SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 642684670 IMG__ChlphaBS1_FD:642684670 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P23247 Aspartate-semialdehyde dehydrogenase 2; ASA dehydrogenase 2; ASADH 2; Aspartate-beta-semialdehyde dehydrogenase 2; EC 1.2.1.11 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (see paper)
52% identity, 95% coverage: 16:346/349 of query aligns to 3:333/337 of P23247

query
sites
P23247

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C132 (= C147) active site, Acyl-thioester intermediate

2r00C Crystal structure of aspartate semialdehyde dehydrogenase ii complexed with asa from vibrio cholerae (see paper)
52% identity, 95% coverage: 16:346/349 of query aligns to 2:332/336 of 2r00C

query
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2r00C

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active site: C131 (= C147), Q158 (= Q174), R237 (= R249), H244 (= H256)
binding 2,2'-oxydiacetic acid: V15 (= V29), N95 (= N109), C131 (= C147), N315 (= N329)

Q04797 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Bacillus subtilis (strain 168) (see paper)
48% identity, 93% coverage: 20:345/349 of query aligns to 7:340/346 of Q04797

query
sites
Q04797

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S98 (= S111) modified: Phosphoserine
Y146 (= Y163) modified: Phosphotyrosine

4r54A Complex crystal structure of sp-aspartate-semialdehyde-dehydrogenase with 3-carboxy-ethyl-phthalic acid (see paper)
45% identity, 94% coverage: 18:345/349 of query aligns to 2:340/357 of 4r54A

query
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4r54A

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D

E

D

V

F

A

D

P

V

I

D

E

E

E

L

V

R

K

E

A

M

A

L

I

A

A

G

A

T

F

P

P

G

G

I

A

I

V

V

L

Q

E

D

D

P

V

S

A

S

H

G

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

T

N

S

A

Y

V

E

G

K

S

D

R

E

D

V

T

F

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

F

L

W

D

Q

A

P

E

K

K

T

G

V

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

L

L

R

L

K

K

G
|
G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

K

T

L

L

binding 3-(2-carboxyethyl)benzene-1,2-dicarboxylic acid: G72 (= G88), S73 (≠ A89), T94 (≠ S110), S95 (= S111), R98 (= R114), K222 (= K227)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G24), T10 (= T26), G11 (= G27), A12 (≠ L28), V13 (= V29), A35 (= A51), S36 (= S52), R38 (= R54), S39 (= S55), T56 (≠ P72), S70 (= S86), A71 (= A87), G72 (= G88), T75 (≠ A91), N93 (= N109), T94 (≠ S110), N126 (= N146), C127 (= C147), G160 (= G180), G328 (= G333)

4r41A Complex crystal structure of 4-nitro-2-phosphono-benzoic acid with sp- aspartate-semialdehyde dehydrogenase and nicotinamide-dinucleotide (see paper)
45% identity, 94% coverage: 18:345/349 of query aligns to 2:340/357 of 4r41A

query
sites
4r41A

Y

Y

S

T

I

V

A

A

I

V

L

V

G
|
G

A

A

T
|
T

G
|
G

L
x
A

V

V

G

G

R

A

T

Q

M

M

L

I

Q

K

V

M

L

L

D

E

E

E

R

S

D

T

F

L

P

P

V

I

R

D

E

K

I

I

L

R

P

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

R

A

G

G

R

K

E

S

I

L

V

K

F

F

R

K

G

D

K

Q

S

D

F

I

R

T

T

I

D

E

V

E

P
x
T

S

T

A

E

E

T

V

A

F

F

S

E

R

G

A

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

A
x
S

T

S

A

T

S

S

R

A

T

K

W

Y

A

A

S

P

V

Y

A

A

A

V

E

K

A

A

G

G

A

V

V

V

I

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

A

A

I

L

F

-

D

-

V

-

Q

-

G

D

K

A

P

H

K

N

G

G

V

I

I

I

A

A

N

C

P

P

N

N

C

C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

R

K

Y

W

R

G

V

L

R

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q

Q

S

A

V

V

T

S

G

G

K

A

G
|
G

-
x
M

-
x
G

-

A

-

I

-

L

-

E

-

T

-

Q

K

R

Q

E

G

L

R

R

D

E

A

V

L

L

E

N

D

D

E

G

L

V

-

K

-

P

-

C

-

D

-

L

-

H

A

A

G

E

K

I

M

L

P

P

E

S

S

G

F

G

V

D

H

K

G

K

H

H

Q

Y

-

P

I

I

A

A

F

F

N

N

A

A

V

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

D

D

N

N

G

D

Y

Y

T

T

K

Y

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

R

K

K

K

I

I

M

M

C

E

D

D

E

D

S

S

L

I

S

A

V

V

S

S

P

A

T

T

T

C

V

V

R

R

I

I

P

P

V

V

Y

L

G

S

G

A

H

H

G

S

E

E

S

S

M

V

N

Y

I

I

E

E

F

T

E

K

D

E

D

V

F

A

D

P

V

I

D

E

E

E

L

V

R

K

E

A

M

A

L

I

A

A

G

A

T

F

P

P

G

G

I

A

I

V

V

L

Q

E

D

D

P

V

S

A

S

H

G

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

T

N

S

A

Y

V

E

G

K

S

D

R

E

D

V

T

F

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

F

L

W

D

Q

A

P

E

K

K

T

G

V

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

L

L

R

L

K

K

G

G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

K

T

L

L

binding 4-nitro-2-phosphonobenzoic acid: S70 (= S86), G72 (= G88), S73 (≠ A89), N93 (= N109), T94 (≠ S110), S95 (= S111), R98 (= R114), K222 (= K227)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G24), T10 (= T26), G11 (= G27), A12 (≠ L28), A35 (= A51), S36 (= S52), R38 (= R54), S39 (= S55), T56 (≠ P72), A71 (= A87), G160 (= G180), M161 (vs. gap), G162 (vs. gap)

4r3nA Crystal structure of the ternary complex of sp-asadh with NADP and 1, 2,3-benzenetricarboxylic acid (see paper)
45% identity, 94% coverage: 18:345/349 of query aligns to 2:340/357 of 4r3nA

query
sites
4r3nA

Y

Y

S

T

I

V

A

A

I

V

L

V

G
|
G

A

A

T
|
T

G
|
G

L
x
A

V
|
V

G

G

R

A

T

Q

M

M

L

I

Q

K

V

M

L

L

D

E

E

E

R

S

D

T

F

L

P

P

V

I

R

D

E

K

I

I

L

R

P

Y

L

L

A
|
A

S
|
S

E

A

R

R

S
|
S

R

A

G

G

R

K

E

S

I

L

V

K

F

F

R

K

G

D

K

Q

S

D

F

I

R

T

T

I

D

E

V

E

P
x
T

S

T

A

E

E

T

V

A

F

F

S

E

R

G

A

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

A
x
S

T

S

A

T

S

S

R

A

T

K

W

Y

A

A

S

P

V

Y

A

A

A

V

E

K

A

A

G

G

A

V

V

V

I

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

A

A

I

L

F

-

D

-

V

-

Q

-

G

D

K

A

P

H

K

N

G

G

V

I

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

R

K

Y

W

R

G

V

L

R

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

S

A

V

V

T

S

G

G

K

A

G
|
G

-
x
M

-

G

-

A

-

I

-

L

-

E

-

T

-

Q

K

R

Q

E

G

L

R

R

D

E

A

V

L

L

E

N

D

D

E

G

L

V

-

K

-

P

-

C

-

D

-

L

-

H

A

A

G

E

K

I

M

L

P

P

E

S

S

G

F

G

V

D

H

K

G

K

H

H

Q

Y

-

P

I

I

A

A

F

F

N

N

A

A

V

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

D

D

N

N

G

D

Y

Y

T

T

K

Y

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

R

K

K

K

I

I

M

M

C

E

D

D

E

D

S

S

L

I

S

A

V

V

S

S

P

A

T

T

T

C

V

V

R
|
R

I

I

P

P

V

V

Y

L

G

S

G

A

H
|
H

G

S

E

E

S

S

M

V

N

Y

I

I

E

E

F

T

E

K

D

E

D

V

F

A

D

P

V

I

D

E

E

E

L

V

R

K

E

A

M

A

L

I

A

A

G

A

T

F

P

P

G

G

I

A

I

V

V

L

Q

E

D

D

P

V

S

A

S

H

G

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

T

N

S

A

Y

V

E

G

K

S

D

R

E

D

V

T

F

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

F

L

W

D

Q

A

P

E

K

K

T

G

V

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

L

L

R

L

K

K

G
|
G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

K

T

L

L

active site: C127 (= C147), Q154 (= Q174), R244 (= R249), H251 (= H256)
binding benzene-1,2,3-tricarboxylic acid: S73 (≠ A89), T94 (≠ S110), S95 (= S111), R98 (= R114), N126 (= N146), K222 (= K227)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G24), T10 (= T26), G11 (= G27), A12 (≠ L28), V13 (= V29), A35 (= A51), S36 (= S52), S39 (= S55), T56 (≠ P72), S70 (= S86), A71 (= A87), G72 (= G88), N93 (= N109), T94 (≠ S110), N126 (= N146), C127 (= C147), G160 (= G180), M161 (vs. gap), G328 (= G333)

3q1lA Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with cysteamine bound covalently to cys 128 (see paper)
45% identity, 94% coverage: 18:345/349 of query aligns to 2:340/357 of 3q1lA

query
sites
3q1lA

Y

Y

S

T

I

V

A

A

I

V

L

V

G

G

A

A

T

T

G

G

L

A

V

V

G

G

R

A

T

Q

M

M

L

I

Q

K

V

M

L

L

D

E

E

E

R

S

D

T

F

L

P

P

V

I

R

D

E

K

I

I

L

R

P

Y

L

L

A

A

S

S

E

A

R

R

S

S

R

A

G

G

R

K

E

S

I

L

V

K

F

F

R

K

G

D

K

Q

S

D

F

I

R

T

T

I

D

E

V

E

P

T

S

T

A

E

E

T

V

A

F

F

S

E

R

G

A

V

D

D

I

I

A

A

L

L

F

F

S

S

A

A

G

G

A

S

T

S

A

T

S

S

R

A

T

K

W

Y

A

A

S

P

V

Y

A

A

A

V

E

K

A

A

G

G

A

V

V

V

I

V

D

D

N

N

S

T

S

S

A

Y

F

F

R

R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

A

A

I

L

F

-

D

-

V

-

Q

-

G

D

K

A

P

H

K

N

G

G

V

I

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

R

K

Y

W

R

G

V

L

R

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

S

A

V

V

T

S

G
|
G

K

A

G

G

-

M

-

G

-

A

-

I

-

L

-

E

-

T

-

Q

K

R

Q

E

G

L

R

R

D

E

A

V

L

L

E

N

D

D

E

G

L

V

-

K

-

P

-

C

-

D

-

L

-

H

A

A

G

E

K

I

M

L

P

P

E

S

S

G

F

G

V

D

H

K

G

K

H

H

Q

Y

-

P

I

I

A

A

F

F

N

N

A

A

V

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

D

D

N

N

G

D

Y

Y

T

T

K

Y

E
|
E

E

E

M

M

K

K

M

M

V

T

N

K

E

E

T

T

R

K

K

K

I

I

M

M

C

E

D

D

E

D

S

S

L

I

S

A

V

V

S

S

P

A

T

T

T

C

V

V

R
|
R

I

I

P

P

V

V

Y

L

G

S

G

A

H
|
H

G

S

E

E

S

S

M

V

N

Y

I

I

E

E

F

T

E

K

D

E

D

V

F

A

D

P

V

I

D

E

E

E

L

V

R

K

E

A

M

A

L

I

A

A

G

A

T

F

P

P

G

G

I

A

I

V

V

L

Q

E

D

D

P

V

S

A

S

H

G

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

T

N

S

A

Y

V

E

G

K

S

D

R

E

D

V

T

F

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

F

L

W

D

Q

A

P

E

K

K

T

G

V

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

L

L

R

L

K

K

G

G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

K

T

L

L

active site: C127 (= C147), Q154 (= Q174), R244 (= R249), H251 (= H256)
binding 2-amino-ethanethiol: N126 (= N146), C127 (= C147), G158 (= G178), E219 (= E224)

3pwsA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
45% identity, 94% coverage: 18:345/349 of query aligns to 2:340/357 of 3pwsA

query
sites
3pwsA

Y

Y

S

T

I

V

A

A

I

V

L

V

G
|
G

A

A

T
|
T

G
|
G

L
x
A

V

V

G

G

R

A

T

Q

M

M

L

I

Q

K

V

M

L

L

D

E

E

E

R

S

D

T

F

L

P

P

V

I

R

D

E

K

I

I

L

R

P

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

R

A

G

G

R

K

E

S

I

L

V

K

F

F

R

K

G

D

K

Q

S

D

F

I

R

T

T

I

D

E

V

E

P
x
T

S

T

A

E

E

T

V

A

F

F

S

E

R

G

A

V

D

D

I

I

A

A

L

L

F

F

S

S

A
|
A

G

G

A

S

T

S

A
x
T

S

S

R

A

T

K

W

Y

A

A

S

P

V

Y

A

A

A

V

E

K

A

A

G

G

A

V

V

V

I

V

D

D

N

N

S

T

S

S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

A

A

I

L

F

-

D

-

V

-

Q

-

G

D

K

A

P

H

K

N

G

G

V

I

I

I

A

A

N

C

P

P

N
|
N

C

C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

R

K

Y

W

R

G

V

L

R

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q

Q

S

A

V

V

T

S

G
|
G

K

A

G
|
G

-
x
M

-

G

-

A

-

I

-

L

-

E

-

T

-

Q

K

R

Q

E

G

L

R

R

D

E

A

V

L

L

E

N

D

D

E

G

L

V

-

K

-

P

-

C

-

D

-

L

-

H

A

A

G

E

K

I

M

L

P

P

E

S

S

G

F

G

V

D

H

K

G

K

H

H

Q

Y

-

P

I

I

A

A

F

F

N

N

A

A

V

L

P

P

H

Q

I
|
I

D

D

V

V

F

F

L

T

D

D

N

N

G

D

Y

Y

T

T

K

Y

E
|
E

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

R

K

K

K

I

I

M

M

C

E

D

D

E

D

S

S

L

I

S

A

V

V

S

S

P

A

T

T

T

C

V

V

R
|
R

I

I

P

P

V

V

Y

L

G

S

G

A

H

H

G

S

E

E

S

S

M

V

N

Y

I

I

E

E

F

T

E

K

D

E

D

V

F

A

D

P

V

I

D

E

E

E

L

V

R

K

E

A

M

A

L

I

A

A

G

A

T

F

P

P

G

G

I

A

I

V

V

L

Q

E

D

D

P

V

S

A

S

H

G

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

T

N

S

A

Y

V

E

G

K

S

D

R

E

D

V

T

F

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

F

L

W

D

Q

A

P

E

K

K

T

G

V

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

L

L

R

L

K

K

G

G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

K

T

L

L

binding (2R)-2-aminohexanedioic acid: R98 (= R114), N126 (= N146), G158 (= G178), I208 (= I213), E219 (= E224), K222 (= K227), R244 (= R249)
binding adenosine-2'-5'-diphosphate: G8 (= G24), T10 (= T26), G11 (= G27), A12 (≠ L28), A35 (= A51), S36 (= S52), R38 (= R54), S39 (= S55), T56 (≠ P72), A71 (= A87), T75 (≠ A91), G160 (= G180), M161 (vs. gap)

3pwkA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
45% identity, 94% coverage: 18:345/349 of query aligns to 2:340/357 of 3pwkA

query
sites
3pwkA

Y

Y

S

T

I

V

A

A

I

V

L

V

G
|
G

A

A

T
|
T

G
|
G

L
x
A

V

V

G

G

R

A

T

Q

M

M

L

I

Q

K

V

M

L

L

D

E

E

E

R

S

D

T

F

L

P

P

V

I

R

D

E

K

I

I

L

R

P

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

R

A

G

G

R

K

E

S

I

L

V

K

F

F

R

K

G

D

K

Q

S

D

F

I

R

T

T

I

D

E

V

E

P
x
T

S

T

A

E

E

T

V

A

F

F

S

E

R

G

A

V

D

D

I

I

A

A

L

L

F

F

S

S

A
|
A

G

G

A

S

T

S

A
x
T

S

S

R

A

T

K

W

Y

A

A

S

P

V

Y

A

A

A

V

E

K

A

A

G

G

A

V

V

V

I

V

D

D

N

N

S

T

S

S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

A

A

I

L

F

-

D

-

V

-

Q

-

G

D

K

A

P

H

K

N

G

G

V

I

I

I

A

A

N

C

P

P

N
|
N

C

C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

R

K

Y

W

R

G

V

L

R

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q

Q

S

A

V

V

T

S

G
|
G

K
x
A

G
|
G

-

M

-

G

-

A

-

I

-

L

-

E

-

T

-

Q

K

R

Q

E

G

L

R

R

D

E

A

V

L

L

E

N

D

D

E

G

L

V

-

K

-

P

-

C

-

D

-

L

-

H

A

A

G

E

K

I

M

L

P

P

E

S

S

G

F

G

V

D

H

K

G

K

H

H

Q

Y

-

P

I

I

A

A

F

F

N

N

A

A

V

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

D

D

N

N

G

D

Y

Y

T

T

K

Y

E
|
E

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

R

K

K

K

I

I

M

M

C

E

D

D

E

D

S

S

L

I

S

A

V

V

S

S

P

A

T

T

T

C

V

V

R
|
R

I

I

P

P

V

V

Y

L

G

S

G

A

H

H

G

S

E

E

S

S

M

V

N

Y

I

I

E

E

F

T

E

K

D

E

D

V

F

A

D

P

V

I

D

E

E

E

L

V

R

K

E

A

M

A

L

I

A

A

G

A

T

F

P

P

G

G

I

A

I

V

V

L

Q

E

D

D

P

V

S

A

S

H

G

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

T

N

S

A

Y

V

E

G

K

S

D

R

E

D

V

T

F

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

F

L

W

D

Q

A

P

E

K

K

T

G

V

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

L

L

R

L

K

K

G

G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

K

T

L

L

binding 5'-o-monophosphoryladenylyl(2'->5')adenylyl(2'->5')adenosine: G8 (= G24), T10 (= T26), G11 (= G27), A12 (≠ L28), A35 (= A51), S36 (= S52), R38 (= R54), S39 (= S55), T56 (≠ P72), A71 (= A87), T75 (≠ A91), G160 (= G180)
binding trans-cyclohexane-1,4-dicarboxylic acid: R98 (= R114), N126 (= N146), G158 (= G178), A159 (≠ K179), E219 (= E224), K222 (= K227), R244 (= R249)

2gz3A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with NADP and aspartate- semialdehyde (see paper)
45% identity, 94% coverage: 18:345/349 of query aligns to 2:340/357 of 2gz3A

query
sites
2gz3A

Y

Y

S

T

I

V

A

A

I

V

L

V

G
|
G

A

A

T
|
T

G
|
G

L
x
A

V
|
V

G

G

R

A

T

Q

M

M

L

I

Q

K

V

M

L

L

D

E

E

E

R

S

D

T

F

L

P

P

V

I

R

D

E

K

I

I

L

R

P

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

R

A

G

G

R

K

E

S

I

L

V

K

F

F

R

K

G

D

K

Q

S

D

F

I

R

T

T

I

D

E

V

E

P
x
T

S

T

A

E

E

T

V

A

F

F

S

E

R

G

A

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

A

S

T

S

A
x
T

S

S

R

A

T

K

W

Y

A

A

S

P

V

Y

A

A

A

V

E

K

A

A

G

G

A

V

V

V

I

V

D

D

N
|
N

S

T

S

S

A

Y

F

F

R

R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

A

A

I

L

F

-

D

-

V

-

Q

-

G

D

K

A

P

H

K

N

G

G

V

I

I

I

A

A

N

C

P

P

N

N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

R

K

Y

W

R

G

V

L

R

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

S

A

V

V

T

S

G
|
G

K

A

G
|
G

-
x
M

-

G

-

A

-

I

-

L

-

E

-

T

-

Q

K

R

Q

E

G

L

R

R

D

E

A

V

L

L

E

N

D

D

E

G

L

V

-

K

-

P

-

C

-

D

-

L

-

H

A

A

G

E

K

I

M

L

P

P

E

S

S

G

F

G

V

D

H

K

G

K

H

H

Q

Y

-

P

I

I

A

A

F

F

N

N

A

A

V

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

D

D

N

N

G

D

Y

Y

T

T

K

Y

E
|
E

E

E

M

M

K

K

M

M

V

T

N

K

E

E

T

T

R

K

K

K

I

I

M

M

C

E

D

D

E

D

S

S

L

I

S

A

V

V

S

S

P

A

T

T

T

C

V

V

R
|
R

I

I

P

P

V

V

Y

L

G

S

G

A

H
|
H

G

S

E

E

S

S

M

V

N

Y

I

I

E

E

F

T

E

K

D

E

D

V

F

A

D

P

V

I

D

E

E

E

L

V

R

K

E

A

M

A

L

I

A

A

G

A

T

F

P

P

G

G

I

A

I

V

V

L

Q

E

D

D

P

V

S

A

S

H

G

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

T

N

S

A

Y

V

E

G

K

S

D

R

E

D

V

T

F

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

F

L

W

D

Q

A

P

E

K

K

T

G

V

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N
|
N

L

L

R

L

K

K

G

G

A
|
A

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

K

T

L

L

active site: C127 (= C147), Q154 (= Q174), R244 (= R249), H251 (= H256)
binding (2r)-2-amino-4-oxobutanoic acid: C127 (= C147), Q154 (= Q174), G158 (= G178), E219 (= E224), R244 (= R249), H251 (= H256)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G24), T10 (= T26), G11 (= G27), A12 (≠ L28), V13 (= V29), A35 (= A51), S36 (= S52), R38 (= R54), S39 (= S55), T56 (≠ P72), S70 (= S86), A71 (= A87), G72 (= G88), T75 (≠ A91), N93 (= N109), G158 (= G178), G160 (= G180), M161 (vs. gap), N324 (= N329), A329 (= A334)

2gz2A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with 2',5'-adp (see paper)
45% identity, 94% coverage: 18:345/349 of query aligns to 2:340/357 of 2gz2A

query
sites
2gz2A

Y

Y

S

T

I

V

A

A

I

V

L

V

G
|
G

A

A

T
|
T

G
|
G

L

A

V

V

G

G

R

A

T

Q

M

M

L

I

Q

K

V

M

L

L

D

E

E

E

R

S

D

T

F

L

P

P

V

I

R

D

E

K

I

I

L

R

P

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

R

A

G

G

R

K

E

S

I

L

V

K

F

F

R

K

G

D

K

Q

S

D

F

I

R

T

T

I

D

E

V

E

P
x
T

S

T

A

E

E

T

V

A

F

F

S

E

R

G

A

V

D

D

I

I

A

A

L

L

F

F

S

S

A
|
A

G

G

A

S

T

S

A
x
T

S

S

R

A

T

K

W

Y

A

A

S

P

V

Y

A

A

A

V

E

K

A

A

G

G

A

V

V

V

I

V

D

D

N

N

S

T

S

S

A

Y

F

F

R

R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

A

A

I

L

F

-

D

-

V

-

Q

-

G

D

K

A

P

H

K

N

G

G

V

I

I

I

A

A

N

C

P

P

N

N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

R

K

Y

W

R

G

V

L

R

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

S

A

V

V

T

S

G

G

K

A

G

G

-

M

-

G

-

A

-

I

-

L

-

E

-

T

-

Q

K

R

Q

E

G

L

R

R

D

E

A

V

L

L

E

N

D

D

E

G

L

V

-

K

-

P

-

C

-

D

-

L

-

H

A

A

G

E

K

I

M

L

P

P

E

S

S

G

F

G

V

D

H

K

G

K

H

H

Q

Y

-

P

I

I

A

A

F

F

N

N

A

A

V

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

D

D

N

N

G

D

Y

Y

T

T

K

Y

E

E

M

M

K

K

M

M

V

T

N

K

E

E

T

T

R

K

K

K

I

I

M

M

C

E

D

D

E

D

S

S

L

I

S

A

V

V

S

S

P

A

T

T

T

C

V

V

R
|
R

I

I

P

P

V

V

Y

L

G

S

G

A

H
|
H

G

S

E

E

S

S

M

V

N

Y

I

I

E

E

F

T

E

K

D

E

D

V

F

A

D

P

V

I

D

E

E

E

L

V

R

K

E

A

M

A

L

I

A

A

G

A

T

F

P

P

G

G

I

A

I

V

V

L

Q

E

D

D

P

V

S

A

S

H

G

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

T

N

S

A

Y

V

E

G

K

S

D

R

E

D

V

T

F

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

F

L

W

D

Q

A

P

E

K

K

T

G

V

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

L

L

R

L

K

K

G

G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

K

T

L

L

active site: C127 (= C147), Q154 (= Q174), R244 (= R249), H251 (= H256)
binding adenosine-2'-5'-diphosphate: G8 (= G24), T10 (= T26), G11 (= G27), A35 (= A51), S36 (= S52), R38 (= R54), S39 (= S55), T56 (≠ P72), A71 (= A87), T75 (≠ A91)

2gz1A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with NADP (see paper)
45% identity, 94% coverage: 18:345/349 of query aligns to 2:340/357 of 2gz1A

query
sites
2gz1A

Y

Y

S

T

I

V

A

A

I

V

L

V

G
|
G

A

A

T
|
T

G
|
G

L
x
A

V
|
V

G

G

R

A

T

Q

M

M

L

I

Q

K

V

M

L

L

D

E

E

E

R

S

D

T

F

L

P

P

V

I

R

D

E

K

I

I

L

R

P

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

R

A

G

G

R

K

E

S

I

L

V

K

F

F

R

K

G

D

K

Q

S

D

F

I

R

T

T

I

D

E

V

E

P
x
T

S

T

A

E

E

T

V

A

F

F

S

E

R

G

A

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

A

S

T

S

A
x
T

S

S

R

A

T

K

W

Y

A

A

S

P

V

Y

A

A

A

V

E

K

A

A

G

G

A

V

V

V

I

V

D

D

N
|
N

S

T

S

S

A

Y

F

F

R

R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

A

A

I

L

F

-

D

-

V

-

Q

-

G

D

K

A

P

H

K

N

G

G

V

I

I

I

A

A

N

C

P

P

N

N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

R

K

Y

W

R

G

V

L

R

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

S

A

V

V

T
x
S

G
|
G

K

A

G
|
G

-
x
M

-

G

-

A

-

I

-

L

-

E

-

T

-

Q

K

R

Q

E

G

L

R

R

D

E

A

V

L

L

E

N

D

D

E

G

L

V

-

K

-

P

-

C

-

D

-

L

-

H

A

A

G

E

K

I

M

L

P

P

E

S

S

G

F

G

V

D

H

K

G

K

H

H

Q

Y

-

P

I

I

A

A

F

F

N

N

A

A

V

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

D

D

N

N

G

D

Y

Y

T

T

K

Y

E

E

M

M

K

K

M

M

V

T

N

K

E

E

T

T

R

K

K

K

I

I

M

M

C

E

D

D

E

D

S

S

L

I

S

A

V

V

S

S

P

A

T

T

T

C

V

V

R
|
R

I

I

P

P

V

V

Y

L

G

S

G

A

H
|
H

G

S

E

E

S

S

M

V

N

Y

I

I

E

E

F

T

E

K

D

E

D

V

F

A

D

P

V

I

D

E

E

E

L

V

R

K

E

A

M

A

L

I

A

A

G

A

T

F

P

P

G

G

I

A

I

V

V

L

Q

E

D

D

P

V

S

A

S

H

G

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

T

N

S

A

Y

V

E

G

K

S

D

R

E

D

V

T

F

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

F

L

W

D

Q

A

P

E

K

K

T

G

V

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N
|
N

L
|
L

R

L

K

K

G

G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

K

T

L

L

active site: C127 (= C147), Q154 (= Q174), R244 (= R249), H251 (= H256)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G24), T10 (= T26), G11 (= G27), A12 (≠ L28), V13 (= V29), A35 (= A51), S36 (= S52), R38 (= R54), S39 (= S55), T56 (≠ P72), S70 (= S86), A71 (= A87), G72 (= G88), T75 (≠ A91), N93 (= N109), S157 (≠ T177), G158 (= G178), G160 (= G180), M161 (vs. gap), N324 (= N329), L325 (= L330)

3q11A Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with NADP and aspartyl beta- difluorophosphonate (see paper)
45% identity, 94% coverage: 18:345/349 of query aligns to 2:340/358 of 3q11A

query
sites
3q11A

Y

Y

S

T

I

V

A

A

I

V

L

V

G
|
G

A

A

T
|
T

G
|
G

L

A

V

V

G

G

R

A

T

Q

M

M

L

I

Q

K

V

M

L

L

D

E

E

E

R

S

D

T

F

L

P

P

V

I

R

D

E

K

I

I

L

R

P

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

R

A

G

G

R

K

E

S

I

L

V

K

F

F

R

K

G

D

K

Q

S

D

F

I

R

T

T

I

D

E

V

E

P
x
T

S

T

A

E

E

T

V

A

F

F

S

E

R

G

A

V

D

D

I

I

A

A

L

L

F

F

S

S

A
|
A

G

G

A

S

T

S

A
x
T

S

S

R

A

T

K

W

Y

A

A

S

P

V

Y

A

A

A

V

E

K

A

A

G

G

A

V

V

V

I

V

D

D

N

N

S

T

S

S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

A

A

I

L

F

-

D

-

V

-

Q

-

G

D

K

A

P

H

K

N

G

G

V

I

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

R

K

Y

W

R

G

V

L

R

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

S

A

V

V

T

S

G
|
G

K

A

G
|
G

-
x
M

-
x
G

-

A

-

I

-

L

-

E

-

T

-

Q

K

R

Q

E

G

L

R

R

D

E

A

V

L

L

E

N

D

D

E

G

L

V

-

K

-

P

-

C

-

D

-

L

-

H

A

A

G

E

K

I

M

L

P

P

E

S

S

G

F

G

V

D

H

K

G

K

H

H

Q

Y

-

P

I

I

A

A

F

F

N

N

A

A

V

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

D

D

N

N

G

D

Y

Y

T

T

K

Y

E
|
E

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

R

K

K

K

I

I

M

M

C

E

D

D

E

D

S

S

L

I

S

A

V

V

S

S

P

A

T

T

T

C

V

V

R
|
R

I

I

P

P

V

V

Y

L

G

S

G

A

H

H

G

S

E

E

S

S

M

V

N

Y

I

I

E

E

F

T

E

K

D

E

D

V

F

A

D

P

V

I

D

E

E

E

L

V

R

K

E

A

M

A

L

I

A

A

G

A

T

F

P

P

G

G

I

A

I

V

V

L

Q

E

D

D

P

V

S

A

S

H

G

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

T

N

S

A

Y

V

E

G

K

S

D

R

E

D

V

T

F

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

F

L

W

D

Q

A

P

E

K

K

T

G

V

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

L

L

R

L

K

K

G

G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

K

T

L

L

binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G24), T10 (= T26), G11 (= G27), A35 (= A51), S36 (= S52), R38 (= R54), S39 (= S55), T56 (≠ P72), A71 (= A87), T75 (≠ A91), G160 (= G180), M161 (vs. gap), G162 (vs. gap)
binding 5,5-difluoro-4-oxo-5-phosphono-D-norvaline: R98 (= R114), N126 (= N146), C127 (= C147), Q154 (= Q174), G158 (= G178), E219 (= E224), K222 (= K227), R244 (= R249)

4r5hA Crystal structure of sp-aspartate-semialdehyde-dehydrogenase with nicotinamide-adenine-dinucleotide-phosphate and 3-carboxy-propenyl- phthalic acid (see paper)
45% identity, 94% coverage: 18:345/349 of query aligns to 2:340/359 of 4r5hA

query
sites
4r5hA

Y

Y

S

T

I

V

A

A

I

V

L

V

G
|
G

A

A

T
|
T

G
|
G

L
x
A

V
|
V

G

G

R

A

T

Q

M

M

L

I

Q

K

V

M

L

L

D

E

E

E

R

S

D

T

F

L

P

P

V

I

R

D

E

K

I

I

L

R

P

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

R

A

G

G

R

K

E

S

I

L

V

K

F

F

R

K

G

D

K

Q

S

D

F

I

R

T

T

I

D

E

V

E

P
x
T

S

T

A

E

E

T

V

A

F

F

S

E

R

G

A

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

A
x
S

T

S

A
x
T

S

S

R

A

T

K

W

Y

A

A

S

P

V

Y

A

A

A

V

E

K

A

A

G

G

A

V

V

V

I

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

A

A

I

L

F

-

D

-

V

-

Q

-

G

D

K

A

P

H

K

N

G

G

V

I

I

I

A

A

N

C

P
|
P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

R

K

Y

W

R

G

V

L

R

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

S

A

V

V

T

S

G
|
G

K

A

G
|
G

-
x
M

-

G

-

A

-

I

-

L

-

E

-

T

-

Q

K

R

Q

E

G

L

R

R

D

E

A

V

L

L

E

N

D

D

E

G

L

V

-

K

-

P

-

C

-

D

-

L

-

H

A

A

G

E

K

I

M

L

P

P

E

S

S

G

F

G

V

D

H

K

G

K

H

H

Q

Y

-

P

I

I

A

A

F

F

N

N

A

A

V

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

D

D

N

N

G

D

Y

Y

T

T

K

Y

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

R

K

K

K

I

I

M

M

C

E

D

D

E

D

S

S

L

I

S

A

V

V

S

S

P

A

T

T

T

C

V

V

R
|
R

I

I

P

P

V

V

Y

L

G

S

G

A

H
|
H

G

S

E

E

S

S

M

V

N

Y

I

I

E

E

F

T

E

K

D

E

D

V

F

A

D

P

V

I

D

E

E

E

L

V

R

K

E

A

M

A

L

I

A

A

G

A

T

F

P

P

G

G

I

A

I

V

V

L

Q

E

D

D

P

V

S

A

S

H

G

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

T

N

S

A

Y

V

E

G

K

S

D

R

E

D

V

T

F

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

F

L

W

D

Q

A

P

E

K

K

T

G

V

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

L

L

R

L

K

K

G
|
G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

K

T

L

L

binding 3-[(1E)-3-carboxyprop-1-en-1-yl]benzene-1,2-dicarboxylic acid: S73 (≠ A89), T94 (≠ S110), S95 (= S111), R98 (= R114), N126 (= N146), C127 (= C147), Q154 (= Q174), G158 (= G178), K222 (= K227), R244 (= R249), H251 (= H256)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G24), T10 (= T26), G11 (= G27), A12 (≠ L28), V13 (= V29), A35 (= A51), S36 (= S52), R38 (= R54), S39 (= S55), T56 (≠ P72), S70 (= S86), A71 (= A87), G72 (= G88), T75 (≠ A91), N93 (= N109), T94 (≠ S110), P125 (= P145), N126 (= N146), C127 (= C147), G160 (= G180), M161 (vs. gap), G328 (= G333)

4r4jA Crystal structure of complex sp_asadh with 3-carboxypropyl-phthalic acid and nicotinamide adenine dinucleotide phosphate (see paper)
45% identity, 94% coverage: 18:345/349 of query aligns to 2:340/359 of 4r4jA

query
sites
4r4jA

Y

Y

S

T

I

V

A

A

I

V

L

V

G
|
G

A

A

T
|
T

G
|
G

L
x
A

V
|
V

G

G

R

A

T

Q

M

M

L

I

Q

K

V

M

L

L

D

E

E

E

R

S

D

T

F

L

P

P

V

I

R

D

E

K

I

I

L

R

P

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

R

A

G

G

R

K

E

S

I

L

V

K

F

F

R

K

G

D

K

Q

S

D

F

I

R

T

T

I

D

E

V

E

P
x
T

S

T

A

E

E

T

V

A

F

F

S

E

R

G

A

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

A

S

T

S

A
x
T

S

S

R

A

T

K

W

Y

A

A

S

P

V

Y

A

A

A

V

E

K

A

A

G

G

A

V

V

V

I

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

A

A

I

L

F

-

D

-

V

-

Q

-

G

D

K

A

P

H

K

N

G

G

V

I

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

R

K

Y

W

R

G

V

L

R

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

S

A

V

V

T

S

G
|
G

K

A

G
|
G

-
x
M

-

G

-

A

-

I

-

L

-

E

-

T

-

Q

K

R

Q

E

G

L

R

R

D

E

A

V

L

L

E

N

D

D

E

G

L

V

-

K

-

P

-

C

-

D

-

L

-

H

A

A

G

E

K

I

M

L

P

P

E

S

S

G

F

G

V

D

H

K

G

K

H

H

Q

Y

-

P

I

I

A

A

F

F

N

N

A

A

V

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

D

D

N

N

G

D

Y

Y

T

T

K

Y

E
|
E

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

R

K

K

K

I

I

M

M

C

E

D

D

E

D

S

S

L

I

S

A

V

V

S

S

P

A

T

T

T

C

V

V

R
|
R

I

I

P

P

V

V

Y

L

G

S

G

A

H
|
H

G

S

E

E

S

S

M

V

N

Y

I

I

E

E

F

T

E

K

D

E

D

V

F

A

D

P

V

I

D

E

E

E

L

V

R

K

E

A

M

A

L

I

A

A

G

A

T

F

P

P

G

G

I

A

I

V

V

L

Q

E

D

D

P

V

S

A

S

H

G

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

T

N

S

A

Y

V

E

G

K

S

D

R

E

D

V

T

F

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

F

L

W

D

Q

A

P

E

K

K

T

G

V

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

L

L

R

L

K

K

G
|
G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

K

T

L

L

binding 3-(3-carboxypropyl)benzene-1,2-dicarboxylic acid: T94 (≠ S110), S95 (= S111), R98 (= R114), N126 (= N146), C127 (= C147), Q154 (= Q174), G158 (= G178), E219 (= E224), K222 (= K227), R244 (= R249), H251 (= H256)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G24), T10 (= T26), G11 (= G27), A12 (≠ L28), V13 (= V29), A35 (= A51), S36 (= S52), R38 (= R54), S39 (= S55), T56 (≠ P72), S70 (= S86), A71 (= A87), G72 (= G88), T75 (≠ A91), N93 (= N109), T94 (≠ S110), N126 (= N146), C127 (= C147), G160 (= G180), M161 (vs. gap), G328 (= G333)

3pyxB Crystals structure of aspartate beta-semialdehyde dehydrogenase complex with NADP and 2-aminoterephthalate (see paper)
45% identity, 94% coverage: 18:345/349 of query aligns to 2:340/359 of 3pyxB

query
sites
3pyxB

Y

Y

S

T

I

V

A

A

I

V

L

V

G
|
G

A

A

T
|
T

G
|
G

L
x
A

V
|
V

G

G

R

A

T

Q

M

M

L

I

Q

K

V

M

L

L

D

E

E

E

R

S

D

T

F

L

P

P

V

I

R

D

E

K

I

I

L

R

P

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

R

A

G

G

R

K

E

S

I

L

V

K

F

F

R

K

G

D

K

Q

S

D

F

I

R

T

T

I

D

E

V

E

P
x
T

S

T

A

E

E

T

V

A

F

F

S

E

R

G

A

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

A

S

T

S

A
x
T

S

S

R

A

T

K

W

Y

A

A

S

P

V

Y

A

A

A

V

E

K

A

A

G

G

A

V

V

V

I

V

D

D

N

N

S

T

S

S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

A

A

I

L

F

-

D

-

V

-

Q

-

G

D

K

A

P

H

K

N

G

G

V

I

I

I

A

A

N

C

P

P

N

N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

R

K

Y

W

R

G

V

L

R

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q

Q

S

A

V

V

T
x
S

G
|
G

K

A

G
|
G

-
x
M

-

G

-

A

-

I

-

L

-

E

-

T

-

Q

K

R

Q

E

G

L

R

R

D

E

A

V

L

L

E

N

D

D

E

G

L

V

-

K

-

P

-

C

-

D

-

L

-

H

A

A

G

E

K

I

M

L

P

P

E

S

S

G

F

G

V

D

H

K

G

K

H

H

Q

Y

-

P

I

I

A

A

F

F

N

N

A

A

V

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

D

D

N

N

G

D

Y

Y

T

T

K

Y

E
|
E

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

R

K

K

K

I

I

M

M

C

E

D

D

E

D

S

S

L

I

S

A

V

V

S

S

P

A

T

T

T

C

V

V

R
|
R

I

I

P

P

V

V

Y

L

G

S

G

A

H

H

G

S

E

E

S

S

M

V

N

Y

I

I

E

E

F

T

E

K

D

E

D

V

F

A

D

P

V

I

D

E

E

E

L

V

R

K

E

A

M

A

L

I

A

A

G

A

T

F

P

P

G

G

I

A

I

V

V

L

Q

E

D

D

P

V

S

A

S

H

G

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

T

N

S

A

Y

V

E

G

K

S

D

R

E

D

V

T

F

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

F

L

W

D

Q

A

P

E

K

K

T

G

V

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N
|
N

L
|
L

R

L

K

K

G

G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

K

T

L

L

binding 2-aminobenzene-1,4-dicarboxylic acid: R98 (= R114), G158 (= G178), E219 (= E224), K222 (= K227), R244 (= R249)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G24), T10 (= T26), G11 (= G27), A12 (≠ L28), V13 (= V29), A35 (= A51), S36 (= S52), R38 (= R54), S39 (= S55), T56 (≠ P72), S70 (= S86), A71 (= A87), G72 (= G88), T75 (≠ A91), C127 (= C147), S157 (≠ T177), G158 (= G178), G160 (= G180), M161 (vs. gap), N324 (= N329), L325 (= L330)

3pylC Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with d-2,3-diaminopropionate (see paper)
45% identity, 94% coverage: 18:345/349 of query aligns to 2:340/361 of 3pylC

query
sites
3pylC

Y

Y

S

T

I

V

A

A

I

V

L

V

G

G

A

A

T

T

G

G

L

A

V

V

G

G

R

A

T

Q

M

M

L

I

Q

K

V

M

L

L

D

E

E

E

R

S

D

T

F

L

P

P

V

I

R

D

E

K

I

I

L

R

P

Y

L

L

A

A

S

S

E

A

R

R

S

S

R

A

G

G

R

K

E

S

I

L

V

K

F

F

R

K

G

D

K

Q

S

D

F

I

R

T

T

I

D

E

V

E

P

T

S

T

A

E

E

T

V

A

F

F

S

E

R

G

A

V

D

D

I

I

A

A

L

L

F

F

S

S

A

A

G

G

A

S

T

S

A

T

S

S

R

A

T

K

W

Y

A

A

S

P

V

Y

A

A

A

V

E

K

A

A

G

G

A

V

V

V

I

V

D

D

N

N

S

T

S

S

A

Y

F

F

R

R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

A

A

I

L

F

-

D

-

V

-

Q

-

G

D

K

A

P

H

K

N

G

G

V

I

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

R

K

Y

W

R

G

V

L

R

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q

Q

S

A

V

V

T

S

G
|
G

K

A

G

G

-

M

-

G

-

A

-

I

-

L

-

E

-

T

-

Q

K

R

Q

E

G

L

R

R

D

E

A

V

L

L

E

N

D

D

E

G

L

V

-

K

-

P

-

C

-

D

-

L

-

H

A

A

G

E

K

I

M

L

P

P

E

S

S

G

F

G

V

D

H

K

G

K

H

H

Q

Y

-

P

I

I

A

A

F

F

N

N

A

A

V

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

D

D

N

N

G

D

Y

Y

T

T

K

Y

E
|
E

E

E

M

M

K

K

M

M

V

T

N

K

E

E

T

T

R

K

K

K

I

I

M

M

C

E

D

D

E

D

S

S

L

I

S

A

V

V

S

S

P

A

T

T

T

C

V

V

R
|
R

I

I

P

P

V

V

Y

L

G

S

G

A

H

H

G

S

E

E

S

S

M

V

N

Y

I

I

E

E

F

T

E

K

D

E

D

V

F

A

D

P

V

I

D

E

E

E

L

V

R

K

E

A

M

A

L

I

A

A

G

A

T

F

P

P

G

G

I

A

I

V

V

L

Q

E

D

D

P

V

S

A

S

H

G

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

T

N

S

A

Y

V

E

G

K

S

D

R

E

D

V

T

F

F

V

V

G

G

R

R

I

I

R

R

R

K

D

D

F

L

W

D

Q

A

P

E

K

K

T

G

V

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

L

L

R

L

K

K

G

G

A

A

T

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

K

T

L

L

binding 3-amino-D-alanine: N126 (= N146), C127 (= C147), G158 (= G178), E219 (= E224), R244 (= R249)

4r51A Crystal complex structure of sp-aspartate-semialdehyde-dehydrogenase with nicotinamide adenine dinucleotide phosphate and phthalic acid (see paper)
45% identity, 94% coverage: 18:345/349 of query aligns to 2:340/360 of 4r51A

query
sites
4r51A

Y

Y

S

T

I

V

A

A

I

V

L

V

G
|
G

A

A

T
|
T

G
|
G

L
x
A

V
|
V

G

G

R

A

T

Q

M

M

L

I

Q

K

V

M

L

L

D

E

E

E

R

S

D

T

F

L

P

P

V

I

R

D

E

K

I

I

L

R

P

Y

L

L

A
|
A

S
|
S

E

A

R

R

S
|
S

R

A

G

G

R

K

E

S

I

L

V

K

F

F

R

K

G

D

K

Q

S

D

F

I

R

T

T

I

D

E

V

E

P
x
T

S

T

A

E

E

T

V

A

F

F

S

E

R

G

A

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

A
x
S

T

S

A

T

S

S

R

A

T

K

W

Y

A

A

S

P

V

Y

A

A

A

V

E

K

A

A

G

G

A

V

V

V

I

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

F

F

R
|
R

M

Q

E

N

P

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

A

A

I

L

F

-

D

-

V

-

Q

-

G

D

K

A

P

H

K

N

G

G

V

I

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

L

V

H

R

D

Q

R

K

Y

W

R

G

V

L

R

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q

Q

S

A

V

V

T

S

G

G

K

A

G
|
G

-
x
M

-

G

-

A

-

I

-

L

-

E

-

T

-

Q

K

R

Q

E

G

L

R

R

D

E

A

V

L

L

E

N

D

D

E

G

L

V

-

K

-

P

-

C

-

D

-

L

-

H

A

A

G

E

K

I

M

L

P

P

E

S

S

G

F

G

V

D

H

K

G

K

H

H

Q

Y

-

P

I

I

A

A

F

F

N

N

A

A

V

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

D

D

N

N

G

D

Y

Y

T

T

K

Y

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

R

K

K

K

I

I

M

M

C

E

D

D

E

D

S

S

L

I

S

A

V

V

S

S

P

A

T

T

T

C

V

V

R

R

I

I

P

P

V

V

Y

L

G

S

G

A

H

H

G

S

E

E

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S

M

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Y

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D

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A

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N

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D

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N

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G

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I

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A

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E

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L

binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G24), T10 (= T26), G11 (= G27), A12 (≠ L28), V13 (= V29), A35 (= A51), S36 (= S52), S39 (= S55), T56 (≠ P72), S70 (= S86), A71 (= A87), G72 (= G88), N93 (= N109), T94 (≠ S110), N126 (= N146), C127 (= C147), G160 (= G180), M161 (vs. gap), G328 (= G333)
binding phthalic acid: S73 (≠ A89), T94 (≠ S110), S95 (= S111), R98 (= R114), N126 (= N146), K222 (= K227)

8jusA Crystal structure of aspartate semialdehyde dehydrogenase from porphyromonas gingivalis complexed with 2',5'adenosine diphosphate
46% identity, 94% coverage: 20:348/349 of query aligns to 3:332/335 of 8jusA

query
sites
8jusA

I

I

A

A

I

I

L

V

G
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G

A

V

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x
S

G
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A

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G

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F

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K

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Y

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-

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A

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T

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I

A

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N

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L

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E

E

E

L

L

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A

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R

G

G

K

E

M

K

P

P

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F

D

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K

H

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A

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Y

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N

N

A

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P

H

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I

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D

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F

F

L

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D

D

N

N

G

D

Y

Y

T

T

K

K

E

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M

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K

M

M

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Y

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R

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I

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M

C

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A

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N

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binding adenosine-2'-5'-diphosphate: G7 (= G24), S9 (≠ T26), G10 (= G27), G34 (≠ A51), S35 (= S52), R37 (= R54), S38 (= S55), S70 (= S86), A71 (= A87), T75 (≠ A91)

3tz6A Crystal structure of aspartate semialdehyde dehydrogenase complexed with inhibitor smcs (cys) and phosphate from mycobacterium tuberculosis h37rv (see paper)
44% identity, 94% coverage: 18:345/349 of query aligns to 2:340/342 of 3tz6A

query
sites
3tz6A

Y

L

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S

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A

A

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A

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A

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I

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A

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R

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S

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I

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x
H

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x
R

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K

K

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A

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M

A

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M

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G

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I

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A

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F

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A

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D

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H

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N

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E

E

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x
E

E

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R

E

E

M

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L

L

A

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G

G

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A

P

T

G

G

I

V

I

Q

V

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Q

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D

D

-

P

-

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-

G

-

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-

Y

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M

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L

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A

Y

A

E

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K

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E

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G

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R

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I

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R

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D

F

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P

-

D

-

G

K

R

T

G

V

L

N

A

L

L

W

F

I

V

V

S

A

G

D

D

N

N

L

L

R

R

K

K

G

G

A

A

T

L

N

N

A

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|
Q

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I

A

A

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|
E

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x
L

L

L

active site: C129 (= C147), Q156 (= Q174), R248 (= R249), H255 (= H256)
binding cysteine: C129 (= C147), Q156 (= Q174), G160 (= G178), E223 (= E224), R248 (= R249), H255 (= H256)
binding glycerol: S108 (≠ P124), G187 (vs. gap), F192 (≠ M195), P201 (≠ Q204), Q225 (≠ M226), R228 (≠ V229), F229 (≠ N230), Q335 (= Q340), E338 (= E343), L339 (≠ K344)
binding sulfate ion: R98 (= R114), H117 (≠ Q135), R119 (≠ K137), N128 (= N146), C129 (= C147), K226 (= K227), E270 (≠ D271), R273 (= R274)

Query Sequence

>642684670 IMG__ChlphaBS1_FD:642684670
MTTNKEAENIMSSTEKTYSIAILGATGLVGRTMLQVLDERDFPVREILPLASERSRGREI
VFRGKSFRTDVPSAEVFSRADIALFSAGATASRTWASVAAEAGAVVIDNSSAFRMEPDVP
LVVPEVNPEAIFDVQGKPKGVIANPNCSTIQMVVVLKPLHDRYRVRRVVVSTYQSVTGKG
KQGRDALEDELAGKMPESFVHGHQIAFNAVPHIDVFLDNGYTKEEMKMVNETRKIMCDES
LSVSPTTVRIPVYGGHGESMNIEFEDDFDVDELREMLAGTPGIIVQDDPSSGLYPMPLTS
YEKDEVFVGRIRRDFWQPKTVNLWIVADNLRKGAATNAVQIAEKLVGKL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory