SitesBLAST

5bjvA X-ray structure of the pglf udp-n-acetylglucosamine 4,6-dehydratase from campylobacterjejuni, d396n/k397a variant in complex with udp-n- acrtylglucosamine (see paper)
45% identity, 55% coverage: 258:610/644 of query aligns to 2:331/339 of 5bjvA

query
sites
5bjvA

I

I

E

E

D

D

L

L

G

A

R

R

D

K

A

P

V

K

A

D

P

L

R

D

K

D

D

S

L

A

M

V

A

A

N

F

I

L

T

K

G

D

K

K

V

V

M

L

V

V

T

S

G
|
G

A

A

G

G

G
|
G

S
x
T

I
|
I

G

G

S

S

E

E

L

L

C

C

R

K

Q

Q

I

C

L

I

R

K

L

F

A

G

P

A

R

K

K

H

L

L

V

I

L

M

F

V

E
x
D

L
x
H

S

S

E

E

F

Y

G

N

L

L

Y

Y

S

K

I

I

E

N

R

D

E

D

L

L

Q

N

A

L

I

Y

R

K

A

E

E

K

L

I

G

T

L

-

D

-

V

-

E

-

L

-

F

-

P

P

M

I

M

L

G

L

S
|
S

V
x
I

Q

L

R

D

Q

K

N

Q

R

S

V

L

Q

D

A

E

V

V

M

L

E

K

A

T

F

Y

K

K

V

P

Q

E

T

L

V

I

Y

L

H

H

A
|
A

Y

Y

K
|
K

H
|
H

V

V

P

P

L

L

V

C

E

E

H

Q

N

N

V

P

V

H

E

S

G

A

V

V

R

I

N
|
N

N

N

V

I

F

L

G

G

T

T

L

K

Y

I

T

L

A

C

Q

D

A

S

A

A

I

K

A

E

T

N

G

K

V

V

E

A

T

K

F

F

V

V

L

M

I
|
I

S

S

T
|
T

D

N

K

A

A

A

V

V

R

R

P

P

T

T

N

N

V

I

M

M

G

G

T

C

T

T

K
|
K

R

R

M

V

A

C

E

E

L

L

V

Y

L

T

Q

L

A

S

L

M

S

S

K

D

L

E

N

N

S

-

G

-

T

F

R

E

F

V

C

A

M

C

V

V

R

R

F
|
F

G
|
G

N
|
N

V
|
V

L

L

G

G

S
|
S

G

G

S
|
S

V
|
V

V

I

P

P

L

K

F

F

R

K

K

A

Q

Q

I

I

A

A

N

N

G

N

G

E

P

P

V

L

T
|
T

V
x
L

T
|
T

H

H

P

P

E

D

I
|
I

T

V

R
|
R

F

Y

F

F

M

M

T

L

I

V

P

A

E

E

A

A

S

V

Q

Q

L

L

V

V

I

L

Q

Q

A

A

G

G

A

A

M

I

G

A

I

K

G

G

D

E

V

L

F

F

V

V

L

L

D

D

M

M

G

G

Q

K

S

P

V

V

K

K

I

I

V

I

D

D

L

L

A

A

R

K

K

K

M

M

I

L

R

L

L

L

S

S

G

N

F

-

E

-

V

-

K

-

D

-

E

-

H

-

N

-

P

-

D

R

G

N

D

D

I

L

E

E

I

I

Q

K

F

I

S

T

G

G

L

L

R
|
R

P

K

G

G

E
|
E

K

K

L

L

Y

Y

E

E

L

L

I

I

G

D

D

E

N

N

V

D

E

A

G

K

T

T

G

Q

H

Y

E

E

R

S

I

I

M

F

T

V

A

A

-

K

-

N

H

E

E

K

V

V

F

D

L

L

T

D

W

W

E

-

L

L

K

N

P

K

V

E

L

I

D

E

K

N

L

L

D

Q

M

I

A

C

C

-

H

-

A

-

F

-

D

-

H

-

E

E

S

D

I

I

R

S

E

E

L

A

M

L

L

L

K

K

binding nicotinamide-adenine-dinucleotide: G31 (= G287), G34 (= G290), T35 (≠ S291), I36 (= I292), D56 (≠ E312), H57 (≠ L313), S82 (= S344), I83 (≠ V345), A104 (= A366), A105 (= A367), A106 (= A368), K108 (= K370), N123 (= N385), I146 (= I408), K162 (= K424), F184 (= F448), G185 (= G449), N186 (= N450), V187 (= V451), S190 (= S454), S191 (= S455)
binding uridine-diphosphate-n-acetylglucosamine: K108 (= K370), H109 (= H371), T148 (= T410), G185 (= G449), N186 (= N450), S193 (= S457), V194 (= V458), T209 (= T473), L210 (≠ V474), T211 (= T475), I215 (= I479), R217 (= R481), R276 (= R549), E279 (= E552)

6bwcC X-ray structure of pen from bacillus thuringiensis (see paper)
36% identity, 44% coverage: 281:562/644 of query aligns to 6:275/327 of 6bwcC

query
sites
6bwcC

K

K

V

N

V

V

M

L

V

I

T

I

G
|
G

A

G

G
x
T

G
|
G

S
x
T

I
|
I

G

G

S

K

E

S

L

I

C

L

R

S

Q

N

I

V

L

L

R

Q

L

E

A

K

P

P

R

K

K

V

L

V

V

R

L

V

F

F

E
x
S

L
x
R

S
|
S

E

E

F

Y

G

N

L

Q

Y

F

S

L

I

L

E

Q

R

E

E

E

L

F

Q

R

A

D

I

K

R

N

A

R

E

N

L

I

G

R

L

Y

D

-

V

-

E

-

L

-

F

-

P

-

M

L

M

I

G

G

S
x
D

V
x
I

Q

R

R

N

Q

Y

N

D

R

R

V

V

Q

F

A

S

V

A

M

M

E

E

A

-

F

-

K

N

V

I

Q

D

T

Y

V

V

Y

F

H

H

A
x
V

A
|
A

A

A

Y

M

K
|
K

H
|
H

V

V

P

S

L

F

V

C

E

E

H

Y

N

N

V

P

V

F

E

E

G

A

V

V

R

L

N

T

N

N

V

I

F

F

G

G

T

T

L

Q

Y

N

T

V

A

I

Q

K

A

A

I

I

A

A

T

Q

G

K

V

V

E

K

T

K

F

V

V

V

L

F

I
x
T

S

S

T
x
S

D
x
N

K

A

A

A

V

I

R

S

P

P

T

T

N

N

V

N

M
x
Y

G

G

T

A

T

T

K
|
K

R

L

M

T

A

A

E

E

L

R

V

L

L

I

Q

T

A

S

-

A

-

E

L

Y

S

S

K

K

L

G

N

S

S

S

G

E

T

T

R

T

F

F

C

T

M

S

V

V

R

R

F
|
F

G

G

N
|
N

V
|
V

L

M

G

G

S
|
S

S
x
R

G

G

S
|
S

V
|
V

V

I

P

P

L
|
L

F

F

R

E

K

N

Q

Q

I

I

A

K

N

E

G

N

G

Q

P

K

V

I

T
|
T

V

V

T
|
T

H

D

P

L

E

S

I
x
M

T

S

R
|
R

F

F

F

M

M

M

T

T

I

L

P

N

E

Q

A

A

S

T

Q

M

L

L

V

T

I

I

Q

E

A

A

G

M

A

K

M

I

G

A

I

K

G

G

D

E

V

T

F

F

V

I

L

L

D

K

M

M

G

-

Q

P

S

V

V

I

K

S

I
x
L

V

N

D

D

L

L

A

S

R

E

K

V

M

M

I

I

-

E

-

V

-

T

R

K

L

L

S

Y

G

G

F

L

E

E

V

-

K

-

D

-

E

-

H

-

N

-

P

-

D

-

G

-

D

N

I

I

E

K

I

I

Q

E

F

E

S

I

G

G

L

L

R

K

P

P

G

G

E
|
E

K

K

L

M

Y

Y

E

E

L

L

L

M

I

T

G

H

D

D

binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G287), T14 (≠ G289), G15 (= G290), T16 (≠ S291), I17 (= I292), S37 (≠ E312), R38 (≠ L313), S39 (= S314), D63 (≠ S344), I64 (≠ V345), V83 (≠ A366), A84 (= A367), K87 (= K370), T125 (≠ I408), S127 (≠ T410), Y137 (≠ M420), K141 (= K424), F167 (= F448), V170 (= V451), S173 (= S454), R174 (≠ S455)
binding uridine-diphosphate-n-acetylglucosamine: K87 (= K370), H88 (= H371), S127 (≠ T410), N128 (≠ D411), Y137 (≠ M420), N169 (= N450), S176 (= S457), V177 (= V458), L180 (= L461), T192 (= T473), T194 (= T475), M198 (≠ I479), R200 (= R481), L234 (≠ I516), E265 (= E552)

4j2oC Crystal structure of NADP-bound wbjb from a. Baumannii community strain d1279779 (see paper)
34% identity, 49% coverage: 281:593/644 of query aligns to 5:309/316 of 4j2oC

query
sites
4j2oC

K

K

V

V

V

L

M

L

V

I

T

T

G
|
G

A

G

G
x
T

G
|
G

S
|
S

I
x
F

G

G

S

N

E

A

L

V

C

L

R

K

Q

R

I

F

L

L

R

E

L

T

A

D

P

I

R

K

K

E

L

I

V

R

L

I

F

F

E
x
S

L
x
R

S
x
D

E

E

F

K

G
x
K

L

Q

Y

D

S

D

I

M

E

R

R

K

E

K

L

Y

Q

H

A

S

I

A

R

K

A

L

E

K

L

F

G

-

L

-

D

-

V

-

E

-

L

-

F

-

P

-

M

Y

M

I

G

G

S
x
D

V
|
V

Q

R

R

D

Q

Y

N

N

R

-

V

-

Q

-

A

S

V

I

M

L

E

N

A

A

F

T

K

R

-

G

V

V

Q

D

T

Y

V

I

Y

Y

H

H

A
|
A

Y

L

K
|
K

H

Q

V

V

P

P

L

S

V

C

E

E

H

F

N

H

V

P

V

M

E

E

G

A

V

V

R

K

N
x
T

N

N

V

V

F

L

G

G

T

T

L

E

Y

N

T

V

A

L

Q

E

A

A

I

I

A

Q

T

N

G

H

V

V

E

K

T

R

F

V

V

V

L

C

I
x
L

S

S

T

T

D

D

K

K

A

A

V

V

R

Y

P

P

T

I

N

N

V

A

M

M

G

G

T

I

T

S

K
|
K

R

A

M

M

A

M

E

E

L

K

V

V

L

M

Q

V

A

A

L

K

S

S

K

R

L

N

N

L

S

E

G

G

-

L

-

D

T

T

R

V

F

I

C

C

M

G

V

T

R

R

F
x
Y

G

G

N

N

V

V

L

M

G

A

S

S

S

R

G

G

S

S

V

V

V

I

P

P

L

L

F

F

R

V

K

D

Q

Q

I

I

A

R

N

Q

G

G

G

K

P

P

V

L

T

T

V

I

T

T

H

D

P

P

E

N

I

M

T

T

R

R

F

F

F

M

M

M

T

T

I

L

P

E

E

D

A

A

S

V

Q

D

L

L

V

V

I

L

Q

Y

A

A

G

F

A

E

M

H

G

G

I

E

G

N

G

G

D

D

V

I

F

F

V

V

L

-

D

Q

M

K

G

A

Q

P

S

A

V

A

K

T

I

I

V

A

D

V

L

L

A

A

R

E

K

A

M

L

I

K

R

Q

L

L

S

L

G

N

F

V

E

E

V

-

K

-

D

-

E

-

H

-

N

-

P

-

D

-

G

-

D

D

I

H

E

P

I

I

Q

S

F

I

S

M

G

G

L

T

R

R

P

H

G

G

E

E

K

K

L

A

Y

F

E

E

E

A

L

L

I

S

-

R

-

E

-

M

-

V

-

H

-

A

-

F

-

D

-

Q

G

G

D

D

N

Y

V

F

E

R

G

V

T

P

G

A

H

D

E

E

R

D

I

Y

M

N

T

S

A

H

H

N

E

T

V

T

F

R

L

L

T

D

W

V

E

E

E

G

L

M

K

K

P

Q

V

L

L

L

D

L

K

K

L

L

D

D

binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G287), T13 (≠ G289), G14 (= G290), S15 (= S291), F16 (≠ I292), S36 (≠ E312), R37 (≠ L313), D38 (≠ S314), K41 (≠ G317), D60 (≠ S344), V61 (= V345), A80 (= A366), A81 (= A367), A82 (= A368), K84 (= K370), T99 (≠ N385), L122 (≠ I408), K138 (= K424), Y164 (≠ F448)

4g5hA Crystal structure of capsular polysaccharide synthesizing enzyme cape from staphylococcus aureus in complex with by-product (see paper)
33% identity, 50% coverage: 269:589/644 of query aligns to 4:333/346 of 4g5hA

query
sites
4g5hA

P

P

R

R

K

G

D

S

L

A

M

M

A

G

A

-

N

-

I

F

T

D

G

D

K

K

V

I

V

L

M

L

V

I

T

T

G
|
G

A

G

G
x
T

G
|
G

S
|
S

I
x
F

G

G

S

N

E

A

L

V

C

M

R

K

Q

R

I

F

L

L

R

D

L

S

A

N

P

I

R

K

K

E

L

I

V

R

L

I

F

F

E
x
S

L
x
R

S
x
D

E

E

F

-

G

-

L

-

Y

-

S

-

I

-

E

-

R

K

E
x
K

L

Q

Q

D

A

D

I

I

R

R

A

K

E

K

L

Y

G

N

L

-

D

N

V

S

E

K

L

L

F

K

P

F

M

Y

M

I

G

G

S
x
D

V
|
V

Q

R

R

D

Q

S

N

Q

R

S

V

V

Q

E

A

T

V

A

M

M

E

R

A

-

F

-

K

D

V

V

Q

D

T

Y

V

V

Y

F

H

H

A
|
A

Y

L

K
|
K

H
x
Q

V
|
V

P

P

L

S

V

C

E

E

H

F

N

F

V

P

V

V

E

E

G

A

V

V

R

K

N

T

N

N

V

I

F

I

G

G

T

T

L

E

Y

N

T

V

A

L

Q

Q

A

S

A

A

I

I

A

H

T

Q

G

N

V

V

E

K

T

K

F

V

V

I

L

C

I
x
L

S

S

T
|
T

D

D

K
|
K

A

A

V

A

R

Y

P

P

T

I

N

N

V

A

M

M

G

G

T

I

T

S

K
|
K

R

A

M

M

A

M

E

E

L

K

V

V

L

F

Q

V

A

A

L

K

S

S

K

R

-

N

-

I

L

R

N

S

S

E

G

Q

T

T

R

L

F

I

C

C

M

G

V

T

R

R

F
x
Y

G

G

N
|
N

V

V

L

M

G

A

S

S

S

R

G

G

S
|
S

V
|
V

V

I

P

P

L
|
L

F

F

R

I

K

D

Q

K

I

I

A

K

N

A

G

G

G

E

P

P

V

L

T
|
T

V

I

T
|
T

H

D

P

P

E

D

I
x
M

T

T

R

R

F

F

F

L

M

M

T

S

I

L

P

E

E

D

A

A

S

V

Q

E

L

L

V

V

I

V

Q

H

A

A

G

F

A

K

M

H

G

A

I

E

G

T

G

G

D

D

V

I

F

M

V

V

L

-

D

Q

M

K

G

A

Q

P

S

S

V

S

K

T

I
x
V

V

G

D

D

L

L

A

A

R

T

K

A

M

L

I

L

R

E

L

L

S

-

G

-

F

F

E

E

V

-

K

-

D

-

E

-

H

-

N

-

P

-

D

-

G

A

D

D

I

N

E

A

I

I

Q

E

F

I

S

I

G

G

L

T

R
|
R

P

H

G

G

E
|
E

K

K

L

K

Y

A

E

E

E

T

L

L

-

T

-

R

-

E

-
x
Y

-

A

-

Q

-

C

-

E

-

D

I

M

G

G

D

D

-

Y

-

F

-

R

-

V

-

P

-

A

-

D

-

S

-

R

-

D

-

L

-

N

-

Y

-

S

N

N

V

Y

E

V

G

E

T

T

G

G

H

N

E

E

R

K

I

I

M

T

T

Q

A
x
S

H
x
Y

E
|
E

V
x
Y

-

N

-

S

-

D

-
x
N

-

T

-

H

F

I

L

L

T

T

W

V

E

E

L

I

K

K

P

E

V

K

L

L

binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G287), T22 (≠ G289), G23 (= G290), S24 (= S291), F25 (≠ I292), S45 (≠ E312), R46 (≠ L313), D47 (≠ S314), K50 (≠ E324), D69 (≠ S344), V70 (= V345), A89 (= A366), A90 (= A367), A91 (= A368), K93 (= K370), L131 (≠ I408), T133 (= T410), K147 (= K424), Y173 (≠ F448)
binding [(2R,3R,4R,6R)-3-acetamido-6-methyl-4-oxidanyl-5-oxidanylidene-oxan-2-yl] [[(2R,3S,4R,5R)-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: Q94 (≠ H371), V95 (= V372), K135 (= K412), N175 (= N450), S182 (= S457), V183 (= V458), L186 (= L461), T198 (= T473), T200 (= T475), M204 (≠ I479), V240 (≠ I516), R263 (= R549), E266 (= E552), Y278 (vs. gap), S313 (≠ A575), Y314 (≠ H576), E315 (= E577), Y316 (≠ V578), N320 (vs. gap)

3w1vA Crystal structure of capsular polysaccharide synthesizing enzyme cape from staphylococcus aureus in complex with inihibitor (see paper)
33% identity, 50% coverage: 269:589/644 of query aligns to 4:333/347 of 3w1vA

query
sites
3w1vA

P

P

R

R

K

G

D

S

L

A

M

M

A

G

A

-

N

-

I

F

T

D

G

D

K

K

V

I

V

L

M

L

V

I

T

T

G

G

A

G

G

T

G

G

S

S

I

F

G

G

S

N

E

A

L

V

C

M

R

K

Q

R

I

F

L

L

R

D

L

S

A

N

P

I

R

K

K

E

L

I

V

R

L

I

F

F

E

S

L

R

S

D

E

E

F

-

G

-

L

-

Y

-

S

-

I

-

E

-

R

K

E

K

L

Q

Q

D

A

D

I

I

R

R

A

K

E

K

L

Y

G

N

L

-

D

N

V

S

E

K

L

L

F

K

P

F

M

Y

M

I

G

G

S

D

V

V

Q

R

R

D

Q

S

N

Q

R

S

V

V

Q

E

A

T

V

A

M

M

E

R

A

-

F

-

K

D

V

V

Q

D

T

Y

V

V

Y

F

H

H

A

A

Y

L

K
|
K

H
x
Q

V

V

P

P

L

S

V

C

E

E

H

F

N

F

V

P

V

V

E

E

G

A

V

V

R

K

N

T

N

N

V

I

F

I

G

G

T

T

L

E

Y

N

T

V

A

L

Q

Q

A

S

A

A

I

I

A

H

T

Q

G

N

V

V

E

K

T

K

F

V

V

I

L

C

I

L

S

S

T

T

D

D

K

K

A

A

V

A

R

Y

P

P

T

I

N

N

V

A

M

M

G

G

T

I

T

S

K

K

R

A

M

M

A

M

E

E

L

K

V

V

L

F

Q

V

A

A

L

K

S

S

K

R

-

N

-

I

L

R

N

S

S

E

G

Q

T

T

R

L

F

I

C

C

M

G

V

T

R

R

F

Y

G

G

N
|
N

V

V

L

M

G

A

S
|
S

S
x
R

G

G

S
|
S

V
|
V

V

I

P

P

L
|
L

F

F

R

I

K

D

Q

K

I

I

A

K

N

A

G

G

G

E

P

P

V

L

T
|
T

V
x
I

T
|
T

H

D

P

P

E

D

I
x
M

T

T

R
|
R

F

F

F

L

M

M

T

S

I

L

P

E

E

D

A

A

S

V

Q

E

L

L

V

V

I

V

Q

H

A

A

G

F

A

K

M

H

G

A

I

E

G

T

G

G

D

D

V

I

F

M

V

V

L

-

D

Q

M

K

G

A

Q

P

S

S

V

S

K

T

I
x
V

V

G

D

D

L

L

A

A

R

T

K

A

M

L

I

L

R

E

L

L

S

-

G

-

F

F

E

E

V

-

K

-

D

-

E

-

H

-

N

-

P

-

D

-

G

A

D

D

I

N

E

A

I

I

Q

E

F

I

S

I

G

G

L

T

R
|
R

P

H

G

G

E
|
E

K

K

L

K

Y

A

E

E

E

T

L

L

-

T

-

R

-

E

-

Y

-

A

-

Q

-

C

-

E

-

D

I

M

G

G

D

D

-

Y

-

F

-

R

-

V

-

P

-

A

-

D

-

S

-

R

-

D

-

L

-

N

-

Y

-

S

N

N

V

Y

E

V

G

E

T

T

G

G

H

N

E

E

R

K

I

I

M

T

T

Q

A

S

H

Y

E

E

V

Y

-

N

-

S

-

D

-

N

-

T

-

H

F

I

L

L

T

T

W

V

E

E

L

I

K

K

P

E

V

K

L

L

binding [(2R,3S,4R,5R,6R)-5-acetamido-6-[[[(2R,3S,4R,5R)-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,4-bis(oxidanyl)oxan-2-yl]methylimino-azanylidene-azanium: K93 (= K370), Q94 (≠ H371), N175 (= N450), S179 (= S454), R180 (≠ S455), S182 (= S457), V183 (= V458), L186 (= L461), T198 (= T473), I199 (≠ V474), T200 (= T475), M204 (≠ I479), R206 (= R481), V240 (≠ I516), R263 (= R549), E266 (= E552)

3vvcA Crystal structure of capsular polysaccharide synthesizing enzyme cape , k126e, in apo form (see paper)
35% identity, 43% coverage: 281:559/644 of query aligns to 6:265/318 of 3vvcA

query
sites
3vvcA

K

K

V

I

V

L

M

L

V

I

T

T

G
|
G

A

G

G
x
T

G
|
G

S

S

I
x
F

G

G

S

N

E

A

L

V

C

M

R

K

Q

R

I

F

L

L

R

D

L

S

A

N

P

I

R

K

K

E

L

I

V

R

L

I

F

F

E
x
S

L
x
R

S
x
D

E

E

F

-

G

-

L

-

Y

-

S

-

I

-

E

-

R

K

E
x
K

L

Q

Q

D

A

D

I

I

R

R

A

K

E

K

L

Y

G

N

L

-

D

N

V

S

E

K

L

L

F

K

P

F

M

Y

M

I

G

G

S
x
D

V
|
V

Q
x
R

R

D

Q

S

N

Q

R

S

V

V

Q

E

A

T

V

A

M

M

E

R

A

-

F

-

K

D

V

V

Q

D

T

Y

V

V

Y

F

H

H

A
|
A

Y

L

K
|
K

H

Q

V

V

P

P

L

S

V

C

E

E

H

F

N

F

V

P

V

V

E

E

G

A

V

V

R

K

N

T

N

N

V

I

F

I

G

G

T

T

L

E

Y

N

T

V

A

L

Q

Q

A

S

A

A

I

I

A

H

T

Q

G

N

V

V

E

K

T

K

F

V

V

I

L

C

I

L

S
|
S

T
|
T

D

D

K

E

A

A

V

A

R

Y

P

P

T

I

N

N

V

A

M

M

G

G

T

I

T

S

K
|
K

R

A

M

M

A

M

E

E

L

K

V

V

L

F

Q

V

A

A

L

K

S

S

K

R

-

N

-

I

L

R

N

S

S

E

G

Q

T

T

R

L

F

I

C

C

M

G

V

T

R

R

F
x
Y

G
|
G

N

N

V

V

L

M

G

A

S

S

S

R

G

G

S

S

V

V

V

I

P

P

L

L

F

F

R

I

K

D

Q

K

I

I

A

K

N

A

G

G

G

E

P

P

V

L

T

T

V

I

T

T

H

D

P

P

E

D

I

M

T

T

R

R

F

F

F

L

M

M

T

S

I

L

P

E

E

D

A

A

S

V

Q

E

L

L

V

V

I

V

Q

H

A

A

G

F

A

K

M

H

G

A

I

E

G

T

G

G

D

D

V

I

F

M

V

V

L

-

D

Q

M

K

G

A

Q

P

S

S

V

S

K

T

I

V

V

G

D

D

L

L

A

A

R

T

K

A

M

L

I

L

R

E

L

L

S

-

G

-

F

F

E

E

V

-

K

-

D

-

E

-

H

-

N

-

P

-

D

-

G

A

D

D

I

N

E

A

I

I

Q

E

F

I

S

I

G

G

L

T

R

R

P

H

G

G

E

E

K

K

L

K

Y

A

E

E

E

T

L

L

binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G287), T14 (≠ G289), G15 (= G290), F17 (≠ I292), S37 (≠ E312), R38 (≠ L313), D39 (≠ S314), K42 (≠ E324), D61 (≠ S344), V62 (= V345), R63 (≠ Q346), A81 (= A366), A82 (= A367), A83 (= A368), K85 (= K370), S124 (= S409), T125 (= T410), K139 (= K424), Y165 (≠ F448), G166 (= G449)

2gn8A Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADP and udp (see paper)
30% identity, 44% coverage: 278:562/644 of query aligns to 2:265/327 of 2gn8A

query
sites
2gn8A

I

L

T

D

G

N

K

Q

V

T

V

I

M

L

V

I

T

T

G
|
G

A

G

G
x
T

G
|
G

S
|
S

I
x
F

G

G

S

K

E

C

L

F

C

V

R

R

Q

K

I

V

L

L

R

D

L

T

A

T

-

N

P

A

R

K

K

K

L

I

V

I

L

V

F

Y

E
x
S

L
x
R

S
x
D

E

E

F

L

G
x
K

L

Q

Y

S

S

E

I

M

E

A

R

M

E

E

L

F

Q

N

A

D

I

P

R

R

A

M

E

R

L

F

G

-

L

-

D

-

V

-

E

-

L

-

F

-

P

-

M

F

M

I

G

G

S
x
D

V
|
V

Q

R

R

D

Q

L

N

E

R

R

V

L

Q

N

A

Y

V

A

M

L

E

E

A

G

F

-

K

-

V

V

Q

D

T

I

V

C

Y

I

H

H

A
|
A

Y

L

K
|
K

H

H

V

V

P

P

L

I

V

A

E

E

H

Y

N

N

V

P

V

L

E

E

G

C

V

I

R

K

N
x
T

N

N

V

I

F

M

G

G

T

A

L

S

Y

N

T

V

A

I

Q

N

A

A

A

C

I

L

A

K

T

N

G

A

V

I

E

S

T

Q

F

V

V

I

L

A

I
x
L

S
|
S

T

T

D

D

K
|
K

A

A

V

A

R

N

P

P

T

I

N

N

V

L

M

Y

G

G

T

A

T

T

K
|
K

R

L

M

C

A

S

E

D

L

K

V

L

L

F

Q

V

A

S

L

A

S

N

-

N

-

F

K

K

L

G

N

S

S

S

G

Q

T

T

R

Q

F

F

C

S

M

V

V

V

R

R

F
x
Y

G
|
G

N
|
N

V
|
V

L

V

G

G

S
|
S

S
x
R

G

G

S

S

V
|
V

V

V

P

P

L

F

F

F

R

K

K

K

Q

L

I

V

A

Q

N

N

-

K

G

A

G

S

P

E

V

I

T
x
P

V
x
I

T
|
T

H

D

P

I

E

R

I
x
M

T

T

R
|
R

F

F

F

W

M

I

T

T

I

L

P

D

E

E

A

G

S

V

Q

S

L

F

V

V

I

L

Q

K

A

S

G

L

A

K

M

R

G

M

I

H

G

G

D

E

V

I

F

F

V

V

L

P

D

K

M

I

G

-

Q

P

S

S

V

M

K

K

I
x
M

V

T

D

D

L

L

A

A

R

K

K

A

M

L

I

A

R

-

L

-

S

-

G

-

F

-

E

-

V

-

K

-

D

-

E

-

H

-

N

-

P

P

D

N

G

T

D

P

I

T

E

K

I

I

Q

-

F

-

S

I

G

G

L

I

R
|
R

P

P

G

G

E

E

K

K

L

L

Y

H

E

E

E

V

L

M

L

I

I

P

G

K

D

D

binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G287), T13 (≠ G289), G14 (= G290), S15 (= S291), F16 (≠ I292), S37 (≠ E312), R38 (≠ L313), D39 (≠ S314), K42 (≠ G317), D61 (≠ S344), V62 (= V345), A81 (= A366), A82 (= A367), A83 (= A368), K85 (= K370), T100 (≠ N385), L123 (≠ I408), S124 (= S409), K139 (= K424), Y165 (≠ F448), G166 (= G449), V168 (= V451), S171 (= S454), R172 (≠ S455)
binding uridine-5'-diphosphate: K127 (= K412), N167 (= N450), V175 (= V458), P191 (≠ T473), I192 (≠ V474), T193 (= T475), M197 (≠ I479), R199 (= R481), M233 (≠ I516), R252 (= R549)

O25511 UDP-N-acetylglucosamine 4,6-dehydratase (inverting); Pseudaminic acid biosynthesis protein B; UDP-GlcNAc-inverting 4,6-dehydratase; EC 4.2.1.115 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see paper)
30% identity, 44% coverage: 278:562/644 of query aligns to 8:271/333 of O25511

query
sites
O25511

I

L

T

D

G

N

K

Q

V

T

V

I

M

L

V

I

T

T

G

G

A

G

G
x
T

G
|
G

S
|
S

I
x
F

G

G

S

K

E

C

L

F

C

V

R

R

Q

K

I

V

L

L

R

D

L

T

A

T

-

N

P

A

R

K

K

K

L

I

V

I

L

V

F

Y

E
x
S

L
x
R

S
x
D

E
|
E

F
x
L

G
x
K

L

Q

Y

S

S

E

I

M

E

A

R

M

E

E

L

F

Q

N

A

D

I

P

R

R

A

M

E

R

L

F

G

-

L

-

D

-

V

-

E

-

L

-

F

-

P

-

M

F

M

I

G

G

S
x
D

V
|
V

Q

R

R

D

Q

L

N

E

R

R

V

L

Q

N

A

Y

V

A

M

L

E

E

A

G

F

-

K

-

V

V

Q

D

T

I

V

C

Y

I

H

H

A
|
A

A

A

Y

L

K
|
K

H

H

V

V

P

P

L

I

V

A

E

E

H

Y

N

N

V

P

V

L

E

E

G

C

V

I

R

K

N

T

N

N

V

I

F

M

G

G

T

A

L

S

Y

N

T

V

A

I

Q

N

A

A

A

C

I

L

A

K

T

N

G

A

V

I

E

S

T

Q

F

V

V

I

L

A

I
x
L

S
|
S

T

T

D

D

K
|
K

A

A

V

A

R

N

P

P

T

I

N

N

V

L

M
x
Y

G

G

T

A

T

T

K
|
K

R

L

M

C

A

S

E

D

L

K

V

L

L

F

Q

V

A

S

L

A

S

N

-

N

-

F

K

K

L

G

N

S

S

S

G

Q

T

T

R

Q

F

F

C

S

M

V

V

V

R

R

F

Y

G

G

N

N

V
|
V

L
x
V

G
|
G

S
|
S

S
x
R

G

G

S

S

V

V

P

P

L

F

F

F

R

K

K

K

Q

L

I

V

A

Q

N

N

-

K

G

A

G

S

P

E

V

I

T

P

V

I

T

T

H

D

P

I

E

R

I

M

T

T

R

R

F

F

F

W

M

I

T

T

I

L

P

D

E

E

A

G

S

V

Q

S

L

F

V

V

I

L

Q

K

A

S

G

L

A

K

M

R

G

M

I

H

G

G

D

E

V

I

F

F

V

V

L

P

D

K

M

I

G

-

Q

P

S

S

V

M

K

K

I

M

V

T

D

D

L

L

A

A

R

K

K

A

M

L

I

A

R

-

L

-

S

-

G

-

F

-

E

-

V

-

K

-

D

-

E

-

H

-

N

-

P

P

D

N

G

T

D

P

I

T

E

K

I

I

Q

-

F

-

S

I

G

G

L

I

R

R

P

P

G

G

E

E

K

K

L

L

Y

H

E

E

E

V

L

M

L

I

I

P

G

K

D

D

TGSF 19:22 (≠ GGSI 289:292) binding
SRDELK 43:48 (≠ ELSEFG 312:317) binding
DV 67:68 (≠ SV 344:345) binding
A87 (= A366) binding
K91 (= K370) binding
LS 129:130 (≠ IS 408:409) binding
K133 (= K412) mutation K->A,E: Loss of activity.
Y141 (≠ M420) binding
K145 (= K424) binding
VVGSR 174:178 (≠ VLGSS 451:455) binding

2gnaA Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADP and udp-gal (see paper)
30% identity, 44% coverage: 278:562/644 of query aligns to 4:267/329 of 2gnaA

query
sites
2gnaA

I

L

T

D

G

N

K

Q

V

T

V

I

M

L

V

I

T

T

G
|
G

A

G

G
x
T

G
|
G

S
|
S

I
x
F

G

G

S

K

E

C

L

F

C

V

R

R

Q

K

I

V

L

L

R

D

L

T

A

T

-

N

P

A

R

K

K

K

L

I

V

I

L

V

F

Y

E
x
S

L
x
R

S
x
D

E

E

F

L

G
x
K

L

Q

Y

S

S

E

I

M

E

A

R

M

E

E

L

F

Q

N

A

D

I

P

R

R

A

M

E

R

L

F

G

-

L

-

D

-

V

-

E

-

L

-

F

-

P

-

M

F

M

I

G

G

S
x
D

V
|
V

Q

R

R

D

Q

L

N

E

R

R

V

L

Q

N

A

Y

V

A

M

L

E

E

A

G

F

-

K

-

V

V

Q

D

T

I

V

C

Y

I

H

H

A
|
A

Y

L

K
|
K

H

H

V

V

P

P

L

I

V

A

E

E

H

Y

N

N

V

P

V

L

E

E

G

C

V

I

R

K

N

T

N

N

V

I

F

M

G

G

T

A

L

S

Y

N

T

V

A

I

Q

N

A

A

A

C

I

L

A

K

T

N

G

A

V

I

E

S

T

Q

F

V

V

I

L

A

I
x
L

S
|
S

T

T

D

D

K

K

A

A

V

A

R

N

P

P

T

I

N

N

V

L

M
x
Y

G

G

T

A

T

T

K
|
K

R

L

M

C

A

S

E

D

L

K

V

L

L

F

Q

V

A

S

L

A

S

N

-

N

-

F

K

K

L

G

N

S

S

S

G

Q

T

T

R

Q

F

F

C

S

M

V

V

V

R

R

F
x
Y

G
|
G

N

N

V
|
V

L

V

G

G

S

S

S

R

G

G

S
|
S

V
|
V

V

V

P

P

L

F

F

F

R

K

K

K

Q

L

I

V

A

Q

N

N

-

K

G

A

G

S

P

E

V

I

T

P

V

I

T
|
T

H

D

P

I

E

R

I
x
M

T

T

R
|
R

F

F

F

W

M

I

T

T

I

L

P

D

E

E

A

G

S

V

Q

S

L

F

V

V

I

L

Q

K

A

S

G

L

A

K

M

R

G

M

I

H

G

G

D

E

V

I

F

F

V

V

L

P

D

K

M

I

G

-

Q

P

S

S

V

M

K

K

I
x
M

V

T

D

D

L

L

A

A

R

K

K

A

M

L

I

A

R

-

L

-

S

-

G

-

F

-

E

-

V

-

K

-

D

-

E

-

H

-

N

-

P

P

D

N

G

T

D

P

I

T

E

K

I

I

Q

-

F

-

S

I

G

G

L

I

R
|
R

P

P

G

G

E
|
E

K

K

L

L

Y

H

E

E

E

V

L

M

L

I

I

P

G

K

D

D

binding galactose-uridine-5'-diphosphate: K87 (= K370), S176 (= S457), V177 (= V458), T195 (= T475), M199 (≠ I479), R201 (= R481), M235 (≠ I516), R254 (= R549), E257 (= E552)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G287), T15 (≠ G289), G16 (= G290), S17 (= S291), F18 (≠ I292), S39 (≠ E312), R40 (≠ L313), D41 (≠ S314), K44 (≠ G317), D63 (≠ S344), V64 (= V345), A83 (= A366), A84 (= A367), A85 (= A368), K87 (= K370), L125 (≠ I408), S126 (= S409), Y137 (≠ M420), K141 (= K424), Y167 (≠ F448), G168 (= G449), V170 (= V451)

2gn9A Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADP and udp-glc (see paper)
30% identity, 44% coverage: 278:562/644 of query aligns to 4:267/329 of 2gn9A

query
sites
2gn9A

I

L

T

D

G

N

K

Q

V

T

V

I

M

L

V

I

T

T

G
|
G

A

G

G
x
T

G
|
G

S
|
S

I
x
F

G

G

S

K

E

C

L

F

C

V

R

R

Q

K

I

V

L

L

R

D

L

T

A

T

-

N

P

A

R

K

K

K

L

I

V

I

L

V

F

Y

E

S

L
x
R

S
x
D

E

E

F

L

G
x
K

L

Q

Y

S

S

E

I

M

E

A

R

M

E

E

L

F

Q

N

A

D

I

P

R

R

A

M

E

R

L

F

G

-

L

-

D

-

V

-

E

-

L

-

F

-

P

-

M

F

M

I

G

G

S
x
D

V
|
V

Q

R

R

D

Q

L

N

E

R

R

V

L

Q

N

A

Y

V

A

M

L

E

E

A

G

F

-

K

-

V

V

Q

D

T

I

V

C

Y

I

H

H

A
|
A

Y

L

K
|
K

H

H

V

V

P

P

L

I

V

A

E

E

H

Y

N

N

V

P

V

L

E

E

G

C

V

I

R

K

N

T

N

N

V

I

F

M

G

G

T

A

L

S

Y

N

T

V

A

I

Q

N

A

A

A

C

I

L

A

K

T

N

G

A

V

I

E

S

T

Q

F

V

V

I

L

A

I
x
L

S
|
S

T
|
T

D

D

K
|
K

A

A

V

A

R

N

P

P

T

I

N

N

V

L

M
x
Y

G

G

T

A

T

T

K
|
K

R

L

M

C

A

S

E

D

L

K

V

L

L

F

Q

V

A

S

L

A

S

N

-

N

-

F

K

K

L

G

N

S

S

S

G

Q

T

T

R

Q

F

F

C

S

M

V

V

V

R

R

F
x
Y

G
|
G

N
|
N

V
|
V

L

V

G

G

S

S

S
x
R

G

G

S
|
S

V
|
V

V

V

P

P

L

F

F

F

R

K

K

K

Q

L

I

V

A

Q

N

N

-

K

G

A

G

S

P

E

V

I

T
x
P

V

I

T
|
T

H

D

P

I

E

R

I
x
M

T

T

R
|
R

F

F

F

W

M

I

T

T

I

L

P

D

E

E

A

G

S

V

Q

S

L

F

V

V

I

L

Q

K

A

S

G

L

A

K

M

R

G

M

I

H

G

G

D

E

V

I

F

F

V

V

L

P

D

K

M

I

G

-

Q

P

S

S

V

M

K

K

I
x
M

V

T

D

D

L

L

A

A

R

K

K

A

M

L

I

A

R

-

L

-

S

-

G

-

F

-

E

-

V

-

K

-

D

-

E

-

H

-

N

-

P

P

D

N

G

T

D

P

I

T

E

K

I

I

Q

-

F

-

S

I

G

G

L

I

R
|
R

P

P

G

G

E
|
E

K

K

L

L

Y

H

E

E

E

V

L

M

L

I

I

P

G

K

D

D

binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G287), T15 (≠ G289), G16 (= G290), S17 (= S291), F18 (≠ I292), R40 (≠ L313), D41 (≠ S314), K44 (≠ G317), D63 (≠ S344), V64 (= V345), A83 (= A366), A84 (= A367), A85 (= A368), K87 (= K370), L125 (≠ I408), S126 (= S409), K141 (= K424), Y167 (≠ F448), G168 (= G449), V170 (= V451), R174 (≠ S455)
binding uridine-5'-diphosphate-glucose: K87 (= K370), T127 (= T410), K129 (= K412), Y137 (≠ M420), N169 (= N450), S176 (= S457), V177 (= V458), P193 (≠ T473), T195 (= T475), M199 (≠ I479), R201 (= R481), M235 (≠ I516), R254 (= R549), E257 (= E552)

2gn6A Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADP and udp-glcnac (see paper)
30% identity, 44% coverage: 278:562/644 of query aligns to 4:267/329 of 2gn6A

query
sites
2gn6A

I

L

T

D

G

N

K

Q

V

T

V

I

M

L

V

I

T

T

G
|
G

A

G

G
x
T

G
|
G

S
|
S

I
x
F

G

G

S

K

E

C

L

F

C

V

R

R

Q

K

I

V

L

L

R

D

L

T

A

T

-

N

P

A

R

K

K

K

L

I

V

I

L

V

F

Y

E

S

L
x
R

S
x
D

E

E

F

L

G
x
K

L

Q

Y

S

S

E

I

M

E

A

R

M

E

E

L

F

Q

N

A

D

I

P

R

R

A

M

E

R

L

F

G

-

L

-

D

-

V

-

E

-

L

-

F

-

P

-

M

F

M

I

G

G

S
x
D

V
|
V

Q

R

R

D

Q

L

N

E

R

R

V

L

Q

N

A

Y

V

A

M

L

E

E

A

G

F

-

K

-

V

V

Q

D

T

I

V

C

Y

I

H

H

A

A

A
|
A

Y

L

K
|
K

H

H

V

V

P

P

L

I

V

A

E

E

H

Y

N

N

V

P

V

L

E

E

G

C

V

I

R

K

N

T

N

N

V

I

F

M

G

G

T

A

L

S

Y

N

T

V

A

I

Q

N

A

A

A

C

I

L

A

K

T

N

G

A

V

I

E

S

T

Q

F

V

V

I

L

A

I

L

S
|
S

T

T

D
|
D

K
|
K

A

A

V

A

R

N

P

P

T

I

N

N

V

L

M
x
Y

G

G

T

A

T

T

K
|
K

R

L

M

C

A

S

E

D

L

K

V

L

L

F

Q

V

A

S

L

A

S

N

-

N

-

F

K

K

L

G

N

S

S

S

G

Q

T

T

R

Q

F

F

C

S

M

V

V

V

R

R

F
x
Y

G
|
G

N
|
N

V
|
V

L

V

G

G

S
|
S

S
x
R

G
|
G

S
|
S

V
|
V

V

V

P

P

L

F

F

F

R

K

K

K

Q

L

I

V

A

Q

N

N

-

K

G

A

G

S

P

E

V

I

T
x
P

V
x
I

T

T

H

D

P

I

E

R

I
x
M

T

T

R
|
R

F

F

F

W

M

I

T

T

I

L

P

D

E

E

A

G

S

V

Q

S

L

F

V

V

I

L

Q

K

A

S

G

L

A

K

M

R

G

M

I

H

G

G

D

E

V

I

F

F

V

V

L

P

D

K

M

I

G

-

Q

P

S

S

V

M

K

K

I
x
M

V

T

D

D

L

L

A

A

R

K

K

A

M

L

I

A

R

-

L

-

S

-

G

-

F

-

E

-

V

-

K

-

D

-

E

-

H

-

N

-

P

P

D

N

G

T

D

P

I

T

E

K

I

I

Q

-

F

-

S

I

G

G

L

I

R
|
R

P

P

G

G

E
|
E

K

K

L

L

Y

H

E

E

E

V

L

M

L

I

I

P

G

K

D

D

binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G287), T15 (≠ G289), G16 (= G290), S17 (= S291), F18 (≠ I292), R40 (≠ L313), D41 (≠ S314), K44 (≠ G317), D63 (≠ S344), V64 (= V345), A84 (= A367), A85 (= A368), K87 (= K370), S126 (= S409), Y137 (≠ M420), K141 (= K424), Y167 (≠ F448), G168 (= G449), V170 (= V451), S173 (= S454), R174 (≠ S455)
binding uridine-diphosphate-n-acetylglucosamine: K87 (= K370), D128 (= D411), K129 (= K412), N169 (= N450), G175 (= G456), S176 (= S457), V177 (= V458), P193 (≠ T473), I194 (≠ V474), M199 (≠ I479), R201 (= R481), M235 (≠ I516), R254 (= R549), E257 (= E552)

2gn4A Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADPH and udp-glcnac (see paper)
30% identity, 44% coverage: 278:562/644 of query aligns to 4:267/329 of 2gn4A

query
sites
2gn4A

I

L

T

D

G

N

K

Q

V

T

V

I

M

L

V

I

T

T

G
|
G

A

G

G
x
T

G
|
G

S
|
S

I
x
F

G

G

S

K

E

C

L

F

C

V

R

R

Q

K

I

V

L

L

R

D

L

T

A

T

-

N

P

A

R

K

K

K

L

I

V

I

L

V

F

Y

E
x
S

L
x
R

S
x
D

E

E

F

L

G
x
K

L

Q

Y

S

S

E

I

M

E

A

R

M

E

E

L

F

Q

N

A

D

I

P

R

R

A

M

E

R

L

F

G

-

L

-

D

-

V

-

E

-

L

-

F

-

P

-

M

F

M

I

G

G

S
x
D

V
|
V

Q

R

R

D

Q

L

N

E

R

R

V

L

Q

N

A

Y

V

A

M

L

E

E

A

G

F

-

K

-

V

V

Q

D

T

I

V

C

Y

I

H

H

A
|
A

Y

L

K
|
K

H

H

V

V

P

P

L

I

V

A

E

E

H

Y

N

N

V

P

V

L

E

E

G

C

V

I

R

K

N
x
T

N

N

V

I

F

M

G

G

T

A

L

S

Y

N

T

V

A

I

Q

N

A

A

A

C

I

L

A

K

T

N

G

A

V

I

E

S

T

Q

F

V

V

I

L

A

I
x
L

S
|
S

T
|
T

D
|
D

K
|
K

A

A

V

A

R

N

P

P

T

I

N

N

V

L

M
x
Y

G

G

T

A

T

T

K
|
K

R

L

M

C

A

S

E

D

L

K

V

L

L

F

Q

V

A

S

L

A

S

N

-

N

-

F

K

K

L

G

N

S

S

S

G

Q

T

T

R

Q

F

F

C

S

M

V

V

V

R

R

F
x
Y

G
|
G

N
|
N

V
|
V

L

V

G

G

S
|
S

S
x
R

G

G

S
|
S

V
|
V

V

V

P

P

L

F

F

F

R

K

K

K

Q

L

I

V

A

Q

N

N

-

K

G

A

G

S

P

E

V

I

T
x
P

V

I

T
|
T

H

D

P

I

E

R

I
x
M

T

T

R
|
R

F

F

F

W

M

I

T

T

I

L

P

D

E

E

A

G

S

V

Q

S

L

F

V

V

I

L

Q

K

A

S

G

L

A

K

M

R

G

M

I

H

G

G

D

E

V

I

F

F

V

V

L

P

D

K

M

I

G

-

Q

P

S

S

V

M

K

K

I
x
M

V

T

D

D

L

L

A

A

R

K

K

A

M

L

I

A

R

-

L

-

S

-

G

-

F

-

E

-

V

-

K

-

D

-

E

-

H

-

N

-

P

P

D

N

G

T

D

P

I

T

E

K

I

I

Q

-

F

-

S

I

G

G

L

I

R
|
R

P

P

G

G

E
|
E

K

K

L

L

Y

H

E

E

E

V

L

M

L

I

I

P

G

K

D

D

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G13 (= G287), T15 (≠ G289), G16 (= G290), S17 (= S291), F18 (≠ I292), S39 (≠ E312), R40 (≠ L313), D41 (≠ S314), K44 (≠ G317), D63 (≠ S344), V64 (= V345), A83 (= A366), A84 (= A367), A85 (= A368), K87 (= K370), T102 (≠ N385), L125 (≠ I408), S126 (= S409), T127 (= T410), Y137 (≠ M420), K141 (= K424), Y167 (≠ F448), G168 (= G449), V170 (= V451), S173 (= S454), R174 (≠ S455)
binding uridine-diphosphate-n-acetylglucosamine: K87 (= K370), T127 (= T410), D128 (= D411), K129 (= K412), Y137 (≠ M420), N169 (= N450), S176 (= S457), V177 (= V458), P193 (≠ T473), T195 (= T475), M199 (≠ I479), R201 (= R481), M235 (≠ I516), R254 (= R549), E257 (= E552)

3pvzA Udp-n-acetylglucosamine 4,6-dehydratase from vibrio fischeri
27% identity, 42% coverage: 261:530/644 of query aligns to 5:279/372 of 3pvzA

query
sites
3pvzA

L

L

L

I

G

G

R

R

D

D

A

T

V

E

A

L

P

F

R

H

K

Q

D

D

L

I

M

N

A

A

A

N

N

E

-

K

-

E

-

L

-

Q

-

S

-

V

I

V

T

S

G

Q

K

S

V

R

V

F

M

L

V

V

T

L

G

G

A

G

G

A

G
|
G

S
|
S

I
|
I

G

G

S

Q

E

A

L

V

C

T

R

K

Q

E

I

I

L

F

R

K

L

R

A

N

P

P

R

Q

K

K

L

L

V

H

L

V

F

V

E
x
D

L
x
I

S

S

E

E

F

N

G

N

L

M

Y

V

S

E

I

L

E

V

R

R

E

D

L

I

Q

R

A

S

I

S

R

F

A

G

E

Y

L

I

G

N

L

G

D

D

V

F

E

Q

L

T

F

F

P

A

M

L

-
x
D

M
x
I

G

G

S

S

V

I

Q

E

R

Y

Q

D

N

A

R

F

V

I

Q

K

A

A

V

-

M

-

E

-

A

D

F

G

K

Q

V

Y

Q

D

T

Y

V

V

Y

L

H

N

A
x
L

A
x
S

A
|
A

Y

L

K
|
K

H

H

V

V

P

R

L

S

V

E

E

K

H

D

-

P

-

F

N

T

V

L

V

M

E

R

G

M

V

I

R

D

N

V

N

N

V

V

F

F

G

N

T

T

L

D

Y

K

T

T

A

I

Q

Q

A

Q

A

S

I

I

A

D

T

A

G

G

V

A

E

K

T

K

F

Y

V

F

L

C

I
x
V

S

S

T

T

D
|
D

K

K

A

A

V

A

R

N

P

P

T

V

N

N

V

M

M

M

G

G

T

A

T

S

K
|
K

R

R

M

I

A

M

E

E

L

M

V

F

L

L

Q

M

A

R

L

K

S

S

K

E

L

-

N

-

S

-

G

E

T

I

R

A

F

I

C

S

M

T

V

A

R

R

F

F

G

A

N

N

V
|
V

L

A

G

F

S
|
S

S

D

G

G

S

S

V

L

P

H

L

G

F

F

R

N

K

Q

Q

R

I

I

A

Q

N

K

G

N

G

Q

P

P

V

I

T

-

V

V

T

A

H

P

P

N

E

D

I

I

T

K

R

R

F

Y

F

F

M

V

T

T

I

P

P

Q

E

E

A

S

S

G

Q

E

L

L

V

C

I

L

Q

M

A

S

G

C

A

I

M

F

G

G

I

E

G

N

G

R

D

D

V

I

F

F

V

F

L

P

D

K

M

L

G

S

Q

E

S

A

V

L

K

H

I

L

V

I

-

S

-

F

-

A

D

D

L

I

A

A

R

V

K

K

M

Y

I

L

R

K

L

Q

S

L

G

G

F

Y

E

E

binding nicotinamide-adenine-dinucleotide: G40 (= G290), S41 (= S291), I42 (= I292), D62 (≠ E312), I63 (≠ L313), D92 (vs. gap), I93 (≠ M342), L114 (≠ A366), S115 (≠ A367), A116 (= A368), K118 (= K370), V158 (≠ I408), D161 (= D411), K174 (= K424), V198 (= V451), S201 (= S454)

3vvbA Crystal structure of capsular polysaccharide synthesizing enzyme cape from staphylococcus aureus in apo form (see paper)
30% identity, 35% coverage: 281:507/644 of query aligns to 4:198/270 of 3vvbA

query
sites
3vvbA

K

K

V

I

V

L

M

L

V

I

T

T

G
|
G

A

G

G
x
T

G
|
G

S
|
S

I
x
F

G

G

S

N

E

A

L

V

C

M

R

K

Q

R

I

F

L

L

R

D

L

S

A

N

P

I

R

K

K

E

L

I

V

R

L

I

F

F

E
x
S

L
x
R

S
x
D

E

E

F

-

G

-

L

-

Y

-

S

-

I

-

E

-

R

K

E
x
K

L

Q

Q

D

A

D

I

I

R

R

A

K

E

K

L

Y

G

N

L

-

D

N

V

S

E

K

L

L

F

K

P

F

M

Y

M

I

G

G

S
x
D

V
|
V

Q

R

R

D

Q

S

N

Q

R

S

V

V

Q

E

A

T

V

A

M

M

E

R

A

-

F

-

K

D

V

V

Q

D

T

Y

V

V

Y

F

H

H

A

A

A
|
A

Y

L

K
|
K

H

Q

V

V

P

P

L

S

V

C

E

E

H

F

N

F

V

P

V

V

E

E

G

A

V

V

R

K

N

T

N

N

V

I

F

I

G

G

T

T

L

E

Y

N

T

V

A

L

Q

Q

A

S

A

A

I

I

A

H

T

Q

G

N

V

V

E

K

T

K

F

V

V

I

L

C

I
x
L

S

S

T
|
T

D

D

K

K

A

A

V

A

R

Y

P

P

T

I

N

N

V

A

M

M

G

G

T

I

T

S

K
|
K

R

A

M

M

A

M

E

E

L

K

V

V

L

F

Q

V

A

A

L

K

S

S

K

R

-

N

-

I

L

R

N

S

S

E

G

Q

T

T

R

L

F

I

C

C

M

G

V

T

R

R

F
x
Y

G
|
G

N

N

V

V

L

M

G

-

S

-

G

-

S

-

V

-

P

-

L

-

F

-

R

-

K

-

Q

-

I

-

A

-

N

-

G

-

P

-

V

-

T

-

V

-

T

-

H

-

P

P

E

D

I

M

T

T

R

R

F

F

F

L

M

M

T

S

I

L

P

E

E

D

A

A

S

V

Q

E

L

L

V

V

I

V

Q

H

A

A

G

F

A

K

M

H

G

A

I

E

G

T

G

G

D

D

V

I

F

M

V

V

binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G287), T12 (≠ G289), G13 (= G290), S14 (= S291), F15 (≠ I292), S35 (≠ E312), R36 (≠ L313), D37 (≠ S314), K40 (≠ E324), D59 (≠ S344), V60 (= V345), A80 (= A367), A81 (= A368), K83 (= K370), L121 (≠ I408), T123 (= T410), K137 (= K424), Y163 (≠ F448), G164 (= G449)

3pvzB Udp-n-acetylglucosamine 4,6-dehydratase from vibrio fischeri
27% identity, 38% coverage: 261:506/644 of query aligns to 3:236/300 of 3pvzB

query
sites
3pvzB

L

L

L

I

G

G

R

R

D

D

A

T

V

E

A

L

P

F

R

H

K

Q

D

D

L

I

M

N

A

A

A

N

N

E

-

K

-

E

-

L

-

Q

-

S

-

V

I

V

T

S

G

Q

K

S

V

R

V

F

M

L

V

V

T

L

G

G

A

G

G

A

G
|
G

S
|
S

I
|
I

G

G

S

Q

E

A

L

V

C

T

R

K

Q

E

I

I

L

F

R

K

L

R

A

N

P

P

R

Q

K

K

L

L

V

H

L

V

F

V

E
x
D

L
x
I

S

S

E

E

F

N

G

N

L

M

Y

V

S

E

I

L

E

V

R

R

E

D

L

I

Q

R

A

S

I

S

R

F

A

G

E

Y

L

I

G

N

L

G

D

D

V

F

E

Q

L

T

F

F

P

A

M
x
L

-
x
D

M
x
I

G

G

S

S

V

I

Q

E

R

Y

Q

D

N

A

R

F

V

I

Q

K

A

A

V

-

M

-

E

-

A

D

F

G

K

Q

V

Y

Q

D

T

Y

V

V

Y

L

H

N

A
x
L

A
x
S

A
|
A

Y

L

K
|
K

H

H

V

V

P

R

L

S

V

E

E

K

H

D

-

P

-

F

N

T

V

L

V

M

E

R

G

M

V

I

R

D

N

V

N

N

V

V

F

F

G

N

T

T

L

D

Y

K

T

T

A

I

Q

Q

A

Q

A

S

I

I

A

D

T

A

G

G

V

A

E

K

T

K

F

Y

V

F

L

C

I

V

S

S

T

T

D
|
D

K

K

A

A

V

A

R

N

P

P

T

V

N

N

V

M

M

M

G

G

T

A

T

S

K
|
K

R

R

M

I

A

M

E

E

L

M

V

F

L

L

Q

M

A

R

L

K

S

S

K

E

L

-

N

-

S

-

G

E

T

I

R

A

F

I

C

S

M

T

V

A

R

R

F

F

G

A

N
|
N

V
|
V

L

A

G

F

S
|
S

S

D

G

G

S

S

V

-

P

-

L

-

F

-

R

-

K

-

Q

R

I

I

A

Q

N

K

G

N

G

Q

P

P

V

I

T

-

V

-

T

-

H

-

P

-

E

-

I

-

T

-

R

R

F

Y

F

F

M

V

T

T

I

P

P

Q

E

E

A

S

S

G

Q

E

L

L

V

C

I

L

Q

M

A

S

G

C

A

I

M

F

G

G

I

E

G

N

G

R

D

D

V

I

F

F

binding nicotinamide-adenine-dinucleotide: G38 (= G290), S39 (= S291), I40 (= I292), D60 (≠ E312), I61 (≠ L313), L89 (≠ M341), D90 (vs. gap), I91 (≠ M342), L112 (≠ A366), S113 (≠ A367), A114 (= A368), K116 (= K370), D159 (= D411), K172 (= K424), N195 (= N450), V196 (= V451), S199 (= S454)

4tqgA Crystal structure of megavirus udp-glcnac 4,6-dehydratase, 5-epimerase mg534 (see paper)
25% identity, 44% coverage: 276:559/644 of query aligns to 1:246/297 of 4tqgA

query
sites
4tqgA

A

S

N

Q

I

I

T

N

G

D

K

K

V

T

V

I

M

M

V

I

T

F

G
|
G

A

G

G
x
S

G
|
G

S
|
S

I
x
L

G

G

S

N

E

R

L

L

C

I

R

E

Q

T

I

Y

L

I

R

N

L

-

A

-

P

N

R

N

K

I

L

I

V

V

L

N

F

Y

E

S

L
x
R

S
x
D

E

E

F

S

G

K

L

H

Y

W

S

S

I

M

E

E

R

L

E

K

L

Y

Q

K

A

S

I

D

R

K

A

-

E

-

L

-

G

-

L

-

D

-

V

-

E

-

L

L

F

K

P

N

M

I

G

G

S
x
D

V
x
I

Q

R

R

D

Q

F

N

E

R

K

V

V

Q

Q

A

Q

V

S

M

I

E

M

A

R

F

I

K

N

V

P

Q

D

T

I

V

I

Y

I

H

I

A
|
A

Y

L

K
|
K

H

H

V

I

P

D

L

R

V

C

E

E

H

Y

N

E

V

I

V

N

E

E

G

C

V

L

R

D

N

T

N

N

V

I

F

K

G

G

-

L

-

Q

-

N

-

V

-

L

T

K

L

V

Y

T

T

E

A

I

Q

N

A

R

A

S

I

N

A

L

T

S

G

N

V

L

E

K

T

A

F

V

V

C

L

F

I
x
V

S

S

T

T

D

D

K

K

A

A

V

C

R

S

P

P

T

V

N

N

V

S

M
x
Y

G

G

T

M

T

S

K
|
K

R

A

M

I

A

C

E

E

L

T

V

L

L

V

Q

V

A

E

L

K

S

S

K

K

L

Y

N

I

S

K

G

D

T

I

R

K

F

Y

C

V

M

C

V

V

R

R

F
x
Y

G
|
G

N

N

V
|
V

L

L

G

N

S

-

G

-

S

-

V

-

P

-

L

-

F

-

R

-

K

-

Q

-

I

-

A

-

N

-

G

-

P

-

V

F

T

T

V

L

T

T

H

H

P

T

E

S

I

M

T

T

R

R

F

F

F

I

M

M

T

T

I

L

P

D

E

D

A

S

S

V

Q

K

L

L

V

I

I

E

Q

Y

A

A

G

I

A

I

M

N

G

G

I

N

G

S

G

G

D

E

V

I

F

V

V

I

L

P

D

K

M

L

G

N

Q

-

S

S

V

M

K

Y

I

I

V

K

D

D

L

-

A

-

R

-

K

-

M

M

I

I

R

E

L

L

S

F

G

A

F

-

E

-

V

-

K

-

D

D

E

K

H

Y

N

-

P

-

D

-

G

-

D

-

I

-

E

P

I

I

Q

V

F

I

S

T

G

G

L

L

R

R

P

S

G

G

E

E

K

R

L

M

Y

Y

E

E

E

S

L

L

L

I

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G12 (= G287), S14 (≠ G289), G15 (= G290), S16 (= S291), L17 (≠ I292), R36 (≠ L313), D37 (≠ S314), D59 (≠ S344), I60 (≠ V345), A81 (= A366), A82 (= A367), A83 (= A368), K85 (= K370), V128 (≠ I408), Y140 (≠ M420), K144 (= K424), Y168 (≠ F448), G169 (= G449), V171 (= V451)

7k3pA The structure of the udp-glc/glcnac 4-epimerase from the human pathogen campylobacter jejuni
29% identity, 27% coverage: 280:454/644 of query aligns to 1:181/329 of 7k3pA

query
sites
7k3pA

G

G

K

S

V

K

V

I

M

L

V

I

T

S

G
|
G

A

G

G

A

G
|
G

S
x
Y

I
|
I

G

G

S

S

E

H

L

T

C

L

R

R

Q

Q

I

F

L

L

R

K

L

T

A

D

P

H

R

E

K

I

L

C

V

V

L

L

F
x
D

E
x
N

L

L

S
|
S

E

K

F
x
G

G

S

L

K

Y

I

S

A

I

I

E

E

R

-

E

D

L

L

Q

A

A

A

I

T

R

R

A

A

E

F

L

K

G

F

L

F

D

E

V

Q

E
x
D

L
|
L

F

S

P

D

M

F

M

Q

G

G

S

-

V

-

Q

-

R

-

Q

-

N

-

R

-

V

V

Q

K

A

A

V

L

M

F

E

E

A

R

F

E

K

K

V

F

Q

D

T

A

V

I

Y

V

H

H

A
x
F

A
|
A

A

A

Y

S

K
x
I

H

E

V

V

P

F

L

E

V

S

E

M

H

Q

N

N

V

P

V

L

E

K

G

Y

V

Y

R

M

N
|
N

N

N

V

T

F

V

G

N

T

T

L

T

Y

N

T

L

A

I

Q

E

A

T

A

C

I

L

A

Q

T

T

G

G

V

V

E

N

T

K

F

F

V

I

L

F

I

S

S

S

T

T

-

A

-

T

-

Y

-

G

-

E

-

P

-

Q

-

T

-

P

-

V

-

S

-

E

D

T

K

S

A

P

V

L

R

A

P

P

T

I

N

N

V

P

M
x
Y

G

G

T

R

T

S

K
|
K

R

L

M

M

A

S

E

E

L

E

V

V

L

L

Q

R

A

D

L

A

S

S

K

M

L

A

N

N

S

P

G

E

T

F

R

K

F

H

C

C

M

I

V

L

R

R

F
x
Y

G

F

N
|
N

V
|
V

L

A

G

G

S

A

binding nicotinamide-adenine-dinucleotide: G8 (= G287), G11 (= G290), Y12 (≠ S291), I13 (= I292), D32 (≠ F311), N33 (≠ E312), S35 (= S314), G37 (≠ F316), D57 (≠ E337), L58 (= L338), F79 (≠ A366), A80 (= A367), I83 (≠ K370), N98 (= N385), Y147 (≠ M420), K151 (= K424), Y175 (≠ F448), N177 (= N450), V178 (= V451)

Q9LPG6 Trifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose-reductase RHM2; NDP-rhamnose synthase; Protein MUCILAGE-MODIFIED 4; Protein RHAMNOSE BIOSYNTHESIS 2; Rhamnose biosynthetic enzyme 2; AtRHM2; UDP-L-rhamnose synthase MUM4; EC 4.2.1.76; EC 1.1.1.-; EC 5.1.3.- from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
23% identity, 42% coverage: 281:550/644 of query aligns to 9:286/667 of Q9LPG6

query
sites
Q9LPG6

K

K

V

N

V

I

M

L

V

I

T

T

G

G

A

A

G

A

G
|
G

S

F

I

I

G

A

S

S

E

H

L

V

C

A

R

N

Q

R

I

L

L

I

R

R

L

N

A

Y

P

P

R

D

-

Y

K
|
K

L

I

V

V

L

V

F

L

E

D

L

K

S

L

E

D

F

Y

G

C

L

-

Y

-

S

-

I

-

E

-

R

S

E

D

L

L

Q

K

A

N

I

L

R

D

A

P

E

S

L

F

G

S

L

S

D

P

V

N

E

F

L

K

F

F

P

-

M

V

M

K

G

G

S

D

V

I

Q

A

R

S

Q

D

N

D

R

L

V

V

Q

N

A

Y

V

L

M

L

E

I

A

T

F

E

K

N

V

I

Q

D

T

T

V

I

Y

M

H

H

A

F

A

A

Y

Q

K

T

H

H

V

V

P
x
D

L

N

V

S

E

F

H

G

N

N

V

S

V

F

E

E

G

F

V

T

R

K

N

N

V

I

F

Y

G

G

T

T

L

H

Y

V

T

L

A

L

Q

E

A

A

A

C

I

K

A

V

T

T

G

G

-

Q

V

I

E

R

T

R

F

F

V

I

L

H

I

V

S

S

T

T

D

D

K

E

-

V

-

Y

-

G

-

E

-

T

-

D

-

E

-

D

-

A

-

V

-

G

-

N

-

H

-

E

-

A

-

S

A

Q

V

L

R

L

P

P

T

T

N

N

V

P

M

Y

G

S

T

A

T

T

K
|
K

R

A

M

G

A

A

E

E

L

M

V

L

L

V

Q

M

A

A

L

Y

S

G

K

R

L

-

N

S

S

Y

G

G

T

L

R

P

F

V

C

I

M

T

V

T

R

R

F

G

G

N

N

N

V

V

L

Y

G
|
G

S

P

S

N

G

Q

-

F

-

P

-

E

S

K

V

M

V

I

P

P

L

K

F

F

R

I

K

L

Q

L

I

A

A

M

N

S

G

G

G

K

P

P

V

L

T

P

V

I

T

-

H

H

P

G

E

D

I

G

T

S

R

N

-

V

-

R

-

S

-

Y

-

L

F

Y

F

C

M

E

T

D

I

V

P

A

E

E

A

A

S

F

Q

E

L

V

V

V

I

L

Q

H

A

K

G

G

A

E

M

I

G

-

I

-

G

-

G

G

D

H

V

V

F

Y

V

N

L

V

D

G

M

T

G

K

Q

R

S

E

V

R

K

R

I

V

V

I

D

D

L

V

A

A

R

R

K

D

M

I

I

C

R

K

L

L

S

F

G

G

F

-

E

-

V

-

K

-

D

-

E

-

H

-

N

-

P

K

D

D

G

P

D

E

I

S

E

S

I

I

Q

Q

F

F

S

V

G

E

L

N

R

R

P

P

G18 (= G290) mutation to A: Abolishes dehydratase activity.
K36 (= K307) mutation to A: Reduces dehydratase activity.
D96 (≠ P373) mutation to N: In mum4-1; no extruded mucilage and seed coat defects. Abolishes dehydratase activity.
K165 (= K424) mutation to A: Abolishes dehydratase activity.
G193 (= G453) mutation to R: In mum4-2; no extruded mucilage and seed coat defects. Abolishes dehydratase activity.

Sites not aligning to the query:

392 G→A: No effect on dehydratase activity.
413 K→A: No effect on dehydratase activity.
518 K→A: No effect on dehydratase activity.

Q9SYM5 Trifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose-reductase RHM1; Protein REPRESSOR OF LRX1 1; Rhamnose biosynthetic enzyme 1; AtRHM1; EC 4.2.1.76; EC 1.1.1.-; EC 5.1.3.- from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
23% identity, 43% coverage: 276:550/644 of query aligns to 2:284/669 of Q9SYM5

query
sites
Q9SYM5

A

A

N

S

I

Y

T

T

G

P

K

K

V

N

V

I

M

L

V

I

T

T

G

G

A

A

G

A

G

G

S

F

I

I

G

A

S

S

E

H

L

V

C

A

R

N

Q

R

I

L

L

I

R

R

L

S

A

Y

P

P

R

D

K

Y

L

K

V

I

L

V

F

V

E

-

L

L

S

D

E

K

F

L

G

D

L

Y

Y

C

S

S

I

N

E

L

R

K

E

N

L

L

Q

N

A

P

I

S

R

K

A

H

E

S

L

P

G

N

L

F

D

K

V

F

E

-

L

-

F

-

P

-

M

V

M

K

G

G

S

D

V

I

Q

A

R

S

Q

A

N

D

R

L

V

V

Q

N

A

H

V

L

M

L

E

I

A

T

F

E

K

G

V

I

Q

D

T

T

V

I

Y

M

H

H

A

F

A

A

Y

Q

K

T

H

H

V

V

P

D

L

N

V

S

E

F

H

G

N

N

V

S

V

F

E

E

G

F

V

T

R

K

N

N

V

I

F

Y

G

G

T

T

L

H

Y

V

T

L

A

L

Q

E

A

A

A

C

I

K

A

V

T

T

G

G

-

Q

V

I

E

R

T

R

F

F

V

I

L

H

I

V

S

S

T

T

D

D

K

E

-

V

-

Y

-

G

-

E

-

T

-

D

-

E

-

D

-

A

-

L

-

V

-

G

-

N

-

H

-

E

-

A

-

S

A

Q

V

L

R

L

P

P

T

T

N

N

V

P

M

Y

G

S

T

A

T

T

K

K

R

A

M

G

A

A

E

E

L

M

V

L

L

V

Q

M

A

A

L

Y

S

G

K

R

L

-

N

S

S

Y

G

G

T

L

R

P

F

V

C

I

M

T

V

T

R

R

F

G

G

N

N

N

V

V

L

Y

G

G

S

P

S

N

G

Q

S

F

V

P

V

E

P

K

L

L

F

I

R

P

K

K

Q

F

I

I

-

L

A

A

N

M

G

R

G

G

P

Q

V

V

T

L

V

P

T

I

H

H

P

G

E

D

I

G

T

S

R

N

-

V

-

R

-

S

-

Y

-

L

F

Y

F

C

M

E

T

D

I

V

P

A

E

E

A

A

S

F

Q

E

L

V

V

V

I

L

Q

H

A

K

G

G

A

E

M

V

G

-

I

-

G

-

G

G

D

H

V

V

F

Y

V

N

L

I

D

G

M

T

G

K

Q

K

S

E

V

R

K

R

I

V

V

N

D

D

L

V

A

A

R

K

K

D

M

I

I

C

R

K

L

L

S

-

G

-

F

-

E

-

V

-

K

-

D

-

E

-

H

F

N

N

P

M

D

D

G

P

D

E

I

A

E

N

I

I

Q

K

F

F

S

V

G

D

L

N

R
|
R

P

P

R283 (= R549) mutation to K: In rol1-2; Abolishes dehydratase activity in vitro (PubMed:16766693). Induces aberrant accumulation of flavonols leading to alterations in plant growth and cell shape formation (PubMed:18567791, PubMed:18757557).

Query Sequence

>6938123 FitnessBrowser__SB2B:6938123
MDFFQFLFSLKRPHKRMVSVAVDSVFLVLSFWFALFVRLDSVEVVLNSQFWLLIALVCPV
SIAAFVKLGLYRAVLRYLGTQAMTAIVLGVIVSTLAMVSFAYFLSVDLPRTVPFIFASFA
LVFVGGARALMRSLVGNGINRRGEPVIIYGAGVSGRQTALALAQSHEYHPFAFVDDDETL
HGTVIQGLHVHSPSIIKKLIKQQKASRVLLAMPSASRARRQQILQTLEPLSVKVMTLPAM
ADLVSGNKLFSDVKEVEIEDLLGRDAVAPRKDLMAANITGKVVMVTGAGGSIGSELCRQI
LRLAPRKLVLFELSEFGLYSIERELQAIRAELGLDVELFPMMGSVQRQNRVQAVMEAFKV
QTVYHAAAYKHVPLVEHNVVEGVRNNVFGTLYTAQAAIATGVETFVLISTDKAVRPTNVM
GTTKRMAELVLQALSKLNSGTRFCMVRFGNVLGSSGSVVPLFRKQIANGGPVTVTHPEIT
RFFMTIPEASQLVIQAGAMGIGGDVFVLDMGQSVKIVDLARKMIRLSGFEVKDEHNPDGD
IEIQFSGLRPGEKLYEELLIGDNVEGTGHERIMTAHEVFLTWEELKPVLDKLDMACHAFD
HESIRELMLKAPTGFNPTDGICDLVWLNRRHVEDRSNVVALKGQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory