SitesBLAST

R173 (= R42) mutation to A: Reduces the ability of ALPHA2/MCM1 to repress a-specific genes. Disrupts interaction with SSN6.
S181 (= S50) mutation to A: In ALPHA2-3A; defective in binding DNA alone or in complex with MCM1, but binds DNA normally in complex with A1; when associated with A-182 and A-185.
N182 (= N51) mutation to A: In ALPHA2-3A; defective in binding DNA alone or in complex with MCM1, but binds DNA normally in complex with A1; when associated with A-181 and A-185.
R185 (= R54) mutation to A: In ALPHA2-3A; defective in binding DNA alone or in complex with MCM1, but binds DNA normally in complex with A1; when associated with A-181 and A-182.

Sites not aligning to the query:

1 modified: N-acetylmethionine
4 I→T: Reduces the ability of ALPHA2 to repress transcription, but binds normally to DNA and MCM1.
9 L→F: Reduces the ability of ALPHA2 to repress transcription, but binds normally to DNA and MCM1.
10 L→S: Reduces the ability of ALPHA2 to repress transcription, but binds normally to DNA and MCM1. Disrupts interaction with TUP1.
71 E→K: Reduces the ability of alpha2 to repress transcription, but binds normally to DNA and MCM1.
114 L→A: Reduces the ability of ALPHA2/MCM1 to repress a-specific genes.
115 V→A: Reduces the ability of ALPHA2/MCM1 to repress a-specific genes.
116 F→A: Reduces the ability of ALPHA2/MCM1 to repress a-specific genes.
117 N→A: Reduces the ability of ALPHA2/MCM1 to repress a-specific genes.
118 V→A: Reduces the ability of ALPHA2/MCM1 to repress a-specific genes.
119 V→A: Reduces the ability of ALPHA2/MCM1 to repress a-specific genes.
120 T→A: Reduces the ability of ALPHA2/MCM1 to repress a-specific genes.
192 I→A: Disrupts the ability of A1/ALPHA2 to repress haploid-specific genes.
196 mutation L->A,S: Disrupts the ability of A1/ALPHA2 to repress haploid-specific genes.
199 L→A: Disrupts the ability of A1/ALPHA2 to repress haploid-specific genes.
200 L→A: Disrupts the ability of A1/ALPHA2 to repress haploid-specific genes.

1akhB Mat a1/alpha2/DNA ternary complex (see paper)
100% identity, 100% coverage: 1:59/59 of query aligns to 5:63/78 of 1akhB

query
sites
1akhB

R
|
R

G
|
G

H

H

R
|
R

F
|
F

T

T

K

K

E

E

N

N

V

V

R

R

I

I

L

L

E

E

S

S

W

W

F

F

A

A

K

K

N

N

I

I

E

E

N

N

P

P

Y
|
Y

L

L

D

D

T

T

K

K

G

G

L

L

E

E

N

N

L

L

M

M

K

K

N

N

T

T

S

S

L

L

S

S

R

R

I

I

Q
|
Q

I

I

K
|
K

N

N

W

W

V

V

S

S

N
|
N

R

R

R
|
R

K
|
K

E

E

K
|
K

T

T

I

I

binding : R5 (= R1), R5 (= R1), G6 (= G2), G6 (= G2), R8 (= R4), R8 (= R4), F9 (= F5), Y29 (= Y25), Q48 (= Q44), K50 (= K46), N55 (= N51), R57 (= R53), R58 (= R54), K59 (= K55), K61 (= K57)

Sites not aligning to the query:

binding : 4, 4

1mnmC Yeast matalpha2/mcm1/DNA ternary transcription complex crystal structure (see paper)
100% identity, 98% coverage: 1:58/59 of query aligns to 20:77/77 of 1mnmC

query
sites
1mnmC

R
|
R

G
|
G

H
|
H

R
|
R

F
|
F

T

T

K

K

E

E

N

N

V

V

R

R

I

I

L

L

E

E

S

S

W

W

F

F

A

A

K

K

N

N

I

I

E

E

N

N

P

P

Y
|
Y

L

L

D

D

T

T

K

K

G

G

L

L

E

E

N

N

L

L

M

M

K

K

N

N

T

T

S

S

L

L

S

S

R

R

I

I

Q
|
Q

I

I

K
|
K

N

N

W
|
W

V

V

S

S

N
|
N

R

R

R
|
R

K
|
K

E

E

K

K

T

T

binding : R20 (= R1), R20 (= R1), G21 (= G2), H22 (= H3), R23 (= R4), R23 (= R4), F24 (= F5), Y44 (= Y25), Q63 (= Q44), K65 (= K46), W67 (= W48), N70 (= N51), R72 (= R53), R73 (= R54), K74 (= K55)

Sites not aligning to the query:

binding : 19

Query Sequence

>59 a.a. (RGHRFTKENV...)
RGHRFTKENVRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory