SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 6937149 FitnessBrowser__SB2B:6937149 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
45% identity, 92% coverage: 14:416/437 of query aligns to 15:423/425 of O59010

query
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O59010

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S65 (= S70) mutation to V: Strongly decreased chloride conductance.
R276 (≠ A274) mutation to S: Increased rate of aspartate transport; when associated with R-395.
RSS 276:278 (≠ ASS 274:276) binding
M311 (= M310) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
T314 (= T313) binding
V355 (= V354) binding
D394 (= D387) binding
M395 (= M388) mutation to R: Increased rate of aspartate transport; when associated with S-276.
R397 (= R390) mutation to A: Strongly decreased affinity for aspartate.
N401 (= N394) binding
D405 (= D398) mutation to N: Strongly decreased affinity for aspartate.

6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
45% identity, 91% coverage: 14:410/437 of query aligns to 7:409/409 of 6bavA

query
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6bavA

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binding benzylcysteine: R268 (≠ A274), S270 (= S276), T306 (= T313), Y309 (≠ M316), D382 (= D383), D386 (= D387), C389 (≠ R390), T390 (= T391)

6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
45% identity, 91% coverage: 14:409/437 of query aligns to 7:408/408 of 6bauA

query
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6bauA

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binding cysteine: S270 (= S276), M303 (= M310), T306 (= T313), A345 (= A352), G346 (= G353), V347 (= V354), G351 (= G358), D386 (= D387), C389 (≠ R390), T390 (= T391), N393 (= N394)

6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
44% identity, 91% coverage: 14:412/437 of query aligns to 15:419/419 of 6x15A

query
sites
6x15A

K

K

I

I

L

L

I

I

G

G

M

L

G

I

A

L

G

G

I

A

L

I

I

V

G

G

L

L

L

I

L

L

R

G

N

H

F

Y

F

-

G

G

G

Y

S

A

E

H

W

A

V

V

Q

H

D

T

Y

Y

I

V

T

K

E

P

G

-

F

-

H

-

V

-

I
x
F

G

G

T

D

I

L

F

F

I

V

N

R

S

L

L

L

K

C

M

M

L

L

V

V

V

M

P

P

L

I

V

V

F

F

I

A

S

S

L

L

V

V

C

V

G

G

T

A

C

A

S

S

L

I

S

S

E

-

P
|
P

S

A

K

R

L

L

G

G

R

R

L

V

G

G

G

V

K

K

T

I

L

V

A

V

F

Y

Y
|
Y

L

L

F
x
L

T
|
T

T
x
S

A

A

I
x
F

A

A

L

V

V

T

V

L

A

G

I

I

S

I

A

M

A

A

V

R

L

L

V

F

Q

N

P

P

G

G

N

A

A

G

-

I

S

H

L

L

A

A

S

V

E

G

S

G

M

Q

Q

Q

Y

F

S

Q

A

P

K

H

E

Q

A

A

P

P

S

P

L
|
L

A

V

D

H

V
x
I

L

L

I

L

N

D

I

I

V

V

P

P

S

T

N

N

P

P

M

F

K

G

A

A

L

L

S

A

E

N

G

G

N

Q

M

V

L

L

Q

P

I

T

I

I

I

F

F

F

A

A

V
x
I

I

I

F

L

G

G

F

I

A

A

I

I

S

T

H
x
Y

I

L

-

M

-

N

-

S

-

E

G

N

E

E

R

K

G

V

R

R

R

K

V

S

A

A

-

E

A

T

L

L

F

L

D

D

D

A

L

I

N

N

-

G

-

L

-

A

E

E

V

A

I

M

M

Y

R
x
K

V

I

V

V

T

N

L
x
G

I

V

M

M

Q

Q

L

Y

A

A

P

P

Y
x
I

G

G

V

V

F
|
F

A

A

L

L

M

I

G

A

K

Y

L

V

A

M

L

A

T

E

L

Q

G

G

M

V

E

H

T

V

L

V

E

G

S

E

V

L

I

A

K

K

Y

V

F

T

M

A

L

A

V

V

L

Y

V

V

V

G

L

L

L

T

F

L

H

Q

G

I

F

L

V

L

V

V

Y

Y

P

F

T

V

L
|
L

L

L

K

K

L

I

F

Y

S

-

G

G

L

I

S

D

P

P

L

I

M

S

F

F

I
|
I

R

K

K

H

M

A

R

K

D

D

V

A

Q
x
M

L

L

F

T

A

A

F

F

S

V

T

T

A

R

S
|
S

N

S

A

G

T

T

L

L

P

P

V

V

T

T

M

M

E

R

A

V

S

A

E

K

H

-

R

E

L

M

G

G

A

I

D

S

N

E

K

G

V

I

A

Y

S

S

F

F

T

T

L

L

P

P

L

L

G
|
G

A

A

T
|
T

I

I

N
|
N

M
|
M

D
|
D

G

G

T
|
T

A

A

I

L

M

Y

Q

Q

G

G

V

V

A

A

T

T

V

F

F

F

I

I

A

A

Q

N

V

A

F

L

G

G

I

S

D

H

L

L

T
|
T

I
x
V

T
x
G

D

Q

Y

Q

A

L

M
x
T

V

I

V

V

M
x
L

T

T

A

A

T

V

L

L

A

A

S
|
S

I
|
I

G

G

T
|
T

A

A

G
|
G

V

V

P

P

G

G

V
x
A

G
|
G

L

A

V

I

M

M

L

L

A

C

M

M

V

V

L

L

N

H

Q

S

V

V

G

G

L

L

P

P

V

L

E

T

-

D

-

P

-

N

-

V

-

A

G

A

I

Y

A

A

L

M

I

I

L

L

G

G

V

I

D

D

R

A

M

I

L

L

D
|
D

M

M

V

G

R
|
R

T
|
T

A
x
M

V

V

N
|
N

V
|
V

T

T

G

G

D
|
D

T

L

V

T

A

G

T

T

V

A

V

I

I

V

A

A

K

K

S

T

E

E

G

G

A

T

L

L

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: F46 (≠ I51), F46 (≠ I51), P75 (= P81), L91 (≠ F97), F95 (≠ I101), L130 (= L135), I133 (≠ V138), I159 (≠ V164), Y167 (≠ H172), K196 (≠ R193), G200 (≠ L197), I207 (≠ Y204), F210 (= F207), L250 (= L247), I262 (= I260), M269 (≠ Q267), T334 (= T333), V335 (≠ I334), G336 (≠ T335), T340 (≠ M339), L343 (≠ M342), M399 (≠ A392)
binding aspartic acid: S277 (= S275), S278 (= S276), T314 (= T313), G354 (= G353), A358 (≠ V357), G359 (= G358), D394 (= D387), R397 (= R390), T398 (= T391)
binding sodium ion: Y89 (= Y95), T92 (= T98), S93 (≠ T99), G306 (= G305), T308 (= T307), N310 (= N309), N310 (= N309), M311 (= M310), D312 (= D311), S349 (= S348), I350 (= I349), T352 (= T351), N401 (= N394), V402 (= V395), D405 (= D398)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 4, 7

2nwwA Crystal structure of gltph in complex with tboa (see paper)
45% identity, 91% coverage: 14:409/437 of query aligns to 6:407/407 of 2nwwA

query
sites
2nwwA

K

K

I

I

L

L

I

I

G

G

M

L

G

I

A

L

G

G

I

A

L

I

I

V

G

G

L

L

L

I

L

L

R

G

N

H

F

Y

F

-

G

G

G

Y

S

A

E

H

W

A

V

V

Q

H

D

T

Y

Y

I

V

T

K

E

P

G

-

F

-

H

-

V

-

I

F

G

G

T

D

I

L

F

F

I

V

N

R

S

L

L

L

K

K

M

M

L

L

V

V

V

M

P

P

L

I

V

V

F

F

I

A

S

S

L

L

V

V

C

V

G

G

T

A

C

A

S

S

L

I

S

S

E

-

P

P

S

A

K

R

L

L

G

G

R

R

L

V

G

G

G

V

K

K

T

I

L

V

A

V

F

Y

Y

Y

L

L

F

L

T

T

T

S

A

A

I

F

A

A

L

V

V

T

V

L

A

G

I

I

S

I

A

M

A

A

V

R

L

L

V

F

Q

N

P

P

G

G

N

A

A

G

-

I

S

H

L

L

A

A

S

V

E

G

S

G

M

Q

Q

Q

Y

F

S

Q

A

P

K

H

E

Q

A

A

P

P

S

P

L

L

A

V

D

H

V

I

L

L

I

L

N

D

I

I

V

V

P

P

S

T

N

N

P

P

M

F

K

G

A

A

L

L

S

A

E

N

G

G

N

Q

M

V

L

L

Q

P

I

T

I

I

I

F

F

F

A

A

V

I

I

I

F

L

G

G

F

I

A

A

I

I

S

T

H

Y

I

L

-

M

-

N

-

S

-

E

G

N

E

E

R

K

G

V

R

R

R

K

V

S

A

A

-

E

A

T

L

L

F

L

D

D

D

A

L

I

N

N

-

G

-

L

-

A

E

E

V

A

I

M

M

Y

R

K

V

I

V

V

T

N

L

G

I

V

M

M

Q

Q

L

Y

A

A

P

P

Y

I

G

G

V

V

F

F

A

A

L

L

M

I

G

A

K

Y

L

V

A

M

L

A

T

E

L

Q

G

G

M

V

E

H

T

V

L

V

E

G

S

E

V

L

I

A

K

K

Y

V

F

T

M

A

L

A

V

V

L

Y

V

V

V

G

L

L

L

T

F

L

H

Q

G

I

F

L

V

L

V

V

Y

Y

P

F

T

V

L

L

K

K

L

I

F

Y

S

-

G

G

L

I

S

D

P

P

L

I

M

S

F

F

I

I

R

K

K

H

M

A

R

K

D

D

V

A

Q

M

L

L

F

T

A

A

F

F

S

V

T

T

A

R

S

S

S
|
S

N

S

A

G

T

T

L

L

P

P

V

V

T

T

M

M

E

R

A

V

S

A

E

K

H

-

R

E

L

M

G

G

A

I

D

S

N

E

K

G

V

I

A

Y

S

S

F

F

T

T

L

L

P

P

L

L

G

G

A

A

T

T

I

I

N

N

M

M

D

D

G

G

T
|
T

A

A

I

L

M

Y

Q

Q

G

G

V

V

A

C

T

T

V

F

F

F

I

I

A

A

Q

N

V

A

F

L

G

G

I

S

D

H

L

L

T

T

I

V

T

G

D

Q

Y

Q

A

L

M

T

V

I

V

V

M

L

T

T

A

A

T

V

L

L

A

A

S

S

I

I

G

G

T

T

A

A

G

G

V

V

P

P

G

G

V
x
A

G
|
G

L

A

V

I

M

M

L

L

A

A

M

M

V

V

L

L

N

H

Q

S

V

V

G

G

L

L

P

P

V

L

E

T

-

D

-

P

-

N

-

V

-

A

G

A

I

Y

A

A

L

M

I

I

L

L

G

G

V

I

D

D

R

A

M

I

L

L

D
|
D

M

M

V

G

R
|
R

T
|
T

A

M

V

V

N
|
N

V

V

T

T

G

G

D

D

T

L

V

T

A

G

T

T

V

A

V

I

I

V

A

A

K

K

S

T

E

E

binding (3s)-3-(benzyloxy)-l-aspartic acid: S269 (= S276), T305 (= T313), A349 (≠ V357), G350 (= G358), D385 (= D387), R388 (= R390), T389 (= T391), N392 (= N394)

6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
44% identity, 91% coverage: 14:409/437 of query aligns to 12:413/413 of 6x14A

query
sites
6x14A

K

K

I
|
I

L

L

I

I

G

G

M

L

G

I

A

L

G

G

I

A

L

I

I

V

G

G

L

L

L

I

L

L

R

G

N

H

F

Y

F

-

G

G

G

Y

S

A

E

H

W

A

V

V

Q

H

D

T

Y

Y

I

V

T

K

E

P

G

-

F

-

H

-

V

-

I

F

G

G

T

D

I

L

F

F

I

V

N

R

S

L

L

L

K

C

M

M

L

L

V

V

V

M

P

P

L

I

V

V

F

F

I

A

S

S

L

L

V

V

C

V

G
|
G

T

A

C

A

S

S

L

I

S

S

E

-

P

P

S

A

K

R

L

L

G

G

R

R

L

V

G

G

G

V

K

K

T

I

L
x
V

A

V

F

Y

Y

Y

L

L

F

L

T

T

T

S

A

A

I

F

A

A

L

V

V

T

V

L

A

G

I

I

S

I

A

M

A

A

V

R

L

L

V

F

Q

N

P

P

G

G

N

A

A

G

-

I

S

H

L

L

A

A

S

V

E

G

S

G

M

Q

Q

Q

Y

F

S

Q

A

P

K

H

E

Q

A

A

P

P

S

P

L

L

A

V

D

H

V

I

L

L

I

L

N

D

I

I

V

V

P

P

S

T

N

N

P

P

M

F

K

G

A

A

L

L

S

A

E

N

G

G

N

Q

M

V

L

L

Q

P

I

T

I

I

I

F

F

F

A

A

V

I

I
|
I

F

L

G

G

F

I

A

A

I

I

S

T

H
x
Y

I

L

-

M

-

N

-

S

-

E

G

N

E

E

R

K

G

V

R

R

R

K

V

S

A

A

-

E

A

T

L

L

F

L

D

D

D

A

L

I

N

N

-

G

-

L

-

A

E

E

V

A

I

M

M

Y

R
x
K

V

I

V

V

T

N

L

G

I

V

M
|
M

Q

Q

L

Y

A

A

P

P

Y

I

G

G

V

V

F

F

A

A

L

L

M

I

G

A

K

Y

L

V

A

M

L

A

T

E

L

Q

G

G

M

V

E

H

T

V

L

V

E

G

S

E

V

L

I

A

K

K

Y

V

F

T

M

A

L

A

V

V

L

Y

V

V

V

G

L

L

L

T

F

L

H

Q

G

I

F

L

V

L

V

V

Y

Y

P

F

T

V

L

L

K

K

L

I

F

Y

S

-

G

G

L

I

S

D

P

P

L

I

M

S

F

F

I

I

R

K

K

H

M

A

R

K

D

D

V

A

Q

M

L

L

F

T

A

A

F

F

S

V

T

T

A

R

S

S

S
|
S

N

S

A

G

T

T

L

L

P

P

V

V

T

T

M

M

E

R

A

V

S

A

E

K

H

-

R

E

L

M

G

G

A

I

D

S

N

E

K

G

V

I

A

Y

S

S

F

F

T

T

L

L

P

P

L

L

G

G

A

A

T
|
T

I
|
I

N

N

M

M

D

D

G

G

T
|
T

A

A

I

L

M

Y

Q

Q

G

G

V

V

A

A

T

T

V

F

F

F

I

I

A

A

Q

N

V

A

F

L

G

G

I

S

D

H

L

L

T

T

I

V

T

G

D

Q

Y

Q

A

L

M

T

V

I

V

V

M

L

T

T

A

A

T

V

L

L

A

A

S

S

I
|
I

G

G

T

T

A

A

G

G

V

V

P

P

G

G

V

A

G
|
G

L

A

V

I

M

M

L

L

A

C

M

M

V

V

L

L

N

H

Q

S

V

V

G

G

L

L

P

P

V

L

E

T

-

D

-

P

-

N

-

V

-

A

G

A

I

Y

A

A

L

M

I

I

L
|
L

G

G

V

I

D

D

R

A

M

I

L

L

D
|
D

M

M

V

G

R
|
R

T

T

A

M

V

V

N

N

V

V

T

T

G

G

D

D

T

L

V

T

A

G

T

T

V

A

V

I

I

V

A

A

K

K

S

T

E

E

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (= G74), V83 (≠ L92), I157 (= I165), Y164 (≠ H172), K193 (≠ R193), T305 (= T307), I306 (= I308), I347 (= I349)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (= I15), M199 (= M199), S275 (= S276), T311 (= T313), G356 (= G358), L384 (= L380), D391 (= D387), R394 (= R390)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 4

6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
44% identity, 92% coverage: 14:415/437 of query aligns to 10:419/424 of 6zl4A

query
sites
6zl4A

K

K

I

I

L

L

I

W

G

G

M

L

G

V

A

L

G

G

I

A

L

V

I

F

G

G

L

L

L

I

L

-

R

A

N

G

F

H

F

F

G

G

G

Y

S

A

E

G

W

A

V

V

Q

K

D

T

Y

Y

I

I

T

K

E

P

G

-

F

-

H

-

V

-

I

F

G

G

T

D

I

L

F

F

I

V

N

R

S

L

L

L

K

K

M

M

L

L

V

V

V

M

P

P

L

I

V

V

F

L

I

A

S

S

L

L

V

V

C

V

G

G

T

A

C

A

S

S

L

I

S

S

E

-

P

P

S

A

K

R

L

L

G

G

R

R

L

V

G

G

G

V

K

K

T

I

L

V

A

V

F

Y

Y

Y

L

L

F

A

T

T

T

S

A

A

I

M

A

A

L

V

V

F

A

G

I

L

S

I

A

V

A

G

V

R

L

L

V

F

Q

N

P

V

G

G

-

A

N

N

A

V

S

N

L

L

A

G

S

S

E

G

S

T

M

G

Q

K

Y

A

S

I

A

E

K

A

E

Q

A

P

P

P

S

S

L

L

A

V

D

Q

V

T

L

L

I

L

N

N

I

I

V

V

P

P

S

T

N

N

P

P

M

F

K

A

A

S

L

L

S

A

E

K

G

G

N

E

M

V

L

L

Q

P

I

V

I

I

I

F

F

F

A

A

V

I

I

I

F

L

G

G

F

I

A

A

I

I

S

T

H

Y

I

L

-

M

-

N

-

R

-

N

G

E

E

E

R

R

G

V

R

R

R

K

V

S

A

A

A

E

-

T

-

L

-

R

L

V

F

F

D

D

D

G

L

L

N

A

E

E

V

A

I

M

M

Y

R
x
L

V

I

V

V

T

G

L
x
G

I

V

M

M

Q

Q

L

Y

A

A

P

P

Y

I

G

G

V

V

F

F

A

A

L

L

M

I

G

A

K

Y

L

V

A

M

L

A

T

E

L

Q

G

G

M

V

E

R

T

V

L

V

E

G

S

P

V

L

I

A

K

K

Y

V

F

V

M

G

L

A

V

V

L

Y

V

T

V

G

L

L

L

F

F

L

H

Q

G

I

F

V

V

I

V

T

Y

Y

P

F

T

I

L

L

K

K

L

V

F

F

S

-

G

G

L

I

S

D

P

P

L

I

M

K

F

F

I

I

R

R

K

K

M

A

R

K

D

D

V

A

Q

M

L

I

F

T

A

A

F

F

S

V

T

T

A
x
R

S
|
S

N

S

A

G

T

T

L

L

P

P

V

V

T

T

M

M

E
x
R

A

V

S

A

E

E

H

E

R

E

L

M

G

G

A

V

D

D

N

K

K

G

V

I

A

F

S

S

F

F

T

T

L

L

P

P

L

L

G

G

A

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T
|
T

A

A

I

L

M

Y

Q

Q

G

G

V

V

A

T

T

V

V

L

F

F

I

V

A

A

Q

N

V

A

F

I

G

G

I

H

D

P

L

L

T

T

I

L

T

G

D

Q

Y

Q

A

L

M

V

V

V

M

L

T

T

A

A

T

V

L

L

A

A

S

S

I

I

G

G

T

T

A

A

G

G

V

V

P

P

G
|
G

V
x
A

G

G

L

A

V

I

M

M

L

L

A

A

M

M

V

V

L

L

N

Q

Q

S

V

V

G

G

L

L

-

D

-

L

-

T

-

P

-

G

-

S

P

P

V

V

E

A

-

L

G

A

I

Y

A

A

L

M

I

I

L

L

G

G

V

I

D

D

R

A

M

I

L

L

D

D

M

M

V

G

R
|
R

T
|
T

A

M

V

V

N

N

V

V

T

T

G

G

D

D

T

L

V

A

A

G

T

T

V

V

V

I

I

V

A

A

K

K

S

T

E

E

G

K

A

E

L

L

N

D

E

E

A

S

binding decyl-beta-d-maltopyranoside: L191 (≠ R193), G195 (≠ L197), R282 (≠ E285)
binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ A274), S272 (= S275), S273 (= S276), M307 (= M310), T310 (= T313), G353 (= G356), A354 (≠ V357), R394 (= R390), T395 (= T391)

Sites not aligning to the query:

binding decyl-beta-d-maltopyranoside: 3

6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
44% identity, 92% coverage: 14:415/437 of query aligns to 11:420/425 of 6zgbA

query
sites
6zgbA

K

K

I

I

L

L

I

W

G

G

M

L

G

V

A

L

G

G

I

A

L

V

I

F

G

G

L

L

L

I

L

-

R

A

N

G

F

H

F

F

G

G

G

Y

S

A

E

G

W

A

V

V

Q

K

D

T

Y

Y

I

I

T

K

E

P

G

-

F

-

H

-

V

-

I

F

G

G

T

D

I

L

F

F

I

V

N

R

S

L

L

L

K

K

M

M

L

L

V

V

V

M

P

P

L

I

V

V

F

L

I

A

S

S

L

L

V

V

C

V

G

G

T

A

C

A

S

S

L

I

S

S

E

-

P

P

S

A

K

R

L

L

G

G

R

R

L

V

G

G

G

V

K

K

T

I

L

V

A

V

F

Y

Y

Y

L

L

F

A

T

T

T

S

A

A

I

M

A

A

L

V

V

F

A

G

I

L

S

I

A

V

A

G

V

R

L

L

V

F

Q

N

P

V

G

G

-

A

N

N

A

V

S

N

L

L

A

G

S

S

E

G

S

T

M

G

Q

K

Y

A

S

I

A

E

K

A

E

Q

A

P

P

P

S

S

L

L

A

V

D

Q

V

T

L

L

I

L

N

N

I

I

V

V

P

P

S

T

N

N

P

P

M

F

K

A

A

S

L

L

S

A

E

K

G

G

N

E

M

V

L

L

Q

P

I

V

I

I

I

F

F

F

A

A

V

I

I

I

F

L

G

G

F

I

A

A

I

I

S

T

H

Y

I

L

-

M

-

N

-

R

-

N

G

E

E

E

R

R

G

V

R

R

R

K

V

S

A

A

A

E

-

T

-

L

-

R

L

V

F

F

D

D

D

G

L

L

N

A

E

E

V

A

I

M

M

Y

R

L

V

I

V

V

T

G

L

G

I

V

M

M

Q

Q

L

Y

A

A

P

P

Y

I

G

G

V

V

F

F

A

A

L

L

M

I

G

A

K

Y

L

V

A

M

L

A

T

E

L

Q

G

G

M

V

E

R

T

V

L

V

E

G

S

P

V

L

I

A

K

K

Y

V

F

V

M

G

L

A

V

V

L

Y

V

T

V

G

L

L

L

F

F

L

H

Q

G

I

F

V

V

I

V

T

Y

Y

P

F

T

I

L

L

K

K

L

V

F

F

S

-

G

G

L

I

S

D

P

P

L

I

M

K

F

F

I

I

R

R

K

K

M

A

R

K

D

D

V

A

Q

M

L

I

F

T

A

A

F

F

S

V

T

T

A
x
R

S
|
S

N

S

A

G

T

T

L

L

P

P

V

V

T

T

M

M

E

R

A

V

S

A

E

E

H

E

R

E

L

M

G

G

A

V

D

D

N

K

K

G

V

I

A

F

S

S

F

F

T

T

L

L

P

P

L

L

G

G

A

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T

T

A

A

I

L

M

Y

Q

Q

G

G

V

V

A

T

T

V

V

L

F

F

I

V

A

A

Q

N

V

A

F

I

G

G

I

H

D

P

L

L

T

T

I

L

T

G

D

Q

Y

Q

A

L

M

V

V

V

M

L

T

T

A

A

T

V

L

L

A

A

S

S

I

I

G

G

T

T

A

A

G

G

V

V

P

P

G

G

V

A

G

G

L

A

V

I

M

M

L

L

A

A

M

M

V

V

L

L

N

Q

Q

S

V

V

G

G

L

L

-

D

-

L

-

T

-

P

-

G

-

S

P

P

V

V

E

A

-

L

G

A

I

Y

A

A

L

M

I

I

L

L

G

G

V

I

D

D

R

A

M

I

L

L

D
|
D

M

M

V

G

R
|
R

T
|
T

A

M

V

V

N

N

V

V

T

T

G

G

D

D

T

L

V

A

A

G

T

T

V

V

V

I

I

V

A

A

K

K

S

T

E

E

G

K

A

E

L

L

N

D

E

E

A

S

binding (2~{S},3~{S})-2-azanyl-3-[(2-nitrophenyl)methoxy]butanedioic acid: R272 (≠ A274), S273 (= S275), S274 (= S276), M308 (= M310), D392 (= D387), R395 (= R390), T396 (= T391)

6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
44% identity, 92% coverage: 14:415/437 of query aligns to 13:422/426 of 6xwnB

query
sites
6xwnB

K

K

I

I

L

L

I

W

G

G

M

L

G

V

A

L

G

G

I

A

L

V

I

F

G

G

L

L

L

I

L

-

R

A

N

G

F

H

F

F

G

G

G

Y

S

A

E

G

W

A

V

V

Q

K

D

T

Y

Y

I

I

T

K

E

P

G

-

F

-

H

-

V

-

I

F

G

G

T

D

I

L

F

F

I

V

N

R

S

L

L

L

K

K

M

M

L

L

V

V

V

M

P

P

L

I

V

V

F

L

I

A

S

S

L

L

V

V

C

V

G

G

T

A

C

A

S

S

L

I

S

S

E

-

P

P

S

A

K

R

L

L

G

G

R

R

L

V

G

G

G

V

K

K

T

I

L

V

A

V

F

Y

Y

Y

L

L

F

A

T

T

T

S

A

A

I

M

A

A

L

V

V

F

A

G

I

L

S

I

A

V

A

G

V

R

L

L

V

F

Q

N

P

V

G

G

-

A

N

N

A

V

S

N

L

L

A

G

S

S

E

G

S

T

M

G

Q

K

Y

A

S

I

A

E

K

A

E

Q

A

P

P

P

S

S

L

L

A

V

D

Q

V

T

L

L

I

L

N

N

I

I

V

V

P

P

S

T

N

N

P

P

M

F

K

A

A

S

L

L

S

A

E

K

G

G

N

E

M

V

L

L

Q

P

I

V

I

I

I

F

F

F

A

A

V

I

I

I

F

L

G

G

F

I

A

A

I

I

S

T

H

Y

I

L

-

M

-

N

-

R

-

N

G

E

E

E

R

R

G

V

R

R

R

K

V

S

A

A

A

E

-

T

-

L

-

R

L

V

F

F

D

D

D

G

L

L

N

A

E

E

V

A

I

M

M

Y

R

L

V

I

V

V

T

G

L

G

I

V

M

M

Q

Q

L

Y

A

A

P

P

Y

I

G

G

V

V

F

F

A

A

L

L

M

I

G

A

K

Y

L

V

A

M

L

A

T

E

L

Q

G

G

M

V

E

R

T

V

L

V

E

G

S

P

V

L

I

A

K

K

Y

V

F

V

M

G

L

A

V

V

L

Y

V

T

V

G

L

L

L

F

F

L

H

Q

G

I

F

V

V

I

V

T

Y

Y

P

F

T

I

L

L

K

K

L

V

F

F

S

-

G

G

L

I

S

D

P

P

L

I

M

K

F

F

I

I

R

R

K

K

M

A

R

K

D

D

V

A

Q

M

L

I

F

T

A

A

F

F

S

V

T

T

A

R

S
|
S

N

S

A

G

T

T

L

L

P

P

V

V

T

T

M

M

E

R

A

V

S

A

E

E

H

E

R

E

L

M

G

G

A

V

D

D

N

K

K

G

V

I

A

F

S

S

F

F

T

T

L

L

P

P

L

L

G

G

A

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T
|
T

A

A

I

L

M

Y

Q

Q

G

G

V

V

A

T

T

V

V

L

F

F

I

V

A

A

Q

N

V

A

F

I

G

G

I

H

D

P

L

L

T

T

I

L

T

G

D

Q

Y

Q

A

L

M

V

V

V

M

L

T

T

A

A

T

V

L

L

A

A

S

S

I

I

G

G

T

T

A

A

G

G

V

V

P

P

G

G

V

A

G

G

L

A

V

I

M

M

L

L

A

A

M

M

V

V

L

L

N

Q

Q

S

V

V

G

G

L

L

-

D

-

L

-

T

-

P

-

G

-

S

P

P

V

V

E

A

-

L

G

A

I

Y

A

A

L

M

I

I

L

L

G

G

V

I

D

D

R

A

M

I

L

L

D
|
D

M

M

V

G

R
|
R

T

T

A

M

V

V

N

N

V

V

T

T

G

G

D

D

T

L

V

A

A

G

T

T

V

V

V

I

I

V

A

A

K

K

S

T

E

E

G

K

A

E

L

L

N

D

E

E

A

S

binding (3s)-3-(benzyloxy)-l-aspartic acid: S275 (= S275), S276 (= S276), M310 (= M310), T313 (= T313), D394 (= D387), R397 (= R390)

5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
44% identity, 92% coverage: 14:415/437 of query aligns to 13:422/427 of 5e9sA

query
sites
5e9sA

K

K

I

I

L

L

I

W

G

G

M

L

G

V

A

L

G

G

I

A

L

V

I

F

G

G

L

L

L

I

L

-

R

A

N

G

F

H

F

F

G

G

G

Y

S

A

E

G

W

A

V

V

Q

K

D

T

Y

Y

I

I

T

K

E

P

G

-

F

-

H

-

V

-

I

F

G

G

T

D

I

L

F

F

I

V

N

R

S

L

L

L

K

K

M

M

L

L

V

V

V

M

P

P

L

I

V

V

F

L

I

A

S

S

L

L

V

V

C

V

G

G

T

A

C

A

S

S

L

I

S

S

E

-

P

P

S

A

K

R

L

L

G

G

R

R

L

V

G

G

G

V

K

K

T

I

L

V

A

V

F

Y

Y
|
Y

L

L

F

A

T
|
T

T
x
S

A

A

I

M

A

A

L

V

V

F

A

G

I

L

S

I

A

V

A

G

V

R

L

L

V

F

Q

N

P

V

G

G

-

A

N

N

A

V

S

N

L

L

A

G

S

S

E

G

S

T

M

G

Q

K

Y

A

S

I

A

E

K

A

E

Q

A

P

P

P

S

S

L

L

A

V

D

Q

V

T

L

L

I

L

N

N

I

I

V

V

P

P

S

T

N

N

P

P

M

F

K

A

A

S

L

L

S

A

E

K

G

G

N

E

M

V

L

L

Q

P

I

V

I

I

I

F

F

F

A

A

V

I

I

I

F

L

G

G

F

I

A

A

I

I

S

T

H

Y

I

L

-

M

-

N

-

R

-

N

G

E

E

E

R

R

G

V

R

R

R

K

V

S

A

A

A

E

-

T

-

L

-

R

L

V

F

F

D

D

D

G

L

L

N

A

E

E

V

A

I

M

M

Y

R
x
L

V

I

V

V

T

G

L
x
G

I

V

M

M

Q

Q

L
x
Y

A

A

P

P

Y

I

G

G

V

V

F

F

A

A

L

L

M

I

G

A

K

Y

L

V

A

M

L

A

T

E

L

Q

G

G

M

V

E

R

T

V

L

V

E

G

S

P

V

L

I

A

K

K

Y

V

F

V

M

G

L

A

V

V

L

Y

V

T

V

G

L

L

L

F

F

L

H

Q

G

I

F

V

V

I

V

T

Y

Y

P

F

T

I

L

L

K

K

L

V

F

F

S

-

G

G

L

I

S

D

P

P

L

I

M

K

F

F

I

I

R

R

K

K

M

A

R

K

D

D

V

A

Q

M

L

I

F

T

A

A

F

F

S

V

T

T

A
x
R

S
|
S

N

S

A

G

T

T

L

L

P

P

V

V

T

T

M

M

E

R

A

V

S

A

E

E

H

E

R

E

L

M

G

G

A

V

D

D

N

K

K

G

V

I

A

F

S

S

F

F

T

T

L

L

P

P

L

L

G
|
G

A
|
A

T
|
T

I

I

N
|
N

M
|
M

D
|
D

G

G

T
|
T

A

A

I

L

M

Y

Q

Q

G

G

V

V

A

T

T

V

V

L

F

F

I

V

A

A

Q

N

V

A

F

I

G

G

I

H

D

P

L

L

T

T

I

L

T

G

D

Q

Y

Q

A

L

M

V

V

V

M

L

T

T

A

A

T

V

L

L

A

A

S
|
S

I
|
I

G
|
G

T
|
T

A

A

G
|
G

V
|
V

P

P

G

G

V
x
A

G
|
G

L

A

V

I

M

M

L

L

A

A

M

M

V

V

L

L

N

Q

Q

S

V

V

G

G

L

L

-

D

-

L

-

T

-

P

-

G

-

S

P

P

V

V

E

A

-

L

G

A

I

Y

A

A

L

M

I

I

L

L

G

G

V

I

D

D

R

A

M

I

L

L

D
|
D

M

M

V

G

R
|
R

T
|
T

A

M

V

V

N
|
N

V
|
V

T

T

G

G

D
|
D

T

L

V

A

A

G

T

T

V

V

V

I

I

V

A

A

K

K

S

T

E

E

G

K

A

E

L

L

N

D

E

E

A

S

binding aspartic acid: R274 (≠ A274), S275 (= S275), S276 (= S276), T313 (= T313), G353 (= G353), V354 (= V354), A357 (≠ V357), G358 (= G358), D394 (= D387), R397 (= R390), T398 (= T391)
binding decyl-beta-d-maltopyranoside: L194 (≠ R193), G198 (≠ L197), Y202 (≠ L201)
binding sodium ion: Y87 (= Y95), T90 (= T98), S91 (≠ T99), S276 (= S276), G305 (= G305), A306 (= A306), T307 (= T307), N309 (= N309), N309 (= N309), M310 (= M310), D311 (= D311), S348 (= S348), I349 (= I349), G350 (= G350), T351 (= T351), N401 (= N394), V402 (= V395), D405 (= D398)

6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
44% identity, 92% coverage: 14:415/437 of query aligns to 6:411/416 of 6r7rA

query
sites
6r7rA

K

K

I

I

L

L

I

W

G

G

M

L

G

V

A

L

G

G

I

A

L

V

I

F

G

G

L

L

L

I

L

-

R

A

N

G

F
x
H

F

F

G

G

G

Y

S

A

E

G

W

A

V

V

Q

K

D

T

Y

Y

I

I

T

K

E

P

G

-

F

-

H

-

V

-

I

F

G

G

T

D

I

L

F

F

I

V

N

R

S

L

L

L

K

K

M

M

L

L

V

V

V

M

P

P

L

I

V

V

F

L

I

A

S

S

L

L

V

V

C

V

G

G

T

A

C

A

S

S

L

I

S

S

E

-

P

P

S

A

K

R

L

L

G

G

R

R

L

V

G

G

G

V

K

K

T

I

L

V

A

V

F

Y

Y

Y

L

L

F

A

T

T

T

S

A

A

I

M

A

A

L

V

V

F

A

G

I

L

S

I

A

V

A

G

V

R

L

L

V

F

Q

N

P

V

G

G

-

A

N

N

A

V

S

N

L

L

A

G

S

S

E

G

S

T

M

-

Q

-

Y

-

S

-

A

G

K

A

E

Q

A

P

P

P

S

S

L

L

A

V

D

Q

V

T

L

L

I

L

N

N

I

I

V

V

P

P

S

T

N

N

P

P

M

F

K

A

A

S

L

L

S

A

E

K

G

G

N

E

M

V

L

L

Q

P

I

V

I

I

I

F

F

F

A

A

V

I

I

I

F

L

G

G

F

I

A

A

I

I

S

T

H

Y

I

L

-

M

-

N

-

R

-

N

G

E

E

E

R

R

G

V

R

R

R

K

V

S

A

A

A

E

-

T

-

L

-

R

L

V

F

F

D

D

D

G

L

L

N

A

E

E

V

A

I

M

M

Y

R

L

V

I

V

V

T

G

L

G

I

V

M

M

Q

Q

L

Y

A

A

P

P

Y

I

G

G

V

V

F

F

A

A

L

L

M

I

G

A

K

Y

L

V

A

M

L

A

T

E

L

Q

G

G

M

V

E

R

T

V

L
x
V

E

G

S

P

V

L

I
x
A

K

K

Y

V

F

V

M

G

L

A

V

V

L

Y

V

T

V

G

L

L

L

F

F

L

H

Q

G

I

F

V

V

I

V

T

Y

Y

P

F

T

I

L

L

K

K

L

V

F

F

S

-

G

G

L

I

S

D

P

P

L

I

M

K

F

F

I

I

R

R

K

K

M

A

R

K

D

D

V

A

Q

M

L

I

F

T

A

A

F

F

S

V

T

T

A
x
R

S

S

S
|
S

N

S

A

G

T

T

L

L

P

P

V

V

T

T

M

M

E

R

A

V

S

A

E

E

H

E

R

E

L

M

G

G

A

V

D

D

N

K

K

G

V

I

A

F

S

S

F

F

T

T

L

L

P

P

L

L

G

G

A

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T
|
T

A

A

I

L

M

Y

Q

Q

G

G

V

V

A

T

T

V

V

L

F

F

I

V

A

A

Q

N

V

A

F

I

G

G

I

H

D

P

L

L

T

T

I

L

T

G

D

Q

Y

Q

A

L

M

V

V

V

M

L

T

T

A

A

T

V

L

L

A

A

S

S

I

I

G

G

T
|
T

A

A

G
|
G

V
|
V

P

P

G

G

V

A

G
|
G

L

A

V

I

M

M

L

L

A

A

M

M

V

V

L

L

N

Q

Q

S

V

V

G

G

L

L

-

D

-

L

-

T

-

P

-

G

-

S

P

P

V

V

E

A

-

L

G

A

I

Y

A

A

L

M

I

I

L

L

G

G

V

I

D

D

R

A

M

I

L

L

D
|
D

M

M

V

G

R
|
R

T
|
T

A

M

V

V

N
|
N

V

V

T

T

G

G

D

D

T

L

V

A

A

G

T

T

V

V

V

I

I

V

A

A

K

K

S

T

E

E

G

K

A

E

L

L

N

D

E

E

A

S

binding d-aspartic acid: R263 (≠ A274), S265 (= S276), M299 (= M310), T302 (= T313), T340 (= T351), G342 (= G353), V343 (= V354), G347 (= G358), D383 (= D387), R386 (= R390), T387 (= T391), N390 (= N394)
binding decyl-beta-d-maltopyranoside: H23 (≠ F32), V212 (≠ L222), A216 (≠ I226)

6bmiA Crystal structure of gltph r397c in complex with l-serine (see paper)
42% identity, 91% coverage: 14:409/437 of query aligns to 7:396/396 of 6bmiA

query
sites
6bmiA

K

K

I

I

L

L

I

I

G

G

M

L

G

I

A

L

G

G

I

A

L

I

I

V

G

G

L

L

L

I

L

L

R

G

N

H

F

Y

F

-

G

G

G

Y

S

A

E

D

W

A

V

V

Q

K

D

T

Y

Y

I

V

T

K

E

P

G

-

F

-

H

-

V

-

I

F

G

G

T

D

I

L

F

F

I

V

N

R

S

L

L

L

K

K

M

M

L

L

V

V

V

M

P

P

L

I

V

V

F

F

I

A

S

S

L

L

V

V

C

V

G

G

T

A

C

A

S

S

L

I

S

S

E

-

P

P

S

A

K

R

L

L

G

G

R

R

L

V

G

G

G

V

K

K

T

I

L

V

A

V

F

Y

Y

Y

L

L

F

L

T

T

T

S

A

A

I

F

A

A

L

V

V

T

V

L

A

G

I

I

S

I

A

M

A

A

V

R

L

L

V

F

Q

N

P

P

G

G

N

A

A

G

-

I

S

H

L

L

A

A

S

V

E

G

S

G

M

Q

Q

Q

Y

F

S

Q

A

P

K

K

E

Q

A

A

P

P

S

P

L

L

A

V

D

K

V

I

L

L

I

L

N

D

I

I

V

V

P

P

S

T

N

N

P

P

M

F

K

G

A

A

L

L

S

A

E

N

G

G

N

Q

M

V

L

L

Q

P

I

T

I

I

I

F

F

F

A

A

V

I

I

I

F

L

G

G

F

I

A

A

I

I

S

T

H

Y

I

L

-

M

-

N

-

S

-

E

G

N

E

E

R

K

G

V

R

R

R

K

V

S

A

A

-

E

A

T

L

L

F

L

D

D

D

A

L

I

N

N

-

G

-

L

-

A

E

E

V

A

I

M

M

Y

R

K

V

I

V

V

T

N

L

G

I

V

M

M

Q

Q

L

Y

A

A

P

P

Y

I

G

G

V

V

F

F

A

A

L

L

M

I

G

A

K

Y

L

V

A

M

L

A

T

E

L

Q

G

G

M

V

E

K

T

V

L

V

E

G

S

E

V

L

I

A

K

K

Y

V

F

T

M

A

L

A

V

V

L

Y

V

V

V

G

L

L

L

T

F

L

H

Q

G

I

F

L

V

L

V

V

Y

Y

P

F

T

V

L

L

K

K

L

I

F

Y

S

-

G

G

L

I

S

D

P

P

L

I

M

S

F

F

I

I

R

K

K

K

M

A

R

K

D

D

V

A

Q

M

L

L

F

T

A

A

F

F

S

V

T

T

A
x
R

S

S

S
|
S

N

S

A

G

T

T

L

L

P

P

V

V

T

T

M

M

E

R

A

V

S

A

E

K

H

E

R

-

L

M

G

G

A

I

D

S

N

E

K

G

V

I

A

Y

S

S

F

F

T

T

L

L

P

P

L

L

G

G

A

A

T

T

I

I

N

N

M

M

D

D

G

G

T
|
T

A

A

I

L

M

Y

Q

Q

G

G

V

V

A

C

T

T

V

F

F

F

I

I

A

A

Q

N

V

A

F

L

G

G

I

S

D

H

L

L

T

T

I

V

T

G

D

Q

Y

Q

A

L

M

T

V

I

V

V

M

L

T

T

A

A

T

V

L

L

A

A

S

-

I

-

G

-

T

-

A

-

G

-

V

-

P

-

G

-

V

-

G

-

L

-

V

I

M

M

L

L

A

A

M

M

V

V

L

L

N

E

Q

S

V

V

G

G

L

L

P

P

V

L

E

T

-

D

-

P

-

N

-

V

-

A

G

A

I

Y

A

A

L

M

I

I

L

L

G

G

V

I

D

D

R

A

M

I

L

L

D
|
D

M

M

V

G

R

C

T
|
T

A

M

V

V

N
|
N

V

V

T

T

G

G

D

D

T

L

V

T

A

G

T

T

V

A

V

I

I

V

A

A

K

K

S

T

E

E

binding serine: R268 (≠ A274), S270 (= S276), T306 (= T313), D374 (= D387), T378 (= T391), N381 (= N394)

7awmA Structure of the thermostabilized eaat1 cryst mutant in complex with l-asp, three sodium ions and the allosteric inhibitor ucph101 (see paper)
37% identity, 86% coverage: 22:398/437 of query aligns to 33:391/412 of 7awmA

query
sites
7awmA

G

G

I

V

L

V

I

L

G

G

L

F

L

L

R

R

N

P

F

Y

F

P

G

L

G

S

S

P

E

R

W

E

V

V

Q

K

D

-

Y

-

I

-

T

-

E

-

G

-

F

Y

F

F

H

A

V

F

I

P

G

G

T

E

I

L

F

L

I

M

N

R

S

M

L

L

K

K

M

M

L

L

V

I

V

L

P

P

L

L

V

I

F

V

I

S

S

S

L

L

V

I

C

T

G

G

T

L

C

A

S

S

L

L

S

D

E

A

P

K

S

A

K
x
S

L

-

G
|
G

R

R

L

L

G
|
G

G

M

K

R

T
x
A

L
x
V

A

V

F

Y

Y
|
Y

L

M

F

S

T

T

A

I

I

I

A

A

L

V

V

V

V

L

A

G

I

I

S

I

A

L

A

V

V

L

L

I

V

I

Q

H

P

P

G

-

N

-

A

-

S

-

L

-

A

-

S

-

E

-

S

-

M

-

Q

-

Y

-

S

-

A

-

K

E

E

V

A

L

P

D

S

C

L

F

A

L

D

D

V

L

L

A

I

R

N

N

I

I

V

F

P

P

S

S

N

N

P

L

M

V

K

S

A

A

L

A

-

F

-

R

-

S

-

Y

-

S

-

T

-

Q

-

E

S

V

E

E

G

G

-

M

N

N

M

I

L

L

Q

G

I

L

I

V

F

F

A

S

V
x
M

I

V

F

F

G

G

F
|
F

A

A

I

L

S

G

H

K

I

M

G

G

E

E

R

Q

G

G

R

Q

R

L

V

L

A

V

A

D

L

F

F

F

D

N

D

S

L

L

N

N

E

E

V

A

I

T

M

M

R

K

V

L

V

V

T

A

L

I

I

I

M

M

Q

W

L

Y

A

A

P

P

Y

L

G

G

V

I

-

L

F

F

A

L

L

I

M

A

G

G

K

K

L

I

-

V

A

G

L

G

T

Q

L

L

G

G

M

M

E

-

T

-

L

-

E

-

S

-

V

-

I

-

K

-

Y

Y

F

M

M

V

L

T

V

V

L

I

V

V

V

G

L

L

L

V

F

I

H

H

G

G

F

L

V

I

V

V

Y

L

P

P

T

L

L

I

L

Y

K

F

L

L

F

I

S

T

G

R

L

K

S

N

P

P

L

F

M

V

F

F

I

I

R

A

K

G

M

I

R

L

D

Q

V

A

Q

L

L

I

F

T

A

A

F

L

S

G

T

T

A

S

S
|
S

N

S

A

A

T

T

L

L

P

P

V

I

T

T

M

F

E

K

A

C

S

L

E

E

H

E

R

N

L

N

G

G

A

V

D

D

N

K

K

R

V

I

A

T

S

R

F
|
F

T

V

L

L

P

P

L
x
V

G

G

A

A

T

T

I

I

N

N

M

M

D

D

G

G

T
|
T

A

A

I

L

M

Y

Q

E

G

A

V

V

A

A

T

A

V

I

F

F

I

I

A

A

Q

Q

V

V

F

N

G

N

I

Y

D

E

L

L

T

D

I

F

T

G

D

Q

Y

I

A

I

M

T

V

I

V

S

M

I

T

T

A

A

T

T

L

A

A

A

S

S

I

I

G

G

T

A

A

A

G
|
G

V
x
I

P

P

G

Q

V
x
A

G
|
G

L

L

V

V

M

T

L

M

A

V

M

I

V

V

L

L

N

T

Q

A

V

V

G

G

L

L

P

P

V

T

E

D

G

D

I

I

A

T

L

L

I

I

L

I

G

A

V

V

D

D

R

W

M

L

L

L

D
|
D

M

R

V

F

R
|
R

T
|
T

A

M

V

V

N

N

V

V

T

L

G

G

D

D

binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: S88 (≠ K83), G89 (= G85), G92 (= G88), A95 (≠ T91), V96 (≠ L92), Y99 (= Y95), M163 (≠ V164), F167 (= F168), F293 (= F300), V297 (≠ L304)
binding aspartic acid: S268 (= S275), S269 (= S276), T306 (= T313), G346 (= G353), I347 (≠ V354), A350 (≠ V357), G351 (= G358), D380 (= D387), R383 (= R390), T384 (= T391)

Q10901 Excitatory amino acid transporter; Sodium-dependent glutamate/ aspartate transporter from Caenorhabditis elegans (see paper)
34% identity, 83% coverage: 52:413/437 of query aligns to 56:465/503 of Q10901

query
sites
Q10901

G

G

T

E

I

L

F

L

I

M

N

H

S

M

L

L

K

K

M

M

L

M

V

I

V

L

P

P

L

L

V

I

F

M

I

S

S

S

L

L

V

I

C

S

G

G

T

L

C

A

S

Q

L

L

S

-

E

D

P

A

S

R

K

Q

L

S

G

G

R

K

L

L

G

G

G

S

K

L

T

A

L

V

A

T

F

Y

Y

Y

L

M

F

F

T

T

A

A

I

V

A

A

L

V

V

V

V

T

A

G

I

I

S

F

A

L

A

V

V

L

L

V

V

I

Q

H

P

P

G

G

N

D

A

P

S

T

L

I

A

K

S

K

E

E

-

I

-

G

-

T

-

G

S

T

M

E

Q

G

Y

K

S

T

A

V

K

S

E

T

A

V

P

D

S

T

L

L

A

L

D

D

V

L

L

L

I

R

N

N

I

M

V

F

P

P

S

E

N

N

-

V

-

Q

-

A

-

T

-

F

-

Q

-

V

-

Q

-

T

-

K

-

Y

-

I

-

K

-

V

-

R

-

P

-

K

-

V

-

K

-

N

-
x
N

-

D

-

S

-

A

P

T

M

L

K

A

A

A

L

L

S

N

E
x
N

G

G

-

S

-

L

-

D

-

Y

-

V

-

K

-

A

-

S

-

V

-

E

-

Y

-

T

-

S

-

G

-

M

N

N

M

V

L

L

Q

G

I

V

I

I

I

V

F

F

A

C

V

I

I

A

F

I

G

G

F

I

A

S

I

L

S

S

H

Q

I

L

G

G

E

Q

R

E

G

A

R

H

R

V

V

M

A

V

A

Q

L

F

F

F

D

V

D

I

L

M

N

D

E

K

V

V

I

I

M

M

R

K

V

L

V

V

T

M

L

T

I

V

M

M

Q

W

L

Y

A

S

P

P

Y

F

G

G

V

I

F

F

A

C

L

L

-

I

M

M

G

G

K

K

-

I

L

L

A

E

L

I

T

H

L

D

G

L

M

A

E

D

T

T

L

A

E

R

S

M

V

L

I

A

K

M

Y

Y

F

M

M

V

L

T

V

V

L

L

V

S

V

G

L

L

L

A

F

I

H

H

G

S

F

L

V

I

V

S

Y

L

P

P

T

L

L

I

L

F

K

F

L

V

F

T

S

T

G

K

L

K

S

N

P

P

L

Y

M

V

F

F

I

M

R

R

K

G

M

L

R

F

D

Q

V

A

Q

W

L

I

F

T

A

A

F

L

S

G

T

T

A

A

S

S

N

S

A

A

T

T

L

L

P

P

V

I

T

T

M

F

E

N

A

C

S

L

E

E

H

E

R

N

L

L

G

G

A

V

D

D

N

R

K

R

V

V

A

T

S

R

F

F

T

V

L

L

P

P

L

V

G

G

A

A

T

T

I

I

N

N

M

M

D

D

G

G

T

T

A

A

I

L

M

Y

Q

E

G

A

V

V

A

A

T

A

V

I

F

F

I

I

A

A

Q

Q

V

I

F

N

G

G

I

V

D

H

L

L

T

S

I

F

T

G

D

Q

Y

V

A

V

M

T

V

V

V

S

M

L

T

T

A

A

T

T

L

L

A

A

S

S

I

I

G

G

T

A

A

A

G

S

V

V

P

P

G

S

V

A

G

G

L

L

V

V

M

T

L

M

A

L

M

L

V

V

L

L

N

T

Q

A

V

V

G

G

L

L

P

P

V

V

E

K

G

D

I

V

A

S

L

L

I

I

L

V

G

A

V

V

D

D

R

W

M

L

L

L

D

D

M

R

V

I

R

R

T

T

A

S

V

I

N

N

V

V

T

L

G

G

D

D

T

A

V

M

-

G

A

A

T

G

V

I

V

V

I

Y

A

H

K

Y

S

S

E

K

G

A

A

D

L

L

N

D

N177 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine
N187 (≠ E153) modified: carbohydrate, N-linked (GlcNAc...) asparagine

5mjuA Structure of the thermostabilized eaat1 cryst mutant in complex with the competititve inhibitor tfb-tboa and the allosteric inhibitor ucph101 (see paper)
37% identity, 86% coverage: 22:398/437 of query aligns to 25:377/397 of 5mjuA

query
sites
5mjuA

G

G

I

V

L

V

I

L

G

G

L

F

L

L

R

R

N

P

F

Y

F

P

G

L

G

S

S

P

E

R

W

E

V

V

Q

K

D

-

Y

-

I

-

T

-

E

-

G

-

F

Y

F

F

H

A

V

F

I

P

G

G

T

E

I

L

F

L

I

M

N

R

S

M

L

L

K

K

M

M

L

L

V

I

V

L

P

P

L

L

V
x
I

F

V

I

S

S

S

L

L

V

I

C

T

G

G

T
x
L

C

A

S

S

L

L

S

D

E

A

P

K

S

A

K
x
S

L

-

G
|
G

R

R

L

L

G
|
G

G

M

K

R

T

A

L

V

A

V

F

Y

Y
|
Y

L

M

F

S

T

T

A

I

I

I

A

A

L

V

V

V

V

L

A

G

I

I

S

I

A

L

A

V

V

L

L

I

V

I

Q

H

P

-

G

-

N

-

A

-

S

-

L

-

A

-

S

-

E

-

S

-

M

-

Q

-

Y

-

S

-

A

-

K

E

E

V

A

L

P

D

S

C

L

F

A

L

D

D

V

L

L

A

I

R

N

N

I

I

V

F

P

P

S

S

N

N

P

L

M

V

K

S

A

A

L

A

-

F

-

R

-

S

-

Y

-

S

-

T

-

G

-

Q

-

E

S

V

E

E

G

G

-

M

N

N

M
x
I

L

L

Q

G

I

L

I

V

F

F

A

S

V
x
M

I

V

F

F

G

G

F
|
F

A

A

I

L

S

G

H

K

I

M

G

G

E

E

R

Q

G

G

R

Q

R

L

V

L

A

V

A

D

L

F

F

F

D

N

D

S

L

L

N

N

E

E

V

A

I

T

M

M

R

K

V

L

V

V

T

A

L

I

I

I

M

M

Q

W

L

Y

A

A

P

P

Y

L

G

G

V

I

-

L

F

F

A

L

L

I

M

A

G

G

K

K

L

I

A

V

L

E

-

G

T

Q

L

L

G

G

M

M

E

-

T

-

L

-

E

-

S

-

V

-

I

-

K

-

Y

Y

F

M

M

V

L

T

V

V

L

I

V

V

V

G

L

L

L

V

F

I

H

H

G

G

F

L

V

I

V

V

Y

L

P

P

T

L

L

I

L

Y

K

F

L

L

F

I

S

T

G

R

L

K

S

N

P

P

L

F

M

V

F

F

I

I

R

A

K

G

M

I

R

L

D

Q

V

A

Q

L

L

I

F

T

A

A

F

L

S

G

T

T

A

S

S

S

S
|
S

N
x
S

A

A

T

T

L

L

P

P

V

I

T

T

M

F

E

K

A

C

S

L

E

E

H

E

R

N

L

N

G

G

A

V

D

D

N

K

K

R

V

I

A

T

S

R

F
|
F

T

V

L

L

P

P

L
x
V

G

G

A
|
A

T
|
T

I

I

N

N

M
|
M

D

D

G

G

T
|
T

A

A

I

L

M

Y

Q

E

G

A

V

V

A

A

T

A

V

I

F

F

I

I

A

A

Q

Q

V

V

F

N

G

Q

I

I

D

-

L

I

T

T

I

I

T

S

D

-

Y

-

A

-

M

-

V

-

M

I

T

T

A

A

T

T

L

A

A

A

S

S

I

I

G
|
G

T

A

A

A

G

G

V

I

P

P

G

Q

V
x
A

G
|
G

L

L

V

V

M

T

L

M

A

V

M

I

V

V

L

L

N

T

Q

A

V

V

G

G

L

L

P

P

V

T

E

D

G

D

I

I

A

T

L

L

I

I

L

I

G

A

V

V

D

D

R

W

M

L

L

L

D
|
D

M

R

V

F

R
|
R

T

T

A

M

V

V

N
|
N

V

V

T

L

G

G

D

D

binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: L72 (≠ T75), S80 (≠ K83), G81 (= G85), G84 (= G88), Y91 (= Y95), M156 (≠ V164), F160 (= F168), F286 (= F300), V290 (≠ L304)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I64 (≠ V67), I148 (≠ M156), S262 (= S276), S263 (≠ N277), A292 (= A306), T293 (= T307), M296 (= M310), T299 (= T313), G329 (= G350), A336 (≠ V357), G337 (= G358), D366 (= D387), R369 (= R390), N373 (= N394)

P43006 Excitatory amino acid transporter 2; GLT-1; Sodium-dependent glutamate/aspartate transporter 2; Solute carrier family 1 member 2 from Mus musculus (Mouse) (see paper)
32% identity, 88% coverage: 52:435/437 of query aligns to 82:528/572 of P43006

query
sites
P43006

G

G

T

D

I

I

F

L

I

M

N

R

S

M

L

L

K

K

M

M

L

L

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I

V

L

P

P

L

L

V

I

F

I

I

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S

S

L

L

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I

C

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G

G

T

L

C

S

S

G

L

L

S

D

E

A

P

K

S

A

K

S

L

-

G

G

R

R

L

L

G

G

G

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K

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T

A

L

M

A

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Y

Y

Y

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A

I

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A

A

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A

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G

I

V

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I

A

L

A

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L

L

A

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H

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P

G

G

N

N

A

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S

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L

L

A

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S

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E

Q

-

L

-

G

-

P

-

G

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K

-

N

-

D

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E

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S

-

L

-

D

-

A

-

F

-

L

-

D

-

L

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N

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F

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P

-

E

-

N

-

L

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Q

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A

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C

-

F

-

Q

S

Q

M

I

Q

Q

Y

T

S

V

A

T

K

K

E

K

-

V

-

L

-

V

A

A

P

P

-

P

-

S

-

E

-

A

-

N

-

T

-

K

-

A

-

V

-

I

S

S

L

M

A

L

D

N

V

E

L

T

I

M

N

N

I

E

V

A

P

P

S

E

N

E

P

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M

K

K

I

A

V

L

I

S

K

E

K

G

G

-

L

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E

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F

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D

-

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-

M

N

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M

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Q

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F

F

A

F

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I

I

A

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F

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G

F

I

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A

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K

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Q

G

A

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M

A

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A

E

L

F

F

F

D

N

D

I

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L

N

N

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E

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I

I

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M

M

R

K

V

L

V

V

T

I

L

M

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M

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Q

W

L

Y

A

S

P

P

Y

L

G

G

V

I

F

A

A

C

L

L

M

I

-

C

G

G

K

K

L

I

A

I

L

A

T

I

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K

G

D

M

L

E

E

T

V

L

V

E

A

S

R

V

Q

I

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K

G

Y

M

F

Y

M

M

L

I

V

T

L

V

V

I

V

V

-

G

L

L

L

I

F

I

H

H

G

G

F

G

V

I

V

F

Y

L

P

P

T

L

L

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F

L

V

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T

G

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N

P

P

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F

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I

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A

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L

I

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A

A

F

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T

A

A

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A

A

G

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L

P

P

V

V

T

T

M

F

E

R

A

C

S

L

E

E

H

D

R

N

L

L

G

G

A

I

D

D

N

K

K

R

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V

A

T

S

R

F

F

T

V

L

L

P

P

L

V

G

G

A

A

T

T

I

I

N

N

M

M

D

D

G

G

T

T

A

A

I

L

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Y

Q

E

G

A

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V

A

A

T

A

V

I

F

F

I

I

A

A

Q

Q

V

M

F

N

G

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D

I

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I

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M

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A

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I

I

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A

A

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A

G

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L

L

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V

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T

L

M

A

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M

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L

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T

Q

A

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V

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G

L

L

P

P

V

T

E

E

G

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I

I

A

S

L

L

I

L

L

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G

A

V

V

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D

R

W

M

L

L

L

D

D

M

R

V

M

R

R

T

T

A

S

V

V

N

N

V

V

T

V

G

G

D

D

T

S

V

F

-

G

A

A

T

G

V

I

V

V

I

Y

A

H

K

L

S

S

E

K

G

S

A

E

L

L

N

D

-

T

-

I

-

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-

S

-

Q

E

H

A

R

V

M

F

Q

N

E

D

D

P

I

K

E

A

M

G

T

K

K

T

T

A

Q

G

S

S

I

F

Y

D

D

A

D

E

K

V

N

H

H

R

R

Sites not aligning to the query:

38 modified: S-palmitoyl cysteine; C→S: Severely impairs glutamate uptake activity.

P43003 Excitatory amino acid transporter 1; Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3 from Homo sapiens (Human) (see 3 papers)
33% identity, 87% coverage: 22:400/437 of query aligns to 61:489/542 of P43003

query
sites
P43003

G

G

I

T

L

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I

L

G

G

L

F

L

T

L

L

R

R

N

-

F

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-

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Y

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M

D

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Y

Y

I

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F

Y

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M

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Q

M

M

L

L

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L

P

P

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V

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F

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I

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S

S

L

L

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C

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G

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C

A

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A

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D

E

S

P

K

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A

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-

G

G

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M

G

G

G

M

K

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T

A

L

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A

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F

Y

Y

Y

L

M

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T

T

T

A

I

I

I

A

A

L

V

V

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I

A

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I

S

I

A

I

A

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I

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P

P

G

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N

K

A

G

S

T

L

-

A

-

S

K

E

E

S

N

M

M

Q

H

Y

R

S

E

A

G

K

K

-

I

-

V

-

R

-

V

-

T

E

A

A

A

P

D

S

A

L

F

A

L

D

D

V

L

L

I

I

R

N

N

I

M

V

F

P

P

S

P

N

N

-

L

-

V

-

E

-

A

-

C

-

F

-

K

-

Q

-

F

-

K

-

T

-

N

-

Y

-

E

-

K

-

R

-

S

-

F

-

K

-

V

P

P

M

I

K

Q

A

A

-

N

-

E

-

T

-

L

-

V

-

G

-

A

-

V

-

I

-

N

L

V

S

S

E

E

G

A

-

M

-

E

-

T

-

L

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T

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-

I

-

T

-

E

-

L

-

V

-

P

-

V

-

P

-

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-

S

-

V

-

N

-

G

-

V

N

N

M

A

L

L

Q

G

I

L

I

V

F

F

A

S

V

M

I

C

F

F

G

G

F

F

A

V

I

I

S

G

H

N

I

M

G

K

E

E

R

Q

G

G

R

Q

R

A

V

L

A

R

A

E

L

F

F

F

D

D

D

S

L

L

N

N

E

E

V

A

I

I

M

M

R

R

V

L

V

V

T

A

L

V

I

I

M

M

Q

W

L

Y

A

A

P

P

Y

V

G

G

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I

-

L

F

F

A

L

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A

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K

L

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A

V

L

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M

L

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M

M

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G

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A

-

M

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Y

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V

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L

I

V

V

V

G

L

L

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F

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P

T

L

L

L

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L

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P

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L

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L

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A

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G

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A
x
S

S
|
S

N

S

A

A

T

T

L

L

P

P

V

I

T

T

M

F

E

K

A

C

S

L

E

E

H

E

R

N

L

N

G

G

A

V

D

D

N

K

K

R

V

V

A

T

S

R

F

F

T

V

L

L

P

P

L

V

G

G

A

A

T
|
T

I

I

N

N

M

M

D

D

G

G

T
|
T

A

A

I

L

M

Y

Q

E

G

A

V

L

A

A

T

A

V

I

F

F

I

I

A

A

Q

Q

V

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F

N

G

N

I

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E

L

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N

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I

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S

M

I

T

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A

A

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T

L

A

A

A

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S

I

I

G

G

T

A

A

A

G

G

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x
I

P
|
P

G
x
Q

V
x
A

G
|
G

L

L

V

V

M

T

L

M

A

V

M

I

V

V

L

L

N

T

Q

S

V

V

G

G

L

L

P

P

V

T

E

D

G

D

I

I

A

T

L

L

I

I

L

I

G

A

V

V

D

D

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W

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F

L

L

D
|
D

M
x
R

V

L

R

R

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T

A

T

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T

N
|
N

V

V

T

L

G

G

D

D

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S

V

L

S363 (≠ A274) mutation to R: Loss of electrogenic glutamate transport. Strongly decreased L-aspartate and L-glutamate uptake combined with strongly increased permeability ot other ions; when associated with M-477.
SSS 363:365 (≠ ASS 274:276) binding
T396 (= T307) binding
T402 (= T313) binding
IPQAG 443:447 (≠ VPGVG 354:358) binding
D476 (= D387) binding
R477 (≠ M388) mutation to M: Strongly decreased L-aspartate and L-glutamate uptake combined with strongly increased permeability ot other ions; when associated with R-363.
N483 (= N394) binding

Sites not aligning to the query:

523 Y→F: No effect on activity.

P56564 Excitatory amino acid transporter 1; Glial high affinity glutamate transporter; High-affinity neuronal glutamate transporter; GluT-1; Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3 from Mus musculus (Mouse) (see paper)
33% identity, 87% coverage: 22:400/437 of query aligns to 61:489/543 of P56564

query
sites
P56564

G

G

I

T

L

I

I

L

G

G

L

F

L

A

L

L

R

R

N

-

F

-

G

-

S

-

E

P

W

Y

V

K

Q

M

D

S

Y

Y

I

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Y

F

F

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N

R

S

M

L

L

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Q

M

M

L

L

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L

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P

L

L

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F

I

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S

L

L

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C

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M

C

A

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A

L

L

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D

E

S

P

K

S

A

K

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L

-

G

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R

K

L

M

G

G

G

M

K

R

T

A

L

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A

V

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Y

Y

Y

L

M

F

T

T

T

A

I

I

I

A

A

L

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V

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V

I

A

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I

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S

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A

I

A

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V

I

L

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Q

H

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P

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G

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K

A

G

S

T

L

-

A

-

S

K

E

E

S

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M

M

Q

Y

Y

R

S

E

A

G

K

K

-

I

-

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E

A

A

A

P

D

S

A

L

F

A

L

D

D

V

L

L

I

I

R

N

N

I

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P

S

P

N

N

P

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E

A

A

-

C

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K

-

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F

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R

-

S

-

F

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-

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x
N

-

E

-

T

-

L

-

G

-

A

-

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-

I

-

N

-
x
N

L

V

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S

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E

G

A

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M

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-

R

-

E

-

M

-

V

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P

-

V

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P

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G

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-

V

-

N

-

G

-

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N

N

M

A

L

L

Q

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L

I

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F

F

A

S

V

M

I

C

F

F

G

G

F

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H

N

I

M

G

K

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Q

G

G

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Q

R

A

V

L

A

R

A

E

L

F

F

F

D

D

D

S

L

L

N

N

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E

V

A

I

I

M

M

R

R

V

L

V

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A

L

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L

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A

A

P

P

Y

L

G

G

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-

L

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A

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A

G

G

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K

L

I

A

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M

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A

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M

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M

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V

L

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A

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L

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K

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L

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N

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F

T

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L

L

P

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V

F

N

G

N

I

F

D

D

L

L

T

N

I

F

T

G

D

Q

Y

I

A

I

M

T

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I

V

S

M

I

T

T

A

A

T

T

L

A

A

A

S

S

I

I

G

G

T

A

A

A

G

G

V

I

P

P

G

Q

V

A

G

G

L

L

V

V

M

T

L

M

A

V

M

I

V

V

L

L

N

T

Q

S

V

V

G

G

L

L

P

P

V

T

E

D

G

D

I

I

A

T

L

L

I

I

L

I

G

A

V

V

D

D

R

W

M

F

L

L

D

D

M

R

V

L

R

R

T

T

A

T

V

T

N

N

V

V

T

L

G

G

D

D

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S

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L

N206 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine
N216 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine

P31596 Excitatory amino acid transporter 2; GLT-1; Sodium-dependent glutamate/aspartate transporter 2; GLUT-R; Solute carrier family 1 member 2 from Rattus norvegicus (Rat) (see paper)
32% identity, 88% coverage: 52:435/437 of query aligns to 82:529/573 of P31596

query
sites
P31596

G

G

T

D

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A

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A

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-

G

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T

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Y

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I

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S

I

A

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A

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L

A

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G

N

N

A

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K

L

L

A

K

S

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E

Q

-

L

-

G

-

P

-

G

-

K

-

N

-

D

-

E

-

V

-

S

-

L

-

D

-

A

-

F

-

L

-

D

-

L

-

I

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R

-

N

-

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F

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-

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-

N

-

L

-

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-

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-

A

-

C

-

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M

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Y

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A

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K

E

K

-

V

-

L

-

V

A

A

P

P

-

P

-

S

-

E

-

A

-

N

-

T

-

K

-

A

-

V

-

I

S

S

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A

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D

N

V

E

L

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N

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A

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E

N

E

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T

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E

K

G

G

-

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-

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A

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|
K

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M

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H

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A

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I

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N

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N

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-

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V

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E

K

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E

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T

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N

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H

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R

K298 (= K212) mutation K->H,R: Normal transporter activity.; mutation K->N,T: Reduced transporter activity.
H326 (= H239) mutation H->N,T,K,R: No transporter activity.

7xr6A Structure of human excitatory amino acid transporter 2 (eaat2) in complex with way-213613 (see paper)
34% identity, 82% coverage: 52:409/437 of query aligns to 47:417/424 of 7xr6A

query
sites
7xr6A

G

G

T

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A

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A

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R
|
R

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x
R

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Y

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|
I

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I

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G

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N

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A

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M

-

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-

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A

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A

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A

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L

L

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R

N

N

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E

N

N

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A

A

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-

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-

I

-

K

-

G

-

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-

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-

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N

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G

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L

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F

A

F

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I

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A

F

F

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G

F

I

A

A

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Q

G

A

R

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A

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N

D

I

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L

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N

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I

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M

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P

Y

L

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G

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A

A

C

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C

G

G

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A

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I

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M

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E

E

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I

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K

G

Y

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Y

M

M

L

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L

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L

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L

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I

H

I

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H

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L

L
x
I

L

Y

K

F

L

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N

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L

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A

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A

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|
S

N

A

A

G

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T

L

L

P

P

V

V

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T

M

F

E

R

A

C

S

L

E

E

H

E

R

N

L

L

G

G

A

I

D

D

N

K

K

R

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V

A

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R

F

F

T

V

L

L

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P

L

V

G

G

A

A

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T

I

I

N

N

M

M

D

D

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|
T

A

A

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V

A

A

T

A

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F

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T

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L

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|
G

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x
M

A

L

M

L

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I

L

L

N

T

Q

A

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G

L

L

P

P

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E

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I

A

S

L

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x
V

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D
|
D

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x
W

M

L

L

L

D

D

M

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M

R
|
R

T
|
T

A

S

V

V

N

N

V

V

T

V

G

G

D

D

T

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F

A

G

T

A

V

G

V

I

I

V

-

Y

-

H

-

L

A

S

K

K

S

S

E

E

binding (2S)-2-azanyl-4-[[4-[2-bromanyl-4,5-bis(fluoranyl)phenoxy]phenyl]amino]-4-oxidanylidene-butanoic acid: S280 (= S275), S281 (= S276), T318 (= T313), G363 (= G358), M367 (≠ L362), V385 (≠ L380), D388 (= D383), R395 (= R390), T396 (= T391)
binding dodecyl beta-D-glucopyranoside: W389 (≠ R384)
binding cholesterol hemisuccinate: R80 (= R86), R84 (≠ K90), I95 (= I101), I252 (≠ L247)

Sites not aligning to the query:

binding dodecyl beta-D-glucopyranoside: 16, 19, 20

Query Sequence

>6937149 FitnessBrowser__SB2B:6937149
MAAAQSSKIGLTGKILIGMGAGILIGLLLRNFFGGSEWVQDYITEGFFHVIGTIFINSLK
MLVVPLVFISLVCGTCSLSEPSKLGRLGGKTLAFYLFTTAIALVVAISAAVLVQPGNASL
ASESMQYSAKEAPSLADVLINIVPSNPMKALSEGNMLQIIIFAVIFGFAISHIGERGRRV
AALFDDLNEVIMRVVTLIMQLAPYGVFALMGKLALTLGMETLESVIKYFMLVLVVLLFHG
FVVYPTLLKLFSGLSPLMFIRKMRDVQLFAFSTASSNATLPVTMEASEHRLGADNKVASF
TLPLGATINMDGTAIMQGVATVFIAQVFGIDLTITDYAMVVMTATLASIGTAGVPGVGLV
MLAMVLNQVGLPVEGIALILGVDRMLDMVRTAVNVTGDTVATVVIAKSEGALNEAVFNDP
KAGKTAGSFDAEVHRGE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory