SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 6936962 FitnessBrowser__SB2B:6936962 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 9 hits to proteins with known functional sites (download)

3tdtA Complex of tetrahydrodipicolinate n-succinyltransferase with 2-amino- 6-oxopimelate and coenzyme a (see paper)
79% identity, 100% coverage: 1:274/274 of query aligns to 1:274/274 of 3tdtA

query
sites
3tdtA

M

M

E

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A

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L

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Q

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M

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T

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E

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H

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K

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K

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D

D

active site: D141 (= D141), G166 (= G166), E189 (= E189)
binding 2-amino-6-oxopimelic acid: R104 (= R104), M124 (= M124), M139 (= M139), D141 (= D141)
binding coenzyme a: G185 (= G185), A186 (= A186), S203 (= S203), M204 (= M204), R217 (= R217), V234 (= V234), K254 (= K254), K259 (= K259), T260 (= T260), K263 (= K263)

2tdtA Complex of tetrahydrodipicolinate n-succinyltransferase with 2- aminopimelate and coenzyme a (see paper)
79% identity, 100% coverage: 1:274/274 of query aligns to 1:274/274 of 2tdtA

query
sites
2tdtA

M

M

E

Q

A

Q

L

L

R

Q

Q

N

R

V

I

I

E

E

A

S

A

A

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F

E

E

A

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R

R

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A

N

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T

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P

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R

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D

A

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E

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T

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L

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D

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S

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E

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K

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D

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G

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H

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W

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L

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K

A

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F

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F

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D

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C

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K

K

K

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V

D

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N

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L

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L

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D

D

active site: D141 (= D141), G166 (= G166), E189 (= E189)
binding coenzyme a: S159 (= S159), F183 (= F183), G185 (= G185), S203 (= S203), M204 (= M204), R217 (= R217), K254 (= K254), K259 (= K259), T260 (= T260), K263 (= K263)
binding (2S)-2-aminoheptanedioic acid: R104 (= R104), M124 (= M124), M139 (= M139), D141 (= D141)

1kgtA Crystal structure of tetrahydrodipicolinate n-succinyltransferase in complex with pimelate and succinyl-coa (see paper)
79% identity, 100% coverage: 1:274/274 of query aligns to 1:274/274 of 1kgtA

query
sites
1kgtA

M

M

E

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A

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L

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I

E

E

A

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F

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D

A

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E

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L

L

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D

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K

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G

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H

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W

L

L

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K

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K

A

A

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L

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F

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R

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F

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D

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N

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S

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A

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L

L

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G

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C

A

A

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I

I

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K

K

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K

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V

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N

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E

L

L

L

L

R

R

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D

D

active site: D141 (= D141), G166 (= G166), E189 (= E189)
binding pimelic acid: R104 (= R104), M124 (= M124), M139 (= M139)
binding succinyl-coenzyme a: D141 (= D141), S159 (= S159), A186 (= A186), S203 (= S203), R217 (= R217), K259 (= K259), T260 (= T260), K263 (= K263)

1kgqA Crystal structure of tetrahydrodipicolinate n-succinyltransferase in complex with l-2-aminopimelate and succinamide-coa (see paper)
79% identity, 100% coverage: 1:274/274 of query aligns to 1:274/274 of 1kgqA

query
sites
1kgqA

M

M

E

Q

A

Q

L

L

R

Q

Q

N

R

V

I

I

E

E

A

S

A

A

F

F

E

E

A

R

R

R

A

A

N

D

I

I

T

T

P

P

S

A

S

N

V

V

E

D

P

T

G

V

V

T

R

R

A

E

D

A

V

V

E

N

T

Q

V

V

I

I

N

G

M

L

L

L

D

D

K

S

G

G

E

A

M

L

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R

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V

A

A

E

E

K

K

I

I

D

D

G

G

Q

Q

W

W

H

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V

T

N

H

Q

Q

W

W

L

L

K

K

K

K

A

A

V

V

L

L

L

L

S

S

F

F

R

R

I

I

F

N

D

D

N

N

G

K

V

V

I

M

E

D

G

G

G

A

E

E

T

T

K

R

Y

Y

F

Y

D

D

K

K

V

V

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P

M

M

K

K

F

F

A

A

D

D

Y

Y

D

D

E

E

A

A

R

R

F

F

R

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A

K

E

E

A

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I

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R

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V

V

V

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P

P

P

A

A

A

T

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V

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R

K

Q

G

G

S

A

F

F

I

I

G

A

K

R

N

N

T

T

V

V

L

L

M
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P

S

S

Y

Y

V

V

N

N

L

I

G

G

A

A

Y

Y

V

V

D

D

E

E

G

G

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T

M
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M

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V

D
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D

T

T

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A

A

T

T

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V

G

G

S

S

C

C

A

A

Q

Q

I

I

G

G

K

K

N

N

V

V

H

H

L

L

S

S

G

G

G

G

V

V

G

G

I

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G

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G
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G

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V

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L

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P

L

L

Q

Q

A

A

G

N

P

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T

I

I

I

I

E

E

D

D

N

N

C

C

F
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F

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G

A

A

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E
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E

I

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V

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E

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G

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I

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E

E

E

G

G

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S

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M

G

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V

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Y

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G

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Q

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S

T

T

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R

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I

Y

Y

D

D

R
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R

E

E

T

T

G

G

E

E

I

I

H

H

Y

Y

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G

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R

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V

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P

A

A

G

G

S

S

V

V

V

V

V
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V

S

S

G

G

T

N

L

L

P

P

S

S

S

K

C

D

G

G

K

S

Y

Y

N

S

L

L

Y

Y

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C

A

A

I

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I

I

V

V

K
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K

K

K

V

V

D

D

E

A

K
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K

T
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T

R

R

G

G

K
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K

V

V

G

G

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I

N

N

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E

L

L

L

L

R

R

I

T

V

I

D

D

active site: D141 (= D141), G166 (= G166), E189 (= E189)
binding (2S)-2-aminoheptanedioic acid: R104 (= R104), M124 (= M124), M139 (= M139), D141 (= D141)
binding succinamide-coa: F183 (= F183), G185 (= G185), S203 (= S203), M204 (= M204), R217 (= R217), V234 (= V234), K254 (= K254), K259 (= K259), T260 (= T260), K263 (= K263)

3r8yA Structure of the bacillus anthracis tetrahydropicolinate succinyltransferase
42% identity, 33% coverage: 104:194/274 of query aligns to 77:168/203 of 3r8yA

query
sites
3r8yA

R

R

V

I

V

E

P

P

P

G

A

A

A

I

V

I

R

R

K

D

G

H

S

V

F

E

I

I

G

G

K

D

N

N

T

A

V

V

-

I

L

M

M

M

P

N

S

A

Y

T

V

I

N

N

L

I

G

G

A

A

Y

V

V

I

D

G

E

E

G

G

T

S

M

M

V

I

D
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D

T

M

W

N

A

A

T

V

V

L

G
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G

S
x
G

C

R

A

A

Q

T

I

V

G

G

K

K

N

N

V

C

H

H

L

V

S

G

G

A

G

G

V

A

G

V

I

L

G

A

G
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G

V

V

L

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E

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P

L

P

Q

S

A

A

G

K

P

P

T

V

I

I

I

V

E

E

D

D

N

D

C

V

F

V

I

I

G

G

A

A

R

N

S

V

E
x
V

I

V

V

L

E

E

G

G

V

V

active site: D115 (= D141), G140 (= G166), V163 (≠ E189)
binding calcium ion: G121 (= G147), G122 (≠ S148)

8bpxy Cytochrome b-c1 complex subunit Rieske-1, mitochondrial (see paper)
29% identity, 54% coverage: 83:229/274 of query aligns to 49:186/265 of 8bpxy

query
sites
8bpxy

F

F

D

D

K

K

V

S

P

P

M

L

K

-

F

-

A

-

D

-

Y

V

D

D

E

K

A

D

R

V

F

F

R

V

A

A

E

P

A

S

I

A

R

S

V

V

V

I

P

G

P

D

A

V

A

Q

V

I

R

G

K

K

G

G

S

S

F

S

I

I

G

W

K

Y

N

G

T

C

V

V

L

L

M

R

P

G

S

D

Y

V

V

N

N

N

L

I

-

S

-

V

-

G

-

S

G

G

A

T

Y

N

V

I

D

Q

E

D

G

N

T

T

M

L

V

V

D
x
H

T

V

W

A

A

K

T

T

V

N

G

I

S

S

-

G

-

K

-

V

-

L

C

P

A

T

Q

L

I

I

G

G

K

D

N

N

V

V

H

T

L

V

S

G

G

H

G

S

V

A

G

V

I

I

G
x
H

G

G

V

C

L

-

E

-

P

-

L

-

Q

-

A

-

G

-

P

-

T

-

I

T

I

V

E

E

D

D

N

D

C

A

F
|
F

I

V

G
|
G

A
x
M

R

G

S

A

E

T

I

L

V

L

E

D

G

G

V

V

V

V

V

V

E

E

E

K

G

H

S

A

V

M

I

V

S
x
A

M
x
A

G

G

V

S

Y

L

I

V

G

K

Q

Q

S

N

T

T

R

R

I

I

Y

-

D

-

R

-

E

P

T

S

G

G

E

E

I

V

H

-

Y

W

G

G

R

G

V

N

P

P

A

A

binding crotonyl coenzyme a: F144 (= F183), G146 (= G185), M147 (≠ A186), A164 (≠ S203), A165 (≠ M204)
binding zinc ion: H107 (≠ D141), H135 (≠ G165)

Sites not aligning to the query:

binding crotonyl coenzyme a: 189, 190, 203
binding : 1, 4, 5, 8, 9

8befy Cryo-em structure of the arabidopsis thaliana i+iii2 supercomplex (ci membrane core) (see paper)
29% identity, 54% coverage: 83:229/274 of query aligns to 49:186/265 of 8befy

query
sites
8befy

F

F

D

D

K

K

V

S

P

P

M

L

K

-

F

-

A

-

D

-

Y

V

D

D

E

K

A

D

R

V

F

F

R

V

A

A

E

P

A

S

I

A

R

S

V

V

V

I

P

G

P

D

A

V

A

Q

V

I

R

G

K

K

G

G

S

S

F

S

I

I

G

W

K

Y

N

G

T

C

V

V

L

L

M

R

P

G

S

D

Y

V

V

N

N

N

L

I

-

S

-

V

-

G

-

S

G

G

A

T

Y

N

V

I

D

Q

E

D

G

N

T

T

M

L

V

V

D
x
H

T

V

W

A

A

K

T

T

V

N

G

I

S

S

-

G

-

K

-

V

-

L

C

P

A

T

Q

L

I

I

G

G

K

D

N

N

V

V

H

T

L

V

S

G

G

H

G

S

V

A

G

V

I

I

G
x
H

G

G

V

C

L

-

E

-

P

-

L

-

Q

-

A

-

G

-

P

-

T

-

I

T

I

V

E

E

D

D

N

D

C

A

F
|
F

I

V

G
|
G

A
x
M

R

G

S

A

E

T

I

L

V

L

E

D

G

G

V

V

V

V

V

V

E

E

E

K

G

H

S

A

V

M

I

V

S
x
A

M
x
A

G

G

V

S

Y

L

I

V

G

K

Q

Q

S

N

T

T

R

R

I

I

Y

-

D

-

R

-

E

P

T

S

G

G

E

E

I

V

H

-

Y

W

G

G

R

G

V

N

P

P

A

A

binding Butyryl Coenzyme A: F144 (= F183), G146 (= G185), M147 (≠ A186), A164 (≠ S203), A165 (≠ M204)
binding zinc ion: H107 (≠ D141), H135 (≠ G165)

Sites not aligning to the query:

binding Butyryl Coenzyme A: 190, 203
binding : 1, 4, 5, 8, 9

7ar7y Cryo-em structure of arabidopsis thaliana complex-i (open conformation) (see paper)
29% identity, 54% coverage: 83:229/274 of query aligns to 48:185/268 of 7ar7y

query
sites
7ar7y

F

F

D

D

K

K

V

S

P

P

M

L

K

-

F

-

A

-

D

-

Y

V

D

D

E

K

A

D

R

V

F

F

R

V

A

A

E

P

A

S

I

A

R

S

V

V

V

I

P

G

P

D

A

V

A

Q

V

I

R

G

K

K

G

G

S

S

F

S

I

I

G

W

K

Y

N

G

T

C

V

V

L

L

M

R

P

G

S

D

Y

V

V

N

N

N

L

I

-

S

-

V

-

G

-

S

G

G

A

T

Y

N

V

I

D

Q

E

D

G

N

T

T

M

L

V

V

D
x
H

T

V

W

A

A

K

T

T

V

N

G

I

S

S

-

G

-

K

-

V

-

L

C

P

A

T

Q

L

I

I

G

G

K

D

N

N

V

V

H

T

L

V

S

G

G

H

G

S

V

A

G

V

I

I

G
x
H

G

G

V

C

L

-

E

-

P

-

L

-

Q

-

A

-

G

-

P

-

T

-

I

T

I

V

E

E

D

D

N

D

C

A

F

F

I

V

G

G

A

M

R

G

S

A

E

T

I

L

V

L

E

D

G

G

V

V

V

V

V

V

E

E

E

K

G

H

S

A

V

M

I

V

S

A

M

A

G

G

V

S

Y

L

I

V

G

K

Q

Q

S

N

T

T

R

R

I

I

Y

-

D

-

R

-

E

P

T

S

G

G

E

E

I

V

H

-

Y

W

G

G

R

G

V

N

P

P

A

A

binding zinc ion: H106 (≠ D141), H134 (≠ G165)

Sites not aligning to the query:

binding Phosphatidylinositol: 13, 17

7aqqy Cryo-em structure of arabidopsis thaliana complex-i (membrane core) (see paper)
29% identity, 54% coverage: 83:229/274 of query aligns to 48:185/268 of 7aqqy

query
sites
7aqqy

F

F

D

D

K

K

V

S

P

P

M

L

K

-

F

-

A

-

D

-

Y

V

D

D

E

K

A

D

R

V

F

F

R

V

A

A

E

P

A

S

I

A

R

S

V

V

V

I

P

G

P

D

A

V

A

Q

V

I

R

G

K

K

G

G

S

S

F

S

I

I

G

W

K

Y

N

G

T

C

V

V

L

L

M

R

P

G

S

D

Y

V

V

N

N

N

L

I

-

S

-

V

-

G

-

S

G

G

A

T

Y

N

V

I

D

Q

E

D

G

N

T

T

M

L

V

V

D
x
H

T

V

W

A

A

K

T

T

V

N

G

I

S

S

-

G

-

K

-

V

-

L

C

P

A

T

Q

L

I

I

G

G

K

D

N

N

V

V

H

T

L

V

S

G

G

H

G

S

V

A

G

V

I

I

G
x
H

G

G

V

C

L

-

E

-

P

-

L

-

Q

-

A

-

G

-

P

-

T

-

I

T

I

V

E

E

D

D

N

D

C

A

F

F

I

V

G

G

A

M

R

G

S

A

E

T

I

L

V

L

E

D

G

G

V

V

V

V

V

V

E

E

E

K

G

H

S

A

V

M

I

V

S

A

M

A

G

G

V

S

Y

L

I

V

G

K

Q

Q

S

N

T

T

R

R

I

I

Y

-

D

-

R

-

E

P

T

S

G

G

E

E

I

V

H

-

Y

W

G

G

R

G

V

N

P

P

A

A

binding zinc ion: H106 (≠ D141), H134 (≠ G165)

Query Sequence

>6936962 FitnessBrowser__SB2B:6936962
MEALRQRIEAAFEARANITPSSVEPGVRADVETVINMLDKGEMRVAEKIDGQWHVNQWLK
KAVLLSFRIFDNGVIEGGETKYFDKVPMKFADYDEARFRAEAIRVVPPAAVRKGSFIGKN
TVLMPSYVNLGAYVDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPLQAGPTIIED
NCFIGARSEIVEGVVVEEGSVISMGVYIGQSTRIYDRETGEIHYGRVPAGSVVVSGTLPS
SCGKYNLYAAIIVKKVDEKTRGKVGINELLRIVD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory