SitesBLAST
Comparing 6937267 FitnessBrowser__SB2B:6937267 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6ed7A Crystal structure of 7,8-diaminopelargonic acid synthase bound to inhibitor mac13772
57% identity, 95% coverage: 4:417/436 of query aligns to 5:417/429 of 6ed7A
- active site: Y17 (= Y16), Y144 (= Y144), D245 (= D245), K274 (= K274)
- binding 2-[(2-nitrophenyl)sulfanyl]acetohydrazide: Y17 (= Y16), W52 (= W51), W52 (= W51), Y144 (= Y144), D147 (= D147), A217 (= A217), K274 (= K274), R391 (= R391), F393 (= F393), F393 (= F393)
- binding pyridoxal-5'-phosphate: G112 (= G111), S113 (= S112), Y144 (= Y144), H145 (= H145), D245 (= D245), I247 (= I247), K274 (= K274)
P12995 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase; 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; Diaminopelargonic acid synthase; EC 2.6.1.62 from Escherichia coli (strain K12) (see 4 papers)
57% identity, 95% coverage: 4:417/436 of query aligns to 5:417/429 of P12995
- Y17 (= Y16) mutation to F: Severely reduces the aminotransferase activity.
- W52 (= W51) binding
- GS 112:113 (= GS 111:112) binding
- Y144 (= Y144) mutation to F: Severely reduces the aminotransferase activity.
- D147 (= D147) mutation to N: Loss of aminotransferase activity.
- D245 (= D245) binding
- R253 (= R253) mutation to A: Has only a small effect on the rate of reaction with DAPA.; mutation to K: Increases aminotransferase activity toward SAM.; mutation to M: Loss of aminotransferase activity.; mutation to Q: Increases aminotransferase activity toward SAM.
- K274 (= K274) binding ; modified: N6-(pyridoxal phosphate)lysine
- G307 (= G307) binding
- PT 308:309 (= PT 308:309) binding
- R391 (= R391) binding ; mutation to A: Reduces aminotransferase activity.
1mlzA Crystal structure of 7,8-diaminopelargonic acid synthase in complex with the trans-isomer of amiclenomycin. (see paper)
57% identity, 95% coverage: 4:417/436 of query aligns to 5:416/427 of 1mlzA
- active site: Y17 (= Y16), Y144 (= Y144), E210 (= E211), D244 (= D245), A247 (= A248), K273 (= K274), Y397 (= Y398)
- binding pyridoxal-5'-phosphate: G112 (= G111), S113 (= S112), Y144 (= Y144), H145 (= H145), D244 (= D245), I246 (= I247), K273 (= K274), P307 (= P308), T308 (= T309)
- binding trans-amiclenomycin: W52 (= W51), W53 (= W52), Y144 (= Y144), K273 (= K274), R390 (= R391), F392 (= F393)
1mlyA Crystal structure of 7,8-diaminopelargonic acid synthase in complex with the cis isomer of amiclenomycin (see paper)
57% identity, 95% coverage: 4:417/436 of query aligns to 5:416/427 of 1mlyA
- active site: Y17 (= Y16), Y144 (= Y144), E210 (= E211), D244 (= D245), A247 (= A248), K273 (= K274), Y397 (= Y398)
- binding cis-amiclenomycin: W52 (= W51), W53 (= W52), K273 (= K274), R390 (= R391), F392 (= F393)
- binding pyridoxal-5'-phosphate: G112 (= G111), S113 (= S112), Y144 (= Y144), H145 (= H145), D244 (= D245), I246 (= I247), K273 (= K274), P307 (= P308), T308 (= T309)
1dtyA Crystal structure of adenosylmethionine-8-amino-7-oxonanoate aminotransferase with pyridoxal phosphate cofactor.
57% identity, 95% coverage: 4:417/436 of query aligns to 5:417/429 of 1dtyA
- active site: Y17 (= Y16), Y144 (= Y144), E211 (= E211), D245 (= D245), A248 (= A248), K274 (= K274), Y398 (= Y398)
- binding pyridoxal-5'-phosphate: G112 (= G111), S113 (= S112), Y144 (= Y144), H145 (= H145), D245 (= D245), I247 (= I247), K274 (= K274)
4cxqA Mycobaterium tuberculosis transaminase bioa complexed with substrate kapa (see paper)
55% identity, 96% coverage: 8:425/436 of query aligns to 10:426/427 of 4cxqA
- active site: Y18 (= Y16), Y149 (= Y144), E212 (= E211), D246 (= D245), A249 (= A248), K275 (= K274), Y399 (= Y398)
- binding 7-keto-8-aminopelargonic acid: W56 (= W51), Y149 (= Y144), G308 (= G307), T310 (= T309), R392 (= R391)
- binding pyridoxal-5'-phosphate: G116 (= G111), S117 (= S112), Y149 (= Y144), H150 (= H145), G151 (= G146), E212 (= E211), D246 (= D245), I248 (= I247), K275 (= K274), P309 (= P308), T310 (= T309)
4w1vA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a thiazole inhibitor (see paper)
55% identity, 96% coverage: 8:425/436 of query aligns to 10:424/425 of 4w1vA
- active site: Y18 (= Y16), Y147 (= Y144), E210 (= E211), D244 (= D245), A247 (= A248), K273 (= K274), Y397 (= Y398)
- binding dimethyl (2R)-5-(3-fluorophenyl)-1H-pyrrolo[1,2-c][1,3]thiazole-6,7-dicarboxylate 2-oxide: P17 (= P15), Y18 (= Y16), W54 (= W51), M81 (= M78), G83 (= G80), Y147 (= Y144), G306 (= G307), P307 (= P308), T308 (= T309), F392 (= F393)
- binding pyridoxal-5'-phosphate: G114 (= G111), S115 (= S112), Y147 (= Y144), H148 (= H145), E210 (= E211), D244 (= D245), I246 (= I247), K273 (= K274)
4cxrA Mycobaterium tuberculosis transaminase bioa complexed with 1-(1,3- benzothiazol-2-yl)methanamine (see paper)
55% identity, 96% coverage: 8:425/436 of query aligns to 10:424/425 of 4cxrA
- active site: Y18 (= Y16), Y147 (= Y144), E210 (= E211), D244 (= D245), A247 (= A248), K273 (= K274), Y397 (= Y398)
- binding 1-(1,3-benzothiazol-2-yl)methanamine: Y18 (= Y16), W54 (= W51), W55 (= W52), A216 (= A217)
- binding pyridoxal-5'-phosphate: G114 (= G111), S115 (= S112), Y147 (= Y144), H148 (= H145), E210 (= E211), D244 (= D245), I246 (= I247), K273 (= K274), P307 (= P308), T308 (= T309)
1qj3A Crystal structure of 7,8-diaminopelargonic acid synthase in complex with 7-keto-8-aminopelargonic acid (see paper)
56% identity, 95% coverage: 4:417/436 of query aligns to 5:405/416 of 1qj3A
- active site: Y17 (= Y16), Y144 (= Y144), E201 (= E211), D235 (= D245), A238 (= A248), K264 (= K274), Y386 (= Y398)
- binding 7-keto-8-aminopelargonic acid: Y17 (= Y16), W52 (= W51), Y144 (= Y144), K264 (= K274), R379 (= R391), F381 (= F393)
- binding pyridoxal-5'-phosphate: G112 (= G111), S113 (= S112), Y144 (= Y144), H145 (= H145), G146 (= G146), D235 (= D245), I237 (= I247), A238 (= A248), K264 (= K274)
P9WQ81 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase; 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; 7,8-diaminopelargonic acid synthase; DAPAS; Diaminopelargonic acid synthase; EC 2.6.1.62 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
54% identity, 96% coverage: 8:425/436 of query aligns to 17:434/437 of P9WQ81
- Y25 (= Y16) mutation to A: Does not show detectable activity at 335 nm with SAM, even up to concentrations of 3 mM, and shows approximately 70% reduced activity with high concentrations of DAPA (0.5 mM).
- W64 (= W51) binding
- Y157 (= Y144) binding
- K283 (= K274) modified: N6-(pyridoxal phosphate)lysine
- G316 (= G307) binding
5te2A Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a mechanism-based inhibitor (see paper)
54% identity, 96% coverage: 8:425/436 of query aligns to 10:427/428 of 5te2A
- active site: Y18 (= Y16), Y150 (= Y144), E213 (= E211), D247 (= D245), A250 (= A248), K276 (= K274), Y400 (= Y398)
- binding [5-hydroxy-4-({[6-(3-hydroxypropyl)-4-oxo-1,4-dihydropyridin-3-yl]amino}methyl)-6-methylpyridin-3-yl]methyl dihydrogen phosphate: Y18 (= Y16), W57 (= W51), G117 (= G111), S118 (= S112), Y150 (= Y144), H151 (= H145), G152 (= G146), D247 (= D245), I249 (= I247), K276 (= K274), G309 (= G307), P310 (= P308), T311 (= T309)
4xjoA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with an inhibitor optimized from hts lead (see paper)
54% identity, 96% coverage: 8:425/436 of query aligns to 10:427/428 of 4xjoA
- active site: Y18 (= Y16), Y150 (= Y144), E213 (= E211), D247 (= D245), A250 (= A248), K276 (= K274), Y400 (= Y398)
- binding 5-[4-(3-chlorobenzoyl)piperazin-1-yl]-1H-inden-1-one: P17 (= P15), Y18 (= Y16), W57 (= W51), M84 (= M78), G86 (= G80), Y150 (= Y144), G165 (≠ S159), G166 (= G160), A219 (= A217), P310 (= P308), T311 (= T309)
- binding pyridoxal-5'-phosphate: G117 (= G111), S118 (= S112), Y150 (= Y144), H151 (= H145), G152 (= G146), E213 (= E211), D247 (= D245), I249 (= I247), K276 (= K274), P310 (= P308), T311 (= T309)
4xewA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a hts lead compound (see paper)
54% identity, 96% coverage: 8:425/436 of query aligns to 10:427/428 of 4xewA
- active site: Y18 (= Y16), Y150 (= Y144), E213 (= E211), D247 (= D245), A250 (= A248), K276 (= K274), Y400 (= Y398)
- binding 6-(2-fluorophenyl)[1,3]dioxolo[4,5-g]quinolin-8(5H)-one: P17 (= P15), Y18 (= Y16), W57 (= W51), Y150 (= Y144), P310 (= P308), T311 (= T309), R393 (= R391), F395 (= F393)
- binding pyridoxal-5'-phosphate: G117 (= G111), S118 (= S112), Y150 (= Y144), H151 (= H145), G152 (= G146), E213 (= E211), D247 (= D245), I249 (= I247), K276 (= K274), P310 (= P308), T311 (= T309)
4wyfA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a dsf fragment hit (see paper)
54% identity, 96% coverage: 8:425/436 of query aligns to 10:427/428 of 4wyfA
- active site: Y18 (= Y16), Y150 (= Y144), E213 (= E211), D247 (= D245), A250 (= A248), K276 (= K274), Y400 (= Y398)
- binding N-(1-oxo-1H-inden-5-yl)acetamide: M84 (= M78), G86 (= G80), G309 (= G307), P310 (= P308), T311 (= T309)
- binding pyridoxal-5'-phosphate: G117 (= G111), S118 (= S112), Y150 (= Y144), H151 (= H145), G152 (= G146), E213 (= E211), D247 (= D245), I249 (= I247), K276 (= K274), P310 (= P308), T311 (= T309)
4wycA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a thiazole benzamide inhibitor (see paper)
54% identity, 96% coverage: 8:425/436 of query aligns to 10:427/428 of 4wycA
- active site: Y18 (= Y16), Y150 (= Y144), E213 (= E211), D247 (= D245), A250 (= A248), K276 (= K274), Y400 (= Y398)
- binding 4-(1H-imidazol-1-yl)benzamide: P17 (= P15), Y18 (= Y16), W57 (= W51), M84 (= M78), G86 (= G80), Y150 (= Y144), G309 (= G307)
- binding pyridoxal-5'-phosphate: G117 (= G111), S118 (= S112), Y150 (= Y144), H151 (= H145), G152 (= G146), E213 (= E211), D247 (= D245), I249 (= I247), K276 (= K274), P310 (= P308), T311 (= T309)
4mqrA Mycobaterium tuberculosis transaminase bioa complexed with e)-5- hydroxy-4-(((z)-isonicotinoyldiazenyl)methylene)-6-methyl-1,4- dihydropyridin-3-yl)methyl phosphate (see paper)
54% identity, 96% coverage: 8:425/436 of query aligns to 10:427/428 of 4mqrA
- active site: Y18 (= Y16), Y150 (= Y144), E213 (= E211), D247 (= D245), A250 (= A248), K276 (= K274), Y400 (= Y398)
- binding [(4Z)-5-hydroxy-6-methyl-4-{[(E)-(pyridin-4-ylcarbonyl)diazenyl]methylidene}-1,4-dihydropyridin-3-yl]methyl dihydrogen phosphate: Y18 (= Y16), W57 (= W51), G117 (= G111), S118 (= S112), Y150 (= Y144), H151 (= H145), D247 (= D245), I249 (= I247), K276 (= K274), G309 (= G307), P310 (= P308), T311 (= T309)
4mqqA Mycobaterium tuberculosis transaminase bioa complexed with benzo[d]thiazole-2-carbohydrazide (see paper)
54% identity, 96% coverage: 8:425/436 of query aligns to 10:427/428 of 4mqqA
- active site: Y18 (= Y16), Y150 (= Y144), E213 (= E211), D247 (= D245), A250 (= A248), K276 (= K274), Y400 (= Y398)
- binding (4-{[(E)-(1,3-benzothiazol-2-ylcarbonyl)diazenyl]methyl}-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate: Y18 (= Y16), W57 (= W51), W58 (= W52), G117 (= G111), S118 (= S112), Y150 (= Y144), H151 (= H145), D247 (= D245), I249 (= I247), K276 (= K274), P310 (= P308), T311 (= T309)
4mqpA Mycobaterium tuberculosis transaminase bioa complexed with 2- hydrazinylbenzo[d]thiazole (see paper)
54% identity, 96% coverage: 8:425/436 of query aligns to 10:427/428 of 4mqpA
- active site: Y18 (= Y16), Y150 (= Y144), E213 (= E211), D247 (= D245), A250 (= A248), K276 (= K274), Y400 (= Y398)
- binding (4-{[(E)-1,3-benzothiazol-2-yldiazenyl]methyl}-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate: W57 (= W51), W58 (= W52), G117 (= G111), S118 (= S112), Y150 (= Y144), H151 (= H145), E213 (= E211), A219 (= A217), D247 (= D245), I249 (= I247), K276 (= K274), Y400 (= Y398)
5kgtA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with an inhibitor optimized from hts lead: 1-[4-[4-(3-chlorophenyl)carbonylpiperidin-1- yl]phenyl]ethanone (see paper)
54% identity, 96% coverage: 8:425/436 of query aligns to 10:427/429 of 5kgtA
- active site: Y18 (= Y16), Y150 (= Y144), E213 (= E211), D247 (= D245), A250 (= A248), K276 (= K274), Y400 (= Y398)
- binding 1-[4-[4-(3-chlorophenyl)carbonylpiperidin-1-yl]phenyl]ethanone: M84 (= M78), G86 (= G80), G309 (= G307), T311 (= T309)
- binding pyridoxal-5'-phosphate: S116 (= S110), G117 (= G111), S118 (= S112), Y150 (= Y144), H151 (= H145), G152 (= G146), E213 (= E211), D247 (= D245), I249 (= I247), K276 (= K274)
5kgsA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with an inhibitor optimized from hts lead: 5-[4-(1,3-benzodioxol-5-ylcarbonyl)piperazin-1-yl]-2, 3-dihydroinden-1-one (see paper)
54% identity, 96% coverage: 8:425/436 of query aligns to 10:427/429 of 5kgsA
- active site: Y18 (= Y16), Y150 (= Y144), E213 (= E211), D247 (= D245), A250 (= A248), K276 (= K274), Y400 (= Y398)
- binding 5-[4-(1,3-benzodioxol-5-ylcarbonyl)piperazin-1-yl]-2,3-dihydroinden-1-one: P17 (= P15), Y18 (= Y16), W57 (= W51), M84 (= M78), G86 (= G80), Y150 (= Y144), D162 (= D156), G165 (≠ S159), G166 (= G160), P310 (= P308), T311 (= T309), F395 (= F393)
- binding pyridoxal-5'-phosphate: G117 (= G111), S118 (= S112), Y150 (= Y144), H151 (= H145), G152 (= G146), E213 (= E211), D247 (= D245), I249 (= I247), K276 (= K274)
Query Sequence
>6937267 FitnessBrowser__SB2B:6937267
MSIDFEFDRNHLWHPYTSMAAPLPVLGVQSARGCRLTLEDGRELVDGTSSWWSAIHGYGH
PALVSAVQAQAAKLSHVMFGGLTHEPATALGKALLKLVHPTLTRVFYADSGSVAVEVALK
MAMQYWQGRSEPQKHKLLTVLGGYHGDTFAAMSVCDPNSGMHGMFGHLVPKQIFAKAPPA
GFGRSLAEQDLAELRALLQAHHQEIAALIIEPIMQGAGGLRFHSPDYLKSVRALCDEYRV
LLILDEIATGFGRTGKAFAYEHAGITPDLLCLGKALTGGMLSLAATLCTDEVAAGISSSP
AGVFMHGPTFMANPLACAAALASLELFAENRWPAQVANIEAKLTELLETARTLPQVADVR
VLGAVGVIEMVQTVDTAFALMAFAARGVWVRPFGRLIYVMPPYCISDSELGMLTRAMVEV
ASLEGICKTANNPAHG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory