SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 642684212 IMG__ChlphaBS1_FD:642684212 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

C1DFH7 Ketol-acid reductoisomerase (NADP(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; Ketol-acid reductoisomerase type 1; Ketol-acid reductoisomerase type I; EC 1.1.1.86 from Azotobacter vinelandii (strain DJ / ATCC BAA-1303) (see paper)
60% identity, 99% coverage: 1:327/330 of query aligns to 1:327/338 of C1DFH7

query
sites
C1DFH7

M

M

N

K

A

V

Y

Y

E

D

Q

K

D

D

A

C

D

D

L

L

K

S

Y

I

L

I

Q

Q

G

S

K

K

N

K

I

V

A

A

V

I

L

I

G

G

F

Y

G

G

S

S

Q

Q

G

G

H

H

A

A

H

H

A

A

L

C

N

N

L

L

K

K

E

D

S

S

G

G

L

V

N

D

V

V

C

Y

V

V

G

G

L

L

R

R

P

A

D

G

S

S

S

A

S

S

C

V

E

A

K

K

A

A

R

E

E

A

N

H

G

G

L

L

E

T

V

V

N

K

T

S

I

V

A

K

D

D

A

A

V

V

A

A

W

A

A

A

D

D

I

V

V

V

M

M

V

I

L

L

L

T

P

P

D

D

Q

E

Y

F

Q

Q

K

G

S

R

I

L

Y

Y

E

K

S

D

E

E

I

I

K

E

P

P

N

N

L

L

K

K

E

K

G

G

N

A

T

T

L

L

A

A

F

F

A

A

H

H

G

G

F

F

N

S

I

I

H

H

Y

Y

N

N

Q

Q

I

V

V

V

P

P

P

R

G

A

T

D

V

L

N

D

V

V

I

I

M

M

I

I

A

A

P

P

K

K

S

A

P

P

G

G

H

H

L

T

V

V

R

R

R

S

T

E

Y

F

T

V

Q

R

G

G

N

G

G

G

V

I

P

P

C

D

L

L

I

I

A

A

V

V

H

Y

Q

Q

D

D

Y

A

T

S

G

G

E

N

A

A

R

K

Q

N

Q

L

A

A

L

L

A

S

W

Y

A

A

K

C

G

G

L

V

G

G

T

G

K

R

A

T

G

G

V

I

I

I

E

E

T

T

F

F

K

K

N

D

E

E

T

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E

E

Q

Q

A

A

V

V

L

L

C

C

G

G

S

C

A

V

E

E

L

L

I

V

K

K

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

P

A

E

P

E

E

L

M

A

A

Y

Y

F

F

E
|
E

C

C

M

L

H

H

E
|
E

L

L

K

K

L

L

I

I

V

V

D

D

L

L

Y

M

Y

F

E

E

G

G

L

I

S

A

R

N

M

M

N

N

Y

Y

S

S

V

I

S

S

D

N

T

N

A

A

E

E

Y

Y

G

G

G

E

M

Y

T

V

R

T

G

G

P

P

R

E

V

V

I

I

T

N

S

E

D

Q

V

S

K

R

A

Q

E

A

M

M

K

R

K

N

I

A

L

L

E

K

E

R

V

I

Q

Q

D

D

G

G

R

E

F

Y

A

A

K

K

E

M

F

F

I

I

D

T

E

E

C

G

N

A

A

A

G

N

Y

Y

P

P

N

S

M

M

K

T

K

A

L

Y

R

R

E

R

S

N

N

N

A

A

D

A

H

H

P

Q

I

I

E

E

K

V

V

V

G

G

A

E

K

K

L

L

R

R

D

T

M

M

S

P

W

W

L

I

D190 (= D190) binding Mg(2+)
E226 (= E226) binding Mg(2+)
E230 (= E230) binding Mg(2+)

4xiyA Crystal structure of ketol-acid reductoisomerase from azotobacter (see paper)
60% identity, 99% coverage: 1:327/330 of query aligns to 1:327/328 of 4xiyA

query
sites
4xiyA

M

M

N

K

A

V

Y

Y

E

D

Q

K

D

D

A

C

D

D

L

L

K

S

Y

I

L

I

Q

Q

G

S

K

K

N

K

I

V

A

A

V

I

L

I

G

G

F

Y

G

G

S

S

Q

Q

G

G

H

H

A

A

H

H

A

A

L

C

N

N

L

L

K

K

E

D

S

S

G

G

L

V

N

D

V

V

C

Y

V

V

G

G

L

L

R

R

P

A

D

G

S

S

S

A

S

S

C

V

E

A

K

K

A

A

R

E

E

A

N

H

G

G

L

L

E

T

V

V

N

K

T

S

I

V

A

K

D

D

A

A

V

V

A

A

W

A

A

A

D

D

I

V

V

V

M

M

V

I

L

L

L

T

P

P

D

D

Q

E

Y

F

Q

Q

K

G

S

R

I

L

Y

Y

E

K

S

D

E

E

I

I

K

E

P

P

N

N

L

L

K

K

E

K

G

G

N

A

T

T

L

L

A

A

F

F

A

A

H

H

G

G

F

F

N

S

I

I

H

H

Y

Y

N

N

Q

Q

I

V

V

V

P

P

P

R

G

A

T

D

V

L

N

D

V

V

I

I

M

M

I

I

A

A

P

P

K
|
K

S

A

P

P

G

G

H

H

L

T

V

V

R

R

R

S

T

E

Y

F

T

V

Q

R

G

G

N

G

G

G

V

I

P

P

C

D

L

L

I

I

A

A

V

V

H

Y

Q

Q

D

D

Y

A

T

S

G

G

E

N

A

A

R

K

Q

N

Q

L

A

A

L

L

A

S

W

Y

A

A

K

C

G

G

L

V

G

G

T

G

K

R

A

T

G

G

V

I

I

I

E

E

T

T

F

F

K

K

N

D

E

E

T

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

S

C

A

V

E

E

L

L

I

V

K

K

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

P

A

E

P

E

E

L

M

A

A

Y

Y

F

F

E
|
E

C

C

M

L

H

H

E
|
E

L

L

K

K

L

L

I

I

V

V

D

D

L

L

Y

M

Y

F

E

E

G

G

L

I

S

A

R

N

M

M

N

N

Y

Y

S

S

V

I

S

S

D

N

T

N

A

A

E

E

Y

Y

G

G

G

E

M

Y

T

V

R

T

G

G

P

P

R

E

V

V

I

I

T

N

S

E

D

Q

V

S

K

R

A

Q

E

A

M

M

K

R

K

N

I

A

L

L

E

K

E

R

V

I

Q

Q

D

D

G

G

R

E

F

Y

A

A

K

K

E

M

F

F

I

I

D

T

E

E

C

G

N

A

A

A

G

N

Y

Y

P

P

N

S

M

M

K

T

K

A

L

Y

R

R

E

R

S

N

N

N

A

A

D

A

H

H

P

Q

I

I

E

E

K

V

V

V

G

G

A

E

K

K

L

L

R

R

D

T

M

M

S

P

W

W

L

I

active site: K130 (= K130), D190 (= D190), E194 (= E194)
binding fe (iii) ion: D190 (= D190), E194 (= E194)
binding magnesium ion: E226 (= E226), E230 (= E230)

P9WKJ7 Ketol-acid reductoisomerase (NADP(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; Ketol-acid reductoisomerase type 1; Ketol-acid reductoisomerase type I; EC 1.1.1.86 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
58% identity, 99% coverage: 1:327/330 of query aligns to 3:329/337 of P9WKJ7

query
sites
P9WKJ7

M

L

N

E

A

M

Y

F

Y

Y

E

D

Q

D

D

D

A

A

D

D

L

L

K

S

Y

I

L

I

Q

Q

G

G

K

R

N

K

I

V

A

G

V

V

L

I

G

G

F

Y

G

G

S

S

Q

Q

G

G

H

H

A

A

H

H

A

S

L

L

N

S

L

L

K

R

E

D

S

S

G

G

L

V

N

Q

V

V

C

R

V

V

G

G

L

L

R

K

P

Q

D

G

S

S

S

R

S

S

C

R

E

P

K

K

A

V

R

E

E

E

N

Q

G

G

L

L

E

D

V

V

N

D

T

T

I

P

A

A

D

E

A

V

V

A

A

K

W

W

A

A

D

D

I

V

V

V

M

M

V

V

L

L

L

A

P

P

D

D

Q

T

Y

A

Q

Q

K

A

S

E

I

I

Y

F

E

A

S

G

E

D

I

I

K

E

P

P

N

N

L

L

K

K

E

P

G

G

N

D

T

A

L

L

A

F

F

F

A

G

H

H

G

G

F

L

N

N

I

V

H

H

Y

F

N

G

Q

L

I

I

V

K

P

P

G

A

T

D

V

V

N

A

V

V

I

A

M

M

I

V

A

A

P

P

K

K

S

G

P

P

G

G

H

H

L

L

V

V

R

R

T

Q

Y

F

T

V

Q

D

G

G

N

K

G

G

V

V

P

P

C

C

L

L

I

V

A

A

V

V

H

E

Q

Q

D

D

Y

P

T

R

G

G

E

D

A

G

R

L

Q

A

Q

L

A

A

L

L

A

S

W

Y

A

A

K

K

G

A

L

I

G

G

T

T

K

R

A

A

G

G

V

V

I

I

E

K

T

T

F

F

K

K

N

D

E

E

T

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

T

V

V

L

L

C

C

G

G

S

T

A

E

E

E

L

L

I

V

K

K

A

A

G

G

F

F

E

E

T

V

L

M

V

V

E

E

A

A

G

G

Y

Y

P

P

E

A

E

E

L

L

A

A

Y

Y

F

F

E
|
E

C

V

M

L

H

H

E
|
E

L

L

K

K

L

L

I

I

V

V

D

D

L

L

Y

M

Y

Y

E

E

G

G

L

L

S

A

R

R

M

M

N

Y

Y

Y

S

S

V

V

S

S

D

D

T

T

A

A

E

E

Y

F

G

G

M

Y

T

L

R

S

G

G

P

P

R

R

V

V

I

I

T

D

S

A

D

G

V

T

K

K

A

E

E

R

M

M

K

R

K

D

I

I

L

L

E

R

E

E

V

I

Q

Q

D

D

G

G

R

S

F

F

A

V

K

H

E

K

F

L

I

V

D

A

E

D

C

V

N

E

A

G

G

G

Y

N

P

K

N

Q

M

L

K

E

L

L

R

R

E

R

S

Q

N

N

A

A

D

E

H

H

P

P

I

I

E

E

K

V

V

V

G

G

A

K

K

K

L

L

R

R

D

D

M

L

M

M

S

S

W

W

L

V

D192 (= D190) binding Mg(2+); binding Mg(2+)
E196 (= E194) binding Mg(2+)
E228 (= E226) binding Mg(2+)
E232 (= E230) binding Mg(2+)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4ypoA Crystal structure of mycobacterium tuberculosis ketol-acid reductoisomerase in complex with mg2+ (see paper)
59% identity, 98% coverage: 4:327/330 of query aligns to 2:325/325 of 4ypoA

query
sites
4ypoA

Y

F

Y

Y

E

D

Q

D

D

D

A

A

D

D

L

L

K

S

Y

I

L

I

Q

Q

G

G

K

R

N

K

I

V

A

G

V

V

L

I

G

G

F

Y

G

G

S

S

Q

Q

G

G

H

H

A

A

H

H

A

S

L

L

N

S

L

L

K

R

E

D

S

S

G

G

L

V

N

Q

V

V

C

R

V

V

G

G

L

L

R

K

P

Q

D

G

S

S

S

R

S

S

C

R

E

P

K

K

A

V

R

E

E

E

N

Q

G

G

L

L

E

D

V

V

N

D

T

T

I

P

A

A

D

E

A

V

V

A

A

K

W

W

A

A

D

D

I

V

V

V

M

M

V

V

L

L

L

A

P

P

D

D

Q

T

Y

A

Q

Q

K

A

S

E

I

I

Y

F

E

A

S

G

E

D

I

I

K

E

P

P

N

N

L

L

K

K

E

P

G

G

N

D

T

A

L

L

A

F

F

F

A

G

H

H

G

G

F

L

N

N

I

V

H

H

Y

F

N

G

Q

L

I

I

V

K

P

P

G

A

T

D

V

V

N

A

V

V

I

A

M

M

I

V

A

A

P

P

K
|
K

S

G

P

P

G

G

H

H

L

L

V

V

R

R

T

Q

Y

F

T

V

Q

D

G

G

N

K

G

G

V

V

P

P

C

C

L

L

I

V

A

A

V

V

H

E

Q

Q

D

D

Y

P

T

R

G

G

E

D

A

G

R

L

Q

A

Q

L

A

A

L

L

A

S

W

Y

A

A

K

K

G

A

L

I

G

G

T

T

K

R

A

A

G

G

V

V

I

I

E

K

T

T

F

F

K

K

N

D

E

E

T

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

T

V

V

L

L

C

C

G

G

S

T

A

E

E

E

L

L

I

V

K

K

A

A

G

G

F

F

E

E

T

V

L

M

V

V

E

E

A

A

G

G

Y

Y

P

P

E

A

E

E

L

L

A

A

Y

Y

F

F

E
|
E

C

V

M

L

H

H

E
|
E

L

L

K

K

L

L

I

I

V

V

D

D

L

L

Y

M

Y

Y

E

E

G

G

L

L

S

A

R

R

M

M

N

Y

Y

Y

S

S

V

V

S

S

D

D

T

T

A

A

E

E

Y

F

G

G

M

Y

T

L

R

S

G

G

P

P

R

R

V

V

I

I

T

D

S

A

D

G

V

T

K

K

A

E

E

R

M

M

K

R

K

D

I

I

L

L

E

R

E

E

V

I

Q

Q

D

D

G

G

R

S

F

F

A

V

K

H

E

K

F

L

I

V

D

A

E

D

C

V

N

E

A

G

G

G

Y

N

P

K

N

Q

M

L

K

E

L

L

R

R

E

R

S

Q

N

N

A

A

D

E

H

H

P

P

I

I

E

E

K

V

V

V

G

G

A

K

K

K

L

L

R

R

D

D

M

L

M

M

S

S

W

W

L

V

active site: K128 (= K130), D188 (= D190), E192 (= E194)
binding magnesium ion: D188 (= D190), E192 (= E194), E224 (= E226), E228 (= E230)

8upqB Campylobacter jejuni ketol-acid reductoisomerase in complex with 2,3- dihydroxy-3-isovalerate.
56% identity, 100% coverage: 1:330/330 of query aligns to 2:331/342 of 8upqB

query
sites
8upqB

M

I

N

T

A

V

Y

Y

E

D

Q

K

D

D

A

C

D

D

L

L

K

N

Y

L

L

I

Q

K

G

S

K

K

N

K

I

V

A

A

V

I

L

I

G

G

F

F

G

G

S

S

Q

Q

G

G

H

H

A

A

H

H

A

A

L

M

N

N

L

L

K

R

E

D

S

N

G

G

L

V

N

N

V

V

C

T

V

I

G

G

L

L

R

R

P

E

D

G

S

S

S

V

S

S

C

A

E

V

K

K

A

A

R

K

E

N

N

A

G

G

L

F

E

E

V

V

N

M

T

S

I

V

A

S

D

E

A

A

V

S

A

K

W

I

A

A

D

D

I

V

V

I

M

M

V

I

L

L

L

A

P

P

D

D

Q

E

Y

I

Q

Q

K

A

S

D

I

I

Y

F

E

N

S

V

E

E

I

I

K

K

P

P

N

N

L

L

K

S

E

E

G

G

N

K

T

A

L

I

A

A

F

F

A

A

H

H

G

G

F

F

N

N

I

I

H

H

Y

Y

N

G

Q

Q

I

I

V

V

P

V

P

P

G

K

T

G

V

V

N

D

V

V

I

I

M

M

I

I

A

A

P

P

K

K

S
x
A

P
|
P

G

G

H

H

L

T

V

V

R

R

R

N

T

E

Y

F

T

T

Q

L

G

G

N

G

G

G

V

T

P

P

C

C

L

L

I

I

A

A

V

I

H

H

Q

Q

D

D

Y

E

T

S

G

K

E

N

A

A

R

K

Q

N

Q

L

A

A

L

L

A

S

W

Y

A

A

K

S

G

A

L

I

G

G

T

G

K

R

A

T

G

G

V

I

I

I

E

E

T

T

F

F

K

K

N

A

E

E

T

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

S

L

A

S

E

A

L

L

I

I

K

Q

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

P

E

E

P

E

E

L

M

A

A

Y

Y

F

F

E

E

C

C

M

L

H

H

E
|
E

L

M

K

K

L

L

I

I

V

V

D

D

L

L

Y

I

Y

Y

E

Q

G

G

L

I

S

A

R

D

M

M

N

R

Y

Y

S

S

V

I

S
|
S

D

N

T

T

A
|
A

E

E

Y

Y

G

G

G

D

M

Y

T

I

R

T

G

G

P

P

R

K

V

I

I

I

T

T

S

E

D

E

V

T

K

K

A

K

E

A

M

M

K

K

K

G

I

V

L

L

E

K

E

D

V

I

Q

Q

D

N

G

G

R

V

F

F

A

A

K

K

E

D

F

F

I

I

D

L

E

E

C

R

N

R

A

A

G

G

Y

F

P

A

N

R

M

M

K

H

K

A

L

E

R

R

E

K

S

N

N

M

A

N

D

D

H

S

P

L

I

I

E

E

K

K

V

T

G

G

A

R

K

N

L

L

R

R

D

A

M

M

S

P

W

W

L

I

L

S

K

A

K

K

binding magnesium ion: D191 (= D190), E195 (= E194)
binding (2R)-2,3-dihydroxy-3-methylbutanoic acid: A132 (≠ S131), P133 (= P132), D191 (= D190), E195 (= E194), E231 (= E230), S252 (= S251), A255 (= A254)

7rduA Crystal structure of campylobacter jejuni keto said reductoisomerase in complex with magnesium and oxidixized and reduced NADPH
57% identity, 99% coverage: 1:327/330 of query aligns to 2:328/329 of 7rduA

query
sites
7rduA

M

I

N

T

A

V

Y

Y

E

D

Q

K

D

D

A

C

D

D

L

L

K

N

Y

L

L

I

Q

K

G

S

K

K

N

K

I

V

A

A

V

I

L

I

G
|
G

F
|
F

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

L

M

N

N

L

L

K

R

E

D

S

N

G

G

L

V

N

N

V

V

C

T

V

I

G

G

L

L

R
|
R

P

E

D

G

S
|
S

S

V

S
|
S

C

A

E

V

K

K

A

A

R

K

E

N

N

A

G

G

L

F

E

E

V

V

N

M

T

S

I

V

A

S

D

E

A

A

V

S

A

K

W

I

A

A

D

D

I

V

V

I

M

M

V

I

L
|
L

L
x
A

P
|
P

D
|
D

Q

E

Y

I

Q

Q

K

A

S

D

I
|
I

Y

F

E

N

S

V

E

E

I

I

K

K

P

P

N

N

L

L

K

S

E

E

G

G

N

K

T

A

L

I

A

A

F

F

A
|
A

H
|
H

G

G

F

F

N

N

I

I

H

H

Y

Y

N

G

Q

Q

I

I

V

V

P

V

P

P

G

K

T

G

V

V

N

D

V

V

I

I

M

M

I

I

A

A

P
|
P

K
|
K

S
x
A

P

P

G

G

H

H

L

T

V

V

R

R

R

N

T

E

Y

F

T

T

Q

L

G

G

N

G

G

G

V

T

P

P

C

C

L

L

I

I

A

A

V

I

H

H

Q

Q

D

D

Y

E

T

S

G

K

E

N

A

A

R

K

Q

N

Q

L

A

A

L

L

A

S

W

Y

A

A

K

S

G

A

L

I

G

G

T

G

K

R

A

T

G

G

V

I

I

I

E

E

T

T

F

F

K

K

N

A

E

E

T

T

E

E

T

T

D

D

L

L

F

F

G

G

E

E

Q

Q

A

A

V

V

L

L

C

C

G

G

S

L

A

S

E

A

L

L

I

I

K

Q

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

P

E

E

P

E

E

L

M

A

A

Y

Y

F

F

E

E

C

C

M

L

H

H

E

E

L

M

K

K

L

L

I

I

V

V

D

D

L

L

Y

I

Y

Y

E

Q

G

G

L

I

S

A

R

D

M

M

N

R

Y

Y

S

S

V

I

S

S

D

N

T

T

A

A

E

E

Y

Y

G

G

G

D

M

Y

T

I

R

T

G

G

P

P

R

K

V

I

I

I

T

T

S

E

D

E

V

T

K

K

A

K

E

A

M

M

K

K

K

G

I

V

L

L

E

K

E

D

V

I

Q

Q

D

N

G

G

R

V

F

F

A

A

K

K

E

D

F

F

I

I

D

L

E

E

C

R

N

R

A

A

G

G

Y

F

P

A

N

R

M

M

K

H

K

A

L

E

R

R

E

K

S

N

N

M

A

N

D

D

H

S

P

L

I

I

E

E

K

K

V

T

G

G

A

R

K

N

L

L

R

R

D

A

M

M

S

P

W

W

L

I

binding nadp nicotinamide-adenine-dinucleotide phosphate: G24 (= G23), G26 (= G25), S27 (= S26), Q28 (= Q27), R48 (= R47), S51 (= S50), S53 (= S52), A81 (≠ L80), P82 (= P81), D83 (= D82), I89 (= I88), A107 (= A106), H108 (= H107), P130 (= P129), K131 (= K130), A132 (≠ S131)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G24 (= G23), F25 (= F24), G26 (= G25), S27 (= S26), Q28 (= Q27), S51 (= S50), S53 (= S52), L80 (= L79), P82 (= P81), D83 (= D82), I89 (= I88), A107 (= A106), H108 (= H107)

8uppA Campylobacter jejuni ketol-acid reductoisomerase in complex with NADPH and hoe704
57% identity, 99% coverage: 1:327/330 of query aligns to 1:327/327 of 8uppA

query
sites
8uppA

M

I

N

T

A

V

Y

Y

E

D

Q

K

D

D

A

C

D

D

L

L

K

N

Y

L

L

I

Q

K

G

S

K

K

N

K

I

V

A

A

V

I

L

I

G

G

F
|
F

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

L

M

N

N

L

L

K

R

E

D

S

N

G

G

L

V

N

N

V

V

C

T

V

I

G

G

L

L

R
|
R

P

E

D

G

S
|
S

S

V

S

S

C

A

E

V

K

K

A

A

R

K

E

N

N

A

G

G

L

F

E

E

V

V

N

M

T

S

I

V

A

S

D

E

A

A

V

S

A

K

W

I

A

A

D

D

I

V

V

I

M

M

V

I

L
|
L

L

A

P
|
P

D
|
D

Q

E

Y
x
I

Q
|
Q

K

A

S

D

I
|
I

Y

F

E

N

S

V

E

E

I

I

K

K

P

P

N

N

L

L

K

S

E

E

G

G

N

K

T

A

L

I

A

A

F

F

A

A

H
|
H

G

G

F

F

N

N

I

I

H

H

Y

Y

N

G

Q

Q

I

I

V

V

P

V

P

P

G

K

T

G

V

V

N

D

V

V

I

I

M

M

I

I

A

A

P

P

K

K

S
x
A

P
|
P

G

G

H

H

L

T

V

V

R

R

R

N

T

E

Y

F

T

T

Q

L

G

G

N

G

G

G

V

T

P

P

C

C

L

L

I

I

A

A

V

I

H

H

Q

Q

D

D

Y

E

T

S

G

K

E

N

A

A

R

K

Q

N

Q

L

A

A

L

L

A

S

W

Y

A

A

K

S

G

A

L

I

G

G

T

G

K

R

A

T

G

G

V

I

I

I

E

E

T

T

F

F

K

K

N

A

E

E

T

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

S

L

A

S

E

A

L

L

I

I

K

Q

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

P

E

E

P

E

E

L

M

A

A

Y

Y

F

F

E

E

C

C

M

L

H

H

E
|
E

L

M

K

K

L

L

I

I

V

V

D

D

L

L

Y

I

Y

Y

E

Q

G

G

L

I

S

A

R

D

M

M

N

R

Y

Y

S

S

V
x
I

S
|
S

D

N

T

T

A
|
A

E

E

Y

Y

G

G

G

D

M

Y

T

I

R

T

G

G

P

P

R

K

V

I

I

I

T

T

S

E

D

E

V

T

K

K

A

K

E

A

M

M

K

K

K

G

I

V

L

L

E

K

E

D

V

I

Q

Q

D

N

G

G

R

V

F

F

A

A

K

K

E

D

F

F

I

I

D

L

E

E

C

R

N

R

A

A

G

G

Y

F

P

A

N

R

M

M

K

H

K

A

L

E

R

R

E

K

S

N

N

M

A

N

D

D

H

S

P

L

I

I

E

E

K

K

V

T

G

G

A

R

K

N

L

L

R

R

D

A

M

M

S

P

W

W

L

I

binding magnesium ion: D190 (= D190), E194 (= E194)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F24 (= F24), G25 (= G25), S26 (= S26), Q27 (= Q27), R47 (= R47), S50 (= S50), L79 (= L79), P81 (= P81), D82 (= D82), I84 (≠ Y84), Q85 (= Q85), I88 (= I88), H107 (= H107), P132 (= P132), I250 (≠ V250), S251 (= S251)
binding (2R)-(dimethylphosphoryl)(hydroxy)acetic acid: A131 (≠ S131), P132 (= P132), D190 (= D190), E194 (= E194), E230 (= E230), I250 (≠ V250), S251 (= S251), A254 (= A254)

8sxdA Campylobacter jejuni keto-acid reductoisomerase in complex with intermediate and NADP+
57% identity, 99% coverage: 1:327/330 of query aligns to 1:327/327 of 8sxdA

query
sites
8sxdA

M

I

N

T

A

V

Y

Y

E

D

Q

K

D

D

A

C

D

D

L

L

K

N

Y

L

L

I

Q

K

G

S

K

K

N

K

I

V

A

A

V

I

L

I

G
|
G

F
|
F

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

L

M

N

N

L

L

K

R

E

D

S

N

G

G

L

V

N

N

V

V

C

T

V

I

G

G

L

L

R

R

P

E

D

G

S
|
S

S

V

S
|
S

C

A

E

V

K

K

A

A

R

K

E

N

N

A

G

G

L

F

E

E

V

V

N

M

T

S

I

V

A

S

D

E

A

A

V

S

A

K

W

I

A

A

D

D

I

V

V

I

M

M

V

I

L
|
L

L
x
A

P
|
P

D
|
D

Q

E

Y

I

Q

Q

K

A

S

D

I

I

Y

F

E

N

S

V

E

E

I

I

K

K

P

P

N

N

L

L

K

S

E

E

G

G

N

K

T

A

L

I

A

A

F

F

A

A

H

H

G

G

F

F

N

N

I

I

H

H

Y

Y

N

G

Q

Q

I

I

V

V

P

V

P

P

G

K

T

G

V

V

N

D

V

V

I

I

M

M

I

I

A

A

P
|
P

K

K

S

A

P

P

G

G

H

H

L

T

V

V

R

R

R

N

T

E

Y

F

T

T

Q

L

G

G

N

G

G

G

V

T

P

P

C

C

L

L

I

I

A

A

V

I

H

H

Q

Q

D

D

Y

E

T

S

G

K

E

N

A

A

R

K

Q

N

Q

L

A

A

L

L

A

S

W

Y

A

A

K

S

G

A

L

I

G

G

T

G

K

R

A

T

G

G

V

I

I

I

E

E

T

T

F

F

K

K

N

A

E

E

T

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

S

L

A

S

E

A

L

L

I

I

K

Q

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

P

E

E

P

E

E

L

M

A

A

Y

Y

F

F

E

E

C

C

M

L

H

H

E

E

L

M

K

K

L

L

I

I

V

V

D

D

L

L

Y

I

Y

Y

E

Q

G

G

L

I

S

A

R

D

M

M

N

R

Y

Y

S

S

V

I

S

S

D

N

T

T

A

A

E

E

Y

Y

G

G

G

D

M

Y

T

I

R

T

G

G

P

P

R

K

V

I

I

I

T

T

S

E

D

E

V

T

K

K

A

K

E

A

M

M

K

K

K

G

I

V

L

L

E

K

E

D

V

I

Q

Q

D

N

G

G

R

V

F

F

A

A

K

K

E

D

F

F

I

I

D

L

E

E

C

R

N

R

A

A

G

G

Y

F

P

A

N

R

M

M

K

H

K

A

L

E

R

R

E

K

S

N

N

M

A

N

D

D

H

S

P

L

I

I

E

E

K

K

V

T

G

G

A

R

K

N

L

L

R

R

D

A

M

M

S

P

W

W

L

I

binding magnesium ion: D190 (= D190), E194 (= E194)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G23 (= G23), F24 (= F24), G25 (= G25), S26 (= S26), Q27 (= Q27), S50 (= S50), S52 (= S52), L79 (= L79), A80 (≠ L80), P81 (= P81), D82 (= D82), P129 (= P129)
binding 3-hydroxy-3-methyl-2-oxobutanoic acid: D190 (= D190), E194 (= E194)

8swmA Crystal structure of campylobacter jejuni ketol-acid reductoisomerase in complex with 2-acetolactate
57% identity, 98% coverage: 4:326/330 of query aligns to 1:323/323 of 8swmA

query
sites
8swmA

Y

Y

E

D

Q

K

D

D

A

C

D

D

L

L

K

N

Y

L

L

I

Q

K

G

S

K

K

N

K

I

V

A

A

V

I

L

I

G

G

F

F

G

G

S

S

Q

Q

G

G

H

H

A

A

H

H

A

A

L

M

N

N

L

L

K

R

E

D

S

N

G

G

L

V

N

N

V

V

C

T

V

I

G

G

L

L

R

R

P

E

D

G

S

S

S

V

S

S

C

A

E

V

K

K

A

A

R

K

E

N

N

A

G

G

L

F

E

E

V

V

N

M

T

S

I

V

A

S

D

E

A

A

V

S

A

K

W

I

A

A

D

D

I

V

V

I

M

M

V

I

L

L

L

A

P

P

D

D

Q

E

Y

I

Q

Q

K

A

S

D

I

I

Y

F

E

N

S

V

E

E

I

I

K

K

P

P

N

N

L

L

K

S

E

E

G

G

N

K

T

A

L

I

A

A

F

F

A

A

H

H

G

G

F

F

N

N

I

I

H

H

Y

Y

N

G

Q

Q

I

I

V

V

P

V

P

P

G

K

T

G

V

V

N

D

V

V

I

I

M

M

I

I

A

A

P

P

K

K

S

A

P

P

G

G

H

H

L

T

V

V

R

R

R

N

T

E

Y

F

T

T

Q

L

G

G

N

G

G

G

V

T

P

P

C

C

L

L

I

I

A

A

V

I

H

H

Q

Q

D

D

Y

E

T

S

G

K

E

N

A

A

R

K

Q

N

Q

L

A

A

L

L

A

S

W

Y

A

A

K

S

G

A

L

I

G

G

T

G

K

R

A

T

G

G

V

I

I

I

E

E

T

T

F

F

K

K

N

A

E

E

T

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

S

L

A

S

E

A

L

L

I

I

K

Q

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

P

E

E

P

E

E

L

M

A

A

Y

Y

F

F

E

E

C

C

M

L

H

H

E

E

L

M

K

K

L

L

I

I

V

V

D

D

L

L

Y

I

Y

Y

E

Q

G

G

L

I

S

A

R

D

M

M

N

R

Y

Y

S

S

V

I

S

S

D

N

T

T

A

A

E

E

Y

Y

G

G

G

D

M

Y

T

I

R

T

G

G

P

P

R

K

V

I

I

I

T

T

S

E

D

E

V

T

K

K

A

K

E

A

M

M

K

K

K

G

I

V

L

L

E

K

E

D

V

I

Q

Q

D

N

G

G

R

V

F

F

A

A

K

K

E

D

F

F

I

I

D

L

E

E

C

R

N

R

A

A

G

G

Y

F

P

A

N

R

M

M

K

H

K

A

L

E

R

R

E

K

S

N

N

M

A

N

D

D

H

S

P

L

I

I

E

E

K

K

V

T

G

G

A

R

K

N

L

L

R

R

D

A

M

M

S

P

W

W

binding magnesium ion: D187 (= D190), E191 (= E194)
binding (2S)-2-hydroxy-2-methyl-3-oxobutanoic acid: D187 (= D190), E191 (= E194)

D0WGK0 Ketol-acid reductoisomerase (NADP(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; Ketol-acid reductoisomerase type 1; Ketol-acid reductoisomerase type I; EC 1.1.1.86 from Slackia exigua (strain ATCC 700122 / DSM 15923 / CIP 105133 / JCM 11022 / KCTC 5966 / S-7) (see paper)
58% identity, 99% coverage: 5:330/330 of query aligns to 16:341/342 of D0WGK0

query
sites
D0WGK0

Y

Y

E

E

Q

Q

D

D

A

V

D

D

L

P

K

K

Y

V

L

I

Q

Q

G

G

K

L

N

K

I

V

A

G

V

I

L

I

G

G

F
x
Y

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

L

L

N

N

L

L

K

M

E

D

S

S

G

G

L

V

N

D

V

V

C

R

V

V

G

G

L

L

R
|
R

P

E

D

G

S
|
S

S

S

S
|
S

C

W

E

K

K

T

A

A

R

E

E

E

N

A

G

G

L

L

E

K

V

V

N

T

T

D

I

M

A

D

D

T

A

A

V

A

A

E

W

E

A

A

D

D

I

V

V

I

M

M

V

V

L

L

L

V

P

P

D
|
D

Q
x
E

Y
x
I

Q
|
Q

K

P

S

K

I

V

Y

Y

E

Q

S

E

E

H

I

I

K

A

P

A

N

H

L

L

K

K

E

A

G

G

N

N

T

T

L

L

A

A

F

F

A

A

H

H

G

G

F

F

N

N

I

I

H

H

Y

Y

N

G

Q

Y

I

I

V

V

P

P

G

E

T

D

V

V

N

N

V

V

I

I

M

M

I

C

A

A

P

P

K

K

S

G

P

P

G
|
G

H

H

L

I

V

V

R

R

T

Q

Y

F

T

T

Q

E

G

G

N

S

G

G

V

V

P

P

C

D

L

L

I

A

A

C

V

V

H

Q

Q

Q

D

D

Y

A

T

T

G

G

E

N

A

A

R

W

Q

D

Q

I

A

V

L

L

A

S

W

Y

A

C

K

W

G

G

L

V

G

G

T

A

K

R

A

S

G

G

V

I

I

I

E

K

T

A

T

T

F

F

K

A

N

E

E

E

T

T

E

E

T

E

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

S

L

A

V

E

E

L

L

I

V

K

K

A

A

G

G

F

F

E

E

T

T

L

L

V

T

E

E

A

A

G

G

Y

Y

P

P

E

P

E

E

L

L

A

A

Y

Y

F

F

E

E

C

C

M

Y

H

H

E

E

L

M

K

K

L

M

I

I

V

V

D

D

L

L

Y

M

Y

Y

E

E

G

S

G

G

L

I

S

H

R

F

M

M

N

N

Y

Y

S

S

V

I

S
|
S

D

N

T

T

A

A

E

E

Y

Y

G

G

G

E

M

Y

T

Y

R

A

G

G

P

P

R

K

V

V

I

I

T

N

S

E

D

Q

V

S

K

R

A

E

E

A

M

M

K

K

K

E

I

I

L

L

E

K

E

R

V

I

Q

Q

D

D

G

G

R

S

F

F

A

A

K

Q

E

E

F

F

I

V

D

D

E

D

C

C

N

N

A

N

G

G

Y

H

P

K

N

R

M

L

K

L

K

E

L

Q

R

R

E

E

S

A

N

I

A

N

D

T

H

H

P

P

I

I

E

E

K

T

V

T

G

G

A

A

K

Q

L

I

R

R

D

S

M

M

M

F

S

S

W

W

L

I

L

K

K

K

K

E

YGSQ 35:38 (≠ FGSQ 24:27) binding NADP(+)
R58 (= R47) binding NADP(+)
S61 (= S50) binding NADP(+); mutation to D: Inversion of cofactor specificity from NADPH to NADH. Strong decrease of the affinity for NADPH and 2.5-fold decrease of the affinity for NADH. 8-fold decrease of the catalytic efficiency for NADPH and 2-fold increase of the catalytic efficiency for NADH; when associated with D-63.
S63 (= S52) binding NADP(+); mutation to D: Inversion of cofactor specificity from NADPH to NADH. Strong decrease of the affinity for NADPH and 2.5-fold decrease of the affinity for NADH. 8-fold decrease of the catalytic efficiency for NADPH and 2-fold increase of the catalytic efficiency for NADH; when associated with D-61.
DEIQ 93:96 (≠ DQYQ 82:85) binding NADP(+)
G144 (= G133) binding NADP(+)
D201 (= D190) binding Mg(2+)
E205 (= E194) binding Mg(2+)
S262 (= S251) binding substrate

4kqwA The structure of the slackia exigua kari in complex with NADP (see paper)
58% identity, 98% coverage: 5:327/330 of query aligns to 4:326/326 of 4kqwA

query
sites
4kqwA

Y

Y

E

E

Q

Q

D

D

A

V

D

D

L

P

K

K

Y

V

L

I

Q

Q

G

G

K

L

N

K

I

V

A

G

V

I

L

I

G

G

F
x
Y

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

L

L

N

N

L

L

K

M

E

D

S

S

G

G

L

V

N

D

V

V

C

R

V

V

G

G

L

L

R
|
R

P

E

D

G

S
|
S

S

S

S
|
S

C

W

E

K

K

T

A

A

R

E

E

E

N

A

G

G

L

L

E

K

V

V

N

T

T

D

I

M

A

D

D

T

A

A

V

A

A

E

W

E

A

A

D

D

I

V

V

I

M

M

V

V

L
|
L

L
x
V

P
|
P

D
|
D

Q

E

Y
x
I

Q
|
Q

K

P

S

K

I

V

Y

Y

E

Q

S

E

E

H

I

I

K

A

P

A

N

H

L

L

K

K

E

A

G

G

N

N

T

T

L

L

A

A

F

F

A

A

H
|
H

G

G

F

F

N

N

I

I

H

H

Y

Y

N

G

Q

Y

I

I

V

V

P

P

G

E

T

D

V

V

N

N

V

V

I

I

M

M

I

C

A

A

P

P

K
|
K

S

G

P
|
P

G

G

H

H

L

I

V

V

R

R

T

Q

Y

F

T

T

Q

E

G

G

N

S

G

G

V

V

P

P

C

D

L

L

I

A

A

C

V

V

H

Q

Q

Q

D

D

Y

A

T

T

G

G

E

N

A

A

R

W

Q

D

Q

I

A

V

L

L

A

S

W

Y

A

C

K

W

G

G

L

V

G

G

T

A

K

R

A

S

G

G

V

I

I

I

E

K

T

A

T

T

F

F

K

A

N

E

E

E

T

T

E

E

T

E

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

S

L

A

V

E

E

L

L

I

V

K

K

A

A

G

G

F

F

E

E

T

T

L

L

V

T

E

E

A

A

G

G

Y

Y

P

P

E

P

E

E

L

L

A

A

Y

Y

F

F

E

E

C

C

M

Y

H

H

E

E

L

M

K

K

L

M

I

I

V

V

D

D

L

L

Y

M

Y

Y

E

E

G

S

G

G

L

I

S

H

R

F

M

M

N

N

Y

Y

S

S

V
x
I

S
|
S

D

N

T

T

A

A

E

E

Y

Y

G

G

G

E

M

Y

T

Y

R

A

G

G

P

P

R

K

V

V

I

I

T

N

S

E

D

Q

V

S

K

R

A

E

E

A

M

M

K

K

K

E

I

I

L

L

E

K

E

R

V

I

Q

Q

D

D

G

G

R

S

F

F

A

A

K

Q

E

E

F

F

I

V

D

D

E

D

C

C

N

N

A

N

G

G

Y

H

P

K

N

R

M

L

K

L

K

E

L

Q

R

R

E

E

S

A

N

I

A

N

D

T

H

H

P

P

I

I

E

E

K

T

V

T

G

G

A

A

K

Q

L

I

R

R

D

S

M

M

M

F

S

S

W

W

L

I

active site: K129 (= K130), D189 (= D190), E193 (= E194)
binding magnesium ion: D189 (= D190), E193 (= E194)
binding nadp nicotinamide-adenine-dinucleotide phosphate: Y23 (≠ F24), G24 (= G25), S25 (= S26), Q26 (= Q27), R46 (= R47), S49 (= S50), S51 (= S52), L78 (= L79), V79 (≠ L80), P80 (= P81), D81 (= D82), I83 (≠ Y84), Q84 (= Q85), H106 (= H107), P131 (= P132), I249 (≠ V250), S250 (= S251)

8ep7C Crystal structure of the ketol-acid reductoisomerase from bacillus anthracis in complex with NADP
59% identity, 99% coverage: 1:327/330 of query aligns to 1:326/328 of 8ep7C

query
sites
8ep7C

M

M

N

K

A

T

Y

Y

E

E

Q

Q

D

D

A

A

D

N

L

V

K

G

Y

L

L

L

Q

Q

G

G

K

K

N

T

I

V

A

A

V

V

L

I

G

G

F
x
Y

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

Q

A

A

L

Q

N

N

L

L

K

R

E

D

S

S

G

G

L

V

N

E

V

V

C

V

V

V

G

G

L

V

R
|
R

P

P

D

-

S

G

S

K

S
|
S

C

F

E

E

K

V

A

A

R

K

E

A

N

D

G

G

L

F

E

E

V

V

N

M

T

S

I

V

A

S

D

E

A

A

V

V

A

R

W

T

A

A

D

Q

I

V

V

V

M

Q

V

M

L
|
L

P
|
P

D
|
D

Q

E

Y
x
Q

Q

Q

K

A

S

H

I
x
V

Y

Y

E

K

S

A

E

E

I

V

K

E

P

E

N

N

L

L

K

R

E

E

G

G

N

Q

T

M

L

L

A

L

F

F

A

S

H
|
H

G

G

F

F

N

N

I

I

H

H

Y

F

N

G

Q

Q

I

I

V

N

P

P

G

S

T

Y

V

V

N

D

V

V

I

A

M

M

I

V

A

A

P

P

K

K

S

S

P

P

G

G

H

H

L

L

V

V

R

R

T

V

Y

F

T

Q

Q

E

G

G

N

N

G

G

V

V

P

P

C

A

L

L

I

V

A

A

V

V

H

H

Q

Q

D

D

Y

A

T

T

G

G

E

T

A

A

R

L

Q

H

Q

V

A

A

L

L

A

A

W

Y

A

A

K

K

G

G

L

V

G

G

G

C

T

T

K

R

A

A

G

G

V

V

I

I

E

E

T

T

F

F

K

Q

N

E

E

E

T

T

E

E

T

T

D

D

L

L

F

F

G

G

E

E

Q

Q

A

A

V

V

L

L

C

C

G

G

S

V

A

T

E

A

L

L

I

V

K

K

A

A

G

G

F

F

E

E

T

T

L

L

V

T

E

E

A

G

G

G

Y

Y

P

R

E

P

E

E

L

I

A

A

Y

Y

F

F

E

E

C

C

M

L

H

H

E

E

L

L

K

K

L

L

I

I

V

V

D

D

L

L

Y

M

Y

Y

E

E

G

G

L

L

S

T

R

N

M

M

N

R

Y

H

S

S

V

I

S

S

D

D

T

T

A

A

E

E

Y

F

G

G

G

D

M

Y

T

V

R

T

G

G

P

S

R

R

V

I

I

V

T

T

S

D

D

E

V

T

K

K

A

K

E

E

M

M

K

K

K

R

I

V

L

L

E

T

E

E

V

I

Q

Q

D

Q

G

G

R

E

F

F

A

A

K

K

E

K

F

W

I

I

D

L

E

E

C

N

N

Q

A

A

G

G

Y

R

P

P

N

T

M

Y

K

N

K

A

L

M

R

K

E

K

S

A

N

E

A

Q

D

N

H

H

P

Q

I

L

E

E

K

K

V

V

G

G

A

E

K

E

L

L

R

R

D

E

M

M

S

S

W

W

L

I

binding nadp nicotinamide-adenine-dinucleotide phosphate: Y24 (≠ F24), G25 (= G25), S26 (= S26), Q27 (= Q27), R47 (= R47), S51 (= S52), L78 (= L79), L79 (= L80), P80 (= P81), D81 (= D82), Q83 (≠ Y84), V87 (≠ I88), H106 (= H107)

4kqxB Mutant slackia exigua kari ddv in complex with NAD and an inhibitor (see paper)
57% identity, 99% coverage: 5:330/330 of query aligns to 7:332/335 of 4kqxB

query
sites
4kqxB

Y

Y

E

E

Q

Q

D

D

A

V

D

D

L

P

K

K

Y

V

L

I

Q

Q

G

G

K

L

N

K

I

V

A

G

V

I

L

I

G

G

F
x
Y

G
|
G

S
|
S

Q
|
Q

G
|
G

H

H

A

A

H

H

A

A

L

L

N

N

L

L

K

M

E

D

S

S

G

G

L

V

N

D

V

V

C

R

V

V

G

G

L

L

R
|
R

P

E

D

G

S

D

S

S

S

D

C

W

E

K

K

T

A

A

R

E

E

E

N

A

G

G

L

L

E

K

V

V

N

T

T

D

I

M

A

D

D

T

A

A

V
x
A

A
x
E

W

E

A
|
A

D

D

I

V

V

I

M

M

V

V

L
|
L

L
x
V

P
|
P

D
|
D

Q

E

Y

V

Q

Q

K

P

S

K

I
x
V

Y

Y

E

Q

S

E

E

H

I

I

K

A

P

A

N

H

L

L

K

K

E

A

G

G

N
|
N

T

T

L

L

A

A

F

F

A

A

H
|
H

G

G

F

F

N

N

I

I

H

H

Y

Y

N

G

Q

Y

I

I

V

V

P

P

G

E

T

D

V

V

N

N

V

V

I

I

M

M

I

C

A

A

P

P

K
|
K

S

G

P
|
P

G

G

H

H

L

I

V

V

R

R

T

Q

Y

F

T

T

Q

E

G

G

N

S

G

G

V

V

P

P

C

D

L

L

I

A

A

C

V

V

H

Q

Q

Q

D

D

Y

A

T

T

G

G

E

N

A

A

R

W

Q

D

Q

I

A

V

L

L

A

S

W

Y

A

C

K

W

G

G

L

V

G

G

T

A

K

R

A

S

G

G

V

I

I

I

E

K

T

A

T

T

F

F

K

A

N

E

E

E

T

T

E

E

T

E

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

S

L

A

V

E

E

L

L

I

V

K

K

A

A

G

G

F

F

E

E

T

T

L

L

V

T

E

E

A

A

G

G

Y

Y

P

P

E

P

E

E

L

L

A

A

Y

Y

F

F

E

E

C

C

M

Y

H

H

E
|
E

L

M

K

K

L

M

I

I

V

V

D

D

L

L

Y

M

Y

Y

E

E

G

S

G

G

L

I

S

H

R

F

M

M

N
|
N

Y

Y

S
|
S

V
x
I

S
|
S

D
x
N

T

T

A
|
A

E
|
E

Y

Y

G

G

G

E

M

Y

T

Y

R

A

G

G

P

P

R

K

V

V

I

I

T

N

S

E

D

Q

V

S

K

R

A

E

E

A

M

M

K

K

K

E

I

I

L

L

E

K

E

R

V

I

Q

Q

D

D

G

G

R

S

F

F

A

A

K

Q

E

E

F

F

I

V

D

D

E

D

C

C

N

N

A

N

G

G

Y

H

P

K

N

R

M

L

K

L

K

E

L

Q

R

R

E

E

S

A

N

I

A

N

D

T

H

H

P

P

I

I

E

E

K

T

V

T

G

G

A

A

K

Q

L

I

R

R

D

S

M

M

M

F

S

S

W

W

L

I

L

K

K

K

K

E

active site: K132 (= K130), D192 (= D190), E196 (= E194)
binding n-hydroxy-n-isopropyloxamic acid: P134 (= P132), D192 (= D190), E196 (= E194), E232 (= E230), I252 (≠ V250), S253 (= S251), A256 (= A254)
binding magnesium ion: G27 (= G25), Q29 (= Q27), G30 (= G28), A72 (≠ V70), E73 (≠ A71), A75 (= A73), L81 (= L79), N103 (= N101), D192 (= D190), E196 (= E194), N249 (= N247), S251 (= S249), I252 (≠ V250), I252 (≠ V250), S253 (= S251), N254 (≠ D252), E257 (= E255)
binding nicotinamide-adenine-dinucleotide: Y26 (≠ F24), G27 (= G25), S28 (= S26), Q29 (= Q27), R49 (= R47), L81 (= L79), V82 (≠ L80), P83 (= P81), D84 (= D82), V90 (≠ I88), H109 (= H107), P134 (= P132), S251 (= S249), I252 (≠ V250), S253 (= S251)

Sites not aligning to the query:

binding histidine: 333, 334, 335

6jx2B Crystal structure of ketol-acid reductoisomerase from corynebacterium glutamicum (see paper)
58% identity, 98% coverage: 5:327/330 of query aligns to 5:327/327 of 6jx2B

query
sites
6jx2B

Y

Y

E

D

Q

A

D

D

A

A

D

D

L

L

K

S

Y

L

L

I

Q

Q

G

G

K

R

N

K

I

V

A

A

V

I

L

V

G

G

F

Y

G

G

S

S

Q

Q

G

G

H

H

A

A

H

H

A

S

L

Q

N

N

L

L

K

R

E

D

S

S

G

G

L

V

N

E

V

V

C

V

V

I

G

G

L

L

R

R

P

E

D

G

S

S

S

K

S

S

C

A

E

E

K

K

A

A

R

K

E

E

N

A

G

G

L

F

E

E

V

V

N

K

T

T

I

T

A

A

D

E

A

A

V

A

A

A

W

W

A
|
A

D

D

I

V

V

I

M

M

V

L

L

L

L

A

P

P

D

D

Q

T

Y

S

Q

Q

K

A

S

E

I

I

Y

F

E

T

S

N

E

D

I

I

K

E

P

P

N

N

L

L

K
x
N

E

A

G

G

N
x
D

T

A

L

L

A

L

F

F

A

G

H

H

G

G

F

L

N

N

I

I

H

H

Y

F

N

D

Q

L<