SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 6936792 FitnessBrowser__SB2B:6936792 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 17 hits to proteins with known functional sites (download)

5ey3A X-ray structure of the thymidine phosphorylase from salmonella typhimurium in complex with cytidine and sulphate
67% identity, 99% coverage: 1:438/443 of query aligns to 3:440/442 of 5ey3A

query
sites
5ey3A

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active site: D85 (= D83), K86 (= K84), H87 (= H85), S88 (= S86), T125 (= T123), D166 (= D164), R173 (= R171), S188 (= S186), K192 (= K190), K193 (= K191)
binding 4-amino-1-beta-d-ribofuranosyl-2(1h)-pyrimidinone: R117 (= R115), E130 (≠ D128)

4yyyB X-ray structure of the thymidine phosphorylase from salmonella typhimurium in complex with uridine (see paper)
67% identity, 99% coverage: 1:438/443 of query aligns to 1:438/440 of 4yyyB

query
sites
4yyyB

M

M

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active site: D83 (= D83), K84 (= K84), H85 (= H85), S86 (= S86), T123 (= T123), D164 (= D164), R171 (= R171), S186 (= S186), K190 (= K190), K191 (= K191)
binding uridine: T87 (= T87), G118 (= G118), Y168 (= Y168), R171 (= R171), S186 (= S186), I187 (= I187), K190 (= K190), S248 (= S248), Q261 (≠ D261), Y267 (= Y267)

4yekA X-ray structure of the thymidine phosphorylase from salmonella typhimurium in complex with thymidine (see paper)
67% identity, 99% coverage: 1:438/443 of query aligns to 1:438/440 of 4yekA

query
sites
4yekA

M

M

F

F

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A

G

S

D

D

A

T

L

I

D

D

Y

Y

S

S

V

V

G

G

L

F

S
x
T

N
x
D

V

M

C

A

A

R

L

L

G

G

Q

D

P

S

I

I

N

D

K

G

D

Q

T

R

P

P

L

L

A

A

V

V

I

I

H

H

A

A

Q

K

S

D

E

E

A

A

A

S

F

W

E

Q

E

E

A

A

A

A

R

K

A

A

V

V

R

K

G

A

A

A

I

I

T

I

V

L

S

D

D

D

K

K

Q

A

P

P

E

A

K

S

T

T

P

P

E

S

I

V

Y

Y

Q

R

Y

R

V

I

active site: D83 (= D83), K84 (= K84), H85 (= H85), S86 (= S86), T123 (= T123), D164 (= D164), R171 (= R171), S186 (= S186), K190 (= K190), K191 (= K191)
binding thymidine: T87 (= T87), Y168 (= Y168), R171 (= R171), S186 (= S186), F210 (= F210), S248 (= S248), Q261 (≠ D261), Y267 (= Y267), T384 (≠ S384), D385 (≠ N385)

4lhmA Thymidine phosphorylase from e.Coli with 3'-azido-3'-deoxythymidine (see paper)
67% identity, 99% coverage: 1:438/443 of query aligns to 1:438/440 of 4lhmA

query
sites
4lhmA

M

L

F

F

L

L

A

A

Q

Q

E

E

I

I

I

I

R

R

K

K

K

K

R

R

N

D

A

G

E

H

A

A

L

L

S

S

K

D

E

E

E

E

I

I

Q

R

F

F

F

F

V

I

K

N

G

G

I

I

T

R

D

D

N

N

S

T

V

I

S

S

E

E

G

G

Q

Q

I

I

A

A

A

A

L

L

G

A

M

M

A

T

V

I

Y

F

F

F

N

H

D

D

M

M

T

T

M

M

D

P

E

E

R

R

I

V

A

S

L

L

T

T

T

M

A

A

M

M

R

R

D

D

S

S

G

G

T

T

V

V

L

L

N

D

W

W

D

K

S

S

L

L

G

H

L

L

N

N

G

G

P

P

V

I

I

V

D
|
D

K
|
K

H
|
H

S
|
S

T
|
T

G

G

G

G

V

V

G

G

D

D

V

V

I

T

S
|
S

L

L

M

M

L

L

G

G

P

P

M

M

A

V

A

A

A

A

C

C

G

G

G

G

Y

Y

V

I

P

P

M

M

I

I

S
|
S

G

G

R

R

G

G

L

L

G

G

H

H

T

T

G

G

G

G

T
|
T

L

L

D

D

K

K

F

L

D

E

A

S

I

I

P

P

G

G

Y

F

Q

D

T

I

E

F

P

P

S

D

S

D

E

N

L

R

F

F

R

R

K

E

V

I

V

I

K

K

E

D

A

V

G

G

V

V

A

A

I

I

I

I

G

G

Q

Q

T

T

G

S

D

S

L

L

V

A

P

P

A

A

D
|
D

K

K

R

R

F

F

Y
|
Y

S

A

I

T

R
|
R

D

D

N

I

T

T

A
|
A

T

T

V
|
V

E
x
D

S

S

I

I

S

P

L

L

I

I

T

T

A

A

S
|
S

I

I

L

L

S

A

K
|
K

K
|
K

L

L

A

A

A

E

G

G

L

L

D

D

A

A

L

L

A

V

M

M

D

D

V

V

K

K

V

V

G

G

S

S

G

G

A

A

F
|
F

M

M

P

P

T

T

Y

Y

E

E

A

L

S

S

L

E

E

A

L

L

A

A

R

E

S

A

I

I

T

V

A

G

V

V

A

A

N

N

G

G

A

A

G

G

T

V

K
x
R

T

T

T

T

A

A

L

L

L

L

T

T

D

D

M

M

N

N

Q

Q

V

V

L

L

A

A

S

S

C

S

A

A

G

G

N

N

A

A

L

V

E

E

V

V

K
x
R

E

E

A

A

V

V

D

Q

F

F

L

L

T

T

G

G

K

E

Y

Y

R

R

N

N

P

P

R

R

L

L

Y

F

E

D

V

V

T

T

M

M

G

A

L

L

C

C

A

V

E

E

M

M

L

L

V

I

L

S

G

G

G
x
K

L

L

A

A

A

K

N

D

D

D

A

A

D

E

A

A

R

R

T

A

K

K

L

L

N

Q

T

A

V

V

L

L

D

D

N

N

G

G

R

K

A

A

A

A

E

E

I

V

F

F

G

G

K

R

M

M

V

V

S

A

G

A

L

Q

G

K

G

G

P

P

A

T

D

D

F

F

V

V

E

E

S

N

Y

Y

D

A

K

K

Y

Y

L

L

P

P

K

T

A

A

S

M

I

L

I

T

R

K

P

A

V

V

Y

Y

A

A

E

D

R

T

D

E

G

G

F

F

A

V

Y

S

S

E

M

M

V

D

T

T

R

R

E

A

L

L

G

G

L

M

A

A

V

V

V

V

T

A

L

M

G

G

G

G

G

G

R

R

R

R

K

Q

P

A

G

S

D

D

A

T

L

I

D

D

Y

Y

S

S

V

V

G

G

L

F

S

T

N

D

V

M

C

A

A

R

L

L

G

G

Q

D

P

Q

I

V

N

D

K

G

D

Q

T

R

P

P

L

L

A

A

V

V

I

I

H
|
H

A

A

Q

K

S

D

E

E

A

N

A

N

F

W

E

Q

E

E

A

A

A

A

R

K

A

A

V

V

R

K

G

A

A

A

I

I

T

K

V

L

S

A

D

D

K

K

Q

A

P

P

E

E

K

S

T

T

P

P

E

T

I

V

Y

Y

Q

R

Y
x
R

V

I

active site: D83 (= D83), K84 (= K84), H85 (= H85), S86 (= S86), T123 (= T123), D164 (= D164), R171 (= R171), S186 (= S186), K190 (= K190), K191 (= K191)
binding 3'-azido-3'-deoxythymidine: T87 (= T87), Y168 (= Y168), R171 (= R171), V177 (= V177), S186 (= S186), K190 (= K190), F210 (= F210)
binding sulfate ion: K84 (= K84), S86 (= S86), S95 (= S95), S113 (= S113), T123 (= T123), A175 (= A175), V177 (= V177), D178 (≠ E178), R234 (≠ K234), R257 (≠ K257), K288 (≠ G288), H403 (= H403), R437 (≠ Y437)

Sites not aligning to the query:

binding sulfate ion: 439

4eadA Thymidine phosphorylase from e.Coli with 3'-azido-2'-fluoro- dideoxyuridine
67% identity, 99% coverage: 1:438/443 of query aligns to 1:438/440 of 4eadA

query
sites
4eadA

M

L

F

F

L

L

A

A

Q

Q

E

E

I

I

I

I

R

R

K

K

K

K

R

R

N

D

A

G

E

H

A

A

L

L

S

S

K

D

E

E

E

E

I

I

Q

R

F

F

F

F

V

I

K

N

G

G

I

I

T

R

D

D

N

N

S

T

V

I

S

S

E

E

G

G

Q

Q

I

I

A

A

A

A

L

L

G

A

M

M

A

T

V

I

Y

F

F

F

N

H

D

D

M

M

T

T

M

M

D

P

E

E

R

R

I

V

A

S

L

L

T

T

T

M

A

A

M

M

R

R

D

D

S

S

G

G

T

T

V

V

L

L

N

D

W

W

D

K

S

S

L

L

G

H

L

L

N

N

G

G

P

P

V

I

I

V

D
|
D

K
|
K

H
|
H

S
|
S

T
|
T

G

G

G

G

V

V

G

G

D

D

V

V

I

T

S

S

L

L

M

M

L

L

G

G

P

P

M

M

A

V

A

A

A

A

C

C

G

G

G

G

Y

Y

V

I

P

P

M

M

I

I

S

S

G

G

R

R

G

G

L

L

G

G

H

H

T

T

G

G

G

G

T
|
T

L

L

D

D

K

K

F

L

D

E

A

S

I

I

P

P

G

G

Y

F

Q

D

T

I

E

F

P

P

S

D

S

D

E

N

L

R

F

F

R

R

K

E

V

I

V

I

K

K

E

D

A

V

G

G

V

V

A

A

I

I

I

I

G

G

Q

Q

T

T

G

S

D

S

L

L

V

A

P

P

A

A

D
|
D

K

K

R

R

F

F

Y
|
Y

S

A

I

T

R
|
R

D

D

N

I

T

T

A

A

T

T

V
|
V

E

D

S

S

I

I

S

P

L

L

I

I

T

T

A

A

S
|
S

I

I

L

L

S

A

K
|
K

K
|
K

L

L

A

A

A

E

G

G

L

L

D

D

A

A

L

L

A

V

M

M

D

D

V

V

K

K

V

V

G

G

S

S

G

G

A

A

F
|
F

M

M

P

P

T

T

Y

Y

E

E

A

L

S

S

L

E

E

A

L

L

A

A

R

E

S

A

I

I

T

V

A

G

V

V

A

A

N

N

G

G

A

A

G

G

T

V

K

R

T

T

T

T

A

A

L

L

L

L

T

T

D

D

M

M

N

N

Q

Q

V

V

L

L

A

A

S

S

C

S

A

A

G

G

N

N

A

A

L

V

E

E

V

V

K

R

E

E

A

A

V

V

D

Q

F

F

L

L

T

T

G

G

K

E

Y

Y

R

R

N

N

P

P

R

R

L

L

Y

F

E

D

V

V

T

T

M

M

G

A

L

L

C

C

A

V

E

E

M

M

L

L

V

I

L

S

G

G

G

K

L

L

A

A

A

K

N

D

D

D

A

A

D

E

A

A

R

R

T

A

K

K

L

L

N

Q

T

A

V

V

L

L

D

D

N

N

G

G

R

K

A

A

A

A

E

E

I

V

F

F

G

G

K

R

M

M

V

V

S

A

G

A

L

Q

G

K

G

G

P

P

A

T

D

D

F

F

V

V

E

E

S

N

Y

Y

D

A

K

K

Y

Y

L

L

P

P

K

T

A

A

S

M

I

L

I

T

R

K

P

A

V

V

Y

Y

A

A

E

D

R

T

D

E

G

G

F

F

A

V

Y

S

S

E

M

M

V

D

T

T

R

R

E

A

L

L

G

G

L

M

A

A

V

V

V

V

T

A

L

M

G

G

G

G

G

G

R

R

R

R

K

Q

P

A

G

S

D

D

A

T

L

I

D

D

Y

Y

S

S

V

V

G

G

L

F

S

T

N

D

V

M

C

A

A

R

L

L

G

G

Q

D

P

Q

I

V

N

D

K

G

D

Q

T

R

P

P

L

L

A

A

V

V

I

I

H

H

A

A

Q

K

S

D

E

E

A

N

A

N

F

W

E

Q

E

E

A

A

A

A

R

K

A

A

V

V

R

K

G

A

A

A

I

I

T

K

V

L

S

A

D

D

K

K

Q

A

P

P

E

E

K

S

T

T

P

P

E

T

I

V

Y

Y

Q

R

Y

R

V

I

active site: D83 (= D83), K84 (= K84), H85 (= H85), S86 (= S86), T123 (= T123), D164 (= D164), R171 (= R171), S186 (= S186), K190 (= K190), K191 (= K191)
binding 2',3'-dideoxy-2'-fluoro-3'-triaza-1,2-dien-2-ium-1-yluridine: T87 (= T87), Y168 (= Y168), R171 (= R171), V177 (= V177), S186 (= S186), K190 (= K190), F210 (= F210)

1brwB The crystal structure of pyrimidine nucleoside phosphorylase in a closed conformation (see paper)
43% identity, 98% coverage: 6:438/443 of query aligns to 5:432/433 of 1brwB

query
sites
1brwB

E

D

I

L

I

I

R

A

K

K

K

K

R

R

N

D

A

G

E

K

A

A

L

L

S

T

K

K

E

E

E

E

I

I

Q

E

F

W

F

I

V

V

K

R

G

G

I

Y

T

T

D

N

N

G

S

D

V

I

S

P

E

D

G

Y

Q

Q

I

M

A

S

A

A

L

L

G

A

M

M

A

A

V

I

Y

Y

F

F

N

R

D

G

M

M

T

T

M

E

D

E

E

E

R

T

I

A

A

A

L

L

T

T

T

M

A

A

M

M

R

V

D

Q

S

S

G

G

T

E

V

M

L

L

N

D

W

L

D

S

S

S

L

I

G

-

L

-

N

R

G

G

P

V

V

K

I

V

D
|
D

K
|
K

H
|
H

S
|
S

T
|
T

G

G

G

G

V

V

G
|
G

D

D

V

T

I

T

S
x
T

L

L

M

V

L

L

G

G

P

P

M

L

A

V

A

A

A

S

C

V

G

G

G

V

Y

P

V

V

P

A

M
x
K

I

M

S
|
S

G

G

R

R

G

G

L
|
L

G

G

H

H

T

T

G

G

G

G

T
|
T

L

I

D

D

K

K

F

L

D

E

A

S

I

V

P

P

G

G

Y

F

Q

H

T

V

E

E

P

I

S

S

S

K

E

D

L

E

F

F

R

I

K

R

V

L

V

V

K

N

E

E

A

N

G

G

V

I

A

A

I

I

I

I

G

G

Q

Q

T

T

G

G

D

D

L

L

V

T

P

P

A

A

D
|
D

K

K

R

K

F

L

Y
|
Y

S

A

I

L

R
|
R

D

D

N

V

T

T

A

A

T

T

V

V

E

N

S

S

I

I

S

P

L

L

I
|
I

T

A

A

S

S
|
S

I
|
I

L

M

S

S

K
|
K

K
|
K

L

I

A

A

A

A

G

G

L

A

D

D

A

A

L

I

A

V

M

L

D

D

V

V

K

K

V

T

G

G

S

A

G

G

A

A

F

F

M

M

P

K

T

K

Y

L

E

D

A

E

S

A

L

R

E

R

L

L

A

A

R

R

S

V

I

M

T

V

A

D

V

I

A

G

N

K

G

R

A

V

G

G

T

R

K

R

T

T

T

M

A

A

L

V

L

I

T

S

D

D

M

M

N

S

Q

Q

V

P

L
|
L

A

G

S

Y

C
x
A

A

V

G

G

N

N

A

A

L

L

E

E

V

V

K

K

E
|
E

A

A

V

I

D

E

F

T

L

L

T

K

G

G

K

N

Y

-

R

-

N

G

P

P

R

H

-

D

L

L

Y

T

E

E

V

L

T

C

M

L

G

T

L

L

C

G

A

S

E

H

M

M

L

V

V

Y

L

L

G

A

G

E

L

K

A

A

A

P

N

S

D

L

A

D

D

E

A

A

R

R

T

R

K

L

L

L

N

E

T

E

V

A

L

I

D

R

N

S

G

G

R

A

A

A

A

I

E

A

I

A

F

F

G

K

K

T

M

F

V

L

S

A

G

A

L

Q

G

G

G

G

P

D

A

A

D

S

F

V

V

V

E

D

S

D

Y

L

D

D

K

K

Y

-

L

L

P

P

K

K

A

A

S

A

I

Y

I

T

R

S

P

T

V

V

Y

T

A

A

E

A

R

A

D

D

G

G

F

Y

A

V

Y

A

S

E

M

M

V

A

T

A

R

D

E

D

L

I

G

G

L

T

A

A

V

A

V

M

T

W

L

L

G

G

G

A

G

G

R

R

R

A

K

K

P

K

G

E

D

D

A

V

L

I

D

D

Y

L

S

A

V

V

G

G

L

I

S

V

N

L

V

H

C

K

A

K

L

I

G

G

Q

D

P

R

I

V

N

Q

K

K

D

G

T

E

P

A

L

L

A

A

V

T

I

I

H

H

A

S

Q

N

S

R

E

P

A

D

A

V

F

L

E

D

E

V

A

K

A

E

R

K

A

-

V

I

R

E

G

A

A

A

I

I

T

R

V

L

S

S

D

P

K

Q

Q

P

P

V

E

A

K

R

T

P

P

P

E

L

I

I

Y

Y

Q

E

Y

T

V

I

active site: D80 (= D83), K81 (= K84), H82 (= H85), S83 (= S86), T120 (= T123), D161 (= D164), R168 (= R171), S183 (= S186), K187 (= K190), K188 (= K191)
binding calcium ion: G88 (= G91), L243 (= L246), A246 (≠ C249), E255 (= E258)
binding phosphate ion: H82 (= H85), S83 (= S86), T92 (≠ S95), K108 (≠ M111), S110 (= S113), T120 (= T123)
binding uracil: H82 (= H85), T84 (= T87), L114 (= L117), Y165 (= Y168), I180 (= I183), S183 (= S186), I184 (= I187)

1brwA The crystal structure of pyrimidine nucleoside phosphorylase in a closed conformation (see paper)
43% identity, 98% coverage: 6:438/443 of query aligns to 5:432/433 of 1brwA

query
sites
1brwA

E

D

I

L

I

I

R

A

K

K

K

K

R

R

N

D

A

G

E

K

A

A

L

L

S

T

K

K

E

E

E

E

I

I

Q

E

F

W

F

I

V

V

K

R

G

G

I

Y

T

T

D

N

N

G

S

D

V

I

S

P

E

D

G

Y

Q

Q

I

M

A

S

A

A

L

L

G

A

M

M

A

A

V

I

Y

Y

F

F

N

R

D

G

M

M

T

T

M

E

D

E

E

E

R

T

I

A

A

A

L

L

T

T

T

M

A

A

M

M

R

V

D

Q

S

S

G

G

T

E

V

M

L

L

N

D

W

L

D

S

S

S

L

I

G

-

L

-

N

R

G

G

P

V

V

K

I

V

D

D

K
|
K

H
|
H

S
|
S

T

T

G

G

G

G

V

V

G
|
G

D

D

V
x
T

I

T

S
x
T

L

L

M

V

L

L

G

G

P

P

M

L

A

V

A

A

A

S

C

V

G

G

G

V

Y

P

V

V

P

A

M
x
K

I

M

S

S

G

G

R

R

G

G

L

L

G

G

H

H

T

T

G

G

G

G

T

T

L

I

D

D

K

K

F

L

D

E

A

S

I

V

P

P

G

G

Y

F

Q

H

T

V

E

E

P

I

S

S

S

K

E

D

L

E

F

F

R

I

K

R

V

L

V

V

K

N

E

E

A

N

G

G

V

I

A

A

I

I

I

I

G

G

Q

Q

T

T

G

G

D

D

L

L

V

T

P

P

A

A

D
|
D

K

K

R

K

F

L

Y

Y

S

A

I

L

R
|
R

D

D

N

V

T

T

A

A

T

T

V

V

E

N

S

S

I

I

S

P

L

L

I

I

T

A

A

S

S
|
S

I

I

L

M

S

S

K
|
K

K

K

L

I

A

A

A

A

G

G

L

A

D

D

A

A

L

I

A

V

M

L

D

D

V

V

K

K

V

T

G

G

S

A

G

G

A

A

F

F

M

M

P

K

T

K

Y

L

E

D

A

E

S

A

L

R

E

R

L

L

A

A

R

R

S

V

I

M

T

V

A

D

V

I

A

G

N

K

G

R

A

V

G

G

T

R

K

R

T

T

T

M

A

A

L

V

L

I

T

S

D

D

M

M

N

S

Q

Q

V

P

L
|
L

A

G

S

Y

C
x
A

A

V

G

G

N

N

A

A

L

L

E

E

V

V

K

K

E
|
E

A

A

V

I

D

E

F

T

L

L

T

K

G

G

K

N

Y

-

R

-

N

G

P

P

R

H

-

D

L

L

Y

T

E

E

V

L

T

C

M

L

G

T

L

L

C

G

A

S

E

H

M

M

L

V

V

Y

L

L

G

A

G

E

L

K

A

A

A

P

N

S

D

L

A

D

D

E

A

A

R

R

T

R

K

L

L

L

N

E

T

E

V

A

L

I

D

R

N

S

G

G

R

A

A

A

A

I

E

A

I

A

F

F

G

K

K

T

M

F

V

L

S

A

G

A

L

Q

G

G

G

G

P

D

A

A

D

S

F

V

V

V

E

D

S

D

Y

L

D

D

K

K

Y

-

L

L

P

P

K

K

A

A

S

A

I

Y

I

T

R

S

P

T

V

V

Y

T

A

A

E

A

R

A

D

D

G

G

F

Y

A

V

Y

A

S

E

M

M

V

A

T

A

R

D

E

D

L

I

G

G

L

T

A

A

V

A

V

M

T

W

L

L

G

G

G

A

G

G

R

R

R

A

K

K

P

K

G

E

D

D

A

V

L

I

D

D

Y

L

S

A

V

V

G

G

L

I

S

V

N

L

V

H

C

K

A

K

L

I

G

G

Q

D

P

R

I

V

N

Q

K

K

D

G

T

E

P

A

L

L

A

A

V

T

I

I

H

H

A

S

Q

N

S

R

E

P

A

D

A

V

F

L

E

D

E

V

A

K

A

E

R

K

A

-

V

I

R

E

G

A

A

A

I

I

T

R

V

L

S

S

D

P

K

Q

Q

P

P

V

E

A

K

R

T

P

P

P

E

L

I

I

Y

Y

Q

E

Y

T

V

I

active site: H82 (= H85), D161 (= D164), R168 (= R171), S183 (= S186), K187 (= K190)
binding calcium ion: G88 (= G91), T90 (≠ V93), L243 (= L246), A246 (≠ C249), E255 (= E258)
binding phosphate ion: K81 (= K84), H82 (= H85), S83 (= S86), T92 (≠ S95), K108 (≠ M111)

5olnB X-ray structure of the complex pyrimidine-nucleoside phosphorylase from bacillus subtilis at 1.88 a (see paper)
44% identity, 93% coverage: 6:415/443 of query aligns to 6:411/434 of 5olnB

query
sites
5olnB

E

D

I

I

I

I

R

I

K

K

K

K

R

Q

N

N

A

G

E

K

A

E

L

L

S

T

K

T

E

E

E

E

I

I

Q

Q

F

F

F

F

V

V

K

N

G

G

I

Y

T

T

D

D

N

G

S

S

V

I

S

P

E

D

G

Y

Q

Q

I

A

A

S

A

A

L

L

G

A

M

M

A

A

V

I

Y

F

F

F

N

Q

D

D

M

M

T

S

M

D

D

R

E

E

R

R

I

A

A

D

L

L

T

T

T

M

A

A

M

M

R

V

D

N

S

S

G

G

T

E

V

T

L

I

N

D

W

L

D

S

S

A

L

I

G

-

L

-

N

E

G

G

P

I

V

K

I

V

D

D

K

K

H
|
H

S

S

T

T

G

G

G

G

V

V

G

G

D

D

V

T

I

T

S
x
T

L

L

M

V

L

L

G

A

P

P

M

L

A

V

A

A

A

A

C

L

G

D

G

V

Y

P

V

V

P

A

M

K

I

M

S
|
S

G

G

R

R

G

G

L

L

G

G

H

H

T

T

G

G

G

G

T
|
T

L

I

D

D

K

K

F

L

D

E

A

A

I

I

P

M

G

G

Y

F

Q

H

T

V

E

E

P

L

S

T

S

K

E

D

L

E

F

F

R

I

K

K

V

L

V

V

K

N

E

R

A

D

G

K

V

V

A

A

I

V

I

I

G

G

Q

Q

T

S

G

G

D

N

L

L

V

T

P

P

A

A

D
|
D

K

K

R

K

F

L

Y
|
Y

S

A

I

L

R
|
R

D

D

N

V

T

T

A

G

T

T

V

V

E

N

S

S

I

I

S

P

L

L

I
|
I

T

A

A

S

S
|
S

I

I

L

M

S

S

K
|
K

K

K

L

I

A

A

A

A

G

G

L

A

D

D

A

A

L

I

A

V

M

L

D

D

V

V

K

K

V

T

G

G

S

A

G

G

A

A

F

F

M

M

P

K

T

T

Y

E

E

E

A

D

S

A

L

A

E

E

L

L

A

A

R

K

S

A

I

M

T

V

A

R

V

I

A

G

N

N

G

N

A

V

G

G

T

R

K

Q

T

T

T

M

A

A

L

V

L

I

T

S

D

D

M

M

N

S

Q

Q

V

P

L

L

A

G

S

F

C

A

A

I

G

G

N

N

A

A

L

L

E

E

V

V

K

K

E

E

A

A

V

I

D

D

F

T

L

L

T

K

G

G

K

E

Y

-

R

-

N

G

P

P

R

E

-

D

L

L

Y

H

E

E

V

L

T

V

M

L

G

T

L

L

C

G

A

S

E

Q

M

M

L

V

V

V

L

L

G

A

G

K

L

K

A

A

A

D

N

T

D

L

A

D

D

E

A

A

R

R

T

A

K

K

L

L

N

E

T

E

V

V

L

M

D

K

N

N

G

G

R

K

A

A

A

L

E

E

I

K

F

F

G

K

K

D

M

F

V

L

S

K

G

N

L

Q

G

G

G

G

P

D

A

S

D

S

F

I

V

V

E

D

S

D

Y

P

D

S

K

K

Y

-

L

L

P

P

K

Q

A

A

S

A

I

Y

I

Q

R

I

P

D

V

V

Y

P

A

A

E

K

R

E

D

A

G

G

F

V

A

V

Y

S

S

E

M

I

V

V

T

A

R

D

E

E

L

I

G

G

L

V

A

A

V

A

V

M

T

L

L

L

G

G

G

A

G

G

R

R

R

A

K

T

P

K

G

E

D

D

A

E

L

I

D

D

Y

L

S

A

V

V

G

G

L

I

S

M

N

L

V

R

C

K

A

K

L

V

G

G

Q

D

P

K

I

V

N

E

K

K

D

G

T

E

P

P

L

L

A

V

V

T

I

L

H

Y

A

A

Q

N

S

R

E

E

A

N

A

V

F

D

E

E

E

V

A

I

A

A

R

K

active site: H83 (= H85), D162 (= D164), R169 (= R171), S184 (= S186), K188 (= K190)
binding imidazole: Y166 (= Y168), R169 (= R171), I181 (= I183), S184 (= S186)
binding sulfate ion: T93 (≠ S95), S111 (= S113), T121 (= T123)

7m7kB Crystal structure of uridine bound to geobacillus thermoglucosidasius pyrimidine nucleoside phosphorylase pynp
42% identity, 98% coverage: 6:438/443 of query aligns to 4:431/432 of 7m7kB

query
sites
7m7kB

E

D

I

L

I

I

R

A

K

K

K

K

R

R

N

D

A

G

E

Y

A

E

L

L

S

S

K

K

E

E

E

E

I

I

Q

D

F

F

F

I

V

I

K

R

G

G

I

Y

T

T

D

N

N

G

S

D

V

I

S

P

E

D

G

Y

Q

Q

I

M

A

S

A

A

L

F

G

A

M

M

A

A

V

V

Y

F

F

F

N

R

D

G

M

M

T

T

M

E

D

E

E

E

R

T

I

A

A

A

L

L

T

T

T

M

A

A

M

M

R

V

D

R

S

S

G

G

T

D

V

V

L

I

N

D

W

L

D

S

S

K

L

I

G

-

L

-

N

E

G

G

P

M

V

K

I

V

D

D

K

K

H

H

S

S

T
|
T

G

G

G

G

V

V

G

G

D

D

V

T

I

T

S

T

L

L

M

V

L

L

G

G

P

P

M

L

A

V

A

A

A

S

C

V

G

G

G

V

Y

P

V

V

P

A

M

K

I

M

S

S

G

G

R

R

G

G

L

L

G

G

H

H

T

T

G

G

G

G

T

T

L

I

D

D

K

K

F

L

D

E

A

S

I

V

P

P

G

G

Y

F

Q

H

T

V

E

E

P

I

S

D

S

N

E

E

L

Q

F

F

R

I

K

E

V

L

V

V

K

N

E

K

A

N

G

K

V

I

A

A

I

I

I

I

G

G

Q

Q

T

T

G

G

D

N

L

L

V

T

P

P

A

A

D

D

K

K

R

K

F

L

Y
|
Y

S

A

I

L

R
|
R

D

D

N

V

T

T

A

A

T

T

V

V

E

D

S

S

I

I

S

P

L

L

I

I

T

A

A

S

S
|
S

I

I

L

M

S

S

K
|
K

K

K

L

I

A

A

A

A

G

G

L

A

D

D

A

A

L

I

A

V

M

L

D

D

V

V

K

K

V

T

G

G

S

A

G

G

A

A

F
|
F

M

M

P

K

T

D

Y

F

E

A

A

G

S

A

L

K

E

R

L

L

A

A

R

T

S

A

I

M

T

V

A

E

V

I

A

G

N

K

G

R

A

V

G

G

T

R

K

K

T

T

T

M

A

A

L

V

L

I

T

S

D

D

M

M

N

S

Q

Q

V

P

L

L

A

G

S

Y

C

A

A

V

G

G

N

N

A

A

L

L

E

E

V

V

K

K

E

E

A

A

V

I

D

D

F

T

L

L

T

K

G

G

K

K

Y

-

R

-

N

G

P

P

R

E

-

D

L

L

Y

Q

E

E

V

L

T

C

M

L

G

T

L

L

C

G

A

S

E

Y

M

M

L

V

V

Y

L

L

G

A

G

E

L

K

A

A

A

S

N

S

D

L

A

E

D

E

A

A

R

R

T

A

K

L

L

L

N

E

T

A

V

S

L

I

D

R

N

E

G

G

R

K

A

A

A

L

E

E

I

T

F

F

G

K

K

V

M

F

V

L

S

S

G

A

L

Q

G

G

G

G

P

D

A

A

D

S

F

V

V

V

E

D

S

D

Y

P

D

T

K

K

Y

-

L

L

P

P

K

Q

A

A

S

K

I

Y

I

R

R

W

P

E

V

L

Y

E

A

A

E

P

R

E

D

D

G

G

F

Y

A

V

Y

A

S

E

M

I

V

V

T

A

R

D

E

E

L

V

G

G

L

T

A

A

V

A

V

M

T

L

L

L

G

G

G

A

G

G

R

R

R

A

K

T

P

K

G

E

D

A

A

T

L

I

D

D

Y

L

S

S

V

V

G

G

L

L

S

V

N

L

V

H

C

K

A

K

L

V

G

G

Q

D

P

A

I

V

N

K

K

K

D

G

T

E

P

S

L

L

A

V

V

T

I

I

H

Y

A

S

Q

N

S

T

E

E

A

-

A

N

F

I

E

E

E

E

A

V

A

K

R

Q

A

K

V

L

R

A

G

K

A

S

I

I

T

R

V

L

S

S

D

S

K

I

Q

P

P

V

E

A

K

K

T

P

P

T

E

L

I

I

Y

Y

Q

E

Y

T

V

I

binding uridine: T83 (= T87), Y164 (= Y168), R167 (= R171), S182 (= S186), K186 (= K190), F206 (= F210)

P77836 Pyrimidine-nucleoside phosphorylase; PYNP; Py-NPase; EC 2.4.2.2 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
41% identity, 91% coverage: 6:407/443 of query aligns to 5:402/433 of P77836

query
sites
P77836

E

D

I

L

I

I

R

E

K

K

K

K

R

R

N

D

A

G

E

H

A

A

L

L

S

T

K

K

E

E

E

E

I

I

Q

Q

F

F

F

I

V

I

K

E

G

G

I

Y

T

T

D

K

N

G

S

D

V

I

S

P

E

D

G

Y

Q

Q

I

M

A

S

A

A

L

L

G

A

M

M

A

A

V

I

Y

F

F

F

N

R

D

G

M

M

T

N

M

E

D

E

E

E

R

T

I

A

A

E

L

L

T

T

T

M

A

A

M

M

R

V

D

H

S

S

G

G

T

D

V

T

L

I

N

D

W

L

D

S

S

R

L

I

G

-

L

-

N

E

G

G

P

I

V

K

I

V

D

D

K
|
K

H
|
H

S
|
S

T

T

G

G

G

G

V

V

G
|
G

D

D

V
x
T

I

T

S
x
T

L

L

M

V

L

L

G

G

P

P

M

L

A

V

A

A

A

S

C

V

G

G

G

V

Y

P

V

V

P

A

M
x
K

I
x
M

S
|
S

G

G

R

R

G

G

L

L

G

G

H

H

T

T

G

G

G

G

T
|
T

L

I

D

D

K

K

F

L

D

E

A

S

I

V

P

P

G

G

Y

F

Q

H

T

V

E

E

P

I

S

T

S

N

E

D

L

E

F

F

R

I

K

D

V

L

V

V

K

N

E

K

A

N

G

K

V

I

A

A

I

V

I

V

G

G

Q

Q

T

S

G

G

D

N

L

L

V

T

P

P

A

A

D

D

K

K

R

K

F

L

Y

Y

S

A

I

L

R
|
R

D

D

N

V

T

T

A

A

T

T

V

V

E

N

S

S

I

I

S

P

L

L

I

I

T

A

A

S

S

S

I

I

L

M

S

S

K
|
K

K

K

L

I

A

A

A

A

G

G

L

A

D

D

A

A

L

I

A

V

M

L

D

D

V

V

K

K

V

T

G

G

S

V

G

G

A

A

F

F

M

M

P

K

T

D

Y

L

E

N

A

D

S

A

L

K

E

A

L

L

A

A

R

K

S

A

I

M

T

V

A

D

V

I

A

G

N

N

G

R

A

V

G

G

T

R

K

K

T

T

T

M

A

A

L

I

L

I

T

S

D

D

M

M

N

S

Q

Q

V

P

L
|
L

A

G

S

Y

C
x
A

A

I

G

G

N

N

A

A

L

L

E

E

V

V

K

K

E
|
E

A

A

V

I

D

D

F

T

L

L

T

K

G

G

K

E

Y

-

R

-

N

G

P

P

R

E

L

D

Y

F

-

Q

E

E

V

L

T

C

M

L

G

V

L

L

C

G

A

S

E

H

M

M

L

V

V

Y

L

L

G

A

G

E

L

K

A

A

A

S

N

S

D

L

A

E

D

E

A

A

R

R

T

H

K

M

L

L

N

E

T

K

V

A

L

M

D

K

N

D

G

G

R

S

A

A

A

L

E

Q

I

T

F

F

G

K

K

T

M

F

V

L

S

A

G

A

L

Q

G

G

G

G

P

D

A

A

D

S

F

V

V

V

E

D

S

D

Y

P

D

S

K

K

Y

-

L

L

P

P

K

Q

A

A

S

K

I

Y

I

I

R

I

P

E

V

L

Y

E

A

A

E

K

R

E

D

D

G

G

F

Y

A

V

Y

S

S

E

M

I

V

V

T

A

R

D

E

A

L

V

G

G

L

T

A

A

V

A

V

M

T

W

L

L

G

G

G

A

G

G

R

R

R

A

K

T

P

K

G

E

D

S

A

T

L

I

D

D

Y

L

S

A

V

V

G

G

L

L

S

V

N

L

V

R

C

K

A

K

L

V

G

G

Q

D

P

A

I

V

N

K

K

K

D

G

T

E

P

S

L

L

A

V

V

T

I

I

H

Y

A

S

Q

N

S

R

E

E

KHS 81:83 (= KHS 84:86) binding phosphate
G88 (= G91) binding K(+)
T90 (≠ V93) binding K(+)
T92 (≠ S95) binding phosphate
KMS 108:110 (≠ MIS 111:113) binding phosphate
T120 (= T123) binding phosphate
R168 (= R171) binding substrate
K187 (= K190) binding substrate
L243 (= L246) binding K(+)
A246 (≠ C249) binding K(+)
E255 (= E258) binding K(+)

2j0fA Structural basis for non-competitive product inhibition in human thymidine phosphorylase: implication for drug design (see paper)
40% identity, 96% coverage: 6:432/443 of query aligns to 5:437/446 of 2j0fA

query
sites
2j0fA

E

E

I

L

I

I

R

R

K

M

K

K

R

R

N

D

A

G

E

G

A

R

L

L

S

S

K

E

E

A

E

D

I

I

Q

R

F

G

F

F

V

V

K

A

G

A

I

V

T

V

D

N

N

G

S

S

V

A

S

Q

E

G

G

A

Q

Q

I

I

A

G

A

A

L

M

G

L

M

M

A

A

V

I

Y

R

F

L

N

R

D

G

M

M

T

D

M

L

D

E

E

E

R

T

I

S

A

V

L

L

T

T

T

Q

A

A

M

L

R

A

D

Q

S

S

G

G

T

Q

V

Q

L

L

N

E

W

W

D

P

S

E

L

-

G

A

L

W

N

R

G

Q

P

Q

V

L

I

V

D
|
D

K
|
K

H
|
H

S
|
S

T
|
T

G

G

G

G

V

V

G

G

D

D

V

K

I

V

S

S

L

L

M

V

L

L

G

A

P

P

M

A

A

L

A

A

A

A

C

C

G

G

G

C

Y

K

V

V

P

P

M

M

I

I

S

S

G

G

R

R

G

G

L
|
L

G

G

H

H

T

T

G

G

G

G

T
|
T

L

L

D

D

K

K

F

L

D

E

A

S

I

I

P

P

G

G

Y

F

Q

N

T

V

E

I

P

Q

S

S

S

P

E

E

L