SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 6936729 FitnessBrowser__SB2B:6936729 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

Q6LX42 Sodium/alanine symporter AgcS; Alanine permease; Alanine/glycine:cation symporter from Methanococcus maripaludis (strain S2 / LL) (see paper)
34% identity, 92% coverage: 4:446/480 of query aligns to 5:448/453 of Q6LX42

query
sites
Q6LX42

T

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T75 (≠ R72) binding ; binding
G79 (= G76) binding
N80 (= N77) binding ; mutation to A: Markedly reduces L-alanine uptake; when associated with A-274 and A-308.
I165 (≠ V158) mutation to A: Shows poor substrate selectivity, allowing additional amino acids such as L-valine and L-leucine to be efficiently transported. Decreases L-alanine uptake.
Q170 (= Q163) binding ; binding
F273 (= F270) mutation to A: Shows poor substrate selectivity, allowing additional amino acids such as L-valine and L-leucine to be efficiently transported. Decreases L-alanine uptake.
FS 273:274 (= FS 270:271) binding
FSNE 273:276 (= FSNE 270:273) binding
S274 (= S271) mutation to A: Markedly reduces L-alanine uptake; when associated with A-80 and A-308.
D308 (= D307) mutation to A: Markedly reduces L-alanine uptake; when associated with A-80 and A-274.
W370 (≠ N366) mutation to Q: Does not change uptake of L-alanine, but reduces the ability to transport D-alanine.

6csfM Crystal structure of sodium/alanine symporter agcs with d-alanine bound (see paper)
35% identity, 90% coverage: 14:446/480 of query aligns to 1:428/431 of 6csfM

query
sites
6csfM

W

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binding d-alanine: T58 (≠ R72), G60 (= G74), G62 (= G76), N63 (= N77), Q150 (= Q163), F253 (= F270), S254 (= S271)
binding sodium ion: T58 (≠ R72), I59 (≠ V73), N63 (= N77), S254 (= S271), D288 (= D307)

6cseM Crystal structure of sodium/alanine symporter agcs with l-alanine bound (see paper)
35% identity, 90% coverage: 14:446/480 of query aligns to 1:428/431 of 6cseM

query
sites
6cseM

W

W

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I

V

A

T

F

I

A

G

I

L

F

V

L

F

F

F

C

A

F

Y

T

S

S

T

I

V

I

L

A

G

N

W

Y

S

S

Y

Y

Y

A

G

E

E

T

K

N

C

V

F

M

E

F

Y

L

L

S

I

G

G

N

T

R

K

K

-

T

-

A

G

L

I

P

R

L

L

F

Y

R

R

L

I

L

A

V

F

L

V

G

L

M

V

V

A

M

F

F

W

G

G

A

A

M

T

A

A

K

S

I

L

S

P

L

L

V

V

W

W

D

N

L

I

A

A

D

D

V

T

S

L

M

N

G

G

L

A

M

M

A

A

I

I

V

P

N

N

I

L

I

I

A

G

L

L

V

L

L

L

L

L

S

S

G

G

L

V

A

V

I

V

R

S

V

E

I

T

D

K

D

A

Y

F

R

N

E

E

Q

I

L

R

K

K

K

N

binding alanine: A57 (= A71), T58 (≠ R72), G60 (= G74), N63 (= N77), Q150 (= Q163), F253 (= F270), S254 (= S271), E256 (= E273)
binding sodium ion: T58 (≠ R72), I59 (≠ V73), N63 (= N77), S254 (= S271), D288 (= D307)

Query Sequence

>6936729 FitnessBrowser__SB2B:6936729
MLETIVNFLNALLWGKLLVYGLVGAGLYFTVRLAFIQLTHFKHGIKVMTISRQGGDGGLS
SFQVFCTSMAARVGAGNMAGVAVAIGTAGPGAVFWMWAIAVLGMATAMIESTLAQVYKVK
DEDGQFRGGPAYYMEKGLGQRWMGALFSVFLILAFGLVFNAVQANTITGALERVFGFEPI
YVGIGIVVASGFVIMGGLRKVARVSEIVVPFMALVYLLIASVVVVVNLDKLPEVFSLIIK
SAFGWQEAVAGGVAYSVAQAMQAGIARGLFSNEAGMGSAANVAASATPNPNHPASQGFVQ
MMGVFVDTLVICTATAAIILLAGDMGAESDGIKLTIDALTSHVGDWGGAFIAFAIFLFCF
TSIIANYSYAETNVMFLSGNRKTALPLFRLLVLGMVMFGAMAKISLVWDLADVSMGLMAI
VNIIALVLLSGLAIRVIDDYREQLKKGDMPVFDRSKFPELDGQVETDIWPENAKARANEA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory