SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 56 a.a. (MERAFQNRCE...) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

O34800 Stage II sporulation protein SB; Antidote protein SpoIISB; Antitoxin SpoIISB from Bacillus subtilis (strain 168) (see paper)
100% identity, 100% coverage: 1:56/56 of query aligns to 1:56/56 of O34800

query
sites
O34800

M
|
M

E
|
E

R
|
R

A
|
A

F
|
F

Q
|
Q

N
|
N

R
|
R

C
|
C

E
|
E

P
|
P

R
|
R

A

A

A

A

K

K

P

P

F

F

K

K

I

I

L

L

K

K

K

K

R

R

S

S

T

T

T

T

S

S

V

V

A

A

S

S

Y

Y

Q

Q

V

V

S

S

P

P

H

H

T

T

A

A

R

R

I

I

F

F

K

K

E

E

N

N

E

E

R

R

L

L

I

I

D

D

E

E

Y

Y

K

K

R
|
R

K
|
K

K
|
K

A
|
A

1:12 (vs. 1:12, 100% identical) mutation to MA: No loss of antitoxin activity.
RKKA 53:56 (= RKKA 53:56) mutation Missing: Significant loss of antitoxin activity.

Query Sequence

>56 a.a. (MERAFQNRCE...)
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory