SitesBLAST

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
29% identity, 38% coverage: 76:178/273 of query aligns to 78:190/203 of P07464

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P07464

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N85 (≠ D83) binding in other chain; binding in other chain
D93 (≠ G91) binding
H115 (= H119) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ A130) binding in other chain
A160 (≠ S148) binding in other chain
TK 165:166 (≠ VK 153:154) binding
R180 (= R168) binding
R183 (≠ A171) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding
71 binding

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
29% identity, 38% coverage: 76:178/273 of query aligns to 77:189/201 of 1krvA

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1krvA

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binding 4-nitrophenyl beta-D-galactopyranoside: Y82 (≠ F81), N84 (≠ D83), V90 (= V89), D92 (≠ G91), M126 (vs. gap)
binding coenzyme a: A104 (≠ Y103), S110 (≠ G109), T112 (= T111), W138 (≠ V127), G140 (= G129), S141 (≠ A130), N146 (≠ L135), A159 (≠ S148), P177 (= P166), R179 (= R168), R182 (≠ A171)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16, 25, 70

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
29% identity, 38% coverage: 76:178/273 of query aligns to 77:189/201 of 1kruA

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binding coenzyme a: H114 (= H119), W138 (≠ V127), G140 (= G129), S141 (≠ A130), G158 (= G147), A159 (≠ S148), P177 (= P166), R182 (≠ A171)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: Y82 (≠ F81), Y82 (≠ F81), N84 (≠ D83), V90 (= V89), D92 (≠ G91), L102 (≠ T101), H114 (= H119), M126 (vs. gap)

Sites not aligning to the query:

binding 1-methylethyl 1-thio-beta-D-galactopyranoside: 16, 22, 25, 62, 70

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
29% identity, 38% coverage: 76:178/273 of query aligns to 77:189/200 of 1krrA

query
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1krrA

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binding acetyl coenzyme *a: N84 (≠ D83), I103 (≠ L102), A104 (≠ Y103), P105 (≠ H104), T112 (= T111), H114 (= H119), G140 (= G129), S141 (≠ A130), N146 (≠ L135), G158 (= G147), A159 (≠ S148), A174 (≠ V163), P177 (= P166), R182 (≠ A171)

Query Sequence

>6937121 FitnessBrowser__SB2B:6937121
MGVISRLKEDIASIYHRDPAARSAIEILLNYPGMHAIWLHRISHKLWKRDWRFLARCLST
FSRWLTGVEIHPGATIGRRFFIDHGMGIVIGETAEIGDDCTLYHGVTLGGTSWNAGKRHP
TLENNVVVGAGAKILGPITMKEGSRVGSNSVVVKDVPEHTTVVGIPGRAVAQPSSQSKET
TERRSAMAKKYGFDAYAVSPDNPDPVANAIGQMLDHMHLMDSKVQDLCQELQRLGGNVCT
ERLPELDVGEFSEAEAAAAEKRQNALDEFDPSI

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SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory