SitesBLAST
Comparing 642684930 IMG__ChlphaBS1_FD:642684930 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6ed7A Crystal structure of 7,8-diaminopelargonic acid synthase bound to inhibitor mac13772
62% identity, 97% coverage: 4:424/434 of query aligns to 2:421/429 of 6ed7A
- active site: Y17 (= Y19), Y144 (= Y147), D245 (= D248), K274 (= K277)
- binding 2-[(2-nitrophenyl)sulfanyl]acetohydrazide: Y17 (= Y19), W52 (= W54), W52 (= W54), Y144 (= Y147), D147 (= D150), A217 (= A220), K274 (= K277), R391 (= R394), F393 (= F396), F393 (= F396)
- binding pyridoxal-5'-phosphate: G112 (= G114), S113 (= S115), Y144 (= Y147), H145 (= H148), D245 (= D248), I247 (= I250), K274 (= K277)
P12995 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase; 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; Diaminopelargonic acid synthase; EC 2.6.1.62 from Escherichia coli (strain K12) (see 4 papers)
62% identity, 96% coverage: 4:420/434 of query aligns to 2:417/429 of P12995
- Y17 (= Y19) mutation to F: Severely reduces the aminotransferase activity.
- W52 (= W54) binding substrate
- GS 112:113 (= GS 114:115) binding pyridoxal 5'-phosphate
- Y144 (= Y147) mutation to F: Severely reduces the aminotransferase activity.
- D147 (= D150) mutation to N: Loss of aminotransferase activity.
- D245 (= D248) binding pyridoxal 5'-phosphate
- R253 (= R256) mutation to A: Has only a small effect on the rate of reaction with DAPA.; mutation to K: Increases aminotransferase activity toward SAM.; mutation to M: Loss of aminotransferase activity.; mutation to Q: Increases aminotransferase activity toward SAM.
- K274 (= K277) binding substrate; modified: N6-(pyridoxal phosphate)lysine
- G307 (= G310) binding substrate
- PT 308:309 (= PT 311:312) binding pyridoxal 5'-phosphate
- R391 (= R394) binding substrate; mutation to A: Reduces aminotransferase activity.
1dtyA Crystal structure of adenosylmethionine-8-amino-7-oxonanoate aminotransferase with pyridoxal phosphate cofactor.
62% identity, 96% coverage: 4:420/434 of query aligns to 2:417/429 of 1dtyA
- active site: Y17 (= Y19), Y144 (= Y147), E211 (= E214), D245 (= D248), A248 (= A251), K274 (= K277), Y398 (= Y401)
- binding pyridoxal-5'-phosphate: G112 (= G114), S113 (= S115), Y144 (= Y147), H145 (= H148), D245 (= D248), I247 (= I250), K274 (= K277)
1mlzA Crystal structure of 7,8-diaminopelargonic acid synthase in complex with the trans-isomer of amiclenomycin. (see paper)
62% identity, 96% coverage: 4:420/434 of query aligns to 2:416/427 of 1mlzA
- active site: Y17 (= Y19), Y144 (= Y147), E210 (= E214), D244 (= D248), A247 (= A251), K273 (= K277), Y397 (= Y401)
- binding pyridoxal-5'-phosphate: G112 (= G114), S113 (= S115), Y144 (= Y147), H145 (= H148), D244 (= D248), I246 (= I250), K273 (= K277), P307 (= P311), T308 (= T312)
- binding trans-amiclenomycin: W52 (= W54), W53 (= W55), Y144 (= Y147), K273 (= K277), R390 (= R394), F392 (= F396)
1mlyA Crystal structure of 7,8-diaminopelargonic acid synthase in complex with the cis isomer of amiclenomycin (see paper)
62% identity, 96% coverage: 4:420/434 of query aligns to 2:416/427 of 1mlyA
- active site: Y17 (= Y19), Y144 (= Y147), E210 (= E214), D244 (= D248), A247 (= A251), K273 (= K277), Y397 (= Y401)
- binding cis-amiclenomycin: W52 (= W54), W53 (= W55), K273 (= K277), R390 (= R394), F392 (= F396)
- binding pyridoxal-5'-phosphate: G112 (= G114), S113 (= S115), Y144 (= Y147), H145 (= H148), D244 (= D248), I246 (= I250), K273 (= K277), P307 (= P311), T308 (= T312)
1qj3A Crystal structure of 7,8-diaminopelargonic acid synthase in complex with 7-keto-8-aminopelargonic acid (see paper)
61% identity, 96% coverage: 4:420/434 of query aligns to 2:405/416 of 1qj3A
- active site: Y17 (= Y19), Y144 (= Y147), E201 (= E214), D235 (= D248), A238 (= A251), K264 (= K277), Y386 (= Y401)
- binding 7-keto-8-aminopelargonic acid: Y17 (= Y19), W52 (= W54), Y144 (= Y147), K264 (= K277), R379 (= R394), F381 (= F396)
- binding pyridoxal-5'-phosphate: G112 (= G114), S113 (= S115), Y144 (= Y147), H145 (= H148), G146 (= G149), D235 (= D248), I237 (= I250), A238 (= A251), K264 (= K277)
4cxqA Mycobaterium tuberculosis transaminase bioa complexed with substrate kapa (see paper)
55% identity, 96% coverage: 6:423/434 of query aligns to 5:421/427 of 4cxqA
- active site: Y18 (= Y19), Y149 (= Y147), E212 (= E214), D246 (= D248), A249 (= A251), K275 (= K277), Y399 (= Y401)
- binding 7-keto-8-aminopelargonic acid: W56 (= W54), Y149 (= Y147), G308 (= G310), T310 (= T312), R392 (= R394)
- binding pyridoxal-5'-phosphate: G116 (= G114), S117 (= S115), Y149 (= Y147), H150 (= H148), G151 (= G149), E212 (= E214), D246 (= D248), I248 (= I250), K275 (= K277), P309 (= P311), T310 (= T312)
4w1vA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a thiazole inhibitor (see paper)
55% identity, 96% coverage: 6:423/434 of query aligns to 5:419/425 of 4w1vA
- active site: Y18 (= Y19), Y147 (= Y147), E210 (= E214), D244 (= D248), A247 (= A251), K273 (= K277), Y397 (= Y401)
- binding dimethyl (2R)-5-(3-fluorophenyl)-1H-pyrrolo[1,2-c][1,3]thiazole-6,7-dicarboxylate 2-oxide: P17 (= P18), Y18 (= Y19), W54 (= W54), M81 (= M81), G83 (= G83), Y147 (= Y147), G306 (= G310), P307 (= P311), T308 (= T312), F392 (= F396)
- binding pyridoxal-5'-phosphate: G114 (= G114), S115 (= S115), Y147 (= Y147), H148 (= H148), E210 (= E214), D244 (= D248), I246 (= I250), K273 (= K277)
4cxrA Mycobaterium tuberculosis transaminase bioa complexed with 1-(1,3- benzothiazol-2-yl)methanamine (see paper)
55% identity, 96% coverage: 6:423/434 of query aligns to 5:419/425 of 4cxrA
- active site: Y18 (= Y19), Y147 (= Y147), E210 (= E214), D244 (= D248), A247 (= A251), K273 (= K277), Y397 (= Y401)
- binding 1-(1,3-benzothiazol-2-yl)methanamine: Y18 (= Y19), W54 (= W54), W55 (= W55), A216 (= A220)
- binding pyridoxal-5'-phosphate: G114 (= G114), S115 (= S115), Y147 (= Y147), H148 (= H148), E210 (= E214), D244 (= D248), I246 (= I250), K273 (= K277), P307 (= P311), T308 (= T312)
P9WQ81 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase; 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; 7,8-diaminopelargonic acid synthase; DAPAS; Diaminopelargonic acid synthase; EC 2.6.1.62 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
55% identity, 96% coverage: 6:423/434 of query aligns to 12:429/437 of P9WQ81
- Y25 (= Y19) mutation to A: Does not show detectable activity at 335 nm with SAM, even up to concentrations of 3 mM, and shows approximately 70% reduced activity with high concentrations of DAPA (0.5 mM).
- W64 (= W54) binding S-adenosyl-L-methionine
- Y157 (= Y147) binding S-adenosyl-L-methionine
- K283 (= K277) modified: N6-(pyridoxal phosphate)lysine
- G316 (= G310) binding S-adenosyl-L-methionine
5kgtA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with an inhibitor optimized from hts lead: 1-[4-[4-(3-chlorophenyl)carbonylpiperidin-1- yl]phenyl]ethanone (see paper)
55% identity, 96% coverage: 6:423/434 of query aligns to 5:422/429 of 5kgtA