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SitesBLAST – Find functional sites

SitesBLAST

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Comparing 58 a.a. (ERKRMRNRIA...) to proteins with known functional sites using BLASTp with E ≤ 0.001.

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Found 11 hits to proteins with known functional sites (download)

P05412 Transcription factor Jun; Activator protein 1; AP1; Proto-oncogene c-Jun; Transcription factor AP-1 subunit Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39 from Homo sapiens (Human) (see 12 papers)
98% identity, 100% coverage: 1:58/58 of query aligns to 256:313/331 of P05412

query
sites
P05412

E

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K271 (= K16) modified: N6-acetyllysine
R272 (= R17) mutation to V: Abolishes the synergistic activity with SMAD3 to activate TGF-beta-mediated transcription.
T286 (= T31) modified: Phosphothreonine; by PAK2; mutation to A: Complete loss of PAK2-mediated phosphorylation; when associated with A-2; A-8; A-89; and A-93.

Sites not aligning to the query:

2 modified: Phosphothreonine; by PAK2; T→A: Complete loss of PAK2-mediated phosphorylation; when associated with A-8; A-89; A-93; and A-286.
6:194 mutation Missing: Abolishes activation of FASLG/CD95L transcription.
8 modified: Phosphothreonine; by PAK2; T→A: Complete loss of PAK2-mediated phosphorylation; when associated with A-2; A-89; A-93; and A-286.
63 modified: Phosphoserine; by MAPK8 and PLK3; S→A: Greatly reduced ATF7-mediated transcriptional activity; when associated with A-73. Abolishes interaction with FBXW7; when associated with A-73; A-91 and A-93.
73 modified: Phosphoserine; by MAPK8 and PLK3; S→A: Greatly reduced ATF7-mediated transcriptional activity; when associated with A-63. Abolishes interaction with FBXW7; when associated with A-63; A-91 and A-93.
89 modified: Phosphothreonine; by PAK2; T→A: Complete loss of PAK2-mediated phosphorylation; when associated with A-2; A-8; A-93; and A-286.
91 modified: Phosphothreonine; T→A: Abolishes interaction with FBXW7; when associated with A-63; A-73 and A-93.
93 modified: Phosphothreonine; by PAK2; T→A: Abolishes interaction with FBXW7; when associated with A-63; A-73 and A-91.; T→A: Complete loss of PAK2-mediated phosphorylation; when associated with A-2; A-8; A-89; and A-286.
150:223 Interaction with PAGE4
239 modified: Phosphothreonine; by GSK3-beta
243 modified: Phosphoserine; by DYRK2 and GSK3-beta; S→A: Abolishes phosphorylation by DYRK2. Abolishes phosphorylation by GSK3B at Thr-239.
249 modified: Phosphoserine; by GSK3-beta

5t01A Human c-jun DNA binding domain homodimer in complex with methylated DNA (see paper)
100% identity, 100% coverage: 1:58/58 of query aligns to 5:62/62 of 5t01A

query
sites
5t01A

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binding : R8 (= R4), R12 (= R8), R19 (= R15)

Sites not aligning to the query:

binding : 1, 2

P17535 Transcription factor JunD; Transcription factor AP-1 subunit JunD from Homo sapiens (Human) (see 2 papers)
86% identity, 100% coverage: 1:58/58 of query aligns to 272:329/347 of P17535

query
sites
P17535

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C285 (≠ S14) modified: Interchain (with C-172 in FOSB)

Sites not aligning to the query:

20 G → V: in dbSNP:rs41478151
27:39 Menin-binding motif (MBM)
30 R→A: Reduced interaction with MEN1.
31 L→A: Reduced interaction with MEN1.
32 F→A: Loss of interaction with MEN1.
33 P→A: Loss of interaction with MEN1.
34 G→R: Loss of interaction with MEN1.
35 A→R: Reduced interaction with MEN1.
36 P→A: Reduced interaction with MEN1.
37 P→A: Reduced interaction with MEN1.
46 K→A: Loss of phosphorylation; when associated with A-47.; K→E: Reduced interaction with MEN1; when associated with E-47.
46:55 MAP kinase docking motif; essential for its phosphorylation
47 K→A: Loss of phosphorylation; when associated with A-46.; K→E: Reduced interaction with MEN1; when associated with E-46.
52 L→A: Loss of phosphorylation; when associated with A-54.
54 L→A: Loss of phosphorylation; when associated with A-52.
90 modified: Phosphoserine
100 modified: Phosphoserine; by MAPK8
117 modified: Phosphothreonine

5vpeD Transcription factor fosb/jund bzip domain in complex with cognate DNA, type-i crystal (see paper)
86% identity, 100% coverage: 1:58/58 of query aligns to 8:65/67 of 5vpeD

query
sites
5vpeD

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binding : R11 (= R4), R13 (= R6), N14 (= N7), K20 (= K13), C21 (≠ S14), R22 (= R15), R24 (= R17), K25 (= K18)

P18289 Transcription factor Jra; Jun-related antigen; Transcription factor AP-1 subunit Jra; dJRA; dJun from Drosophila melanogaster (Fruit fly) (see paper)
66% identity, 100% coverage: 1:58/58 of query aligns to 216:273/289 of P18289

query
sites
P18289

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Sites not aligning to the query:

82 modified: Phosphoserine

5fv8E Structure of cjun-fosw coiled coil complex.
97% identity, 55% coverage: 22:53/58 of query aligns to 3:34/37 of 5fv8E

query
sites
5fv8E

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binding o-(o-(2-aminopropyl)-o'-(2-methoxyethyl)polypropylene glycol 500): N25 (= N44), E29 (= E48)

5fv8D Structure of cjun-fosw coiled coil complex.
97% identity, 52% coverage: 22:51/58 of query aligns to 3:32/32 of 5fv8D

query
sites
5fv8D

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binding o-(o-(2-aminopropyl)-o'-(2-methoxyethyl)polypropylene glycol 500): A15 (= A34), Y18 (≠ S37)

P17544 Cyclic AMP-dependent transcription factor ATF-7; cAMP-dependent transcription factor ATF-7; Activating transcription factor 7; Transcription factor ATF-A from Homo sapiens (Human) (see 5 papers)
51% identity, 86% coverage: 6:55/58 of query aligns to 341:390/483 of P17544

query
sites
P17544

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Sites not aligning to the query:

9 C→A: Severely reduced TAF12-mediated enhancement of transcriptional activity.
14 C→A: Greatly reduced JNK2- or adenovirus E1A-mediated transactivation. Abolishes adenovirus 2 E1A-mediated transactivation; when associated with A-51; A-53 and G-101.
22 D→K: No effect on binding adenovirus 2 E1A. Abolishes ATF7-mediated E1A responsiveness.
27 H→N: No effect on binding adenovirus 2 E1A. Abolishes ATF7-mediated E1A responsiveness.
33 M→D: Severely reduced TAF12-induced transcriptional activity; when associated with S-35.
35 L→S: Severely reduced TAF12-induced transcriptional activity; when associated with D-33.
51 modified: Phosphothreonine; by MAPK11; T→A: Severely reduced TAF12-induced transcriptional activity. No effect on MAPK9-mediated phosphorylation; when associated with A-53. Greatly reduces MAPK9- and adenovirus E1A-mediated transactivation; when associated with A-53 and G-101. Abolishes adenovirus 2 E1A-mediated transactivation; when associated with A-14; A-53 and G-101.; T→D: Completely abolishes MAPK9- and adenovirus E1A-mediated transactivation; when associated with D-53.
53 modified: Phosphothreonine; T→A: Severely reduced TAF12-induced transcriptional activity. No effect on MAPK9-mediated phosphorylation; when associated with A-51. Greatly reduces MAPK9- and adenovirus E1A-mediated transactivation; when associated with A-51 and G-101. Abolishes adenovirus 2 E1A-mediated transactivation; when associated with A-14; A-51 and G-101.; T→D: Completely abolishes MAPK9- and adenovirus E1A-mediated transactivation; when associated with D-51.
101 modified: Phosphothreonine; T→G: Some reduction in transactivation but, completely abolishes MAPK9-mediated activation. Abolishes MAPK9-mediated and greatly reduces adenovirus E1A-mediated transactivation; when associated with A-51 and A-53. Abolishes adenovirus 2 E1A-mediated transactivation; when associated with A-14; A-51 and A-53.
107 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); K→R: Abolishes sumoylation. Exclusive nucleoplasmic location. Increase in binding the E-selectin promoter.
145 T→A: No effect on transactivation; when associated with A-147.
147 T→A: No effect on transactivation; when associated with A-145.

1t2kD Structure of the DNA binding domains of irf3, atf-2 and jun bound to DNA (see paper)
43% identity, 91% coverage: 6:58/58 of query aligns to 8:60/61 of 1t2kD

query
sites
1t2kD

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binding : R8 (= R6), N9 (= N7), R10 (= R8), R15 (≠ K13), S16 (= S14), R17 (= R15), K19 (≠ R17)

Sites not aligning to the query:

binding : 2

Q96BA8 Cyclic AMP-responsive element-binding protein 3-like protein 1; cAMP-responsive element-binding protein 3-like protein 1; Old astrocyte specifically-induced substance; OASIS from Homo sapiens (Human) (see 2 papers)
42% identity, 66% coverage: 2:39/58 of query aligns to 295:332/519 of Q96BA8

query
sites
Q96BA8

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Sites not aligning to the query:

392 P→A: Abolishes proteolytic cleavage by MBTPS2; when associated with A-395.; P→L: Abolishes proteolytic cleavage by MBTPS2.
395 P→A: Abolishes proteolytic cleavage by MBTPS2; when associated with A-392.
423 R→A: Abolishes proteolytic cleavage by MBTPS1.
426 L→V: Abolishes proteolytic cleavage by MBTPS1.

Q9BYV9 Transcription regulator protein BACH2; BTB and CNC homolog 2 from Homo sapiens (Human) (see 3 papers)
40% identity, 90% coverage: 2:53/58 of query aligns to 651:702/841 of Q9BYV9

query
sites
Q9BYV9

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Sites not aligning to the query:

20 modified: Interchain; redox-active
24 L → P: in IMD60; impairs protein stability; the mutant is unsoluble and forms multiple aggregates
521 modified: Phosphoserine; by RPS6KB1; S→A: Leads to nuclear accumulation.
788 E → K: in IMD60; uncertain significance; severely decreased localization in the nucleus; the mutant aggregates in the cytoplasm; dbSNP:rs757652995

Query Sequence

>58 a.a. (ERKRMRNRIA...)
ERKRMRNRIAASKSRKRKLERIARLEEKVKTLKAQNSELASTANMLREQVAQLKQKVM

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory