SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 6938119 FitnessBrowser__SB2B:6938119 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4ho9A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with udp-galactose and utp
68% identity, 93% coverage: 16:299/305 of query aligns to 2:285/294 of 4ho9A

query
sites
4ho9A

K

K

G

G

I

I

L
|
L

A

S

G
|
G

T

S

G

G

S

T

R

R

L

L

Y

Y

P

P

I

L

T

T

R

K

G

V

V

V

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

V

V

Y

Y

P

P

L

L

S

S

V

V

L

L

M

M

L
|
L

A

A

G

G

I

I

R

K

D

D

I

I

L

L

L

I

I

I

S

S

T

T

A

P

S

E

D

D

L

T

A

P

G

R

F

F

Q

E

S

Q

L

L

G

G

D

G

G

G

S

S

G

E

L

L

G

G

I

I

R

S

I

L

S

S

Y

Y

A

A

V

V

Q
|
Q

S

S

K

S

P
|
P

E

D

G
|
G

I
x
L

A

A

Q

Q

A

A

F

F

L

I

I

I

G

G

E

E

D

E

F

F

I

I

G

G

N

D

D

D

K

N

V

V

A

A

L

L

I

V

L
|
L

G

G

D
|
D

N

N

I

I

F

F

Y

Y

G
|
G

Q
x
H

S
x
G

F

F

S
x
T

R

E

Q

L

L

L

Q

Q

Q

R

A

A

D

N

C

R

S

K

L

S

G

G

A

A

T

T

V

I

F

F

A

G

Y

Y

H

N

V

V

T

K

N

D

P

P

E

Q

R

R

F

F

G
|
G

V

V

E

E

F

F

D

D

N

E

T

K

G

G

R

K

A

V

V

I

S

S

I

I

E

E

E
|
E

K
|
K

P

P

R

E

E

E

P

P

K

K

S

S

H

S

Y

Y

A

A

V
|
V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

Q

R

V

V

I

V

E

D

F

I

A

A

K

K

S

N

L

I

R

T

P

P

S

S

A

A

R
|
R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

I

V

N

N

N

K

A

A

Y

Y

L

L

A

E

A

L

N

G

Q

E

L

L

Q

H

V

V

S

E

V

L

L

L

G
|
G

R
|
R

G
|
G

F

F

A

A

W
|
W

L

L

D

D

T

T

G

G

T

T

H

H

D

E

A

S

L

L

M

L

E

Q

A

A

G

S

H

Q

F

F

V

I

Q

E

T

T

I

I

E

E

K

K

R

R

Q

Q

G

S

L

L

K

K

I

V

A

A

C

C

L

L

E

E

I
|
I

A

A

Y

Y

R

R

N

M

G

G

W

Y

I

I

S

S

T

R

E

E

T

Q

L

L

L

I

Q

K

H

L

A

A

K

E

A

P

L

L

S

M

Q

K

S

N

G

E

Y

Y

G

G

D

Q

Y

Y

L

L

R

M

Q

N

L

L

binding galactose-uridine-5'-diphosphate: L6 (= L20), G8 (= G22), G9 (= G23), Q80 (= Q94), P83 (= P97), G85 (= G99), L86 (≠ I100), L106 (= L120), D108 (= D122), G144 (= G158), E159 (= E173), K160 (= K174), V170 (= V184), R192 (= R206), W221 (= W235)
binding uridine 5'-triphosphate: L43 (= L57), G113 (= G127), H114 (≠ Q128), G115 (≠ S129), T117 (≠ S131), G216 (= G230), R217 (= R231), G218 (= G232), I254 (= I268)

4ho6A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with udp-glucose and utp
68% identity, 93% coverage: 16:299/305 of query aligns to 2:285/288 of 4ho6A

query
sites
4ho6A

K

K

G

G

I

I

L
|
L

A

S

G
|
G

T

S

G

G

S

T

R

R

L

L

Y

Y

P

P

I

L

T

T

R

K

G

V

V

V

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

V

V

Y

Y

P

P

L

L

S

S

V

V

L

L

M

M

L
|
L

A

A

G

G

I

I

R

K

D

D

I

I

L

L

L

I

I

I

S

S

T

T

A

P

S

E

D

D

L

T

A

P

G

R

F

F

Q

E

S

Q

L

L

G

G

D

G

G

G

S

S

G

E

L

L

G

G

I

I

R

S

I

L

S

S

Y

Y

A

A

V

V

Q
|
Q

S

S

K

S

P
|
P

E

D

G
|
G

I

L

A

A

Q

Q

A

A

F

F

L

I

I

I

G

G

E

E

D

E

F

F

I

I

G

G

N

D

D

D

K

N

V

V

A

A

L

L

I

V

L

L

G

G

D
|
D

N

N

I

I

F

F

Y
|
Y

G
|
G

Q
x
H

S
x
G

F

F

S
x
T

R

E

Q

L

L

L

Q

Q

Q

R

A

A

D

N

C

R

S

K

L

S

G

G

A

A

T

T

V

I

F

F

A

G

Y

Y

H

N

V

V

T

K

N

D

P

P

E

Q

R

R

F

F

G
|
G

V

V

E

E

F

F

D

D

N

E

T

K

G

G

R

K

A

V

V

I

S

S

I

I

E

E

E
|
E

K
|
K

P

P

R

E

E

E

P

P

K

K

S

S

H

S

Y

Y

A

A

V
|
V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

Q

R

V

V

I

V

E

D

F

I

A

A

K

K

S

N

L

I

R

T

P

P

S

S

A

A

R
|
R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

I

V

N

N

N

K

A

A

Y

Y

L

L

A

E

A

L

N

G

Q

E

L

L

Q

H

V

V

S

E

V

L

L

L

G

G

R

R

G

G

F

F

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

E

A

S

L

L

M

L

E

Q

A

A

G

S

H

Q

F

F

V

I

Q

E

T

T

I

I

E

E

K

K

R

R

Q

Q

G

S

L

L

K

K

I

V

A

A

C

C

L

L

E

E

E
|
E

I
|
I

A

A

Y

Y

R

R

N

M

G

G

W

Y

I

I

S

S

T

R

E

E

T

Q

L

L

L

I

Q

K

H

L

A

A

K

E

A

P

L

L

S

M

Q

K

S

N

G

E

Y

Y

G

G

D

Q

Y

Y

L

L

R

M

Q

N

L

L

binding uridine-5'-diphosphate-glucose: L6 (= L20), G8 (= G22), G9 (= G23), Q80 (= Q94), P83 (= P97), G85 (= G99), D108 (= D122), G144 (= G158), E159 (= E173), K160 (= K174), V170 (= V184), R192 (= R206)
binding uridine 5'-triphosphate: L43 (= L57), Y112 (= Y126), G113 (= G127), H114 (≠ Q128), G115 (≠ S129), T117 (≠ S131), E253 (= E267), I254 (= I268)

4ho5A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with tdp-glucose
68% identity, 93% coverage: 16:299/305 of query aligns to 2:285/288 of 4ho5A

query
sites
4ho5A

K

K

G

G

I

I

L
|
L

A

S

G
|
G

T

S

G

G

S

T

R

R

L

L

Y

Y

P

P

I

L

T

T

R

K

G

V

V

V

S

S

K

K

Q
|
Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

V

V

Y

Y

P

P

L

L

S

S

V

V

L

L

M

M

L

L

A

A

G

G

I

I

R

K

D

D

I

I

L

L

L

I

I

I

S

S

T

T

A

P

S

E

D

D

L

T

A

P

G

R

F

F

Q

E

S

Q

L

L

G

G

D

G

G

G

S

S

G

E

L

L

G

G

I

I

R

S

I

L

S

S

Y

Y

A

A

V

V

Q
|
Q

S

S

K

S

P
|
P

E

D

G
|
G

I
x
L

A

A

Q

Q

A

A

F

F

L

I

I

I

G

G

E

E

D

E

F

F

I

I

G

G

N

D

D

D

K

N

V

V

A

A

L

L

I

V

L
|
L

G

G

D
|
D

N

N

I

I

F

F

Y
|
Y

G
|
G

Q
x
H

S
x
G

F
|
F

S
x
T

R

E

Q

L

L

L

Q

Q

Q

R

A

A

D

N

C

R

S

K

L

S

G

G

A

A

T

T

V

I

F

F

A

G

Y

Y

H

N

V

V

T

K

N

D

P

P

E

Q

R

R

F
|
F

G
|
G

V

V

E

E

F

F

D

D

N

E

T

K

G

G

R

K

A

V

V

I

S

S

I

I

E

E

E
|
E

K
|
K

P

P

R

E

E

E

P

P

K

K

S

S

H

S

Y

Y

A

A

V
|
V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

Q

R

V

V

I

V

E

D

F

I

A

A

K

K

S

N

L

I

R

T

P

P

S

S

A

A

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

I

V

N

N

N

K

A

A

Y

Y

L

L

A

E

A

L

N

G

Q

E

L

L

Q

H

V

V

S

E

V

L

L

L

G
|
G

R
|
R

G
|
G

F

F

A

A

W
|
W

L

L

D

D

T

T

G

G

T

T

H

H

D

E

A

S

L

L

M

L

E

Q

A

A

G

S

H

Q

F

F

V

I

Q

E

T

T

I

I

E

E

K

K

R

R

Q

Q

G

S

L

L

K

K

I

V

A

A

C

C

L

L

E

E

E
|
E

I
|
I

A

A

Y

Y

R

R

N

M

G

G

W

Y

I

I

S

S

T

R

E

E

T

Q

L

L

L

I

Q

K

H

L

A

A

K

E

A

P

L

L

S

M

Q

K

S

N

G

E

Y

Y

G

G

D

Q

Y

Y

L

L

R

M

Q

N

L

L

binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: L6 (= L20), G8 (= G22), G9 (= G23), Q24 (= Q38), Q80 (= Q94), P83 (= P97), G85 (= G99), L86 (≠ I100), L106 (= L120), D108 (= D122), Y112 (= Y126), G113 (= G127), H114 (≠ Q128), G115 (≠ S129), F116 (= F130), T117 (≠ S131), F143 (= F157), G144 (= G158), E159 (= E173), K160 (= K174), V170 (= V184), G216 (= G230), R217 (= R231), G218 (= G232), W221 (= W235), E253 (= E267), I254 (= I268)

4ho3A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with thymidine triphosphate
68% identity, 93% coverage: 16:299/305 of query aligns to 2:285/288 of 4ho3A

query
sites
4ho3A

K

K

G

G

I

I

L
|
L

A

S

G
|
G

T

S

G
|
G

S
x
T

R
|
R

L

L

Y

Y

P

P

I

L

T

T

R

K

G

V

V

V

S

S

K

K

Q
|
Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

V

V

Y

Y

P

P

L

L

S

S

V

V

L

L

M

M

L
|
L

A

A

G

G

I

I

R

K

D

D

I

I

L

L

L

I

I

I

S

S

T

T

A

P

S

E

D

D

L

T

A

P

G

R

F

F

Q

E

S

Q

L

L

G

G

D

G

G

G

S

S

G

E

L

L

G

G

I

I

R

S

I

L

S

S

Y

Y

A

A

V

V

Q
|
Q

S

S

K

S

P
|
P

E

D

G
|
G

I
x
L

A

A

Q

Q

A

A

F

F

L

I

I

I

G

G

E

E

D

E

F

F

I

I

G

G

N

D

D

D

K

N

V

V

A

A

L

L

I

V

L

L

G

G

D
|
D

N

N

I

I

F

F

Y
|
Y

G
|
G

Q
x
H

S
x
G

F
|
F

S
x
T

R

E

Q

L

L

L

Q

Q

Q

R

A

A

D

N

C

R

S

K

L

S

G

G

A

A

T

T

V

I

F

F

A

G

Y

Y

H

N

V

V

T

K

N

D

P

P

E

Q

R

R

F

F

G

G

V

V

E

E

F

F

D

D

N

E

T

K

G

G

R

K

A

V

V

I

S

S

I

I

E

E

K

K

P

P

R

E

E

E

P

P

K

K

S

S

H

S

Y

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

Q

R

V

V

I

V

E

D

F

I

A

A

K

K

S

N

L

I

R

T

P

P

S

S

A

A

R

R

G

G

E
|
E

L

L

E

E

I

I

T

T

D

D

I

V

N

N

N

K

A

A

Y

Y

L

L

A

E

A

L

N

G

Q

E

L

L

Q

H

V

V

S

E

V

L

L

L

G

G

R

R

G

G

F

F

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

E

A

S

L

L

M

L

E

Q

A

A

G

S

H

Q

F

F

V

I

Q

E

T

T

I

I

E

E

K

K

R

R

Q

Q

G

S

L

L

K

K

I

V

A

A

C

C

L

L

E

E

E
|
E

I
|
I

A

A

Y

Y

R
|
R

N

M

G

G

W

Y

I

I

S

S

T

R

E

E

T

Q

L

L

L

I

Q

K

H

L

A

A

K

E

A

P

L

L

S

M

Q

K

S

N

G

E

Y

Y

G

G

D

Q

Y

Y

L

L

R

M

Q

N

L

L

binding thymidine-5'-triphosphate: L6 (= L20), G8 (= G22), G9 (= G23), G11 (= G25), T12 (≠ S26), R13 (= R27), Q24 (= Q38), L43 (= L57), Q80 (= Q94), P83 (= P97), G85 (= G99), L86 (≠ I100), D108 (= D122), Y112 (= Y126), G113 (= G127), H114 (≠ Q128), G115 (≠ S129), F116 (= F130), T117 (≠ S131), E194 (= E208), E253 (= E267), I254 (= I268), R257 (= R271)

4ho4A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with thymidine and glucose-1-phosphate
68% identity, 93% coverage: 16:299/305 of query aligns to 2:285/289 of 4ho4A

query
sites
4ho4A

K

K

G

G

I

I

L

L

A

S

G

G

T

S

G

G

S

T

R

R

L

L

Y

Y

P

P

I

L

T

T

R

K

G

V

V

V

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

V

V

Y

Y

P

P

L

L

S

S

V

V

L

L

M

M

L

L

A

A

G

G

I

I

R

K

D

D

I

I

L

L

L

I

I

I

S

S

T

T

A

P

S

E

D

D

L

T

A

P

G

R

F

F

Q

E

S

Q

L

L

G

G

D

G

G

G

S

S

G

E

L

L

G

G

I

I

R

S

I

L

S

S

Y

Y

A

A

V

V

Q

Q

S

S

K

S

P

P

E

D

G

G

I

L

A

A

Q

Q

A

A

F

F

L

I

I

I

G

G

E

E

D

E

F

F

I

I

G

G

N

D

D

D

K

N

V

V

A

A

L

L

I

V

L
|
L

G

G

D

D

N

N

I

I

F

F

Y

Y

G

G

Q

H

S

G

F

F

S

T

R

E

Q

L

L

L

Q

Q

Q

R

A

A

D

N

C

R

S

K

L

S

G

G

A

A

T

T

V

I

F

F

A

G

Y

Y

H

N

V

V

T

K

N

D

P

P

E

Q

R

R

F
|
F

G
|
G

V

V

E

E

F

F

D

D

N

E

T

K

G

G

R

K

A

V

V

I

S

S

I

I

E

E

E
|
E

K
|
K

P

P

R

E

E

E

P

P

K

K

S

S

H

S

Y

Y

A

A

V
|
V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

Q

R

V

V

I

V

E

D

F

I

A

A

K

K

S

N

L

I

R

T

P

P

S

S

A

A

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

I

V

N

N

N

K

A

A

Y

Y

L

L

A

E

A

L

N

G

Q

E

L

L

Q

H

V

V

S

E

V

L

L

L

G

G

R

R

G

G

F

F

A

A

W
|
W

L

L

D

D

T

T

G

G

T

T

H

H

D

E

A

S

L

L

M

L

E

Q

A

A

G

S

H

Q

F

F

V

I

Q

E

T

T

I

I

E

E

K

K

R

R

Q

Q

G

S

L

L

K

K

I

V

A

A

C

C

L

L

E

E

I

I

A

A

Y

Y

R

R

N

M

G

G

W

Y

I

I

S

S

T

R

E

E

T

Q

L

L

L

I

Q

K

H

L

A

A

K

E

A

P

L

L

S

M

Q

K

S

N

G

E

Y

Y

G

G

D

Q

Y

Y

L

L

R

M

Q

N

L

L

binding 1-O-phosphono-alpha-D-glucopyranose: L106 (= L120), F143 (= F157), G144 (= G158), E159 (= E173), K160 (= K174), V170 (= V184), W221 (= W235)

5ifyA Crystal structure of glucose-1-phosphate thymidylyltransferase from burkholderia vietnamiensis in complex with 2 -deoxyuridine-5'- monophosphate and 2'-deoxy-thymidine-b-l-rhamnose
65% identity, 93% coverage: 16:300/305 of query aligns to 3:287/293 of 5ifyA

query
sites
5ifyA

K

K

G

G

I

I

L
|
L

A

A

G
|
G

G

G

T

S

G

G

S

T

R

R

L

L

Y

Y

P

P

I

I

T

T

R

H

G

A

V

V

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

V

I

Y

Y

P

P

L

L

S

S

V

T

L

L

M

M

L

I

A

A

G

G

I

I

R

R

D

D

I

V

L

L

L

I

I

I

S

S

T

T

A

P

S

Q

D

D

L

T

A

P

G

R

F

F

Q

E

S

S

L

M

L

L

G

G

D

D

G

G

S

S

G

Q

L

W

G

G

I

M

R

N

I

I

S

Q

Y

Y

A

A

V

T

Q
|
Q

S

P

K

S

P
|
P

E

D

G
|
G

I
x
L

A

A

Q

Q

A

A

F

F

L

V

I

I

G

G

E

R

D

D

F

F

I

V

G

G

N

N

D

E

K

P

V

S

A

A

L

L

I

I

L

L

G

G

D
|
D

N

N

I

I

F

F

Y
|
Y

G
|
G

Q
x
H

S
x
D

F

L

S

A

R

K

Q

Q

L

L

Q

E

Q

R

A

A

A

S

D

A

C

K

S

D

L

T

G

G

A

A

T

T

V

V

F

F

A

A

Y

Y

H

H

V

V

T

Q

N

D

P

P

E

E

R

R

F
x
Y

G
|
G

V

V

E

E

F

F

D

D

N

R

T

E

G

F

R

R

A

A

V

I

S

S

I

I

E

E

E
|
E

K
|
K

P

P

R

A

E

K

P

P

K

R

S

S

H

S

Y

Y

A

A

V
|
V

T

T

G

G

L

L

Y
|
Y

F

F

Y

Y

D

D

N

R

Q

Q

V

V

I

C

E

D

F

I

A

A

K

A

S

D

L

I

R

K

P

P

S

S

A

A

R
|
R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

I

V

N

N

N

S

A

R

Y

Y

L

L

A

A

N

G

Q

Q

L

L

Q

D

V

V

S

E

V

L

L

M

G
|
G

R
|
R

G
|
G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

A

S

L

L

M

I

E

E

A

A

G

G

H

T

F

F

V

I

Q

A

T

T

I

L

E

Q

K

K

R

R

Q

Q

G

G

L

L

K

M

I

V

A

A

C

C

L

P

E

E

E
|
E

I
|
I

A

A

Y

Y

R

R

N

R

G

N

W

W

I

I

S

D

T

A

E

E

T

Q

L

V

L

L

Q

K

H

L

A

A

K

Q

A

P

L

L

S

A

Q

K

S

N

G

A

Y

Y

G

G

D

Q

Y

Y

L

L

R

R

Q

N

L

L

V

L

binding 2'-deoxy-thymidine-beta-l-rhamnose: L7 (= L20), G9 (= G22), Q81 (= Q94), P84 (= P97), G86 (= G99), L87 (≠ I100), D109 (= D122), Y144 (≠ F157), G145 (= G158), E160 (= E173), K161 (= K174), V171 (= V184), Y175 (= Y188), R193 (= R206)
binding 2'-deoxyuridine 5'-monophosphate: Y113 (= Y126), G114 (= G127), H115 (≠ Q128), D116 (≠ S129), G217 (= G230), R218 (= R231), G219 (= G232), E254 (= E267), I255 (= I268)

4hocA Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with udp-n- acetylglucosamine
67% identity, 93% coverage: 16:299/305 of query aligns to 2:283/286 of 4hocA

query
sites
4hocA

K

K

G

G

I

I

L
|
L

A

S

G
|
G

T

S

G

G

S

T

R

R

L

L

Y

Y

P

P

I

L

T

T

R

K

G

V

V

V

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

V

V

Y

Y

P

P

L

L

S

S

V

V

L

L

M

M

L

L

A

A

G

G

I

I

R

K

D

D

I

I

L

L

L

I

I

I

S

S

T

T

A

P

S

E

D

D

L

T

A

P

G

R

F

F

Q

E

S

Q

L

L

G

G

D

G

G

G

S

S

G

E

L

L

G

G

I

I

R

S

I

L

S

S

Y

Y

A

A

V

V

Q
|
Q

S

S

K

S

P
|
P

E

D

G
|
G

I
x
L

A

A

Q

Q

A

A

F

F

L

I

I

I

G

G

E

E

D

E

F

F

I

I

G

G

N

D

D

D

K

N

V

V

A

A

L

L

I

V

L

L

G

G

D
|
D

N

N

I

I

F

F

Y

Y

G

G

Q

H

S

G

F

F

S

T

R

E

Q

L

L

L

Q

Q

Q

R

A

A

D

N

C

R

S

K

L

S

G

G

A

A

T

T

V

I

F

F

A

G

Y

Y

H

N

V

V

T

K

N

D

P

P

E

Q

R

R

F

F

G
|
G

V

V

E

E

F

F

D

D

N

E

T

K

G

G

R

K

A

V

V

I

S

S

I

I

E

E

E
|
E

K
|
K

P

P

R

E

E

E

P

P

K

K

S

S

H

S

Y

Y

A

A

V
|
V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

Q

R

V

V

I

V

E

D

F

I

A

A

K

K

S

N

L

I

R

T

P

P

S

S

A

-

R

-

G

G

E

E

L

L

E

E

I

I

T

T

D

D

I

V

N

N

N

K

A

A

Y

Y

L

L

A

E

A

L

N

G

Q

E

L

L

Q

H

V

V

S

E

V

L

L

L

G

G

R

R

G

G

F

F

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

E

A

S

L

L

M

L

E

Q

A

A

G

S

H

Q

F

F

V

I

Q

E

T

T

I

I

E

E

K

K

R

R

Q

Q

G

S

L

L

K

K

I

V

A

A

C

C

L

L

E

E

I

I

A

A

Y

Y

R

R

N

M

G

G

W

Y

I

I

S

S

T

R

E

E

T

Q

L

L

L

I

Q

K

H

L

A

A

K

E

A

P

L

L

S

M

Q

K

S

N

G

E

Y

Y

G

G

D

Q

Y

Y

L

L

R

M

Q

N

L

L

binding uridine-diphosphate-n-acetylglucosamine: L6 (= L20), G8 (= G22), G9 (= G23), Q80 (= Q94), P83 (= P97), G85 (= G99), L86 (≠ I100), D108 (= D122), G144 (= G158), E159 (= E173), K160 (= K174), V170 (= V184)

P61887 Glucose-1-phosphate thymidylyltransferase 2; G1P-TT 2; dTDP-glucose pyrophosphorylase 2; dTDP-glucose synthase 2; EC 2.7.7.24 from Escherichia coli (strain K12) (see paper)
65% identity, 93% coverage: 16:300/305 of query aligns to 2:286/293 of P61887

query
sites
P61887

K

K

G

G

I

I

L

L

A

A

G

G

T

S

G

G

S

T

R

R

L

L

Y

H

P

P

I

I

T

T

R

R

G

G

V

V

S

S

K

K

Q

Q

L

L

P

P

V

I

Y

Y

D

D

K

K

P

P

M

M

V

I

Y

Y

P

P

L

L

S

S

V

V

L

L

M

M

L

L

A

A

G

G

I

I

R

R

D

E

I

I

L

L

L

I

I

I

S

T

T

T

A

P

S

E

D

D

L

K

A

G

G

Y

F

F

Q

Q

S

R

L

L

G

G

D

D

G

G

S

S

G

E

L

F

G

G

I

I

R

Q

I

L

S

E

Y

Y

A

A

V

E

Q

Q

S

P

K

S

P

P

E

D

G

G

I

L

A

A

Q

Q

A

A

F

F

L

I

I

I

G

G

E

E

D

T

F

F

I

L

G

N

N

G

D

E

K

P

V

S

A

C

L

L

I

V

L

L

G

G

D
|
D

N

N

I

I

F

F

Y

F

G

G

Q

Q

S

G

F

F

S

S

R

P

Q

K

L

L

Q

R

Q

H

A

V

A

A

D

A

C

R

S

T

L

E

G

G

A

A

T

T

V

V

F

F

A

G

Y

Y

H

Q

V

V

T

M

N

D

P

P

E

E

R

R

F

F

G

G

V

V

E

E

F

F

D

D

N

D

T

N

G

F

R

R

A

A

V

I

S

S

I

L

E

E

K

K

P

P

R

K

E

Q

P

P

K

K

S

S

H

N

Y

W

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

S

Q

K

V

V

I

V

E

E

F

Y

A

A

K

K

S

Q

L

V

R

K

P

P

S

S

A

E

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

S

I

I

N

N

N

Q

A

M

Y

Y

L

L

A

E

A

A

N

G

Q

N

L

L

Q

T

V

V

S

E

V

L

L

L

G

G

R

R

G

G

F

F

A

A

W

W

L

L

D
|
D

T

T

G

G

T

T

H

H

D

D

A

S

L

L

M

I

E

E

A

A

G

S

H

T

F

F

V

V

Q

Q

T

T

I

V

E

E

K

K

R

R

Q

Q

G

G

L

F

K

K

I

I

A

A

C

C

L

L

E

E

I

I

A

A

Y

W

R

R

N

N

G

G

W

W

I

L

S

D

T

D

E

E

T

G

L

V

L

K

Q

R

H

A

A

A

K

S

A

S

L

L

S

A

Q

K

S

T

G

G

Y

Y

G

G

D

Q

Y

Y

L

L

R

L

Q

E

L

L

V

L

D108 (= D122) binding
D223 (= D237) binding

1mc3A Crystal structure of rffh (see paper)
65% identity, 93% coverage: 16:300/305 of query aligns to 3:287/291 of 1mc3A

query
sites
1mc3A

K

K

G

G

I

I

L
|
L

A

A

G
|
G

T

S

G
|
G

S
x
T

R
|
R

L

L

Y

H

P

P

I

I

T

T

R

R

G

G

V

V

S

S

K
|
K

Q
|
Q

L

L

P

P

V

I

Y

Y

D

D

K

K

P

P

M

M

V

I

Y

Y

P

P

L

L

S

S

V

V

L

L

M

M

L

L

A

A

G

G

I

I

R

R

D

E

I

I

L

L

L

I

I

I

S

T

T

T

A

P

S

E

D

D

L

K

A

G

G

Y

F

F

Q

Q

S

R

L

L

G

G

D

D

G

G

S

S

G

E

L

F

G

G

I

I

R

Q

I

L

S

E

Y

Y

A

A

V

E

Q
|
Q

S

P

K

S

P
|
P

E

D

G

G

I
x
L

A

A

Q

Q

A

A

F

F

L

I

I

I

G

G

E

E

D

T

F

F

I

L

G

N

N

G

D

E

K

P

V

S

A

C

L

L

I

V

L

L

G

G

D
|
D

N

N

I

I

F

F

Y

F

G

G

Q

Q

S

G

F

F

S

S

R

P

Q

K

L

L

Q

R

Q

H

A

V

A

A

D

A

C

R

S

T

L

E

G

G

A

A

T

T

V

V

F

F

A

G

Y

Y

H

Q

V

V

T

M

N

D

P

P

E

E

R

R

F

F

G

G

V

V

E

E

F

F

D

D

N

D

T

N

G

F

R

R

A

A

V

I

S

S

I

L

E

E

K

K

P

P

R

K

E

Q

P

P

K

K

S

S

H

N

Y

W

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

S

Q

K

V

V

I

V

E

E

F

Y

A

A

K

K

S

Q

L

V

R

K

P

P

S

S

A

E

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

S

I

I

N

N

N

Q

A

M

Y

Y

L

L

A

E

A

A

N

G

Q

N

L

L

Q

T

V

V

S

E

V

L

L

L

G

G

R

R

G

G

F

F

A

A

W

W

L

L

D
|
D

T

T

G

G

T

T

H

H

D

D

A

S

L

L

M

I

E

E

A

A

G

S

H

T

F

F

V

V

Q

Q

T

T

I

V

E

E

K

K

R

R

Q

Q

G

G

L

F

K

K

I

I

A

A

C

C

L

L

E

E

I

I

A

A

Y

W

R

R

N

N

G

G

W

W

I

L

S

D

T

D

E

E

T

G

L

V

L

K

Q

R

H

A

A

A

K

S

A

S

L

L

S

A

Q

K

S

T

G

G

Y

Y

G

G

D

Q

Y

Y

L

L

R

L

Q

E

L

L

V

L

binding magnesium ion: D109 (= D122), D224 (= D237)
binding thymidine-5'-triphosphate: L7 (= L20), G9 (= G22), G10 (= G23), G12 (= G25), T13 (≠ S26), R14 (= R27), K24 (= K37), Q25 (= Q38), Q81 (= Q94), P84 (= P97), L87 (≠ I100), D109 (= D122)

6n0uA Crystal structure of a glucose-1-phosphate thymidylyltransferase from burkholderia phymatum bound to 2'-deoxy-thymidine-b-l-rhamnose
64% identity, 94% coverage: 16:302/305 of query aligns to 5:291/295 of 6n0uA

query
sites
6n0uA

K

K

G

G

I

I

L
|
L

A

A

G
|
G

T

S

G

G

S

T

R

R

L

L

Y

Y

P

P

I

I

T

T

R

H

G

V

V

V

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

V

I

Y

Y

P

P

L

L

S

S

V

T

L

L

M

M

L

V

A

A

G

G

I

I

R

R

D

D

I

V

L

L

L

I

I

I

S

S

T

T

A

P

S

Q

D

D

L

T

A

P

G

R

F

F

Q

E

S

A

L

M

L

L

G

G

D

D

G

G

S

S

G

Q

L

W

G

G

I

M

R

N

I

I

S

Q

Y

Y

A

A

V

V

Q
|
Q

S

P

K

S

P
|
P

E

D

G
|
G

I
x
L

A

A

Q

Q

A

A

F

F

L

I

I

I

G

G

E

R

D

E

F

F

I

V

G

G

N

N

D

D

K

P

V

S

A

A

L

L

I

I

L
|
L

G

G

D
|
D

N

N

I

I

F

F

Y

Y

G

G

Q

H

S

D

F

L

S

A

R

K

Q

Q

L

L

Q

E

Q

R

A

A

A

N

D

A

C

R

S

T

L

D

G

G

A

A

T

T

V

V

F

F

A

A

Y

Y

H

H

V

V

T

H

N

D

P

P

E

E

R

R

F
x
Y

G
|
G

V

V

E

E

F

F

D

D

N

K

T

D

G

F

R

R

A

A

V

L

S

S

I

I

E

E

E
|
E

K
|
K

P

P

R

A

E

K

P

P

K

R

S

S

H

N

Y

Y

A

A

V
|
V

T

T

G
|
G

L

L

Y
|
Y

F

F

Y

Y

D

D

N

N

Q

Q

V

V

I

C

E

D

F

I

A

A

K

A

S

D

L

I

R

K

P

P

S

S

A

A

R
|
R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

I

V

N

N

N

S

A

R

Y

Y

L

L

A

S

A

Q

N

K

Q

T

L

L

Q

D

V

V

S

E

V

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

A

S

L

L

M

I

E

E

A

A

G

A

H

T

F

F

V

I

Q

A

T

T

I

L

E

Q

K

K

R

R

Q

Q

G

G

L

L

K

V

I

V

A

A

C

C

L

P

E

E

I

I

A

A

Y

Y

R

R

N

R

G

K

W

W

I

I

S

N

T

A

E

E

T

Q

L

L

Q

A

H

L

A

A

K

R

A

P

L

L

S

S

Q

K

S

N

G

A

Y

Y

G

G

D

Q

Y

Y

L

L

R

Q

Q

N

L

L

V

L

W

T

D

D

binding 2'-deoxy-thymidine-beta-l-rhamnose: L9 (= L20), G11 (= G22), G12 (= G23), Q83 (= Q94), P86 (= P97), G88 (= G99), L89 (≠ I100), L109 (= L120), D111 (= D122), Y146 (≠ F157), G147 (= G158), E162 (= E173), K163 (= K174), V173 (= V184), G175 (= G186), Y177 (= Y188), R195 (= R206)

1lvwA Crystal structure of glucose-1-phosphate thymidylyltransferase, rmla, complex with dtdp
64% identity, 93% coverage: 16:299/305 of query aligns to 5:288/295 of 1lvwA

query
sites
1lvwA

K

K

G

G

I

I

I

V

L
|
L

A

A

G
|
G

G

G

T

S

G

G

S

T

R

R

L

L

Y

Y

P

P

I

I

T

T

R

R

G

A

V

V

S

S

K

K

Q
|
Q

L

L

P

P

V

I

Y

Y

D

D

K

K

P

P

M

M

V

I

Y

Y

P

P

L

L

S

S

V

V

L

L

M

M

L

L

A

A

G

G

I

I

R

R

D

D

I

I

L

L

L

I

I

I

S

S

T

T

A

P

S

R

D

D

L

L

A

P

G

L

F

Y

Q

R

S

D

L

L

G

G

D

D

G

G

S

S

G

Q

L

F

G

G

I

V

R

R

I

F

S

S

Y

Y

A

R

V

V

Q
|
Q

S

E

K

E

P

P

E

R

G
|
G

I

I

A

A

Q

D

A

A

F

F

L

I

I

V

G

G

E

K

D

D

F

F

I

I

G

G

N

D

D

S

K

K

V

V

A

A

L

L

I

V

L
|
L

G

G

D
|
D

N

N

I

V

F

F

Y
|
Y

G
|
G

Q
x
H

S
x
R

F
|
F

S
|
S

R

E

Q

I

L

L

Q

R

A

A

D

S

C

L

S

E

L

D

G

G

A

A

T

V

V

I

F

F

A

G

Y

Y

H

Y

V

V

T

R

N

D

P

P

E

R

R

P

F

F

G

G

V

V

E

E

F

F

D

D

N

S

T

E

G

G

R

R

A

V

V

I

S

S

I

I

E

E

K

K

P

P

R

S

E

R

P

P

K

K

S

S

H

N

Y

Y

A

V

V

V

T

P

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

Q

Q

V

V

I

V

E

E

F

I

A

A

K

R

S

R

L

I

R

E

P

P

S

S

A

D

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

S

I

V

N

N

N

E

A

E

Y

Y

L

L

A

R

A

M

N

G

Q

K

L

L

Q

R

V

V

S

E

V

L

L

M

G

G

R

R

G

G

F

M

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

A

G

L

L

M

L

E

E

A

A

G

S

H

S

F

F

V

I

Q

E

T

T

I

I

E

Q

K

K

R

R

Q

Q

G

G

L

F

K

Y

I
|
I

A

A

C

C

L

L

E

E

I
|
I

A

A

Y

Y

R

N

N

N

G

G

W

W

I

I

S

T

T

R

E

E

T

D

L

V

L

L

Q

E

H

M

A

A

K

E

A

K

L

L

S

E

Q

K

S

T

G

D

Y

Y

G

G

D

K

Y

Y

L

L

R

R

Q

D

L

L

binding thymidine-5'-diphosphate: L9 (= L20), G11 (= G22), Q27 (= Q38), Q83 (= Q94), G88 (= G99), L109 (= L120), D111 (= D122), Y115 (= Y126), G116 (= G127), H117 (≠ Q128), R118 (≠ S129), F119 (= F130), S120 (= S131), I251 (= I262), I257 (= I268)

4b2xB Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
63% identity, 97% coverage: 6:300/305 of query aligns to 3:297/302 of 4b2xB

query
sites
4b2xB

Q

H

H

H

S

H

A

G

F

S

N

M

T

A

Q

M

K

K

T

R

K

K

G

G

I

I

L

L

A

A

G

G

T

S

G

G

S

T

R

R

L

L

Y

H

P

P

I

A

T

T

R

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

V

I

Y

Y

P

P

L

L

S

S

V

T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

D

E

I

I

L

L

L

I

I

I

S

S

T

T

A

P

S

Q

D

D

L

T

A

P

G

R

F

F

Q

Q

S

Q

L

L

G

G

D

D

G

G

S

S

G

N

L

W

G

G

I

L

R

D

I

L

S

Q

Y

Y

A

A

V

V

Q

Q

S

P

K

S

P

P

E

D

G

G

I

L

A

A

Q

Q

A

A

F

F

L

L

I

I

G

G

E

E

D

S

F

F

I

I

G

G

N

N

D

D

K

L

V

S

A

A

L

L

I

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

Y

Y

G

G

Q

H

S

D

F

F

S

H

R

E

Q

L

L

L

Q

G

Q

S

A

A

A

S

D

Q

C

R

S

Q

L

T

G

G

A

A

T

S

V

V

F

F

A

A

Y

Y

H

H

V

V

T

L

N

D

P

P

E

E

R

R

F

Y

G

G

V

V

E

E

F

F

D

D

N

Q

T

G

G

G

R

K

A

A

V

I

S

S

I

L

E

E

K

K

P

P

R

L

E

E

P

P

K

K

S

S

H

N

Y

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

Q

Q

V

V

I

V

E

D

F

I

A

A

K

R

S

D

L

L

R

K

P

P

S

S

A

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

I

V

N

N

N

R

A

A

Y

Y

L

L

A

E

A

R

N

G

Q

Q

L

L

Q

S

V

V

S

E

V

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

A

S

L

L

M

L

E

E

A

A

G

G

H

Q

F

F

V

I

Q

A

T

T

I

L

E

E

K

N

R

R

Q

Q

G

G

L

L

K

K

I

V

A

A

C

C

L

P

E

E

E
|
E

I

I

A

A

Y

Y

R

R

N
x
Q

G

K

W

W

I

I

S

D

T

A

E

A

T

Q

L

L

L

E

Q

K

H

L

A

A

K

A

A

P

L

L

S

A

Q

K

S

N

G

G

Y

Y

G

G

D

Q

Y

Y

L

L

R

K

Q

R

L

L

V

L

binding 6-amino-1-butyl-5-(cyclopentylamino)pyrimidine-2,4(1H,3H)-dione: L54 (= L57), Y122 (≠ F125), E264 (= E267), Q269 (≠ N272)

4b5bA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
64% identity, 94% coverage: 14:300/305 of query aligns to 2:288/293 of 4b5bA

query
sites
4b5bA

K

K

T

R

K

K

G

G

I

I

L

L

A

A

G

G

T

S

G

G

S

T

R

R

L

L

Y

H

P

P

I

A

T

T

R

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

V

I

Y

Y

P

P

L

L

S
|
S

V

T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

D

E

I

I

L

L

L

I

I

I

S

S

T

T

A

P

S

Q

D

D

L

T

A

P

G

R

F

F

Q

Q

S

Q

L

L

G

G

D

D

G

G

S

S

G

N

L

W

G

G

I

L

R

D

I

L

S

Q

Y

Y

A

A

V

V

Q

Q

S

P

K

S

P

P

E

D

G

G

I

L

A

A

Q

Q

A

A

F

F

L

L

I

I

G

G

E

E

D

S

F

F

I

I

G

G

N

N

D

D

K

L

V

S

A

A

L

L

I

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

Y

Y

G
|
G

Q

H

S

D

F

F

S

H

R

E

Q

L

L

L

Q

G

Q

S

A

A

A

S

D

Q

C

R

S

Q

L

T

G

G

A

A

T

S

V

V

F

F

A

A

Y

Y

H

H

V

V

T

L

N

D

P

P

E

E

R

R

F

Y

G

G

V

V

E

E

F

F

D

D

N

Q

T

G

G

G

R

K

A

A

V

I

S

S

I

L

E

E

K

K

P

P

R

L

E

E

P

P

K

K

S

S

H

N

Y

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

Q

Q

V

V

I

V

E

D

F

I

A

A

K

R

S

D

L

L

R

K

P

P

S

S

A

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

I

V

N

N

N

R

A

A

Y

Y

L

L

A

E

A

R

N

G

Q

Q

L

L

Q

S

V

V

S

E

V

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

A

S

L

L

M

L

E

E

A

A

G

G

H

Q

F

F

V

I

Q

A

T

T

I

L

E

E

K

N

R

R

Q

Q

G

G

L

L

K

K

I
x
V

A
|
A

C

C

L

P

E

E

E
|
E

I
|
I

A

A

Y

Y

R

R

N

Q

G

K

W

W

I

I

S

D

T

A

E

A

T

Q

L

L

L

E

Q

K

H

L

A

A

K

A

A

P

L

L

S

A

Q

K

S

N

G

G

Y

Y

G

G

D

Q

Y

Y

L

L

R

K

Q

R

L

L

V

L

binding N-(6-amino-1-benzyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-N,3-dimethylbenzenesulfonamide: S41 (= S53), L45 (= L57), Y113 (≠ F125), G115 (= G127), V250 (≠ I262), A251 (= A263), E255 (= E267), I256 (= I268)

4b4gA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
64% identity, 94% coverage: 14:300/305 of query aligns to 2:288/293 of 4b4gA

query
sites
4b4gA

K

K

T

R

K

K

G

G

I

I

L

L

A

A

G

G

T

S

G

G

S

T

R

R

L

L

Y

H

P

P

I

A

T

T

R

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

V

I

Y

Y

P

P

L

L

S

S

V

T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

D

E

I

I

L

L

L

I

I

I

S

S

T

T

A

P

S

Q

D

D

L

T

A

P

G

R

F

F

Q

Q

S

Q

L

L

G

G

D

D

G

G

S

S

G

N

L

W

G

G

I

L

R

D

I

L

S

Q

Y

Y

A

A

V

V

Q

Q

S

P

K

S

P

P

E

D

G

G

I

L

A

A

Q

Q

A

A

F

F

L

L

I

I

G

G

E

E

D

S

F

F

I

I

G

G

N

N

D

D

K

L

V

S

A

A

L

L

I

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

Y

Y

G
|
G

Q

H

S

D

F
|
F

S

H

R

E

Q

L

L

L

Q

G

Q

S

A

A

A

S

D

Q

C

R

S

Q

L

T

G

G

A

A

T

S

V

V

F

F

A

A

Y

Y

H

H

V

V

T

L

N

D

P

P

E

E

R

R

F

Y

G

G

V

V

E

E

F

F

D

D

N

Q

T

G

G

G

R

K

A

A

V

I

S

S

I

L

E

E

K

K

P

P

R

L

E

E

P

P

K

K

S

S

H

N

Y

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

Q

Q

V

V

I

V

E

D

F

I

A

A

K

R

S

D

L

L

R

K

P

P

S

S

A

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

I

V

N

N

N

R

A

A

Y

Y

L

L

A

E

A

R

N

G

Q

Q

L

L

Q

S

V

V

S

E

V

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

A

S

L

L

M

L

E

E

A

A

G

G

H

Q

F

F

V

I

Q

A

T

T

I

L

E

E

K

N

R

R

Q

Q

G

G

L

L

K

K

I
x
V

A
|
A

C

C

L

P

E

E

E
|
E

I
|
I

A

A

Y

Y

R
|
R

N

Q

G

K

W

W

I

I

S

D

T

A

E

A

T

Q

L

L

L

E

Q

K

H

L

A

A

K

A

A

P

L

L

S

A

Q

K

S

N

G

G

Y

Y

G

G

D

Q

Y

Y

L

L

R

K

Q

R

L

L

V

L

binding N-(6-amino-1-benzyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylbutane-1-sulfonamide: L45 (= L57), Y113 (≠ F125), G115 (= G127), F118 (= F130), V250 (≠ I262), A251 (= A263), E255 (= E267), I256 (= I268), R259 (= R271)

4b42A Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
64% identity, 94% coverage: 14:300/305 of query aligns to 2:288/293 of 4b42A

query
sites
4b42A

K

K

T

R

K

K

G

G

I

I

L

L

A

A

G

G

T

S

G

G

S

T

R

R

L

L

Y

H

P

P

I

A

T

T

R

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

V

I

Y

Y

P

P

L

L

S
|
S

V
x
T

L

L

M

M

L

L

A

A

G

G

I

I

R

R

D

E

I

I

L

L

L

I

I

I

S

S

T

T

A

P

S

Q

D

D

L

T

A

P

G

R

F

F

Q

Q

S

Q

L

L

G

G

D

D

G

G

S

S

G

N

L

W

G

G

I

L

R

D

I

L

S

Q

Y

Y

A

A

V

V

Q

Q

S

P

K

S

P

P

E

D

G

G

I

L

A

A

Q

Q

A

A

F

F

L

L

I

I

G

G

E

E

D

S

F

F

I

I

G

G

N

N

D

D

K

L

V

S

A

A

L

L

I

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

Y

Y

G

G

Q

H

S

D

F
|
F

S

H

R

E

Q

L

L

L

Q

G

Q

S

A

A

A

S

D

Q

C

R

S

Q

L

T

G

G

A

A

T

S

V

V

F

F

A

A

Y

Y

H

H

V

V

T

L

N

D

P

P

E

E

R

R

F

Y

G

G

V

V

E

E

F

F

D

D

N

Q

T

G

G

G

R

K

A

A

V

I

S

S

I

L

E

E

K

K

P

P

R

L

E

E

P

P

K

K

S

S

H

N

Y

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

Q

Q

V

V

I

V

E

D

F

I

A

A

K

R

S

D

L

L

R

K

P

P

S

S

A

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

I

V

N

N

N

R

A

A

Y

Y

L

L

A

E

A

R

N

G

Q

Q

L

L

Q

S

V

V

S

E

V

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

A

S

L

L

M

L

E

E

A

A

G

G

H

Q

F

F

V

I

Q

A

T

T

I

L

E

E

K

N

R

R

Q

Q

G

G

L

L

K

K

I
x
V

A

A

C

C

L

P

E

E

E
|
E

I
|
I

A

A

Y

Y

R

R

N

Q

G

K

W

W

I

I

S

D

T

A

E

A

T

Q

L

L

L

E

Q

K

H

L

A

A

K

A

A

P

L

L

S

A

Q

K

S

N

G

G

Y

Y

G

G

D

Q

Y

Y

L

L

R

K

Q

R

L

L

V

L

binding N-[6-azanyl-1-butyl-2,4-bis(oxidanylidene)pyrimidin-5-yl]-N-methyl-benzamide: S41 (= S53), T42 (≠ V54), Y113 (≠ F125), F118 (= F130), V250 (≠ I262), E255 (= E267), I256 (= I268)

4b3uA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
64% identity, 94% coverage: 14:300/305 of query aligns to 8:294/299 of 4b3uA

query
sites
4b3uA

K

K

T

R

K

K

G

G

I

I

L

L

A

A

G

G

T

S

G

G

S

T

R

R

L

L

Y

H

P

P

I

A

T

T

R

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

V

I

Y

Y

P

P

L

L

S

S

V

T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

D

E

I

I

L

L

L

I

I

I

S

S

T

T

A

P

S

Q

D

D

L

T

A

P

G

R

F

F

Q

Q

S

Q

L

L

G

G

D

D

G

G

S

S

G

N

L

W

G

G

I

L

R

D

I

L

S

Q

Y

Y

A

A

V

V

Q

Q

S

P

K

S

P

P

E

D

G

G

I

L

A

A

Q

Q

A

A

F

F

L

L

I

I

G

G

E

E

D

S

F

F

I

I

G

G

N

N

D

D

K

L

V

S

A

A

L

L

I

V

L

L

G

G

D

D

N

N

I

L

F

Y

Y

Y

G

G

Q

H

S

D

F

F

S

H

R

E

Q

L

L

L

Q

G

Q

S

A

A

A

S

D

Q

C

R

S

Q

L

T

G

G

A

A

T

S

V

V

F

F

A

A

Y

Y

H

H

V

V

T

L

N

D

P

P

E

E

R

R

F

Y

G

G

V

V

E

E

F

F

D

D

N

Q

T

G

G

G

R

K

A

A

V

I

S

S

I

L

E

E

K

K

P

P

R

L

E

E

P

P

K

K

S

S

H

N

Y

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

Q

Q

V

V

I

V

E

D

F

I

A

A

K

R

S

D

L

L

R

K

P

P

S

S

A

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

I

V

N

N

N

R

A

A

Y

Y

L

L

A

E

A

R

N

G

Q

Q

L

L

Q

S

V

V

S

E

V

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

A

S

L

L

M

L

E

E

A

A

G

G

H

Q

F

F

V

I

Q

A

T

T

I

L

E

E

K

N

R

R

Q

Q

G

G

L

L

K

K

I

V

A

A

C

C

L

P

E

E

E
|
E

I
|
I

A

A

Y

Y

R
|
R

N
x
Q

G

K

W

W

I

I

S

D

T

A

E

A

T

Q

L

L

L

E

Q

K

H

L

A

A

K

A

A

P

L

L

S

A

Q

K

S

N

G

G

Y

Y

G

G

D

Q

Y

Y

L

L

R

K

Q

R

L

L

V

L

binding 6-amino-1-benzyl-5-(ethylamino)pyrimidine-2,4(1H,3H)-dione: L51 (= L57), E261 (= E267), I262 (= I268), R265 (= R271), Q266 (≠ N272)

4asyA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
64% identity, 94% coverage: 14:300/305 of query aligns to 2:288/293 of 4asyA

query
sites
4asyA

K

K

T

R

K

K

G

G

I

I

L

L

A

A

G

G

T

S

G

G

S

T

R

R

L

L

Y

H

P

P

I

A

T

T

R

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

V

I

Y

Y

P

P

L

L

S
|
S

V
x
T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

D

E

I

I

L

L

L

I

I

I

S

S

T

T

A

P

S

Q

D

D

L

T

A

P

G

R

F

F

Q

Q

S

Q

L

L

G

G

D

D

G

G

S

S

G

N

L

W

G

G

I

L

R

D

I

L

S

Q

Y

Y

A

A

V

V

Q

Q

S

P

K

S

P

P

E

D

G

G

I

L

A

A

Q

Q

A

A

F

F

L

L

I

I

G

G

E

E

D

S

F

F

I

I

G

G

N

N

D

D

K

L

V

S

A

A

L

L

I

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

Y

Y

G
|
G

Q

H

S

D

F

F

S

H

R

E

Q

L

L

L

Q

G

Q

S

A

A

A

S

D

Q

C

R

S

Q

L

T

G

G

A

A

T

S

V

V

F

F

A

A

Y

Y

H

H

V

V

T

L

N

D

P

P

E

E

R

R

F

Y

G

G

V

V

E

E

F

F

D

D

N

Q

T

G

G

G

R

K

A

A

V

I

S

S

I

L

E

E

K

K

P

P

R

L

E

E

P

P

K

K

S

S

H

N

Y

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

Q

Q

V

V

I

V

E

D

F

I

A

A

K

R

S

D

L

L

R

K

P

P

S

S

A

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

I

V

N

N

N

R

A

A

Y

Y

L

L

A

E

A

R

N

G

Q

Q

L

L

Q

S

V

V

S

E

V

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

A

S

L

L

M

L

E

E

A

A

G

G

H

Q

F

F

V

I

Q

A

T

T

I

L

E

E

K

N

R

R

Q

Q

G

G

L

L

K

K

I
x
V

A

A

C

C

L

P

E

E

E
|
E

I

I

A

A

Y

Y

R

R

N

Q

G

K

W

W

I

I

S

D

T

A

E

A

T

Q

L

L

L

E

Q

K

H

L

A

A

K

A

A

P

L

L

S

A

Q

K

S

N

G

G

Y

Y

G

G

D

Q

Y

Y

L

L

R

K

Q

R

L

L

V

L

binding N-(6-amino-1-benzyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylbenzamide: S41 (= S53), T42 (≠ V54), L45 (= L57), Y113 (≠ F125), G115 (= G127), V250 (≠ I262), E255 (= E267)

5fyeA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor
64% identity, 94% coverage: 13:300/305 of query aligns to 4:291/296 of 5fyeA

query
sites
5fyeA

Q

H

K

K

T

R

K

K

G

G

I

I

L

L

A

A

G

G

T

S

G

G

S

T

R

R

L

L

Y

H

P

P

I

A

T

T

R

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

V

I

Y

Y

P

P

L

L

S
|
S

V

T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

D

E

I

I

L

L

L

I

I

I

S

S

T

T

A

P

S

Q

D

D

L

T

A

P

G

R

F

F

Q

Q

S

Q

L

L

G

G

D

D

G

G

S

S

G

N

L

W

G

G

I

L

R

D

I

L

S

Q

Y

Y

A

A

V

V

Q

Q

S

P

K

S

P

P

E

D

G

G

I

L

A

A

Q

Q

A

A

F

F

L

L

I

I

G

G

E

E

D

S

F

F

I

I

G

G

N

N

D

D

K

L

V

S

A

A

L

L

I

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

Y

Y

G
|
G

Q

H

S

D

F

F

S

H

R

E

Q

L

L

L

Q

G

Q

S

A

A

A

S

D

Q

C

R

S

Q

L

T

G

G

A

A

T

S

V

V

F

F

A

A

Y

Y

H

H

V

V

T

L

N

D

P

P

E

E

R

R

F

Y

G

G

V

V

E

E

F

F

D

D

N

Q

T

G

G

G

R

K

A

A

V

I

S

S

I

L

E

E

K

K

P

P

R

L

E

E

P

P

K

K

S

S

H

N

Y

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

Q

Q

V

V

I

V

E

D

F

I

A

A

K

R

S

D

L

L

R

K

P

P

S

S

A

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

I

V

N

N

N

R

A

A

Y

Y

L

L

A

E

A

R

N

G

Q

Q

L

L

Q

S

V

V

S

E

V

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

A

S

L

L

M

L

E

E

A

A

G

G

H

Q

F

F

V

I

Q

A

T

T

I

L

E

E

K

N

R

R

Q

Q

G

G

L

L

K

K

I
x
V

A
|
A

C

C

L

P

E

E

E
|
E

I

I

A

A

Y

Y

R
|
R

N

Q

G

K

W

W

I

I

S

D

T

A

E

A

T

Q

L

L

L

E

Q

K

H

L

A

A

K

A

A

P

L

L

S

A

Q

K

S

N

G

G

Y

Y

G

G

D

Q

Y

Y

L

L

R

K

Q

R

L

L

V

L

binding N-(6-Amino-1-(3-fluorobenzyl)-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylbenzenesulfonamide: S44 (= S53), L48 (= L57), Y116 (≠ F125), G118 (= G127), V253 (≠ I262), A254 (= A263), E258 (= E267), R262 (= R271)

5fu0A Pseudomonas aeruginosa rmla in complex with allosteric inhibitor
64% identity, 94% coverage: 13:300/305 of query aligns to 4:291/296 of 5fu0A

query
sites
5fu0A

Q

H

K

K

T

R

K

K

G

G

I

I

L

L

A

A

G

G

T

S

G

G

S

T

R

R

L

L

Y

H

P

P

I

A

T

T

R

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

V

I

Y

Y

P

P

L

L

S
|
S

V

T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

D

E

I

I

L

L

L

I

I

I

S

S

T

T

A

P

S

Q

D

D

L

T

A

P

G

R

F

F

Q

Q

S

Q

L

L

G

G

D

D

G

G

S

S

G

N

L

W

G

G

I

L

R

D

I

L

S

Q

Y

Y

A

A

V

V

Q

Q

S

P

K

S

P

P

E

D

G

G

I

L

A

A

Q

Q

A

A

F

F

L

L

I

I

G

G

E

E

D

S

F

F

I

I

G

G

N

N

D

D

K

L

V

S

A

A

L

L

I

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

Y
|
Y

G
|
G

Q

H

S

D

F

F

S
x
H

R

E

Q

L

L

L

Q

G

Q

S

A

A

A

S

D

Q

C

R

S

Q

L

T

G

G

A

A

T

S

V

V

F

F

A

A

Y

Y

H

H

V

V

T

L

N

D

P

P

E

E

R

R

F

Y

G

G

V

V

E

E

F

F

D

D

N

Q

T

G

G

G

R

K

A

A

V

I

S

S

I

L

E

E

K

K

P

P

R

L

E

E

P

P

K

K

S

S

H

N

Y

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

Q

Q

V

V

I

V

E

D

F

I

A

A

K

R

S

D

L

L

R

K

P

P

S

S

A

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

I

V

N

N

N

R

A

A

Y

Y

L

L

A

E

A

R

N

G

Q

Q

L

L

Q

S

V

V

S

E

V

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

A

S

L

L

M

L

E

E

A

A

G

G

H

Q

F

F

V

I

Q

A

T

T

I

L

E

E

K

N

R

R

Q

Q

G

G

L

L

K

K

I
x
V

A
|
A

C

C

L

P

E

E

E
|
E

I

I

A

A

Y

Y

R

R

N
x
Q

G

K

W

W

I

I

S

D

T

A

E

A

T

Q

L

L

L

E

Q

K

H

L

A

A

K

A

A

P

L

L

S

A

Q

K

S

N

G

G

Y

Y

G

G

D

Q

Y

Y

L

L

R

K

Q

R

L

L

V

L

binding n-(6-amino-1-(3-methylbenzyl)-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-n-methylbenzenesulfonamide: S44 (= S53), L48 (= L57), Y116 (≠ F125), Y117 (= Y126), G118 (= G127), H122 (≠ S131), V253 (≠ I262), A254 (= A263), E258 (= E267), Q263 (≠ N272)

5ftvA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor
64% identity, 94% coverage: 13:300/305 of query aligns to 4:291/296 of 5ftvA

query
sites
5ftvA

Q

H

K

K

T

R

K

K

G

G

I

I

L

L

A

A

G

G

T

S

G

G

S

T

R

R

L

L

Y

H

P

P

I

A

T

T

R

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

V

I

Y

Y

P

P

L

L

S
|
S

V

T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

D

E

I

I

L

L

L

I

I

I

S

S

T

T

A

P

S

Q

D

D

L

T

A

P

G

R

F

F

Q

Q

S

Q

L

L

G

G

D

D

G

G

S

S

G

N

L

W

G

G

I

L

R

D

I

L

S

Q

Y

Y

A

A

V

V

Q

Q

S

P

K

S

P

P

E

D

G

G

I

L

A

A

Q

Q

A

A

F

F

L

L

I

I

G

G

E

E

D

S

F

F

I

I

G

G

N

N

D

D

K

L

V

S

A

A

L

L

I

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

Y
|
Y

G
|
G

Q

H

S

D

F
|
F

S

H

R

E

Q

L

L

L

Q

G

Q

S

A

A

A

S

D

Q

C

R

S

Q

L

T

G

G

A

A

T

S

V

V

F

F

A

A

Y

Y

H

H

V

V

T

L

N

D

P

P

E

E

R

R

F

Y

G

G

V

V

E

E

F

F

D

D

N

Q

T

G

G

G

R

K

A

A

V

I

S

S

I

L

E

E

K

K

P

P

R

L

E

E

P

P

K

K

S

S

H

N

Y

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

Q

Q

V

V

I

V

E

D

F

I

A

A

K

R

S

D

L

L

R

K

P

P

S

S

A

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

I

V

N

N

N

R

A

A

Y

Y

L

L

A

E

A

R

N

G

Q

Q

L

L

Q

S

V

V

S

E

V

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

A

S

L

L

M

L

E

E

A

A

G

G

H

Q

F

F

V

I

Q

A

T

T

I

L

E

E

K

N

R

R

Q

Q

G

G

L

L

K

K

I
x
V

A
|
A

C

C

L

P

E

E

E
|
E

I

I

A

A

Y

Y

R
|
R

N
x
Q

G

K

W

W

I

I

S

D

T

A

E

A

T

Q

L

L

L

E

Q

K

H

L

A

A

K

A

A

P

L

L

S

A

Q

K

S

N

G

G

Y

Y

G

G

D

Q

Y

Y

L

L

R

K

Q

R

L

L

V

L

binding n-(6-amino-1-(4-bromobenzyl)-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-n-methylbenzenesulfonamide: S44 (= S53), L48 (= L57), Y116 (≠ F125), Y117 (= Y126), G118 (= G127), F121 (= F130), V253 (≠ I262), A254 (= A263), E258 (= E267), R262 (= R271), Q263 (≠ N272)

Query Sequence

>6938119 FitnessBrowser__SB2B:6938119
MSLNIQHSAFNTQKTKGIILAGGTGSRLYPITRGVSKQLLPVYDKPMVYYPLSVLMLAGI
RDILLISTASDLAGFQSLLGDGSGLGIRISYAVQSKPEGIAQAFLIGEDFIGNDKVALIL
GDNIFYGQSFSRQLQQAADCSLGATVFAYHVTNPERFGVVEFDNTGRAVSIEEKPREPKS
HYAVTGLYFYDNQVIEFAKSLRPSARGELEITDINNAYLAANQLQVSVLGRGFAWLDTGT
HDALMEAGHFVQTIEKRQGLKIACLEEIAYRNGWISTETLLQHAKALSQSGYGDYLRQLV
WDASL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory