠ A195), T173 (≠ N197), P174 (= P198), L178 (≠ I202), R179 (≠ G203)
binding magnesium ion: N157 (≠ G181), T173 (≠ N197)

Query Sequence

>5210146 FitnessBrowser__PV4:5210146
MQTPLANQVDYREQHKQRLAYMPWLYFSLKPKHLVWAKPWQDEVQQALMSVETVSIGKEC
FIAPQAKLFAEPGRDILIGDRCMIAADSFLHGPIVMGNEVAINHGCSLDGGRGKIVIGDQ
TRIANNVTIYAFNHGMHPDTPIYQQSSQSKGIVIGRDVWIGAQAGIVDGVTIGDCAVIGM
GCIVTKDVPDYAIVAGNPARIIGDRRDKK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory