SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 6936480 Sama_0668 iron(III) ABC transporter, ATP-binding protein (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
37% identity, 89% coverage: 4:308/341 of query aligns to 18:333/378 of P69874

query
sites
P69874

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C26 (≠ D12) mutation to A: Lower ATPase activity and transport efficiency.
F27 (≠ Y13) mutation to L: Lower ATPase activity and transport efficiency.
F45 (≠ I31) mutation to L: Lower ATPase activity and transport efficiency.
C54 (= C40) mutation to T: Loss of ATPase activity and transport.
L60 (= L46) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ I62) mutation to P: Lower ATPase activity and transport efficiency.
V135 (= V123) mutation to M: Loss of ATPase activity and transport.
D172 (= D160) mutation to N: Loss of ATPase activity and transport.
C276 (≠ L260) mutation to A: Lower ATPase activity and transport efficiency.
E297 (= E278) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
36% identity, 92% coverage: 1:315/341 of query aligns to 4:318/353 of 1vciA

query
sites
1vciA

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binding adenosine-5'-triphosphate: F16 (≠ Y13), F19 (≠ E16), P40 (= P37), S41 (= S38), G42 (= G39), C43 (= C40), G44 (= G41), K45 (= K42), T46 (= T43), T47 (= T44)

1g291 Malk (see paper)
40% identity, 77% coverage: 19:280/341 of query aligns to 19:294/372 of 1g291

query
sites
1g291

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binding magnesium ion: D69 (vs. gap), E71 (≠ A69), K72 (≠ D70), K79 (≠ E77), D80 (≠ K78), Y228 (= Y224), D229 (≠ Q225), E292 (= E278), D293 (≠ Q279)
binding pyrophosphate 2-: P37 (= P37), S38 (= S38), G39 (= G39), C40 (= C40), G41 (= G41), K42 (= K42), T43 (= T43), T44 (= T44)

Sites not aligning to the query:

binding magnesium ion: 341, 342, 359

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
42% identity, 66% coverage: 19:243/341 of query aligns to 22:252/375 of 2d62A

query
sites
2d62A

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binding pyrophosphate 2-: P40 (= P37), S41 (= S38), G42 (= G39), C43 (= C40), G44 (= G41), K45 (= K42), T46 (= T43), T47 (= T44)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
36% identity, 88% coverage: 1:300/341 of query aligns to 1:312/371 of P68187

query
sites
P68187

M

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L

L

R

R

T

M

I

I

A

A

G

G

L

L

Q

E

D

T

I

I

S

T

A

S

G

G

S

D

I

L

A

F

I

I

N

G

G

E

K

K

T

R

V

M

S

N

A

D

D

-

G

-

C

-

F

-

V

T

A

P

P

P

E

A

K

E

R

R

S

G

I

V

G

G

M

M

I

V

F

F

Q

Q

D

S

Y

Y

A
|
A

L

L

F

Y

P

P

H

H

L

L

T

S

V

V

A

A

D

E

N

N

I

M

L

S

F

F

G

G

V

L

R

K

-

L

-

A

Q

G

L

A

D
x
K

K

K

Q

E

S

V

R

I

S

N

V

Q

R

R

L
x
V

E

N

E

Q

M
x
V

L

A

S
x
E

L

V

V

L

K

Q

L

L

E
x
A

G

H

L

L

G

L

K

D

R

R

Y

K

P

P

H

K

E

A

L

L

S

S

G

G

G
|
G

Q

Q

Q

R

Q

Q

R

R

V

V

S

A

I

I

A

G

R

R

A

T

L

L

A

V

Y

A

E

E

P

P

D

S

L

V

L

F

L

L

D
|
D

E

E

P

P

F

L

S

S

N

N

I

L

D

D

A

A

Q

A

V

L

R

R

R

V

A

Q

L

M

M

R

L

I

E

E

I

I

R

S

A

R

I

L

L

H

K

K

A

R

R

L

N

G

V

R

S

T

A

M

V

I

F

Y

V

V

T

T

H

H

S

D

K

Q

D

V

E

E

A

A

F

M

A

T

F

L

A

A

D

D

T

K

L

I

A

V

L

V

F

L

E

D

A

A

G

G

R

R

I

V

V

A

Q

Q

H

V

G

G

I

K

P

P

E

L

T

E

L

L

Y

Y

Q

H

S

Y

P

P

N

A

T

D

P
x
R

Y

F

V

V

A

A

D

G

F

F

L

I

G

G

A

S

S

P

-

K

-

M

N

N

Y
x
F

L

L

D

P

V

V

R

K

L

V

E

T

A

A

G

T

Q

A

L

I

I

D

S

Q

T

V

L

Q

G

V

A

E

F

L

P

P

L

M

P

P

Q

N

D

R

F

Q

K

Q

A

V

A

-

S

-

E

-

T

-

G

-

R

-

W
|
W

L

L

-

P

-

V

-

E

-

S

-

R

-

D

-

V

-

Q

-

V

-
x
G

-

A

-

N

-

M

-
x
S

-

L

-
x
G

L

I

R

R

P

P

E

E

Q

H

L

L

I

P

E

S

A

D

R

I

A

A

D

D

G

V

-

I

-

L

A

E

G
|
G

E

E

I

V

L

Q

E

V

R

V

R
x
E

F

Q

L

L

G

G

N

N

A85 (= A89) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ D108) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (≠ L116) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ M119) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ S121) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ E126) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G139) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D160) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (≠ P230) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
F241 (≠ Y241) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
W267 (= W273) mutation to G: Normal maltose transport but constitutive mal gene expression.
G278 (vs. gap) mutation to P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
S282 (vs. gap) mutation to L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G284 (vs. gap) mutation to S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G302 (= G290) mutation to D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
E308 (≠ R296) mutation to Q: Maltose transport is affected but retains ability to interact with MalT.

Sites not aligning to the query:

322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

2awoA Crystal structure of the adp-mg-bound e. Coli malk (crystallized with adp-mg) (see paper)
36% identity, 88% coverage: 2:300/341 of query aligns to 1:311/372 of 2awoA

query
sites
2awoA

S

A

T

S

L

V

S

Q

I

L

Q

Q

G

N

L

V

H

T

S

K

D

A

Y
x
W

R

G

G

E

E

V

Q

V

V
|
V

L

S

R

K

G

D

L

I

N

N

L

L

T

D

L

I

T

H

Q

E

G

G

E

E

I

F

T

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

T

M

I

I

A

A

G

G

L

L

Q

E

D

T

I

I

S

T

A

S

G

G

S

D

I

L

A

F

I

I

N

G

G

E

K

K

T

R

V

M

S

N

A

D

D

-

G

-

C

-

F

-

V

T

A

P

P

P

E

A

K

E

R

R

S

G

I

V

G

G

M

M

I

V

F

F

Q

Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

L

L

T

S

V

V

A

A

D

E

N

N

I

M

L

S

F

F

G

G

V

L

R

K

-

L

-

A

Q

G

L

A

D

K

K

K

Q

E

S

V

R

I

S

N

V

Q

R

R

L

V

E

N

E

Q

M

V

L

A

S

E

L

V

V

L

K

Q

L

L

E

A

G

H

L

L

G

L

K

D

R

R

Y

K

P

P

H

K

E

A

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

S

A

I

I

A

G

R

R

A

T

L

L

A

V

Y

A

E

E

P

P

D

S

L

V

L

F

L

L

D
|
D

E

E

P

P

F

L

S

S

N

N

I

L

D

D

A

A

Q

A

V

L

R

R

R

V

A

Q

L

M

M

R

L

I

E

E

I

I

R

S

A

R

I

L

L

H

K

K

A

R

R

L

N

G

V

R

S

T

A

M

V

I

F

Y

V

V

T

T

H

H

S

D

K

Q

D

V

E

E

A

A

F

M

A

T

F

L

A

A

D

D

T

K

L

I

A

V

L

V

F

L

E

D

A

A

G

G

R

R

I

V

V

A

Q

Q

H

V

G

G

I

K

P

P

E

L

T

E

L

L

Y

Y

Q

H

S

Y

P

P

N

A

T

D

P

R

Y

F

V

V

A

A

D

G

F

F

L

I

G

G

A

S

S

P

-

K

-

M

N

N

Y

F

L

L

D

P

V

V

R

K

L

V

E

T

A

A

G

T

Q

A

L

I

I

D

S

Q

T

V

L

Q

G

V

A

E

F

L

P

P

L

M

P

P

Q

N

D

R

F

Q

K

Q

A

V

A

-

S

-

E

-

T

-

G

-

R

-

W

W

L

L

-

P

-

V

-

E

-

S

-

R

-

D

-

V

-

Q

-

V

-

G

-

A

-

N

-

M

-

S

-

L

-

G

L

I

R

R

P

P

E

E

Q

H

L

L

I

P

E

S

A

D

R

I

A

A

D

D

G

V

-

I

-

L

A

E

G

G

E

E

I

V

L

Q

E

V

R

V

R

E

F

Q

L

L

G

G

N

N

binding adenosine-5'-diphosphate: W12 (≠ Y13), V17 (= V18), S37 (= S38), G38 (= G39), C39 (= C40), G40 (= G41), K41 (= K42), S42 (≠ T43), T43 (= T44)
binding magnesium ion: S42 (≠ T43), D157 (= D160)

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
36% identity, 88% coverage: 2:300/341 of query aligns to 1:311/371 of 3puyA

query
sites
3puyA

S

A

T

S

L

V

S

Q

I

L

Q

Q

G

N

L

V

H

T

S

K

D

A

Y
x
W

R

G

G

E

E

V

Q

V

V
|
V

L

S

R

K

G

D

L

I

N

N

L

L

T

D

L

I

T

H

Q

E

G

G

E

E

I

F

T

V

A

V

L

F

L

V

G

G

P
|
P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

T

M

I

I

A

A

G

G

L

L

Q

E

D

T

I

I

S

T

A

S

G

G

S

D

I

L

A

F

I

I

N

G

G

E

K

K

T

R

V

M

S

N

A

D

D

-

G

-

C

-

F

-

V

T

A

P

P

P

E

A

K

E

R

R

S

G

I

V

G

G

M

M

I

V

F

F

Q
|
Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

L

L

T

S

V

V

A

A

D

E

N

N

I

M

L

S

F

F

G

G

V

L

R

K

-

L

-

A

Q

G

L

A

D

K

K

K

Q

E

S

V

R

I

S

N

V

Q

R

R

L

V

E

N

E

Q

M

V

L

A

S

E

L

V

V

L

K

Q

L

L

E

A

G

H

L

L

G

L

K

D

R
|
R

Y

K

P

P

H

K

E
x
A

L

L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

S

A

I

I

A

G

R

R

A

T

L

L

A

V

Y

A

E

E

P

P

D

S

L

V

L

F

L

L

D
|
D

E
|
E

P

P

F

L

S

S

N

N

I

L

D

D

A

A

Q

A

V

L

R

R

R

V

A

Q

L

M

M

R

L

I

E

E

I

I

R

S

A

R

I

L

L

H

K

K

A

R

R

L

N

G

V

R

S

T

A

M

V

I

F

Y

V

V

T

T

H
|
H

S

D

K

Q

D

V

E

E

A

A

F

M

A

T

F

L

A

A

D

D

T

K

L

I

A

V

L

V

F

L

E

D

A

A

G

G

R

R

I

V

V

A

Q

Q

H

V

G

G

I

K

P

P

E

L

T

E

L

L

Y

Y

Q

H

S

Y

P

P

N

A

T

D

P

R

Y

F

V

V

A

A

D

G

F

F

L

I

G

G

A

S

S

P

-

K

-

M

N

N

Y

F

L

L

D

P

V

V

R

K

L

V

E

T

A

A

G

T

Q

A

L

I

I

D

S

Q

T

V

L

Q

G

V

A

E

F

L

P

P

L

M

P

P

Q

N

D

R

F

Q

K

Q

A

V

A

-

S

-

E

-

T

-

G

-

R

-

W

W

L

L

-

P

-

V

-

E

-

S

-

R

-

D

-

V

-

Q

-

V

-

G

-

A

-

N

-

M

-

S

-

L

-

G

L

I

R

R

P

P

E

E

Q

H

L

L

I

P

E

S

A

D

R

I

A

A

D

D

G

V

-

I

-

L

A

E

G

G

E

E

I

V

L

Q

E

V

R

V

R

E

F

Q

L

L

G

G

N

N

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y13), V17 (= V18), P36 (= P37), S37 (= S38), G38 (= G39), C39 (= C40), G40 (= G41), K41 (= K42), S42 (≠ T43), T43 (= T44), Q81 (= Q86), R128 (= R131), A132 (≠ E135), S134 (= S137), G135 (= G138), G136 (= G139), Q137 (= Q140), E158 (= E161), H191 (= H194)
binding magnesium ion: S42 (≠ T43), Q81 (= Q86), D157 (= D160)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
36% identity, 88% coverage: 2:300/341 of query aligns to 1:311/371 of 3puxA

query
sites
3puxA

S

A

T

S

L

V

S

Q

I

L

Q

Q

G

N

L

V

H

T

S

K

D

A

Y
x
W

R

G

G

E

E

V

Q

V

V
|
V

L

S

R

K

G

D

L

I

N

N

L

L

T

D

L

I

T

H

Q

E

G

G

E

E

I

F

T

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

T

M

I

I

A

A

G

G

L

L

Q

E

D

T

I

I

S

T

A

S

G

G

S

D

I

L

A

F

I

I

N

G

G

E

K

K

T

R

V

M

S

N

A

D

D

-

G

-

C

-

F

-

V

T

A

P

P

P

E

A

K

E

R

R

S

G

I

V

G

G

M

M

I

V

F

F

Q
|
Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

L

L

T

S

V

V

A

A

D

E

N

N

I

M

L

S

F

F

G

G

V

L

R

K

-

L

-

A

Q

G

L

A

D

K

K

K

Q

E

S

V

R

I

S

N

V

Q

R

R

L

V

E

N

E

Q

M

V

L

A

S

E

L

V

V

L

K

Q

L

L

E

A

G

H

L

L

G

L

K

D

R
|
R

Y

K

P

P

H

K

E
x
A

L
|
L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

S

A

I

I

A

G

R

R

A

T

L

L

A

V

Y

A

E

E

P

P

D

S

L

V

L

F

L

L

D
|
D

E
|
E

P

P

F

L

S

S

N

N

I

L

D

D

A

A

Q

A

V

L

R

R

R

V

A

Q

L

M

M

R

L

I

E

E

I

I

R

S

A

R

I

L

L

H

K

K

A

R

R

L

N

G

V

R

S

T

A

M

V

I

F

Y

V

V

T

T

H
|
H

S

D

K

Q

D

V

E

E

A

A

F

M

A

T

F

L

A

A

D

D

T

K

L

I

A

V

L

V

F

L

E

D

A

A

G

G

R

R

I

V

V

A

Q

Q

H

V

G

G

I

K

P

P

E

L

T

E

L

L

Y

Y

Q

H

S

Y

P

P

N

A

T

D

P

R

Y

F

V

V

A

A

D

G

F

F

L

I

G

G

A

S

S

P

-

K

-

M

N

N

Y

F

L

L

D

P

V

V

R

K

L

V

E

T

A

A

G

T

Q

A

L

I

I

D

S

Q

T

V

L

Q

G

V

A

E

F

L

P

P

L

M

P

P

Q

N

D

R

F

Q

K

Q

A

V

A

-

S

-

E

-

T

-

G

-

R

-

W

W

L

L

-

P

-

V

-

E

-

S

-

R

-

D

-

V

-

Q

-

V

-

G

-

A

-

N

-

M

-

S

-

L

-

G

L

I

R

R

P

P

E

E

Q

H

L

L

I

P

E

S

A

D

R

I

A

A

D

D

G

V

-

I

-

L

A

E

G

G

E

E

I

V

L

Q

E

V

R

V

R

E

F

Q

L

L

G

G

N

N

binding adenosine-5'-diphosphate: W12 (≠ Y13), V17 (= V18), S37 (= S38), G38 (= G39), C39 (= C40), G40 (= G41), K41 (= K42), S42 (≠ T43), T43 (= T44), R128 (= R131), A132 (≠ E135), L133 (= L136), S134 (= S137), Q137 (= Q140)
binding beryllium trifluoride ion: S37 (= S38), G38 (= G39), K41 (= K42), Q81 (= Q86), S134 (= S137), G135 (= G138), G136 (= G139), E158 (= E161), H191 (= H194)
binding magnesium ion: S42 (≠ T43), Q81 (= Q86), D157 (= D160)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
36% identity, 88% coverage: 2:300/341 of query aligns to 1:311/371 of 3puwA

query
sites
3puwA

S

A

T

S

L

V

S

Q

I

L

Q

Q

G

N

L

V

H

T

S

K

D

A

Y
x
W

R

G

G

E

E

V

Q

V

V
|
V

L

S

R

K

G

D

L

I

N

N

L

L

T

D

L

I

T

H

Q

E

G

G

E

E

I

F

T

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

T

M

I

I

A

A

G

G

L

L

Q

E

D

T

I

I

S

T

A

S

G

G

S

D

I

L

A

F

I

I

N

G

G

E

K

K

T

R

V

M

S

N

A

D

D

-

G

-

C

-

F

-

V

T

A

P

P

P

E

A

K

E

R

R

S

G

I

V

G

G

M

M

I

V

F

F

Q
|
Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

L

L

T

S

V

V

A

A

D

E

N

N

I

M

L

S

F

F

G

G

V

L

R

K

-

L

-

A

Q

G

L

A

D

K

K

K

Q

E

S

V

R

I

S

N

V

Q

R

R

L

V

E

N

E

Q

M

V

L

A

S

E

L

V

V

L

K

Q

L

L

E

A

G

H

L

L

G

L

K

D

R
|
R

Y

K

P

P

H

K

E
x
A

L

L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

S

A

I

I

A

G

R

R

A

T

L

L

A

V

Y

A

E

E

P

P

D

S

L

V

L

F

L

L

D

D

E
|
E

P

P

F

L

S

S

N
|
N

I

L

D

D

A

A

Q

A

V

L

R

R

R

V

A

Q

L

M

M

R

L

I

E

E

I

I

R

S

A

R

I

L

L

H

K

K

A

R

R

L

N

G

V

R

S

T

A

M

V

I

F

Y

V

V

T

T

H
|
H

S

D

K

Q

D

V

E

E

A

A

F

M

A

T

F

L

A

A

D

D

T

K

L

I

A

V

L

V

F

L

E

D

A

A

G

G

R

R

I

V

V

A

Q

Q

H

V

G

G

I

K

P

P

E

L

T

E

L

L

Y

Y

Q

H

S

Y

P

P

N

A

T

D

P

R

Y

F

V

V

A

A

D

G

F

F

L

I

G

G

A

S

S

P

-

K

-

M

N

N

Y

F

L

L

D

P

V

V

R

K

L

V

E

T

A

A

G

T

Q

A

L

I

I

D

S

Q

T

V

L

Q

G

V

A

E

F

L

P

P

L

M

P

P

Q

N

D

R

F

Q

K

Q

A

V

A

-

S

-

E

-

T

-

G

-

R

-

W

W

L

L

-

P

-

V

-

E

-

S

-

R

-

D

-

V

-

Q

-

V

-

G

-

A

-

N

-

M

-

S

-

L

-

G

L

I

R

R

P

P

E

E

Q

H

L

L

I

P

E

S

A

D

R

I

A

A

D

D

G

V

-

I

-

L

A

E

G

G

E

E

I

V

L

Q

E

V

R

V

R

E

F

Q

L

L

G

G

N

N

binding adenosine-5'-diphosphate: W12 (≠ Y13), V17 (= V18), S37 (= S38), G38 (= G39), C39 (= C40), G40 (= G41), K41 (= K42), S42 (≠ T43), T43 (= T44), R128 (= R131), A132 (≠ E135), S134 (= S137), Q137 (= Q140)
binding tetrafluoroaluminate ion: S37 (= S38), G38 (= G39), K41 (= K42), Q81 (= Q86), S134 (= S137), G135 (= G138), G136 (= G139), E158 (= E161), N162 (= N165), H191 (= H194)
binding magnesium ion: S42 (≠ T43), Q81 (= Q86)

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
36% identity, 88% coverage: 2:300/341 of query aligns to 1:311/374 of 2awnB

query
sites
2awnB

S

A

T

S

L

V

S

Q

I

L

Q

Q

G

N

L

V

H

T

S

K

D

A

Y
x
W

R

G

G

E

E

V

Q

V

V
|
V

L

S

R

K

G

D

L

I

N

N

L

L

T

D

L

I

T

H

Q

E

G

G

E

E

I

F

T

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

T

M

I

I

A

A

G

G

L

L

Q

E

D

T

I

I

S

T

A

S

G

G

S

D

I

L

A

F

I

I

N

G

G

E

K

K

T

R

V

M

S

N

A

D

D

-

G

-

C

-

F

-

V

T

A

P

P

P

E

A

K

E

R

R

S

G

I

V

G

G

M

M

I

V

F

F

Q

Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

L

L

T

S

V

V

A

A

D

E

N

N

I

M

L

S

F

F

G

G

V

L

R

K

-

L

-

A

Q

G

L

A

D

K

K

K

Q

E

S

V

R

I

S

N

V

Q

R

R

L

V

E

N

E

Q

M

V

L

A

S

E

L

V

V

L

K

Q

L

L

E

A

G

H

L

L

G

L

K

D

R

R

Y

K

P

P

H

K

E

A

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

S

A

I

I

A

G

R

R

A

T

L

L

A

V

Y

A

E

E

P

P

D

S

L

V

L

F

L

L

D

D

E

E

P

P

F

L

S

S

N

N

I

L

D

D

A

A

Q

A

V

L

R

R

R

V

A

Q

L

M

M

R

L

I

E

E

I

I

R

S

A

R

I

L

L

H

K

K

A

R

R

L

N

G

V

R

S

T

A

M

V

I

F

Y

V

V

T

T

H

H

S

D

K

Q

D

V

E

E

A

A

F

M

A

T

F

L

A

A

D

D

T

K

L

I

A

V

L

V

F

L

E

D

A

A

G

G

R

R

I

V

V

A

Q

Q

H

V

G

G

I

K

P

P

E

L

T

E

L

L

Y

Y

Q

H

S

Y

P

P

N

A

T

D

P

R

Y

F

V

V

A

A

D

G

F

F

L

I

G

G

A

S

S

P

-

K

-

M

N

N

Y

F

L

L

D

P

V

V

R

K

L

V

E

T

A

A

G

T

Q

A

L

I

I

D

S

Q

T

V

L

Q

G

V

A

E

F

L

P

P

L

M

P

P

Q

N

D

R

F

Q

K

Q

A

V

A

-

S

-

E

-

T

-

G

-

R

-

W

W

L

L

-

P

-

V

-

E

-

S

-

R

-

D

-

V

-

Q

-

V

-

G

-

A

-

N

-

M

-

S

-

L

-

G

L

I

R

R

P

P

E

E

Q

H

L

L

I

P

E

S

A

D

R

I

A

A

D

D

G

V

-

I

-

L

A

E

G

G

E

E

I

V

L

Q

E

V

R

V

R

E

F

Q

L

L

G

G

N

N

binding adenosine-5'-diphosphate: W12 (≠ Y13), V17 (= V18), S37 (= S38), G38 (= G39), C39 (= C40), G40 (= G41), K41 (= K42), S42 (≠ T43), T43 (= T44)

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
37% identity, 84% coverage: 15:300/341 of query aligns to 11:309/367 of 1q12A

query
sites
1q12A

G

G

E

E

Q

V

V

V

L
x
V

-

S

R

K

G

D

L

I

N

N

L

L

T

D

L

I

T

H

Q

E

G

G

E

E

I

F

T

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

T

M

I

I

A

A

G

G

L

L

Q

E

D

T

I

I

S

T

A

S

G

G

S

D

I

L

A

F

I

I

N

G

G

E

K

K

T

R

V

M

S

N

A

D

D

-

G

-

C

-

F

-

V

T

A

P

P

P

E

A

K

E

R

R

S

G

I

V

G

G

M

M

I

V

F

F

Q

Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

L

L

T

S

V

V

A

A

D

E

N

N

I

M

L

S

F

F

G

G

V

L

R

K

-

L

-

A

Q

G

L

A

D

K

K

K

Q

E

S

V

R

I

S

N

V

Q

R

R

L

V

E

N

E

Q

M

V

L

A

S

E

L

V

V

L

K

Q

L

L

E

A

G

H

L

L

G

L

K

D

R
|
R

Y

K

P

P

H
x
K

E
x
A

L
|
L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

S

A

I

I

A

G

R

R

A

T

L

L

A

V

Y

A

E

E

P

P

D

S

L

V

L

F

L

L

D

D

E

E

P

P

F

L

S

S

N
|
N

I

L

D

D

A

A

Q

A

V

L

R

R

R

V

A

Q

L

M

M

R

L

I

E

E

I

I

R

S

A

R

I

L

L

H

K

K

A

R

R

L

N

G

V

R

S

T

A

M

V

I

F

Y

V

V

T

T

H
|
H

S

D

K

Q

D

V

E

E

A

A

F

M

A

T

F

L

A

A

D

D

T

K

L

I

A

V

L

V

F

L

E

D

A

A

G

G

R

R

I

V

V

A

Q

Q

H

V

G

G

I

K

P

P

E

L

T

E

L

L

Y

Y

Q

H

S

Y

P

P

N

A

T

D

P

R

Y

F

V

V

A

A

D

G

F

F

L

I

G

G

A

S

S

P

-

K

-

M

N

N

Y

F

L

L

D

P

V

V

R

K

L

V

E

T

A

A

G

T

Q

A

L

I

I

D

S

Q

T

V

L

Q

G

V

A

E

F

L

P

P

L

M

P

P

Q

N

D

R

F

Q

K

Q

A

V

A

-

S

-

E

-

T

-

G

-

R

-

W

W

L

L

-

P

-

V

-

E

-

S

-

R

-

D

-

V

-

Q

-

V

-

G

-

A

-

N

-

M

-

S

-

L

-

G

L

I

R

R

P

P

E

E

Q

H

L

L

I

P

E

S

A

D

R

I

A

A

D

D

G

V

-

I

-

L

A

E

G

G

E

E

I

V

L

Q

E

V

R

V

R

E

F

Q

L

L

G

G

N

N

binding adenosine-5'-triphosphate: V15 (≠ L19), S35 (= S38), G36 (= G39), C37 (= C40), G38 (= G41), K39 (= K42), S40 (≠ T43), T41 (= T44), R126 (= R131), K129 (≠ H134), A130 (≠ E135), L131 (= L136), S132 (= S137), G133 (= G138), G134 (= G139), Q135 (= Q140), N160 (= N165), H189 (= H194)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 10

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
37% identity, 84% coverage: 1:287/341 of query aligns to 1:295/369 of P19566

query
sites
P19566

M

M

S

A

T

S

L

V

S

Q

I

L

Q

R

G

N

L

V

H

T

S

K

D

A

Y

W

R

G

G

D

E

V

Q

V

V

V

L

S

R

K

G

D

L

I

N

N

L

L

T

D

L

I

T

H

Q

D

G

G

E

E

I

F

T

V

A

V

L

F

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

S

T

T

L

L

R

R

T

M

I

I

A

A

G

G

L

L

Q

E

D

T

I

I

S

T

A

S

G

G

S

D

I

L

A

F

I

I

N

-

G

G

K

E

T

T

V

R

S

M

A

N

D

D

G

-

C

-

F

-

V

I

A

P

P

P

E

A

K

E

R

R

S

G

I

V

G

G

M

M

I

V

F

F

Q

Q

D

S

Y

Y

A

A

L
|
L

F

Y

P

P

H

H

L

L

T

S

V

V

A

A

D

E

N

N

I

M

L

S

F

F

G

G

V

L

R

K

-

L

-

A

Q

G

L

A

D

K

K

K

Q

E

S

V

R

M

S

N

V

Q

R

R

L

V

E

N

E

Q

M

V

L

A

S

E

L

V

V

L

K

Q

L

L

E

A

G

H

L

L

G

L

K

E

R

R

Y

K

P

P

H

K

E

A

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

S

A

I

I

A

G

R

R

A

T

L

L

A

V

Y

A

E

E

P

P

D

R

L

V

L

F

L

L

D

D

E

E

P
|
P

F

L

S

S

N

N

I

L

D
|
D

A

A

Q

A

V

L

R

R

R

V

A

Q

L

M

M

R

L

I

E

E

I

I

R

S

A

R

I

L

L

H

K

K

A

R

R

L

N

G

V

R

S

T

A

M

V

I

F

Y

V

V

T

T

H

H

S

D

K

Q

D

V

E

E

A

A

F

M

A

T

F

L

A

A

D

D

T

K

L

I

A

V

L

V

F

L

E

D

A

A

G

G

R

R

I

V

V

A

Q

Q

H

V

G

G

I

K

P

P

E

L

T

E

L

L

Y

Y

Q

H

S

Y

P

P

N

A

T

D

P

R

Y

F

V

V

A

A

D

G

F

F

L

I

G

G

A

S

S

P

-

K

-

M

N

N

Y

F

L

L

D

P

V

V

R

K

L

V

E

T

A

A

G

T

Q

A

L

I

I

E

S

Q

T

V

L

Q

G

V

A

E

F

L

P

P

-

N

-

R

-

Q

-

I

-

W

L

L

P

P

Q

V

D

E

F

S

K

R

A

G

A

V

S

Q

E

V

T

G

G

A

R

N

W

M

L

S

L

L

-

G

-

I

R

R

P

P

E

E

Q

H

L

L

I

P

E

S

A

D

R

I

A

A

D

D

L86 (= L90) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P162) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D167) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

3fvqB Crystal structure of the nucleotide binding domain fbpc complexed with atp (see paper)
41% identity, 85% coverage: 2:292/341 of query aligns to 2:294/350 of 3fvqB

query
sites
3fvqB

S

A

T

A

L

L

S

H

I

I

Q

G

G

H

L

L

H

S

S

K

D

S

Y
x
F

R
x
Q

G

N

E
x
T

Q

P

V
|
V

L

L

R

N

G

D

L

I

N

S

L

L

T

S

L

L

T

D

Q

P

G

G

E

E

I

I

T

L

A

F

L

I

G

G

P

A

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
|
T

L

L

R

R

T

C

I

L

A

A

G

G

L

F

Q

E

D

Q

I

P

S

D

A

S

G

G

S

E

I

I

A

S

I

L

N

S

G

G

K

K

T

T

V

I

S

F

A

S

D

K

G

N

C

T

F

N

V

L

A

P

P

V

E

R

K

E

R

R

S

R

I

L

G

G

M

Y

I

L

F

V

Q
|
Q

D

E

Y

G

A

V

L

L

F

F

P

P

H

H

L

L

T

T

V

V

A

Y

D

R

N

N

I

I

L

A

F

Y

G

G

V

L

-

G

-

N

-

G

R

K

Q

G

L

R

D

T

K

A

Q

Q

S

E

R

R

S

Q

V

-

R

R

L

I

E

E

E

A

M

M

L

L

S

E

L

L

V

T

K

G

L

I

E

S

G

E

L

L

G

A

K

G

R
|
R

Y

Y

P

P

H

H

E
|
E

L

L

S
|
S

G
|
G

Q
|
Q

Q

Q

R

R

V

A

S

A

I

L

A

A

R

R

A

A

L

L

A

A

Y

P

E

D

P

P

D

E

L

L

L

I

L

L

D

D

E

E

P

P

F

F

S

S

N

A

I

L

D

D

A

E

Q

Q

V

L

R

R

A

Q

L

I

M

R

L

E

E

D

I

M

R

I

A

A

I

A

L

L

K

R

A

A

R

N

N

G

V

K

S

S

A

A

V

V

F

F

V

V

T

S

H

H

S

D

K

R

D

E

E

E

A

A

F

L

A

Q

F

Y

A

A

D

D

T

R

L

I

A

A

L

V

F

M

E

K

A

Q

G

G

R

R

I

I

V

L

Q

Q

H

T

G

A

I

S

P

P

E

H

T

E

L

L

Y

Y

Q

R

S

Q

P

P

N

A

T

D

P

L

Y

D

V

A

A

A

D

L

F

F

L

I

G

G

A

E

S

G

N

I

Y

V

L

F

D

P

V

A

R

A

L

L

E

N

A

A

-

D

G

G

Q

T

L

A

I

D

S

C

T

R

L

L

G

G

A

R

F

L

P

P

L

V

P

-

Q

Q

D

S

F

G

K

A

A

P

A

A

S

G

E

T

T

R

G

G

R

T

W

L

L

L

L

I

R

R

P

P

E

E

Q

Q

L

Y

L

S

I

L

E

H

A

P

R

H

A

S

D

A

G

P

A

A

G

A

E

S

I

I

binding adenosine-5'-triphosphate: F13 (≠ Y13), Q14 (≠ R14), T16 (≠ E16), V18 (= V18), S38 (= S38), G39 (= G39), C40 (= C40), G41 (= G41), K42 (= K42), T43 (= T43), T44 (= T44), Q86 (= Q86), R133 (= R131), E137 (= E135), S139 (= S137), G140 (= G138), G141 (= G139), Q142 (= Q140)
binding calcium ion: T43 (= T43), Q86 (= Q86)

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
37% identity, 84% coverage: 1:287/341 of query aligns to 1:285/393 of P9WQI3

query
sites
P9WQI3

M

M

S

A

T

E

L

I

S

V

I

L

Q

D

G

H

L

V

H

N

S

K

D

S

Y

Y

-

P

R

D

G

G

E

H

Q

T

V

A

L

V

R

R

G

D

L

L

N

N

L

L

T

T

L

I

T

A

Q

D

G

G

E

E

I

F

T

L

A

I

L

L

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

T

L

T

L

L

R

N

T

M

I

I

A

A

G

G

L

L

Q

E

D

D

I

I

S

S

A

S

G

G

S

E

I

L

A

R

I

I

N

A

G

G

K

E

T

R

V

V

S

N

A

E

D

K

G

-

C

-

F

-

V

-

A

A

P

P

E

K

K

D

R

R

S

D

I

I

G

A

M

M

I

V

F

F

Q

Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

L

M

T

T

V

V

A

R

D

Q

N

N

I

I

L

A

F

F

G

P

V

L

R

T

-

L

-

A

Q

K

L

M

D

R

K

K

Q

A

S

D

R

I

S

A

V

Q

R

K

L

V

E

S

E

E

M

T

L

A

S

K

L

I

V

L

K

D

L

L

E

T

G

N

L

L

G

L

K

D

R

R

Y

K

P

P

H

S

E

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

S

A

I

M

A

G

R

R

A

A

L

I

A

V

Y

R

E

H

P

P

D

K

L

A

L

F

L

L

L

M

D

D

E

E

P

P

F

L

S

S

N

N

I

L

D

D

A

A

Q

K

V

L

R

R

R

V

A

Q

L

M

M

R

L

G

E

E

I

I

R

A

A

Q

I

L

L

Q

K

R

A

R

R

L

N

G

V

T

S

T

A

T

V

V

F

Y

V

V

T

T

H
|
H

S

D

K

Q

D

T

E

E

A

A

F

M

A

T

F

L

A

G

D

D

T

R

L

V

A

V

L

V

F

M

E

Y

A

G

G

G

R

I

I

A

V

Q

Q

Q

H

I

G

G

I

T

P

P

E

E

T

E

L

L

Y

Y

Q

E

S

R

P

P

N

A

T

N

P

L

Y

F

V

V

A

A

D

G

F

F

L

I

G

G

-

S

-

P

A

A

S

M

N

N

Y

F

L

F

D

P

V

A

R

R

L

L

E

T

A

A

G

I

Q

G

L

L

I

T

S

L

T

P

L

F

G

G

A

E

F

V

P

T

L

L

P

A

Q

P

D

E

F

V

K

Q

A

G

A

V

S

I

E

A

T

A

G

H

R

P

W

-

L

-

R

K

P

P

E

E

Q

N

L

V

L

I

I

V

E

G

A

V

R

R

A

P

D

E

H193 (= H194) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
39% identity, 75% coverage: 19:275/341 of query aligns to 21:295/353 of 1oxvD

query
sites
1oxvD

L

L

R

D

G

N

L

V

N

N

L

I

T

N

L

I

T

E

Q

N

G

G

E

E

I

R

T

F

A

G

L

I

L

L

G

G

P
|
P

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

T
|
T

L

F

L

M

R

R

T

I

I

I

A

A

G

G

L

L

Q

D

D

V

I

P

S

S

A

T

G

G

S

E

I

L

A

Y

I

F

N

D

G

D

K

R

T

L

V

V

S

A

A

S

D

N

G

G

-

K

C

L

F

I

V

V

A

P

P

P

E

E

K

D

R

R

S

K

I

I

G

G

M

M

I

V

F

F

Q
|
Q

D

T

Y

W

A

A

L

L

F

Y

P

P

H

N

L

L

T

T

V

A

A

F

D

E

N

N

I

I

L

A

F

F

G

P

V

L

R

T

-

N

-

M

Q

K

L

M

D

S

K

K

Q

E

S

E

R

I

S

R

V

K

R

R

L

V

E

E

M

V

L

A

S

K

L

I

V

L

K

D

L

I

E

H

G

H

L

V

G

L

K

N

R

H

Y

F

P

P

H

R

E

E

L

L

S

S

G

G

Q

Q

R

R

V

V

S

A

I

L

A

A