SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 6937998 FitnessBrowser__SB2B:6937998 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1kgzB Crystal structure analysis of the anthranilate phosphoribosyltransferase from erwinia carotovora (current name, pectobacterium carotovorum) (see paper)
53% identity, 96% coverage: 8:337/344 of query aligns to 5:329/330 of 1kgzB

query
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1kgzB

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binding manganese (ii) ion: S92 (= S97), D222 (= D229), E223 (= E230), E223 (= E230)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V79 (= V84), G82 (= G87), G83 (= G88), N90 (= N95), I91 (= I96), S92 (= S97), T93 (= T98), K108 (= K113), S118 (= S125)

5nofA Anthranilate phosphoribosyltransferase from thermococcus kodakaraensis (see paper)
43% identity, 97% coverage: 7:341/344 of query aligns to 2:325/325 of 5nofA

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binding zinc ion: E48 (≠ D55), D78 (= D89), E198 (= E210), D217 (= D229), E218 (= E230)

4yi7A Anthranilate bound at active site of anthranilate phosphoribosyl transferase from acinetobacter (anprt; trpd)
41% identity, 96% coverage: 4:332/344 of query aligns to 3:319/331 of 4yi7A

query
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4yi7A

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active site: V82 (= V84)
binding 2-aminobenzoic acid: V82 (= V84), G83 (= G85), H110 (= H114), R154 (= R171), G167 (= G184)

1zxyA Anthranilate phosphoribosyltransferase from sulfolobus solfataricus in complex with prpp and magnesium (see paper)
34% identity, 98% coverage: 4:339/344 of query aligns to 2:330/344 of 1zxyA

query
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1zxyA

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D

N

S

A

A

L

I

-

K

I

I

T

V

A

R

A

A

I

F

L

L

K

-

G

-

E

-

G

G

K

K

A

D

A

E

H

H

-

V

R

A

D

E

A

F

V

I

A

K

L

I

N

N

A

T

G

A

C

V

A

A

L

L

Y

F

V

A

A

L

G

D

L

R

A

V

D

G

T

D

P

F

Q

R

A

E

G

G

F

Y

K

E

L

Y

A

A

I

-

D

D

T

H

L

L

A

I

S

E

G

-

K

K

A

S

Y

L

E

D

K

K

L

L

I

N

G

E

L

I

A

I

A

S

M

M

S

N

binding magnesium ion: D223 (= D229), E224 (= E230)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: A78 (≠ V84), G79 (= G85), D83 (= D89), T87 (= T93), N89 (= N95), S91 (= S97), T92 (= T98), K106 (= K113), S118 (= S125), D223 (= D229), E224 (= E230)

2gvqD Anthranilate phosphoribosyl-transferase (trpd) from s. Solfataricus in complex with anthranilate (see paper)
34% identity, 98% coverage: 4:339/344 of query aligns to 2:330/345 of 2gvqD

query
sites
2gvqD

D

N

V

I

R

N

Q

E

L

I

L

L

D

K

K

K

T

L

Y

I

G

N

G

K

N

S

P

D

L

L

S

E

R

I

E

N

E

E

A

A

K

E

C

E

L

L

F

A

A

K

S

A

L

I

V

I

R

R

G

G

E

E

L

V

D

P

E

E

V

I

T

L

I

V

A

S

A

A

L

I

L

L

M

V

A

A

L

L

K

R

V

M

R

K

G

G

E

E

T

S

I

K

D

N

E

E

I

I

S

V

G

G

A

F

A

A

D

R

A

A

M

M

R

R

E

E

A

L

A

A

N

I

S

K

F

I

P

D

R

V

P

P

A

-

L

-

D

-

G

-

G

N

I

A

L

I

D

D

I

T

V

A

G

G

T

T

G

G

D

D

G

G

F

L

N

G

T

T

I

V

N

N

I

V

S

S

T

T

T

A

A

S

T

A

F

I

V

L

A

L

A

S

A

L

A

V

G

N

A

P

N

-

V

V

A

A

K

K

H
|
H

G
|
G

N
|
N

R

R

S

A

V

V

S

S

S

G

K

K

S

S

G

G

S

S

S

A

D

D

L

V

L

L

G

E

Q

A

F

L

G

G

I

Y

E

N

L

I

T

I

M

V

A

P

P

P

Q

E

T

R

A

A

A

K

N

E

C

L

L

V

E

N

A

K

L

T

G

N

I

F

C

V

F

F

L

L

F

F

A

A

P
x
Q

H

Y

Y

Y

H
|
H

G

P

G

A

V
x
M

K

K

H

N

A

V

V

A

P

N

V

V

R
|
R

Q

K

K

T

L

L

A

G

T

I

R

R

T

T

L

I

F

F

N

N

V

I

L

L

G
|
G

P
|
P

L

L

I

T

N

N

P

P

S

A

H

N

P

A

D

K

F

Y

M

Q

L

L

L

M

G

G

V

V

Y

F

S

S

E

K

T

D

L

H

V

L

R

D

P

L

I

L

A

S

E

K

V

S

L

A

C

Y

A

E

L

L

G

D

V

F

K

N

R

K

A

I

M

I

V

L

V

V

H

Y

G

G

E

E

-

P

G

G

L

I

D

D

E

E

V

V

A

S

L

P

H

I

G

G

T

N

T

T

Q

F

V

M

C

K

E

I

L

V

R

S

N

K

G

R

E

G

L

I

V

E

D

E

Y

V

S

K

L

L

S

N

P

V

K

T

D

D

F

F

G

G

L

I

E

S

P

P

V

I

Q

P

V

I

T

E

E

K

L

L

E

I

G

V

G

N

S

S

P

A

E

E

D

D

N

S

A

A

L

I

-

K

I

I

T

V

A

R

A

A

I

F

L

L

K

-

G

-

E

-

G

G

K

K

A

D

A

E

H

H

-

V

R

A

D

E

A

F

V

I

A

K

L

I

N

N

A

T

G

A

C

V

A

A

L

L

Y

F

V

A

A

L

G

D

L

R

A

V

D

G

T

D

P

F

Q

R

A

E

G

G

F

Y

K

E

L

Y

A

A

I

-

D

D

T

H

L

L

A

I

S

E

G

-

K

K

A

S

Y

L

E

D

K

K

L

L

I

N

G

E

L

I

A

I

A

S

M

M

S

N

binding 2-aminobenzoic acid: H107 (= H114), G108 (= G115), N109 (= N116), N109 (= N116), Q151 (≠ P158), H154 (= H161), M157 (≠ V164), R164 (= R171), G177 (= G184), P178 (= P185)

P50384 Anthranilate phosphoribosyltransferase; EC 2.4.2.18 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see 2 papers)
34% identity, 98% coverage: 4:339/344 of query aligns to 2:330/345 of P50384

query
sites
P50384

D

N

V

I

R

N

Q

E

L

I

L

L

D

K

K

K

T

L

Y

I

G

N

G

K

N

S

P

D

L

L

S

E

R

I

E

N

E

E

A

A

K

E

C

E

L

L

F

A

A

K

S

A

L

I

V

I

R

R

G

G

E

E

L

V

D

P

E

E

V

I

T

L

I

V

A

S

A

A

L

I

L

L

M

V

A

A

L

L

K

R

V

M

R

K

G

G

E

E

T

S

I

K

D

N

E

E

I

I

S

V

G

G

A

F

A

A

D

R

A

A

M

M

R

R

E

E

A

L

A

A

N

I

S

K

F

I

P

D

R

V

P

P

A

-

L

-

D

-

G

-

G

N

I

A

L

I

D

D

I

T

V

A

G

G

T

T

G

G

D

D

G

G

F

L

N

G

T
|
T

I

V

N

N

I

V

S

S

T

T

T

A

A

S

T

A

F

I

V

L

A

L

A

S

A

L

A

V

G

N

A

P

N

-

V

V

A

A

K
|
K

H
|
H

G

G

N

N

R

R

S

A

V

V

S

S

S

G

K

K

S

S

G

G

S
|
S

S

A

D

D

L

V

L

L

G

E

Q

A

F

L

G

G

I

Y

E

N

L

I

T

I

M

V

A

P

P

P

Q

E

T

R

A

A

A

K

N

E

C

L

L

V

E

N

A

K

L

T

G

N

I

F

C

V

F

F

L

L

F

F

A

A

P

Q

H

Y

Y

Y

H
|
H

G

P

G

A

V

M

K

K

H

N

A

V

V

A

P

N

V

V

R
|
R

Q

K

K

T

L

L

A

G

T

I

R

R

T

T

L

I

F

F

N

N

V

I

L

L

G

G

P

P

L

L

I

T

N

N

P

P

S

A

H

N

P

A

D

K

F

Y

M

Q

L

L

L

M

G

G

V

V

Y

F

S

S

E

K

T

D

L

H

V

L

R

D

P

L

I

L

A

S

E

K

V

S

L

A

C

Y

A

E

L

L

G

D

V

F

K

N

R

K

A

I

M

I

V

L

V

V

H

Y

G

G

E

E

-

P

G

G

L

I

D
|
D

E
|
E

V

V

A

S

L

P

H

I

G

G

T

N

T

T

Q

F

V

M

C

K

E

I

L

V

R

S

N

K

G

R

E

G

L

I

V

E

D

E

Y

V

S

K

L

L

S

N

P

V

K

T

D

D

F

F

G

G

L

I

E

S

P

P

V

I

Q

P

V

I

T

E

E

K

L

L

E

I

G

V

G

N

S

S

P

A

E

E

D

D

N

S

A

A

L

I

-

K

I

I

T

V

A

R

A

A

I

F

L

L

K

-

G

-

E

-

G

G

K

K

A

D

A

E

H

H

-

V

R

A

D

E

A

F

V

I

A

K

L

I

N

N

A

T

G

A

C

V

A

A

L

L

Y

F

V

A

A

L

G

D

L

R

A

V

D

G

T

D

P

F

Q

R

A

E

G

G

F

Y

K

E

L

Y

A

A

I

-

D

D

T

H

L

L

A

I

S

E

G

-

K

K

A

S

Y

L

E

D

K

K

L

L

I

N

G

E

L

I

A

I

A

S

M

M

S

N

T87 (= T93) binding
K106 (= K113) mutation to Q: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency dedreases only 10-fold.
H107 (= H114) mutation to A: Limited effect on either affinity for anthranilate and catalytic efficiency. 300-fold decrease of the affinity for anthranilate, whereas catalytic efficiency remains nearly unchanged; when associated with A-178.
S118 (= S125) binding
H154 (= H161) mutation to A: Limited effect on either affinity for anthranilate and catalytic efficiency.
R164 (= R171) mutation to A: Strong decrease of the affinity for anthranilate, although only a moderate 7-fold decrease in catalytic efficiency.
D223 (= D229) mutation to N: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency is unchanged.
E224 (= E230) mutation to Q: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency is unchanged.

1gxbA Anthranilate phosphoribosyltransferase in complex with pyrophosphate and magnesium (see paper)
33% identity, 98% coverage: 4:339/344 of query aligns to 2:326/339 of 1gxbA

query
sites
1gxbA

D

N

V

I

R

N

Q

E

L

I

L

L

D

K

K

K

T

L

Y

I

G

N

G

K

N

S

P

D

L

L

S

E

R

I

E

N

E

E

A

A

K

E

C

E

L

L

F

A

A

K

S

A

L

I

V

I

R

R

G

G

E

E

L

V

D

P

E

E

V

I

T

L

I

V

A

S

A

A

L

I

L

L

M

V

A

A

L

L

K

R

V

M

R

K

G

G

E

E

T

S

I

K

D

N

E

E

I

I

S

V

G

G

A

F

A

A

D

R

A

A

M

M

R

R

E

E

A

L

A

A

N

I

S

K

F

I

P

D

R

V

P

P

A

-

L

-

D

-

G

-

I

-

L

-

D

N

I

A

V

I

G

D

T

T

G

A

G

G

D

-

G

G

F

L

N

G

T

T

I

V

N
|
N

I

V

S
|
S

T
|
T

T

A

A

S

T

A

F

I

V

L

A

L

A

S

A

L

A

V

G

N

A

P

N

-

V

V

A

A

K
|
K

H

H

G

G

N

N

R

R

S

A

V

V

S

S

S

G

K

K

S

S

G

G

S
|
S

S

A

D

D

L

V

L

L

G

E

Q

A

F

L

G

G

I

Y

E

N

L

I

T

I

M

V

A

P

P

P

Q

E

T

R

A

A

A

K

N

E

C

L

L

V

E

N

A

K

L

T

G

N

I

F

C

V

F

F

L

L

F

F

A

A

P

Q

H

Y

Y

Y

H

H

G

P

G

A

V

M

K

K

H

N

A

V

V

A

P

N

V

V

R

R

Q

K

K

T

L

L

A

G

T

I

R

R

T

T

L

I

F

F

N

N

V

I

L

L

G

G

P

P

L

L

I

T

N

N

P

P

S

A

H

N

P

A

D

K

F

Y

M

Q

L

L

L

M

G

G

V

V

Y

F

S

S

E

K

T

D

L

H

V

L

R

D

P

L

I

L

A

S

E

K

V

S

L

A

C

Y

A

E

L

L

G

D

V

F

K

N

R

K

A

I

M

I

V

L

V

V

H

Y

G

G

E

E

-

P

G

G

L

I

D

D

E
|
E

V

V

A

S

L

P

H

I

G

G

T

N

T

T

Q

F

V

M

C

K

E

I

L

V

R

S

N

K

G

R

E

G

L

I

V

E

D

E

Y

V

S

K

L

L

S

N

P

V

K

T

D

D

F

F

G

G

L

I

E

S

P

P

V

I

Q

P

V

I

T

E

E

K

L

L

E

I

G

V

G

N

S

S

P

A

E

E

D

D

N

S

A

A

L

I

-

K

I

I

T

V

A

R

A

A

I

F

L

L

K

-

G

-

E

-

G

G

K

K

A

D

A

E

H

H

-

V

R

A

D

E

A

F

V

I

A

K

L

I

N

N

A

T

G

A

C

V

A

A

L

L

Y

F

V

A

A

L

G

D

L

R

A

V

D

G

T

D

P

F

Q

R

A

E

G

G

F

Y

K

E

L

Y

A

A

I

-

D

D

T

H

L

L

A

I

S

E

G

-

K

K

A

S

Y

L

E

D

K

K

L

L

I

N

G

E

L

I

A

I

A

S

M

M

S

N

binding magnesium ion: S87 (= S97), E220 (= E230)
binding pyrophosphate 2-: N85 (= N95), S87 (= S97), T88 (= T98), K102 (= K113), S114 (= S125)

4zojA Methylsulfanyl-containing inhibitor bound in the active site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
35% identity, 97% coverage: 7:341/344 of query aligns to 5:341/341 of 4zojA

query
sites
4zojA

Q

Q

L

I

L

L

D

G

K

R

T

L

Y

T

G

D

G

N

N

R

P

D

L

L

S

A

R

R

E

G

E

Q

A

A

K

A

C

W

L

A

F

M

A

D

S

Q

L

I

V

M

R

T

G

G

E

N

L

A

D

R

E

P

V

A

T

Q

I

I

A

A

L

F

L

A

M

V

A

A

L

M

K

T

V

M

R

K

G

A

E

P

T

T

I

A

D

D

E

E

I

V

S

G

G

E

A

L

A

A

D

G

A

V

M

M

R

L

E

S

A

H

A

A

N

H

S

P

F

L

P

P

R

A

P

D

A

T

L

V

D

P

G

D

I

A

L

V

D

D

I

V

V
|
V

G
|
G

T

T

G
|
G

D

D

G

G

F

V

N

N

T

T

I

V

N
|
N

I

L

S
|
S

T
|
T

T

M

A

A

T

A

F

I

V

V

A

V

A

A

G

G

A

V

N

P

V

V

A

V

K
|
K

H

H

G

G

N
|
N

R
|
R

S
x
A

V
x
A

S

S

S
|
S

K

L

S

S

G

G

S

G

S

A

D

D

L

T

L

L

G

E

Q

A

F

L

G

G

I

V

E

R

L

I

T

D

M

L

A

G

P

P

Q

D

T

L

A

V

A

A

N

R

C

S

L

L

E

A

A

E

L

V

G

G

I

I

C

G

F

F

L

C

F

F

A
|
A

P
|
P

H

R

Y

F

H
|
H

G

P

G

S

V
x
Y

K

R

H

H

A

A

V

A

P

A

V

V

R
|
R

Q

R

K

E

L

I

A

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G
|
G

P

P

L

L

I

T

N

N

P

P

S

A

H

R

P

P

D

R

F

A

M

G

L

L

L

I

G

G

V

C

Y

A

S

F

E

A

T

D

L

L

V

A

R

E

P

V

I

M

A

A

E

G

V

V

L

F

C

A

A

A

L

-

G

-

V

-

K

R

R

R

A

S

-

S

-

V

M

L

V

V

H

H

G

G

-

D

E

D

G

G

L

L

D
|
D

E
|
E

V

L

A

T

L

T

H

T

G

T

T

T

T

S

Q

T

V

I

C

W

E

R

L

V

R

A

N

A

G

G

E

S

L

V

V

D

D

K

Y

L

S

T

L

F

S

D

P

P

K

A

D

G

F

F

G

G

L

F

E

A

P

R

V

A

Q

Q

V

L

T

D

E

Q

L

L

E

A

G

G

S

D

P

A

E

Q

D

A

N

N

A

A

L

A

I

A

T

V

A

R

A

A

I

V

L

L

K

G

G

G

E

-

G

A

K

R

A

G

A

P

H

V

R

R

D

D

A

A

V

V

A

V

L

L

N

N

A

A

G

A

C

G

A

A

L

I

Y

V

V

A

-

H

A

A

G

G

L

L

A

E

D

W

T

L

P

P

-

A

-

W

Q

E

A

E

G

G

F

L

K

R

L

R

A

A

I

S

D

A

T

A

L

I

A

D

S

T

G

G

K

A

A

A

Y

E

E

Q

K

L

L

L

I

A

G

R

L

W

A

V

A

R

M

F

S

G

Q

R

Q

Q

active site: V82 (= V84)
binding 4-methyl-2-{[2-methyl-6-(methylsulfanyl)phenyl]amino}benzoic acid: N114 (= N116), A155 (= A157), P156 (= P158), H159 (= H161), Y162 (≠ V164), R169 (= R171), G182 (= G184)
binding magnesium ion: S95 (= S97), D227 (= D229), E228 (= E230), E228 (= E230)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G85), G85 (= G87), G86 (= G88), N93 (= N95), S95 (= S97), T96 (= T98), K111 (= K113), R115 (= R117), A116 (≠ S118), A117 (≠ V119), S119 (= S121)

4zokA Methylsulfonyl-containing inhibitor bound in the substrate capture site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
35% identity, 97% coverage: 7:341/344 of query aligns to 5:342/342 of 4zokA

query
sites
4zokA

Q

Q

L

I

L

L

D

G

K

R

T

L

Y

T

G

D

G

N

N

R

P

D

L

L

S

A

R

R

E

G

E

Q

A

A

K

A

C

W

L

A

F

M

A

D

S

Q

L

I

V

M

R

T

G

G

E

N

L

A

D

R

E

P

V

A

T

Q

I

I

A

A

L

F

L

A

M

V

A

A

L

M

K

T

V

M

R

K

G

A

E

P

T

T

I

A

D

D

E

E

I

V

S

G

G

E

A

L

A

A

D

G

A

V

M

M

R

L

E

S

A

H

A

A

N

H

S

P

F

L

P

P

R

A

P

D

A

T

L

V

D

P

G

D

I

A

L

V

D

D

I

V

V
|
V

G

G

T

T

G

G

G
|
G

D

D

G

G

F

V

N

N

T

T

I

V

N
|
N

I

L

S
|
S

T
|
T

T

M

A

A

T

A

F

I

V

V

A

V

A

A

G

G

A

V

N

P

V

V

A

V

K
|
K

H

H

G

G

N

N

R

R

S
x
A

V
x
A

S

S

S
|
S

K

L

S

S

G
|
G

S

G

S

A

D

D

L

T

L

L

G

E

Q

A

F

L

G

G

I

V

E

R

L

I

T

D

M

L

A

G

P

P

Q

D

T

L

A

V

A

A

N

R

C

S

L

L

E

A

A

E

L

V

G

G

I

I

C

G

F

F

L

C

F

F

A

A

P
|
P

H

R

Y

F

H

H

G

P

G

S

V

Y

K
x
R

H

H

A

A

V
x
A

P
x
A

V

V

R

R

Q

R

K

E

L

I

A

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G

G

P

P

L

L

I

T

N

N

P

P

S

A

H

R

P

P

D

R

F

A

M

G

L

L

L

I

G

G

V

C

Y

A

S

F

E

A

T

D

L

L

V

A

R

E

P

V

I

M

A

A

E

G

V

V

L

F

C

A

A

A

L

-

G

-

V

-

K

R

R

R

A

S

-

S

-

V

M

L

V

V

H

H

G

G

-

D

E

D

G

G

L

L

D

D

E
|
E

V

L

A

T

L

T

H

T

G

T

T

T

T

S

Q

T

V

I

C

W

E

R

L

V

R

A

N

A

G

G

E

S

L

V

V

D

D

K

Y

L

S

T

L

F

S

D

P

P

K

A

D

G

F

F

G

G

L

F

E

A

P

R

V

A

Q

Q

V

L

T

D

E

Q

L

L

E

A

G

G

S

D

P

A

E

Q

D

A

N

N

A

A

L

A

I

A

T

V

A

R

A

A

I

V

L

L

K

G

G

G

E

-

G

A

K

R

A

G

A

P

H

V

R

R

D

D

A

A

V

V

A

V

L

L

N

N

A

A

G

A

C

G

A

A

L

I

Y

V

V

A

-

H

A

A

G

G

L

L

A

S

D

E

T

W

P

L

Q

P

A

A

-

W

-

E

G

G

F

L

K

R

L

R

A

A

I

S

D

A

T

A

L

I

A

D

S

T

G

G

K

A

A

A

Y

E

E

Q

K

L

L

L

I

A

G

R

L

W

A

V

A

R

M

F

S

G

Q

R

Q

Q

active site: V82 (= V84)
binding 4-methyl-2-{[2-methyl-6-(methylsulfonyl)phenyl]amino}benzoic acid: P156 (= P158), R163 (≠ K165), A166 (≠ V168), A167 (≠ P169)
binding magnesium ion: S95 (= S97), E228 (= E230)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G86 (= G88), N93 (= N95), S95 (= S97), T96 (= T98), K111 (= K113), A116 (≠ S118), A117 (≠ V119), S119 (= S121), G122 (= G124)

4zofA Lobenzarit-like inhibitor bound in the active site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
35% identity, 97% coverage: 7:341/344 of query aligns to 5:342/342 of 4zofA

query
sites
4zofA

Q

Q

L

I

L

L

D

G

K

R

T

L

Y

T

G

D

G

N

N

R

P

D

L

L

S

A

R

R

E

G

E

Q

A

A

K

A

C

W

L

A

F

M

A

D

S

Q

L

I

V

M

R

T

G

G

E

N

L

A

D

R

E

P

V

A

T

Q

I

I

A

A

L

F

L

A

M

V

A

A

L

M

K

T

V

M

R

K

G

A

E

P

T

T

I

A

D

D

E

E

I

V

S

G

G

E

A

L

A

A

D

G

A

V

M

M

R

L

E

S

A

H

A

A

N

H

S

P

F

L

P

P

R

A

P

D

A

T

L

V

D

P

G

D

I

A

L

V

D

D

I

V

V
|
V

G
|
G

T

T

G
|
G

D

D

G

G

F

V

N

N

T

T

I

V

N
|
N

I

L

S
|
S

T
|
T

T

M

A

A

T

A

F

I

V

V

A

V

A

A

G

G

A

V

N

P

V

V

A

V

K
|
K

H

H

G
|
G

N
|
N

R

R

S
x
A

V
x
A

S

S

S
|
S

K

L

S

S

G
|
G

S

G

S

A

D

D

L

T

L

L

G

E

Q

A

F

L

G

G

I

V

E

R

L

I

T

D

M

L

A

G

P

P

Q

D

T

L

A

V

A

A

N

R

C

S

L

L

E

A

A

E

L

V

G

G

I

I

C

G

F

F

L

C

F

F

A
|
A

P

P

H

R

Y

F

H
|
H

G

P

G

S

V
x
Y

K

R

H

H

A

A

V

A

P

A

V

V

R
|
R

Q

R

K

E

L

I

A

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G
|
G

P

P

L

L

I

T

N

N

P

P

S

A

H

R

P

P

D

R

F

A

M

G

L

L

L

I

G

G

V

C

Y

A

S

F

E

A

T

D

L

L

V

A

R

E

P

V

I

M

A

A

E

G

V

V

L

F

C

A

A

A

L

-

G

-

V

-

K

R

R

R

A

S

-

S

-

V

M

L

V

V

H

H

G

G

-

D

E

D

G

G

L

L

D
|
D

E
|
E

V

L

A

T

L

T

H

T

G

T

T

T

T

S

Q

T

V

I

C

W

E

R

L

V

R

A

N

A

G

G

E

S

L

V

V

D

D

K

Y

L

S

T

L

F

S

D

P

P

K

A

D

G

F

F

G

G

L

F

E

A

P

R

V

A

Q

Q

V

L

T

D

E

Q

L

L

E

A

G

G

S

D

P

A

E

Q

D

A

N

N

A

A

L

A

I

A

T

V

A

R

A

A

I

V

L

L

K

G

G

G

E

-

G

A

K

R

A

G

A

P

H

V

R

R

D

D

A

A

V

V

A

V

L

L

N

N

A

A

G

A

C

G

A

A

L

I

Y

V

V

A

-

H

A

A

G

G

L

L

A

S

D

E

T

W

P

L

Q

P

A

A

-

W

-

E

G

G

F

L

K

R

L

R

A

A

I

S

D

A

T

A

L

I

A

D

S

T

G

G

K

A

A

A

Y

E

E

Q

K

L

L

L

I

A

G

R

L

W

A

V

A

R

M

F

S

G

Q

R

Q

Q

active site: V82 (= V84)
binding 2-[(2-carboxy-5-nitrophenyl)amino]-3-methylbenzoic acid: G113 (= G115), N114 (= N116), A155 (= A157), H159 (= H161), Y162 (≠ V164), R169 (= R171), G182 (= G184)
binding magnesium ion: S95 (= S97), D227 (= D229), E228 (= E230), E228 (= E230)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G85), G85 (= G87), G86 (= G88), N93 (= N95), S95 (= S97), T96 (= T98), K111 (= K113), A116 (≠ S118), A117 (≠ V119), S119 (= S121), G122 (= G124)

P9WFX5 Anthranilate phosphoribosyltransferase; EC 2.4.2.18 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
37% identity, 88% coverage: 7:309/344 of query aligns to 29:331/370 of P9WFX5

query
sites
P9WFX5

Q

Q

L

I

L

L

D

G

K

R

T

L

Y

T

G

D

G

N

N

R

P

D

L

L

S

A

R

R

E

G

E

Q

A

A

K

A

C

W

L

A

F

M

A

D

S

Q

L

I

V

M

R

T

G

G

E

N

L

A

D

R

E

P

V

A

T

Q

I

I

A

A

L

F

L

A

M

V

A

A

L

M

K

T

V

M

R

K

G

A

E

P

T

T

I

A

D

D

E

E

I

V

S

G

G

E

A

L

A

A

D

G

A

V

M

M

R

L

E

S

A

H

A

A

N

H

S

P

F

L

P

P

R

A

P

D

A

T

L

V

D

P

G

D

I

A

L

V

D

D

I

V

V

V

G
|
G

T

T

G

G

D

D

G

G

F

V

N

N

T
|
T

I

V

N

N

I

L

S

S

T

T

T

M

A

A

T

A

F

I

V

V

A

V

A

A

G

G

A

V

N

P

V

V

A

V

K

K

H

H

G

G

N

N

R

R

S

A

V

A

S

S

K

L

S

S

G

G

S
x
G

S

A

D

D

L

T

L

L

G

E

Q

A

F

L

G

G

I

V

E

R

L

I

T

D

M

L

A

G

P

P

Q

D

T

L

A

V

A

A

N

R

C

S

L

L

E

A

A

E

L

V

G

G

I

I

C

G

F

F

L

C

F

F

A

A

P

P

H

R

Y

F

H

H

G

P

G

S

V

Y

K

R

H

H

A

A

V

A

P

A

V

V

R

R

Q

R

K

E

L

I

A

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G

G

P

P

L

L

I

T

N

N

P

P

S

A

H

R

P

P

D

R

F

A

M

G

L

L

L

I

G

G

V

C

Y

A

S

F

E

A

T

D

L

L

V

A

R

E

P

V

I

M

A

A

E

G

V

V

L

F

C

A

A

A

L

-

G

-

V

-

K

R

R

R

A

S

-

S

-

V

M

L

V

V

H

H

G

G

-

D

E

D

G

G

L

L

D

D

E

E

V

L

A

T

L

T

H

T

G

T

T

T

T

S

Q

T

V

I

C

W

E

R

L

V

R

A

N

A

G

G

E

S

L

V

V

D

D

K

Y

L

S

T

L

F

S

D

P

P

K

A

D

G

F

F

G

G

L

F

E

A

P

R

V

A

Q

Q

V

L

T

D

E

Q

L

L

E

A

G

G

S

D

P

A

E

Q

D

A

N

N

A

A

L

A

I

A

T

V

A

R

A

A

I

V

L

L

K

G

G

G

E

-

G

A

K

R

A

G

A

P

H

V

R

R

D

D

A

A

V

V

A

V

L

L

N

N

A

A

G

A

C

G

A

A

L

I

Y

V

V

A

-

H

A

A

G

G

L

L

A

S

G107 (= G85) binding
T115 (= T93) binding
G147 (≠ S125) binding

5c7sA Prpp complexed with two mn2+ in the active site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
35% identity, 97% coverage: 7:341/344 of query aligns to 6:344/344 of 5c7sA

query
sites
5c7sA

Q

Q

L

I

L

L

D

G

K

R

T

L

Y

T

G

D

G

N

N

R

P

D

L

L

S

A

R

R

E

G

E

Q

A

A

K

A

C

W

L

A

F

M

A

D

S

Q

L

I

V

M

R

T

G

G

E

N

L

A

D

R

E

P

V

A

T

Q

I

I

A

A

L

F

L

A

M

V

A

A

L

M

K

T

V

M

R

K

G

A

E

P

T

T

I

A

D

D

E

E

I

V

S

G

G

E

A

L

A

A

D

G

A

V

M

M

R

L

E

S

A

H

A

A

N

H

S

P

F

L

P

P

R

A

P

D

A

T

L

V

D

P

G

D

I

A

L

V

D

D

I

V

V
|
V

G
|
G

T

T

G
|
G

D

D

G

G

F

V

N

N

T

T

I

V

N
|
N

I

L

S
|
S

T
|
T

T

M

A

A

T

A

F

I

V

V

A

V

A

A

G

G

A

V

N

P

V

V

A

V

K
|
K

H

H

G

G

N
|
N

R

R

S

A

V

A

S

S

S
|
S

K

L

S

S

G

G

S

G

S

A

D

D

L

T

L

L

G

E

Q

A

F

L

G

G

I

V

E

R

L

I

T

D

M

L

A

G

P

P

Q

D

T

L

A

V

A

A

N

R

C

S

L

L

E

A

A

E

L

V

G

G

I

I

C

G

F

F

L

C

F

F

A

A

P

P

H

R

Y

F

H

H

G

P

G

S

V

Y

K

R

H

H

A

A

V

A

P

A

V

V

R

R

Q

R

K

E

L

I

A

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G

G

P

P

L

L

I

T

N

N

P

P

S

A

H

R

P

P

D

R

F

A

M

G

L

L

L

I

G

G

V

C

Y

A

S

F

E

A

T

D

L

L

V

A

R

E

P

V

I

M

A

A

E

G

V

V

L

F

C

A

A

A

L

-

G

-

V

-

K

R

R

R

A

S

-

S

-

V

M

L

V

V

H

H

G

G

-

D

E

D

G

G

L

L

D
|
D

E
|
E

V

L

A

T

L

T

H

T

G

T

T

T

T

S

Q

T

V

I

C

W

E

R

L

V

R

A

N

A

G

G

E

S

L

V

V

D

D

K

Y

L

S

T

L

F

S

D

P

P

K

A

D

G

F

F

G

G

L

F

E

A

P

R

V

A

Q

Q

V

L

T

D

E

Q

L

L

E

A

G

G

S

D

P

A

E

Q

D

A

N

N

A

A

L

A

I

A

T

V

A

R

A

A

I

V

L

L

K

G

G

G

E

-

G

A

K

R

A

G

A

P

H

V

R

R

D

D

A

A

V

V

A

V

L

L

N

N

A

A

G

A

C

G

A

A

L

I

Y

V

V

A

-

H

A

A

G

G

L

L

A

S

D

S

-

E

-

W

-

L

-

P

T

A

P

W

Q

E

A

E

G

G

F

L

K

R

L

R

A

A

I

S

D

A

T

A

L

I

A

D

S

T

G

G

K

A

A

A

Y

E

E

Q

K

L

L

L

I

A

G

R

L

W

A

V

A

R

M

F

S

G

Q

R

Q

Q

active site: V83 (= V84)
binding manganese (ii) ion: G84 (= G85), S96 (= S97), D228 (= D229), E229 (= E230), E229 (= E230)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V83 (= V84), G84 (= G85), G86 (= G87), G87 (= G88), N94 (= N95), S96 (= S97), T97 (= T98), K112 (= K113), N115 (= N116), S120 (= S121)

5c2lA Magnesium soaked into the active site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
37% identity, 88% coverage: 7:309/344 of query aligns to 7:309/349 of 5c2lA

query
sites
5c2lA

Q

Q

L

I

L

L

D

G

K

R

T

L

Y

T

G

D

G

N

N

R

P

D

L

L

S

A

R

R

E

G

E

Q

A

A

K

A

C

W

L

A

F

M

A

D

S

Q

L

I

V

M

R

T

G

G

E

N

L

A

D

R

E

P

V

A

T

Q

I

I

A

A

L

F

L

A

M

V

A

A

L

M

K

T

V

M

R

K

G

A

E

P

T

T

I

A

D

D

E

E

I

V

S

G

G

E

A

L

A

A

D

G

A

V

M

M

R

L

E

S

A

H

A

A

N

H

S

P

F

L

P

P

R

A

P

D

A

T

L

V

D

P

G

D

I

A

L

V

D

D

I

V

V
|
V

G

G

T

T

G

G

D

D

G

G

F

V

N

N

T

T

I

V

N

N

I

L

S

S

T

T

T

M

A

A

T

A

F

I

V

V

A

V

A

A

G

G

A

V

N

P

V

V

A

V

K

K

H

H

G

G

N

N

R

R

S

A

V

A

S

S

K

L

S

S

G

G

S

G

S

A

D

D

L

T

L

L

G

E

Q

A

F

L

G

G

I

V

E

R

L

I

T

D

M

L

A

G

P

P

Q

D

T

L

A

V

A

A

N

R

C

S

L

L

E

A

A

E

L

V

G

G

I

I

C

G

F

F

L

C

F

F

A

A

P

P

H

R

Y

F

H

H

G

P

G

S

V

Y

K

R

H

H

A

A

V

A

P

A

V

V

R

R

Q

R

K

E

L

I

A

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G

G

P

P

L

L

I

T

N

N

P

P

S

A

H

R

P

P

D

R

F

A

M

G

L

L

L

I

G

G

V

C

Y

A

S

F

E

A

T

D

L

L

V

A

R

E

P

V

I

M

A

A

E

G

V

V

L

F

C

A

A

A

L

-

G

-

V

-

K

R

R

R

A

S

-

S

-

V

M

L

V

V

H

H

G

G

-

D

E

D

G

G

L

L

D
|
D

E
|
E

V

L

A

T

L

T

H

T

G

T

T

T

T

S

Q

T

V

I

C

W

E

R

L

V

R

A

N

A

G

G

E

S

L

V

V

D

D

K

Y

L

S

T

L

F

S

D

P

P

K

A

D

G

F

F

G

G

L

F

E

A

P

R

V

A

Q

Q

V

L

T

D

E

Q

L

L

E

A

G

G

S

D

P

A

E

Q

D

A

N

N

A

A

L

A

I

A

T

V

A

R

A

A

I

V

L

L

K

G

G

G

E

-

G

A

K

R

A

G

A

P

H

V

R

R

D

D

A

A

V

V

A

V

L

L

N

N

A

A

G

A

C

G

A

A

L

I

Y

V

V

A

-

H

A

A

G

G

L

L

A

S

active site: V84 (= V84)
binding magnesium ion: D229 (= D229), E230 (= E230)

4n93A Alternative substrates of mycobacterium tuberculosis anthranilate phosphoribosyl transferase (see paper)
37% identity, 88% coverage: 7:309/344 of query aligns to 6:308/346 of 4n93A

query
sites
4n93A

Q

Q

L

I

L

L

D

G

K

R

T

L

Y

T

G

D

G

N

N

R

P

D

L

L

S

A

R

R

E

G

E

Q

A

A

K

A

C

W

L

A

F

M

A

D

S

Q

L

I

V

M

R

T

G

G

E

N

L

A

D

R

E

P

V

A

T

Q

I

I

A

A

L

F

L

A

M

V

A

A

L

M

K

T

V

M

R

K

G

A

E

P

T

T

I

A

D

D

E

E

I

V

S

G

G

E

A

L

A

A

D

G

A

V

M

M

R

L

E

S

A

H

A

A

N

H

S

P

F

L

P

P

R

A

P

D

A

T

L

V

D

P

G

D

I

A

L

V

D

D

I

V

V
|
V

G
|
G

T
|
T

G
|
G

D

D

G

G

F

V

N

N

T

T

I

V

N
|
N

I

L

S
|
S

T
|
T

T

M

A

A

T

A

F

I

V

V

A

V

A

A

G

G

A

V

N

P

V

V

A

V

K
|
K

H
|
H

G

G

N
|
N

R

R

S
x
A

V
x
A

S

S

S
|
S

K

L

S

S

G
|
G

S

G

S

A

D

D

L

T

L

L

G

E

Q

A

F

L

G

G

I

V

E

R

L

I

T

D

M

L

A

G

P

P

Q

D

T

L

A

V

A

A

N

R

C

S

L

L

E

A

A

E

L

V

G

G

I

I

C

G

F

F

L

C

F

F

A
|
A

P
|
P

H

R

Y

F

H
|
H

G

P

G

S

V
x
Y

K

R

H

H

A

A

V
x
A

P

A

V

V

R
|
R

Q

R

K

E

L

I

A

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G
|
G

P
|
P

L

L

I

T

N

N

P

P

S

A

H

R

P

P

D

R

F

A

M

G

L

L

L

I

G

G

V

C

Y

A

S

F

E

A

T

D

L

L

V

A

R

E

P

V

I

M

A

A

E

G

V

V

L

F

C

A

A

A

L

-

G

-

V

-

K

R

R

R

A

S

-

S

-

V

M

L

V

V

H

H

G

G

-

D

E

D

G

G

L

L

D
|
D

E
|
E

V

L

A

T

L

T

H

T

G

T

T

T

T

S

Q

T

V

I

C

W

E

R

L

V

R

A

N

A

G

G

E

S

L

V

V

D

D

K

Y

L

S

T

L

F

S

D

P

P

K

A

D

G

F

F

G

G

L

F

E

A

P

R

V

A

Q

Q

V

L

T

D

E

Q

L

L

E

A

G

G

S

D

P

A

E

Q

D

A

N

N

A

A

L

A

I

A

T

V

A

R

A

A

I

V

L

L

K

G

G

G

E

-

G

A

K

R

A

G

A

P

H

V

R

R

D

D

A

A

V

V

A

V

L

L

N

N

A

A

G

A

C

G

A

A

L

I

Y

V

V

A

-

H

A

A

G

G

L

L

A

S

active site: V83 (= V84)
binding 2-amino-6-methylbenzoic acid: V83 (= V84), G84 (= G85), T85 (= T86), H113 (= H114), N115 (= N116), N115 (= N116), A156 (= A157), P157 (= P158), H160 (= H161), Y163 (≠ V164), A167 (≠ V168), R170 (= R171), G183 (= G184), P184 (= P185)
binding magnesium ion: S96 (= S97), D228 (= D229), E229 (= E230), E229 (= E230)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G84 (= G85), G86 (= G87), G87 (= G88), N94 (= N95), S96 (= S97), T97 (= T98), K112 (= K113), A117 (≠ S118), A118 (≠ V119), S120 (= S121), G123 (= G124)

4giuA Bianthranilate-like analogue bound in inner site of anthranilate phosphoribosyltransferase (anprt; trpd). (see paper)
37% identity, 88% coverage: 7:309/344 of query aligns to 6:308/346 of 4giuA

query
sites
4giuA

Q

Q

L

I

L

L

D

G

K

R

T

L

Y

T

G

D

G

N

N

R

P

D

L

L

S

A

R

R

E

G

E

Q

A

A

K

A

C

W

L

A

F

M

A

D

S

Q

L

I

V

M

R

T

G

G

E

N

L

A

D

R

E

P

V

A

T

Q

I

I

A

A

L

F

L

A

M

V

A

A

L

M

K

T

V

M

R

K

G

A

E

P

T

T

I

A

D

D

E

E

I

V

S

G

G

E

A

L

A

A

D

G

A

V

M

M

R

L

E

S

A

H

A

A

N

H

S

P

F

L

P

P

R

A

P

D

A

T

L

V

D

P

G

D

I

A

L

V

D

D

I

V

V
|
V

G
|
G

T

T

G
|
G

D

D

G

G

F

V

N

N

T

T

I

V

N
|
N

I

L

S
|
S

T
|
T

T

M

A

A

T

A

F

I

V

V

A

V

A

A

G

G

A

V

N

P

V

V

A

V

K
|
K

H

H

G
|
G

N
|
N

R

R

S
x
A

V
x
A

S
|
S

K

L

S

S

G
|
G

S

G

S

A

D

D

L

T

L

L

G

E

Q

A

F

L

G

G

I

V

E

R

L

I

T

D

M

L

A

G

P

P

Q

D

T

L

A

V

A

A

N

R

C

S

L

L

E

A

A

E

L

V

G

G

I

I

C

G

F

F

L

C

F

F

A
|
A

P

P

H

R

Y

F

H
|
H

G

P

G

S

V
x
Y

K

R

H

H

A

A

V

A

P

A

V

V

R
|
R

Q

R

K

E

L

I

A

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G
|
G

P

P

L

L

I

T

N

N

P

P

S

A

H

R

P

P

D

R

F

A

M

G

L

L

L

I

G

G

V

C

Y

A

S

F

E

A

T

D

L

L

V

A

R

E

P

V

I

M

A

A

E

G

V

V

L

F

C

A

A

A

L

-

G

-

V

-

K

R

R

R

A

S

-

S

-

V

M

L

V

V

H

H

G

G

-

D

E

D

G

G

L

L

D
|
D

E
|
E

V

L

A

T

L

T

H

T

G

T

T

T

T

S

Q

T

V

I

C

W

E

R

L

V

R

A

N

A

G

G

E

S

L

V

V

D

D

K

Y

L

S

T

L

F

S

D

P

P

K

A

D

G

F

F

G

G

L

F

E

A

P

R

V

A

Q

Q

V

L

T

D

E

Q

L

L

E

A

G

G

S

D

P

A

E

Q

D

A

N

N

A

A

L

A

I

A

T

V

A

R

A

A

I

V

L

L

K

G

G

G

E

-

G

A

K

R

A

G

A

P

H

V

R

R

D

D

A

A

V

V

A

V

L

L

N

N

A

A

G

A

C

G

A

A

L

I

Y

V

V

A

-

H

A

A

G

G

L

L

A

S

active site: V83 (= V84)
binding 2-[(2-carboxy-5-methylphenyl)amino]-3-methylbenzoic acid: G114 (= G115), N115 (= N116), A156 (= A157), H160 (= H161), Y163 (≠ V164), R170 (= R171), G183 (= G184)
binding magnesium ion: S96 (= S97), D228 (= D229), E229 (= E230), E229 (= E230)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G84 (= G85), G86 (= G87), G87 (= G88), N94 (= N95), S96 (= S97), T97 (= T98), K112 (= K113), N115 (= N116), A117 (≠ S118), A118 (≠ V119), S119 (= S120), S120 (= S121), G123 (= G124)

4owoA Anthranilate phosphoribosyl transferase from mycobacterium tuberculosis in complex with 6-fluoroanthranilate, prpp and magnesium (see paper)
37% identity, 88% coverage: 7:309/344 of query aligns to 7:309/348 of 4owoA

query
sites
4owoA

Q

Q

L

I

L

L

D

G

K

R

T

L

Y

T

G

D

G

N

N

R

P

D

L

L

S

A

R

R

E

G

E

Q

A

A

K

A

C

W

L

A

F

M

A

D

S

Q

L

I

V

M

R

T

G

G

E

N

L

A

D

R

E

P

V

A

T

Q

I

I

A

A

L

F

L

A

M

V

A

A

L

M

K

T

V

M

R

K

G

A

E

P

T

T

I

A

D

D

E

E

I

V

S

G

G

E

A

L

A

A

D

G

A

V

M

M

R

L

E

S

A

H

A

A

N

H

S

P

F

L

P

P

R

A

P

D

A

T

L

V

D

P

G

D

I

A

L

V

D

D

I

V

V
|
V

G
|
G

T
|
T

G
|
G

D

D

G

G

F

V

N

N

T

T

I

V

N
|
N

I

L

S
|
S

T
|
T

T

M

A

A

T

A

F

I

V

V

A

V

A

A

G

G

A

V

N

P

V

V

A

V

K
|
K

H
|
H

G

G

N
|
N

R

R

S
x
A

V
x
A

S
|
S

K

L

S

S

G
|
G

S

G

S

A

D

D

L

T

L

L

G

E

Q

A

F

L

G

G

I

V

E

R

L

I

T

D

M

L

A

G

P

P

Q

D

T

L

A

V

A

A

N

R

C

S

L

L

E

A

A

E

L

V

G

G

I

I

C

G

F

F

L

C

F

F

A
|
A

P
|
P

H

R

Y

F

H
|
H

G

P

G

S

V
x
Y

K

R

H

H

A

A

V
x
A

P

A

V

V

R
|
R

Q

R

K

E

L

I

A

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G
|
G

P

P

L

L

I

T

N

N

P

P

S

A

H

R

P

P

D

R

F

A

M

G

L

L

L

I

G

G

V

C

Y

A

S

F

E

A

T

D

L

L

V

A

R

E

P

V

I

M

A

A

E

G

V

V

L

F

C

A

A

A

L

-

G

-

V

-

K

R

R

R

A

S

-

S

-

V

M

L

V

V

H

H

G

G

-

D

E

D

G

G

L

L

D
|
D

E
|
E

V

L

A

T

L

T

H

T

G

T

T

T

T

S

Q

T

V

I

C

W

E

R

L

V

R

A

N

A

G

G

E

S

L

V

V

D

D

K

Y

L

S

T

L

F

S

D

P

P

K

A

D

G

F

F

G

G

L

F

E

A

P

R

V

A

Q

Q

V

L

T

D

E

Q

L

L

E

A

G

G

S

D

P

A

E

Q

D

A

N

N

A

A

L

A

I

A

T

V

A

R

A

A

I

V

L

L

K

G

G

G

E

-

G

A

K

R

A

G

A

P

H

V

R

R

D

D

A

A

V

V

A

V

L

L

N

N

A

A

G

A

C

G

A

A

L

I

Y

V

V

A

-

H

A

A

G

G

L

L

A

S

active site: V84 (= V84)
binding 2-azanyl-6-fluoranyl-benzoic acid: V84 (= V84), G85 (= G85), T86 (= T86), H114 (= H114), N116 (= N116), N116 (= N116), A157 (= A157), P158 (= P158), H161 (= H161), Y164 (≠ V164), A168 (≠ V168), R171 (= R171), G184 (= G184)
binding magnesium ion: S97 (= S97), D229 (= D229), E230 (= E230), E230 (= E230)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V84 (= V84), G85 (= G85), G87 (= G87), G88 (= G88), N95 (= N95), S97 (= S97), T98 (= T98), K113 (= K113), A118 (≠ S118), A119 (≠ V119), S120 (= S120), S121 (= S121), G124 (= G124)

4n5vA Alternative substrates of mycobacterium tuberculosis anthranilate phosphoribosyl transferase (see paper)
37% identity, 88% coverage: 7:309/344 of query aligns to 7:309/347 of 4n5vA

query
sites
4n5vA

Q

Q

L

I

L

L

D

G

K

R

T

L

Y

T

G

D

G

N

N

R

P

D

L

L

S

A

R

R

E

G

E

Q

A

A

K

A

C

W

L

A

F

M

A

D

S

Q

L

I

V

M

R

T

G

G

E

N

L

A

D

R

E

P

V

A

T

Q

I

I

A

A

L

F

L

A

M

V

A

A

L

M

K

T

V

M

R

K

G

A

E

P

T

T

I

A

D

D

E

E

I

V

S

G

G

E

A

L

A

A

D

G

A

V

M

M

R

L

E

S

A

H

A

A

N

H

S

P

F

L

P

P

R

A

P

D

A

T

L

V

D

P

G

D

I

A

L

V

D

D

I

V

V
|
V

G
|
G

T

T

G
|
G

D

D

G

G

F

V

N

N

T

T

I

V

N
|
N

I

L

S
|
S

T
|
T

T

M

A

A

T

A

F

I

V

V

A

V

A

A

G

G

A

V

N

P

V

V

A

V

K
|
K

H
|
H

G
|
G

N
|
N

R

R

S

A

V
x
A

S
|
S

K

L

S

S

G
|
G

S

G

S

A

D

D

L

T

L

L

G

E

Q

A

F

L

G

G

I

V

E

R

L

I

T

D

M

L

A

G

P

P

Q

D

T

L

A

V

A

A

N

R

C

S

L

L

E

A

A

E

L

V

G

G

I

I

C

G

F

F

L

C

F

F

A
|
A

P
|
P

H

R

Y

F

H
|
H

G

P

G

S

V
x
Y

K
x
R

H

H

A

A

V
x
A

P

A

V

V

R
|
R

Q

R

K

E

L

I

A

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G
|
G

P

P

L

L

I

T

N

N

P

P

S

A

H

R

P

P

D

R

F

A

M

G

L

L

L

I

G

G

V

C

Y

A

S

F

E

A

T

D

L

L

V

A

R

E

P

V

I

M

A

A

E

G

V

V

L

F

C

A

A

A

L

-

G

-

V

-

K

R

R

R

A

S

-

S

-

V

M

L

V

V

H

H

G

G

-

D

E

D

G

G

L

L

D
|
D

E
|
E

V

L

A

T

L

T

H

T

G

T

T

T

T

S

Q

T

V

I

C

W

E

R

L

V

R

A

N

A

G

G

E

S

L

V

V

D

D

K

Y

L

S

T

L

F

S

D

P

P

K

A

D

G

F

F

G

G

L

F

E

A

P

R

V

A

Q

Q

V

L

T

D

E

Q

L

L

E

A

G

G

S

D

P

A

E

Q

D

A

N

N

A

A

L

A

I

A

T

V

A

R

A

A

I

V

L

L

K

G

G

G

E

-

G

A

K

R

A

G

A

P

H

V

R

R

D

D

A

A

V

V

A

V

L

L

N

N

A

A

G

A

C

G

A

A

L

I

Y

V

V

A

-

H

A

A

G

G

L

L

A

S

active site: V84 (= V84)
binding 2-amino-4-fluorobenzoic acid: V84 (= V84), G85 (= G85), H114 (= H114), G115 (= G115), N116 (= N116), A157 (= A157), A157 (= A157), P158 (= P158), H161 (= H161), Y164 (≠ V164), R165 (≠ K165), A168 (≠ V168), R171 (= R171), G184 (= G184)
binding magnesium ion: S97 (= S97), D229 (= D229), E230 (= E230), E230 (= E230)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G85 (= G85), G87 (= G87), G88 (= G88), N95 (= N95), S97 (= S97), T98 (= T98), K113 (= K113), A119 (≠ V119), S120 (= S120), S121 (= S121), G124 (= G124)

4owmA Anthranilate phosphoribosyl transferase from mycobacterium tuberculosis in complex with 3-fluoroanthranilate, prpp and magnesium (see paper)
37% identity, 88% coverage: 7:309/344 of query aligns to 5:307/346 of 4owmA

query
sites
4owmA

Q

Q

L

I

L

L

D

G

K

R

T

L

Y

T

G

D

G

N

N

R

P

D

L

L

S

A

R

R

E

G

E

Q

A

A

K

A

C

W

L

A

F

M

A

D

S

Q

L

I

V

M

R

T

G

G

E

N

L

A

D

R

E

P

V

A

T

Q

I

I

A

A

L

F

L

A

M

V

A

A

L

M

K

T

V

M

R

K

G

A

E

P

T

T

I

A

D

D

E

E

I

V

S

G

G

E

A

L

A

A

D

G

A

V

M

M

R

L

E

S

A

H

A

A

N

H

S

P

F

L

P

P

R

A

P

D

A

T

L

V

D

P

G

D

I

A

L

V

D

D

I

V

V
|
V

G
|
G

T

T

G

G

G
|
G

D

D

G

G

F

V

N

N

T

T

I

V

N
|
N

I

L

S
|
S

T
|
T

T

M

A

A

T

A

F

I

V

V

A

V

A

A

G

G

A

V

N

P

V

V

A

V

K
|
K

H

H

G

G

N

N

R

R

S

A

V
x
A

S
|
S

K

L

S

S

G
|
G

S

G

S

A

D

D

L

T

L

L

G

E

Q

A

F

L

G

G

I

V

E

R

L

I

T

D

M

L

A

G

P

P

Q

D

T

L

A

V

A

A

N

R

C

S

L

L

E

A

A

E

L

V

G

G

I

I

C

G

F

F

L

C

F

F

A

A

P
|
P

H

R

Y

F

H
|
H

G

P

G

S

V
x
Y

K
x
R

H

H

A

A

V
x
A

P

A

V

V

R

R

Q
x
R

K

E

L

I

A

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G

G

P

P

L

L

I

T

N

N

P

P

S

A

H

R

P

P

D

R

F

A

M

G

L

L

L

I

G

G

V

C

Y

A

S

F

E

A

T

D

L

L

V

A

R

E

P

V

I

M

A

A

E

G

V

V

L

F

C

A

A

A

L

-

G

-

V

-

K

R

R

R

A

S

-

S

-

V

M

L

V

V

H

H

G

G

-

D

E

D

G

G

L

L

D
|
D

E
|
E

V

L

A

T

L

T

H

T

G

T

T

T

T

S

Q

T

V

I

C

W

E

R

L

V

R

A

N

A

G

G

E

S

L

V

V

D

D

K

Y

L

S

T

L

F

S

D

P

P

K

A

D

G

F

F

G

G

L

F

E

A

P

R

V

A

Q

Q

V

L

T

D

E

Q

L

L

E

A

G

G

S

D

P

A

E

Q

D

A

N

N

A

A

L

A

I

A

T

V

A

R

A

A

I

V

L

L

K

G

G

G

E

-

G

A

K

R

A

G

A

P

H

V

R

R

D

D

A

A

V

V

A

V

L

L

N

N

A

A

G

A

C

G

A

A

L

I

Y

V

V

A

-

H

A

A

G

G

L

L

A

S

active site: V82 (= V84)
binding 2-azanyl-3-fluoranyl-benzoic acid: P156 (= P158), H159 (= H161), Y162 (≠ V164), R163 (≠ K165), A166 (≠ V168), R170 (≠ Q172)
binding magnesium ion: S95 (= S97), D227 (= D229), E228 (= E230), E228 (= E230)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G85), G86 (= G88), N93 (= N95), S95 (= S97), T96 (= T98), K111 (= K113), A117 (≠ V119), S118 (= S120), S119 (= S121), G122 (= G124)

4gkmA Bianthranilate-like analogue bound in the outer site of anthranilate phosphoribosyltransferase (anprt; trpd) (see paper)
37% identity, 88% coverage: 7:309/344 of query aligns to 5:307/346 of 4gkmA

query
sites
4gkmA

Q

Q

L

I

L

L

D

G

K

R

T

L

Y

T

G

D

G

N

N

R

P

D

L

L

S

A

R

R

E

G

E

Q

A

A

K

A

C

W

L

A

F

M

A

D

S

Q

L

I

V

M

R

T

G

G

E

N

L

A

D

R

E

P

V

A

T

Q

I

I

A

A

L

F

L

A

M

V

A

A

L

M

K

T

V

M

R

K

G

A

E

P

T

T

I

A

D

D

E

E

I

V

S

G

G

E

A

L

A

A

D

G

A

V

M

M

R

L

E

S

A

H

A

A

N

H

S

P

F

L

P

P

R

A

P

D

A

T

L

V

D

P

G

D

I

A

L

V

D

D

I

V

V
|
V

G

G

T

T

G

G

G
|
G

D

D

G

G

F

V

N

N

T

T

I

V

N
|
N

I

L

S
|
S

T
|
T

T

M

A

A

T

A

F

I

V

V

A

V

A

A

G

G

A

V

N

P

V

V

A

V

K
|
K

H

H

G

G

N
|
N

R

R

S

A

V
x
A

S
|
S

K

L

S

S

G
|
G

S

G

S

A

D

D

L

T

L

L

G

E

Q

A

F

L

G

G

I

V

E

R

L

I

T

D

M

L

A

G

P

P

Q

D

T

L

A

V

A

A

N

R

C

S

L

L

E

A

A

E

L

V

G

G

I

I

C

G

F

F

L

C

F

F

A
|
A

P
|
P

H

R

Y

F

H

H

G

P

G

S

V
x
Y

K
x
R

H

H

A

A

V
x
A

P

A

V

V

R
|
R

Q
x
R

K

E

L

I

A

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G

G

P

P

L

L

I

T

N

N

P

P

S

A

H

R

P

P

D

R

F

A

M

G

L

L

L

I

G

G

V

C

Y

A

S

F

E

A

T

D

L

L

V

A

R

E

P

V

I

M

A

A

E

G

V

V

L

F

C

A

A

A

L

-

G

-

V

-

K

R

R

R

A

S

-

S

-

V

M

L

V

V

H

H

G

G

-

D

E

D

G

G

L

L

D
|
D

E
|
E

V

L

A

T

L

T

H

T

G

T

T

T

T

S

Q

T

V

I

C

W

E

R

L

V

R

A

N

A

G

G

E

S

L

V

V

D

D

K

Y

L

S

T

L

F

S

D

P

P

K

A

D

G

F

F

G

G

L

F

E

A

P

R

V

A

Q

Q

V

L

T

D

E

Q

L

L

E

A

G

G

S

D

P

A

E

Q

D

A

N

N

A

A

L

A

I

A

T

V

A

R

A

A

I

V

L

L

K

G

G

G

E

-

G

A

K

R

A

G

A

P

H

V

R

R

D

D

A

A

V

V

A

V

L

L

N

N

A

A

G

A

C

G

A

A

L

I

Y

V

V

A

-

H

A

A

G

G

L

L

A

S

active site: V82 (= V84)
binding 2-[(2-carboxyphenyl)amino]-5-methylbenzoic acid: N114 (= N116), A155 (= A157), P156 (= P158), Y162 (≠ V164), R163 (≠ K165), A166 (≠ V168), R169 (= R171), R170 (≠ Q172)
binding magnesium ion: S95 (= S97), D227 (= D229), E228 (= E230), E228 (= E230)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G86 (= G88), N93 (= N95), S95 (= S97), T96 (= T98), K111 (= K113), A117 (≠ V119), S118 (= S120), S119 (= S121), G122 (= G124)

3r6cA Anthranilate phosphoribosyltransferase (trpd) from mycobacterium tuberculosis (complex with inhibitor acs179) (see paper)
37% identity, 88% coverage: 7:309/344 of query aligns to 5:307/346 of 3r6cA

query
sites
3r6cA

Q

Q

L

I

L

L

D

G

K

R

T

L

Y

T

G

D

G

N

N

R

P

D

L

L

S

A

R

R

E

G

E

Q

A

A

K

A

C

W

L

A

F

M

A

D

S

Q

L

I

V

M

R

T

G

G

E

N

L

A

D

R

E

P

V

A

T

Q

I

I

A

A

L

F

L

A

M

V

A

A

L

M

K

T

V

M

R

K

G

A

E

P

T

T

I

A

D

D

E

E

I

V

S

G

G

E

A

L

A

A

D

G

A

V

M
|
M

R

L

E

S

A

H

A

A

N

H

S

P

F

L

P

P

R

A

P

D

A

T

L

V

D

P

G

D

I

A

L

V

D

D

I

V

V
|
V

G
|
G

T

T

G
|
G

D

D

G

G

F

V

N

N

T

T

I

V

N
|
N

I

L

S
|
S

T
|
T

T

M

A

A

T

A

F

I

V

V

A

V

A

A

G

G

A

V

N

P

V

V

A

V

K
|
K

H

H

G

G

N
|
N

R
|
R

S
x
A

V
x
A

S
|
S

K

L

S

S

G
|
G

S
x
G

S

A

D

D

L

T

L

L

G

E

Q

A

F

L

G

G

I

V

E

R

L

I

T

D

M

L

A

G

P

P

Q

D

T

L

A

V

A

A

N

R

C

S

L

L

E

A

A

E

L

V

G

G

I

I

C

G

F

F

L

C

F

F

A
|
A

P
|
P

H

R

Y

F

H
|
H

G

P

G

S

V
x
Y

K

R

H

H

A

A

V
x
A

P

A

V

V

R
|
R

Q

R

K

E

L

I

A

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G
|
G

P

P

L

L

I
x
T

N

N

P

P

S

A

H

R

P

P

D

R

F

A

M

G

L

L

L

I

G

G

V

C

Y

A

S

F

E

A

T

D

L

L

V

A

R

E

P

V

I

M

A

A

E

G

V

V

L

F

C

A

A

A

L

-

G

-

V

-

K

R

R

R

A

S

-

S

-

V

M

L

V

V

H

H

G

G

-

D

E

D

G

G

L

L

D
|
D

E
|
E

V

L

A

T

L

T

H

T

G

T

T

T

T

S

Q

T

V

I

C

W

E
x
R

L

V

R
x
A

N

A

G

G

E

S

L

V

V
x
D

D

K

Y

L

S

T

L

F

S

D

P

P

K

A

D

G

F

F

G

G

L

F

E

A

P

R

V

A

Q

Q

V

L

T

D

E

Q

L

L

E

A

G

G

S

D

P

A

E

Q

D

A

N

N

A

A

L

A

I

A

T

V

A

R

A

A

I

V

L

L

K

G

G

G

E

-

G

A

K

R

A

G

A

P

H

V

R

R

D

D

A

A

V

V

A

V

L

L

N

N

A

A

G

A

C

G

A

A

L

I

Y
x
V

V

A

-

H

A

A

G

G

L

L

A

S

active site: V82 (= V84)
binding 8-methoxyphenanthro[3,4-d][1,3]dioxole-5,6-dicarboxylic acid: M62 (= M64), N114 (= N116), A155 (= A157), P156 (= P158), H159 (= H161), Y162 (≠ V164), A166 (≠ V168), R169 (= R171), G182 (= G184), T185 (≠ I187), R239 (≠ E241), A241 (≠ R243), D246 (≠ V248), V301 (≠ Y304)
binding magnesium ion: S95 (= S97), D227 (= D229), E228 (= E230), E228 (= E230)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G85), G85 (= G87), G86 (= G88), N93 (= N95), S95 (= S97), T96 (= T98), K111 (= K113), N114 (= N116), R115 (= R117), A116 (≠ S118), A117 (≠ V119), S118 (= S120), S119 (= S121), G122 (= G124), G123 (≠ S125)

Sites not aligning to the query:

binding 8-methoxyphenanthro[3,4-d][1,3]dioxole-5,6-dicarboxylic acid: 310, 312

Query Sequence

>6937998 FitnessBrowser__SB2B:6937998
MSKDVRQLLDKTYGGNPLSREEAKCLFASLVRGELDEVTIAALLMALKVRGETIDEISGA
ADAMREAANSFPRPALDGGILDIVGTGGDGFNTINISTTATFVAAAAGANVAKHGNRSVS
SKSGSSDLLGQFGIELTMAPQTAANCLEALGICFLFAPHYHGGVKHAVPVRQKLATRTLF
NVLGPLINPSHPDFMLLGVYSETLVRPIAEVLCALGVKRAMVVHGEGLDEVALHGTTQVC
ELRNGELVDYSLSPKDFGLEPVQVTELEGGSPEDNALITAAILKGEGKAAHRDAVALNAG
CALYVAGLADTPQAGFKLAIDTLASGKAYEKLIGLAAMSQQDKA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory