SitesBLAST
Comparing 6936996 FitnessBrowser__SB2B:6936996 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 11 hits to proteins with known functional sites (download)
P13804 Electron transfer flavoprotein subunit alpha, mitochondrial; Alpha-ETF from Homo sapiens (Human) (see 6 papers)
59% identity, 100% coverage: 1:307/307 of query aligns to 20:330/333 of P13804
- 20:204 (vs. 1:181, 45% identical) Domain I
- G116 (= G95) to R: in GA2A; impaired protein stability and loss of electron transfer activity; dbSNP:rs119458971
- T171 (≠ S150) to I: decreased protein stability; dbSNP:rs1801591
- R223 (= R200) binding FAD
- S248 (= S225) binding FAD
- R249 (= R226) mutation to A: Loss of electron transfer activity.
- VGQT 263:266 (= VGQT 240:243) binding FAD
- T266 (= T243) to M: in GA2A; decreased electron transfer activity; dbSNP:rs119458970
- SGAIQH 281:286 (= SGAIQH 258:263) binding FAD
- N300 (= N277) binding FAD
- DL 318:319 (= DL 295:296) binding FAD
Sites not aligning to the query:
2a1uA Crystal structure of the human etf e165betaa mutant (see paper)
59% identity, 100% coverage: 1:307/307 of query aligns to 2:312/315 of 2a1uA
- binding flavin-adenine dinucleotide: G204 (= G199), R205 (= R200), S230 (= S225), R231 (= R226), A232 (= A227), Q244 (= Q239), V245 (= V240), G246 (= G241), T248 (= T243), G261 (= G256), I262 (= I257), S263 (= S258), A265 (= A260), Q267 (= Q262), H268 (= H263), N282 (= N277), K283 (= K278), D300 (= D295), L301 (= L296)
1efpA Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
59% identity, 98% coverage: 2:303/307 of query aligns to 1:303/307 of 1efpA
- binding flavin-adenine dinucleotide: G199 (= G199), R200 (= R200), G201 (= G201), S225 (= S225), R226 (= R226), A227 (= A227), Q239 (= Q239), V240 (= V240), G241 (= G241), T243 (= T243), G256 (= G256), I257 (= I257), S258 (= S258), A260 (= A260), Q262 (= Q262), H263 (= H263), N277 (= N277), K278 (= K278), D295 (= D295), L296 (= L296)
6fahE Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
38% identity, 92% coverage: 25:307/307 of query aligns to 94:388/393 of 6fahE
- binding flavin-adenine dinucleotide: L180 (≠ Q110), R200 (= R125), M281 (≠ R200), G282 (= G201), R307 (= R226), A308 (= A227), Q320 (= Q239), V321 (= V240), G322 (= G241), Q323 (= Q242), T324 (= T243), G337 (= G256), I338 (= I257), S339 (= S258), Q343 (= Q262), H344 (= H263), N358 (= N277), K359 (= K278), L377 (= L296)
Sites not aligning to the query:
- binding iron/sulfur cluster: 7, 10, 13, 17, 18, 35, 36, 37, 38, 41, 45, 49
4kpuA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
37% identity, 80% coverage: 57:303/307 of query aligns to 70:328/338 of 4kpuA
- binding flavin-adenine dinucleotide: L125 (≠ Q110), R144 (= R125), I155 (≠ V136), G224 (= G199), R225 (= R200), G226 (= G201), S250 (= S225), R251 (= R226), A252 (= A227), Q264 (= Q239), V265 (= V240), G266 (= G241), Q267 (= Q242), S268 (≠ T243), G281 (= G256), I282 (= I257), S283 (= S258), S285 (≠ A260), Q287 (= Q262), H288 (= H263), N302 (= N277), K303 (= K278), D320 (= D295), A321 (≠ L296)
5ol2A The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
36% identity, 99% coverage: 3:307/307 of query aligns to 5:324/331 of 5ol2A
- binding calcium ion: E75 (= E71), D188 (≠ S174)
- binding flavin-adenine dinucleotide: T117 (≠ L111), R136 (= R125), I147 (≠ V136), G216 (= G199), R217 (= R200), G218 (= G201), S242 (= S225), R243 (= R226), A244 (= A227), Q256 (= Q239), V257 (= V240), G258 (= G241), T260 (= T243), G273 (= G256), I274 (= I257), S275 (= S258), A277 (= A260), Q279 (= Q262), H280 (= H263), N294 (= N277), K295 (= K278), D312 (= D295), V313 (≠ L296)
5ow0A Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
41% identity, 78% coverage: 69:307/307 of query aligns to 52:291/292 of 5ow0A
- binding flavin-adenine dinucleotide: G183 (= G199), R184 (= R200), G185 (= G201), S209 (= S225), R210 (= R226), Q223 (= Q239), I224 (≠ V240), G225 (= G241), T227 (= T243), G240 (= G256), V241 (≠ I257), S242 (= S258), A244 (= A260), Q246 (= Q262), H247 (= H263), N261 (= N277), K262 (= K278), D279 (= D295), Y280 (≠ L296)
7qh2A Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
32% identity, 99% coverage: 3:307/307 of query aligns to 13:331/337 of 7qh2A
- binding flavin-adenine dinucleotide: L125 (vs. gap), T126 (vs. gap), R144 (= R125), I155 (≠ V136), R224 (= R200), G225 (= G201), T249 (≠ S225), R250 (= R226), Q263 (= Q239), I264 (≠ V240), G265 (= G241), L266 (≠ Q242), S267 (≠ T243), G280 (= G256), I281 (= I257), S282 (= S258), Q286 (= Q262), N301 (= N277), S302 (≠ K278), D303 (= D279), D319 (= D295), L320 (= L296)
7koeB Electron bifurcating flavoprotein fix/etfabcx (see paper)
34% identity, 98% coverage: 3:303/307 of query aligns to 7:323/336 of 7koeB
- binding flavin-adenine dinucleotide: T121 (≠ L111), R140 (= R125), T142 (≠ I127), G219 (= G199), K220 (≠ R200), G221 (= G201), S245 (= S225), R246 (= R226), A247 (= A227), Q259 (= Q239), V260 (= V240), G261 (= G241), Q262 (= Q242), T263 (= T243), G276 (= G256), S278 (= S258), Q282 (= Q262), H283 (= H263), N297 (= N277), I298 (≠ K278), L316 (= L296)
P53571 Electron transfer flavoprotein subunit alpha; Alpha-ETF; Electron transfer flavoprotein large subunit; ETFLS from Methylophilus methylotrophus (Bacterium W3A1) (see 2 papers)
39% identity, 50% coverage: 144:298/307 of query aligns to 153:310/321 of P53571
- R211 (= R200) binding FAD
- SR 236:237 (= SR 225:226) binding FAD
- QVGQS 250:254 (≠ QVGQT 239:243) binding FAD
- 268:275 (vs. 256:263, 88% identical) binding FAD
- N289 (= N277) binding FAD
- DI 307:308 (≠ DL 295:296) binding FAD
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
3clrD Crystal structure of the r236a etf mutant from m. Methylotrophus (see paper)
39% identity, 50% coverage: 144:298/307 of query aligns to 152:309/319 of 3clrD
- binding flavin-adenine dinucleotide: G209 (= G199), R210 (= R200), G211 (= G201), S235 (= S225), A236 (≠ R226), P237 (≠ A227), Q249 (= Q239), V250 (= V240), G251 (= G241), Q252 (= Q242), S253 (≠ T243), G267 (= G256), I268 (= I257), S269 (= S258), S271 (≠ A260), Q273 (= Q262), H274 (= H263), N288 (= N277), T289 (≠ K278), D306 (= D295), I307 (≠ L296)
Query Sequence
>6936996 FitnessBrowser__SB2B:6936996
MSILVLAEHDNASLKLDTAKVVSAAKAIGGEVHLLVAGHNCGAVADAAAAIDGVAKVLVA
DNAAYAAHLGENLAALMLDLAGNYSHILAAASSMGKDALPRVAALLDVAQLSEVVKVVDA
NTFVRPIYAGNAMATVESLDDKKVMTVRPSAFDAAANGGSAAIEALDKVFTAKSAFVSQE
LTVSERPELGNAGIIVSGGRGMGSGENFTLLEKLADKLGAAVGASRAAVDAGFVPNDLQV
GQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADYALEADLFEAV
PKLIDLL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory