SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 6937867 FitnessBrowser__SB2B:6937867 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

8sadA Crystal structure of cystathionine beta lyase from klebsiella aerogenes, plp/malonate complex (c2 form)
52% identity, 98% coverage: 1:391/401 of query aligns to 4:397/398 of 8sadA

query
sites
8sadA

M

M

S

A

D

D

K

K

P

H

M

L

K

D

T

T

A

A

T

-

K

-

I

L

V

V

S

N

L

A

G

G

R

R

E

R

K

K

K

K

Y

Y

S

T

K

Q

G

G

V

S

I

V

N

N

P

S

P

V

V

I

F

Q

R

R

A

A

S

S

T

S

I

L

V

V

F

F

D

D

T

T

M

V

E

E

E

A

M

K

R

K

H

H

A

A

A

T

K

R

N

N

K

R

H

A

N

K

G

G

E

E

M

L

F

F

Y

Y

G

G

R

R

R

R

G

G

T

T

P

L

T

T

H

H

F

F

G

S

F

L

Q

Q

A

E

A

A

I

M

A

C

E

E

L

L

E

E

G

G

G

G

A

A

G

G

T

C

A

A

L

L

Y

F

P

P

S
x
C

G
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G

A
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A

A

A

A

A

I

V

S

A

A

N

S

T

L

I

L

L

A

A

F

F

L

V

Q

E

A

Q

G

G

D

D

H

H

L

V

L

L

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N

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A

Y
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Y

E

E

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P

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Q

D

D

L

F

C

C

D

T

K

K

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I

L

L

K

A

G

K

Y

L

G

G

I

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E

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T

T

T

G

Y

W

Y

F

D

D

P

P

L

L

I

I

G

G

A

A

G

D

I

I

E

A

A

N

L

L

I

I

R

Q

P

P

N

N

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T

R

K

V

V

L

V

F

F

V

L

E

E

S

S

P

P

G

G

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S

I

I

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T

M

M

E

E

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V

Q

H

D

D

V

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P

T

A

L

I

A

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-

A

-

A

-

V

-

R

R

R

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A

H

P

E

E

H

-

G

-

L

A

V

I

V

I

I

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L

I

D
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D

N

N

T

T

W

W

A

A

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A

P

G

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F

R

K

P

A

F

L

E

D

L

F

G

G

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I

D

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I

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A

A

A

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K

Y

Y

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H

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M
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M

M

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G

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A

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R

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C

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C

E

E

H

N

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A

Y

Y

L

L

L

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G

G

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Q

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T

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A

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D

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A

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Y

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R

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G

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L

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R

T

T

L

L

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G

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R

R

M

L

A

R

Q

Q

H

H

E

H

K

E

N

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L

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V

A

A

N

E

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W

L

L

K

A

T

Q

R

H

P

P

E

Q

V

V

D

A

H

R

L

V

R

N

H

H

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P

A

A

F

L

E

P

E

G

C

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K

G

G

H

H

E

E

F

F

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K

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R

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D

F

F

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G

G

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N

S

G

G

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L

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F

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V

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L

K

N

R

K

G

R

T

L

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T

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D

A

A

-

E

V

L

T

A

A

A

M

Y

V

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E

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M

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S

H

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K

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M

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G

G

G

G

F

F

E

E

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I

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x
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x
A

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E

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P

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D

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A

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G

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F

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A

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I

binding magnesium ion: A359 (vs. gap), R362 (= R356), A365 (≠ T359)
binding pyridoxal-5'-phosphate: C88 (≠ S87), G89 (= G88), A90 (= A89), Y114 (= Y113), D188 (= D185), A210 (= A207), T212 (= T209), K213 (= K210), M222 (= M219), W343 (= W339)

8sabA Crystal structure of cystathionine beta lyase from klebsiella aerogenes, plp adduct with alanine (c2 form)
52% identity, 95% coverage: 10:391/401 of query aligns to 5:391/392 of 8sabA

query
sites
8sabA

T

T

K

A

I

L

V

V

S
x
N

L

A

G

G

R
|
R

E
x
R

K
|
K

K

K

Y

Y

S
x
T

K

Q

G

G

V

S

I

V

N

N

P

S

P

V

V

I

F

Q

R

R

A

A

S

S

T

S

I

L

V

V

F

F

D

D

T

T

M

V

E

E

E

A

M

K

R

K

H

H

A

A

A

T

K

R

N

N

K

R

H

A

N

K

G

G

E

E

M

L

F

F

Y

Y

G

G

R

R

R

R

G

G

T

T

P

L

T

T

H

H

F

F

G

S

F

L

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Q

A

E

A

A

I

M

A

C

E

E

L

L

E

E

G

G

G

G

A

A

G

G

T

C

A

A

L

L

Y

F

P

P

S
x
C

G
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G

A
|
A

A

A

A

A

I

V

S

A

A

N

S

T

L

I

L

L

A

A

F

F

L

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Q

E

A

Q

G

G

D

D

H

H

L

V

L

L

M

M

V

T

D

N

S

T

V

A

Y
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Y

E

E

P

P

T

S

R

Q

D

D

L

F

C

C

D

T

K

K

V

I

L

L

K

A

G

K

Y

L

G

G

I

V

E

T

T

T

T

G

Y

W

Y

F

D

D

P

P

L

L

I

I

G

G

A

A

G

D

I

I

E

A

A

N

L

L

I

I

R

Q

P

P

N

N

T

T

R

K

V

V

L

V

F

F

V

L

E

E

S

S

P

P

G

G

S

S

I

I

T

T

M

M

E

E

V

V

Q

H

D

D

V

V

P

P

T

A

L

I

A

V

-

A

-

A

-

V

-

R

R

R

I

V

A

A

H

P

E

E

H

-

G

-

L

A

V

I

V

I

I

M

L

I

D
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D

N

N

T

T

W

W

A

A

S

A

P

G

I

V

N

L

S

F

R

K

P

A

F

L

E

D

L

F

G

G

V

I

D

D

V

I

S

S

I

I

Q

Q

A
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A

A

A

T
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T

K
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K

Y

Y

I

L

V

I

G

G

H

H

S

S

D

D

V

G

M
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M

M

I

G

G

T

T

A

A

T

V

A

A

S

N

P

A

Q

R

Y

C

W

W

D

E

K

Q

L

L

R

C

E

E

H

N

S

A

Y

Y

L

L

L

M

G

G

Q

Q

T

M

T

I

S

D

P

A

D

D

D

T

V

A

Y

Y

L

M

A

T

A

S

R

R

G

G

L

L

R

R

T

T

L

L

G

G

V

V

R

R

M

L

A

R

Q

Q

H

H

E

H

K

E

N

S

A

S

L

L

A

R

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V

A

A

N

E

W

W

L

L

K

A

T

Q

R

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P

P

E

Q

V

V

D

A

H

R

L

V

R

N

H

H

P

P

A

A

F

L

E

P

E

G

C

S

P

K

G

G

H

H

E

E

F

F

F

W

K

K

R

R

D

D

F

F

S

S

G

G

S

S

N

S

G

G

L

L

F

F

S

S

F

F

V

V

L

L

K

N

R

K

G

R

T

L

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T

D

D

A

A

-

E

V

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T

A

A

A

M

Y

V

L

E

D

G

N

M

F

S

S

H
x
L

F

F

K

S

M

M

G

A

F
x
Y

S
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S

W
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W

G

G

G

G

F

F

E
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E

S

S

L

L

I

I

L

L

G

A

V

N

T

Q

G

P

I

E

H

H

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I

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A

N

A

I

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A

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V

D

D

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S

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G

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T

L

L

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R

V

L

H

H

I

I

G

G

L

L

E

E

D

N

P

V

E

D

D

D

L

L

I

L

E

A

D

D

L

L

S

A

A

A

G

G

F

F

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A

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R

F

I

binding lysine: N9 (≠ S14), R12 (= R17), R13 (≠ E18), K14 (= K19), T17 (≠ S22), L330 (≠ H332), E341 (= E343)
binding pyridoxal-5'-phosphate: C82 (≠ S87), G83 (= G88), A84 (= A89), Y108 (= Y113), D182 (= D185), A204 (= A207), T206 (= T209), K207 (= K210), M216 (= M219), W337 (= W339)
binding alanyl-pyridoxal-5'-phosphate: C82 (≠ S87), G83 (= G88), A84 (= A89), Y108 (= Y113), D182 (= D185), A204 (= A207), T206 (= T209), K207 (= K210), M216 (= M219), Y335 (≠ F337), S336 (= S338), W337 (= W339), R369 (= R369)

8u99A Crystal structure of cystathionine beta lyase from klebsiella aerogenes (plp-serine adduct)
52% identity, 95% coverage: 10:391/401 of query aligns to 4:390/391 of 8u99A

query
sites
8u99A

T

T

K

A

I

L

V

V

S

N

L

A

G

G

R

R

E

R

K

K

K

K

Y

Y

S

T

K

Q

G

G

V

S

I

V

N

N

P

S

P

V

V

I

F

Q

R

R

A

A

S

S

T

S

I

L

V

V

F

F

D

D

T

T

M

V

E

E

E

A

M

K

R

K

H

H

A

A

A

T

K

R

N

N

K

R

H

A

N

K

G

G

E

E

M

L

F

F

Y

Y

G

G

R

R

R

R

G

G

T

T

P

L

T

T

H

H

F

F

G

S

F

L

Q

Q

A

E

A

A

I

M

A

C

E

E

L

L

E

E

G

G

G

G

A

A

G

G

T

C

A

A

L

L

Y

F

P

P

S
x
C

G
|
G

A
|
A

A

A

A

A

I

V

S

A

A

N

S

T

L

I

L

L

A

A

F

F

L

V

Q

E

A

Q

G

G

D

D

H

H

L

V

L

L

M

M

V

T

D

N

S

T

V

A

Y
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Y

E

E

P

P

T

S

R

Q

D

D

L

F

C

C

D

T

K

K

V

I

L

L

K

A

G

K

Y

L

G

G

I

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E

T

T

T

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G

Y

W

Y

F

D

D

P

P

L

L

I

I

G

G

A

A

G

D

I

I

E

A

A

N

L

L

I

I

R

Q

P

P

N

N

T

T

R

K

V

V

L

V

F

F

V

L

E

E

S

S

P

P

G

G

S

S

I

I

T

T

M

M

E

E

V

V

Q

H

D

D

V

V

P

P

T

A

L

I

A

V

-

A

-

A

-

V

-

R

R

R

I

V

A

A

H

P

E

E

H

-

G

-

L

A

V

I

V

I

I

M

L

I

D
|
D

N

N

T

T

W

W

A

A

S

A

P

G

I

V

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L

S

F

R

K

P

A

F

L

E

D

L

F

G

G

V

I

D

D

V

I

S

S

I

I

Q

Q

A

A

A

A

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T

K
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K

Y

Y

I

L

V

I

G

G

H

H

S

S

D

D

V

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M
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M

M

I

G

G

T

T

A

A

T

V

A

A

S

N

P

A

Q

R

Y

C

W

W

D

E

K

Q

L

L

R

C

E

E

H

N

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A

Y

Y

L

L

L

M

G

G

Q

Q

T

M

T

I

S

D

P

A

D

D

D

T

V

A

Y

Y

L

M

A

T

A

S

R

R

G

G

L

L

R

R

T

T

L

L

G

G

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V

R

R

M

L

A

R

Q

Q

H

H

E

H

K

E

N

S

A

S

L

L

A

R

V

V

A

A

N

E

W

W

L

L

K

A

T

Q

R

H

P

P

E

Q

V

V

D

A

H

R

L

V

R

N

H

H

P

P

A

A

F

L

E

P

E

G

C

S

P

K

G

G

H

H

E

E

F

F

F

W

K

K

R

R

D

D

F

F

S

S

G

G

S

S

N

S

G

G

L

L

F

F

S

S

F

F

V

V

L

L

K

N

R

K

G

R

T

L

V

T

D

D

A

A

-

E

V

L

T

A

A

A

M

Y

V

L

E

D

G

N

M

F

S

S

H

L

F

F

K

S

M

M

G

A

F
x
Y

S
|
S

W
|
W

G

G

G

G

F

F

E

E

S

S

L

L

I

I

L

L

G

A

V

N

T

Q

G

P

I

E

H

H

-

I

-

A

N

A

I

I

R

R

S

P

A

E

T

A

R

E

W

V

D

D

S

F

S

S

K

G

P

T

L

L

I

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R
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R

V

L

H

H

I

I

G

G

L

L

E

E

D

N

P

V

E

D

D

D

L

L

I

L

E

A

D

D

L

L

S

A

A

A

G

G

F

F

V

A

R

R

F

I

binding pyridoxal-5'-phosphate: C81 (≠ S87), G82 (= G88), A83 (= A89), Y107 (= Y113), D181 (= D185), T205 (= T209), K206 (= K210), M215 (= M219), W336 (= W339)
binding serine: Y107 (= Y113), K206 (= K210), Y334 (≠ F337), S335 (= S338), W336 (= W339), R368 (= R369)

8u98A Crystal structure of cystathionine beta lyase from klebsiella aerogenes (plp-glycine adduct)
52% identity, 95% coverage: 10:391/401 of query aligns to 4:390/391 of 8u98A

query
sites
8u98A

T

T

K

A

I

L

V

V

S

N

L

A

G

G

R

R

E

R

K

K

K

K

Y

Y

S

T

K

Q

G

G

V

S

I

V

N

N

P

S

P

V

V

I

F

Q

R

R

A

A

S

S

T

S

I

L

V

V

F

F

D

D

T

T

M

V

E

E

E

A

M

K

R

K

H

H

A

A

A

T

K

R

N

N

K

R

H

A

N

K

G

G

E

E

M

L

F

F

Y
|
Y

G

G

R
|
R

R

R

G

G

T

T

P

L

T

T

H

H

F

F

G

S

F

L

Q

Q

A

E

A

A

I

M

A

C

E

E

L

L

E

E

G

G

G

G

A

A

G

G

T

C

A

A

L

L

Y

F

P

P

S
x
C

G
|
G

A
|
A

A

A

A

A

I

V

S

A

A

N

S

T

L

I

L

L

A

A

F

F

L

V

Q

E

A

Q

G

G

D

D

H

H

L

V

L

L

M

M

V

T

D

N

S

T

V

A

Y
|
Y

E

E

P

P

T

S

R

Q

D

D

L

F

C

C

D

T

K

K

V

I

L

L

K

A

G

K

Y

L

G

G

I

V

E

T

T

T

T

G

Y

W

Y

F

D

D

P

P

L

L

I

I

G

G

A

A

G

D

I

I

E

A

A

N

L

L

I

I

R

Q

P

P

N

N

T

T

R

K

V

V

L

V

F

F

V

L

E

E

S

S

P

P

G

G

S

S

I

I

T

T

M

M

E

E

V

V

Q

H

D

D

V

V

P

P

T

A

L

I

A

V

-

A

-

A

-

V

-

R

R

R

I

V

A

A

H

P

E

E

H

-

G

-

L

A

V

I

V

I

I

M

L

I

D
|
D

N

N

T

T

W

W

A

A

S

A

P

G

I

V

N

L

S

F

R

K

P

A

F

L

E

D

L

F

G

G

V

I

D

D

V

I

S

S

I

I

Q

Q

A
|
A

A

A

T
|
T

K
|
K

Y

Y

I

L

V

I

G

G

H

H

S

S

D

D

V

G

M
|
M

M

I

G

G

T

T

A

A

T

V

A

A

S

N

P

A

Q

R

Y

C

W

W

D

E

K

Q

L

L

R

C

E

E

H

N

S

A

Y

Y

L

L

L

M

G

G

Q

Q

T

M

T

I

S

D

P

A

D

D

D

T

V

A

Y

Y

L

M

A

T

A

S

R

R

G

G

L

L

R

R

T

T

L

L

G

G

V

V

R

R

M

L

A

R

Q

Q

H

H

E

H

K

E

N

S

A

S

L

L

A

R

V

V

A

A

N

E

W

W

L

L

K

A

T

Q

R

H

P

P

E

Q

V

V

D

A

H

R

L

V

R

N

H

H

P

P

A

A

F

L

E

P

E

G

C

S

P

K

G

G

H

H

E

E

F

F

F

W

K

K

R

R

D

D

F

F

S

S

G

G

S

S

N

S

G

G

L

L

F

F

S

S

F

F

V

V

L

L

K

N

R

K

G

R

T

L

V

T

D

D

A

A

-

E

V

L

T

A

A

A

M

Y

V

L

E

D

G

N

M

F

S

S

H

L

F

F

K

S

M

M

G

A

F
x
Y

S
|
S

W
|
W

G

G

G

G

F

F

E

E

S

S

L

L

I

I

L

L

G

A

V

N

T

Q

G

P

I

E

H

H

-

I

-

A

N

A

I

I

R

R

S

P

A

E

T

A

R

E

W

V

D

D

S

F

S

S

K

G

P

T

L

L

I

I

R
|
R

V

L

H

H

I

I

G

G

L

L

E

E

D

N

P

V

E

D

D

D

L

L

I

L

E

A

D

D

L

L

S

A

A

A

G

G

F

F

V

A

R

R

F

I

binding glycine: Y107 (= Y113), K206 (= K210), Y334 (≠ F337), S335 (= S338), W336 (= W339), R368 (= R369)
binding pyridoxal-5'-phosphate: Y52 (= Y58), R54 (= R60), C81 (≠ S87), G82 (= G88), A83 (= A89), Y107 (= Y113), D181 (= D185), A203 (= A207), T205 (= T209), K206 (= K210), M215 (= M219), W336 (= W339)

8sa9A Crystal structure of cystathionine beta lyase from klebsiella aerogenes, plp-oxamate adduct (c2 form)
52% identity, 95% coverage: 10:391/401 of query aligns to 4:390/391 of 8sa9A

query
sites
8sa9A

T

T

K

A

I

L

V

V

S

N

L

A

G

G

R

R

E

R

K

K

K

K

Y

Y

S

T

K

Q

G

G

V

S

I

V

N

N

P

S

P

V

V

I

F

Q

R

R

A

A

S

S

T

S

I

L

V

V

F

F

D

D

T

T

M

V

E

E

E

A

M

K

R

K

H

H

A

A

A

T

K

R

N

N

K

R

H

A

N

K

G

G

E

E

M

L

F

F

Y

Y

G

G

R

R

R

R

G

G

T

T

P

L

T

T

H

H

F

F

G

S

F

L

Q

Q

A

E

A

A

I

M

A

C

E

E

L

L

E

E

G

G

G

G

A

A

G

G

T

C

A

A

L

L

Y

F

P

P

S
x
C

G
|
G

A
|
A

A

A

A

A

I

V

S

A

A

N

S

T

L

I

L

L

A

A

F

F

L

V

Q

E

A

Q

G

G

D

D

H

H

L

V

L

L

M

M

V

T

D

N

S

T

V

A

Y
|
Y

E

E

P

P

T

S

R

Q

D

D

L

F

C

C

D

T

K

K

V

I

L

L

K

A

G

K

Y

L

G

G

I

V

E

T

T

T

T

G

Y

W

Y

F

D

D

P

P

L

L

I

I

G

G

A

A

G

D

I

I

E

A

A

N

L

L

I

I

R

Q

P

P

N

N

T

T

R

K

V

V

L

V

F

F

V

L

E

E

S

S

P

P

G

G

S

S

I

I

T

T

M

M

E

E

V

V

Q

H

D

D

V

V

P

P

T

A

L

I

A

V

-

A

-

A

-

V

-

R

R

R

I

V

A

A

H

P

E

E

H

-

G

-

L

A

V

I

V

I

I

M

L

I

D
|
D

N

N

T

T

W

W

A

A

S

A

P

G

I

V

N

L

S

F

R

K

P

A

F

L

E

D

L

F

G

G

V

I

D

D

V

I

S

S

I

I

Q

Q

A
|
A

A

A

T
|
T

K
|
K

Y

Y

I

L

V

I

G

G

H

H

S

S

D

D

V

G

M
|
M

M

I

G

G

T

T

A

A

T

V

A

A

S

N

P

A

Q

R

Y

C

W

W

D

E

K

Q

L

L

R

C

E

E

H

N

S

A

Y

Y

L

L

L

M

G

G

Q

Q

T

M

T

I

S

D

P

A

D

D

D

T

V

A

Y

Y

L

M

A

T

A

S

R

R

G

G

L

L

R

R

T

T

L

L

G

G

V

V

R

R

M

L

A

R

Q

Q

H

H

E

H

K

E

N

S

A

S

L

L

A

R

V

V

A

A

N

E

W

W

L

L

K

A

T

Q

R

H

P

P

E

Q

V

V

D

A

H

R

L

V

R

N

H

H

P

P

A

A

F

L

E

P

E

G

C

S

P

K

G

G

H

H

E

E

F

F

F

W

K

K

R

R

D

D

F

F

S

S

G

G

S

S

N

S

G

G

L

L

F

F

S

S

F

F

V

V

L

L

K

N

R

K

G

R

T

L

V

T

D

D

A

A

-

E

V

L

T

A

A

A

M

Y

V

L

E

D

G

N

M

F

S

S

H

L

F

F

K

S

M

M

G

A

F

Y

S
|
S

W
|
W

G

G

G

G

F

F

E

E

S

S

L

L

I

I

L

L

G

A

V

N

T

Q

G

P

I

E

H

H

-

I

-

A

N

A

I

I

R

R

S

P

A

E

T

A

R

E

W

V

D

D

S

F

S

S

K

G

P

T

L

L

I

I

R
|
R

V

L

H

H

I

I

G

G

L

L

E

E

D

N

P

V

E

D

D

D

L

L

I

L

E

A

D

D

L

L

S

A

A

A

G

G

F

F

V

A

R

R

F

I

binding pyridoxal-5'-phosphate: C81 (≠ S87), G82 (= G88), A83 (= A89), Y107 (= Y113), D181 (= D185), A203 (= A207), T205 (= T209), K206 (= K210), M215 (= M219), W336 (= W339)
binding [({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino](oxo)acetic acid: C81 (≠ S87), G82 (= G88), A83 (= A89), Y107 (= Y113), D181 (= D185), A203 (= A207), T205 (= T209), K206 (= K210), M215 (= M219), S335 (= S338), W336 (= W339), R368 (= R369)

P06721 Cystathionine beta-lyase MetC; CBL; CL; Beta-cystathionase MetC; Cysteine desulfhydrase MetC; CD; Cysteine lyase MetC; Cysteine-S-conjugate beta-lyase MetC; EC 4.4.1.13; EC 4.4.1.28 from Escherichia coli (strain K12) (see 2 papers)
51% identity, 98% coverage: 1:391/401 of query aligns to 1:394/395 of P06721

query
sites
P06721

M

M

S

A

D

D

K

K

P

-

M

-

K

K

T

L

A

D

T

T

K

Q

I

L

V

V

S

N

L

A

G

G

R

R

E

S

K

K

K

K

Y

Y

S

T

K

L

G

G

V

A

I

V

N

N

P

S

P

V

V

I

F

Q

R

R

A

A

S

S

T

S

I

L

V

V

F

F

D

D

T

S

M

V

E

E

E

A

M

K

R

K

H

H

A

A

A

T

K

R

N

N

K

R

H

A

N

N

G

G

E

E

M

L

F

F

Y

Y

G

G

R

R

R

R

G

G

T

T

P

L

T

T

H

H

F

F

G

S

F

L

Q

Q

A

Q

A

A

I

M

A

C

E

E

L

L

E

E

G

G

G

G

A

A

G

G

T

C

A

V

L

L

Y

F

P

P

S

C

G

G

A

A

A

A

A

A

I

V

S

A

A

N

S

S

L

I

L

L

A

A

F

F

L

I

Q

E

A

Q

G

G

D

D

H

H

L

V

L

L

M

M

V

T

D

N

S

T

V

A

Y

Y

E

E

P

P

T

S

R

Q

D

D

L

F

C

C

D

S

K

K

V

I

L

L

K

S

G

K

Y

L

G

G

I

V

E

T

T

T

T

S

Y

W

Y

F

D

D

P

P

L

L

I

I

G

G

A

A

G

D

I

I

E

V

A

K

L

H

I

L

R

Q

P

P

N

N

T

T

R

K

V

I

L

V

F

F

V

L

E

E

S

S

P

P

G

G

S

S

I

I

T

T

M

M

E

E

V

V

Q

H

D

D

V

V

P

P

T

A

L

I

A

V

R

A

I

A

A

V

H

R

E

S

-

V

-

V

H

P

G

D

L

A

V

I

V

I

I

M

L

I

D

D

N

N

T

T

W

W

A

A

S

A

P

G

I

V

N

L

S

F

R

K

P

A

F

L

E

D

L

F

G

G

V

I

D

D

V

V

S

S

I

I

Q

Q

A

A

A

A

T

T

K
|
K

Y

Y

I

L

V

V

G

G

H

H

S

S

D

D

V

A

M

M

M

I

G

G

T

T

A

A

T

V

A

C

S

N

P

A

Q

R

Y

C

W

W

D

E

K

Q

L

L

R

R

E

E

H

N

S

A

Y

Y

L

L

L

M

G

G

Q

Q

T

M

T

V

S

D

P

A

D

D

D

T

V

A

Y

Y

L

I

A

T

A

S

R

R

G

G

L

L

R

R

T

T

L

L

G

G

V

V

R

R

M

L

A

R

Q

Q

H

H

E

H

K

E

N

S

A

S

L

L

A

K

V

V

A

A

N

E

W

W

L

L

K

A

T

E

R

H

P

P

E

Q

V

V

D

A

H

R

L

V

R

N

H

H

P

P

A

A

F

L

E

P

E

G

C

S

P

K

G

G

H

H

E

E

F

F

F

W

K

K

R

R

D

D

F

F

S

T

G

G

S

S

N

S

G

G

L

L

F

F

S

S

F

F

V

V

L

L

K

K

R

K

G

K

-

L

T

N

V

N

D

E

A

E

V

L

T

A

A

N

M

Y

V

L

E

D

G

N

M

F

S

S

H

L

F

F

K

S

M

M

G

A

F

Y

S

S

W

W

G

G

G

G

F

Y

E

E

S

S

L

L

I

I

L

L

G

A

V

N

T

Q

G

P

I

E

H

H

-

I

-

A

N

A

I

I

R

R

S

P

A

Q

T

G

R

E

W

I

D

D

S

F

S

S

K

G

P

T

L

L

I

I

R

R

V

L

H

H

I

I

G

G

L

L

E

E

D

D

P

V

E

D

D

D

L

L

I

I

E

A

D

D

L

L

S

D

A

A

G

G

F

F

V

A

R

R

F

I

K210 (= K210) modified: N6-(pyridoxal phosphate)lysine

1cl1B Cystathionine beta-lyase (cbl) from escherichia coli (see paper)
51% identity, 96% coverage: 7:391/401 of query aligns to 2:391/392 of 1cl1B

query
sites
1cl1B

K

K

T

L

A

D

T

T

K

Q

I

L

V

V

S

N

L

A

G

G

R

R

E

S

K

K

K

K

Y

Y

S

T

K

L

G

G

V

A

I

V

N

N

P

S

P

V

V

I

F

Q

R

R

A

A

S

S

T

S

I

L

V

V

F

F

D

D

T

S

M

V

E

E

E

A

M

K

R

K

H

H

A

A

A

T

K

R

N

N

K

R

H

A

N

N

G

G

E

E

M

L

F

F

Y

Y

G

G

R
|
R

R

R

G

G

T

T

P

L

T

T

H

H

F

F

G

S

F

L

Q

Q

A

Q

A

A

I

M

A

C

E

E

L

L

E

E

G

G

G

G

A

A

G

G

T

C

A

V

L

L

Y

F

P

P

S

C

G

G

A

A

A

A

A

A

I

V

S

A

A

N

S

S

L

I

L

L

A

A

F

F

L

I

Q

E

A

Q

G

G

D

D

H

H

L

V

L

L

M

M

V

T

D

N

S

T

V

A

Y
|
Y

E

E

P

P

T

S

R

Q

D

D

L

F

C

C

D

S

K

K

V

I

L

L

K

S

G

K

Y

L

G

G

I

V

E

T

T

T

T

S

Y

W

Y

F

D

D

P

P

L

L

I

I

G

G

A

A

G

D

I

I

E

V

A

K

L

H

I

L

R

Q

P

P

N

N

T

T

R

K

V

I

L

V

F

F

V

L

E

E

S

S

P

P

G

G

S

S

I

I

T

T

M

M

E

E

V

V

Q

H

D

D

V

V

P

P

T

A

L

I

A

V

R

A

I

A

A

V

H

R

E

S

-

V

-

V

H

P

G

D

L

A

V

I

V

I

I

M

L

I

D
|
D

N

N

T

T

W

W

A

A

S

A

P

G

I

V

N

L

S

F

R

K

P

A

F

L

E

D

L

F

G

G

V

I

D

D

V

V

S

S

I

I

Q

Q

A

A

A

A

T

T

K
|
K

Y

Y

I

L

V

V

G

G

H

H

S

S

D

D

V

A

M

M

M

I

G

G

T

T

A

A

T

V

A

C

S

N

P

A

Q

R

Y

C

W

W

D

E

K

Q

L

L

R

R

E

E

H

N

S

A

Y

Y

L

L

L

M

G

G

Q

Q

T

M

T

V

S

D

P

A

D

D

D

T

V

A

Y

Y

L

I

A

T

A

S

R

R

G

G

L

L

R

R

T

T

L

L

G

G

V

V

R

R

M

L

A

R

Q

Q

H

H

E

H

K

E

N

S

A

S

L

L

A

K

V

V

A

A

N

E

W

W

L

L

K

A

T

E

R

H

P

P

E

Q

V

V

D

A

H

R

L

V

R

N

H

H

P

P

A

A

F

L

E

P

E

G

C

S

P

K

G

G

H

H

E

E

F

F

F

W

K

K

R

R

D

D

F

F

S

T

G

G

S

S

N

S

G

G

L

L

F

F

S

S

F

F

V

V

L

L

K

K

R

K

G

K

-

L

T

N

V

N

D

E

A

E

V

L

T

A

A

N

M

Y

V

L

E

D

G

N

M

F

S

S

H

L

F

F

K

S

M

M

G

A

F

Y

S
|
S

W
|
W

G

G

G

G

F

Y

E

E

S

S

L

L

I

I

L

L

G

A

V

N

T

Q

G

P

I

E

H

H

-

I

-

A

N

A

I

I

R

R

S

P

A

Q

T

G

R

E

W

I

D

D

S

F

S

S

K

G

P

T

L

L

I

I

R
|
R

V

L

H

H

I

I

G

G

L

L

E

E

D

D

P

V

E

D

D

D

L

L

I

I

E

A

D

D

L

L

S

D

A

A

G

G

F

F

V

A

R

R

F

I

active site: R55 (= R60), Y108 (= Y113), D182 (= D185), K207 (= K210)
binding bicarbonate ion: Y108 (= Y113), K207 (= K210), S336 (= S338), W337 (= W339), R369 (= R369)

2gqnA Cystathionine beta-lyase (cbl) from escherichia coli in complex with n-hydrazinocarbonylmethyl-2-nitro-benzamide (see paper)
51% identity, 96% coverage: 7:391/401 of query aligns to 1:390/391 of 2gqnA

query
sites
2gqnA

K

K

T

L

A

D

T

T

K

Q

I

L

V

V

S

N

L

A

G

G

R

R

E

S

K

K

K

K

Y

Y

S

T

K

L

G

G

V

A

I

V

N

N

P

S

P

V

V

I

F

Q

R

R

A

A

S

S

T

S

I

L

V

V

F

F

D

D

T

S

M

V

E

E

E

A

M

K

R

K

H

H

A

A

A

T

K

R

N

N

K

R

H

A

N

N

G

G

E

E

M

L

F

F

Y

Y

G

G

R
|
R

R

R

G

G

T

T

P

L

T

T

H

H

F

F

G

S

F

L

Q

Q

A

Q

A

A

I

M

A

C

E

E

L

L

E

E

G

G

G

G

A

A

G

G

T

C

A

V

L

L

Y

F

P

P

S
x
C

G
|
G

A
|
A

A

A

A

A

I

V

S

A

A

N

S

S

L

I

L

L

A

A

F

F

L

I

Q

E

A

Q

G

G

D

D

H

H

L

V

L

L

M

M

V

T

D

N

S

T

V

A

Y
|
Y

E
|
E

P

P

T

S

R

Q

D

D

L

F

C

C

D

S

K

K

V

I

L

L

K

S

G

K

Y

L

G

G

I

V

E

T

T

T

T

S

Y

W

Y

F

D

D

P

P

L

L

I

I

G

G

A

A

G

D

I

I

E

V

A

K

L

H

I

L

R

Q

P

P

N

N

T

T

R

K

V

I

L

V

F

F

V

L

E

E

S

S

P

P

G

G

S

S

I

I

T

T

M

M

E

E

V

V

Q

H

D

D

V

V

P

P

T

A

L

I

A

V

R

A

I

A

A

V

H

R

E

S

-

V

-

V

H

P

G

D

L

A

V

I

V

I

I

M

L

I

D
|
D

N

N

T

T

W

W

A

A

S

A

P

G

I

V

N

L

S

F

R

K

P

A

F

L

E

D

L

F

G

G

V

I

D

D

V

V

S

S

I

I

Q

Q

A
|
A

A

A

T
|
T

K
|
K

Y

Y

I

L

V

V

G

G

H

H

S

S

D

D

V

A

M
|
M

M

I

G

G

T

T

A

A

T

V

A

C

S

N

P

A

Q

R

Y

C

W

W

D

E

K

Q

L

L

R

R

E

E

H

N

S

A

Y

Y

L

L

L

M

G

G

Q

Q

T

M

T

V

S

D

P

A

D

D

D

T

V

A

Y

Y

L

I

A

T

A

S

R

R

G

G

L

L

R

R

T

T

L

L

G

G

V

V

R

R

M

L

A

R

Q

Q

H

H

E

H

K

E

N

S

A

S

L

L

A

K

V

V

A

A

N

E

W

W

L

L

K

A

T

E

R

H

P

P

E

Q

V

V

D

A

H

R

L

V

R

N

H

H

P

P

A

A

F

L

E

P

E

G

C

S

P

K

G

G

H

H

E

E

F

F

F

W

K

K

R

R

D

D

F

F

S

T

G

G

S

S

N

S

G

G

L

L

F

F

S

S

F

F

V

V

L

L

K

K

R

K

G

K

-

L

T

N

V

N

D

E

A

E

V

L

T

A

A

N

M

Y

V

L

E

D

G

N

M

F

S

S

H

L

F

F

K

S

M

M

G

A

F
x
Y

S
|
S

W
|
W

G

G

G

G

F

Y

E

E

S

S

L

L

I

I

L

L

G

A

V

N

T

Q

G

P

I

E

H

H

-

I

-

A

N

A

I

I

R

R

S

P

A

Q

T

G

R

E

W

I

D

D

S

F

S

S

K

G

P

T

L

L

I

I

R
|
R

V

L

H

H

I

I

G

G

L

L

E

E

D

D

P

V

E

D

D

D

L

L

I

I

E

A

D

D

L

L

S

D

A

A

G

G

F

F

V

A

R

R

F

I

active site: R54 (= R60), Y107 (= Y113), D181 (= D185), K206 (= K210)
binding (5-hydroxy-6-methyl-4-((2-(2-(2-nitrobenzamido)acetyl)hydrazinyl)methyl)pyridin-3-yl)methyl dihydrogen phosphate: C81 (≠ S87), G82 (= G88), A83 (= A89), Y107 (= Y113), E108 (= E114), D181 (= D185), A203 (= A207), T205 (= T209), K206 (= K210), M215 (= M219), Y334 (≠ F337), S335 (= S338), W336 (= W339), R368 (= R369)

2fq6A Cystathionine beta-lyase (cbl) from escherichia coli in complex with n-hydrazinocarbonylmethyl-2-trifluoromethyl-benzamide (see paper)
51% identity, 96% coverage: 7:391/401 of query aligns to 1:390/391 of 2fq6A

query
sites
2fq6A

K

K

T

L

A

D

T

T

K

Q

I

L

V

V

S

N

L

A

G

G

R

R

E

S

K

K

K

K

Y

Y

S

T

K

L

G

G

V

A

I

V

N

N

P

S

P

V

V

I

F

Q

R

R

A

A

S

S

T

S

I

L

V

V

F

F

D

D

T

S

M

V

E

E

E

A

M

K

R

K

H

H

A

A

A

T

K

R

N

N

K

R

H

A

N

N

G

G

E

E

M

L

F

F

Y

Y

G

G

R
|
R

R

R

G

G

T

T

P

L

T

T

H

H

F

F

G

S

F

L

Q

Q

A

Q

A

A

I

M

A

C

E

E

L

L

E

E

G

G

G

G

A

A

G

G

T

C

A

V

L

L

Y

F

P

P

S
x
C

G
|
G

A
|
A

A

A

A

A

I

V

S

A

A

N

S

S

L

I

L

L

A

A

F

F

L

I

Q

E

A

Q

G

G

D

D

H

H

L

V

L

L

M

M

V

T

D

N

S

T

V

A

Y
|
Y

E

E

P
|
P

T

S

R

Q

D

D

L

F

C

C

D

S

K

K

V

I

L

L

K

S

G

K

Y

L

G

G

I

V

E

T

T

T

T

S

Y

W

Y

F

D

D

P

P

L

L

I

I

G

G

A

A

G

D

I

I

E

V

A

K

L

H

I

L

R

Q

P

P

N

N

T

T

R

K

V

I

L

V

F

F

V

L

E

E

S

S

P

P

G

G

S

S

I

I

T

T

M

M

E

E

V

V

Q

H

D

D

V

V

P

P

T

A

L

I

A

V

R

A

I

A

A

V

H

R

E

S

-

V

-

V

H

P

G

D

L

A

V

I

V

I

I

M

L

I

D
|
D

N

N

T

T

W

W

A

A

S

A

P

G

I

V

N

L

S

F

R

K

P

A

F

L

E

D

L

F

G

G

V

I

D

D

V

V

S

S

I

I

Q

Q

A
|
A

A

A

T
|
T

K
|
K

Y

Y

I

L

V

V

G

G

H

H

S

S

D

D

V

A

M
|
M

M

I

G

G

T

T

A

A

T

V

A

C

S

N

P

A

Q

R

Y

C

W

W

D

E

K

Q

L

L

R

R

E

E

H

N

S

A

Y

Y

L

L

L

M

G

G

Q

Q

T

M

T

V

S

D

P

A

D

D

D

T

V

A

Y

Y

L

I

A

T

A

S

R

R

G

G

L

L

R

R

T

T

L

L

G

G

V

V

R

R

M

L

A

R

Q

Q

H

H

E

H

K

E

N

S

A

S

L

L

A

K

V

V

A

A

N

E

W

W

L

L

K

A

T

E

R

H

P

P

E

Q

V

V

D

A

H

R

L

V

R

N

H

H

P

P

A

A

F

L

E

P

E

G

C

S

P

K

G

G

H

H

E

E

F

F

F

W

K

K

R

R

D

D

F

F

S

T

G

G

S

S

N

S

G

G

L

L

F

F

S

S

F

F

V

V

L

L

K

K

R

K

G

K

-

L

T

N

V

N

D

E

A

E

V

L

T

A

A

N

M

Y

V

L

E

D

G

N

M

F

S

S

H

L

F

F

K

S

M

M

G

A

F
x
Y

S
|
S

W
|
W

G

G

G

G

F

Y

E

E

S

S

L

L

I

I

L

L

G

A

V

N

T

Q

G

P

I

E

H

H

-

I

-

A

N

A

I

I

R

R

S

P

A

Q

T

G

R

E

W

I

D

D

S

F

S

S

K

G

P

T

L

L

I

I

R
|
R

V

L

H

H

I

I

G

G

L

L

E

E

D

D

P

V

E

D

D

D

L

L

I

I

E

A

D

D

L

L

S

D

A

A

G

G

F

F

V

A

R

R

F

I

active site: R54 (= R60), Y107 (= Y113), D181 (= D185), K206 (= K210)
binding phosphoric acid mono-(5-hydroxy-6-methyl-4-{[2-(2-trifluoromethyl-benzoylamino)-acetyl]-hydrazonomethyl}-pyridin-3-ylmethyl)ester: C81 (≠ S87), G82 (= G88), A83 (= A89), Y107 (= Y113), P109 (= P115), D181 (= D185), A203 (= A207), T205 (= T209), K206 (= K210), M215 (= M219), Y334 (≠ F337), S335 (= S338), W336 (= W339), R368 (= R369)

1cl2A Cystathionine beta-lyase (cbl) from escherichia coli in complex with aminoethoxyvinylglycine (see paper)
51% identity, 96% coverage: 7:391/401 of query aligns to 1:390/391 of 1cl2A

query
sites
1cl2A

K

K

T

L

A

D

T

T

K

Q

I

L

V

V

S

N

L

A

G

G

R

R

E

S

K

K

K

K

Y

Y

S

T

K

L

G

G

V

A

I

V

N

N

P

S

P

V

V

I

F

Q

R

R

A

A

S

S

T

S

I

L

V

V

F

F

D

D

T

S

M

V

E

E

E

A

M

K

R

K

H

H

A

A

A

T

K

R

N

N

K

R

H

A

N

N

G

G

E

E

M

L

F

F

Y

Y

G

G

R
|
R

R

R

G

G

T

T

P

L

T

T

H

H

F

F

G

S

F

L

Q

Q

A

Q

A

A

I

M

A

C

E

E

L

L

E

E

G

G

G

G

A

A

G

G

T

C

A

V

L

L

Y

F

P

P

S
x
C

G
|
G

A
|
A

A

A

A

A

I

V

S

A

A

N

S

S

L

I

L

L

A

A

F

F

L

I

Q

E

A

Q

G

G

D

D

H

H

L

V

L

L

M

M

V

T

D

N

S

T

V

A

Y
|
Y

E

E

P

P

T

S

R

Q

D

D

L

F

C

C

D

S

K

K

V

I

L

L

K

S

G

K

Y

L

G

G

I

V

E

T

T

T

T

S

Y

W

Y

F

D

D

P

P

L

L

I

I

G

G

A

A

G

D

I

I

E

V

A

K

L

H

I

L

R

Q

P

P

N

N

T

T

R

K

V

I

L

V

F

F

V

L

E

E

S

S

P

P

G

G

S

S

I

I

T

T

M

M

E

E

V

V

Q

H

D

D

V

V

P

P

T

A

L

I

A

V

R

A

I

A

A

V

H

R

E

S

-

V

-

V

H

P

G

D

L

A

V

I

V

I

I

M

L

I

D
|
D

N

N

T

T

W

W

A

A

S

A

P

G

I

V

N

L

S

F

R

K

P

A

F

L

E

D

L

F

G

G

V

I

D

D

V

V

S

S

I

I

Q

Q

A
|
A

A

A

T
|
T

K
|
K

Y

Y

I

L

V

V

G

G

H

H

S

S

D

D

V

A

M
|
M

M

I

G

G

T

T

A

A

T

V

A

C

S

N

P

A

Q

R

Y

C

W

W

D

E

K

Q

L

L

R

R

E

E

H

N

S

A

Y

Y

L

L

L

M

G

G

Q

Q

T

M

T

V

S

D

P

A

D

D

D

T

V

A

Y

Y

L

I

A

T

A

S

R

R

G

G

L

L

R

R

T

T

L

L

G

G

V

V

R

R

M

L

A

R

Q

Q

H

H

E

H

K

E

N

S

A

S

L

L

A

K

V

V

A

A

N

E

W

W

L

L

K

A

T

E

R

H

P

P

E

Q

V

V

D

A

H

R

L

V

R

N

H

H

P

P

A

A

F

L

E

P

E

G

C

S

P

K

G

G

H

H

E

E

F

F

F

W

K

K

R

R

D

D

F

F

S

T

G

G

S

S

N

S

G

G

L

L

F

F

S

S

F

F

V

V

L

L

K

K

R

K

G

K

-

L

T

N

V

N

D

E

A

E

V

L

T

A

A

N

M

Y

V

L

E

D

G

N

M

F

S

S

H

L

F

F

K

S

M

M

G

A

F
x
Y

S
|
S

W
|
W

G

G

G

G

F

Y

E

E

S

S

L

L

I

I

L

L

G

A

V

N

T

Q

G

P

I

E

H

H

-

I

-

A

N

A

I

I

R

R

S

P

A

Q

T

G

R

E

W

I

D

D

S

F

S

S

K

G

P

T

L

L

I

I

R
|
R

V

L

H

H

I

I

G

G

L

L

E

E

D

D

P

V

E

D

D

D

L

L

I

I

E

A

D

D

L

L

S

D

A

A

G

G

F

F

V

A

R

R

F

I

active site: R54 (= R60), Y107 (= Y113), D181 (= D185), K206 (= K210)
binding (2E,3E)-4-(2-aminoethoxy)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]but-3-enoic acid: C81 (≠ S87), G82 (= G88), A83 (= A89), Y107 (= Y113), D181 (= D185), A203 (= A207), T205 (= T209), K206 (= K210), M215 (= M219), Y334 (≠ F337), S335 (= S338), W336 (= W339), R368 (= R369)

4itxA P113s mutant of e. Coli cystathionine beta-lyase metc inhibited by reaction with l-ala-p (see paper)
51% identity, 96% coverage: 7:391/401 of query aligns to 1:390/391 of 4itxA

query
sites
4itxA

K

K

T

L

A

D

T

T

K

Q

I

L

V

V

S

N

L

A

G

G

R

R

E

S

K

K

K

K

Y

Y

S

T

K

L

G

G

V

A

I

V

N

N

P

S

P

V

V

I

F

Q

R

R

A

A

S

S

T

S

I

L

V

V

F

F

D

D

T

S

M

V

E

E

E

A

M

K

R

K

H

H

A

A

A

T

K

R

N

N

K

R

H

A

N

N

G

G

E

E

M

L

F

F

Y

Y

G

G

R
|
R

R

R

G

G

T

T

P

L

T

T

H

H

F

F

G

S

F

L

Q

Q

A

Q

A

A

I

M

A

C

E

E

L

L

E

E

G

G

G

G

A

A

G

G

T

C

A

V

L

L

Y

F

P

P

S
x
C

G
|
G

A
|
A

A

A

A

A

I

V

S

A

A

N

S

S

L

I

L

L

A

A

F

F

L

I

Q

E

A

Q

G

G

D

D

H

H

L

V

L

L

M

M

V

T

D

N

S

T

V

A

Y
|
Y

E

E

P

S

T

S

R

Q

D

D

L

F

C

C

D

S

K

K

V

I

L

L

K

S

G

K

Y

L

G

G

I

V

E

T

T

T

T

S

Y

W

Y

F

D

D

P

P

L

L

I

I

G

G

A

A

G

D

I

I

E

V

A

K

L

H

I

L

R

Q

P

P

N

N

T

T

R

K

V

I

L

V

F

F

V

L

E

E

S

S

P

P

G

G

S

S

I

I

T

T

M

M

E

E

V

V

Q

H

D

D

V

V

P

P

T

A

L

I

A

V

R

A

I

A

A

V

H

R

E

S

-

V

-

V

H

P

G

D

L

A

V

I

V

I

I

M

L

I

D
|
D

N

N

T

T

W

W

A

A

S

A

P

G

I

V

N

L

S

F

R

K

P

A

F

L

E

D

L

F

G

G

V

I

D

D

V

V

S

S

I

I

Q

Q

A
|
A

A

A

T
|
T

K
|
K

Y

Y

I

L

V

V

G

G

H

H

S

S

D

D

V

A

M
|
M

M

I

G

G

T

T

A

A

T

V

A

C

S

N

P

A

Q

R

Y

C

W

W

D

E

K

Q

L

L

R

R

E

E

H

N

S

A

Y

Y

L

L

L

M

G

G

Q

Q

T

M

T

V

S

D

P

A

D

D

D

T

V

A

Y

Y

L

I

A

T

A

S

R

R

G

G

L

L

R

R

T

T

L

L

G

G

V

V

R

R

M

L

A

R

Q

Q

H

H

E

H

K

E

N

S

A

S

L

L

A

K

V

V

A

A

N

E

W

W

L

L

K

A

T

E

R

H

P

P

E

Q

V

V

D

A

H

R

L

V

R

N

H

H

P

P

A

A

F

L

E

P

E

G

C

S

P

K

G

G

H

H

E

E

F

F

F

W

K

K

R

R

D

D

F

F

S

T

G

G

S

S

N

S

G

G

L

L

F

F

S

S

F

F

V

V

L

L

K

K

R

K

G

K

-

L

T

N

V

N

D

E

A

E

V

L

T

A

A

N

M

Y

V

L

E

D

G

N

M

F

S

S

H

L

F

F

K

S

M

M

G

A

F
x
Y

S
|
S

W
|
W

G

G

G

G

F

Y

E

E

S

S

L

L

I

I

L

L

G

A

V

N

T

Q

G

P

I

E

H

H

-

I

-

A

N

A

I

I

R

R

S

P

A

Q

T

G

R

E

W

I

D

D

S

F

S

S

K

G

P

T

L

L

I

I

R
|
R

V

L

H

H

I

I

G

G

L

L

E

E

D

D

P

V

E

D

D

D

L

L

I

I

E

A

D

D

L

L

S

D

A

A

G

G

F

F

V

A

R

R

F

I

active site: R54 (= R60), Y107 (= Y113), D181 (= D185), K206 (= K210)
binding {1-[(3-hydroxy-methyl-5-phosphonooxy-methyl-pyridin-4-ylmethyl)-amino]-ethyl}-phosphonic acid: C81 (≠ S87), G82 (= G88), A83 (= A89), Y107 (= Y113), D181 (= D185), A203 (= A207), T205 (= T209), K206 (= K210), M215 (= M219), Y334 (≠ F337), S335 (= S338), W336 (= W339), R368 (= R369)

4l0oH Structure determination of cystathionine gamma-synthase from helicobacter pylori
34% identity, 96% coverage: 6:391/401 of query aligns to 1:373/373 of 4l0oH

query
sites
4l0oH

M

M

K

R

T

M

A

Q

T

T

K

K

I

L

V

I

S

H

L

G

G

G

R

I

E

S

K

E

K

D

Y

A

S

T

K

T

G

G

V

A

I

V

N

S

P

V

P

P

V

I

F

Y

R

Q

A

T

S

S

T

T

I

Y

V

R

F

Q

D

D

T

G

M

Y

E

E

E

-

M

-

R

-

H

-

A

-

A

-

K

-

N

-

K

-

H

-

N

-

G

-

E

-

M

-

F

-

Y
|
Y

G

S

R
|
R

R

S

G

G

T

N

P

P

T

T

H

R

F

F

G

A

F

L

Q

E

A

E

A

L

I

I

A

A

E

D

L

L

E

E

G

G

G

G

A

V

G

K

T

G

A

F

L

A

Y

F

P

A

S
|
S

G
|
G

A
x
L

A

A

A

G

I

I

S

H

A

A

S

-

L

V

L

F

A

S

F

L

L

L

Q

Q

A

S

G

G

D

D

H

H

L

V

L

L

M

L

V

G

D

D

S

D

V

V

Y
|
Y

E

G

P

G

T

T

R

F

D

R

L

L