SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 6936507 FitnessBrowser__SB2B:6936507 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

5ys3A 1.8 angstrom crystal structure of succinate-acetate permease from citrobacter koseri (see paper)
61% identity, 92% coverage: 4:187/200 of query aligns to 1:185/188 of 5ys3A

query
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5ys3A

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binding (2s)-2,3-dihydroxypropyl(7z)-pentadec-7-enoate: G103 (= G105), A132 (≠ F134), I139 (≠ F141)

5ys8A 2.8 angstrom crystal structure of succinate-acetate permease from citrobacter koseri (see paper)
62% identity, 90% coverage: 5:184/200 of query aligns to 1:181/182 of 5ys8A

query
sites
5ys8A

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binding acetate ion: T16 (= T20), F59 (= F63), F64 (= F68), F70 (= F74), W71 (= W75), F123 (= F126), F130 (= F133)

P0AC98 Succinate-acetate/proton symporter SatP; Succinate-acetate transporter protein from Escherichia coli (strain K12) (see paper)
61% identity, 93% coverage: 2:186/200 of query aligns to 3:188/188 of P0AC98

query
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P0AC98

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L131 (= L129) mutation to V: Acquires ability to transport lactic acid.
A164 (= A162) mutation to G: Acquires ability to transport lactic acid.

P41943 Glyoxylate pathway regulator from Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica) (see paper)
31% identity, 86% coverage: 2:172/200 of query aligns to 74:251/270 of P41943

query
sites
P41943

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S74 (= S2) mutation S->A,Q: No effect on sensitivity of cells to acetic acid.; mutation S->H,D: No effect on sensitivity of cells to acetic acid.

Sites not aligning to the query:

58 mutation Y->E,F: No effect on sensitivity of cells to acetic acid.

Query Sequence

>6936507 FitnessBrowser__SB2B:6936507
MSTPLANPAPLGLMGFGMTTILLNIHNAGFFPIDAMILAMGIFYGGLGQVIVGIMCFMRG
DTFGTTAFTSYGLFWLTLVGLILMPNAGVAASPASFMGWYLALWGVFTGFMFIGSLRYAR
IKQFIFGSLTLLFFLLAARDFTGSELIGTIAAWEGIICGASAIYFAMAQVINGEYGRTVL
PVGERKVKAPVVAVSEAKAA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory