SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 6936601 FitnessBrowser__SB2B:6936601 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3oceA Crystal structure of fumarate lyase:delta crystallin from brucella melitensis bound to cobalt
58% identity, 97% coverage: 3:462/472 of query aligns to 2:460/461 of 3oceA

query
sites
3oceA

T

T

R

R

I

R

E

E

H

Q

D

D

L

S

L

L

G

G

D

E

A

R

P

D

V

I

P

P

A

M

Q

D

A

A

W

Y

Y

F

G

G

I

I

Q

Q

T

T

Q

L

R

R

A

A

L

V

E

E

N

N

F

F

S

S

L

L

S

S

G

D

T

V

P

A

I

L

N

N

A

H

F

I

P

P

E

A

L

L

I

V

R

R

A

A

L

L

A

A

K

M

V

V

K

K

A

K

A

A

A

A

A

A

R

T

A

A

N

N

Q

Y

S

K

L

L

G

R

Q

Q

L

L

S

P

D

E

I

P

K

K

A

Y

N

A

A

A

I

I

A

V

A

A

A

A

C

C

D

D

D

D

I

I

V

I

Q

D

G

G

A

L

L

L

H

M

D

E

Q

Q

F

F

V

V

V

V

D

D

L

V

I

F

Q

Q

G

G

G

G

A

A

G

G

T

T

S

S

T

S

N

N

M

M

N

N

A

A

N

N

E

E

V

V

I

I

A

A

N

N

L

R

A

A

L

L

A

E

K

H

L

L

G

G

H

R

G

P

K

R

G

G

E

D

Y

Y

R

Q

H

T

L

I

H

H

P

P

N

N

N

D

D

D

V

V

N

N

C

M

S

S

Q

Q

S

S

T

T

N

N

D

D

A

V

Y

Y

P

P

T

T

A

A

A

V

R

R

L

L

A

A

M

L

V

L

E

L

A

S

T

Q

A

N

P

Q

L

V

K

Q

V

T

A

A

I

L

E

H

A

R

I

L

C

I

L

A

S

A

L

F

E

E

V

A

K

K

G

G

R

R

E

E

F

F

S

A

H

T

I

V

L

I

K

K

M

I

G

G

R

R

T

T

Q
|
Q

L

L

Q

Q

D

D

A

A

V

V

P

P

M

I

T

T

L

L

G

G

Q

Q

E

E

F

F

D

E

A

A

F

F

A

A

S

A

S

T

L

L

K

R

S

E

D

D

I

T

G

A

R

R

I

L

D

E

D

E

A

V

C

A

K

A

E

L

L

F

C

R

V

E

V

V

N

N

L

L

G

G

G

G

T

T

A

A

I

I

G

G

T

T

G

R

I

I

N

N

T

A

H

S

P

H

A

A

Y

Y

G

A

V

E

L

Q

A

A

V

I

K

V

A

E

L

L

A

S

D

Q

I

I

T

S

G

G

L

I

P

E

V

L

T

K

Q

A

A

T

D

G

N

N

L

L

I

V

D
x
E

A

A

T

S

T

W

D
|
D

M

T

G

G

A

A

F

F

V

V

T

T

L

F

S

S

S

G

V

I

L

L

K

R

R

R

L

I

A

A

V

V

K

K

L

L

S

S

K

K

L

I

S

A

N

N

D

D

L

L

R

R

L

L

L

L

S

S

S

S

G

G

P

P

R

R

T

S

G

G

L

L

G

G

E

E

I

I

R

R

L

L

P

P

A

A

M

V

Q

Q

P

P

G

G

S
|
S

S

S

I

I

M

M

P

P

G

G

K
|
K

V

V

N

N

P

P

V

V

I

I

P

P

E
|
E

A

S

V

V

N

N

Q

Q

V

V

A

C

F

Y

Q

Q

V

V

I

I

G

G

T

N

D

D

M

L

T

T

I

V

T

T

M

M

A

A

A

A

E

E

A

S

A

G

Q

Q

L

L

Q

Q

L

L

N

N

A

A

M

F

E

E

P

P

V

L

I

I

V

V

Y

Y

N

N

L

I

L

L

N

S

N

S

C

M

A

R

L

L

L

L

T

G

R

R

A

A

I

M

A

T

M

N

L

L

D

A

S

E

R

R

C

C

I

V

Q

D

G

G

I

I

E

E

A

A

N

N

E

V

A

E

K

R

C

C

E

R

Q

A

H

G

V

A

S

E

S

E

S

S

I

I

G

S

I

L

V

A

T

T

A

A

L

L

V

V

P

P

H

V

I

V

G

G

Y

Y

E

A

N

R

A

A

T

A

R

E

I

I

A

A

G

K

E

Q

A

A

L

L

L

A

S

S

G

G

A

Q

G

T

V

V

A

M

E

E

L

V

V

A

R

I

R

S

D

K

G

G

L

-

L

L

S

D

D

A

D

S

A

A

L

L

S

T

Q

I

I

M

L

L

S

D

P

P

A

L

A

R

M

M

V

A

T

F

P

P

active site: Q187 (= Q188), S317 (= S318), K323 (= K324), E330 (= E331)
binding cobalt (ii) ion: E265 (≠ D266), D269 (= D270)

6wngA Crystal structure of an aspartate ammonia-lyase from elizabethkingia anophelis nuhp1
55% identity, 98% coverage: 2:462/472 of query aligns to 2:462/466 of 6wngA

query
sites
6wngA

D

D

T

Y

R

R

I

I

E

E

H

S

D

D

L

L

L

I

G

G

D

E

A

L

P

K

V

V

P

P

A

V

Q

N

A

A

W

Y

Y

Y

G

G

I

V

Q

Q

T

T

Q

Q

R

R

A

A

L

I

E

D

N

N

F

F

S

K

L

I

S

S

G

N

T

D

P

H

I

L

N

S

A

D

F

H

P

P

E

E

L

F

I

I

R

K

A

A

L

F

A

A

K

F

V

V

K

K

A

K

A

A

A

A

A

A

R

Q

A

T

N

N

Q

F

S

E

L

L

G

G

Q

L

L

L

S

D

D

E

I

I

K

I

A

N

N

K

A

N

I

I

A

A

A

T

A

A

C

C

D

D

D

E

I

I

V

I

Q

A

G

G

A

K

L

M

H

H

D

K

Q

E

F

F

V

P

V

T

D

D

L

M

I

I

Q

Q

G

G

G

G

A

A

G

G

T
|
T

S

S

T

M

N

N

M

M

N

N

A

A

N

N

E

E

V

V

I

I

A

A

N

N

L

R

A

A

L

L

A

E

K

L

L

M

G

G

H

H

G

Q

K

K

G

G

E

E

Y

Y

R

Q

H

F

L

C

H

S

P

P

N

N

N

D

D

H

V

V

N

N

C

L

S

S

Q

Q

S
|
S

T
|
T

N
|
N

D

D

A

A

Y

Y

P

P

T

T

A

A

A

I

R

R

L

I

A

A

M

L

V

Y

E

N

A

L

T

N

A

K

P

T

L

L

K

V

V

E

A

R

I

L

E

E

A

L

I

L

C

I

L

Q

S

S

L

F

E

R

V

K

K

K

G

A

R

D

E

D

F

L

S

K

H

D

I

V

L

I

K

K

M

M

G

G

R

R

T
|
T

Q
|
Q

L

L

Q

Q

D

D

A

A

V

V

P

P

M

M

T

T

L

M

G

G

Q

Q

E

E

F

F

D

N

A

A

F

F

A

A

S

N

S

T

L

L

K

Q

S

E

D

E

I

I

G

A

R

R

I

L

D

N

D

T

A

N

C

A

K

D

E

L

L

F

C

L

V

E

V

T

N

N

L

M

G

G

G

A

T

T

A

A

I

I

G

G

T

T

G

G

I

L

N

N

T

A

H

H

P

P

A

D

Y

Y

G

A

V

V

L

K

A

C

V

T

K

E

A

N

L

L

A

A

D

K

I

I

T

S

G

G

L

A

P

D

V

V

T

V

Q

L

A

A

D

S

N

D

L

L

I

V

D

E

A

A

T

T

T

P

D

D

M

T

G

G

A

A

F

Y

V

V

T

I

L

Y

S

S

S

S

V

A

L

M

K

K

R

R

L

M

A

A

V

V

K

K

L

L

S

S

K

K

L

I

S

C

N

N

D

D

L

L

R

R

L

L

L

L

S

A

S

S

G

G

P

P

R

R

T

A

G

G

L

L

G

Y

E

E

I

I

R

N

L

L

P

P

A

K

M

M

Q

Q

P

P

G
|
G

S
|
S

S
|
S

I
|
I

M
|
M

P

P

G

G

K
|
K

V

V

N
|
N

P

P

V

V

I

I

P

P

E

E

A

V

V

V

N

N

Q

Q

V

V

A

C

F

F

Q

K

V

V

I

I

G

G

T

N

D

D

M

L

T

T

I

V

T

T

M

F

A

A

A

A

E

E

A

A

A

G

Q

Q

L

L

Q

Q

L

L

N

N

A

V

M

M

E

E

P

P

V

V

I

L

V

T

Y

Q

N

S

L

I

L

M

N

E

N

S

C

I

A

R

L

F

L

L

T

K

R

N

A

A

I

M

A

D

M

T

L

L

D

R

S

E

R

K

C

C

I

I

Q

D

G

G

I

I

E

T

A

A

N

N

E

K

A

E

K

I

C

C

E

L

Q

N

H

M

V

V

S

K

S

N

S

S

I

I

G

G

I

I

V

V

T

T

A

A

L

L

V

N

P

P

H

Y

I

I

G

G

Y

Y

E

K

N

N

A

S

T

T

R

K

I

I

A

A

G

K

E

E

A

A

L

L

L

D

S

T

G

G

A

K

G

S

V

V

A

Y

E

D

L

L

V

V

R

L

R

E

D

H

G

E

L

L

L

L

S

S

D

K

D

E

A

K

L

L

S

D

Q

E

I

I

L

L

S

A

P

P

A

E

A

N

M

M

V

L

T

N

P

P

binding fumaric acid: T101 (= T101), S140 (= S140), T141 (= T141), N142 (= N142), T187 (= T187), Q188 (= Q188), G317 (= G317), S318 (= S318), S319 (= S319), I320 (= I320), M321 (= M321), K324 (= K324), N326 (= N326)

3r6vG Crystal structure of aspartase from bacillus sp. Ym55-1 with bound l- aspartate (see paper)
49% identity, 98% coverage: 2:462/472 of query aligns to 1:459/463 of 3r6vG

query
sites
3r6vG

D

D

T

V

R

R

I

I

E

E

H

K

D

D

L

F

L

L

G

G

D

E

A

K

P

E

V

I

P

P

A

K

Q

D

A

A

W

Y

Y

Y

G

G

I

V

Q

Q

T

T

Q

I

R

R

A

A

L

T

E

E

N

N

F

F

S

P

L

I

S

T

G

G

T

Y

P

R

I

I

N

H

A

-

F

-

P

P

E

E

L

L

I

I

R

K

A

S

L

L

A

G

K

I

V

V

K

K

A

K

A

S

A

A

A

A

R

L

A

A

N

N

Q

M

S

E

L

V

G

G

Q

L

L

L

S

D

D

K

I

E

K

V

A

G

N

Q

A

Y

I

I

A

V

A

K

A

A

C

A

D

D

D

E

I

V

V

I

Q

E

G

G

A

K

L

W

H

N

D

D

Q

Q

F

F

V

I

V

V

D

D

L

P

I

I

Q

Q

G

G

G

G

A

A

G

G

T

T

S

S

T

I

N

N

M

M

N

N

A

A

N

N

E

E

V

V

I

I

A

A

N

N

L

R

A

A

L

L

A

E

K

L

L

M

G

G

H

E

G

E

K

K

G

G

E

N

Y

Y

R

S

H

K

L

I

H

S

P

P

N

N

N

S

D

H

V

V

N

N

C

M

S

S

Q

Q

S

S

T

T

N

N

D

D

A

A

Y

F

P

P

T

T

A

A

A

T

R

H

L

I

A

A

M

V

V

L

E

S

A

L

T

L

A

N

P

Q

L

L

K

I

V

E

A

T

I

T

E

K

A

Y

I

M

C

Q

L

Q

S

E

L

F

E

M

V

K

K

K

G

A

R

D

E

E

F

F

S

A

H

G

I

V

L

I

K

K

M

M

G

G

R

R

T

T

Q
x
H

L

L

Q

Q

D

D

A

A

V

V

P

P

M

I

T

L

L

L

G

G

Q

Q

E

E

F

F

D

E

A

A

F

Y

A

A

S

R

S

V

L

I

K

A

S

R

D

D

I

I

G

E

R

R

I

I

D

A

D

N

A

T

C

R

K

N

E

N

L

L

C

Y

V

D

V

I

N

N

L

M

G

G

G

A

T

T

A

A

I

V

G

G

T

T

G

G

I

L

N

N

T

A

H

D

P

P

A

E

Y

Y

G

I

V

S

L

I

A

V

V

T

K

E

A

H

L

L

A

A

D

K

I

F

T

S

G

G

L

H

P

P

V

L

T

R

Q

S

A

A

D

Q

N

H

L

L

I

V

D

D

A

A

T

T

T

Q

D

N

M

T

G

D

A

C

F

Y

V

T

T

E

L

V

S

S

S

S

V

A

L

L

K

K

R

V

L

C

A

M

V

I

K

N

L

M

S

S

K

K

L

I

S

A

N

N

D

D

L

L

R

R

L

L

L

M

S

A

S

S

G

G

P

P

R

R

T

A

G

G

L

L

G

S

E

E

I

I

R

V

L

L

P

P

A

A

M

R

Q

Q

P

P

G
|
G

S
|
S

S
|
S

I

I

M

M

P

P

G

G

K
|
K

V

V

N
|
N

P

P

V

V

I

M

P

P

E
|
E

A

V

V

M

N

N

Q

Q

V

V

A

A

F

F

Q

Q

V

V

I

F

G

G

T

N

D

D

M

L

T

T

I

I

T

T

M

S

A

A

A

S

E

E

A

A

A

G

Q

Q

L

F

Q

E

L

L

N

N

A

V

M

M

E

E

P

P

V

V

I

L

V

F

Y

F

N

N

L

L

L

I

N

Q

N

S

C

I

A

S

L

I

L

M

T

T

R

N

A

V

I

F

A

K

M

S

L

F

D

T

S

E

R

N

C

C

I

L

Q

K

G

G

I

I

E

K

A

A

N

N

E

E

A

E

K

R

C

M

E

K

Q

E

H

Y

V

V

S

E

S

K

S

S

I

I

G

G

I

I

V

I

T

T

A

A

L

I

V

N

P

P

H

H

I

V

G

G

Y

Y

E

E

N

T

A

A

T

A

R

K

I

L

A

A

G

R

E

E

A

A

L

Y

L

L

S

T

G

G

A

E

G

S

V

I

A

R

E

E

L

L

V

C

R

I

R

K

D

Y

G

G

L

V

L

L

S

T

D

E

D

E

A

Q

L

L

S

N

Q

E

I

I

L

L

S

N

P

P

A

Y

A

E

M

M

V

I

T

H

P

P

active site: H185 (≠ Q188), S315 (= S318), K321 (= K324), E328 (= E331)
binding aspartic acid: G314 (= G317), S315 (= S318), S316 (= S319), K321 (= K324), N323 (= N326)

3r6qA A triclinic-lattice structure of aspartase from bacillus sp. Ym55-1 (see paper)
49% identity, 97% coverage: 4:462/472 of query aligns to 2:458/462 of 3r6qA

query
sites
3r6qA

R

R

I

I

E

E

H

K

D

D

L

F

L

L

G

G

D

E

A

K

P

E

V

I

P

P

A

K

Q

D

A

A

W

Y

Y

Y

G

G

I

V

Q

Q

T

T

Q

I

R

R

A

A

L

T

E

E

N

N

F

F

S

P

L

I

S

T

G

G

T

Y

P

R

I

I

N

H

A

-

F

-

P

P

E

E

L

L

I

I

R

K

A

S

L

L

A

G

K

I

V

V

K

K

A

K

A

S

A

A

A

A

R

L

A

A

N

N

Q

M

S

E

L

V

G

G

Q

L

L

L

S

D

D

K

I

E

K

V

A

G

N

Q

A

Y

I

I

A

V

A

K

A

A

C

A

D

D

D

E

I

V

V

I

Q

E

G

G

A

K

L

W

H

N

D

D

Q

Q

F

F

V

I

V

V

D

D

L

P

I

I

Q

Q

G

G

G

G

A

A

G

G

T

T

S

S

T

I

N

N

M

M

N

N

A

A

N

N

E

E

V

V

I

I

A

A

N

N

L

R

A

A

L

L

A

E

K

L

L

M

G

G

H

E

G

E

K

K

G

G

E

N

Y

Y

R

S

H

K

L

I

H

S

P

P

N

N

N

S

D

H

V

V

N

N

C

M

S

S

Q

Q

S

S

T

T

N

N

D

D

A

A

Y

F

P

P

T

T

A

A

A

T

R

H

L

I

A

A

M

V

V

L

E

S

A

L

T

L

A

N

P

Q

L

L

K

I

V

E

A

T

I

T

E

K

A

Y

I

M

C

Q

L

Q

S

E

L

F

E

M

V

K

K

K

G

A

R

D

E

E

F

F

S

A

H

G

I

V

L

I

K

K

M

M

G

G

R

R

T

T

Q
x
H

L

L

Q

Q

D

D

A

A

V

V

P

P

M

I

T

L

L

L

G

G

Q

Q

E

E

F

F

D

E

A

A

F

Y

A

A

S

R

S

V

L

I

K

A

S

R

D

D

I

I

G

E

R

R

I

I

D

A

D

N

A

T

C

R

K

N

E

N

L

L

C

Y

V

D

V

I

N

N

L

M

G

G

G

A

T

T

A

A

I

V

G

G

T

T

G

G

I

L

N

N

T

A

H

D

P

P

A

E

Y

Y

G

I

V

S

L

I

A

V

V

T

K

E

A

H

L

L

A

A

D

K

I

F

T

S

G

G

L

H

P

P

V

L

T

R

Q

S

A

A

D

Q

N

H

L

L

I

V

D

D

A

A

T

T

T

Q

D

N

M

T

G

D

A

C

F

Y

V

T

T

E

L

V

S

S

S

S

V

A

L

L

K

K

R

V

L

C

A

M

V

I

K

N

L

M

S

S

K

K

L

I

S

A

N

N

D

D

L

L

R

R

L

L

L

M

S

A

S

S

G

G

P

P

R

R

T

A

G

G

L

L

G

S

E

E

I

I

R

V

L

L

P

P

A

A

M

R

Q

Q

P

P

G

G

S
|
S

S

S

I

I

M

M

P

P

G

G

K
|
K

V

V

N

N

P

P

V

V

I

M

P

P

E
|
E

A

V

V

M

N

N

Q

Q

V

V

A

A

F

F

Q

Q

V

V

I

F

G

G

T

N

D

D

M

L

T

T

I

I

T

T

M

S

A

A

A

S

E

E

A

A

A

G

Q

Q

L

F

Q

E

L

L

N

N

A

V

M

M

E

E

P

P

V

V

I

L

V

F

Y

F

N

N

L

L

L

I

N

Q

N

S

C

I

A

S

L

I

L

M

T

T

R

N

A

V

I

F

A

K

M

S

L

F

D

T

S

E

R

N

C

C

I

L

Q

K

G

G

I

I

E

K

A
|
A

N

N

E
|
E

A

E

K

R

C

M

E

K

Q

E

H

Y

V

V

S

E

S

K

S

S

I

I

G

G

I

I

V

I

T

T

A

A

L

I

V

N

P

P

H

H

I

V

G

G

Y

Y

E

E

N

T

A

A

T

A

R

K

I

L

A

A

G

R

E

E

A

A

L

Y

L

L

S

T

G

G

A

E

G

S

V

I

A

R

E

E

L

L

V

C

R

I

R

K

D

Y

G

G

L

V

L

L

S

T

D

E

D

E

A

Q

L

L

S

N

Q

E

I

I

L

L

S

N

P

P

A

Y

A

E

M

M

V

I

T

H

P

P

active site: H184 (≠ Q188), S314 (= S318), K320 (= K324), E327 (= E331)
binding calcium ion: A389 (= A393), E391 (= E395)

P0AC38 Aspartate ammonia-lyase; Aspartase; EC 4.3.1.1 from Escherichia coli (strain K12) (see 2 papers)
54% identity, 98% coverage: 2:462/472 of query aligns to 4:465/478 of P0AC38

query
sites
P0AC38

D

N

T

I

R

R

I

I

E

E

H

E

D
|
D

L

L

L

L

G

G

D

T

A
x
R

P

E

V

V

P

P

A

A

Q

D

A

A

W

Y

Y

Y

G

G

I

V

Q
x
H

T

T

Q

L

R
|
R

A

A

L

I

E

E

N

N

F

F

S

Y

L

I

S

S

G

N

T

N

P

K

I

I

N

S

A

D

F

I

P

P

E

E

L

F

I

V

R

R

A

G

L

M

A

V

K

M

V

V

K
|
K

A

K

A

A

A

A

A

A

R

M

A

A

N

N

Q

K

S

E

L

L

G

Q

Q

T

L

I

S

P

D

K

I

S

K

V

A

A

N

N

A

A

I

I

A

I

A

A

A

A

C

C

D

D

D

E

I

V

V

L

Q

N

-

N

G

G

A

K

L

C

H

M

D

D

Q

Q

F

F

V

P

V

V

D

D

L

V

I

Y

Q

Q

G

G

G

G

A

A

G

G

T

T

S

S

T

V

N

N

M

M

N

N

A

T

N

N

E

E

V

V

I

L

A

A

N

N

L

I

A

G

L

L

A

E

K

L

L

M

G

G

H
|
H

G

Q

K

K

G

G

E

E

Y

Y

R

Q

H

Y

L

L

H

N

P

P

N

N

N

D

D

H

V

V

N

N

C

K

S

C

Q

Q

S
|
S

T

T

N

N

D

D

A

A

Y

Y

P

P

T

T

A

G

A

F

R

R

L

I

A

A

M

V

V

Y

E

S

A

S

T

L

A

I

P

K

L

L

K

V

V

D

A

A

I

I

E

N

A

Q

I

L

C

R

L

E

S

G

L

F

E

E

V

R

K

K

G

A

R

V

E

E

F

F

S

Q

H

D

I

I

L

L

K

K

M

M

G

G

R

R

T

T

Q

Q

L

L

Q

Q

D

D

A

A

V

V

P

P

M

M

T

T

L

L

G

G

Q

Q

E

E

F

F

D

R

A

A

F

F

A

S

S

I

S

L

L

L

K

K

S

E

D

E

I

V

G

K

R

N

I

I

D

Q

D

R

A

T

C

A

K

E

E

L

L

L

C

L

V

E

V

V

N

N

L

L

G

G

G

A

T

T

A

A

I

I

G

G

T

T

G

G

I

L

N

N

T

T

H

P

P

K

A

E

Y

Y

G

S

V

P

L

L

A

A

V

V

K

K

A

K

L

L

A

A

D

E

I

V

T

T

G

G

L

F

P

P

V

C

T

V

Q

P

A

A

D

E

N

D

L

L

I

I

D

E

A

A

T

T

T

S

D

D

M

C

G

G

A

A

F

Y

V

V

T

M

L

V

S

H

S

G

V

A

L

L

K

K

R

R

L

L

A

A

V

V

K

K

L

M

S

S

K

K

L

I

S

C

N

N

D

D

L

L

R

R

L

L

L

L

S

S

S

S

G

G

P

P

R

R

T

A

G

G

L

L

G

N

E

E

I

I

R

N

L

L

P

P

A

E

M

L

Q

Q

P

A

G

G

S

S

S

S

I

I

M

M

P

P

G

A

K
|
K

V

V

N

N

P

P

V

V

I

V

P

P

E

E

A

V

V

V

N

N

Q

Q

V

V

A

C

F

F

Q

K

V

V

I

I

G

G

T

N

D

D

M

T

T

T

I

V

T

T

M

M

A

A

A

A

E

E

A

A

A

G

Q

Q

L

L

Q

Q

L

L

N

N

A

V

M

M

E

E

P

P

V

V

I

I

V

G

Y

Q

N

A

L

M

L

F

N

E

N

S

C

V

A

H

L

I

L

L

T

T

R

N

A

A

I

C

A

Y

M

N

L

L

D

L

S

E

R

K

C

C

I

I

Q

N

G

G

I

I

E

T

A

A

N

N

E

K

A

E

K

V

C

C

E

E

Q

G

H

Y

V

V

S

Y

S

N

S

S

I

I

G

G

I

I

V

V

T

T

A

Y

L

L

V

N

P

P

H

F

I

I

G

G

Y

H

E

H

N

N

A

G

T

D

R

I

I

V

A

G

G

K

E

I

A

C

L

A

L

E

S

T

G

G

A

K

G

S

V

V

A

R

E

E

L

V

V

V

R

L

R

E

D

R

G

G

L

L

L

L

S

T

D

E

D

A

A

E

L

L

S

D

Q

D

I

I

L

F

S

S

P

V

A

Q

A

N

M

L

V

M

T

H

P

P

D10 (= D8) mutation to A: Loss of activity.; mutation to N: Reduces Kcal by 80%. Increases KM for aspartic acid 3-fold.
R15 (≠ A13) mutation to A: No effect on kcat. Increases KM for aspartic acid 2.5-fold.
H26 (≠ Q24) mutation to N: No effect on kcat. Increases KM for aspartic acid 3-fold.
R29 (= R27) mutation to A: No effect on kcat. Increases KM for aspartic acid 40-fold.
K55 (= K53) mutation to R: Loss of activity.
H124 (= H121) mutation to L: Reduces activity by 30%.
S143 (= S140) mutation to G: Reduces kcat by 90%. Increases KM for aspartic acid 4-fold.; mutation to T: Reduces kcat by 98.5%. Increases KM for aspartic acid 2-fold.
K327 (= K324) mutation to R: Reduces activity by 99.7%. Increases KM for aspartic acid 5-fold.

1jswA Native l-aspartate ammonia lyase (see paper)
55% identity, 96% coverage: 2:455/472 of query aligns to 4:458/459 of 1jswA

query
sites
1jswA

D

N

T

I

R

R

I

I

E

E

H

E

D

D

L

L

L

L

G

G

D

T

A

R

P

E

V

V

P

P

A

A

Q

D

A

A

W

Y

Y

Y

G

G

I

V

Q

H

T

T

Q

L

R

R

A

A

L

I

E

E

N

N

F

F

S

Y

L

I

S

S

G

N

T

N

P

K

I

I

N

S

A

D

F

I

P

P

E

E

L

F

I

V

R

R

A

G

L

M

A

V

K

M

V

V

K

K

A

K

A

A

A

A

A

A

R

M

A

A

N

N

Q

K

S

E

L

L

G

Q

Q

T

L

I

S

P

D

K

I

S

K

V

A

A

N

N

A

A

I

I

A

I

A

A

A

A

C

C

D

D

D

E

I

V

V

L

Q

N

-

N

G

G

A

K

L

C

H

M

D

D

Q

Q

F

F

V

P

V

V

D

D

L

V

I

Y

Q

Q

G

G

G

G

A

A

G

G

T

T

S

S

T

V

N

N

M

M

N

N

A

T

N

N

E

E

V

V

I

L

A

A

N

N

L

I

A

G

L

L

A

E

K

L

L

M

G

G

H

H

G

Q

K

K

G

G

E

E

Y

Y

R

Q

H

Y

L

L

H

N

P

P

N

N

N

D

D

H

V

V

N

N

C

K

S

C

Q

Q

S

S

T

T

N

N

D

D

A

A

Y

Y

P

P

T

T

A

G

A

F

R

R

L

I

A

A

M

V

V

Y

E

S

A

S

T

L

A

I

P

K

L

L

K

V

V

D

A

A

I

I

E

N

A

Q

I

L

C

R

L

E

S

G

L

F

E

E

V

R

K

K

G

A

R

V

E

E

F

F

S

Q

H

D

I

I

L

L

K

K

M

M

G

G

R

R

T

T

Q
|
Q

L

L

Q

Q

D

D

A

A

V

V

P

P

M

M

T

T

L

L

G

G

Q

Q

E

E

F

F

D

R

A

A

F

F

A

S

S

I

S

L

L

L

K

K

S

E

D

E

I

V

G

K

R

N

I

I

D

Q

D

R

A

T

C

A

K

E

E

L

L

L

C

L

V

E

V

V

N

N

L

L

G

G

G

A

T

T

A

A

I

I

G

G

T

T

G

G

I

L

N

N

T

T

H

P

P

K

A

E

Y

Y

G

S

V

P

L

L

A

A

V

V

K

K

A

K

L

L

A

A

D

E

I

V

T

T

G

G

L

F

P

P

V

C

T

V

Q

P

A

A

D

E

N

D

L

L

I

I

D

E

A

A

T

T

T

S

D

D

M

C

G

G

A

A

F

Y

V

V

T

M

L

V

S

H

S

G

V

A

L

L

K

K

R

R

L

L

A

A

V

V

K

K

L

M

S

S

K

K

L

I

S

C

N

N

D

D

L

L

R

R

L

L

L

L

S

S

S

S

G

G

P

P

R

R

T

A

G

G

L

L

G

N

E

E

I

I

R

N

L

L

P

P

A

E

M

L

Q

Q

P

A

G

G

S
|
S

S

S

I

I

M

M

P

P

G

A

K
|
K

V

V

N

N

P

P

V

V

I

V

P

P

E
|
E

A

V

V

V

N

N

Q

Q

V

V

A

C

F

F

Q

K

V

V

I

I

G

G

T

N

D

D

M

T

T

T

I

V

T

T

M

M

A

A

A

A

E

E

A

A

A

G

Q

Q

L

L

Q

Q

L

L

N

N

A

V

M

M

E

E

P

P

V

V

I

I

V

G

Y

Q

N

A

L

M

L

F

N

E

N

S

C

V

A

H

L

I

L

L

T

T

R

N

A

A

I

C

A
x
Y

M

N

L

L

D

L

S
x
E

R

K

C

C

I

I

Q

N

G

G

I

I

E

T

A

A

N

N

E

K

A

E

K

V

C

C

E

E

Q

G

H

Y

V

V

S

Y

S

N

S

S

I

I

G

G

I

I

V

V

T

T

A

Y

L

L

V

N

P

P

H

F

I

I

G

G

Y

H

E

H

N

N

A

G

T

D

R

I

I

V

A

G

G

K

E

I

A

C

L

A

L

E

S

T

G

G

A

K

G

S

V

V

A

R

E

E

L

V

V

V

R

L

R

E

D

R

G

G

L

L

L

L

S

T

D

E

D

A

A

E

L

L

S

D

Q

D

I

I

L

F

S

S

active site: Q191 (= Q188), S321 (= S318), K327 (= K324), E334 (= E331)
binding beta-D-glucopyranose: Y384 (≠ A381), E388 (≠ S385)

7c18B Crystal structure of fumarasec from mannheimia succiniciproducens in complex with fumarate
43% identity, 97% coverage: 4:460/472 of query aligns to 4:460/464 of 7c18B

query
sites
7c18B

R

R

I

I

E

E

H

K

D

D

L

T

L

M

G

G

D

E

A

V

P

Q

V

V

P

P

A

A

Q

D

A

K

W

Y

Y

W

G

A

I

A

Q

Q

T

T

Q

E

R

R

A

S

L

R

E

N

N

N

F

F

S

K

L

I

S

G

G

-

T

-

P

P

I

A

N

A

A

S

F

M

P

P

-

H

E

E

L

I

I

I

R

E

A

A

L

F

A

G

K

Y

V

L

K

K

A

K

A

A

A

A

A

A

R

F

A

A

N

N

Q

T

S

D

L

L

G

G

Q

V

L

L

S

P

D

A

I

E

K

K

A

R

N

D

A

L

I

I

A

G

A

Q

A

A

C

C

D

D

D

E

I

I

V

L

Q

A

G

R

A

K

L

L

H

D

D

D

Q

Q

F

F

V

P

V

L

D

V

L

I

I

W

Q

Q

G

T

G

G

A

S

G

G

T
|
T

S

Q

T

S

N

N

M

M

N

N

A

L

N

N

E

E

V

V

I

I

A

A

N

N

L

R

A

A

L

H

A

V

K

I

L

N

G

G

H

G

G

K

K

L

G

G

E

E

Y

K

R

S

H

I

L

I

H

H

P

P

N

N

N

D

D

D

V

V

N

N

C

K

S

S

Q

Q

S
|
S

T
x
S

N
|
N

D

D

A

T

Y

Y

P

P

T

T

A

A

A

M

R

H

L

I

A

A

-

A

-

Y

-

K

-

K

M

V

V

V

E

E

A

A

T

T

A

I

P

P

L

-

K

-

V

-

A

A

I

V

E

E

A

R

I

L

C

Q

L

K

S

T

L

L

E

A

V

A

K

K

G

A

R

A

E

E

F

F

S

K

H

D

I

V

L

V

K

K

M

I

G

G

R

R

T
|
T

Q
x
H

L

L

Q

M

D

D

A

A

V

T

P

P

M

L

T

T

L

L

G

G

Q

Q

E

E

F

F

D

S

A

G

F

Y

A

A

S

A

S

Q

L

L

K

S

S

F

D

G

I

L

G

T

R

A

I

I

D

K

D

N

A

T

C

L

K

P

E

H

L

L

C

R

V

Q

V

L

N

A

L

L

G

G

G

G

T

T

A

A

I

V

G

G

T

T

G

G

I

L

N

N

T

T

H

P

P

K

A

G

Y

Y

G

D

V

V

L

K

A

V

V

A

K

E

A

Y

L

I

A

A

D

K

I

F

T

T

G

G

L

L

P

P

V

F

T

I

Q

T

A

A

D

E

N

N

L

K

I

F

D

E

A

A

T

L

T

A

D

T

M

H

G

D

A

A

F

I

V

V

T

E

L

T

S

H

S

G

V

A

L

L

K

K

R

Q

L

V

A

A

V

M

K

S

L

L

S

F

K

K

L

I

S

A

N

N

D

D

L

I

R

R

L

L

L

L

S

A

S

S

G

G

P

P

R

R

T

S

G

G

L

I

G

G

E

E

I

I

R

L

L

I

P

P

A

E

M

N

Q

E

P

P

G
|
G

S
x
C

S
|
S

I
|
I

M
|
M

P

P

G

G

K
|
K

V

V

N
|
N

P

P

V

T

I

Q

P

C

E

E

A

A

V

M

N

T

Q

M

V

V

A

A

F

A

Q

Q

V

V

I

L

G

G

T

N

D

D

M

T

T

T

I

I

T

S

M

F

A

A

A

G

E

S

A

Q

A

G

Q

H

L

F

Q

E

L

L

N

N

A

V

M

F

E

K

P

P

V

V

I

M

V

A

Y

A

N

N

L

F

L

L

N

Q

N

S

C

A

A

Q

L

L

L

I

T

A

R

D

A

V

I

C

A

I

M

S

L

F

D

D

S

E

R

H

C

C

I

A

Q

T

G

G

I

I

E

Q

A

P

N

N

E

T

A

P

K

R

C

I

E

Q

Q

H

H

L

V

L

S

D

S

S

S

S

I

L

G

M

I

L

V

V

T

T

A

A

L

L

V

N

P

T

H

H

I

I

G

G

Y

Y

E

E

N

N

A

A

T

A

R

K

I

I

A

A

G

K

E

T

A

A

L

H

L

K

S

N

G

G

A

T

G

T

V

L

A

R

E

E

L

E

V

A

R

I

R

N

D

L

G

G

L

L

L

V

S

S

D

A

D

E

A

D

L

F

S

D

Q

K

I

W

L

V

S

V

P

P

A

A

A

D

M

M

V

V

binding fumaric acid: T100 (= T101), S139 (= S140), S140 (≠ T141), N141 (= N142), T187 (= T187), H188 (≠ Q188), G317 (= G317), C318 (≠ S318), S319 (= S319), I320 (= I320), M321 (= M321), K324 (= K324), N326 (= N326)

Q9ZCQ4 Fumarate hydratase class II; Fumarase C; Aerobic fumarase; Iron-independent fumarase; EC 4.2.1.2 from Rickettsia prowazekii (strain Madrid E) (see paper)
41% identity, 97% coverage: 2:461/472 of query aligns to 3:461/461 of Q9ZCQ4

query
sites
Q9ZCQ4

D

N

T

Y

R

R

I

I

E

E

H

S

D

D

L

S

L

F

G

G

D

E

A

I

P

Q

V

I

P

E

A

E

Q

K

A

F

W

Y

Y

W

G

G

I

A

Q

Q

T

T

Q

Q

R

R

A

S

L

L

E

N

N

N

F

F

S

K

L

I

S

S

G

K

T

Q

P

K

I

M

N

P

A

K

F

I

P

-

E

-

L

L

I

I

R

R

A

A

L

L

A

A

K

I

V

L

K

K

A

K

A

C

A

A

A

A

R

Q

A

V

N

N

Q

Y

S

E

L

F

G

G

Q

D

L

L

S

E

D

Y

I

K

K

I

A

A

N

T

A

S

I

I

A

D

A

K

A

A

C

I

D

D

D

R

I

I

V

L

Q

A

G

G

A

E

L

F

H

E

D

D

Q

N

F

F

V

P

V

L

D

V

L

V

I

W

Q

Q

G

T

G

G

A
x
S

G
|
G

T
|
T

S

Q

T

T

N

N

M

M

N

N

A

M

N

N

E

E

V

V

I

I

A

A

N

S

L

I

A

A

L

N

A

E

K

E

L

L

G

T

H

G

G

K

K

K

G

G

E

G

Y

K

R

F

H

P

L

V

H

H

P

P

N

N

N

D

D

H

V

V

N

N

C

K

S

G

Q

Q

S

S

T

S

N

N

D

D

A

S

Y

F

P

P

T

T

A

A

A

M

R

H

L

I

A

A

M

T

V

V

E

L

A

A

T

T

A

K

P

Q

-

Q

L

L

K

I

V

P

A

A

I

L

E

N

A

N

I

L

C

L

L

T

S

Y

L

L

E

Q

V

D

K

K

G

S

R

K

E

D

F

W

S

D

H

K

I

I

L

I

K

K

M

I

G

G

R

R

T

T

Q

H

L

L

Q

Q

D

D

A

A

V

T

P

P

M

L

T

T

L

L

G

K

Q

Q

E

E

F

F

D

S

A

G

F

Y

A

I

S

T

S

Q

L

I

K

E

S

Y

D

A

I

L

G

E

R

R

I

I

D

E

D

D

A

A

C

L

K

K

E

K

L

V

C

Y

V

L

V

L

N

A

L

Q

G

G

G

G

T

T

A

A

I

V

G

G

T

T

G

G

I

I

N

N

T

S

H

K

P

I

A

G

Y

F

G

D

V

I

L

K

A

F

V

A

K

Q

A

K

L

V

A

A

D

E

I

F

T

T

G

Q

L

Q

P

P

V

F

T

K

Q

T

A

A

D

P

N

N

L

K

I

F

D

E

A

S

T

L

T

A

D

A

M

H

G

D

A

A

F

L

V

V

T

E

L

F

S

S

S

G

V

T

L

L

K

N

R

T

L

I

A

A

V

V

K

S

L

L

S

M

K

K

L

I

S

A

N

N

D

D

L

I

R

R

L

L

L

L

S

G

S

S

G

G

P

P

R

R

T

C

G

G

L

L

G

G

E

E

I

L

R

H

L

L

P

P

A

E

M

N

Q

E

P

P

G

G

S

S

S

S

I

I

M

M

P

P

G

G

K
|
K

V
|
V

N
|
N

P

P

V

T

I

Q

P

V

E

E

A

A

V

L

N

T

Q

M

V

V

A

C

F

T

Q

Q

V

V

I

M

G

G

T

N

D

H

M

V

T

T

I

V

T

T

M

I

A

A

A

G

E

S

A

N

A

G

Q

H

L

L

Q

E

L

L

N

N

A

V

M

F

E

K

P

P

V

V

I

I

V

I

Y

Y

N

N

L

I

L

L

N

Q

N

S

C

I

A

E

L

L

L

L

T

S

R

D

A

S

I

V

A

N

M

S

L

F

D

V

S

T

R

H

C

C

I

V

Q

K

G

G

I

L

E

E

A

P

N

N

E

I

A

A

K

R

C

I

E

N

Q

T

H

L

V

R

S

D

S

K

S

S

I

L

G

M

I

L

V

V

T

T

A

V

L

L

V

N

P

P

H

H

I

I

G

G

Y

Y

E

D

N

N

A

A

T

A

R

K

I

I

A

A

G

K

E

E

A

A

L

H

L

K

S

Y

G

G

A

I

G

T

V

L

A

K

E

E

L

A

V

A

R

K

R

K

D

L

G

N

L

F

L

L

S

S

D

E

D

E

A

E

L

F

S

D

Q

K

I

I

L

V

S

V

P

P

A

E

A

K

M

M

V

I

T

S

SGT 98:100 (≠ AGT 99:101) binding
KVN 324:326 (= KVN 324:326) binding

P08417 Fumarate hydratase, mitochondrial; Fumarase; EC 4.2.1.2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
41% identity, 97% coverage: 4:462/472 of query aligns to 29:487/488 of P08417

query
sites
P08417

R
|
R

I
x
T

E
|
E

H
x
T

D
|
D

L
x
A

L
x
F

G
|
G

D
x
E

A
x
I

P
x
H

V
|
V

P
|
P

A
|
A

Q
x
D

A
x
K

W

Y

Y

W

G

G

I

A

Q

Q

T

T

Q

Q

R

R

A

S

L

F

E

Q

N

N

F

F

S

K

L

I

S

G

G

G

T

A

P

R

I

E

N

R

A

M

F

P

P

L

E

P

L

L

I

V

R

H

A

A

L

F

A

G

K

V

V

L

K

K

A

K

A

S

A

A

A

A

R

I

A

V

N

N

Q

E

S

S

L

L

G

G

Q

G

L

L

S

D

D

P

I

K

K

I

A

S

N

K

A

A

I

I

A

Q

A

Q

A

A

C

A

D

D

D

E

I

V

V

A

Q

S

G

G

A

K

L

L

H

D

D

D

Q

H

F

F

V

P

V

L

D

V

L

V

I

F

Q

Q

G

T

G

G

A

S

G

G

T

T

S

Q

T

S

N

N

M

M

N

N

A

A

N

N

E

E

V

V

I

I

A

S

N

N

L

R

A

A

L

I

A

E

K

I

L

L

G

G

H

-

G

G

K

K

G

I

E

G

Y

S

R

K

H

Q

L

V

H
|
H

P

P

N

N

N

N

D

H

V

C

N

N

C

Q

S

S

Q

Q

S

S

T

S

N

N

D

D

A

T

Y

F

P

P

T

T

A

V

A

M

R

H

L

I

A

A

M

-

V

-

E

-

A

-

T

-

A

A

P

S

L

L

K

Q

V

I

A

Q

I

N

E

E

A

L

I

I

C

P

L

E

-

L

-

T

-

N

-

L

-

K

-

N

S

A

L

L

E

E

V

A

K

K

G

S

R

K

E

E

F

F

S

D

H

H

I

I

L

V

K

K

M

I

G

G

R

R

T

T

Q

H

L

L

Q

Q

D

D

A

A

V

T

P

P

M

L

T

T

L

L

G

G

Q

Q

E

E

F

F

D

S

A

G

F

Y

A

V

S

Q

S

Q

L

V

K

E

S

N

D

G

I

I

G

Q

R

R

I

V

D

A

D

H

A

S

C

L

K

K

E

T

L

L

C

S

V

F

V

L

N

A

L

Q

G

G

G

G

T

T

A

A

I

V

G

G

T

T

G

G

I

L

N

N

T

T

H

K

P

P

A

G

Y

F

G

D

V

V

L

K

A

I

V

A

K

E

A

Q

L

I

A

S

D

K

I

E

T

T

G

G

L

L

P

K

V

F

T

Q

Q

T

A

A

D

P

N

N

L

K

I

F

D

E

A

A

T

L

T

A

D

A

M

H

G

D

A

A

F

I

V

V

T

E

L

C

S

S

S

G

V

A

L

L

K

N

R

T

L

L

A

A

V

C

K

S

L

L

S

F

K

K

L

I

S

A

N

Q

D

D

L

I

R

R

L

Y

L

L

S

G

S

S

G

G

P

P

R

R

T

C

G

G

L

Y

G

H

E

E

I

L

R

M

L

L

P

P

A

E

M

N

Q

E

P

P

G

G

S

S

S

S

I

I

M

M

P

P

G

G

K

K

V

V

N

N

P

P

V

T

I

Q

P

N

E

E

A

A

V

L

N

T

Q

Q

V

V

A

C

F

V

Q

Q

V

V

I

M

G

G

T

N

D

N

M

A

T

A

I

I

T

T

M

F

A

A

A

G

E

S

A

Q

A

G

Q

Q

L

F

Q

E

L

L

N

N

A

V

M

F

E

K

P

P

V

V

I

M

V

I

Y

A

N

N

L

L

L

L

N

N

N

S

C

I

A

R

L

L

L

I

T

T

R

D

A

A

I

A

A

Y

M

S

L

F

D

R

S

V

R

H

C

C

I

V

Q

E

G

G

I

I

E

K

A

A

N

N

E

E

A

P

K

R

C

I

E

H

Q

E

H

L

V

L

S

T

S

K

S

S

I

L

G

M

I

L

V

V

T

T

A

A

L

L

V

N

P

P

H

K

I

I

G

G

Y

Y

E

D

N

A

A

A

T

S

R

K

I

V

A

A

G

K

E

N

A

A

L

H

L

K

S

K

G

G

A

I

G

T

V

L

A

K

E

E

L

S

V

A

R

L

R

E

D

L

G

G

L

V

L

L

S

T

D

E

D

K

A

E

L

F

S

D

Q

E

I

W

L

V

S

V

P

P

A

E

A

H

M

M

V

L

T

G

P

P

29:44 (vs. 4:19, 44% identical) mutation Missing: Does not affect subcellular location.
H154 (= H130) mutation to R: Abolished fumarate hydratase activity and ability to participate in DNA repair.

Sites not aligning to the query:

1:24 modified: transit peptide, Mitochondrion
24 M→S: Does not affect processing by the mitochondrial processing peptidase. Localizes both in the mitochondrion and cytosol. Exhibits high fumarate hydratase activity.; mutation M->V,I: Abolishes processing by the mitochondrial processing peptidase. Mainly localizes in the cytosol, with a small fraction in the mitochondrion. Reduced fumarate hydratase activity.
24:25 MN→SF: Does not affect processing by the mitochondrial processing peptidase. Localizes both in the mitochondrion and cytosol. Exhibits high fumarate hydratase activity.

P05042 Fumarate hydratase class II; Fumarase C; Aerobic fumarase; Iron-independent fumarase; EC 4.2.1.2 from Escherichia coli (strain K12) (see 4 papers)
41% identity, 97% coverage: 1:460/472 of query aligns to 1:460/467 of P05042

query
sites
P05042

M

M

D

N

T

T

-

V

R

R

I

S

E

E

H

K

D

D

L

S

L

M

G

G

D

A

A

I

P

D

V

V

P

P

A

A

Q

D

A

K

W

L

Y

W

G

G

I

A

Q

Q

T

T

Q

Q

R

R

A

S

L

L

E

E

N

H

F

F

S

R

L

I

S

S

G

T

T

E

P

K

I

M

N

P

A

T

F

-

P

-

E

S

L

L

I

I

R

H

A

A

L

L

A

A

K

L

V

T

K

K

A

R

A

A

A

A

A

A

R

K

A

V

N

N

Q

E

S

D

L

L

G

G

Q

L

L

L

S

S

D

E

I

E

K

K

A

A

N

S

A

A

I

I

A

R

A

Q

A

A

C

A

D

D

D

E

I

V

V

L

Q

A

G

G

A

Q

L

H

H

D

D

D

Q

E

F

F

V

P

V

L

D

A

L

I

I

W

Q

Q

G

T

G

G

A

S

G

G

T

T

S

Q

T

S

N

N

M

M

N

N

A

M

N

N

E

E

V

V

I

L

A

A

N

N

L

R

A

A

L

S

A

E

K

L

L

L

G

G

H

G

G

V

K

R

G

G

E

M

Y

E

R
|
R

H
x
K

L

V

H
|
H

P
|
P

N
|
N

N
x
D

D

D

V

V

N

N

C

K

S

S

Q

Q

S
|
S

T
x
S

N
|
N

D

D

A

V

Y

F

P

P

T

T

A

A

A

M

R

H

L

V

A

A

M

A

V

L

E

L

A

A

-

L

T

R

A

K

P

Q

L

L

K

I

V

P

A

Q

I

L

E

K

A

T

I

L

C

T

L

Q

S

T

L

L

E

N

V

E

K

K

G

S

R

R

E

A

F

F

S

A

H

D

I

I

L

V

K

K

M

I

G

G

R

R

T

T

Q
x
H

L

L

Q

Q

D

D

A

A

V

T

P

P

M

L

T

T

L

L

G

G

Q

Q

E

E

F

I

D

S

A

G

F

W

A

V

S

A

S

M

L

L

K

E

S

H

D

N

I

L

G

K

R

H

I

I

D

E

D

Y

A

S

C

L

K

P

E

H

L

V

C

A

V

E

V

L

N

A

L

L

G

G

G

G

T

T

A

A

I

V

G

G

T

T

G

G

I

L

N

N

T

T

H

H

P

P

A

E

Y

Y

G

A

V

R

L

R

A

V

V

A

K

D

A

E

L

L

A

A

D

V

I

I

T

T

G

C

L

A

P

P

V

F

T

V

Q

T

A

A

D

P

N

N

L

K

I

F

D

E

A

A

T

L

T

A

D

T

M

C

G

D

A

A

F

L

V

V

T

Q

L

A

S

H

S

G

V

A

L

L

K

K

R

G

L

L

A

A

V

A

K

S

L

L

S

M

K

K

L

I

S

A

N

N

D

D

L

V

R

R

L

W

L

L

S

A

S

S

G

G

P

P

R

R

T

C

G

G

L

I

G

G

E

E

I

I

R

S

L

I

P

P

A

E

M

N

Q
x
E

P

P

G

G

S

S

S

S

I

I

M

M

P

P

G

G

K

K

V

V

N

N

P

P

V

T

I

Q

P

C

E

E

A

A

V

L

N

T

Q

M

V

L

A

C

F

C

Q

Q

V

V

I

M

G

G

T

N

D

D

M

V

T

A

I

I

T

N

M

M

A

G

A

G

E

A

A

S

A

G

Q

N

L

F

Q

E

L

L

N

N

A

V

M

F

E

R

P

P

V

M

I

V

V

I

Y

H

N

N

L

F

L

L

N

Q

N

S

C

V

A

R

L

L

L

L

T

A

R

D

A

G

I

M

A

E

M

S

L

F

D

N

S

K

R

H

C

C

I

A

Q

V

G

G

I

I

E

E

A

P

N

N

E

R

A

E

K

R

C

I

E

N

Q

Q

H

L

V

L

S

N

S

E

S

S

I

L

G

M

I

L

V

V

T

T

A

A

L

L

V

N

P

T

H

H

I

I

G

G

Y

Y

E

D

N

K

A

A

T

A

R

E

I

I

A

A

G

K

E

K

A

A

L

H

L

K

S

E

G

G

A

L

G

T

V

L

A

K

E

A

L

A

V

A

R

L

R

A

D

L

G

G

L

Y

L

L

S

S

D

E

D

A

A

E

L

F

S

D

Q

S

I

W

L

V

S

R

P

P

A

E

A

Q

M

M

V

V

R126 (= R127) binding ; mutation to A: 10-fold decrease of fumarase activity.
K127 (≠ H128) mutation to D: No effect.
H129 (= H130) mutation to N: No effect on fumarase activity and essentially same conformation compared to the wild-type, but appears to dramatically reduce binding of ligands at the B-site.
HPND 129:132 (≠ HPNN 130:133) binding in site B
SSN 139:141 (≠ STN 140:142) binding
H188 (≠ Q188) active site, Proton donor/acceptor; mutation to N: 200-fold decrease of fumarase activity.
E315 (≠ Q315) mutation to Q: There is essentially no effect on the affinity values for both S-malate and fumarate. In contrast, the catalytic efficiency values have been lowered by 10-fold in both directions.

1fuqA Fumarase with bound 3-trimethylsilylsuccinic acid (see paper)
41% identity, 97% coverage: 4:459/472 of query aligns to 2:456/456 of 1fuqA

query
sites
1fuqA

R

R

I

S

E

E

H

K

<