SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 5210577 FitnessBrowser__PV4:5210577 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

5odcC Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
29% identity, 19% coverage: 302:391/462 of query aligns to 25:110/184 of 5odcC

query
sites
5odcC

D

D

K

D

I

V

L

V

A

S

E

S

S

L

L

K

K

A

C
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C

I
x
Y

R
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Q

C
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C

G
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G

G

T

C
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C

L

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N

G

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C
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C

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G

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R

R

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S

G
x
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Y

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N

K

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L

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G

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M

A

D

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V

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Q

D

A

S

D

D

D

D

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I

H

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S

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-

W

W

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C

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T

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T

C
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C

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C
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C

S

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Y

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C

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D

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H

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R

N

L

L

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A

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M

binding iron/sulfur cluster: C34 (= C311), Y35 (≠ I312), Q36 (≠ R313), C37 (= C314), G38 (= G315), C40 (= C317), C44 (= C321), P45 (= P322), Y52 (≠ G329), T54 (≠ S331), C77 (= C358), T78 (= T359), T79 (≠ L360), C80 (= C361), Y81 (≠ G362), C83 (= C364), C87 (= C368), P88 (= P369), V91 (= V372)

8ahxC Cryo-em structure of the nitrogen-fixation associated nadh:ferredoxin oxidoreductase rnf from azotobacter vinelandii
35% identity, 17% coverage: 311:387/462 of query aligns to 368:433/476 of 8ahxC

query
sites
8ahxC

C
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C

I
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I

R
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R

C
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C

G
x
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G
x
S

C
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C

L

V

N

D

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C
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C

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M

Y

-

R

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G

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G

G

Y

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Y

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N

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Y

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F

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binding flavin mononucleotide: C410 (= C361)
binding iron/sulfur cluster: C368 (= C311), I369 (= I312), R370 (= R313), C371 (= C314), A372 (≠ G315), S373 (≠ G316), C374 (= C317), C378 (= C321), P379 (= P322), C407 (= C358), I408 (≠ T359), L409 (= L360), C410 (= C361), G411 (= G362), C413 (= C364), C417 (= C368), P418 (= P369), S419 (≠ T370)

Sites not aligning to the query:

binding flavin mononucleotide: 137, 139, 163, 167, 238, 239, 266, 267, 334

5odcI Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
29% identity, 19% coverage: 302:391/462 of query aligns to 25:110/173 of 5odcI

query
sites
5odcI

D

D

K

D

I

V

L

V

A

S

E

S

S

L

L

K

K

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C
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C

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C

G
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G

G

T

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C

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T

N

G

T

S

C
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C

P
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P

V

S

Y

G

R

R

R

R

S

T

G

S

G
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Y
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S
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K

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K

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P

L

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L

G

G

I

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A

D

V

D

G

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L

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A

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D

D

D
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D

T

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H

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S

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I

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P

-

W

W

A

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C

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C

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C

S

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Y

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C

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A

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L

M

binding magnesium ion: R53 (≠ Y330), D73 (= D350)
binding iron/sulfur cluster: C34 (= C311), Y35 (≠ I312), Q36 (≠ R313), C37 (= C314), G38 (= G315), C40 (= C317), C44 (= C321), P45 (= P322), Y52 (≠ G329), T54 (≠ S331), C77 (= C358), T78 (= T359), T79 (≠ L360), C80 (= C361), Y81 (≠ G362), C83 (= C364), C87 (= C368), V91 (= V372)

Query Sequence

>5210577 FitnessBrowser__PV4:5210577
MSSQTTALNSGVHAKKADIFCQDEARVDWHSKALWVLREKRDRAAASLPEWEQLRQLGSE
MKLHTLTHLGEYLETFEKNCQANGIVVHWAKDGAEHNQIVHNILAKHQVKKLVKSKSMLT
EECHLNPYLESKGIEVIDTDLGERIIQLAKQPPSHIVVPAIHLKKEEVGDLFHDKLGTEA
GASDPLYLTRAARAHLREQFLSADAAMTGVNMAIADKGAVVVCTNEGNADMGANLPKLQL
HSMGIDKIVPDLDSAAILLRTLARNATGQPITTYSSFYRGPQDGGEMHVIIVDNGRTDML
QDKILAESLKCIRCGGCLNTCPVYRRSGGYSYNYTIPGPIGIAVGAQADDTHSIPWACTL
CGSCSYVCPTKVPLDKIIHHHRRLKAKAGKLPYGKRNYMPLVGNFMASETMLNCSMSVAR
TALRILPGSLLKPFSGAWGKYRELPVAPKSSFEAWFNKNRGQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory