SitesBLAST
Comparing 6936328 FitnessBrowser__SB2B:6936328 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5b4tA Crystal structure of d-3-hydroxybutyrate dehydrogenase from alcaligenes faecalis complexed with NAD+ and a substrate d-3- hydroxybutyrate (see paper)
51% identity, 97% coverage: 8:266/266 of query aligns to 2:260/260 of 5b4tA
- active site: G15 (= G21), N114 (= N120), S142 (= S148), Y155 (= Y161), K159 (= K165), I200 (≠ V206)
- binding (3R)-3-hydroxybutanoic acid: Q94 (= Q100), S142 (= S148), H144 (= H150), K152 (= K158), Y155 (= Y161), W187 (= W193), Q196 (= Q202)
- binding nicotinamide-adenine-dinucleotide: G11 (= G17), T13 (= T19), G15 (= G21), I16 (= I22), F36 (≠ L42), D63 (= D69), L64 (= L70), N90 (= N96), G92 (= G98), L113 (≠ I119), I140 (= I146), Y155 (= Y161), K159 (= K165), P185 (= P191), G186 (= G192), W187 (= W193), V188 (= V194), T190 (= T196), L192 (= L198), V193 (≠ I199)
3w8dA Crystal structure of d-3-hydroxybutyrate dehydrogenase from alcaligenes faecalis complexed with NAD+ and an inhibitor methylmalonate
51% identity, 97% coverage: 8:266/266 of query aligns to 2:260/260 of 3w8dA
- active site: G15 (= G21), N114 (= N120), S142 (= S148), Y155 (= Y161), K159 (= K165), I200 (≠ V206)
- binding methylmalonic acid: Q94 (= Q100), S142 (= S148), H144 (= H150), K152 (= K158), Y155 (= Y161), W187 (= W193), Q196 (= Q202), W257 (= W263)
- binding nicotinamide-adenine-dinucleotide: G11 (= G17), T13 (= T19), S14 (= S20), G15 (= G21), I16 (= I22), F36 (≠ L42), A62 (= A68), D63 (= D69), L64 (= L70), N90 (= N96), A91 (= A97), G92 (= G98), L113 (≠ I119), S142 (= S148), Y155 (= Y161), K159 (= K165), P185 (= P191), G186 (= G192), W187 (= W193), V188 (= V194), T190 (= T196), L192 (= L198), V193 (≠ I199)
3vdrA Crystal structure of d-3-hydroxybutyrate dehydrogenase, prepared in the presence of the substrate d-3-hydroxybutyrate and NAD(+) (see paper)
51% identity, 97% coverage: 8:266/266 of query aligns to 2:260/260 of 3vdrA
- active site: G15 (= G21), N114 (= N120), S142 (= S148), Y155 (= Y161), K159 (= K165), I200 (≠ V206)
- binding (3R)-3-hydroxybutanoic acid: Q94 (= Q100), H144 (= H150), K152 (= K158), Y155 (= Y161), W187 (= W193), Q196 (= Q202), W257 (= W263)
- binding acetoacetic acid: Q94 (= Q100), H144 (= H150), K152 (= K158), Y155 (= Y161), W187 (= W193), Q196 (= Q202), W257 (= W263)
- binding nicotinamide-adenine-dinucleotide: G11 (= G17), T13 (= T19), I16 (= I22), F36 (≠ L42), D63 (= D69), L64 (= L70), N90 (= N96), A91 (= A97), G92 (= G98), L113 (≠ I119), K159 (= K165), G186 (= G192), V188 (= V194), T190 (= T196), L192 (= L198), V193 (≠ I199)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G11 (= G17), T13 (= T19), I16 (= I22), F36 (≠ L42), D63 (= D69), L64 (= L70), N90 (= N96), A91 (= A97), G92 (= G98), L113 (≠ I119), S142 (= S148), Y155 (= Y161), K159 (= K165), G186 (= G192), V188 (= V194), T190 (= T196), L192 (= L198), V193 (≠ I199)
3vdqA Crystal structure of alcaligenes faecalis d-3-hydroxybutyrate dehydrogenase in complex with NAD(+) and acetate (see paper)
51% identity, 97% coverage: 8:266/266 of query aligns to 2:260/260 of 3vdqA
- active site: G15 (= G21), N114 (= N120), S142 (= S148), Y155 (= Y161), K159 (= K165), I200 (≠ V206)
- binding acetate ion: Q94 (= Q100), H144 (= H150), K152 (= K158), W187 (= W193), L192 (= L198), Q196 (= Q202)
- binding nicotinamide-adenine-dinucleotide: G11 (= G17), S14 (= S20), I16 (= I22), F36 (≠ L42), D63 (= D69), L64 (= L70), N90 (= N96), A91 (= A97), G92 (= G98), L113 (≠ I119), I140 (= I146), S142 (= S148), Y155 (= Y161), K159 (= K165), P185 (= P191), G186 (= G192), W187 (= W193), V188 (= V194), T190 (= T196), L192 (= L198), V193 (≠ I199)
2ztlA Closed conformation of d-3-hydroxybutyrate dehydrogenase complexed with NAD+ and l-3-hydroxybutyrate (see paper)
51% identity, 97% coverage: 8:266/266 of query aligns to 2:260/260 of 2ztlA
- active site: G15 (= G21), N114 (= N120), S142 (= S148), Y155 (= Y161), K159 (= K165), L200 (≠ V206)
- binding (3s)-3-hydroxybutanoic acid: Q94 (= Q100), S142 (= S148), H144 (= H150), K152 (= K158), Y155 (= Y161), Q196 (= Q202)
- binding nicotinamide-adenine-dinucleotide: G11 (= G17), G15 (= G21), I16 (= I22), F36 (≠ L42), L64 (= L70), N90 (= N96), A91 (= A97), G92 (= G98), L113 (≠ I119), Y155 (= Y161), K159 (= K165), P185 (= P191), W187 (= W193), V188 (= V194), T190 (= T196), V193 (≠ I199)
1wmbA Crystal structure of NAD dependent d-3-hydroxybutylate dehydrogenase (see paper)
51% identity, 97% coverage: 8:266/266 of query aligns to 2:260/260 of 1wmbA
5yssB Crystal structure of aminocaproic acid cyclase in complex with NAD (+) (see paper)
53% identity, 98% coverage: 7:266/266 of query aligns to 1:255/255 of 5yssB
- binding nicotinamide-adenine-dinucleotide: G11 (= G17), T13 (= T19), S14 (= S20), G15 (= G21), I16 (= I22), G35 (= G41), F36 (≠ L42), L60 (= L70), N86 (= N96), G88 (= G98), I89 (= I99), A137 (= A147), Y151 (= Y161), K155 (= K165), P181 (= P191), G182 (= G192), V184 (= V194), T186 (= T196)
2q2qD Structure of d-3-hydroxybutyrate dehydrogenase from pseudomonas putida (see paper)
50% identity, 98% coverage: 7:266/266 of query aligns to 1:255/255 of 2q2qD
- active site: G15 (= G21), S138 (= S148), Y151 (= Y161), K155 (= K165), R196 (≠ V206)
- binding nicotinamide-adenine-dinucleotide: G11 (= G17), T13 (= T19), S14 (= S20), G15 (= G21), I16 (= I22), F36 (≠ L42), D59 (= D69), L60 (= L70), N86 (= N96), G88 (= G98), L109 (≠ I119), I136 (= I146), S138 (= S148), Y151 (= Y161), K155 (= K165), P181 (= P191), G182 (= G192), W183 (= W193), V184 (= V194), T186 (= T196), L188 (= L198), V189 (≠ I199)
1x1tA Crystal structure of d-3-hydroxybutyrate dehydrogenase from pseudomonas fragi complexed with NAD+ (see paper)
47% identity, 97% coverage: 8:266/266 of query aligns to 2:236/236 of 1x1tA
- active site: G15 (= G21), N114 (= N120), S142 (= S148), Y155 (= Y161), K159 (= K165)
- binding cacodylate ion: S142 (= S148), H144 (= H150), Y155 (= Y161), W187 (= W193), W233 (= W263)
- binding nicotinamide-adenine-dinucleotide: G11 (= G17), T13 (= T19), S14 (= S20), G15 (= G21), I16 (= I22), G35 (= G41), F36 (≠ L42), D63 (= D69), L64 (= L70), N90 (= N96), G92 (= G98), L113 (≠ I119), S142 (= S148), Y155 (= Y161), K159 (= K165), P185 (= P191), W187 (= W193), V188 (= V194), T190 (= T196)
6zzsD Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and 3-oxovalerate (see paper)
39% identity, 98% coverage: 6:266/266 of query aligns to 3:261/261 of 6zzsD
- active site: G18 (= G21), S143 (= S148), Y156 (= Y161)
- binding nicotinamide-adenine-dinucleotide: G14 (= G17), S17 (= S20), I19 (= I22), D38 (≠ G41), M39 (≠ L42), D64 (= D69), V65 (≠ L70), N91 (= N96), A92 (= A97), G93 (= G98), M141 (≠ I146), A142 (= A147), S143 (= S148), Y156 (= Y161), K160 (= K165), P186 (= P191), G187 (= G192), V189 (= V194), T191 (= T196), L193 (= L198)
- binding 3-oxidanylidenepentanoic acid: Q95 (= Q100), S143 (= S148), N145 (≠ H150), K153 (= K158), Y156 (= Y161), Q197 (= Q202)
6zzqA Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and acetoacetate (see paper)
39% identity, 98% coverage: 6:266/266 of query aligns to 2:260/260 of 6zzqA
- active site: G17 (= G21), S142 (= S148), Y155 (= Y161)
- binding acetoacetic acid: Q94 (= Q100), S142 (= S148), K152 (= K158), Y155 (= Y161), Q196 (= Q202)
- binding nicotinamide-adenine-dinucleotide: G13 (= G17), S16 (= S20), G17 (= G21), I18 (= I22), D37 (≠ G41), M38 (≠ L42), D63 (= D69), V64 (≠ L70), N90 (= N96), A91 (= A97), G92 (= G98), M140 (≠ I146), A141 (= A147), S142 (= S148), Y155 (= Y161), K159 (= K165), Y187 (≠ W193), V188 (= V194), T190 (= T196)
3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
36% identity, 97% coverage: 7:263/266 of query aligns to 5:244/247 of 3op4A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G17), S17 (≠ T19), R18 (≠ S20), I20 (= I22), T40 (≠ P44), N62 (≠ D69), V63 (≠ L70), N89 (= N96), A90 (= A97), I92 (= I99), V139 (≠ I146), S141 (= S148), Y154 (= Y161), K158 (= K165), P184 (= P191), G185 (= G192), I187 (≠ V194), T189 (= T196), M191 (≠ L198)
4i08A Crystal structure of beta-ketoacyl-acyl carrier protein reductase (fabg) from vibrio cholerae in complex with NADPH (see paper)
35% identity, 97% coverage: 7:263/266 of query aligns to 5:240/243 of 4i08A
- active site: G19 (= G21), N113 (= N120), S141 (= S148), Q151 (≠ K158), Y154 (= Y161), K158 (= K165)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G17), S17 (≠ T19), R18 (≠ S20), I20 (= I22), T40 (≠ P44), N62 (≠ D69), V63 (≠ L70), N89 (= N96), A90 (= A97), G140 (≠ A147), S141 (= S148), Y154 (= Y161), K158 (= K165), P184 (= P191), G185 (= G192), T189 (= T196)
6ixmC Crystal structure of the ketone reductase chkred20 from the genome of chryseobacterium sp. Ca49 complexed with NAD (see paper)
33% identity, 97% coverage: 8:265/266 of query aligns to 3:247/248 of 6ixmC
- active site: G16 (= G21), S142 (= S148), Y155 (= Y161), K159 (= K165)
- binding nicotinamide-adenine-dinucleotide: G12 (= G17), S15 (= S20), G16 (= G21), I17 (= I22), D36 (≠ G41), I37 (≠ L42), A61 (= A68), D62 (= D69), T63 (≠ L70), N89 (= N96), A90 (= A97), M140 (≠ I146), S142 (= S148), Y155 (= Y161), K159 (= K165), P185 (= P191), A186 (≠ G192), Y187 (≠ W193), I188 (≠ V194), L192 (= L198)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
34% identity, 97% coverage: 7:263/266 of query aligns to 2:245/247 of 4jroC
- active site: G16 (= G21), S142 (= S148), Q152 (≠ K158), Y155 (= Y161), K159 (= K165)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G17), S14 (≠ T19), R15 (≠ S20), G16 (= G21), I17 (= I22), N35 (vs. gap), Y36 (≠ H40), N37 (≠ G41), G38 (≠ L42), S39 (≠ L43), N63 (≠ D69), V64 (≠ L70), N90 (= N96), A91 (= A97), I93 (= I99), I113 (= I119), S142 (= S148), Y155 (= Y161), K159 (= K165), P185 (= P191), I188 (≠ V194), T190 (= T196)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
32% identity, 96% coverage: 8:262/266 of query aligns to 5:241/244 of 4nbuB
- active site: G18 (= G21), N111 (= N120), S139 (= S148), Q149 (≠ K158), Y152 (= Y161), K156 (= K165)
- binding acetoacetyl-coenzyme a: D93 (≠ T102), K98 (≠ S107), S139 (= S148), N146 (≠ S155), V147 (= V156), Q149 (≠ K158), Y152 (= Y161), F184 (≠ W193), M189 (≠ L198), K200 (≠ L221)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G17), N17 (≠ S20), G18 (= G21), I19 (= I22), D38 (≠ G41), F39 (≠ L42), V59 (≠ A68), D60 (= D69), V61 (≠ L70), N87 (= N96), A88 (= A97), G89 (= G98), I90 (= I99), T137 (≠ I146), S139 (= S148), Y152 (= Y161), K156 (= K165), P182 (= P191), F184 (≠ W193), T185 (≠ V194), T187 (= T196), M189 (≠ L198)
6t77A Crystal structure of klebsiella pneumoniae fabg(NADPH-dependent) NADP- complex at 1.75 a resolution (see paper)
35% identity, 97% coverage: 7:263/266 of query aligns to 2:241/244 of 6t77A
- active site: G16 (= G21), S138 (= S148), Y151 (= Y161)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G17), S14 (≠ T19), R15 (≠ S20), T37 (≠ P44), L58 (≠ F65), N59 (≠ S66), V60 (≠ N67), A87 (= A97), G88 (= G98), I89 (= I99)
P16544 Putative ketoacyl reductase; EC 1.3.1.- from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see 2 papers)
33% identity, 95% coverage: 9:262/266 of query aligns to 5:257/261 of P16544
- 11:39 (vs. 15:45, 35% identical) binding
- D63 (= D69) binding
- K161 (= K165) binding
2rh4B Actinorhodin ketoreductase, actkr, with NADPH and inhibitor emodin (see paper)
33% identity, 95% coverage: 9:262/266 of query aligns to 12:264/268 of 2rh4B
- active site: G24 (= G21), N121 (= N120), S151 (= S148), Y164 (= Y161), K168 (= K165), Y209 (≠ V206)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G20 (= G17), T22 (= T19), S23 (= S20), I25 (= I22), A44 (≠ L43), R45 (≠ P44), G46 (≠ E45), C69 (≠ A68), D70 (= D69), V71 (≠ L70), N97 (= N96), S151 (= S148), Y164 (= Y161), K168 (= K165), G195 (= G192), V197 (= V194), T199 (= T196), M201 (≠ L198)
1xr3A Actinorhodin polyketide ketoreductase with NADP and the inhibitor isoniazid bound (see paper)
33% identity, 95% coverage: 11:262/266 of query aligns to 2:252/256 of 1xr3A
- active site: G12 (= G21), N109 (= N120), S139 (= S148), Y152 (= Y161), K156 (= K165), Y197 (≠ V206)
- binding 4-(diazenylcarbonyl)pyridine: T140 (≠ V149), G141 (≠ H150), V146 (≠ S155)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G17), T10 (= T19), S11 (= S20), G12 (= G21), I13 (= I22), A32 (≠ L43), R33 (≠ P44), G34 (≠ E45), C57 (≠ A68), D58 (= D69), V59 (≠ L70), N85 (= N96), A86 (= A97), G87 (= G98), S139 (= S148), Y152 (= Y161), K156 (= K165), G183 (= G192), V185 (= V194), T187 (= T196), P188 (= P197)
Query Sequence
>6936328 FitnessBrowser__SB2B:6936328
MSGGQRSLEGKVGLITGSTSGIGLATAQVLAEQGCNLILHGLLPEDEGQSMAADFAAQYR
IRTFFSNADLRQPESIHRFMAEGTKALGSIDILINNAGIQHTDSVASFPIEKWNDIIAIN
LSSAFHTMQQAVPAMAQKRWGRIINIASVHGLVASVNKAAYCAAKHGIVGLTKVVAIECA
EQGITVNAICPGWVDTPLINKQIEAVANTKGLDYHEARYQLVTAKQPLPEMLDPRQIGEF
ALFLCGDAARGITGASLAMDGGWTAQ
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory