SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 642684662 IMG__ChlphaBS1_FD:642684662 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q83E11 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
39% identity, 97% coverage: 6:429/435 of query aligns to 13:421/438 of Q83E11

query
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Q83E11

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K21 (= K15) binding phosphoenolpyruvate
S22 (= S16) binding 3-phosphoshikimate
R26 (= R20) binding 3-phosphoshikimate
NSGT 93:96 (≠ NSGS 94:97) Phosphoenolpyruvate
G95 (= G96) binding phosphoenolpyruvate
T96 (≠ S97) binding phosphoenolpyruvate
R123 (= R124) binding phosphoenolpyruvate
S167 (= S169) binding 3-phosphoshikimate
A168 (= A170) binding 3-phosphoshikimate
Q169 (= Q171) binding 3-phosphoshikimate; binding phosphoenolpyruvate
D315 (= D319) binding 3-phosphoshikimate
K342 (= K346) binding 3-phosphoshikimate
R346 (= R350) binding phosphoenolpyruvate
R387 (= R395) binding phosphoenolpyruvate

3slhD 1.70 angstrom resolution structure of 3-phosphoshikimate 1- carboxyvinyltransferase (aroa) from coxiella burnetii in complex with shikimate-3-phosphate and glyphosate
39% identity, 97% coverage: 6:429/435 of query aligns to 15:423/440 of 3slhD

query
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3slhD

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active site: K23 (= K15), S24 (= S16), D50 (= D42), N95 (= N94), R125 (= R124), D317 (= D319), E345 (= E347), H388 (= H394), R389 (= R395), T415 (≠ V421)
binding glyphosate: K23 (= K15), G97 (= G96), T98 (≠ S97), R125 (= R124), Q171 (= Q171), D317 (= D319), E345 (= E347), R348 (= R350), H388 (= H394), R389 (= R395)
binding shikimate-3-phosphate: S24 (= S16), R28 (= R20), S169 (= S169), Q171 (= Q171), R196 (= R196), D317 (= D319), K344 (= K346)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S24 (= S16), R28 (= R20), T98 (≠ S97), Q171 (= Q171), R196 (= R196), D317 (= D319), K344 (= K346)

4egrA 2.50 angstrom resolution structure of 3-phosphoshikimate 1- carboxyvinyltransferase (aroa) from coxiella burnetii in complex with phosphoenolpyruvate
39% identity, 97% coverage: 6:429/435 of query aligns to 15:419/434 of 4egrA

query
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L

G

G

F

I

V

A

C

A

D

E

E

S

Y

L

A

P

D

D

G

G

F

V

A

I

V

I

T

Q

G

G

R

-

K

-

D

G

V

T

T

L

P

E

T

G

G

G

K

E

V

V

A

-

I

-

D

N

S

S

Y

Y

D

D

H
|
H

R
|
R

I

I

A

A

M

M

S

A

F

F

A

A

I

V

A

A

D

G

R

T

V

L

V

A

P

K

M

G

E

P

L

V

D

R

I

I

S

R

D

N

A

C

K

D

V

N

I

V

G

K

V
x
T

S

S

F

F

P

P

D

N

F

F

F

V

E

E

I

L

active site: K23 (= K15), S24 (= S16), D50 (= D42), N95 (= N94), R125 (= R124), D313 (= D319), E341 (= E347), H384 (= H394), R385 (= R395), T411 (≠ V421)
binding phosphoenolpyruvate: K23 (= K15), G97 (= G96), T98 (≠ S97), R125 (= R124), D313 (= D319), E341 (= E347), R344 (= R350), R385 (= R395)

2pqcA Cp4 epsps liganded with (r)-phosphonate tetrahedral reaction intermediate analog (see paper)
38% identity, 97% coverage: 11:434/435 of query aligns to 19:439/445 of 2pqcA

query
sites
2pqcA

L

I

P

P

P

G

D

D

K
|
K

S
|
S

I

I

A

S

H

H

R
|
R

A

S

A

F

L

M

I

F

G

G

A

G

V

L

A

A

D

S

G

G

T

E

T

T

E

R

I

I

S

T

N

G

F

L

S

L

G

E

G

G

F

E

D
|
D

N

V

Q

I

S

N

T

T

L

G

G

K

V

A

L

M

Q

Q

A

A

C

M

G

G

I

A

A

R

V

I

H

R

Q

K

E

E

K

-

L

-

K

-

G

-

P

-

N

-

G

-

L

-

I

-

S

G

R

D

N

T

V

W

V

I

I

I

H

D

A

G

Q

V

G

G

F

N

W

G

S

G

L

L

S

L

R

A

P

P

E

E

K

A

D

P

L

L

M

D

C

F

N

G

N
|
N

S

A

G

A

S
x
T

T

G

M

C

R

R

M

L

F

T

A

M

G

G

I

L

L

V

A

G

A

V

Q

Y

P

D

F

F

E

D

S

S

R

T

L

F

A

I

G

G

D

D

S

A

S

S

L

L

M

T

K

K

R
|
R

P

P

M

M

Q

G

R

R

V

V

A

L

H

N

P

P

L

L

R

R

L

E

M

M

G

G

A

V

D

Q

V

V

A

K

L

S

S

E

S

D

D

G

N

D

T

R

A

L

P

P

V

V

A

T

I

L

R

R

G

G

T

P

T

K

H

T

L

P

R

T

P

P

I

I

D

T

Y

Y

E

R

L

V

P

P

V

M

P

A

S
|
S

A

A

Q
|
Q

V

V

K

K

S

S

L

A

V

V

A

L

L

L

A

A

A

G

L

L

H

N

A

T

E

P

G

G

E

I

S

T

R

T

V

V

I

I

E

E

T

P

L

I

P

M

S

T

R

R

N

D

H

H

T

T

E

E

L

K

M

M

L

L

-

Q

-

G

-

F

-

G

-

A

G

N

L

L

K

T

V

V

E

E

A

T

L

D

S

A

D

D

G

G

R

V

S

R

A

T

V

I

I

R

I

L

P

E

G

G

R

R

K

G

T

K

V

L

P

T

A

G

K

Q

P

V

F

I

H

D

I

V

P

P

G

G

D

D

P

P

S

S

A

S

A

T

C

A

F

F

I

P

I

L

A

V

L

A

G

A

L

L

S

V

A

P

G

G

S

S

E

D

I

V

V

T

V

I

R

L

D

N

V

V

C

L

L

M

N

N

P

P

T

T

R

R

T

T

G

G

Y

L

M

I

G

L

V

T

L

L

L

Q

G

E

A

M

G

G

A

A

E

D

I

I

S

E

M

V

E

I

N

N

I

P

R

R

F

L

A

A

G

G

E

E

K

D

I

V

G

A

D

D

V

L

L

R

V

V

R

R

H

S

S

S

P

-

S

T

M

L

K

K

P

G

L

V

S

T

I

V

S

P

D

E

P

D

A

R

V

-

V

A

A

P

N

S

S

M

I

I

D
|
D

E

E

I

Y

P

P

M

I

L

L

A

A

V

V

L

A

S

A

A

A

C

F

A

A

T

E

G

G

A

A

F

T

E

V

L

M

Y

N

N

G

A

L

A

E

E

E

L

L

R

R

A

V

K
|
K

E
|
E

S

S

D

D

R
|
R

I

L

S

S

A

A

V

V

V

A

D

N

N

G

L

L

E

K

R

L

I

N

G

G

F

V

V

D

C

C

D

D

E

E

Y

G

A

E

D

T

G

S

F

L

A

V

V

V

T

R

G

G

R

R

K

P

D

D

V

G

T

K

P

G

T

L

G

G

K

N

-

A

-

S

-

G

V

A

A

A

I

V

D

A

S

T

Y

H

D

L

D

D

H
|
H

R
|
R

I

I

A

A

M

M

S

S

F

F

A

L

I

V

A

M

D

G

R

L

V

V

V

S

P

E

M

N

E

P

L

V

D

T

I

V

S

D

D

D

A

A

K

T

V

M

I

I

G

A

V
x
T

S

S

F

F

P

P

D

E

F

F

F

M

E

D

I

L

I

M

E

A

S

G

L

L

A

G

active site: K23 (= K15), S24 (= S16), D50 (= D42), N93 (= N94), R123 (= R124), D321 (= D319), E349 (= E347), H399 (= H394), R400 (= R395), T426 (≠ V421)
binding [3r-[3a,4a,5b(r*)]]-5-(1-carboxy-1-phosphonoethoxy)-4-hydroxy-3-(phosphonooxy)-1-cyclohexene-1-carboxylic acid: K23 (= K15), S24 (= S16), R28 (= R20), T96 (≠ S97), R123 (= R124), S168 (= S169), Q170 (= Q171), D321 (= D319), K348 (= K346), E349 (= E347), R352 (= R350), R400 (= R395)

2pqbA Cp4 epsps liganded with (r)-difluoromethyl tetrahedral intermediate analog (see paper)
38% identity, 97% coverage: 11:434/435 of query aligns to 19:439/445 of 2pqbA

query
sites
2pqbA

L

I

P

P

P

G

D

D

K
|
K

S
|
S

I

I

A

S

H

H

R
|
R

A

S

A

F

L

M

I

F

G

G

A

G

V

L

A

A

D

S

G

G

T

E

T

T

E

R

I

I

S

T

N

G

F

L

S

L

G

E

G

G

F

E

D
|
D

N

V

Q

I

S

N

T

T

L

G

G

K

V

A

L

M

Q

Q

A

A

C

M

G

G

I

A

A

R

V

I

H

R

Q

K

E

E

K

-

L

-

K

-

G

-

P

-

N

-

G

-

L

-

I

-

S

G

R

D

N

T

V

W

V

I

I

I

H

D

A

G

Q

V

G

G

F

N

W

G

S

G

L

L

S

L

R

A

P

P

E

E

K

A

D

P

L

L

M

D

C

F

N

G

N
|
N

S

A

G
x
A

S
x
T

T

G

M

C

R

R

M

L

F

T

A

M

G

G

I

L

L

V

A

G

A

V

Q

Y

P

D

F

F

E

D

S

S

R

T

L

F

A

I

G

G

D

D

S

A

S

S

L

L

M

T

K

K

R
|
R

P

P

M

M

Q

G

R

R

V

V

A

L

H

N

P

P

L

L

R

R

L

E

M

M

G

G

A

V

D

Q

V

V

A

K

L

S

S

E

S

D

D

G

N

D

T

R

A

L

P

P

V

V

A

T

I

L

R

R

G

G

T

P

T

K

H

T

L

P

R

T

P

P

I

I

D

T

Y

Y

E

R

L

V

P

P

V

M

P

A

S
|
S

A

A

Q
|
Q

V

V

K

K

S

S

L

A

V

V

A

L

L

L

A

A

A

G

L

L

H

N

A

T

E

P

G

G

E

I

S

T

R

T

V

V

I

I

E

E

T

P

L

I

P

M

S

T

R

R

N

D

H

H

T

T

E

E

L

K

M

M

L

L

-

Q

-

G

-

F

-

G

-

A

G

N

L

L

K

T

V

V

E

E

A

T

L

D

S

A

D

D

G

G

R

V

S

R

A

T

V

I

I

R

I

L

P

E

G

G

R

R

K

G

T

K

V

L

P

T

A

G

K

Q

P

V

F

I

H

D

I

V

P

P

G

G

D

D

P

P

S

S

A

S

A

T

C

A

F

F

I

P

I

L

A

V

L

A

G

A

L

L

S

V

A

P

G

G

S

S

E

D

I

V

V

T

V

I

R

L

D

N

V

V

C

L

L

M

N

N

P

P

T

T

R

R

T

T

G

G

Y

L

M

I

G

L

V

T

L

L

L

Q

G

E

A

M

G

G

A

A

E

D

I

I

S

E

M

V

E

I

N

N

I

P

R

R

F

L

A

A

G

G

E

E

K

D

I

V

G

A

D

D

V

L

L

R

V

V

R

R

H

S

S

S

P

-

S

T

M

L

K

K

P

G

L

V

S

T

I

V

S

P

D

E

P

D

A

R

V

-

V

A

A

P

N

S

S

M

I

I

D
|
D

E

E

I

Y

P

P

M

I

L

L

A

A

V

V

L

A

S

A

A

A

C

F

A

A

T

E

G

G

A

A

F

T

E

V

L

M

Y

N

N

G

A

L

A

E

E

E

L

L

R

R

A

V

K
|
K

E
|
E

S

S

D

D

R
|
R

I

L

S

S

A

A

V

V

V

A

D

N

N

G

L

L

E

K

R

L

I

N

G

G

F

V

V

D

C

C

D

D

E

E

Y

G

A

E

D

T

G

S

F

L

A

V

V

V

T

R

G

G

R

R

K

P

D

D

V

G

T

K

P

G

T

L

G

G

K

N

-

A

-

S

-

G

V

A

A

A

I

V

D

A

S

T

Y

H

D

L

D

D

H
|
H

R
|
R

I

I

A

A

M

M

S

S

F

F

A

L

I

V

A

M

D

G

R

L

V

V

V

S

P

E

M

N

E

P

L

V

D

T

I

V

S

D

D

D

A

A

K

T

V

M

I

I

G

A

V
x
T

S

S

F

F

P

P

D

E

F

F

F

M

E

D

I

L

I

M

E

A

S

G

L

L

A

G

active site: K23 (= K15), S24 (= S16), D50 (= D42), N93 (= N94), R123 (= R124), D321 (= D319), E349 (= E347), H399 (= H394), R400 (= R395), T426 (≠ V421)
binding (3r,4s,5r)-5-[(1r)-1-carboxy-2,2-difluoro-1-(phosphonooxy)ethoxy]-4-hydroxy-3-(phosphonooxy)cyclohex-1-ene-1-carboxylic acid: K23 (= K15), S24 (= S16), R28 (= R20), A95 (≠ G96), T96 (≠ S97), R123 (= R124), S168 (= S169), Q170 (= Q171), D321 (= D319), K348 (= K346), E349 (= E347), R352 (= R350), R400 (= R395)

2ggaA Cp4 epsp synthase liganded with s3p and glyphosate (see paper)
38% identity, 97% coverage: 11:434/435 of query aligns to 19:439/445 of 2ggaA

query
sites
2ggaA

L

I

P

P

P

G

D

D

K
|
K

S
|
S

I

I

A

S

H

H

R
|
R

A

S

A

F

L

M

I

F

G

G

A

G

V

L

A

A

D

S

G

G

T

E

T

T

E

R

I

I

S

T

N

G

F

L

S

L

G

E

G

G

F

E

D
|
D

N

V

Q

I

S

N

T

T

L

G

G

K

V

A

L

M

Q

Q

A

A

C

M

G

G

I

A

A

R

V

I

H

R

Q

K

E

E

K

-

L

-

K

-

G

-

P

-

N

-

G

-

L

-

I

-

S

G

R

D

N

T

V

W

V

I

I

I

H

D

A

G

Q

V

G

G

F

N

W

G

S

G

L

L

S

L

R

A

P

P

E

E

K

A

D

P

L

L

M

D

C

F

N

G

N
|
N

S
x
A

G
x
A

S
x
T

T

G

M

C

R

R

M

L

F

T

A

M

G

G

I

L

L

V

A

G

A

V

Q

Y

P

D

F

F

E

D

S

S

R

T

L

F

A

I

G

G

D

D

S

A

S

S

L

L

M

T

K

K

R
|
R

P

P

M

M

Q

G

R

R

V

V

A

L

H

N

P

P

L

L

R

R

L

E

M

M

G

G

A

V

D

Q

V

V

A

K

L

S

S

E

S

D

D

G

N

D

T

R

A

L

P

P

V

V

A

T

I

L

R

R

G

G

T

P

T

K

H

T

L

P

R

T

P

P

I

I

D

T

Y

Y

E

R

L

V

P

P

V

M

P

A

S
|
S

A
|
A

Q
|
Q

V

V

K

K

S

S

L

A

V

V

A

L

L

L

A

A

A

G

L

L

H

N

A

T

E

P

G

G

E

I

S

T

R

T

V

V

I

I

E

E

T

P

L

I

P

M

S

T

R
|
R

N

D

H

H

T

T

E

E

L

K

M

M

L

L

-

Q

-

G

-

F

-

G

-

A

G

N

L

L

K

T

V

V

E

E

A

T

L

D

S

A

D

D

G

G

R

V

S

R

A

T

V

I

I

R

I

L

P

E

G

G

R

R

K

G

T

K

V

L

P

T

A

G

K

Q

P

V

F

I

H

D

I

V

P

P

G

G

D

D

P

P

S

S

A

S

A

T

C

A

F

F

I

P

I

L

A

V

L

A

G

A

L

L

S

V

A

P

G

G

S

S

E

D

I

V

V

T

V

I

R

L

D

N

V

V

C

L

L

M

N

N

P

P

T

T

R

R

T

T

G

G

Y

L

M

I

G

L

V

T

L

L

L

Q

G

E

A

M

G

G

A

A

E

D

I

I

S

E

M

V

E

I

N

N

I

P

R

R

F

L

A

A

G

G

E

E

K

D

I

V

G

A

D

D

V

L

L

R

V

V

R

R

H

S

S

S

P

-

S

T

M

L

K

K

P

G

L

V

S

T

I

V

S

P

D

E

P

D

A

R

V

-

V

A

A

P

N

S

S

M

I

I

D
|
D

E

E

I

Y

P

P

M

I

L

L

A

A

V

V

L

A

S

A

A

A

C

F

A

A

T

E

G

G

A

A

F

T

E

V

L

M

Y

N

N

G

A

L

A

E

E

E

L

L

R

R

A

V

K
|
K

E
|
E

S

S

D

D

R
|
R

I

L

S

S

A

A

V

V

V

A

D

N

N

G

L

L

E

K

R

L

I

N

G

G

F

V

V

D

C

C

D

D

E

E

Y

G

A

E

D

T

G

S

F

L

A

V

V

V

T

R

G

G

R

R

K

P

D

D

V

G

T

K

P

G

T

L

G

G

K

N

-

A

-

S

-

G

V

A

A

A

I

V

D

A

S

T

Y

H

D

L

D

D

H
|
H

R
|
R

I

I

A

A

M

M

S

S

F

F

A

L

I

V

A

M

D

G

R

L

V

V

V

S

P

E

M

N

E

P

L

V

D

T

I

V

S

D

D

D

A

A

K

T

V

M

I

I

G

A

V
x
T

S

S

F

F

P

P

D

E

F

F

F

M

E

D

I

L

I

M

E

A

S

G

L

L

A

G

active site: K23 (= K15), S24 (= S16), D50 (= D42), N93 (= N94), R123 (= R124), D321 (= D319), E349 (= E347), H399 (= H394), R400 (= R395), T426 (≠ V421)
binding glyphosate: K23 (= K15), A94 (≠ S95), A95 (≠ G96), T96 (≠ S97), R123 (= R124), D321 (= D319), E349 (= E347), R352 (= R350), R400 (= R395)
binding shikimate-3-phosphate: S24 (= S16), R28 (= R20), S168 (= S169), A169 (= A170), Q170 (= Q171), R195 (= R196), D321 (= D319), K348 (= K346)

2gg6A Cp4 epsp synthase liganded with s3p (see paper)
38% identity, 97% coverage: 11:434/435 of query aligns to 19:439/445 of 2gg6A

query
sites
2gg6A

L

I

P

P

P

G

D

D

K
|
K

S
|
S

I

I

A

S

H

H

R
|
R

A

S

A

F

L

M

I

F

G

G

A

G

V

L

A

A

D

S

G

G

T

E

T

T

E

R

I

I

S

T

N

G

F

L

S

L

G

E

G

G

F

E

D
|
D

N

V

Q

I

S

N

T

T

L

G

G

K

V

A

L

M

Q

Q

A

A

C

M

G

G

I

A

A

R

V

I

H

R

Q

K

E

E

K

-

L

-

K

-

G

-

P

-

N

-

G

-

L

-

I

-

S

G

R

D

N

T

V

W

V

I

I

I

H

D

A

G

Q

V

G

G

F

N

W

G

S

G

L

L

S

L

R

A

P

P

E

E

K

A

D

P

L

L

M

D

C

F

N

G

N
|
N

S

A

G

A

S
x
T

T

G

M

C

R

R

M

L

F

T

A

M

G

G

I

L

L

V

A

G

A

V

Q

Y

P

D

F

F

E

D

S

S

R

T

L

F

A

I

G

G

D

D

S

A

S

S

L

L

M

T

K

K

R
|
R

P

P

M

M

Q

G

R

R

V

V

A

L

H

N

P

P

L

L

R

R

L

E

M

M

G

G

A

V

D

Q

V

V

A

K

L

S

S

E

S

D

D

G

N

D

T

R

A

L

P

P

V

V

A

T

I

L

R

R

G

G

T

P

T

K

H

T

L

P

R

T

P

P

I

I

D

T

Y

Y

E

R

L

V

P

P

V

M

P

A

S
|
S

A

A

Q
|
Q

V

V

K

K

S

S

L

A

V

V

A

L

L

L

A

A

A

G

L

L

H

N

A

T

E

P

G

G

E

I

S

T

R

T

V

V

I

I

E

E

T

P

L

I

P

M

S

T

R

R

N

D

H

H

T

T

E

E

L

K

M

M

L

L

-

Q

-

G

-

F

-

G

-

A

G

N

L

L

K

T

V

V

E

E

A

T

L

D

S

A

D

D

G

G

R

V

S

R

A

T

V

I

I

R

I

L

P

E

G

G

R

R

K

G

T

K

V

L

P

T

A

G

K

Q

P

V

F

I

H

D

I

V

P

P

G

G

D

D

P

P

S

S

A

S

A

T

C

A

F

F

I

P

I

L

A

V

L

A

G

A

L

L

S

V

A

P

G

G

S

S

E

D

I

V

V

T

V

I

R

L

D

N

V

V

C

L

L

M

N

N

P

P

T

T

R

R

T

T

G

G

Y

L

M

I

G

L

V

T

L

L

L

Q

G

E

A

M

G

G

A

A

E

D

I

I

S

E

M

V

E

I

N

N

I

P

R

R

F

L

A

A

G

G

E

E

K

D

I

V

G

A

D

D

V

L

L

R

V

V

R

R

H

S

S

S

P

-

S

T

M

L

K

K

P

G

L

V

S

T

I

V

S

P

D

E

P

D

A

R

V

-

V

A

A

P

N

S

S

M

I

I

D
|
D

E

E

I

Y

P

P

M

I

L

L

A

A

V

V

L

A

S

A

A

A

C

F

A

A

T

E

G

G

A

A

F

T

E

V

L

M

Y

N

N

G

A

L

A

E

E

E

L

L

R

R

A

V

K
|
K

E
|
E

S

S

D

D

R

R

I

L

S

S

A

A

V

V

V

A

D

N

N

G

L

L

E

K

R

L

I

N

G

G

F

V

V

D

C

C

D

D

E

E

Y

G

A

E

D

T

G

S

F

L

A

V

V

V

T

R

G

G

R

R

K

P

D

D

V

G

T

K

P

G

T

L

G

G

K

N

-

A

-

S

-

G

V

A

A

A

I

V

D

A

S

T

Y

H

D

L

D

D

H
|
H

R
|
R

I

I

A

A

M

M

S

S

F

F

A

L

I

V

A

M

D

G

R

L

V

V

V

S

P

E

M

N

E

P

L

V

D

T

I

V

S

D

D

D

A

A

K

T

V

M

I

I

G

A

V
x
T

S

S

F

F

P

P

D

E

F

F

F

M

E

D

I

L

I

M

E

A

S

G

L

L

A

G

active site: K23 (= K15), S24 (= S16), D50 (= D42), N93 (= N94), R123 (= R124), D321 (= D319), E349 (= E347), H399 (= H394), R400 (= R395), T426 (≠ V421)
binding shikimate-3-phosphate: S24 (= S16), R28 (= R20), T96 (≠ S97), S168 (= S169), Q170 (= Q171), D321 (= D319), K348 (= K346)

Q9R4E4 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; CP4 EPSP synthase; EC 2.5.1.19 from Agrobacterium sp. (strain CP4) (see paper)
38% identity, 97% coverage: 11:434/435 of query aligns to 24:444/455 of Q9R4E4

query
sites
Q9R4E4

L

I

P

P

P

G

D

D

K

K

S
|
S

I

I

A

S

H

H

R
|
R

A

S

A

F

L

M

I

F

G

G

A

G

V

L

A

A

D

S

G

G

T

E

T

T

E

R

I

I

S

T

N

G

F

L

S

L

G

E

G

G

F

E

D

D

N

V

Q

I

S

N

T

T

L

G

G

K

V

A

L

M

Q

Q

A

A

C

M

G

G

I

A

A

R

V

I

H

R

Q

K

E

E

K

-

L

-

K

-

G

-

P

-

N

-

G

-

L

-

I

-

S

G

R

D

N

T

V

W

V

I

I

I

H

D

A

G

Q

V

G

G

F

N

W

G

S

G

L

L

S

L

R

A

P

P

E

E

K

A

D

P

L

L

M

D

C

F

N

G

N

N

S

A

G
x
A

S

T

T

G

M

C

R

R

M

L

F

T

A

M

G

G

I

L

L

V

A

G

A

V

Q

Y

P

D

F

F

E

D

S

S

R

T

L

F

A

I

G

G

D

D

S

A

S

S

L

L

M

T

K

K

R

R

P

P

M

M

Q

G

R

R

V

V

A

L

H

N

P

P

L

L

R

R

L

E

M

M

G

G

A

V

D

Q

V

V

A

K

L

S

S

E

S

D

D

G

N

D

T

R

A

L

P

P

V

V

A

T

I

L

R

R

G

G

T

P

T

K

H

T

L

P

R

T

P

P

I

I

D

T

Y

Y

E

R

L

V

P

P

V

M

P

A

S
|
S

A
|
A

Q
|
Q

V

V

K

K

S

S

L

A

V

V

A

L

L

L

A

A

A

G

L

L

H

N

A

T

E

P

G

G

E

I

S

T

R

T

V

V

I

I

E

E

T

P

L

I

P

M

S

T

R

R

N

D

H

H

T

T

E

E

L

K

M

M

L

L

-

Q

-

G

-

F

-

G

-

A

G

N

L

L

K

T

V

V

E

E

A

T

L

D

S

A

D

D

G

G

R

V

S

R

A

T

V

I

I

R

I

L

P

E

G

G

R

R

K

G

T

K

V

L

P

T

A

G

K

Q

P

V

F

I

H

D

I

V

P

P

G

G

D

D

P

P

S

S

A

S

A

T

C

A

F

F

I

P

I

L

A

V

L

A

G

A

L

L

S

V

A

P

G

G

S

S

E

D

I

V

V

T

V

I

R

L

D

N

V

V

C

L

L

M

N

N

P

P

T

T

R

R

T

T

G

G

Y

L

M

I

G

L

V

T

L

L

L

Q

G

E

A

M

G

G

A

A

E

D

I

I

S

E

M

V

E

I

N

N

I

P

R

R

F

L

A

A

G

G

E

E

K

D

I

V

G

A

D

D

V

L

L

R

V

V

R

R

H

S

S

S

P

-

S

T

M

L

K

K

P

G

L

V

S

T

I

V

S

P

D

E

P

D

A

R

V

-

V

A

A

P

N

S

S

M

I

I

D
|
D

E

E

I

Y

P

P

M

I

L

L

A

A

V

V

L

A

S

A

A

A

C

F

A

A

T

E

G

G

A

A

F

T

E

V

L

M

Y

N

N

G

A

L

A

E

E

E

L

L

R

R

A

V

K
|
K

E

E

S

S

D

D

R

R

I

L

S

S

A

A

V

V

V

A

D

N

N

G

L

L

E

K

R

L

I

N

G

G

F

V

V

D

C

C

D

D

E

E

Y

G

A

E

D

T

G

S

F

L

A

V

V

V

T

R

G

G

R

R

K

P

D

D

V

G

T

K

P

G

T

L

G

G

K

N

-

A

-

S

-

G

V

A

A

A

I

V

D

A

S

T

Y

H

D

L

D

D

H

H

R

R

I

I

A

A

M

M

S

S

F

F

A

L

I

V

A

M

D

G

R

L

V

V

V

S

P

E

M

N

E

P

L

V

D

T

I

V

S

D

D

D

A

A

K

T

V

M

I

I

G

A

V

T

S

S

F

F

P

P

D

E

F

F

F

M

E

D

I

L

I

M

E

A

S

G

L

L

A

G

S29 (= S16) binding 3-phosphoshikimate
R33 (= R20) binding 3-phosphoshikimate
A100 (≠ G96) mutation to G: Confers sensitivity to glyphosate allowing glyphosate to bind in its extended, inhibitory conformation.
S173 (= S169) binding 3-phosphoshikimate
A174 (= A170) binding 3-phosphoshikimate
Q175 (= Q171) binding 3-phosphoshikimate
D326 (= D319) binding 3-phosphoshikimate
K353 (= K346) binding 3-phosphoshikimate

Q9S400 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) (see paper)
35% identity, 97% coverage: 11:433/435 of query aligns to 16:424/427 of Q9S400

query
sites
Q9S400

L

V

P

P

P

G

D

D

K

K

S
|
S

I

I

A

S

H

H

R
|
R

A

S

A

I

L

I

I

F

G

G

A

S

V

L

A

A

D

E

G

G

T

E

T

T

E

K

I

V

S

Y

N

D

F

I

S

L

G

R

G

G

F

E

D

D

N

V

Q

L

S

S

T

T

L

M

G

Q

V

V

L

F

Q

R

A

D

C

L

G

G

I

V

A

E

V

I

H

E

Q

D

E

K

K

-

L

-

K

-

G

-

P

-

N

D

G

G

L

V

I

I

S

T

R

-

N

-

V

-

I

I

H

Q

A

G

Q

V

G

G

F

M

W

A

S

G

L

L

S

K

R

A

P

P

E

Q

K

N

D

A

L

L

M

N

C

M

N

G

N

N

S

S

G

G

S

T

T

S

M

I

R

R

M

L

F

I

A

S

G

G

I

V

L

L

A

A

A

G

Q

A

P

D

F

F

E

E

S

V

R

E

L

M

A

F

G

G

D

D

S

D

S

S

L

L

M

S

K

K

R

R

P

P

M

M

Q

D

R

R

V

V

A

T

H

L

P

P

L

L

R

K

L

K

M

M

G

G

A

V

D

S

V

I

A

S

L

G

S

Q

S

T

D

E

-

R

N

D

T

L

A

P

P

P

V

L

A

R

I

L

R

K

G

G

T

T

T

K

H

N

L

L

R

R

P

P

I

I

D

H

Y

Y

E

E

L

L

P

P

V

I

P

A

S
|
S

A
|
A

Q
|
Q

V

V

K

K

S

S

L

A

V

L

A

M

L

F

A

A

L

L

H

Q

A

A

E

K

G

G

E

E

S

S

R

V

V

I

I

I

E

E

T

K

L

E

P

Y

S

T

R

R

N

N

H

H

T

T

E

E

L

D

M

M

L

L

G

K

L

Q

K

F

V

G

E

G

A

H

L

L

S

S

D

V

G

D

R

G

S

K

A

K

V

I

I

T

I

V

P

Q

G

G

R

P

K

Q

T

K

V

L

P

T

A

G

K

Q

P

K

F

V

H

V

I

V

P

P

G

G

D

D

P

I

S

S

A

S

A

A

C

A

F

F

I

W

I

L

A

V

L

A

G

G

L

L

L

I

S

A

A

P

G

N

S

S

E

R

I

L

V

V

V

L

R

Q

D

N

V

V

C

G

L

I

N

N

P

E

T

T

R

R

T

T

G

G

Y

I

M

I

G

D

V

V

L

I

L

R

G

A

A

M

G

G

A

G

E

K

I

L

S

E

M

I

E

T

N

E

I

I

R

D

F

P

A

V

G

A

G

K

E

S

K

A

I

-

G

-

D

-

V

T

L

L

V

I

R

V

H

E

S

S

P

S

S

D

M

L

K

K

P

G

L

T

S

E

I

I

S

G

D

G

P

-

A

A

V

L

V

I

A

P

N

R

S

L

I

I

D
|
D

E

E

I

L

P

P

M

I

L

I

A

A

V

L

L

L

S

A

A

T

C

Q

A

A

T

Q

G

G

A

V

F

T

E

V

L

I

Y

K

N

D

A

A

A

E

E

E

L

L

R

K

A

V

K
|
K

E

E

S

T

D

D

R

R

I

I

S

Q

A

V

V

V

V

A

D

D

N

A

L

L

E

N

R

S

I

M

G

G

F

A

V

D

C

I

D

T

E

P

Y

T

A

A

D

D

G

G

F

M

A

I

V

I

T

K

G

G

R

K

K

-

D

-

V

-

T

S

P

A

T

L

G

H

K

G

V

A

A

R

I

V

D

N

S

T

Y

F

D

G

D

D

H

H

R

R

I

I

A

G

M

M

S

M

F

T

A

A

I

I

A

A

D

A

R

L

V

L

V

V

P

A

-

D

M

G

E

E

L

V

D

E

I

L

S

D

D

R

A

A

K

E

V

A

I

I

G

N

V

T

S

S

F

Y

P

P

D

S

F

F

E

D

I

D

I

L

E

E

S

S

L

L

S21 (= S16) binding 3-phosphoshikimate
R25 (= R20) binding 3-phosphoshikimate
S166 (= S169) binding 3-phosphoshikimate
A167 (= A170) binding 3-phosphoshikimate
Q168 (= Q171) binding 3-phosphoshikimate
D312 (= D319) binding 3-phosphoshikimate
K339 (= K346) binding 3-phosphoshikimate

1rf6A Structural studies of streptococcus pneumoniae epsp synthase in s3p- glp bound state (see paper)
35% identity, 97% coverage: 11:433/435 of query aligns to 16:424/427 of 1rf6A

query
sites
1rf6A

L

V

P

P

P

G

D

D

K
|
K

S
|
S

I

I

A

S

H

H

R
|
R

A

S

A

I

L

I

I

F

G

G

A

S

V

L

A

A

D

E

G

G

T

E

T

T

E

K

I

V

S

Y

N

D

F

I

S

L

G

R

G

G

F

E

D
|
D

N

V

Q

L

S

S

T

T

L

M

G

Q

V

V

L

F

Q

R

A

D

C

L

G

G

I

V

A

E

V

I

H

E

Q

D

E

K

K

-

L

-

K

-

G

-

P

-

N

D

G

G

L

V

I

I

S

T

R

-

N

-

V

-

I

V

H

Q

A

G

Q

V

G

G

F

M

W

A

S

G

L

L

S

K

R

A

P

P

E

Q

K

N

D

A

L

L

M

N

C

M

N

G

N
|
N

S

S

G
|
G

S
x
T

T

S

M

I

R

R

M

L

F

I

A

S

G

G

I

V

L

L

A

A

A

G

Q

A

P

D

F

F

E

E

S

V

R

E

L

M

A

F

G

G

D

D

S
x
D

S

S

L

L

M

S

K

K

R
|
R

P

P

M

M

Q

D

R

R

V

V

A

T

H

L

P

P

L

L

R

K

L

K

M

M

G

G

A

V

D

S

V

I

A

S

L

G

S

Q

S

T

D

E

-

R

N

D

T

L

A

P

P

P

V

L

A

R

I

L

R

K

G

G

T

T

T

K

H

N

L

L

R

R

P

P

I

I

D

H

Y

Y

E

E

L

L

P

P

V

I

P

A

S
|
S

A

A

Q
|
Q

V

V

K

K

S

S

L

A

V

L

A

M

L

F

A

A

L

L

H

Q

A

A

E

K

G

G

E

E

S

S

R

V

V

I

I

I

E

E

T

K

L

E

P

Y

S

T

R
|
R

N

N

H

H

T

T

E

E

L

D

M

M

L

L

G

Q

L

Q

K

F

V

G

E

G

A

H

L

L

S

S

D

V

G

D

R

G

S

K

A

K

V

I

I

T

I

V

P

Q

G

G

R

P

K

Q

T

K

V

L

P

T

A

G

K

Q

P

K

F

V

H

V

I

V

P

P

G

G

D

D

P

I

S

S

A

S

A

A

C

A

F

F

I

W

I

L

A

V

L

A

G

G

L

L

L

I

S

A

A

P

G

N

S

S

E

R

I

L

V

V

V

L

R

Q

D

N

V

V

C

G

L

I

N

N

P

E

T

T

R

R

T

T

G

G

Y

I

M

I

G

D

V

V

L

I

L

R

G

A

A

M

G

G

A

G

E

K

I

L

S

E

M

I

E

T

N

E

I

I

R

D

F

P

A

V

G

A

G

K

E

S

K

A

I

-

G

-

D

-

V

T

L

L

V

I

R

V

H

E

S

S

P

S

S

D

M

L

K

K

P

G

L

T

S

E

I

I

S

C

D

G

P

-

A

A

V

L

V

I

A

P

N

R

S

L

I
|
I

D
|
D

E

E

I

L

P

P

M

I

L

I

A

A

V

L

L

L

S

A

A

T

C

Q

A

A

T

Q

G

G

A

V

F

T

E

V

L

I

Y

K

N

D

A

A

A

E

E

E

L

L

R

K

A

V

K
|
K

E
|
E

S

T

D

D

R
|
R

I

I

S

Q

A

V

V

V

V

A

D

D

N

A

L

L

E

N

R

S

I

M

G

G

F

A

V

D

C

I

D

T

E

P

Y

T

A

A

D

D

G

G

F

M

A

I

V

I

T

K

G

G

R

K

K

-

D

-

V

-

T

S

P

A

T

L

G

H

K

G

V

A

A

R

I

V

D

N

S

T

Y

F

D

G

D

D

H
|
H

R
|
R

I

I

A

G

M

M

S

M

F

T

A

A

I

I

A

A

D

A

R

L

V

L

V

V

P

A

-

D

M

G

E

E

L

V

D

E

I

L

S

D

D

R

A

A

K

E

V

A

I

I

G

N

V
x
T

S

S

F

Y

P

P

D

S

F

F

E

D

I

D

I

L

E

E

S

S

L

L

active site: K20 (= K15), S21 (= S16), D47 (= D42), N90 (= N94), D115 (≠ S119), R120 (= R124), D312 (= D319), E340 (= E347), H384 (= H394), R385 (= R395), T412 (≠ V421)
binding glyphosate: K20 (= K15), G92 (= G96), T93 (≠ S97), R120 (= R124), Q168 (= Q171), D312 (= D319), E340 (= E347), R343 (= R350), H384 (= H394), R385 (= R395)
binding shikimate-3-phosphate: S21 (= S16), R25 (= R20), S166 (= S169), Q168 (= Q171), R193 (= R196), I311 (= I318), D312 (= D319), K339 (= K346)

1rf4A Structural studies of streptococcus pneumoniae epsp synthase, tetrahedral intermediate bound state (see paper)
35% identity, 97% coverage: 11:433/435 of query aligns to 16:424/427 of 1rf4A

query
sites
1rf4A

L

V

P

P

P

G

D

D

K
|
K

S
|
S

I

I

A

S

H

H

R
|
R

A

S

A

I

L

I

I

F

G

G

A

S

V

L

A

A

D

E

G

G

T

E

T

T

E

K

I

V

S

Y

N

D

F

I

S

L

G

R

G

G

F

E

D
|
D

N

V

Q

L

S

S

T

T

L

M

G

Q

V

V

L

F

Q

R

A

D

C

L

G

G

I

V

A

E

V

I

H

E

Q

D

E

K

K

-

L

-

K

-

G

-

P

-

N

D

G

G

L

V

I

I

S

T

R

-

N

-

V

-

I

V

H

Q

A

G

Q

V

G

G

F

M

W

A

S

G

L

L

S

K

R

A

P

P

E

Q

K

N

D

A

L

L

M

N

C

M

N

G

N
|
N

S

S

G
|
G

S
x
T

T

S

M

I

R

R

M

L

F

I

A

S

G

G

I

V

L

L

A

A

A

G

Q

A

P

D

F

F

E

E

S

V

R

E

L

M

A

F

G

G

D

D

S
x
D

S

S

L

L

M

S

K

K

R
|
R