SitesBLAST
Comparing 6936995 FitnessBrowser__SB2B:6936995 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 14 hits to proteins with known functional sites (download)
1efpB Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
59% identity, 97% coverage: 1:242/249 of query aligns to 1:246/246 of 1efpB
- binding adenosine monophosphate: P6 (= P6), V7 (= V7), D39 (≠ C39), I63 (≠ V63), V101 (≠ I97), A119 (≠ L115), G120 (= G116), Q122 (= Q118), A123 (≠ S119), N126 (≠ G122), M128 (≠ N124), N129 (= N125), T131 (= T127)
- binding flavin-adenine dinucleotide: Y13 (≠ A13), L182 (= L178)
P38117 Electron transfer flavoprotein subunit beta; Beta-ETF from Homo sapiens (Human) (see 10 papers)
54% identity, 100% coverage: 1:248/249 of query aligns to 4:255/255 of P38117
- A9 (≠ P6) binding
- NPFC 39:42 (= NPFC 36:39) binding
- C66 (≠ V63) binding
- 123:134 (vs. 116:127, 75% identical) binding
- D128 (= D121) to N: in GA2B; decreased protein stability; dbSNP:rs104894678
- T154 (≠ V147) to M: in dbSNP:rs1130426
- R164 (= R157) to Q: in GA2B; reduced electron transfer activity; dbSNP:rs104894677
- E165 (= E158) mutation E->A,Q: Drastically increases interprotein electron transfer rates.
- 183:205 (vs. 176:198, 78% identical) Recognition loop
- L195 (= L188) mutation to A: Severely impaired in complex formation with ACADM.
- K200 (= K193) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
- KAK 200:202 (= KAK 193:195) mutation to RAR: Does not abolish methylation by ETFBKMT.
- KAKK 200:203 (≠ KAKR 193:196) mutation to RAKR: Does not abolish electron transfer activity. Abolishes sensitivity to inhibition by lysine methyltransferase ETFBKMT.
- K203 (≠ R196) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
2a1uB Crystal structure of the human etf e165betaa mutant (see paper)
54% identity, 100% coverage: 1:248/249 of query aligns to 1:252/252 of 2a1uB
- binding adenosine monophosphate: A6 (≠ P6), V7 (= V7), K8 (= K8), C39 (= C39), C63 (≠ V63), V101 (≠ I97), L119 (= L115), G120 (= G116), Q122 (= Q118), A123 (≠ S119), D126 (≠ G122), C128 (≠ N124), N129 (= N125), Q130 (= Q126), T131 (= T127)
- binding flavin-adenine dinucleotide: P37 (= P37), L182 (= L178)
Q2TBV3 Electron transfer flavoprotein subunit beta; Beta-ETF from Bos taurus (Bovine) (see paper)
54% identity, 100% coverage: 1:248/249 of query aligns to 4:255/255 of Q2TBV3
- K200 (= K193) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
- K203 (≠ R196) modified: N6,N6,N6-trimethyllysine; by ETFBKMT
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
Q9DCW4 Electron transfer flavoprotein subunit beta; Beta-ETF from Mus musculus (Mouse) (see paper)
53% identity, 100% coverage: 1:248/249 of query aligns to 4:255/255 of Q9DCW4
- K200 (= K193) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
- K203 (≠ R196) modified: N6,N6,N6-trimethyllysine; by ETFBKMT
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
P53570 Electron transfer flavoprotein subunit beta; Beta-ETF; Electron transfer flavoprotein small subunit; ETFSS from Methylophilus methylotrophus (Bacterium W3A1) (see paper)
34% identity, 84% coverage: 1:210/249 of query aligns to 1:216/264 of P53570
1o96A Structure of electron transferring flavoprotein for methylophilus methylotrophus. (see paper)
34% identity, 84% coverage: 1:210/249 of query aligns to 1:215/261 of 1o96A
- binding adenosine monophosphate: A6 (≠ P6), V7 (= V7), K8 (= K8), D38 (≠ C39), V63 (vs. gap), V99 (≠ I97), A117 (≠ L115), G118 (= G116), Q120 (= Q118), S121 (= S119), Y126 (≠ N124), A127 (≠ N125), T129 (= T127)
- binding flavin-adenine dinucleotide: Q120 (= Q118), Q182 (≠ D177)
7koeA Electron bifurcating flavoprotein fix/etfabcx (see paper)
28% identity, 97% coverage: 3:243/249 of query aligns to 4:265/282 of 7koeA
- binding flavin-adenine dinucleotide: C7 (≠ P6), I8 (≠ V7), K9 (= K8), D39 (≠ C39), M62 (≠ V63), A93 (= A88), D94 (= D89), T95 (≠ D90), T98 (≠ P93), L102 (≠ I97), T121 (≠ L115), G122 (= G116), A125 (≠ S119), G128 (= G122), T130 (≠ N124), G131 (≠ N125), V133 (≠ T127)
6fahB Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
28% identity, 84% coverage: 1:210/249 of query aligns to 1:212/262 of 6fahB
- binding flavin-adenine dinucleotide: C6 (≠ P6), A7 (≠ V7), K8 (= K8), D38 (≠ C39), T61 (≠ S62), M62 (≠ V63), A93 (= A88), D94 (= D89), T95 (≠ D90), T98 (≠ P93), L102 (≠ I97), G118 (= G116), Q120 (= Q118), G124 (= G122), T126 (≠ N124), A127 (≠ N125), V129 (≠ T127)
Sites not aligning to the query:
5ol2B The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
30% identity, 78% coverage: 1:194/249 of query aligns to 1:194/260 of 5ol2B
- binding flavin-adenine dinucleotide: C6 (≠ P6), I7 (≠ V7), K8 (= K8), D38 (≠ C39), M61 (≠ V63), A92 (= A88), D93 (= D89), T94 (≠ D90), T97 (≠ P93), A115 (≠ L115), G116 (= G116), A119 (≠ S119), G122 (= G122), T124 (≠ N124), A125 (≠ N125), V127 (≠ T127)
4l2iB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
30% identity, 85% coverage: 1:211/249 of query aligns to 1:211/263 of 4l2iB
- binding flavin-adenine dinucleotide: C6 (≠ P6), V7 (= V7), K8 (= K8), Y37 (≠ F38), D38 (≠ C39), Y40 (≠ I41), T60 (≠ S62), M61 (≠ V63), A92 (= A88), D93 (= D89), T94 (≠ D90), T97 (≠ P93), M101 (≠ I97), C116 (≠ L115), G117 (= G116), Q119 (= Q118), A120 (≠ S119), G123 (= G122), T125 (≠ N124), A126 (≠ N125), V128 (≠ T127)
- binding nicotinamide-adenine-dinucleotide: M61 (≠ V63), G62 (= G64), P63 (≠ S65), S85 (≠ E87), D86 (vs. gap), G91 (vs. gap)
4kpuB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
30% identity, 85% coverage: 1:211/249 of query aligns to 1:211/263 of 4kpuB
- binding flavin-adenine dinucleotide: C6 (≠ P6), V7 (= V7), K8 (= K8), Y37 (≠ F38), D38 (≠ C39), Y40 (≠ I41), M61 (≠ V63), A92 (= A88), D93 (= D89), T94 (≠ D90), T97 (≠ P93), C116 (≠ L115), G117 (= G116), Q119 (= Q118), A120 (≠ S119), G123 (= G122), T125 (≠ N124), A126 (≠ N125), V128 (≠ T127)
5ow0B Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
31% identity, 76% coverage: 35:223/249 of query aligns to 35:220/251 of 5ow0B
- binding adenosine monophosphate: D39 (≠ C39), Y62 (≠ V63), I95 (≠ S96), A114 (≠ L115), G115 (= G116), Q117 (= Q118), S118 (= S119), R123 (≠ N124), G124 (≠ N125), V125 (≠ Q126), V126 (≠ T127), P127 (≠ G128)
- binding flavin-adenine dinucleotide: L37 (≠ P37), Y38 (≠ F38)
Sites not aligning to the query:
7qh2B Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
28% identity, 77% coverage: 2:193/249 of query aligns to 3:199/265 of 7qh2B
- binding flavin-adenine dinucleotide: C7 (≠ P6), K9 (= K8), D39 (≠ C39), M62 (≠ V63), D94 (= D89), V95 (≠ D90), L102 (≠ I97), G118 (= G116), Q120 (= Q118), T121 (≠ S119), T126 (≠ N124), A127 (≠ N125), V129 (≠ T127)
Query Sequence
>6936995 FitnessBrowser__SB2B:6936995
MKILVPVKRVVDANVKVRVKADNTDVDTANLKMAINPFCEIAVEEAVRLKEAGHASEIVV
VSVGSKAVQEQMRTAMALGADRGIHVEADDNLPAISIAKILKAVQDKEQAQLVLLGKQSI
DGDNNQTGQMLAALMDAPQATFASKVVVEGGELLVTREVDGGLQTLKLPMPAIVTSDLRL
NEPRYAKLPDIMKAKRKPLDTLSPADLGVTLKQHHKLLSVAAPATRQAGIKVSSVEELVE
KLKNEAKVI
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory