SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 6937493 FitnessBrowser__SB2B:6937493 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

P69783 PTS system glucose-specific EIIA component; EIIA-Glc; EIII-Glc; Glucose-specific phosphotransferase enzyme IIA component from Escherichia coli (strain K12) (see 6 papers)
63% identity, 95% coverage: 1:161/169 of query aligns to 1:161/169 of P69783

query
sites
P69783

M
|
M

G

G

F

L

F
|
F

S

D

R

K

I

L

R

K

R

S

L

L

I

V

S

S

G

D

A

D

P

K

T

K

I

D

E

T

G

G

G

T

I

I

A

E

I

I

L

I

A

A

P

P

V

L

S

S

G

G

E

E

I

I

Y

V

P

N

I

I

E

E

K

D

V

V

P

P

D

D

V

V

F

F

A

A

E

E

K

K

I

I

V

V

G

G

D

D

G

G

I

I

A

A

I

I

A

K

P

P

E

T

G

G

D

N

T

K

I

M

Y

V

A

A

P

P

I

V

D

D

G

G

T

T

I

I

G

G

K

K

I

I

F

F

E

E

T

T

N

N

H
|
H

A

A

F

F

S

S

I

I

E

E

S

S

P

D

Q

S

G

G

L

V

E

E

L

L

F

F

V

V

H
|
H

F

F

G

G

V

I

G

D

T

T

V

V

E

E

L

L

R

K

G

G

N

E

G

G

F

F

K

K

R

R

L

I

A

A

E

E

G

G

Q

Q

Q

R

V

V

K

K

A

V

G

G

D

D

P

T

I

V

L

I

A

E

F

F

D

D

L

L

E

P

Y

L

L

L

R

E

E

E

H

K

A

A

D

K

S

S

L

T

L

L

T

T

P

P

V

V

L

I

A

S

N

N

M

M

E

D

D

E

I

I

K

K

Y

E

L

L

E

I

K

K

S

L

Q

S

G

G

R

S

V

V

E

T

A

V

G

G

K

E

M1 (= M1) modified: Initiator methionine, Removed
F4 (= F4) mutation to W: Same activity as the wild-type.
H76 (= H76) Important for phospho-donor activity; binding ; mutation to Q: Unable to transfer phosphoryl group.
H91 (= H91) active site, Tele-phosphohistidine intermediate; for EIIA activity; binding ; modified: Phosphohistidine; by HPr; mutation to Q: Unable to be phosphorylated by Hpr.

1glcF Cation promoted association (cpa) of a regulatory and target protein is controlled by phosphorylation (see paper)
64% identity, 95% coverage: 2:161/169 of query aligns to 1:153/161 of 1glcF

query
sites
1glcF

G

G

F

L

F

F

S

D

R

K

I

L

R

K

R

S

L

L

I

V

S

S

G

-

A

-

P

-

T

T

I

I

E

E

G

-

I

-

A

-

I

I

L

I

A

A

P

P

V

L

S

S

G

G

E

E

I

I

Y

V

P

N

I

I

E

E

K

D

V

V

P

P

D

D

V

V

F

F

A

A

E

E

K

K

I

I

V

V

G

G

D

D

G

G

I

I

A

A

I

I

A

K

P

P

E

T

G

G

D

N

T

K

I

M

Y

V

A

A

P

P

I

V

D

D

G

G

T

T

I

I

G

G

K

K

I

I

F

F

E

E

T

T

N

N

H
|
H

A

A

F

F

S

S

I

I

E

E

S

S

P

D

Q

S

G

G

L

V

E

E

L

L

F

F

V

V

H
|
H

F

F

G

G

V

I

G

D

T

T

V

V

E

E

L

L

R

K

G

G

N

E

G

G

F

F

K

K

R

R

L

I

A

A

E

E

G

G

Q

Q

Q

R

V

V

K

K

A

V

G

G

D

D

P

T

I

V

L

I

A

E

F

F

D

D

L

L

E

P

Y

L

L

L

R

E

E

E

H

K

A

A

D

K

S

S

L

T

L

L

T

T

P

P

V

V

L

I

A

S

N

N

M

M

E

D

D

E

I

I

K

K

Y

E

L

L

E

I

K

K

S

L

Q

S

G

G

R

S

V

V

E

T

A

V

G

G

K

E

binding zinc ion: H68 (= H76), H83 (= H91)

1o2fA Complex of enzyme iiaglc and iibglc phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
68% identity, 83% coverage: 21:161/169 of query aligns to 2:142/150 of 1o2fA

query
sites
1o2fA

I

I

A

E

I

I

L

I

A

A

P

P

V

L

S

S

G

G

E

E

I

I

Y

V

P

N

I

I

E

E

K

D

V

V

P

P

D

D

V

V

F

F

A

A

E

E

K

K

I

I

V

V

G

G

D

D

G

G

I

I

A

A

I

I

A

K

P

P

E

T

G

G

D

N

T

K

I

M

Y

V

A

A

P

P

I

V

D

D

G

G

T

T

I

I

G

G

K

K

I

I

F

F

E

E

T
|
T

N

N

H
|
H

A

A

F

F

S

S

I

I

E

E

S

S

P

D

Q

S

G

G

L

V

E

E

L

L

F

F

V

V

H
|
H

F

F

G

G

V

I

G
x
D

T

T

V

V

E

E

L

L

R

K

G

G

N

E

G

G

F

F

K

K

R

R

L

I

A

A

E

E

G

G

Q

Q

Q

R

V

V

K

K

A

V

G

G

D

D

P

T

I

V

L

I

A

E

F

F

D

D

L

L

E

P

Y

L

L

L

R

E

E

E

H

K

A

A

D

K

S

S

L

T

L

L

T

T

P

P

V

V

L

I

A

S

N

N

M

M

E

D

D

E

I

I

K

K

Y

E

L

L

E

I

K

K

S

L

Q

S

G

G

R

S

V

V

E

T

A

V

G

G

K

E

active site: T55 (= T74), H57 (= H76), H72 (= H91)
binding phosphite ion: H72 (= H91), D76 (≠ G95)

P09323 PTS system N-acetylglucosamine-specific EIICBA component; EIICBA-Nag; EII-Nag; EC 2.7.1.193 from Escherichia coli (strain K12) (see paper)
46% identity, 86% coverage: 23:168/169 of query aligns to 501:647/648 of P09323

query
sites
P09323

I

L

L

V

A

S

P

P

V

I

S

T

G

G

E

D

I

V

Y

V

P

A

I

L

E

D

K

Q

V

V

P

P

D

D

V

E

V

A

F

F

A

A

E

S

K

K

I

A

V

V

G

G

D

D

G

G

I

V

A

A

I

V

A

K

P

P

E

T

G

D

D

K

T

I

I

V

Y

V

A

S

P

P

I

A

D

A

G

G

T

T

I

I

G

V

K

K

I

I

F

F

E

N

T

T

N

N

H

H

A

A

F

F

S

C

I

L

E

E

S

T

P

E

Q

K

G

G

L

A

E

E

L

I

F

V

V

V

H

H

F

M

G

G

V

I

G

D

T

T

V

V

E

A

L

L

R

E

G

G

N

K

G

G

F

F

K

K

R

R

L

L

A

V

E

E

G

G

Q

A

Q

Q

V

V

K

S

A

A

G

G

D

Q

P

P

I

I

L

L

A

E

F

M

D

D

L

L

E

D

Y

Y

L

L

R

N

E

A

H

N

A

A

D

R

S

S

L

M

L

I

T

S

P

P

V

V

L

C

A

S

N

N

M

I

E

D

D

D

I

F

K

S

Y

G

L

L

-

I

E

I

K

K

S

A

Q

Q

G

G

R

H

V

I

E

V

A

A

G

G

K

Q

D

T

V

P

I

L

F

Y

T

E

V

I

E

K

Sites not aligning to the query:

1 modified: N-formylmethionine

Query Sequence

>6937493 FitnessBrowser__SB2B:6937493
MGFFSRIRRLISGAPTIEGGIAILAPVSGEIYPIEKVPDVVFAEKIVGDGIAIAPEGDTI
YAPIDGTIGKIFETNHAFSIESPQGLELFVHFGVGTVELRGNGFKRLAEEGQQVKAGDPI
LAFDLEYLREHADSLLTPVVLANMEDIKYLEKSQGRVEAGKDVIFTVEI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory