SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 5210475 FitnessBrowser__PV4:5210475 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0A717 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Escherichia coli (strain K12) (see 4 papers)
83% identity, 97% coverage: 10:324/324 of query aligns to 1:315/315 of P0A717

query
sites
P0A717

V
x
M

P

P

D

D

I

M

K

K

L

L

F

F

A

A

G

G

N

N

A

A

T

T

P

P

S

E

L

L

A

A

K

Q

K

R

I

I

A

A

D

N

R

R

L

L

F

Y

C

T

K

S

L

L

G

G

D

D

A

A

E

A

V

V

G

G

V

R

F

F

S

S

D

D

G

G

E

E

I

V

S

S

V

V

Q

Q

I

I

N

N

E

E

N

N

V

V

R

R

G

G

A

G

D

D

V

I

F

F

I

I

Q

Q

S

S

T

T

C

C

A

A

P

P

T

T

N

N

D

D

N

N

L

L

M

M

E

E

L

L

I

V

V

V

M

M

V

V

D

D

A

A

L

L

R

R

A

A

S

S

A

A

G

G

R

R

I

I

T

T

A

A

V

V

I

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R

R

Q

Q

D

D

R

R

V

V

R

R

S

S

A

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

V

V

A

A

D

D

F

F

L

L

S

S

V

V

G

G

V

V

D

D

R

R

V

V

L

L

T

T

C

V

D
|
D

L

L

H

H

A

A

E

E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

V

V

P

P

V

V

D

D

N

N

V

V

F

F

G

G

S

S

P

P

V

I

L

L

E

E

D

D

M

M

L

L

A

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K

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N

L

L

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D

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N

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P

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S

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P

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D

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I

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G

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R

R

A

A

R

R

A

A

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A

A

K

K

L

L

D

N

D

D

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T

D

D

L

M

A

A

I

I

D

D

K

K

R

R

P

P

Q

R

A

A

N

N

V

V

A

S

Q

Q

V

V

M

M

H

H

I

I

G

G

D

D

V

V

Q

A

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G

R

R

D

D

C

C

I

V

I

L

V

V

D
|
D

M

M

I

I

D
|
D

T

T

G

G

T

T

L

L

C

C

K

K

A

A

E

E

A

A

L

L

K

K

E

E

H

R

G

G

A

A

N

K

R

R

V

V

F

F

A

A

Y

Y

A

A

T

T

H

H

P

P

V

I

F

F

S

S

G

G

N

N

A

A

P

A

K

N

N

N

I

L

A

R

E

N

S

S

V

V

I

I

D

D

E

E

V

V

I

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T

C

D

D

T

T

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I

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P

L

L

S

S

E

D

E

E

M

I

A

K

A

S

L

L

D

P

K

N

V

V

T

R

Q

T

L

L

T

T

M

L

S

S

S

G

V

M

M

L

A

A

E

E

A

A

I

I

R

R

V

I

S

S

N

N

E

E

S

S

I

I

S

S

A

A

M

M

F

F

K

E

H

H

M1 (≠ V10) modified: Initiator methionine, Removed
D129 (= D138) to A: in mutant PRSA1; alters the binding of divalent cations, especially magnesium. Little alteration in the affinity for ribose 5-phosphate and 27-fold decrease of the affinity for ATP. Absence of inhibition by AMP
D220 (= D229) mutation to E: 4-fold decrease in the affinity binding for Rib-5-P in the presence of magnesium ions. In the presence of cobalt ions, it shows a 15-fold decrease in the affinity binding for Rib-5-P.; mutation to F: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.
D221 (= D230) mutation to A: The affinity binding for ATP is comparable to those of the wild-type, apart from a slight decrease in the presence of manganese ions. The affinity binding for Rib-5-P is greatly decreased in the presence of both manganese and cobalt ions but only about 2-fold in the presence of magnesium ions.
D224 (= D233) mutation to A: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.; mutation to S: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.

6asvC E. Coli prpp synthetase (see paper)
83% identity, 96% coverage: 12:322/324 of query aligns to 1:311/311 of 6asvC

query
sites
6asvC

D

D

I

M

K

K

L

L

F

F

A

A

G

G

N

N

A

A

T

T

P

P

S

E

L

L

A

A

K

Q

K

R

I

I

A

A

D

N

R

R

L

L

F

Y

C

T

K

S

L

L

G

G

D

D

A

A

E

A

V

V

G

G

V

R

F

F

S

S

D

D

G

G

E

E

I

V

S

S

V

V

Q

Q

I

I

N

N

E

E

N

N

V

V

R

R

G

G

A

G

D

D

V

I

F

F

I

I

Q

Q

S

S

T

T

C

C

A

A

P

P

T

T

N

N

D

D

N

N

L

L

M

M

E

E

L

L

I

V

V

V

M

M

V

V

D

D

A

A

L

L

R

R

A

A

S

S

A

A

G

G

R

R

I

I

T

T

A

A

V

V

I

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R

R

Q

Q

D

D

R

R

V

V

R

R

S

S

A

A

R

R

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V

P

P

I

I

T

T

A

A

K

K

V

V

A

A

D

D

F

F

L

L

S

S

V

V

G

G

V

V

D

D

R

R

V

V

L

L

T

T

C

V

D

D

L

L

H

H

A

A

E

E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

V

V

P

P

V

V

D

D

N

N

V

V

F

F

G

G

S

S

P

P

V

I

L

L

E

E

D

D

M

M

L

L

A

Q

K

L

Q

N

L

L

D

D

N

N

P

P

V

I

V

V

S

S

P

P

D
|
D

I

I

G

G

V

V

R

R

A

A

R

R

A

A

V

I

A

A

K

K

L

L

D

N

D

D

S

T

D

D

L

M

A

A

I

I

D

D

K

K

R

R

P

P

Q

R

A

A

N

N

V

V

A

S

Q

Q

V

V

M

M

H

H

I

I

G

G

D

D

V

V

Q

A

G

G

R

R

D

D

C

C

I

V

I

L

V

V

D
|
D

M

M

I

I

D
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D

T
|
T

G
|
G

G

G

T
|
T

L
|
L

C

C

K

K

A

A

E

E

A

A

L

L

K

K

E

E

H

R

G

G

A

A

N

K

R

R

V

V

F

F

A

A

Y

Y

A

A

T

T

H

H

P

P

V

I

F

F

S

S

G

G

N

N

A

A

P

A

K

N

N

N

I

L

A

R

E

N

S

S

V

V

I

I

D

D

E

E

V

V

I

V

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V

T

C

D

D

T

T

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I

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P

L

L

S

S

E

D

E

E

M

I

A

K

A

S

L

L

D

P

K

N

V

V

T

R

Q

T

L

L

T

T

M

L

S

S

S

G

V

M

M

L

A

A

E

E

A

A

I

I

R

R

V

I

S

S

N

N

E

E

S

S

I

I

S

S

A

A

M

M

F

F

binding phosphate ion: D168 (= D179), D218 (= D229), D219 (= D230), D222 (= D233), T223 (= T234), G224 (= G235), T226 (= T237), L227 (= L238)

4s2uA Crystal structure of the phosphorybosylpyrophosphate synthetase from e. Coli
83% identity, 95% coverage: 11:318/324 of query aligns to 1:308/308 of 4s2uA

query
sites
4s2uA

P

P

D

D

I

M

K

K

L

L

F

F

A

A

G

G

N

N

A

A

T

T

P

P

S

E

L

L

A

A

K

Q

K

R

I

I

A

A

D

N

R

R

L

L

F

Y

C

T

K

S

L

L

G

G

D

D

A

A

E

A

V

V

G

G

V

R

F

F

S

S

D

D

G

G

E

E

I

V

S

S

V

V

Q

Q

I

I

N

N

E

E

N

N

V

V

R

R

G

G

A

G

D

D

V

I

F

F

I

I

Q

Q

S

S

T

T

C

C

A

A

P

P

T

T

N

N

D

D

N

N

L

L

M

M

E

E

L

L

I

V

V

V

M

M

V

V

D

D

A

A

L

L

R

R

A

A

S

S

A

A

G

G

R

R

I

I

T

T

A

A

V

V

I

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R

R

Q

Q

D

D

R

R

V

V

R

R

S

S

A

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

V

V

A

A

D

D

F

F

L

L

S

S

V

V

G

G

V

V

D

D

R

R

V

V

L

L

T

T

C

V

D

D

L

L

H

H

A

A

E

E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

V

V

P

P

V

V

D

D

N

N

V

V

F

F

G

G

S

S

P

P

V

I

L

L

E

E

D

D

M

M

L

L

A

Q

K

L

Q

N

L

L

D

D

N

N

P

P

V

I

V

V

S

S

P

P

D

D

I

I

G

G

V

V

R

R

A

A

R

R

A

A

V

I

A

A

K

K

L

L

D

N

D

D

S

T

D

D

L

M

A

A

I

I

D

D

K

K

R

R

P

P

Q

R

A

A

N

N

V

V

A

S

Q

Q

V

V

M

M

H

H

I

I

G

G

D

D

V

V

Q

A

G

G

R

R

D

D

C

C

I

V

I

L

V

V

D

D

M

M

I

I

D
|
D

T

T

G

G

T
|
T

L

L

C

C

K

K

A

A

E

E

A

A

L

L

K

K

E

E

H

R

G

G

A

A

N

K

R

R

V

V

F

F

A

A

Y

Y

A

A

T

T

H

H

P

P

V

I

F

F

S

S

G

G

N

N

A

A

P

A

K

N

N

N

I

L

A

R

E

N

S

S

V

V

I

I

D

D

E

E

V

V

I

V

V

V

T

C

D

D

T

T

I

I

P

P

L

L

S

S

E

D

E

E

M

I

A

K

A

S

L

L

D

P

K

N

V

V

T

R

Q

T

L

L

T

T

M

L

S

S

S

G

V

M

M

L

A

A

E

E

A

A

I

I

R

R

V

I

S

S

N

N

E

E

S

S

I

I

binding magnesium ion: D223 (= D233), T227 (= T237)

7xmvA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a(amp/adp) filament bound with adp, amp and r5p (see paper)
82% identity, 97% coverage: 12:324/324 of query aligns to 1:307/307 of 7xmvA

query
sites
7xmvA

D

D

I

M

K

K

L

L

F

F

A

A

G

G

N

N

A

A

T

T

P

P

S

E

L

L

A

A

K

Q

K

R

I

I

A

A

D

N

R

R

L

L

F

Y

C

T

K

S

L

L

G

G

D

D

A

A

E

A

V

V

G

G

V

R

F
|
F

S

S

D
|
D

G

G

E
|
E

I

V

S

S

V

V

Q

Q

I

I

N

N

E

E

N

N

V

V

R

R

G

G

A

G

D

D

V

I

F

F

I

I

Q

Q

S

S

T

T

C

C

A

A

P

P

T

T

N

N

D

D

N

N

L

L

M

M

E

E

L

L

I

V

V

V

M

M

V

V

D

D

A

A

L

L

R

R

A

A

S

S

A

A

G

G

R

R

I

I

T

T

A

A

V

V

I

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R
|
R

Q

Q

D

D

R
|
R

R

R

V
|
V

R
|
R

S

S

A

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

V

V

A

A

D

D

F

F

L

L

S

S

V

V

G

G

V

V

D

D

R

R

V

V

L

L

T

T

C

V

D

D

L

L

H
|
H

A

A

E
|
E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

V

V

P

P

V

V

D

D

N

N

V

V

F
|
F

G

G

S
|
S

P

P

V

I

L

L

E

E

D

D

M

M

L

L

A

Q

K

L

Q

N

L

L

D

D

N

N

P

P

V

I

V

V

S

S

P

P

D

D

I

I

G

G

V

V

V
|
V

R

R

A

A

R

R

A
|
A

V

I

A

A

K

K

L

L

D

N

D

D

S

T

D

D

L

M

A

A

I

I

D

D

K

K

R

R

P

-

Q

-

A

-

N

-

V

-

A

-

Q

Q

V

V

M

M

H

H

I

I

G

G

D

D

V

V

Q

A

G

G

R

R

D

D

C

C

I

V

I

L

V

V

D
|
D

M
|
M

I

I

D
|
D

T
|
T

G

G

G
|
G

T
|
T

L

L

C

C

K

K

A

A

E

E

A

A

L

L

K

K

E

E

H

R

G

G

A

A

N

K

R

R

V

V

F

F

A

A

Y

Y

A

A

T

T

H

H

P

P

V

I

F

F

S

S

G

G

N

N

A

A

P

A

K

N

N

N

I

L

A

R

E

N

S

S

V

V

I

I

D

D

E

E

V

V

I

V

V

V

T

C

D

D

T

T

I

I

P

P

L

L

S

S

E

D

E

E

M

I

A

K

A

S

L

L

D

P

K

N

V

V

T

R

Q

T

L

L

T

T

M

L

S

S

S

G

V

M

M

L

A

A

E

E

A

A

I

I

R

R

V

I

S

S

N

N

E

E

S

S

I

I

S

S

A

A

M

M

F

F

K

E

H

H

binding adenosine-5'-diphosphate: F33 (= F44), D35 (= D46), E37 (= E48), R94 (= R105), R97 (= R108), H129 (= H140)
binding adenosine monophosphate: R97 (= R108), V99 (= V110), R100 (= R111), E131 (= E142), F145 (= F156), S147 (= S158), V173 (= V184), A177 (= A188)
binding 5-O-phosphono-alpha-D-ribofuranose: D212 (= D229), D213 (= D230), M214 (= M231), D216 (= D233), T217 (= T234), G219 (= G236), T220 (= T237)

7xmuA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a filament bound with adp, pi and r5p (see paper)
82% identity, 97% coverage: 12:324/324 of query aligns to 1:307/307 of 7xmuA

query
sites
7xmuA

D

D

I

M

K

K

L

L

F

F

A

A

G

G

N

N

A

A

T

T

P

P

S

E

L

L

A

A

K

Q

K

R

I

I

A

A

D

N

R

R

L

L

F

Y

C

T

K

S

L

L

G

G

D

D

A

A

E

A

V

V

G

G

V

R

F
|
F

S

S

D
|
D

G

G

E
|
E

I

V

S

S

V

V

Q

Q

I

I

N

N

E

E

N

N

V

V

R

R

G

G

A

G

D

D

V

I

F

F

I

I

Q

Q

S

S

T

T

C

C

A

A

P

P

T

T

N

N

D

D

N

N

L

L

M

M

E

E

L

L

I

V

V

V

M

M

V

V

D

D

A

A

L

L

R

R

A

A

S

S

A

A

G

G

R

R

I

I

T

T

A

A

V

V

I

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

R

R

V

V

R
|
R

S

S

A

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

V

V

A

A

D

D

F

F

L

L

S

S

V

V

G

G

V

V

D

D

R

R

V

V

L

L

T

T

C

V

D

D

L

L

H
|
H

A

A

E
|
E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

V

V

P

P

V

V

D

D

N

N

V

V

F
|
F

G

G

S
|
S

P

P

V

I

L

L

E

E

D

D

M

M

L

L

A

Q

K

L

Q

N

L

L

D

D

N

N

P

P

V

I

V

V

S

S

P

P

D
|
D

I

I

G

G

V

V

V
|
V

R

R

A

A

R

R

A

A

V

I

A

A

K

K

L

L

D

N

D

D

S

T

D

D

L

M

A

A

I

I

D

D

K

K

R

R

P

-

Q

-

A

-

N

-

V

-

A

-

Q

Q

V

V

M

M

H

H

I

I

G

G

D

D

V

V

Q

A

G

G

R

R

D

D

C

C

I

V

I

L

V

V

D
|
D

D

D

M
|
M

I

I

D
|
D

T
|
T

G

G

T

T

L

L

C

C

K

K

A

A

E

E

A

A

L

L

K

K

E

E

H

R

G

G

A

A

N

K

R

R

V

V

F

F

A

A

Y

Y

A

A

T

T

H

H

P

P

V

I

F

F

S

S

G

G

N

N

A

A

P

A

K

N

N

N

I

L

A

R

E

N

S

S

V

V

I

I

D

D

E

E

V

V

I

V

V

V

T

C

D

D

T

T

I

I

P

P

L

L

S

S

E

D

E

E

M

I

A

K

A

S

L

L

D

P

K

N

V

V

T

R

Q

T

L

L

T

T

M

L

S

S

S

G

V

M

M

L

A

A

E

E

A

A

I

I

R

R

V

I

S

S

N

N

E

E

S

S

I

I

S

S

A

A

M

M

F

F

K

E

H

H

binding adenosine-5'-diphosphate: F33 (= F44), D35 (= D46), E37 (= E48), R94 (= R105), Q95 (= Q106), R97 (= R108), R97 (= R108), R100 (= R111), H129 (= H140), E131 (= E142), F145 (= F156), S147 (= S158), V173 (= V184)
binding 5-O-phosphono-alpha-D-ribofuranose: D168 (= D179), D212 (= D229), M214 (= M231), D216 (= D233), T217 (= T234)

Q63XL8 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Burkholderia pseudomallei (strain K96243) (see paper)
63% identity, 96% coverage: 13:322/324 of query aligns to 7:315/318 of Q63XL8

query
sites
Q63XL8

I

L

K

M

L

V

F

F

A

T

G

G

N

N

A

A

T

N

P

P

S

A

L

L

A

A

K

Q

K

E

I

V

A

V

D

K

R

I

L

L

F

G

C

I

K

P

L

L

G

G

D

K

A

A

E

M

V

V

G

S

V

R

F

F

S

S

D

D

G

G

E

E

I

I

S

Q

V

V

Q

E

I

I

N

Q

E

E

N

N

V

V

R

R

G

G

A

K

D

D

V

V

F

F

I

V

I

L

Q

Q

S

S

T

T

C

C

A

A

P

P

T

T

N

N

D

D

N

N

L

L

M

M

E

E

L

L

I

M

V

I

M

M

V

V

D

D

A

A

L

L

R

K

R

R

A

A

S

S

A

A

G

G

R

R

I

I

T

T

A

A

V

A

I

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

V

P

R

R

S

S

A

A

R

R

V

V

P

A

I

I

T

S

A

A

K

K

V

V

A

A

D

N

F

M

L

L

S

E

S

I

V

A

G

G

V

V

D

E

R

R

V

I

L

I

T

T

C

M

D

D

L

L

H

H

A

A

E

D

Q

Q

I

I

Q

Q

G

G

F

F

D

D

V

I

P

P

V

V

D

D

N

N

V

I

F

Y

G

A

S

T

P

P

V

I

L

L

E

G

D

D

M

L

L

R

A

K

K

Q

Q

N

L

Y

D

P

N

D

P

L

V

L

V

V

S

S

P

P

D

D

I

V

G

G

V

V

R

R

A

A

R

R

A

A

V

L

A

A

K

K

L

Q

L

L

D

-

D

N

S

C

D

D

L

L

A

A

I

I

D

D

K

K

R

R

P

P

Q

K

A

A

N

N

V

V

A

A

Q

E

V

V

M

M

H

N

I

I

G

G

D

E

V

V

Q

E

G

G

R

R

D

T

C

C

I

V

I

I

V

M

D

D

M

M

I

V

D

D

T

T

G

A

G

G

T

T

L

L

C

C

K

K

A

A

E

Q

A

V

L

L

K

K

E

E

H

R

G

G

A

A

N

K

R

Q

V

V

F

F

A

A

Y

Y

A

A

T

T

H

H

P

P

V

V

F

L

S

S

G

G

N

G

A

A

P

A

K

D

N

R

I

I

A

A

E

A

S

S

V

A

I

L

D

D

E

E

V

L

I

V

V

V

T

T

D

D

T

T

I

I

P

P

L

L

S

S

E

A

E

E

M

S

A

L

A

A

L

C

D

P

K

K

V

I

T

R

Q

A

L

L

T

S

M

S

S

A

S

G

V

L

M

L

A

A

E

E

A

T

I

F

R

S

R

R

V

I

S

R

N

R

E

G

E

D

S

S

I

V

S

M

A

S

M

L

F

F

RQ 99:100 (= RQ 105:106) binding

6nfeB Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
64% identity, 92% coverage: 15:313/324 of query aligns to 5:298/299 of 6nfeB

query
sites
6nfeB

L

I

F

F

A

T

G

G

N

N

A

A

T

N

P

P

S

E

L

L

A

A

K

L

K

K

I

I

A

S

D

S

R

H

L

L

F

Q

C

I

K

P

L

I

G

G

D

K

A

A

E

T

V

V

G

G

V

T

F
|
F

S

S

D
|
D

G

G

E
|
E

I

T

S

M

V

V

Q

E

I

I

N

L

E

E

N

N

V

V

R

R

G

G

A

K

D

D

V

V

F

F

I

V

I

L

Q

Q

S

S

T

T

C

C

A

A

P

P

T

A

N

N

D

N

N

N

L

L

M

M

E

E

L

L

I

L

V

I

M

M

V

A

D

D

A

A

L

L

R

R

A

S

S

S

A

A

G

G

R

R

I

I

T

T

A

A

V

V

I

V

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

V

V

R

R

S

S

A

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

V

V

A

A

D

D

F

M

L

M

S

A

S

S

V

V

G

G

V

I

D

C

R

R

V

V

L

L

T

T

C

V

D

D

L

L

H
|
H

A

A

E

D

Q

Q

I

I

Q

Q

G

G

F

F

D

Y

V

M

P

P

V

V

D

D

N

N

V

V

F

Y

G

S

S

T

P

P

V

V

L

L

E

E

D

D

M

I

L

T

A

K

K

Q

Q

K

L

L

D

N

N

N

P

I

V

M

V

I

V

V

S

S

P

P

D

D

I

V

G

G

V

V

R

R

A

A

R

R

A

A

V

V

A

A

K

K

L

R

L

L

D

N

D

D

S

A

D

E

L

L

A

S

I

I

D

D

K

K

R

R

P

-

Q

-

A

-

N

-

V

-

A

S

Q

E

V

V

M

M

H

H

I

I

G

G

D

E

V

P

Q

A

G

N

R

K

D

N

C

C

I

I

V

V

D
|
D

M
x
I

I

V

D
|
D

T
|
T

G
x
A

G

G

T
|
T

L

L

C

C

K

T

A

A

E

H

A

E

L

L

K

K

E

K

H

N

G

G

A

A

N

K

R

S

V

V

F

R

A

A

Y

Y

A

I

T

T

H

H

P

P

V

V

F

L

S

S

G

G

N

P

A

A

P

V

K

N

N

N

I

I

A

K

E

H

S

S

V

G

I

L

D

D

E

E

V

V

I

V

V

V

T

T

D

D

T

T

I

I

P

P

L

L

S

S

E

A

E

E

M

A

A

Q

A

N

L

C

D

E

K

K

V

I

T

R

Q

V

L

V

T

S

M

L

S

A

S

D

V

M

M

L

A

A

E

Q

A

A

I

I

R

K

R

R

V

V

S

N

binding adenosine-5'-diphosphate: F34 (= F44), D36 (= D46), E38 (= E48), R95 (= R105), Q96 (= Q106), H130 (= H140)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H140), D214 (= D229), D215 (= D230), I216 (≠ M231), D218 (= D233), T219 (= T234), A220 (≠ G235), T222 (= T237)

6nfeA Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
64% identity, 92% coverage: 15:313/324 of query aligns to 5:297/298 of 6nfeA

query
sites
6nfeA

L

I

F

F

A

T

G

G

N

N

A

A

T

N

P

P

S

E

L

L

A

A

K

L

K

K

I

I

A

S

D

S

R

H

L

L

F

Q

C

I

K

P

L

I

G

G

D

K

A

A

E

T

V

V

G

G

V

T

F
|
F

S

S

D
|
D

G

G

E
|
E

I

T

S

M

V

V

Q

E

I

I

N

L

E

E

N

N

V

V

R

R

G

G

A

K

D

D

V

V

F

F

I

V

I

L

Q

Q

S

S

T

T

C

C

A

A

P

P

T

A

N

N

D

N

N

N

L

L

M

M

E

E

L

L

I

L

V

I

M

M

V

A

D

D

A

A

L

L

R

R

A

S

S

S

A

A

G

G

R

R

I

I

T

T

A

A

V

V

I

V

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

R

R

V
|
V

R

R

S

S

A

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

V

V

A

A

D

D

F

M

L

M

S

A

S

S

V

V

G

G

V

I

D

C

R

R

V

V

L

L

T

T

C

V

D

D

L

L

H
|
H

A

A

E

D

Q

Q

I

I

Q

Q

G

G

F

F

D

Y

V

M

P

P

V

V

D

D

N

N

V

V

F
x
Y

G

S

S

T

P

P

V

V

L

L

E

E

D

D

M

I

L

T

A

K

K

Q

Q

K

L

L

D

N

N

N

P

I

V

M

V

I

V

V

S

S

P

P

D

D

I

V

G

G

V

V

R
|
R

A

A

R

R

A
|
A

V

V

A

A

K
|
K

L

R

L

L

D

N

D

D

S

A

D

E

L

L

A

S

I

I

D

D

K

K

R

R

P

-

Q

-

A

-

N

-

V

-

A

-

Q

E

V

V

M

M

H

H

I

I

G

G

D

E

V

P

Q

A

G

N

R

K

D

N

C

C

I

I

V

V

D
|
D

M
x
I

I

V

D
|
D

T
|
T

G
x
A

G

G

T
|
T

L

L

C

C

K

T

A

A

E

H

A

E

L

L

K

K

E

K

H

N

G

G

A

A

N

K

R

S

V

V

F

R

A

A

Y

Y

A

I

T

T

H

H

P

P

V

V

F

L

S

S

G

G

N

P

A

A

P

V

K

N

N

N

I

I

A

K

E

H

S

S

V

G

I

L

D

D

E

E

V

V

I

V

V

V

T

T

D

D

T

T

I

I

P

P

L

L

S

S

E

A

E

E

M

A

A

Q

A

N

L

C

D

E

K

K

V

I

T

R

Q

V

L

V

T

S

M

L

S

A

S

D

V

M

M

L

A

A

E

Q

A

A

I

I

R

K

R

R

V

V

S

N

binding adenosine-5'-diphosphate: F34 (= F44), D36 (= D46), E38 (= E48), R95 (= R105), Q96 (= Q106), H130 (= H140)
binding adenosine monophosphate: R98 (= R108), V100 (= V110), Y146 (≠ F156), R175 (= R185), A178 (= A188), K181 (= K191)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H140), D213 (= D229), D214 (= D230), I215 (≠ M231), D217 (= D233), T218 (= T234), A219 (≠ G235), T221 (= T237)

3dahC 2.3 a crystal structure of ribose-phosphate pyrophosphokinase from burkholderia pseudomallei (see paper)
61% identity, 94% coverage: 13:318/324 of query aligns to 2:299/300 of 3dahC

query
sites
3dahC

I

L

K

M

L

V

F

F

A

T

G

G

N

N

A

A

T

N

P

P

S

A

L

L

A

A

K

Q

K

E

I

V

A

V

D

K

R

I

L

L

F

G

C

I

K

P

L

L

G

G

D

K

A

A

E

M

V

V

G

S

V

R

F
|
F

S

S

D
|
D

G

G

E
|
E

I

I

S

Q

V

V

Q

E

I

I

N

Q

E

E

N

N

V

V

R

R

G

G

A

K

D

D

V

V

F

F

I

V

I

L

Q

Q

S

S

T

T

C

C

A

A

P

P

T

T

N

N

D

D

N

N

L

L

M

M

E

E

L

L

I

M

V

I

M

M

V

V

D

D

A

A

L

L

R

K

R

R

A

A

S

S

A

A

G

G

R

R

I

I

T

T

A

A

V

A

I

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

V

P

R

R

S

S

A

A

R

R

V

V

P

A

I

I

T

S

A

A

K

K

V

V

A

A

D

N

F

M

L

L

S

E

S

I

V

A

G

G

V

V

D

E

R

R

V

I

L

I

T

T

C

M

D

D

L

L

H

H

A

A

E

D

Q

Q

I

I

Q

Q

G

G

F

F

D

D

V

I

P

P

V

V

D

D

N

N

V

I

F

Y

G

A

S

T

P

P

V

I

L

L

E

G

D

D

M

L

L

R

A

K

K

Q

Q

N

L

Y

D

P

N

D

P

L

V

L

V

V

S

S

P

P

D

D

I

V

G

G

V

V

R

R

A

A

R

R

A

A

V

L

A

A

K

K

L

Q

L

L

D

-

D

N

S

C

D

D

L

L

A

A

I

I

D

D

K

K

R

R

P

-

Q

-

A

-

N

-

V

-

A

-

Q

-

V

V

M

M

H

N

I

I

G

G

D

E

V

V

Q

E

G

G

R

R

D

T

C

C

I

V

I

I

V

M

D

D

M

M

I

V

D

D

T

T

G

A

G

G

T

T

L

L

C

C

K

K

A

A

E

Q

A

V

L

L

K

K

E

E

H

R

G

G

A

A

N

K

R

Q

V

V

F

F

A

A

Y

Y

A

A

T

T

H

H

P

P

V

V

F

L

S

S

G

G

N

G

A

A

P

A

K

D

N

R

I

I

A

A

E

A

S

S

V

A

I

L

D

D

E

E

V

L

I

V

V

V

T

T

D

D

T

T

I

I

P

P

L

L

S

S

E

A

E

E

M

S

A

L

A

A

L

C

D

P

K

K

V

I

T

R

Q

A

L

L

T

S

M

S

S

A

S

G

V

L

M

L

A

A

E

E

A

T

I

F

R

S

R

R

V

I

S

R

N

R

E

G

E

D

S

S

I

V

binding adenosine monophosphate: F33 (= F44), D35 (= D46), E37 (= E48), R94 (= R105), Q95 (= Q106)

P14193 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PPRibP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Bacillus subtilis (strain 168) (see 4 papers)
51% identity, 96% coverage: 12:322/324 of query aligns to 9:316/317 of P14193

query
sites
P14193

D

N

I

L

K

K

L

I

F

F

A

S

G

L

N

N

A

S

T

N

P

P

S

E

L

L

A

A

K

K

K

E

I

I

A

A

D

D

R

I

L

V

F

G

C

V

K

Q

L

L

G

G

D

K

A

C

E

S

V

V

G

T

V

R

F

F

S

S

D

D

G

G

E

E

I

V

S

Q

V

I

Q

N

I

I

N

E

E

E

N

S

V

I

R

R

G

G

A

C

D

D

V

C

F

Y

I

I

Q

Q

S

S

T

T

C

S

A

D

P

P

T

V

N

N

D

E

N

H

L

I

M

M

E

E

L

L

I

L

V

I

M

M

V

V

D

D

A

A

L

L

R

K

R

R

A

A

S

S

A

A

G

K

R

T

I

I

T

N

A

I

V

V

I

I

P

P

Y

Y

F

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

R

K

V

A

R

R

S

S

A

-

R

R

V

E

P

P

I

I

T

T

A

A

K

K

V

L

V

F

A

A

D

N

F

L

L

L

S

E

S

T

V

A

G

G

V

A

D

T

R

R

V

V

L

I

T

A

C

L

D

D

L

L

H

H

A

A

E

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

V

I

P

P

V

I

D

D

N

H

V

L

F

M

G

G

S

V

P

P

V

I

L

L

L

G

E

E

D

Y

M

F

L

E

A

G

K

K

Q

N

L

L

D

E

N

D

P

I

V

V

V

I

V

V

S

S

P

P

D

D

I

H

G

G

V

V

V

T

R

R

A

A

R

R

A

K

V

L

A

A

K

D

L

R

L

L

D

-

D

K

S

A

D

P

L

I

A

A

I

I

D

D

K
|
K

R

R

R
|
R

P

P

Q
x
R

A

P

N
|
N

V

V

A

A

Q
x
E

V

V

M

M

H

N

I

I

I

V

G

G

D

N

V

I

Q

E

G

G

R

K

D

T

C

A

I

I

I

L

V

I

D

D

M

I

I

I

D
|
D

T
|
T

G
x
A

G
|
G

T
|
T

L

I

C

T

K

L

A

A

E

N

A

A

L

L

K

V

E

E

H

N

G

G

A

A

N

K

R

E

V

V

F

Y

A

A

Y

C

A

C

T

T

H

H

P

P

V

V

F

L

S

S

G

G

N

P

A

A

P

V

K

E

N

R

I

I

A

N

E

N

S

S

V

T

I

I

D

K

E

E

V

L

I

V

V

V

T

T

D

N

T

S

I

I

P

K

L

L

S

P

E

E

M

-

A

K

L

I

D

E

K

R

V

F

T

K

Q

Q

L

L

T

S

M

V

S

G

S

P

V

L

M

L

A

A

E

E

A

A

I

I

R

I

R

R

V

V

S

H

N

E

E

Q

S

S

I

V

S

S

A

Y

M

L

F

F

RQ 102:103 (= RQ 105:106) binding
K198 (= K203) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type. The cooperativity of ADP binding is reduced.
R200 (= R205) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type enzyme. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type.
R202 (≠ Q207) mutation to A: 3-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
N204 (= N209) mutation to A: 4.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
E207 (≠ Q212) mutation to A: 2.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
DTAGT 228:232 (≠ DTGGT 233:237) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1ibsB Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
49% identity, 96% coverage: 12:322/324 of query aligns to 3:299/299 of 1ibsB

query
sites
1ibsB

D

N

I

L

K

K

L

I

F

F

A

S

G

L

N

N

A

S

T

N

P

P

S

E

L

L

A

A

K

K

K

E

I

I

A

A

D

D

R

I

L

V

F

G

C

V

K

Q

L

L

G

G

D

K

A

C

E

S

V

V

G

T

V

R

F

F

S

S

D

D

G

G

E

E

I

V

S

Q

V

I

Q

N

I

I

N

E

E

E

N

S

V

I

R

R

G

G

A

C

D

D

V

C

F

Y

I

I

Q

Q

S

S

T

T

C

S

A

D

P

P

T

V

N

N

D

E

N

H

L

I

M

M

E

E

L

L

I

L

V

I

M

M

V

V

D

D

A

A

L

L

R

K

R

R

A

A

S

S

A

A

G

K

R

T

I

I

T

N

A

I

V

V

I

I

P

P

Y

Y

F

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

R

K

V

-

R

-

S

-

A

S

R

R

V

E

P

P

I

I

T

T

A

A

K

K

V

L

V

F

A

A

D

N

F

L

L

L

S

E

S

T

V

A

G

G

V

A

D

T

R

R

V

V

L

I

T

A

C

L

D

D

L

L

H

H

A

A

E

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

V

I

P

P

V

I

D

D

N

H

V

L

F

M

G

G

S

V

P

P

V

I

L

L

L

G

E

E

D

Y

M

F

L

E

A

G

K

K

Q

N

L

L

D

E

N

D

P

I

V

V

V

I

V

V

S

S

P

P

D

D

I

H

G

G

V

V

V

T

R

R

A

A

R

R

A

K

V

L

A

A

K

D

L

R

L

L

D

-

D

K

S

A

D

P

L

I

A

A

I

I

D

D

K

K

R

R

R

-

P

-

Q

-

A

-

N

-

V

-

A

-

Q

-

V

-

M

M

H

N

I

I

I

V

G

G

D

N

V

I

Q

E

G

G

R

K

D

T

C

A

I

I

I

L

V

I

D

D

M

I

I

I

D

D

T

T

G

A

G

G

T

T

L

I

C

T

K

L

A

A

E

N

A

A

L

L

K

V

E

E

H

N

G

G

A

A

N

K

R

E

V

V

F

Y

A

A

Y

C

A

C

T

T

H

H

P

P

V

V

F

L

S

S

G

G

N

P

A

A

P

V

K

E

N

R

I

I

A

N

E

N

S

S

V

T

I

I

D

K

E

E

V

L

I

V

V

V

T

T

D

N

T

S

I

I

P

K

L

L

S

P

E

E

M

-

A

K

L

I

D

E

K

R

V

F

T

K

Q

Q

L

L

T

S

M

V

S

G

S

P

V

L

M

L

A

A

E

E

A

A

I

I

R

I

R

R

V

V

S

H

N

E

E

Q

S

S

I

V

S

S

A

Y

M

L

F

F

binding methyl phosphonic acid adenosine ester: R96 (= R105), Q97 (= Q106), R99 (= R108)

1ibsA Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
49% identity, 96% coverage: 12:322/324 of query aligns to 1:297/297 of 1ibsA

query
sites
1ibsA

D

N

I

L

K

K

L

I

F

F

A

S

G

L

N

N

A

S

T

N

P

P

S

E

L

L

A

A

K

K

K

E

I

I

A

A

D

D

R

I

L

V

F

G

C

V

K

Q

L

L

G

G

D

K

A

C

E

S

V

V

G

T

V

R

F

F

S

S

D

D

G

G

E

E

I

V

S

Q

V

I

Q

N

I

I

N

E

E

E

N

S

V

I

R

R

G

G

A

C

D

D

V

C

F

Y

I

I

Q

Q

S

S

T

T

C

S

A

D

P

P

T

V

N

N

D

E

N

H

L

I

M

M

E

E

L

L

I

L

V

I

M

M

V

V

D

D

A

A

L

L

R

K

R

R

A

A

S

S

A

A

G

K

R

T

I

I

T

N

A

I

V

V

I

I

P

P

Y

Y

F

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

R

K

V

-

R

-

S

-

A

S

R

R

V

E

P

P

I

I

T

T

A

A

K

K

V

L

V

F

A

A

D

N

F

L

L

L

S

E

S

T

V

A

G

G

V

A

D

T

R

R

V

V

L

I

T

A

C

L

D

D

L

L

H
|
H

A

A

E

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

V

I

P

P

V

I

D

D

N

H

V

L

F

M

G

G

S

V

P

P

V

I

L

L

L

G

E

E

D

Y

M

F

L

E

A

G

K

K

Q

N

L

L

D

E

N

D

P

I

V

V

V

I

V

V

S

S

P

P

D

D

I

H

G

G

V

V

V

T

R

R

A

A

R

R

A

K

V

L

A

A

K

D

L

R

L

L

D

-

D

K

S

A

D

P

L

I

A

A

I

I

D

D

K

K

R

R

R

-

P

-

Q

-

A

-

N

-

V

-

A

-

Q

-

V

-

M

M

H

N

I

I

I

V

G

G

D

N

V

I

Q

E

G

G

R

K

D

T

C

A

I

I

I

L

V

I

D

D

M

I

I

I

D
|
D

T
|
T

G
x
A

G

G

T
|
T

L

I

C

T

K

L

A

A

E

N

A

A

L

L

K

V

E

E

H

N

G

G

A

A

N

K

R

E

V

V

F

Y

A

A

Y

C

A

C

T

T

H

H

P

P

V

V

F

L

S

S

G

G

N

P

A

A

P

V

K

E

N

R

I

I

A

N

E

N

S

S

V

T

I

I

D

K

E

E

V

L

I

V

V

V

T

T

D

N

T

S

I

I

P

K

L

L

S

P

E

E

M

-

A

K

L

I

D

E

K

R

V

F

T

K

Q

Q

L

L

T

S

M

V

S

G

S

P

V

L

M

L

A

A

E

E

A

A

I

I

R

I

R

R

V

V

S

H

N

E

E

Q

S

S

I

V

S

S

A

Y

M

L

F

F

binding methyl phosphonic acid adenosine ester: R94 (= R105), Q95 (= Q106), R97 (= R108), H126 (= H140)
binding sulfate ion: D209 (= D233), T210 (= T234), A211 (≠ G235), T213 (= T237)

1dkuA Crystal structures of bacillus subtilis phosphoribosylpyrophosphate synthetase: molecular basis of allosteric inhibition and activation. (see paper)
49% identity, 96% coverage: 12:322/324 of query aligns to 1:295/295 of 1dkuA

query
sites
1dkuA

D

N

I

L

K

K

L

I

F

F

A

S

G

L

N

N

A

S

T

N

P

P

S

E

L

L

A

A

K

K

K

E

I

I

A

A

D

D

R

I

L

V

F

G

C

V

K

Q

L

L

G

G

D

K

A

C

E

S

V

V

G

T

V

R

F

F

S

S

D

D

G

G

E

E

I

V

S

Q

V

I

Q

N

I

I

N

E

E

E

N

S

V

I

R

R

G

G

A

C

D

D

V

C

F

Y

I

I

Q

Q

S

S

T

T

C

S

A

D

P

P

T

V

N

N

D

E

N

H

L

I

M

M

E

E

L

L

I

L

V

I

M

M

V

V

D

D

A

A

L

L

R

K

R

R

A

A

S

S

A

A

G

K

R

T

I

I

T

N

A

I

V

V

I

I

P

P

Y

Y

F

Y

G

G

Y

Y

A

A

R
|
R

Q

Q

D

D

R

R

R
x
K

V

A

R

R

S
|
S

A

-

R
|
R

V

E

P

P

I

I

T

T

A

A

K

K

V

L

V

F

A

A

D

N

F

L

L

L

S

E

S

T

V

A

G

G

V

A

D

T

R

R

V

V

L

I

T

A

C

L

D

D

L

L

H
|
H

A

A

E

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

V

I

P

P

V

I

D
|
D

N

H

V

L

F

M

G

G

S

V

P

P

V

I

L

L

L

G

E

E

D

Y

M

F

L

E

A

G

K

K

Q

N

L

L

D

E

N

D

P

I

V

V

V

I

V

V

S

S

P

P

D

D

I

H

G

G

V

V

V

T

R

R

A

A

R

R

A

K

V

L

A

A

K

D

L

R

L

L

D

-

D

K

S

A

D

P

L

I

A

A

I

I

D

D

K

K

R

R

R

-

P

-

Q

-

A

-

N

-

V

-

A

-

Q

-

V

-

M

M

H

N

I

I

I

V

G

G

D

N

V

I

Q

E

G

G

R

K

D

T

C

A

I

I

I

L

V

I

D

D

M

I

I

I

D

D

T

T

G

A

G

G

T

T

L

I

C

T

K

L

A

A

E

N

A

A

L

L

K

V

E

E

H

N

G

G

A

A

N

K

R

E

V

V

F

Y

A

A

Y

C

A

C

T

T

H

H

P

P

V

V

F

L

S

S

G

G

N

P

A

A

P

V

K

E

N

R

I

I

A

N

E

N

S

S

V

T

I

I

D

K

E

E

V

L

I

V

V

V

T

T

D

N

T

S

I

I

P

K

L

L

S

K

E

-

M

-

A

-

L

I

D

E

K

R

V

F

T

K

Q

Q

L

L

T

S

M

V

S

G

S

P

V

L

M

L

A

A

E

E

A

A

I

I

R

I

R

R

V

V

S

H

N

E

E

Q

S
|
S

I
x
V

S
|
S

A

Y

M

L

F
|
F

binding methyl phosphonic acid adenosine ester: R94 (= R105), H128 (= H140)
binding phosphomethylphosphonic acid adenosyl ester: K98 (≠ R109), S101 (= S112), R102 (= R114), D141 (= D153), S290 (= S317), V291 (≠ I318), S292 (= S319), F295 (= F322)

P60891 Ribose-phosphate pyrophosphokinase 1; PPRibP; Phosphoribosyl pyrophosphate synthase I; PRS-I; EC 2.7.6.1 from Homo sapiens (Human) (see 5 papers)
48% identity, 97% coverage: 10:324/324 of query aligns to 1:315/318 of P60891

query
sites
P60891

V
x
M

P

P

D

N

I

I

K

K

L

I

F

F

A

S

G

G

N

S

A

S

T

H

P

Q

S

D

L

L

A
x
S

K

Q

K

K

I

I

A

A

D

D

R

R

L

L

F

G

C

L

K

E

L

L

G

G

D

K

A

V

E

V

V

T

G

K

V

K

F

F

S

S

D

N

G

Q

E

E

I

T

S

C

V

V

Q

E

I

I

N

G

E

E

N

S

V

V

R

R

G

G

A

E

D
|
D

V

V

F

Y

I

I

I

V

Q

Q

S

S

T

G

C

C

A

G

P

E

T

I

N

N

D

D

N

N

L

L

M

M

E

E

L

L

I

L

V

I

M

M

V

I

D

N

A

A

L

C

R

K

R

I

A

A

S

S

A

A

G

S

R

R

I

V

T

T

A

A

V

V

I

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R

R

Q

Q

D

D

R

K

V

D

R

K

S

S

A

-

R

R

V

A

P

P

I

I

T

S

A

A

K

K

V

L

V

V

A

A

D
x
N

F

M

L

L

S

S

S

V

V

A

G

G

V

A

D

D

R

H

V

I

L

I

T

T

C

M

D

D

L
|
L

H

H

A

A

E
x
S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

V

I

P

P

V
|
V

D

D

N
|
N

V

L

F
x
Y

G

A

S

E

P

P

V

A

L

V

L

L

E

K

D

W

M

I

L

R

A

E

K

N

-

I

-

S

Q

E

L

W

D

R

N

N

P

C

V

T

V

I

V

V

S

S

P

P

D

D

I

A

G

G

V

A

V

K

R

R

A

V

R

T

A

S

V

I

A

A

K
x
D

L

R

L

L

D

N

D

-

S

V

D

D

L

F

A
|
A

I

L

I

I

D
x
H

K

K

R

E

R

R

P

K

Q

K

A

A

N

N

V

E

A

V

Q
x
D

V

R

M

M

H

V

I

L

I

V

G

G

D

D

V

V

Q

K

G

D

R

R

D

V

C

A

I

I

I

L

V
|
V

D

D

M

M

I

A

D

D

T

T

G

C

G

G

T

T

L

I

C

C

K
x
H

A

A

E

D

A

K

L

L

K

L

E

S

H

A

G

G

A

A

N

T

R

R

V

V

F

Y

A

A

Y

I

A

L

T

T

H

H

P

G

V

I

F

F

S

S

G

G

N

P

A

A

P

I

K

S

N

R

I

I

A

N

E

N

S

A

V

C

I

F

D

E

E

A

V

V

I

V

V

V

T

T

D

N

T

T

I

I

P

P

L

Q

S

E

E

D

E

K

M

M

A

K

A

H

L

C

D

S

K

K

V

I

T

Q

Q

V

L

I

T

D

M

I

S

S

S

M

V

I

M

L

A

A

E

E

A

A

I

I

R

R

V

T

S

H

N

N

E

G

E

E

S

S

I

V

S

S

A

Y

M

L

F

F

K

S

H

H

M1 (≠ V10) modified: Initiator methionine, Removed
S16 (≠ A25) to P: found in patients with phosphoribosyl pyrophosphate synthetase I deficiency; likely pathogenic; dbSNP:rs869025594
D52 (= D61) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852542
N114 (≠ D124) to S: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852540
L129 (= L139) to I: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852543
S132 (≠ E142) mutation to A: Reduces catalytic activity.; mutation to F: No effect on catalytic activity.
V142 (= V152) to L: found in a patient with an intermediate phenotype between ARTS and PRPS1 superactivity; likely pathogenic; normal PRPP synthetase activity in fibroblasts; loss of activity in erythrocytes; dbSNP:rs398122855
N144 (= N154) mutation to H: No effect on catalytic activity.
Y146 (≠ F156) mutation to F: No effect on catalytic activity.; mutation to M: Reduces catalytic activity.
D183 (≠ K191) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852541
A190 (= A199) to V: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852544
H193 (≠ D202) to Q: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852545
D203 (≠ Q212) to H: in a breast cancer sample; somatic mutation
V219 (= V228) to G: in a breast cancer sample; somatic mutation
H231 (≠ K240) to D: in a colorectal cancer sample; somatic mutation

8dbkB Human prps1 with phosphate, atp, and r5p; hexamer with resolved catalytic loops (see paper)
48% identity, 97% coverage: 11:324/324 of query aligns to 1:314/316 of 8dbkB

query
sites
8dbkB

P

P

D

N

I

I

K

K

L

I

F

F

A

S

G

G

N

S

A

S

T

H

P

Q

S

D

L

L

A

S

K

Q

K

K

I

I

A

A

D

D

R

R

L

L

F

G

C

L

K

E

L

L

G

G

D

K

A

V

E

V

V

T

G

K

V

K

F
|
F

S

S

D
x
N

G

Q

E
|
E

I

T

S

C

V

V

Q

E

I

I

N

G

E

E

N
x
S

V

V

R
|
R

G

G

A

E

D

D

V

V

F

Y

I

I

I

V

Q

Q

S

S

T

G

C

C

A

G

P

E

T

I

N

N

D

D

N

N

L

L

M

M

E

E

L

L

I

L

V

I

M

M

V

I

D

N

A

A

L

C

R

K

R

I

A

A

S

S

A

A

G

S

R

R

I

V

T

T

A

A

V

V

I

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

K

V

D

R

K

S

S

A

-

R

R

V

A

P

P

I

I

T

S

A

A

K

K

V

L

V

V

A

A

D

N

F

M

L

L

S

S

S

V

V

A

G

G

V

A

D

D

R

H

V

I

L

I

T

T

C

M

D

D

L

L

H
|
H

A

A

E

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

V

I

P

P

V

V

D

D

N

N

V

L

F

Y

G

A

S

E

P

P

V

A

L

V

L

L

E

K

D

W

M

I

L

R

A

E

K

N

-

I

-

S

Q

E

L

W

D

R

N

N

P

C

V

T

V

I

V

V

S

S

P

P

D
|
D

I

A

G
|
G

G

G

V

A

V

K

R

R

A

V

R

T

A

S

V

I

A

A

K

D

L

R

L

L

D

N

D

-

S

V

D

D

L

F

A

A

I

L

I

I

D

H

K
|
K

R

E

R
|
R

P

K

Q

K

A

A

N
|
N

V

E

A

V

Q

D

V

R

M

M

H

V

I

L

I

V

G

G

D

D

V

V

Q

K

G

D

R

R

D

V

C

A

I

I

I

L

V

V

D
|
D

M

M

I

A

D
|
D

T
|
T

G
x
C

G
|
G

T
|
T

L

I

C

C

K

H

A

A

E

D

A

K

L

L

K

L

E

S

H

A

G

G

A

A

N

T

R

R

V

V

F

Y

A

A

Y

I

A

L

T

T

H

H

P

G

V

I

F

F

S

S

G

G

N

P

A

A

P

I

K

S

N

R

I

I

A

N

E

N

S

A

V

C

I

F

D

E

E

A

V

V

I

V

V

V

T

T

D

N

T

T

I

I

P

P

L

Q

S

E

E

D

E

K

M

M

A

K

A

H

L

C

D

S

K

K

V

I

T

Q

Q

V

L

I

T

D

M

I

S

S

S

M

V

I

M

L

A

A

E

E

A

A

I

I

R

R

V

T

S

H

N

N

E

G

E

E

S

S

I

V

S

S

A

Y

M

L

F

F

K

S

H

H

binding adenosine monophosphate: R95 (= R105), Q96 (= Q106), N199 (= N209)
binding adenosine-5'-triphosphate: F34 (= F44), N36 (≠ D46), E38 (= E48)
binding phosphate ion: S46 (≠ N56), R48 (= R58)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: H129 (= H140), D170 (= D179), G172 (= G181), K193 (= K203), R195 (= R205), D219 (= D229), D220 (= D230), D223 (= D233), T224 (= T234), C225 (≠ G235), G226 (= G236), T227 (= T237)

8dbeA Human prps1 with adp; hexamer (see paper)
48% identity, 97% coverage: 11:324/324 of query aligns to 1:314/316 of 8dbeA

query
sites
8dbeA

P

P

D

N

I

I

K

K

L

I

F

F

A

S

G

G

N

S

A

S

T

H

P

Q

S

D

L

L

A

S

K

Q

K

K

I

I

A

A

D

D

R

R

L

L

F

G

C

L

K

E

L

L

G

G

D

K

A

V

E

V

V

T

G

K

V

K

F
|
F

S

S

D
x
N

G

Q

E
|
E

I

T

S

C

V

V

Q

E

I

I

N

G

E

E

N

S

V

V

R

R

G

G

A

E

D

D

V

V

F

Y

I

I

I

V

Q

Q

S

S

T

G

C

C

A

G

P

E

T

I

N

N

D

D

N

N

L

L

M

M

E

E

L

L

I

L

V

I

M

M

V

I

D

N

A

A

L

C

R

K

R

I

A

A

S

S

A

A

G

S

R

R

I

V

T

T

A

A

V

V

I

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
x
K

V
x
D

R

K

S
|
S

A

-

R
|
R

V

A

P

P

I

I

T

S

A

A

K

K

V

L

V

V

A

A

D

N

F

M

L

L

S

S

S

V

V

A

G

G

V

A

D

D

R

H

V

I

L

I

T

T

C

M

D

D

L

L

H
|
H

A

A

E

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

V

I

P

P

V

V

D
|
D

N

N

V

L

F
x
Y

G

A

S

E

P

P

V

A

L

V

L

L

E

K

D

W

M

I

L

R

A

E

K

N

-

I

-

S

Q

E

L

W

D

R

N

N

P

C

V

T

V

I

V

V

S

S

P

P

D
|
D

I

A

G

G

V

A

V

K

R

R

A

V

R

T

A

S

V

I

A

A

K

D

L

R

L

L

D

N

D

-

S

V

D

D

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

R

P

K

Q

K

A

A

N

N

V

E

A

V

Q

D

V

R

M

M

H

V

I

L

I

V

G

G

D

D

V

V

Q

K

G

D

R

R

D

V

C

A

I

I

I

L

V

V

D
|
D

M

M

I

A

D
|
D

T
|
T

G

C

G
|
G

T
|
T

L

I

C

C

K

H

A

A

E

D

A

K

L

L

K

L

E

S

H

A

G

G

A

A

N

T

R

R

V

V

F

Y

A

A

Y

I

A

L

T

T

H

H

P

G

V

I

F

F

S

S

G

G

N

P

A

A

P

I

K

S

N

R

I

I

A

N

E

N

S

A

V

C

I

F

D

E

E

A

V

V

I

V

V

V

T

T

D

N

T

T

I

I

P

P

L

Q

S

E

E

D

E

K

M

M

A

K

A

H

L

C

D

S

K

K

V

I

T

Q

Q

V

L

I

T

D

M

I

S

S

S

M

V

I

M

L

A

A

E

E

A

A

I

I

R

R

V

T

S

H

N

N

E

G

E

E

S
|
S

I
x
V

S
|
S

A

Y

M

L

F
|
F

K

S

H

H

binding adenosine-5'-diphosphate: F34 (= F44), N36 (≠ D46), E38 (= E48), R95 (= R105), Q96 (= Q106), K98 (≠ R108), K99 (≠ R109), D100 (≠ V110), S102 (= S112), R103 (= R114), H129 (= H140), D142 (= D153), Y145 (≠ F156), S307 (= S317), V308 (≠ I318), S309 (= S319), F312 (= F322)
binding 5-O-phosphono-alpha-D-ribofuranose: H129 (= H140), D170 (= D179), D219 (= D229), D220 (= D230), D223 (= D233), T224 (= T234), G226 (= G236), T227 (= T237)

O94413 Ribose-phosphate pyrophosphokinase 2; Phosphoribosyl pyrophosphate synthase 2; EC 2.7.6.1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
49% identity, 96% coverage: 13:322/324 of query aligns to 6:316/321 of O94413

query
sites
O94413

I

I

K

K

L

I

F

F

A

A

G

G

N

N

A

S

T

H

P

P

S

E

L

L

A

A

K

E

K

K

I

V

A

A

D

R

R

R

L

I

F

G

C

L

K

S

L

L

G

G

D

K

A

V

E

A

V

V

G

V

V

Q

F

Y

S

S

D

N

G

R

E

E

I

T

S

S

V

V

Q

T

I

I

N

G

E

E

N

S

V

V

R

R

G

D

A

E

D

D

V

V

F

F

I

I

I

L

Q

Q

S

T

T

G

C

C

A

G

P

S

T

I

N

N

D

D

N

H

L

L

M

M

E

E

L

L

I

L

V

I

M

M

V

I

D

N

A

A

L

C

R

R

R

S

A

A

S

S

A

A

G

R

R

R

I

I

T

T

A

A

V

I

I

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R

R

Q

Q

D

D

R

K

V

D

R

K

S

S

A

-

R

R

V

A

P

P

I

I

T

T

A

A

K

R

V

L

V

V

A

A

D

N

F

M

L

L

S

Q

S

T

V

A

G

G

V

C

D

N

R

H

V

I

L

I

T

T

C

M

D

D

L

L

H

H

A

A

E

S

Q

Q

I

I

Q

Q

G

G

F

F

D

N

V

V

P

P

V

V

D

D

N

N

V

L

F

Y

G

A

S

E

P

P

V

S

L

V

L

L

E

R

D

-

M

Y

L

I

A

R

K

E

Q

N

L

I

D

D

-

T

-

V

N

N

P

P

-

T

V

V

V

I

V

V

S
|
S

P

P

D

D

I

A

G

G

V

A

V

K

R

R

A

A

R

T

A

A

V

L

A

A

K

D

L

R

L

L

D

D

D

-

S

L

D

D

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

R

P

Q

Q

K

A

A

N

N

V

E

A

V

Q

S

V

R

M

M

H

V

I

L

I

V

G

G

D

D

V

V

Q

R

G

D

R

K

D

L

C

A

I

I

I

L

V

V

D

D

M

M

I

A

D

D

T

T

G

C

G

G

T

T

L

L

C

G

K

L

A

A

E

K

A

T

L

L

K

K

E

D

H

N

G

G

A

A

N

K

R

A

V

V

F

Y

A

A

Y

I

A

V

T

T

H

H

P

G

V

I

F

L

S

S

G

G

N

K

A

A

P

I

K

K

N

V

I

I

A

N

E

E

S

S

V

A

I

L

D

E

E

K

V

V

I

I

V

V

T

T

D

N

T

T

I

I

P

P

L

H

S

D

E

D

E

K

M

R

A

S

A

L

L

C

D

S

K

K

V

I

T

E

Q

T

L

I

T

D

M

I

S

S

S

G

V

V

M

L

A

A

E

E

A

C

I

I

R

R

V

I

S

H

N

H

E

G

E

E

S

S

I

V

S

S

A

V

M

L

F

F

S172 (= S177) modified: Phosphoserine

8dbgA Human prps1 with phosphate and atp; hexamer (see paper)
47% identity, 97% coverage: 11:324/324 of query aligns to 1:307/309 of 8dbgA

query
sites
8dbgA

P

P

D

N

I

I

K

K

L

I

F

F

A

S

G

G

N

S

A

S

T

H

P

Q

S

D

L

L

A

S

K

Q

K

K

I

I

A

A

D

D

R

R

L

L

F

G

C

L

K

E

L

L

G

G

D

K

A

V

E

V

V

T

G

K

V

K

F
|
F

S

S

D
x
N

G

Q

E
|
E

I

T

S

C

V

V

Q

E

I

I

N

G

E

E

N
x
S

V

V

R
|
R

G

G

A

E

D

D

V

V

F

Y

I

I

I

V

Q

Q

S

S

T

G

C

C

A

G

P

E

T

I

N

N

D

D

N

N

L

L

M

M

E

E

L

L

I

L

V

I

M

M

V

I

D

N

A

A

L

C

R

K

R

I

A

A

S

S

A

A

G

S

R

R

I

V

T

T

A

A

V

V

I

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
x
K

R

K

V

D

R

K

S

S

A

-

R

R

V

A

P

P

I

I

T

S

A

A

K

K

V

L

V

V

A

A

D

N

F

M

L

L

S

S

S

V

V

A

G

G

V

A

D

D

R

H

V

I

L

I

T

T

C

M

D

D

L

L

H
|
H

A

A

E

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

V

I

P

P

V

V

D

D

N

N

V

L

F

Y

G

A

S

E

P

P

V

A

L

V

L

L

E

K

D

W

M

I

L

R

A

E

K

N

-

I

-

S

Q

E

L

W

D

R

N

N

P

C

V

T

V

I

V

V

S

S

P

P

D

D

I

A

G

G

V

A

V

K

R

R

A

V

R

T

A

S

V

I

A

A

K

D

L

R

L

L

D

-

D

N

S

V

D

D

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

-

P

-

Q

-

A

-

N

-

V

-

A

-

Q

D

V

R

M

M

H

V

I

L

I

V

G

G

D

D

V

V

Q

K

G

D

R

R

D

V

C

A

I

I

I

L

V

V

D

D

M

M

I

A

D
|
D

T
|
T

G
x
C

G

G

T
|
T

L

I

C

C

K

H

A

A

E

D

A

K

L

L

K

L

E

S

H

A

G

G

A

A

N

T

R

R

V

V

F

Y

A

A

Y

I

A

L

T

T

H

H

P

G

V

I

F

F

S

S

G

G

N

P

A

A

P

I

K

S

N

R

I

I

A

N

E

N

S

A

V

C

I

F

D

E

E

A

V

V

I

V

V

V

T

T

D

N

T

T

I

I

P

P

L

Q

S

E

E

D

E

K

M

M

A

K

A

H

L

C

D

S

K

K

V

I

T

Q

Q

V

L

I

T

D

M

I

S

S

S

M

V

I

M

L

A

A

E

E

A

A

I

I

R

R

V

T

S

H

N

N

E

G

E

E

S

S

I

V

S

S

A

Y

M

L

F

F

K

S

H

H

binding adenosine-5'-triphosphate: F34 (= F44), N36 (≠ D46), E38 (= E48), R95 (= R105), Q96 (= Q106), K98 (≠ R108), H129 (= H140)
binding phosphate ion: S46 (≠ N56), R48 (= R58), D216 (= D233), T217 (= T234), C218 (≠ G235), T220 (= T237)

2hcrA Crystal structure of human phosphoribosyl pyrophosphate synthetase 1 in complex with amp(atp), cadmium and sulfate ion (see paper)
47% identity, 96% coverage: 12:322/324 of query aligns to 1:305/305 of 2hcrA

query
sites
2hcrA

D

N

I

I

K

K

L

I

F

F

A

S

G

G

N

S

A

S

T

H

P

Q

S

D

L

L

A

S

K

Q

K

K

I

I

A

A

D

D

R

R

L

L

F

G

C

L

K

E

L

L

G

G

D

K

A

V

E

V

V

T

G

K

V

K

F

F

S

S

D

N

G

Q

E

E

I

T

S

C

V

V

Q

E

I

I

N

G

E

E

N

S

V

V

R

R

G

G

A

E

D

D

V

V

F

Y

I

I

I

V

Q

Q

S

S

T

G

C

C

A

G

P

E

T

I

N

N

D

D

N

N

L

L

M

M

E

E

L

L

I

L

V

I

M

M

V

I

D

N

A

A

L

C

R

K

R

I

A

A

S

S

A

A

G

S

R

R

I

V

T

T

A

A

V

V

I

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
x
K

R

K

V

D

R

K

S

S

A

-

R

R

V

A

P

P

I

I

T

S

A

A

K

K

V

L

V

V

A

A

D

N

F

M

L

L

S

S

S

V

V

A

G

G

V

A

D

D

R

H

V

I

L

I

T

T

C

M

D

D

L

L

H
|
H

A

A

E

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

V

I

P

P

V

V

D

D

N

N

V

L

F

Y

G

A

S

E

P

P

V

A

L

V

L

L

E

K

D

W

M

I

L

R

A

E

K

N

-

I

-

S

Q

E

L

W

D

R

N

N

P

C

V

T

V

I

V

V

S

S

P

P

D

D

I

A

G

G

V

A

V

K

R

R

A

V

R

T

A

S

V

I

A

A

K

D

L

R

L

L

D

-

D

N

S

V

D

D

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

R

P

D

Q

R

A

-

N

-

V

-

A

-

Q

-

V

-

M

M

H

V

I

L

I

V

G

G

D

D

V

V

Q

K

G

D

R

R

D

V

C

A

I

I

I

L

V

V

D

D

M

M

I

A

D

D

T

T

G

C

G

G

T

T

L

I

C

C

K

H

A

A

E

D

A

K

L

L

K

L

E

S

H

A

G

G

A

A

N

T

R

R

V

V

F

Y

A

A

Y

I

A

L

T

T

H

H

P

G

V

I

F

F

S

S

G

G

N

P

A

A

P

I

K

S

N

R

I

I

A

N

E

N

S

A

V

C

I

F

D

E

E

A

V

V

I

V

V

V

T

T

D

N

T

T

I

I

P

P

L

Q

S

E

E

D

E

K

M

M

A

K

A

H

L

C

D

S

K

K

V

I

T

Q

Q

V

L

I

T

D

M

I

S

S

S

M

V

I

M

L

A

A

E

E

A

A

I

I

R

R

V

T

S

H

N

N

E

G

E

E

S

S

I

V

S

S

A

Y

M

L

F

F

binding adenosine monophosphate: R94 (= R105), Q95 (= Q106), K97 (≠ R108), H128 (= H140)

7yk1A Structural basis of human prps2 filaments (see paper)
46% identity, 97% coverage: 11:324/324 of query aligns to 1:305/306 of 7yk1A

query
sites
7yk1A

P

P

D

N

I

I

K

V

L

L

F

F

A

S

G

G

N

S

A

S

T

H

P

Q

S

D

L

L

A

S

K

Q

K

R

I

V

A

A

D

D

R

R

L

L

F

G

C

L

K

E

L

L

G

G

D

K

A

V

E

V

V

T

G

K

V

K

F
|
F

S

S

D
x
N

G

Q

E
|
E

I

T

S

S

V

V

Q

E

I

I

N

G

E

E

N
x
S

V

V

R
|
R

G

G

A

E

D

D

V

V

F

Y

I

I

Q

Q

S

S

T

G

C

C

A

G

P

E

T

I

N

N

D

D

N

N

L

L

M

M

E

E

L

L

I

L

V

I

M

M

V

I

D

N

A

A

L

C

R

K

R

I

A

A

S

S

A

S

G

S

R

R

I

V

T

T

A

A

V

V

I

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R
|
R

Q

Q

D

D

R

K

R
x
K

V
x
D

R
x
K

S
|
S

A

-

R
|
R

V

A

P

P

I

I

T

S

A

A

K

K

V

L

V

V

A

A

D

N

F

M

L

L

S

S

S

V

V

A

G

G

V

A

D

D

R

H

V

I

L

I

T

T

C

M

D

D

L

L

H
|
H

A

A

E

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

V

I

P

P

V

V

D
|
D

N

N

V

L

F

Y

G

A

S

E

P

P

V

A

L

V

L

L

E

Q

D

W

M

I

L

R

A

E

K

N

-

I

-

A

Q

E

L

W

D

K

N

N

P

C

V

I

V

V

S

S

P

P

D

D

I

A

G

G

V

A

V

K

R

R

A

V

R

T

A

S

V

I

A

A

K

D

L

R

L

L

D

-

D

N

S

V

D

E

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

-

P

-

Q

-

A

-

N

-

V

-

A

-

Q

-

V

-

M

M

H

V

I

L

I

V

G

G

D

D

V

V

Q

K

G

D

R

R

D

V

C

A

I

I

I

L

V

V

D

D

M

M

I

A

D
|
D

T

T

G
x
C

G

G

T
|
T

L

I

C

C

K

H

A

A

E

D

A

K

L

L

K

L

E

S

H

A

G

G

A

A

N

T

R

K

V

V

F

Y

A

A

Y

I

A

L

T

T

H

H

P

G

V

I

F

F

S

S

G

G

N

P

A

A

P

I

K

S

N

R

I

I

A

N

E

N

S

A

V

A

I

F

D

E

E

A

V

V

I

V

V

V

T

T

D

N

T

T

I

I

P

P

L

Q

S

E

E

D

E

K

M

M

A

K

A

H

L

C

D

T

K

K

V

I

T

Q

Q

V

L

I

T

D

M

I

S

S

S

M

V

I

M

L

A

A

E

E

A

A

I

I

R

R

V

T

S

H

N

N

E

G

E

E

S
|
S

I

V

S
|
S

A

Y

M

L

F
|
F

K

S

H

H

binding adenosine-5'-diphosphate: F34 (= F44), N36 (≠ D46), E38 (= E48), S46 (≠ N56), R48 (= R58), R95 (= R105), K99 (≠ R109), D100 (≠ V110), K101 (≠ R111), S102 (= S112), R103 (= R114), H129 (= H140), D142 (= D153), S298 (= S317), S300 (= S319), F303 (= F322)
binding phosphate ion: D214 (= D233), C216 (≠ G235), T218 (= T237)

Query Sequence

>5210475 FitnessBrowser__PV4:5210475
MKHTPEVHTVPDIKLFAGNATPSLAKKIADRLFCKLGDAEVGVFSDGEISVQINENVRGA
DVFIIQSTCAPTNDNLMELIVMVDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAK
VVADFLSSVGVDRVLTCDLHAEQIQGFFDVPVDNVFGSPVLLEDMLAKQLDNPVVVSPDI
GGVVRARAVAKLLDDSDLAIIDKRRPQANVAQVMHIIGDVQGRDCIIVDDMIDTGGTLCK
AAEALKEHGANRVFAYATHPVFSGNAPKNIAESVIDEVIVTDTIPLSEEMAALDKVTQLT
MSSVMAEAIRRVSNEESISAMFKH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory