SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

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Comparing 62 a.a. (TTNADRRKAA...) to proteins with known functional sites using BLASTp with E ≤ 0.001.

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Found 9 hits to proteins with known functional sites (download)

1mdyA Crystal structure of myod bhlh domain bound to dna: perspectives on DNA recognition and implications for transcriptional activation (see paper)
100% identity, 100% coverage: 1:62/62 of query aligns to 7:68/68 of 1mdyA

query
sites
1mdyA

T

T

N

N

A

A

D

D

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A

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T

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D

D

binding : R13 (= R7), T17 (= T11), R19 (= R13), E20 (= E14), R21 (= R15), R23 (= R17), P47 (= P41), K48 (= K42)

P15172 Myoblast determination protein 1; Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3 from Homo sapiens (Human) (see 4 papers)
98% identity, 100% coverage: 1:62/62 of query aligns to 105:166/320 of P15172

query
sites
P15172

T

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Sites not aligning to the query:

1 modified: Peptide (Met-Gly) (interchain with G-Cter in ubiquitin)
104 modified: N6-methyllysine; by EHMT2
233:320 natural variant: Missing (in CMYO17; uncertain significance)
262 E → K: in a breast cancer sample; somatic mutation; dbSNP:rs930939009
309 A → V: in a breast cancer sample; somatic mutation; dbSNP:rs1471293207

7z5iA Transcription factor myf5 bound to symmetrical site
89% identity, 85% coverage: 5:57/62 of query aligns to 3:55/56 of 7z5iA

query
sites
7z5iA

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binding : R4 (= R6), R5 (= R7), T9 (= T11), R11 (= R13), E12 (= E14), R13 (= R15), R14 (= R16), R15 (= R17), P39 (= P41), K40 (= K42)

P20428 Myogenin from Rattus norvegicus (Rat) (see paper)
77% identity, 97% coverage: 1:60/62 of query aligns to 77:136/287 of P20428

query
sites
P20428

T
x
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S77 (≠ T1) modified: Phosphoserine; by CaMK2G; mutation to A: Reduces phosphorylation.
S79 (≠ N3) modified: Phosphoserine; by CaMK2G; mutation to A: Reduces phosphorylation.
T87 (= T11) modified: Phosphothreonine; by CaMK2G; mutation to A: Reduces phosphorylation and inhibits DNA-binding and transcriptional activation.

P22980 Myoblast determination protein 1 homolog; MyoD protein 1; Helix-loop-helix protein 1 from Caenorhabditis elegans (see paper)
74% identity, 92% coverage: 5:61/62 of query aligns to 155:211/324 of P22980

query
sites
P22980

D

D

R

R

K

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Sites not aligning to the query:

222:324 mutation Missing: In cc561; low frequency of embryonic lethality, with embryos arresting paralyzed at the two-fold stage (Pat); increases in frequency significantly on an hnd-1 or unc-120 mutant background. Many embryos that survive to hatch become uncoordinated, dumpy larvae.

P10627 Protein twist from Drosophila melanogaster (Fruit fly) (see paper)
35% identity, 92% coverage: 4:60/62 of query aligns to 361:417/490 of P10627

query
sites
P10627

A

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Sites not aligning to the query:

325 modified: Phosphoserine
328 modified: Phosphoserine

8osbB Twist1-tcf4-alx4 complex on specific DNA (see paper)
38% identity, 90% coverage: 6:61/62 of query aligns to 8:63/66 of 8osbB

query
sites
8osbB

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binding : R9 (= R7), N13 (≠ T11), R15 (= R13), E16 (= E14), E16 (= E14), R17 (= R15), R19 (= R17), T20 (≠ L18), N24 (= N22), K44 (= K42)

P26687 Twist-related protein 1; M-twist from Mus musculus (Mouse) (see paper)
38% identity, 90% coverage: 6:61/62 of query aligns to 113:168/206 of P26687

query
sites
P26687

R

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Sites not aligning to the query:

179 C→G: High transactivation activity.
185 mutation E->D,K,R: High transactivation activity.
186 mutation R->A,K: High transactivation activity.
187 L→G: Low transactivation activity.
188 S→A: High transactivation activity.
191 mutation F->A,G,P: Low transactivation activity.
192 S→A: High transactivation activity.; S→P: Intermediate transactivation activity.
195 R→E: Intermediate transactivation activity.; R→G: Low transactivation activity.

Q9D030 Twist-related protein 2; Dermis-expressed protein 1; Dermo-1 from Mus musculus (Mouse) (see paper)
38% identity, 84% coverage: 10:61/62 of query aligns to 71:122/160 of Q9D030

query
sites
Q9D030

A

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F86 (= F25) mutation to P: Abrogates transcriptional repression of MYOD1.

Query Sequence

>62 a.a. (TTNADRRKAA...)
TTNADRRKAATMRERRRLSKVNEAFETLKRSTSSNPNQRLPKVEILRNAIRYIEGLQALL
RD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory