SitesBLAST

P29674 LIM/homeobox protein Lhx1; LIM homeobox protein 1; Homeobox protein Lim-1; Xlim1; x-Lhx1; xLIM-1 from Xenopus laevis (African clawed frog) (see 4 papers)
74% identity, 98% coverage: 2:58/58 of query aligns to 182:238/403 of P29674

query
sites
P29674

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I223 (≠ V43) mutation to P: Fails to induce chrd.

Sites not aligning to the query:

28 C→G: Disrupts ldb1-binding. Produces an active form; when associated with G-88.
88 C→G: Disrupts ldb1-binding. Produces an active form; when associated with G-28.
278 Y→A: Abolishes transcriptional activity and axis-inducing activity; when associated with A-281; A-285; A-286 and A-292.; Y→F: No effect on axis-inducing activity; when associated with F-281; F-285; F-286 and F-292.
281 Y→A: Abolishes transcriptional activity and axis-inducing activity; when associated with A-278; A-285; A-286 and A-292.; Y→F: No effect on axis-inducing activity; when associated with F-278; F-285; F-286 and F-292.
285 Y→A: Abolishes transcriptional activity and axis-inducing activity; when associated with A-278; A-281; A-286 and A-292.; Y→F: No effect on axis-inducing activity; when associated with F-278; F-281; F-286 and F-292.
286 Y→A: Abolishes transcriptional activity and axis-inducing activity; when associated with A-278; A-281; A-285 and A-292.; Y→F: No effect on axis-inducing activity; when associated with F-278; F-281; F-285 and F-292.
292 Y→A: Abolishes transcriptional activity and axis-inducing activity; when associated with A-278; A-281; A-285 and A-286.; Y→F: No effect on axis-inducing activity; when associated with F-278; F-281; F-285 and F-286.

P09088 Mechanosensory protein 3 from Caenorhabditis elegans (see 3 papers)
61% identity, 98% coverage: 2:58/58 of query aligns to 220:276/321 of P09088

query
sites
P09088

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Sites not aligning to the query:

69:321 mutation Missing: In e1338; homeotic transformation of ALM to BDU neurons, based on altered cell body and axonal morphology and ectopically expressed BDU markers in ALM, including abnormal expression of ceh-14, flp-10, nlp-1, ser-2 and zig-3. Abnormal ectopic expression of ALN markers lad-2 and cho-1 in tail neurons. PVD neurons have severe dendritic branching defects, and significantly longer axonal projection in the ventral nerve cord. On either egl-44 or egl-46 mutant backgrounds, abolishes expression of tyramine receptor ser-2. Abolishes expression of alr-1 in a specific set of touch receptor neurons.

Q21192 LIM/homeobox protein lim-6 from Caenorhabditis elegans (see paper)
61% identity, 98% coverage: 1:57/58 of query aligns to 188:244/316 of Q21192

query
sites
Q21192

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Sites not aligning to the query:

106 C→Y: In ot146; Impairs left/right asymmetric gene expression programs of two gustatory neurons, ASEL and ASER. In particular, markers of ASER cell fate, such as receptor-type guanylate cyclase gcy-5, are gained in ASEL, but ASEL fate markers are unaffected.

O60663 LIM homeobox transcription factor 1-beta; LIM/homeobox protein 1.2; LMX-1.2; LIM/homeobox protein LMX1B from Homo sapiens (Human) (see 6 papers)
58% identity, 98% coverage: 1:57/58 of query aligns to 221:277/402 of O60663

query
sites
O60663

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R246 (= R26) to Q: in FSGS10; decreased transcriptional activity; dbSNP:rs1191455921
V265 (= V45) mutation to L: Loss of transcriptional activity.

8ik5C Transcription factor lmx1a homeobox domain in complex with wnt1 promoter
58% identity, 98% coverage: 1:57/58 of query aligns to 8:64/67 of 8ik5C

query
sites
8ik5C

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binding : R10 (= R3), R10 (= R3), T11 (= T4), K30 (= K23), R33 (= R26), V52 (= V45), Q55 (= Q48), N56 (= N49), R58 (= R51), K60 (= K53)

Sites not aligning to the query:

binding : 7

Q61329 Zinc finger homeobox protein 3; AT motif-binding factor 1; AT-binding transcription factor 1; Alpha-fetoprotein enhancer-binding protein; Zinc finger homeodomain protein 3; ZFH-3 from Mus musculus (Mouse) (see 2 papers)
50% identity, 93% coverage: 3:56/58 of query aligns to 2654:2707/3726 of Q61329

query
sites
Q61329

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Sites not aligning to the query:

1600 modified: Phosphoserine
1963 V→F: In short circuit/sci mutant; shorter circadian period, homozygous lethality during embryonic development and diminished ability to activate the transcription of circadian-related neuropeptides.
2634 modified: Phosphoserine
2795 modified: Phosphoserine
2804 modified: Phosphoserine
2900 modified: Phosphoserine
3434 modified: Phosphoserine
3616 modified: Phosphoserine
3700 modified: Phosphoserine

Q15911 Zinc finger homeobox protein 3; AT motif-binding factor 1; AT-binding transcription factor 1; Alpha-fetoprotein enhancer-binding protein; Zinc finger homeodomain protein 3; ZFH-3 from Homo sapiens (Human) (see 4 papers)
50% identity, 93% coverage: 3:56/58 of query aligns to 2645:2698/3703 of Q15911

query
sites
Q15911

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Sites not aligning to the query:

72 S → A: in dbSNP:rs7193297
777 V → A: in dbSNP:rs4788682
997 A → S: in dbSNP:rs2213978
2349 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); K→A: Loss of sumoylation.
2615:2617 Nuclear localization signal; KRK→AAA: Loss of nuclear localization.
2806 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternate; K→A: Loss of sumoylation.
3258 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); K→A: Loss of sumoylation.
3527 natural variant: G -> GGG

Q2MHN3 Zinc finger homeobox protein 2; Zinc finger homeodomain protein 5 from Mus musculus (Mouse) (see paper)
50% identity, 97% coverage: 1:56/58 of query aligns to 1853:1908/2562 of Q2MHN3

query
sites
Q2MHN3

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R1907 (≠ K55) mutation to K: Results in hyposensitivity to noxious heat.

P28167 Zinc finger protein 2; Zinc finger homeodomain protein 2 from Drosophila melanogaster (Fruit fly) (see paper)
48% identity, 97% coverage: 1:56/58 of query aligns to 2762:2817/3005 of P28167

query
sites
P28167

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Sites not aligning to the query:

1337 modified: Phosphothreonine
2361 modified: Phosphoserine
2450 modified: Phosphoserine
2451 modified: Phosphoserine
2749 modified: Phosphoserine
2751 modified: Phosphoserine

P09083 Protein gooseberry-neuro; BSH4; Protein gooseberry proximal from Drosophila melanogaster (Fruit fly) (see paper)
45% identity, 97% coverage: 1:56/58 of query aligns to 184:239/449 of P09083

query
sites
P09083

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Sites not aligning to the query:

165 modified: Phosphoserine

8osbE Twist1-tcf4-alx4 complex on specific DNA (see paper)
51% identity, 91% coverage: 1:53/58 of query aligns to 1:53/62 of 8osbE

query
sites
8osbE

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binding : R1 (= R1), N2 (≠ P2), R3 (= R3), R3 (= R3), T4 (= T4), T5 (= T5), F6 (≠ I6), Y23 (≠ K23), R42 (≠ V42), Q44 (= Q44), Q48 (= Q48), N49 (= N49), R51 (= R51)

Sites not aligning to the query:

binding : 55

Q8IRC7 LIM/homeobox protein Awh; Protein arrowhead from Drosophila melanogaster (Fruit fly) (see 2 papers)
45% identity, 97% coverage: 1:56/58 of query aligns to 150:205/275 of Q8IRC7

query
sites
Q8IRC7

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Sites not aligning to the query:

57 C→Y: In allele 11; may cause loss of zinc binding.
88 L→T: In allele 13; may alter structure of LIM domain.
117 V→E: In allele 17; may alter structure of LIM domain.
126 modified: Phosphothreonine

G5ED66 Paired box protein 3 homolog from Caenorhabditis elegans (see paper)
43% identity, 97% coverage: 1:56/58 of query aligns to 189:244/308 of G5ED66

query
sites
G5ED66

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Sites not aligning to the query:

29 P→L: In ga96; causes defects in embryonic development, larval body morphology and vulval development. Adult hermaphrodites have gonad migration defects in which one or both arms of the developing gonad fail to turn correctly. Decrease in the number of ventral hypodermal cells (Pn.p cells) and in the number of ventral cord neurons (Pn.a cells). Ectopic expression of scm in P cells in 27% of animals raised at the restrictive temperature, 25 degrees Celsius.

L8E946 Homeobox protein unc-42; Uncoordinated protein 42 from Caenorhabditis elegans (see 5 papers)
48% identity, 97% coverage: 1:56/58 of query aligns to 102:157/279 of L8E946

query
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L8E946

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V146 (= V45) mutation to E: In e270; axon outgrowth, pioneering and pathfinding defects; mechanosensory defects.

Sites not aligning to the query:

107:279 mutation Missing: In e419; axon outgrowth and pathfinding defects; mechanosensory defects; abolishes expression of oligopeptide transporter pept-3 in AVE neurons, and both FMRFamide related neuropeptide precursor gene flp-1 and neuron specific calcium sensor ncs-1 in AVK neurons. Abolishes expression of seven-transmembrane receptor srb in ASH neurons. Chemical synaptic connectivity is reduced in neurons that normally express unc-42 in the pre- and post-synaptic neurons. Reduces expression of vesicular acetylcholine transporter unc-17 and hence cholinergic identity in all but one of the normally unc-42(+) neurons. Reduces the expression of cholinergic receptor systems, and also the expression of the tyramine-gated chloride channel lgc-55. Highly patterned expression of neuropeptides and neuropeptide receptors is severely affected. Expression of innexins inx-18a and inx-19 is selectively down-regulated in those neurons that normally express unc-42. Abolishes expression of putative osmosensor osm-10 in ASH neurons.
147:279 mutation Missing: In gm23; axon pioneering and pathfinding defects.

3a01F Crystal structure of aristaless and clawless homeodomains bound to dna (see paper)
49% identity, 91% coverage: 1:53/58 of query aligns to 2:54/61 of 3a01F

query
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3a01F

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L

L

S

A

S

M

E

K

T

I

G

G

L

L

D

T

M

E

R

A

V

R

V

I

Q

Q

V

V

W

W

F

F

Q
|
Q

N
|
N

R

R

R
|
R

A

A

K
|
K

binding : R2 (= R1), R2 (= R1), R4 (= R3), R4 (= R3), T5 (= T4), F7 (≠ I6), Y24 (≠ K23), V27 (≠ R26), Q49 (= Q48), N50 (= N49), R52 (= R51), K54 (= K53)

Sites not aligning to the query:

binding : 55, 56, 60

G5EC36 LIM/homeobox protein lim-7 from Caenorhabditis elegans (see 2 papers)
41% identity, 97% coverage: 1:56/58 of query aligns to 267:322/452 of G5EC36

query
sites
G5EC36

R

R

P

V

R

R

T

T

T

V

I

L

T

N

A

E

K

N

Q

Q

L

L

E

K

T

I

L

L

K

R

N

D

A

C

Y

Y

K

S

N

I

S

N

P

S

K

R

P

P

A

D

R

A

H

T

V

L

R

K

E

E

Q

R

L

L

S

V

S

E

E

M

T

T

G

G

L

L

D

S

M

A

R

R

V

V

V

I

Q

R

V

V

W

W

F

F

Q

Q

N

N

R

K

R

R

A

C

K

K

E

D

K

K

R

K

Sites not aligning to the query:

84:161 mutation Missing: In tm674; larval (L1 stage) lethality.
347:376 LIM interaction domain (LID)

Q8C8B0 ALX homeobox protein 1; Cartilage homeoprotein 1; CART-1 from Mus musculus (Mouse) (see paper)
53% identity, 91% coverage: 1:53/58 of query aligns to 134:186/326 of Q8C8B0

query
sites
Q8C8B0

R

R

P

H

R

R

T

T

I

F

T

T

A

S

K

L

Q

Q

L

L

E

E

T

E

L

L

K

E

N

K

A

V

Y

F

K

Q

N

K

S

T

P

H

K

Y

P

P

A

D

R

V

H

Y

V

V

R

R

E

E

Q

Q

L

L

S

A

S

L

E

R

T

T

G

E

L

L

D

T

M

E

R

A

V

R

V

V

Q

Q

V

V

W

W

F

F

Q

Q

N

N

R

R

A

A

K

K

Sites not aligning to the query:

131 modified: N6-acetyllysine; by EP300; K→R: Loss of acetylation. Decreased binding affinity to EP300. Loss of EP300-mediated transcriptional coactivation.

Query Sequence

>58 a.a. (RPRTTITAKQ...)
RPRTTITAKQLETLKNAYKNSPKPARHVREQLSSETGLDMRVVQVWFQNRRAKEKRLK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory