SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 6935922 FitnessBrowser__SB2B:6935922 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P12282 Molybdopterin-synthase adenylyltransferase; MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase; Sulfur carrier protein MoaD adenylyltransferase; EC 2.7.7.80 from Escherichia coli (strain K12) (see 2 papers)
63% identity, 96% coverage: 6:250/254 of query aligns to 4:248/249 of P12282

query
sites
P12282

L

L

S

S

D

D

T

Q

E

E

L

M

L

L

R

R

Y

Y

S

N

R
|
R

Q

Q

I

I

S

I

I

L

K

R

S

G

M

F

D

D

I

F

E

D

G

G

Q

Q

E

E

K

A

L

L

K

K

Q

D

A

S

R

R

V

V

L

L

V

I

I

V

G

G

A

L

G

G

L

L

G

G

C
|
C

A

A

A

S

Q

Q

Y

Y

L

L

G

A

V

S

A

A

G

G

V

V

G

G

A

N

M

L

T

T

L

L

V

L

D

D

F

F

D

D

T

T

V

V

E

S

L

L

S

S

N

N

L

L

Q

Q

R
|
R

Q

Q

V

T

L

L

H

H

R

S

D

D

N

A

R

T

I

V

G

G

M

Q

L

P

K

K

V

V

E

E

S

S

A

A

R

R

L

D

A

A

L

L

N

T

E

R

L

I

N

N

P

P

H

H

V

I

Q

A

I

I

N

T

P

P

I

V

G

N

R

A

V

L

L

L

D

D

P

A

E

E

M

L

D

A

A

A

L

L

V

I

A

A

D

E

H

H

S

D

L

L

V

V

L

L

D

D

C
|
C

T

T

D
|
D

N

N

V

V

A

A

V

V

R

R

Q

N

Q

Q

L

L

N

N

R

A

A

G

C
|
C

F

F

A

A

H

A

K

K

V

V

P

P

L

L

V

V

S

S

A

G

A

A

I

I

R

R

M

M

E

E

G

G

M

Q

I

I

T

T

V

V

F

F

D

T

Y

Y

A

Q

G

D

D

G

S

E

P

P

C
|
C

Y

Y

Q

R

C
|
C

F

L

A

S

S

R

L

L

F

F

G

G

E

E

Q

N

Q

A

L

L

T

T

C
|
C

V

V

E

E

S

A

G

G

I

V

L

M

A

A

P

P

V

L

V

I

G

G

L

V

M

I

G

G

C

S

L

L

Q

Q

A

A

V

M

E

E

A

A

I

I

K

K

L

M

I

L

A

A

G

G

M

Y

G

G

K

K

P

P

L

A

L

S

G

G

R

K

L

I

L

V

M

M

V

Y

D

D

A

A

M

M

T

T

M
x
C

E

Q

M

F

R

R

Q

E

M

M

I

K

L

L

P

M

R

R

Q

N

P

P

N

G

C
|
C

P

E

V

V

C
|
C

G

G

R14 (= R16) mutation R->A,K: No effect.; mutation to A: No activity; when associated with A-73.
C44 (= C46) mutation to A: No effect.
R73 (= R75) mutation to A: No effect. No activity; when associated with A-14.; mutation to K: Substantially reduced activity.
C128 (= C130) mutation to A: No effect.; mutation to Y: No activity.
D130 (= D132) mutation to A: No activity.; mutation to E: Substantially reduced activity.
C142 (= C144) mutation to A: No effect.
C172 (= C174) mutation to A: No zinc bound and no enzyme activity.
C175 (= C177) mutation to A: No zinc bound and no enzyme activity.
C187 (= C189) mutation to A: No effect.
C231 (≠ M233) mutation to A: No effect.
C244 (= C246) mutation to A: No zinc bound and almost no enzyme activity.
C247 (= C249) mutation to A: No zinc bound and almost no enzyme activity.

1jwbB Structure of the covalent acyl-adenylate form of the moeb-moad protein complex (see paper)
61% identity, 96% coverage: 6:250/254 of query aligns to 3:240/240 of 1jwbB

query
sites
1jwbB

L

L

S

S

D

D

T

Q

E

E

L

M

L

L

R

R

Y

Y

S

N

R
|
R

Q

Q

I

I

S

I

I

L

K

R

S

G

M

F

D

D

I

F

E

D

G

G

Q

Q

E

E

K

A

L

L

K

K

Q

D

A

S

R

R

V

V

L

L

V

I

I

V

G
|
G

A

L

G
|
G

L

L

G

G

C

C

A

A

A

S

Q

Q

Y

Y

L

L

G

A

V

S

A

A

G

G

V

V

G

G

A

N

M

L

T

T

L

L

V

L

D
|
D

F
|
F

D

D

T

T

V

V

E

S

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

V

T

L

L

H

H

R

S

D

D

N

A

R

T

I

V

G

G

M

Q

L

P

K
|
K

V

V

E

E

S

S

A

A

R

R

L

D

A

A

L

L

N

T

E

R

L

I

N

N

P

P

H

H

V

I

Q

A

I

I

N

T

P

P

I

V

G

N

R

A

V
x
L

L

L

D

D

P

A

E

E

M

L

D

A

A

A

L

L

V

I

A

A

D

E

H

H

S

D

L

L

V

V

L

L

D

D

C
|
C

T
|
T

D
|
D

N
|
N

V

V

A

A

V
|
V

R

R

Q

N

Q

Q

L

L

N

N

R

A

A

G

C

C

F

F

A

A

H

A

K

K

V

V

P

P

L

L

V

V

S

S

A

G

A

A

I

I

R

R

M

M

E

E

G

G

M

Q

I

I

T

T

V

V

F

F

D

T

Y

Y

A

Q

G

D

D

G

S

E

P

P

C
|
C

Y

Y

Q

R

C

C

F

L

A

S

S

R

L

L

F

F

G

G

E

-

Q

-

L

-

T

-

C

-

V

-

E

E

S

A

G

G

I

V

L

M

A

A

P

P

V

L

V

I

G

G

L

V

M

I

G

G

C

S

L

L

Q

Q

A

A

V

M

E

E

A

A

I

I

K

K

L

M

I

L

A

A

G

G

M

Y

G

G

K

K

P

P

L

A

L

S

G

G

R

K

L

I

L

V

M

M

V

Y

D

D

A

A

M

M

T

T

M

C

E

Q

M

F

R

R

Q

E

M

M

I

K

L

L

P

M

R

R

Q

N

P

P

N

G

C
|
C

P

E

V

V

C
|
C

G

G

active site: R13 (= R16), D129 (= D132)
binding adenosine monophosphate: G37 (= G40), G39 (= G42), G40 (= G43), D61 (= D64), F62 (= F65), K85 (= K88), L108 (≠ V111), C127 (= C130), T128 (= T131), D129 (= D132), N130 (= N133), V133 (= V136)
binding zinc ion: C171 (= C174), C236 (= C246), C239 (= C249)

1jw9B Structure of the native moeb-moad protein complex (see paper)
61% identity, 96% coverage: 6:250/254 of query aligns to 3:240/240 of 1jw9B

query
sites
1jw9B

L

L

S

S

D

D

T

Q

E

E

L

M

L

L

R

R

Y

Y

S

N

R
|
R

Q

Q

I

I

S

I

I

L

K

R

S

G

M

F

D

D

I

F

E

D

G

G

Q

Q

E

E

K

A

L

L

K

K

Q

D

A

S

R

R

V

V

L

L

V

I

I

V

G

G

A

L

G

G

L

L

G

G

C

C

A

A

A

S

Q

Q

Y

Y

L

L

G

A

V

S

A

A

G

G

V

V

G

G

A

N

M

L

T

T

L

L

V

L

D

D

F

F

D

D

T

T

V

V

E

S

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

V

T

L

L

H

H

R

S

D

D

N

A

R

T

I

V

G

G

M

Q

L

P

K

K

V

V

E

E

S

S

A

A

R

R

L

D

A

A

L

L

N

T

E

R

L

I

N

N

P

P

H

H

V

I

Q

A

I

I

N

T

P

P

I

V

G

N

R

A

V

L

L

L

D

D

P

A

E

E

M

L

D

A

A

A

L

L

V

I

A

A

D

E

H

H

S

D

L

L

V

V

L

L

D

D

C

C

T

T

D
|
D

N

N

V

V

A

A

V

V

R

R

Q

N

Q

Q

L

L

N

N

R

A

A

G

C

C

F

F

A

A

H

A

K

K

V

V

P

P

L

L

V

V

S

S

A

G

A

A

I

I

R

R

M

M

E

E

G

G

M

Q

I

I

T

T

V

V

F

F

D

T

Y

Y

A

Q

G

D

D

G

S

E

P

P

C
|
C

Y

Y

Q

R

C

C

F

L

A

S

S

R

L

L

F

F

G

G

E

-

Q

-

L

-

T

-

C

-

V

-

E

E

S

A

G

G

I

V

L

M

A

A

P

P

V

L

V

I

G

G

L

V

M

I

G

G

C

S

L

L

Q

Q

A

A

V

M

E

E

A

A

I

I

K

K

L

M

I

L

A

A

G

G

M

Y

G

G

K

K

P

P

L

A

L

S

G

G

R

K

L

I

L

V

M

M

V

Y

D

D

A

A

M

M

T

T

M

C

E

Q

M

F

R

R

Q

E

M

M

I

K

L

L

P

M

R

R

Q

N

P

P

N

G

C
|
C

P

E

V

V

C
|
C

G

G

active site: R13 (= R16), D129 (= D132)
binding zinc ion: C171 (= C174), C236 (= C246), C239 (= C249)

1jwaB Structure of the atp-bound moeb-moad protein complex (see paper)
58% identity, 93% coverage: 6:242/254 of query aligns to 3:217/217 of 1jwaB

query
sites
1jwaB

L

L

S

S

D

D

T

Q

E

E

L

M

L

L

R

R

Y

Y

S

N

R
|
R

Q

Q

I

I

S

I

I

L

K

R

S

G

M

F

D

D

I

F

E

D

G

G

Q

Q

E

E

K

A

L

L

K

K

Q

D

A

S

R

R

V

V

L

L

V

I

I

V

G

G

A

L

G
|
G

L

L

G

G

C

C

A

A

A

S

Q

Q

Y

Y

L

L

G

A

V

S

A

A

G

G

V

V

G

G

A

N

M

L

T

T

L

L

V

L

D
|
D

F
|
F

D

D

T

T

V

V

E

S

L

L

S

S

N

N

L

L

Q

Q

R
|
R

Q

Q

V

T

L

L

H

H

R

S

D

D

N

A

R

T

I

V

G

G

M

Q

L

P

K
|
K

V

V

E

E

S

S

A

A

R

R

L

D

A

A

L

L

N

T

E

R

L

I

N

N

P

P

H

H

V

I

Q

A

I

I

N

T

P

P

I

V

G

N

R

A

V
x
L

L

L

D

D

P

A

E

E

M

L

D

A

A

A

L

L

V

I

A

A

D

E

H

H

S

D

L

L

V

V

L

L

D

D

C

C

T

T

D
|
D

N
|
N

V

V

A

A

V
|
V

R

R

Q

N

Q

Q

L

L

N

N

R

A

A

G

C

C

F

F

A

A

H

A

K

K

V

V

P

P

L

L

V

V

S

S

A

G

A

A

I

I

R

R

M

M

E

E

G

G

M

Q

I

I

T

T

V

V

F

F

D

T

Y

Y

A

-

G

-

D

-

S

-

P

-

C

-

Y

-

Q

-

C

-

F

-

A

-

S

-

L

-

F

-

G

-

E

-

Q

-

L

-

T

-

C

-

V

-

E

E

S

A

G

G

I

V

L

M

A

A

P

P

V

L

V

I

G

G

L

V

M

I

G

G

C

S

L

L

Q

Q

A

A

V

M

E

E

A

A

I

I

K

K

L

M

I

L

A

A

G

G

M

Y

G

G

K

K

P

P

L

A

L

S

G

G

R

K

L

I

L

V

M

M

V

Y

D

D

A

A

M

M

T

T

M

C

E

Q

M

F

R

R

Q

E

M

M

I

K

L

L

P

M

R

R

active site: R13 (= R16), D129 (= D132)
binding adenosine-5'-triphosphate: G39 (= G42), G40 (= G43), D61 (= D64), F62 (= F65), R72 (= R75), K85 (= K88), L108 (≠ V111), D129 (= D132), N130 (= N133), V133 (= V136)

Q9VLJ8 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin activating enzyme 4; EC 2.7.7.80; EC 2.8.1.11 from Drosophila melanogaster (Fruit fly) (see paper)
45% identity, 97% coverage: 6:251/254 of query aligns to 64:311/453 of Q9VLJ8

query
sites
Q9VLJ8

L

L

S

T

D

N

T

D

E

D

L

I

L

A

R

R

Y

Y

S

S

R

R

Q

Q

I

L

S

I

I

L

K

P

S

D

M

F

D

G

I

V

E

Q

G

G

Q

Q

E

L

K

K

L

L

K

K

Q

N

A

S

R

S

V

V

L

L

V

I

I

V

G

G

A

L

G

G

L

L

G

G

C

C

A

P

A

A

Q

Q

Y

Y

L

L

G

A

V

A

A

A

G

G

V

C

G

G

A

H

M

L

T

G

L

L

V

V

D

D

F

Y

D

D

T

E

V

V

E

E

L

R

S

S

N

N

L

F

Q

H

R

R

Q

Q

V

I

L

L

H

H

R

S

D

E

N

D

R

R

I

C

G

G

M

M

L

S

K

K

V

A

E

E

S

S

A

A

R

R

L

I

A

A

L

L

N

L

E

E

L

L

N

N

P

P

H

H

V

C

Q

E

I

I

N

Q

P

C

I

H

G

S

R

R

V

M

L

L

D

Y

D

P

P

H

E

N

M

A

D

M

A

H

L

I

V

I

A

R

D

G

H

Y

S

D

L

V

V

V

L

L

D

D

C

C

T

T

D

D

N

N

V

V

A

P

V

T

R

R

Q

Y

Q

L

L

L

N

S

R

D

A

A

C

C

F

V

A

M

H

L

K

S

V

K

P

P

L

L

V

V

S

S

A

G

A

S

A

A

I

L

R

K

M

M

E

D

G

G

M

Q

I

L

T

T

V

V

F

Y

D

N

Y

Y

A

A

G

-

D

N

S

G

P

P

C

C

Y

Y

Q

R

C

C

F

I

A

F

S

P

L

V

-

P

F

P

G

P

E

E

Q

A

Q

V

L

T

T

N

C

C

V

G

E

D

S

G

G

G

I

V

L

L

A

G

P

A

V

V

V

T

G

G

L

T

M

I

G

G

C

A

L

M

Q

Q

A

A

V

L

E

E

A

A

I

I

K

K

L

V

I

I

A

V

G

G

M

M

G

G

K

D

P

V

L

L

L

A

G

G

R

R

L

L

M

I

V

F

D

D

A

G

M

G

T

S

M

G

E

V

M

F

R

R

Q

N

M

I

-

R

I

I

L

R

P

S

R

K

Q

R

P

P

N

N

C

C

P

H

V

M

C

C

G

S

G

A

Sites not aligning to the query:

62 modified: Phosphothreonine

O59954 Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; EC 2.7.7.80; EC 2.8.1.11 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
45% identity, 98% coverage: 6:254/254 of query aligns to 61:324/482 of O59954

query
sites
O59954

L

L

S

H

D

G

T

E

E

E

L

Y

L

R

R

R

Y

Y

S

G

R

R

Q

Q

I

M

S

I

I

V

K

P

S

Q

M

F

D

G

I

L

E

Q

G
|
G

Q

Q

E

L

K

K

L

L

K

R

Q

D

A

A

R

K

V

V

L

L

V

I

I

V

G

G

A

A

G

G

L

L

G
|
G

C

C

A

P

A

A

Q

L

Y

Y

L

L

G

A

V

G

A

A

G

G

V

V

G

G

A

T

M

I

T

G

L

L

V

V

D

D

F

G

D

D

T

T

V

V

E

E

L

A

S

S

N

N

L

L

Q

H

R
|
R

Q

Q

V

V

L

L

H

H

R

R

D

S

N

R

R

N

I

V

G

G

M

K

L

L

K

K

V

V

E

D

S

S

A

A

R

I

L

E

A

Y

L

L

N

R

E

E

L

L

N

N

P

P

H

H

V

P

Q

T

I

Y

N

I

P

A

I

H

G

Q

R

A

V

H

L

L

D

T

D

P

P

R

E

E

M

A

D

P

A

D

L

I

V

F

A

K

D

D

H

Y

S

D

L

L

V

I

L

L

D

D

C
|
C

T

T

D

D

N

N

V

P

A

A

V

T

R

R

Q

Y

Q

L

L

I

N

S

R

D

A

T

C

A

F

V

A

L

H

L

K

G

V

K

P

P

L

L

V

V

S

S

A

A

A

S

A

A

I

L

R

R

M

T

E
|
E

G

G

M

Q

I

L

T

M

V

V

F

L

D

N

Y

N

-

P

-

Q

-

P

A

P

G

G

D

D

S

K

-

T

-

G

P

P

C

C

Y

Y

Q

R

C

C

-

V

F

F

A

P

S

K

L

P

F

P

G

P

E

A

Q

N

Q

S

L

V

T

T

-

S

C

C

V

A

E

D

S

G

G

G

I

I

L

L

A

G

P

P

V

V

G
|
G

L

T

M

M

G

G

C

V

L

L

Q

Q

A

A

V

S

E

E

A

A

I

I

K

K

L

V

I

L

A

T

G

S

M

A

G

G

K

E

P

S

L

V

L

E

G

A

-

T

-

P

R

S

L

L

M

I

V

F

D

S

A

A

M

Y

T

S

M

S

-

P

E

Q

M

F

R

R

Q

T

M

I

I

K

L

L

-

R

P

S

R

R

Q

R

P

P

N

N

C

C

P

A

V

V

C

C

G

S

G

A

-

E

A

A

T

T

V

V

G82 (= G27) mutation to D: In cnxF21ts and cnxF24ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.
G100 (= G45) mutation to S: In cnxF1285; impairs molybdopterin biosynthesis.
R130 (= R75) mutation to Q: In cnxF200; impairs molybdopterin biosynthesis.
C185 (= C130) mutation to Y: In cnxF472; impairs molybdopterin biosynthesis.
E215 (= E160) mutation to K: In cnxF119; impairs molybdopterin biosynthesis.
G264 (= G200) mutation to S: In cnxF142ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.

P30138 Sulfur carrier protein ThiS adenylyltransferase; EC 2.7.7.73 from Escherichia coli (strain K12) (see 3 papers)
42% identity, 98% coverage: 6:253/254 of query aligns to 1:247/251 of P30138

query
sites
P30138

L

M

S

N

D

D

T

R

E

D

L

F

L

M

R

R

Y

Y

S

S

R

R

Q

Q

I

I

S

L

I

L

K

D

S

D

M

I

D

A

I

L

E

D

G

G

Q

Q

E

Q

K

K

L

L

K

L

Q

D

A

S

R

Q

V

V

L

L

V

I

I

I

G

G

A

L

G

G

L

L

G

G

C

T

A

P

A

A

Q

L

Y

Y

L

L

G

A

V

G

A

A

G

G

V

V

G

G

A

T

M

L

T

V

L

L

V

A

D

D

F

D

D

D

T

D

V

V

E

H

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

V

I

L

L

H

F

R

T

D

T

N

E

R

D

I

I

G

D

M

R

L

P

K

K

V

S

E

Q

S

V

A

S

R

Q

L

Q

A

R

L

L

N

T

E

Q

L

L

N

N

P

P

H

D

V

I

Q

Q

I

L

N

T

P

A

I

L

G

Q

R

Q

V

R

L

L

D

T

D

G

P

E

E

A

M

L

D

K

A

D

L

A

V

V

A

A

D

R

H

A

S

D

L

V

V

V

L

L

D

D

C

C

T

T

D

D

N

N

V

M

A

A

V

T

R

R

Q

Q

Q

E

L

I

N

N

R

A

A

A

C

C

F

V

A

A

H

L

K

N

V

T

P

P

L

L

V

I

S

T

A

A

A

S

A

A

I

V

R

G

M

F

E

G

G

G

M

Q

I

L

T

M

V

V

F

L

D

T

Y

P

A

P

G

W

D

E

S

Q

P

G

C
|
C

Y

Y

Q

R

C
|
C

F

L

A
x
W

S

P

L

D

F

N

G

Q

E

E

Q

P

Q

E

L

R

T

N

C
|
C

V

R

E

T

S

A

G

G

I

V

L

V

A

G

P

P

V

V

G

G

L

V

M

M

G

G

C

T

L

L

Q

Q

A

A

V

L

E

E

A

A

I

I

K

K

L

L

I

L

A

S

G

G

M

I

G

E

K

T

P

P

L

-

L

A

G

G

R

E

L

L

L

R

M

L

V

F

D

D

A

G

M

K

T

S

M

S

E

Q

M

W

R

R

Q

S

M

L

I

A

L

L

P

R

R

R

Q

A

P

S

N

G

C
|
C

P

P

V

V

C
|
C

G

G

A

S

T

N

C169 (= C174) binding
C172 (= C177) binding
W174 (≠ A179) mutation to A: No adenylation of ThiS.
C184 (= C189) mutation to S: No cross-link formed with ThiS. No effect on ThiS thiocarboxylate formation in vitro. Does not support growth.
C240 (= C246) binding
C243 (= C249) binding

1zfnA Structural analysis of escherichia coli thif (see paper)
42% identity, 96% coverage: 6:250/254 of query aligns to 1:244/244 of 1zfnA

query
sites
1zfnA

L

M

S

N

D

D

T

R

E

D

L

F

L

M

R

R

Y

Y

S

S

R
|
R

Q

Q

I

I

S

L

I

L

K

D

S

D

M

I

D

A

I

L

E

D

G

G

Q

Q

E

Q

K

K

L

L

K

L

Q

D

A

S

R

Q

V

V

L

L

V

I

I
|
I

G
|
G

A

L

G
|
G

L

L

G

G

C

T

A

P

A

A

Q

L

Y

Y

L

L

G

A

V

G

A

A

G

G

V

V

G

G

A

T

M

L

T

V

L

L

V

A

D
|
D

F

D

D

D

T

D

V

V

E

H

L

L

S

S

N

N

L

L

Q

Q

R
|
R

Q
|
Q

V

I

L

L

H

F

R

T

D

T

N

E

R

D

I

I

G

D

M

R

L

P

K
|
K

V

S

E

Q

S

V

A

S

R

Q

L

Q

A

R

L

L

N

T

E

Q

L

L

N

N

P

P

H

D

V

I

Q

Q

I

L

N

T

P

A

I

L

G

Q

R

Q

V

R

L

L

D

T

D

G

P

E

E

A

M

L

D

K

A

D

L

A

V

V

A

A

D

R

H

A

S

D

L

V

V

V

L

L

D

D

C

C

T
|
T

D
|
D

N

N

V

M

A

A

V
x
T

R

R

Q

Q

Q

E

L

I

N

N

R

A

A

A

C

C

F

V

A

A

H

L

K

N

V

T

P

P

L

L

V

I

S

T

A

A

A

S

A

A

I

V

R

G

M

F

E

G

G

G

M

Q

I

L

T

M

V

V

F

L

D

T

Y

P

A

P

G

W

D

E

S

Q

P

G

C
|
C

Y

Y

Q

R

C
|
C

F

L

A

W

S

P

L

D

F

N

G

Q

E

E

Q

P

Q

E

L

R

T

N

C

C

V

R

E

T

S

A

G

G

I

V

L

V

A

G

P

P

V

V

G

G

L

V

M

M

G

G

C

T

L

L

Q

Q

A

A

V

L

E

E

A

A

I

I

K

K

L

L

I

L

A

S

G

G

M

I

G

E

K

T

P

P

L

-

L

A

G

G

R

E

L

L

L

R

M

L

V

F

D

D

A

G

M

K

T

S

M

S

E

Q

M

W

R

R

Q

S

M

L

I

A

L

L

P

R

R

R

Q

A

P

S

N

G

C
|
C

P

P

V

V

C
|
C

G

G

active site: R11 (= R16), D127 (= D132)
binding adenosine-5'-triphosphate: I34 (= I39), G35 (= G40), G37 (= G42), G38 (= G43), D59 (= D64), R70 (= R75), Q71 (= Q76), K83 (= K88), T126 (= T131), D127 (= D132), T131 (≠ V136)
binding zinc ion: C169 (= C174), C172 (= C177), C240 (= C246), C243 (= C249)

O95396 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; MPT synthase sulfurylase; EC 2.7.7.80; EC 2.8.1.11 from Homo sapiens (Human) (see 5 papers)
46% identity, 89% coverage: 6:231/254 of query aligns to 55:281/460 of O95396

query
sites
O95396

L

L

S

S

D

R

T

D

E

E

L

I

L

L

R

R

Y

Y

S

S

R

R

Q

Q

I

L

S

V

I

L

K

P

S

E

M

L

D

G

I

V

E

H

G

G

Q

Q

E

L

K

R

L

L

K

G

Q

T

A

A

R

C

V

V

L

L

V

I

I

V

G

G

A

C

G

G

L

L

G

G

C

C

A

P

A

L

A

A

Q

Q

Y

Y

L

L

G

A

V

A

A

A

G

G

V

V

G

G

A

R

M

L

T

G

L

L

V

V

D

D

F

Y

D

D

T

V

V

V

E

E

L

M

S

S

N

N

L

L

Q

A

R

R

Q

Q

V

V

L

L

H

H

R

G

D

E

N

A

R

L

I

A

G

G

M

Q

L

A

K

K

V

A

E

F

S

S

A

A

R

A

L

A

A

S

L

L

N

R

E

R

L

L

N

N

P

S

H

A

V

V

Q

E

I

C

N

V

P

P

I

Y

G
x
T

R
x
Q

V
x
A

L
|
L

D
x
T

D
x
P

P
x
A

E
x
T

M
x
A

D
x
L

A
x
D

L
|
L

V
|
V

A
x
R

D
x
R

H
x
Y

S
x
D

L
x
V

V
|
V

L
x
A

D
|
D

C
|
C

T
x
S

D
|
D

N
|
N

V
|
V

A
x
P

V
x
T

R
|
R

Q
x
Y

Q
x
L

L
x
V

N
|
N

R
x
D

A
|
A

C
|
C

F
x
V

A
x
L

H
x
A

K
x
G

V
x
R

P
|
P

L
|
L

V
|
V

S
|
S

A
|
A

A
x
S

A
|
A

I
x
L

R
|
R

M
x
F

E
|
E

G
|
G

M
x
Q

I
|
I

T
|
T

V
|
V

F
x
Y

D
x
H

Y
|
Y

A
x
D

G
|
G

D

-

S
x
G

P
|
P

C
|
C

Y
|
Y

Q
x
R

C
|
C

-
x
I

F
|
F

A
x
P

S
x
Q

L
x
P

F
x
P

G
x
P

E
x
A

Q
x
E

Q
x
T

L
x
V

T
|
T

-
x
N

C
|
C

V

A

E

D

S

G

G

G

I

V

L

L

A

G

P

V

V

V

V

T

G

G

L

V

M

L

G

G

C

C

L

L

Q

Q

A

A

V

L

E

E

A

V

I

L

K

K

L

I

I

A

A

A

G

G

M

L

G

G

K

P

P

S

L

Y

L

S

G

G

R

S

L

L

M

L

V

F

D

D

A

A

M

L

158:238 (vs. 109:188, 40% identical) Interaction with NFS1
C239 (= C189) mutation to A: Impairs sulfurtransferase activity.

Sites not aligning to the query:

316 modified: Disulfide link with 324; C→A: Does not affect sulfurtransferase activity.
324 modified: Disulfide link with 316; C→A: Does not affect sulfurtransferase activity.
365 C→A: Does not affect sulfurtransferase activity.
412 active site, Cysteine persulfide intermediate; for sulfurtransferase activity; modified: Cysteine persulfide; C→A: Abolishes sulfurtransferase activity.
413 K→R: Does not affect sulfurtransferase specificity and activity.
414 L→K: Does not affect sulfurtransferase specificity and activity.
415 G→A: Does not affect sulfurtransferase specificity and activity.
416 N→V: Does not affect sulfurtransferase specificity and activity.
417 D→R: Results in 470-fold increased activity.; D→T: Results in 90-fold increased activity.
458 P→G: Does not affect sulfurtransferase specificity and activity.
460 Y→A: Does not affect sulfurtransferase specificity and activity.

1zud3 Structure of this-thif protein complex (see paper)
40% identity, 97% coverage: 6:251/254 of query aligns to 1:240/240 of 1zud3

query
sites
1zud3

L

M

S

N

D

D

T

R

E

D

L

F

L

M

R

R

Y

Y

S

S

R
|
R

Q

Q

I

I

S
x
L

I

L

K
x
D

S

D

M

I

D

A

I

L

E

D

G

G

Q

Q

E

Q

K

K

L

L

K

L

Q

D

A

S

R

Q

V

V

L

L

V

I

I

I

G

G

A

L

G

G

L

L

G

G

C

T

A

P

A

A

Q

L

Y

Y

L

L

G

A

V

G

A

A

G

G

V

V

G

G

A

T

M

L

T

V

L

L

V

A

D

D

F

D

D

D

T

D

V

V

E

H

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

V

I

L

L

H

F

R

T

D

T

N

E

R

D

I

I

G

D

M

R

L

P

K

K

V

S

E

Q

S

V

A

S

R

Q

L

Q

A

R

L

L

N

T

E

Q

L

L

N

N

P

P

H

D

V

I

Q

Q

I

L

N

T

P

A

I

L

G

Q

R

Q

V

R

L

L

D

T

D

G

P

E

E

A

M

L

D

K

A

D

L

A

V

V

A

A

D

R

H

A

S

D

L

V

V

V

L

L

D

D

C

C

T

T

D
|
D

N

N

V

M

A

A

V

T

R

R

Q

Q

Q

E

L

I

N

N

R

A

A

A

C

C

F

V

A

A

H

L

K

N

V

T

P

P

L

L

V

I

S

T

A

A

A

S

A

A

I

V

R

G

M

F

E

G

G

G

M

Q

I

L

T

M

V

V

F

L

D

T

Y

P

A

P

G

W

D

E

S

Q

P

G

C
|
C

Y

Y

Q

R

C

C

F

-

A

-

S

-

L

L

F

W

G

P

E

D

Q

N

Q

Q

L

E

T

P

C

T

V

A

E

G

S

V

G

V

I

G

L

P

A

V

P

V

V

G

V

V

G

-

L

-

M

M

G

G

C

T

L

L

Q

Q

A

A

V

L

E

E

A

A

I

I

K

K

L

L

I

L

A

S

G

G

M

I

G

E

K

T

P

P

L

-

L

A

G

G

R

E

L

L

L

R

M

L

V

F

D

D

A

G

M

K

T

S

M

S

E

Q

M

W

R

R

Q

S

M

L

I

A

L

L

P

R

R

R

Q

A

P

S

N

G

C
|
C

P

P

V

V

C
|
C

G

G

active site: R11 (= R16), D127 (= D132)
binding calcium ion: L14 (≠ S19), D16 (≠ K21)
binding zinc ion: C169 (= C174), C235 (= C246), C238 (= C249)

P38820 Adenylyltransferase and sulfurtransferase UBA4; Needs CLA4 to survive protein 3; Ubiquitin-like protein activator 4; EC 2.7.7.-; EC 2.8.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
38% identity, 98% coverage: 3:250/254 of query aligns to 36:290/440 of P38820

query
sites
P38820

D

D

D

Y

I

P

L

L

S

S

D

L

T

E

E

E

L

Y

L

Q

R

R

Y

Y

S

G

R

R

Q

Q

I

M

S

I

I

V

K

E

S

E

M

T

D

G

-

G

I

V

E

A

G

G

Q

Q

E

V

K

K

L

L

K

K

Q

N

A

T

R

K

V

V

L

L

V

V

I

V

G

G

A

A

G

G

L

L

G

G

C

C

A

P

A

A

A

L

Q

P

Y

Y

L

L

G

A

V

G

A

A

G

G

V

V

G

G

A

Q

M

I

T

G

L

I

V

V

D

D

F

N

D

D

T

V

V

V

E

E

L

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

V

V

L

L

H

H

R

D

D

S

N

S

R

R

I

V

G

G

M

M

L

L

K

K

V

C

E

E

S

S

A

A

R

R

L

Q

A

Y

L

I

N

T

E

K

L

L

N

N

P

P

H

H

V

I

Q

N

I

V

N

V

P

T

I

Y

G

P

R

V

V

R

L

L

D

N

D

S

P

S

E

N

M

A

D

F

A

D

L

I

V

F

A

K

D

G

H

Y

S

N

L

Y

V

I

L

L

D

D

C

C

T

T

D

D

N

S

V

P

A

L

V

T

R

R

Q

Y

Q

L

L

V

N

S

R

D

A

V

C

A

F

V

A

N

H

L

K

G

V

I

P

T

L

V

V

V

S

S

A

A

A

S

A

G

I

L

R

G

M

T

E

E

G

G

M

Q

I

L

T

T

V

I

F

L

D

N

Y

F

A

N

G

N

D

I

S

G

P

P

C

C

Y

Y

Q

R

C

C

F

F

A

Y

S

P

L

T

F

P

G

P

E

P

Q

P

Q

N

-

A

-

V

L

T

T

S

C
|
C

V

Q

E

E

S

G

G

G

I

V

L

I

A

G

P

P

V

C

V

I

G

G

L

L

M

V

G

G

C

T

L

M

Q

M

A

A

V

V

E

E

A

T

I

L

K

K

L

L

I

I

A

L

G

G

M

I

-

Y

G

T

K

N

P

E

L

N

L

F

G

S

R

P

L

F

L

L

M

M

V

L

D

Y

A

S

-

G

-

F

-

P

-

Q

-

S

-

L

M

R

T

T

M

F

E

K

M

M

R

R

Q

G

M

-

I

-

L

-

P

-

R

R

Q

Q

P

E

N

K

C

C

P

L

V

C

C

C

G

G

C225 (= C189) mutation C->A,S: Abolishes adenylyltransferase activity but not sulfurtransferase activity.

Sites not aligning to the query:

397 mutation C->A,S: Abolishes sulfurtransferase activity but not adenylyltransferase activity.

6yubB Crystal structure of uba4 from chaetomium thermophilum (see paper)
43% identity, 84% coverage: 2:214/254 of query aligns to 3:212/289 of 6yubB

query
sites
6yubB

T

T

D

Q

D

K

I

S

L

L

S

S

D

K

T

E

E

E

L

I

L

E

R

R

Y

Y

S

S

R

R

Q

Q

I

M

S

I

I

V

K

P

S

G

M

M

D

G

I

K

E

E

G

G

Q

Q

E

L

K

R

L

L

K

M

Q

N

A

A

R

K

V

V

L

L

V

I

I

I

G

G

A

A

G

G

L

L

G

G

C

C

A

P

A

A

Q

Q

Y

Y

L

L

G

A

V

G

A

A

G

G

V

V

G

G

A

T

M

I

T

G

L

I

V

V

D

D

F

G

D

D

T

S

V

V

E

E

L

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

V

V

L

A

H

H

R

A

D

T

N

K

R

R

I

V

G

G

M

M

L

L

K

K

V

V

E

D

S

S

A

L

R

I

L

T

A

H

L

L

N

I

E

E

L

I

N

N

P

P

H

L

V

P

Q

V

I

Y

N

V

P

P

I

Y

G

R

R

F

V

D

L

L

D

T

D

P

P

Q

E

N

M

A

D

A

A

Q

L

I

V

I

A

K

D

P

H

W

S

D

L

V

V

I

L

L

D

D

C

C

T

T

D

D

N

N

V

P

A

A

V

T

R

R

Q

Y

Q

L

L

I

N

S

R

D

A

V

C

C

F

V

A

L

H

L

K

G

V

K

P

P

L

L

V

V

S

S

A

A

A

S

I

V

R

Q

M

K

E

S

G

G

M

Q

I

L

T

I

V

V

F

L

D

N

-

C

-

P

-

T

-

P

-

Q

-

G

-

V

Y

N

A

K

G

K

D

A

S

A

P

P

C
|
C

Y

Y

Q

R

C
|
C

F

-

A

-

S

-

L

-

F

-

G

-

E

-

Q

-

L

-

T

-

C

C

V

F

E

K

S

-

G

G

I

I

L

M

A

G

P

P

V

V

G

G

L

M

M

M

G

G

C

V

L

A

Q

Q

A

A

V

G

E

E

A

A

I

I

K

K

L

I

I

L

binding zinc ion: C184 (= C174), C187 (= C177)

Sites not aligning to the query:

binding zinc ion: 265

6yubA Crystal structure of uba4 from chaetomium thermophilum (see paper)
43% identity, 84% coverage: 2:214/254 of query aligns to 2:213/423 of 6yubA

query
sites
6yubA

T

T

D

Q

D

K

I

S

L

L

S

S

D

K

T

E

E

E

L

I

L

E

R

R

Y

Y

S

S

R

R

Q

Q

I

M

S

I

I

V

K

P

S

G

M

M

D

G

I

K

E

E

G

G

Q

Q

E

L

K

R

L

L

K

M

Q

N

A

A

R

K

V

V

L

L

V

I

I

I

G

G

A

A

G

G

L

L

G

G

C

C

A

P

A

A

Q

Q

Y

Y

L

L

G

A

V

G

A

A

G

G

V

V

G

G

A

T

M

I

T

G

L

I

V

V

D

D

F

G

D

D

T

S

V

V

E

E

L

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

V

V

L

A

H

H

R

A

D

T

N

K

R

R

I

V

G

G

M

M

L

L

K

K

V

V

E

D

S

S

A

L

R

I

L

T

A

H

L

L

N

I

E

E

L

I

N

N

P

P

H

L

V

P

Q

V

I

Y

N

V

P

P

I

Y

G

R

R

F

V

D

L

L

D

T

D

P

P

Q

E

N

M

A

D

A

A

Q

L

I

V

I

A

K

D

P

H

W

S

D

L

V

V

I

L

L

D

D

C

C

T

T

D

D

N

N

V

P

A

A

V

T

R

R

Q

Y

Q

L

L

I

N

S

R

D

A

V

C

C

F

V

A

L

H

L

K

G

V

K

P

P

L

L

V

V

S

S

A

A

A

S

I

V

R

Q

M

K

E

S

G

G

M

Q

I

L

T

I

V

V

F

L

D

N

-

C

-

P

-

T

-

P

-

Q

-

G

-

V

Y

N

A

K

G

K

D

A

S

A

P

P

C
|
C

Y

Y

Q

R

C
|
C

F

-

A

-

S

-

L

-

F

-

G

-

E

-

Q

-

L

-

T

-

C

C

V

F

E

K

S

K

-

P

G

G

I

I

L

M

A

G

P

P

V

V

G

G

L

M

M

M

G

G

C

V

L

A

Q

Q

A

A

V

G

E

E

A

A

I

I

K

K

L

I

I

L

binding zinc ion: C183 (= C174), C186 (= C177)

Sites not aligning to the query:

binding zinc ion: 266, 269

D4GSF3 SAMP-activating enzyme E1; Ubiquitin-like activating enzyme of archaea; Ubl-activating enzyme; EC 2.7.7.- from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
42% identity, 96% coverage: 6:250/254 of query aligns to 5:249/270 of D4GSF3

query
sites
D4GSF3

L

L

S

D

D

A

T

T

E

Q

L

L

L

D

R

R

Y

Y

S

S

R

R

Q

H

I

I

S

I

I

M

K

D

S

E

M

V

D

G

I

P

E

E

G

G

Q

Q

E

G

K

R

L

L

K

L

Q

S

A

S

R

R

V

V

L

V

V

V

I

V

G

G

A

A

G

G

L

L

G

G

C

A

A

P

A

A

A

I

Q

Q

Y

Y

L

L

G

A

V

A

A

V

G

G

V

V

G

G

A

E

M

L

T

V

L

V

V

V

D

D

F

D

D

D

T

V

V

V

E

E

L

R

S

S

N

N

L

L

Q

Q

R

R

Q

Q

V

V

L

V

H

H

R

C

D

D

N

D

R

D

I

V

G

G

M

T

L

P

K

K

V

A

E

E

S

S

A

A

R

A

L

A

A

F

L

V

N

R

E

G

L

L

N

N

P

P

H

D

V

V

Q

S

I

V

N

E

P

P

I

V

G

E

R

A

V

R

L

V

D

D

D

K

P

S

E

N

M

V

D

H

A

E

L

V

V

V

A

A

D

G

H

S

S

D

L

V

V

V

L

V

D

D

C

A

T

S

D

D

N

N

V

F

A

P

V

T

R

R

Q

Y

Q

L

L

L

N

N

R

D

A

V

C

C

F

R

A

F

H

E

K

G

V

I

P

P

L

L

V

V

S

H

A

G

A

A

A

I

I

Y

R

K

M

F

E

E

G

G

M

Q

I

A

T

T

V

T

F

L

D

-

Y

-

A

V

G

P

D

D

S

G

P

P

C

C

Y

Y

Q

R

C

C

F

L

A

F

S

P

L

E

F

A

G

P

E

E

Q

P

Q

G

L

T

T

V

-

P

-

D

C
|
C

V

A

E

T

S

T

G

G

I

V

L

L

A

G

P

V

V

L

V

P

G

G

L

T

M

V

G

G

C

C

L

I

Q

Q

A

A

V

T

E

E

A

A

I

M

K

K

L

L

I

L

A

L

G

D

M

E

G

G

K

E

P

A

L

L

L

D

G

G

R

R

L

L

M

F

V

Y

D

D

A

A

M

M

T

D

M

M

E

T

M

F

R

E

Q

T

M

V

I

P

L

Y

P

R

R

T

Q

N

P

P

N

D

C

C

P

P

V

V

C

C

G

G

C188 (= C189) mutation to S: Loss of activity since this mutant is not able to complement a ubaA deletion in trans to restore sampylation and tRNA thiolation.

Q72J02 Sulfur carrier protein adenylyltransferase; E1-like protein TtuC; Sulfur carrier protein MoaD adenylyltransferase; Sulfur carrier protein ThiS adenylyltransferase; Sulfur carrier protein TtuB adenylyltransferase; tRNA two-thiouridine-synthesizing protein C; EC 2.7.7.80; EC 2.7.7.73; EC 2.7.7.- from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
38% identity, 96% coverage: 6:250/254 of query aligns to 3:253/271 of Q72J02

query
sites
Q72J02

L

W

S

T

D

K

T

E

E

E

L

L

L

D

R

R

Y

Y

S

H

R

R

Q

Q

I

M

S

I

I

L

K

P

S

Q

M

V

D

G

I

P

E

E

G

G

Q

Q

E

E

K

R

L

L

K

K

Q

R

A

A

R

S

V

V

L

V

V

V

I

V

G

G

A

A

G

G

L

L

G

G

C

V

A

P

A

V

A

L

Q

Q

Y

Y

L

L

G

V

V

A

A

A

G

G

V

V

G

G

A

R

M

V

T

G

L

V

V

V

D

E

F

M

D

D

T

R

V

V

E

E

L

V

S

S

N

N

L

L

Q

H

R

R

Q

Q

V

V

L

L

H

Y

R

T

D

T

N

E

R

D

I

V

G

G

M

E

L

P

K

K

V

A

E

L

S

V

A

A

R

Q

L

K

A

R

L

L

N

Q

E

A

L

L

N

N

P

P

H

L

V

V

Q

R

I

V

N

E

P

A

I

Y

G

P

R

V

V

R

L

L

D

T

D

S

P

E

E

N

M

A

D

L

A

E

L

I

V

L

A

R

D

P

H

Y

S

D

L

L

V

V

L

V

D

D

C

A

T

S

D

D

N

N

V

F

A

P

V

T

R

R

Q

Y

Q

L

L

V

N

N

R

D

A

A

C

A

F

V

A

L

H

L

K

G

V

K

P

P

L

L

V

V

S

F

A

G

A

A

A

I

I

Y

R

Q

M

F

E

D

G

G

M

Q

I

V

T

A

V

V

F

F

D

H

Y

H

A

P

-

T

-

L

-

H

G

G

D

E

-

M

S

G

P

P

C

C

Y

Y

Q

R

C

C

F

-

A

-

S

-

L

L

F

F

G

P

E

K

Q

P

L

P

-

P

-

G

-

A

-

V

-

P

T

S

C
|
C

V

A

E

E

S

A

G

G

I

V

L

F

A

G

P

V

V

L

V

P

G

A

L

V

M

V

G

G

C

S

L

L

Q

M

A

A

V

A

E

E

A

A

I

L

K

K

L

V

I

L

A

L

G

G

M

I

G

G

K

K

P

P

L

L

L

A

G

G

R

H

L

L

M

L

V

Y

D

D

A

A

M

L

T

E

M

A

E

S

M

F

R

R

Q

K

M

L

I

T

L

V

P

R

R

R

Q

N

P

P

N

R

C

C

P

P

V

V

C

C

G

G

C192 (= C189) modified: Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in TtuB); mutation to S: Not able to form a thioester complex with TtuB.

Sites not aligning to the query:

268 C→S: Still able to form a thioester complex with TtuB.

3h9jA Crystal structure of e. Coli mccb + ampcpp + semet mcca (see paper)
27% identity, 96% coverage: 7:251/254 of query aligns to 85:339/339 of 3h9jA

query
sites
3h9jA

S

N

D

S

T

T

E

E

L

N

R

R

Y

Y

S

S

R

R

Q

N

I

F

-

L

-

H

-

Y

-

Q

S

S

I

Y

K

G

S

A

M

N

D

P

I

V

E

L

G

V

Q

Q

E

D

K

K

L

L

K

K

Q

N

A

A

R

K

V

V

L

V

V

I

I

L

G

G

A

C

G
|
G

L

I

G

G

C

N

A

H

A

V

A

S

Q

V

Y

I

L

L

G

A

V

T

A

S

G

G

V

I

G

G

A

E

M

I

T

I

L

L

V

I

D
|
D

F

N

D
|
D

T

Q

V

I

E

E

L

N

S

T

N

N

L

L

Q

T

R
|
R

Q

Q

V

V

L

L

H

F

R

S

D

E

N

D

R

D

I

V

G

G

M

K

L

N

K
|
K

V

T

E

E

S

V

A

I

R

K

L

R

A

E

L

L

N

L

E

K

L

R

N

N

P

S

H

E

V

I

Q

S

I

V

N

S

P

E

I

I

G

A

R

L

V
x
N

L
x
I

D

N

D

D

-

Y

-

T

-

D

-

L

-

H

-

K

-

V

P

P

E

E

M

A

D

D

A

I

L

W

V

V

-

V

-

S

A
|
A

D
|
D

H
|
H

S

P

L

F

V

N

L

L

D

-

C

-

T

-

D

-

N

-

V

-

A

-

V

-

R

I

Q

N

Q

W

L

V

N

N

R

K

A

Y

C

C

F

V

A

R

H

A

K

N

V

Q

P

P

L

Y

V

I

S

N

A

A

A

G

A

Y

I

V

R

N

M

D

E

I

G

A

M

V

I

F

T

G

V

P

F

L

D

Y

Y

V

A

P

G

G

D

K

S

T

P

G

C
|
C

Y

Y

Q

E

C

C

F

Q

A

K

S

K

L

E

F

N

G

I

E

D

Q

H

Q

K

L

I

T

K

C

L

V

I

E

N

S

S

-

R

-

F

-

K

-

P

G

A

I

T

L

F

A

A

P

P

V

V

V

N

G

N

L

V

M

A

G

A

C

A

L

L

Q

C

A

A

V

A

E

D

A

V

I

I

K

K

L

F

I

I

A

G

G

K

M

Y

G

S

K

E

P

P

L

L

-

S

L

L

G

N

R

K

L
x
R

L

I

M
x
G

V

I

D

W

A

S

M

D

T

E

M

I

E

K

M

I

R

H

Q

S

M

Q

I

N

L

M

P

G

R

R

Q

S

P

P

N

V

C
|
C

P

S

V

V

C
|
C

G

G

G

N

active site: R157 (= R75)
binding diphosphomethylphosphonic acid adenosyl ester: G124 (= G42), G125 (= G43), D146 (= D64), D148 (= D66), R157 (= R75), K170 (= K88), N193 (≠ V111), I194 (≠ L112), A213 (= A122), D214 (= D123), H215 (= H124)
binding zinc ion: C257 (= C174), C334 (= C246), C337 (= C249)
binding : R313 (≠ L225), G315 (≠ M227)

3h5aC Crystal structure of e. Coli mccb (see paper)
27% identity, 96% coverage: 7:251/254 of query aligns to 85:348/358 of 3h5aC

query
sites
3h5aC

S

N

D

S

T

T

E

E

L

N

R

R

Y

Y

S

S

R

R

Q

N

I

F

-

L

-

H

-

Y

-

Q

S

S

I

Y

K

G

S

A

M

N

D

P

I

V

E

L

G

V

Q

Q

E

D

K

K

L

L

K

K

Q

N

A

A

R

K

V

V

L

V

V

I

I

L

G

G

A

C

G

G

L

I

G

G

C

N

A

H

A

V

A

S

Q

V

Y

I

L

L

G

A

V

T

A

S

G

G

V

I

G

G

A

E

M

I

T

I

L

L

V

I

D

D

F

N

D

D

T

Q

V

I

E

E

L

N

S

T

N

N

L

L

Q

T

R
|
R

Q

Q

V

V

L

L

H

F

R

S

D

E

N

D

R

D

I

V

G

G

M

K

L

N

K

K

V

T

E

E

S

V

A

I

R

K

L

R

A

E

L

L

N

L

E

K

L

R

N

N

P

S

H

E

V

I

Q

S

I

V

N

S

P

E

I

I

G

A

R

L

V

N

L

I

D

N

D

D

-

Y

-

T

-

D

-

L

-

H

-

K

-

V

P

P

E

E

M

A

D

D

A

I

L

W

V

V

-

V

-

S

A

A

D

D

H

H

S

P

L

F

V

N

L

L

D

-

C

-

T

-

D

-

N

-

V

-

A

-

V

-

R

I

Q

N

Q

W

L

V

N

N

R

K

A

Y

C

C

F

V

A

R

H

A

K

N

V

Q

P

P

L

Y

V

I

S

N

A

A

A

G

A

Y

I

V

R

N

M

D

E

I

G

A

M

V

I

F

T

G

V

P

F

L

D

Y

Y

V

A

P

G

G

D

K

S

T

P

G

C
|
C

Y

Y

Q

E

C
|
C

-

Q

-

K

-

V

F

V

A

A

S

D

L

L

F

Y

G

G

-

S

-

E

-

K

-

E

-

N

-

I

E

D

Q

H

Q

K

L

I

T

K

C

L

V

I

E

N

S

S

-

R

-

F

-

K

-

P

G

A

I

T

L

F

A

A

P

P

V

V

V

N

G

N

L

V

M

A

G

A

C

A

L

L

Q

C

A

A

V

A

E

D

A

V

I

I

K

K

L

F

I

I

A

G

G

K

M

Y

G

S

K

E

P

P

L

L

-

S

L

L

G

N

R

K

L

R

L

I

M

G

V

I

D

W

A

S

M

D

T

E

M

I

E

K

M

I

R

H

Q

S

M

Q

I

N

L

M

P

G

R

R

Q

S

P

P

N

V

C
|
C

P

S

V

V

C

C

G

G

G

N

active site: R157 (= R75)
binding zinc ion: C257 (= C174), C260 (= C177), C343 (= C246)

3h5nA Crystal structure of e. Coli mccb + atp (see paper)
27% identity, 96% coverage: 7:250/254 of query aligns to 85:338/338 of 3h5nA

query
sites
3h5nA

S

N

D

S

T

T

E

E

L

N

R

R

Y

Y

S

S

R

R

Q

N

I

F

-

L

-

H

-

Y

-

Q

S

S

I

Y

K

G

S

A

M

N

D

P

I

V

E

L

G

V

Q

Q

E

D

K

K

L

L

K

K

Q

N

A

A

R

K

V

V

L

V

V

I

I

L

G

G

A

C

G
|
G

G

G

L

I

G

G

C

N

A

H

A

V

A

S

Q

V

Y

I

L

L

G

A

V

T

A

S

G

G

V

I

G

G

A

E

M

I

T

I

L

L

V

I

D
|
D

F

N

D

D

T

Q

V

I

E

E

L

N

S

T

N
|
N

L

L

Q

T

R
|
R

Q
|
Q

V

V

L

L

H

F

R

S

D

E

N

D

R

D

I

V

G

G

M

K

L

N

K
|
K

V

T

E

E

S

V

A

I

R

K

L

R

A

E

L

L

N

L

E

K

L

R

N

N

P

S

H

E

V

I

Q

S

I

V

N

S

P

E

I

I

G

A

R

L

V
x
N

L
x
I

D

N

D

D

-

Y

-

T

-

D

-

L

-

H

-

K

-

V

P

P

E

E

M

A

D

D

A

I

L

W

V

V

-

V

-

S

A
|
A

D
|
D

H
|
H

S

P

L

F

V

N

L

L

D

-

C

-

T

-

D

-

N

-

V

-

A

-

V

-

R

I

Q

N

Q

W

L

V

N

N

R

K

A

Y

C

C

F

V

A

R

H

A

K

N

V

Q

P

P

L

Y

V

I

S

N

A

A

A

G

A

Y

I

V

R

N

M

D

E

I

G

A

M

V

I

F

T

G

V

P

F

L

D

Y

Y

V

A

P

G

G

D

K

S

T

P

G

C
|
C

Y

Y

Q

E

C
|
C

F

Q

A

K

S

K

L

E

F

N

G

I

E

D

Q

H

Q

K

L

I

T

K

C

L

V

I

E

N

S

S

-

R

-

F

-

K

-

P

G

A

I

T

L

F

A

A

P

P

V

V

V

N

G

N

L

V

M

A

G

A

C

A

L

L

Q

C

A

A

V

A

E

D

A

V

I

I

K

K

L

F

I

I

A

G

G

K

M

Y

G

S

K

E

P

P

L

L

-

S

L

L

G

N

R

K

L

R

L

I

M

G

V

I

D

W

A

S

M

D

T

E

M

I

E

K

M

I

R

H

Q

S

M

Q

I

N

L

M

P

G

R

R

Q

S

P

P

N

V

C
|
C

P

S

V

V

C
|
C

G

G

active site: R157 (= R75)
binding adenosine-5'-triphosphate: G124 (= G42), D146 (= D64), N154 (= N72), R157 (= R75), Q158 (= Q76), K170 (= K88), N193 (≠ V111), I194 (≠ L112), A213 (= A122), D214 (= D123), H215 (= H124)
binding zinc ion: C257 (= C174), C260 (= C177), C334 (= C246), C337 (= C249)

3h5rA Crystal structure of e. Coli mccb + succinimide (see paper)
27% identity, 96% coverage: 7:251/254 of query aligns to 85:340/340 of 3h5rA

query
sites
3h5rA

S

N

D

S

T

T

E

E

L

N

R

R

Y

Y

S

S

R

R

Q

N

I

F

-

L

-

H

-

Y

-

Q

S

S

I

Y

K

G

S

A

M

N

D

P

I

V

E

L

G

V

Q

Q

E

D

K

K

L

L

K

K

Q

N

A

A

R

K

V

V

L

V

V

I

I

L

G

G

A

C

G

G

L
x
I

G

G

C

N

A

H

A

V

A

S

Q

V

Y

I

L

L

G

A

V

T

A

S

G

G

V

I

G

G

A

E

M

I

T

I

L

L

V

I

D

D

F

N

D

D

T

Q

V

I

E

E

L

N

S

T

N

N

L

L

Q

T

R
|
R

Q

Q

V

V

L

L

H

F

R

S

D

E

N

D

R

D

I

V

G

G

M

K

L

N

K

K

V

T

E

E

S

V

A

I

R

K

L

R

A

E

L

L

N

L

E

K

L

R

N

N

P

S

H

E

V

I

Q

S

I

V

N

S

P

E

I

I

G

A

R

L

V

N

L

I

D

N

D

D

-

Y

-

T

-

D

-

L

-

H

-

K

-

V

P

P

E

E

M

A

D

D

A

I

L

W

V

V

-

V

-
x
S

A
|
A

D

D

H

H

S

P

L

F

V

N

L

L

D

-

C

-

T

-

D

-

N

-

V

-

A

-

V

-

R

I

Q

N

Q

W

L

V

N

N

R

K

A

Y

C

C

F

V

A

R

H

A

K

N

V

Q

P

P

L

Y

V

I

S

N

A
|
A

A
x
G

A
x
Y

I

V

R

N

M

D

E

I

G

A

M
x
V

I

F

T

G

V

P

F

L

D

Y

Y

V

A

P

G

G

D

K

S

T

P

G

C
|
C

Y

Y

Q

E

C
|
C

F

Q

A

K

S

V

L

K

F

E

G

N

-

I

E

D

Q

H

Q

K

L

I

T

K

C

L

V

I

E

N

S

S

-

R

-

F

-

K

-
x
P

G

A

I

T

L

F

A

A

P

P

V

V

V

N

G

N

L

V

M

A

G

A

C

A

L

L

Q

C

A

A

V

A

E

D

A

V

I

I

K

K

L

F

I

I

A

G

G

K

M

Y

G

S

K

E

P

P

L

L

-

S

L

L

G

N

R

K

L
x
R

L

I

M
x
G

V

I

D

W

A

S

M

D

T

E

M

I

E

K

M

I

R

H

Q

S

M

Q

I

N

L

M

P

G

R

R

Q

S

P

P

N

V

C
|
C

P

S

V

V

C
|
C

G

G

G

N

active site: R157 (= R75)
binding zinc ion: C257 (= C174), C260 (= C177), C335 (= C246), C338 (= C249)
binding : I126 (≠ L44), S212 (vs. gap), A213 (= A122), A237 (= A154), G238 (≠ A155), Y239 (≠ A156), V245 (≠ M162), P280 (vs. gap), R314 (≠ L225), G316 (≠ M227)

Sites not aligning to the query:

binding : 10, 14, 26

6om4A The structure of microcin c7 biosynthetic enzyme mccb in complex with n-formylated mcca (see paper)
27% identity, 88% coverage: 28:251/254 of query aligns to 107:345/345 of 6om4A

query
sites
6om4A

Q

Q

E

D

K

K

L

L

K

K

Q

N

A

A

R

K

V

V

L

V

V

I

I

L

G

G

A

C

G
|
G

G

G

L
x
I

G

G

C

N

A

H

A

V

A

S

Q

V

Y

I

L

L

G

A

V

T

A

S

G

G

V

I

G

G

A

E

M

I

T

I

L

L

V

I

D
|
D

F

N

D

D

T

Q

V

I

E

E

L

N

S

T

N
|
N

L

L

Q

T

R
|
R

Q

Q

V

V

L

L

H

F

R

S

D

E

N

D

R

D

I

V

G

G

M

K

L

N

K
|
K

V

T

E

E

S

V

A

I

R

K

L

R

A

E

L

L

N

L

E

K

L

R

N

N

P

S

H

E

V

I

Q

S

I

V

N

S

P

E

I

I

G

A

R

L

V
x
N

L
x
I

D

N

D

D

-

Y

-

T

-

D

-

L

-

H

-

K

-

V

P

P

E

E

M

A

D

D

A

I

L

W

V

V

-

V

-
x
S

A
|
A

D
|
D

H
|
H

S

P

L

F

V

N

L

L

D

-

C

-

T

-

D

-

N

-

V

-

A

-

V

-

R

I

Q

N

Q

W

L

V

N

N

R

K

A

Y

C

C

F

V

A

R

H

A

K

N

V

Q

P

P

L

Y

V

I

S
x
N

A
|
A

A
x
G

A
x
Y

I
x
V

R

N

M

D

E

I

G

A

M

V

I

F

T

G

V

P

F

L

D

Y

Y

V

A

P

G

G

D

K

S

T

P

G

C
|
C

Y

Y

Q

E

C
|
C

-

Q

-

K

-

V

F
x
V

A
|
A

S

D

L

L

F
x
Y

G

G

-

A

-

E

-

K

-

E

-

N

-

I

E

D

Q

H

Q

K

L

I

T

K

C

L

V

I

E

N

S

S

-

R

-

F

-

K

-

P

G

A

I

T

L

F

A

A

P

P

V

V

V

N

G

N

L

V

M

A

G

A

C

A

L

L

Q

C

A

A

V

A

E

D

A

V

I

I

K

K

L

F

I

I

A

G

G

K

M

Y

G

S

K

E

P

P

L

L

-

S

L

L

G

N

R

K

L
x
R

L

I

M
x
G

V

I

D
x
W

A

S

M

D

T

E

M

I

E

K

M

I

R

H

Q

S

M
x
Q

I

N

L

M

P

G

R

R

Q

S

P

P

N

V

C
|
C

P

S

V

V

C
|
C

G

G

G

N

binding 5'-O-[(S)-amino(hydroxy)phosphoryl]adenosine: G121 (= G42), D143 (= D64), K167 (= K88), N190 (≠ V111), I191 (≠ L112), S209 (vs. gap), A210 (= A122), D211 (= D123), H212 (= H124)
binding pyrophosphate 2-: N151 (= N72), R154 (= R75), K167 (= K88)
binding zinc ion: C254 (= C174), C257 (= C177), C340 (= C246), C343 (= C249)
binding : I123 (≠ L44), S209 (vs. gap), A210 (= A122), D211 (= D123), N233 (≠ S153), A234 (= A154), G235 (≠ A155), Y236 (≠ A156), V237 (≠ I157), V261 (≠ F178), A262 (= A179), Y265 (≠ F182), R319 (≠ L225), G321 (≠ M227), W323 (≠ D229), Q332 (≠ M238)

Sites not aligning to the query:

binding : 10, 19, 23, 26

Query Sequence

>6935922 FitnessBrowser__SB2B:6935922
MTDDILSDTELLRYSRQISIKSMDIEGQEKLKQARVLVIGAGGLGCAAAQYLGVAGVGAM
TLVDFDTVELSNLQRQVLHRDNRIGMLKVESARLALNELNPHVQINPIGRVLDDPEMDAL
VADHSLVLDCTDNVAVRQQLNRACFAHKVPLVSAAAIRMEGMITVFDYAGDSPCYQCFAS
LFGEQQLTCVESGILAPVVGLMGCLQAVEAIKLIAGMGKPLLGRLLMVDAMTMEMRQMIL
PRQPNCPVCGGATV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory