SitesBLAST
Comparing 6938260 FitnessBrowser__SB2B:6938260 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P0A9G6 Isocitrate lyase; ICL; Isocitrase; Isocitratase; EC 4.1.3.1 from Escherichia coli (strain K12) (see 3 papers)
81% identity, 99% coverage: 6:438/439 of query aligns to 3:434/434 of P0A9G6
- SGW 91:93 (= SGW 95:97) binding substrate
- D157 (= D161) binding Mg(2+)
- C195 (= C199) active site, Proton acceptor; mutation to A: Large decrease in activity.; mutation to S: Large decrease in activity.
- A219 (= A223) mutation to C: Isocitrate lyase activity is reduced compared to the wild-type.
- R232 (= R236) binding substrate
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
1igwC Crystal structure of the isocitrate lyase from the a219c mutant of escherichia coli (see paper)
80% identity, 95% coverage: 6:421/439 of query aligns to 2:416/416 of 1igwC
- active site: Y88 (= Y93), D107 (= D112), D156 (= D161), E158 (= E163), H183 (= H188), E185 (= E190), C194 (= C199), R231 (= R236), E288 (= E293), K311 (≠ Q316), S318 (= S323), S320 (= S325)
- binding pyruvic acid: S90 (= S95), G91 (= G96), W92 (= W97), D156 (= D161), R231 (= R236), T350 (= T355)
1igwA Crystal structure of the isocitrate lyase from the a219c mutant of escherichia coli (see paper)
76% identity, 95% coverage: 6:421/439 of query aligns to 2:396/396 of 1igwA
- active site: Y88 (= Y93), D107 (= D112), D156 (= D161), E158 (= E163), H183 (= H188), E185 (= E190), C194 (= C199), R227 (= R236), E284 (= E293), K307 (≠ Q316)
- binding pyruvic acid: S90 (= S95), W92 (= W97), D156 (= D161), R227 (= R236), T330 (= T355)
6lrtA Crystal structure of isocitrate lyase (caur_3889) from chloroflexus aurantiacus in complex with isocitrate and manganese ion
67% identity, 99% coverage: 5:438/439 of query aligns to 1:423/423 of 6lrtA
7cmyC Isocitrate lyase from bacillus cereus atcc 14579 in complex with magnessium ion, glyoxylate, and succinate
64% identity, 98% coverage: 7:438/439 of query aligns to 1:417/417 of 7cmyC
P9WKK7 Isocitrate lyase; ICL; Isocitrase; Isocitratase; EC 4.1.3.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
62% identity, 97% coverage: 12:439/439 of query aligns to 11:428/428 of P9WKK7
- SGW 91:93 (= SGW 95:97) binding substrate
- D153 (= D161) binding Mg(2+)
- C191 (= C199) mutation to S: Adopts a conformation almost identical to the wild-type.
- GH 192:193 (= GH 200:201) binding substrate
- R228 (= R236) binding substrate
- NCSPS 313:317 (= NCSPS 321:325) binding substrate
- K334 (≠ Q342) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
- T347 (= T355) binding substrate
6c4aA Crystal structure of 3-nitropropionate modified isocitrate lyase from mycobacterium tuberculosis with pyruvate (see paper)
62% identity, 97% coverage: 12:438/439 of query aligns to 12:428/428 of 6c4aA
- active site: Y90 (= Y93), D109 (= D112), D154 (= D161), E156 (= E163), H181 (= H188), E183 (= E190), C192 (= C199), H194 (= H201), R229 (= R236), E286 (= E293), Q309 (= Q316), S316 (= S323), S318 (= S325)
- binding 3-nitropropanoic acid: Y357 (≠ F364), S358 (≠ N365), R380 (≠ A389)
- binding magnesium ion: A277 (= A284), A280 (= A287), Q309 (= Q316)
- binding pyruvic acid: Y90 (= Y93), S92 (= S95), G93 (= G96), W94 (= W97), D154 (= D161), C192 (= C199), R229 (= R236), W284 (= W291), T348 (= T355)
6xppA Crystal structure of itaconate modified mycobaterium tuberculosis isocitrate lyase (see paper)
62% identity, 97% coverage: 12:438/439 of query aligns to 10:426/426 of 6xppA
- active site: Y88 (= Y93), D107 (= D112), D152 (= D161), E154 (= E163), H179 (= H188), E181 (= E190), C190 (= C199), H192 (= H201), R227 (= R236), E284 (= E293), Q307 (= Q316), S314 (= S323), S316 (= S325)
- binding 2-methylidenebutanedioic acid: W92 (= W97), C190 (= C199), H192 (= H201), R227 (= R236), N312 (= N321), S314 (= S323), S316 (= S325), T346 (= T355)
- binding magnesium ion: A275 (= A284), A278 (= A287), Q307 (= Q316)
7rb1A Isocitrate lyase-1 from mycobacterium tuberculosis covalently modified by 5-descarboxy-5-nitro-d-isocitric acid (see paper)
62% identity, 97% coverage: 12:438/439 of query aligns to 11:427/427 of 7rb1A
- binding dihydroxyacetic acid: Y89 (= Y93), S91 (= S95), W93 (= W97), D153 (= D161), R228 (= R236), T347 (= T355)
- binding (3E)-3-(hydroxyimino)propanoic acid: C191 (= C199), G192 (= G200), H193 (= H201), R228 (= R236), S315 (= S323), S317 (= S325), T347 (= T355)
- binding magnesium ion: A276 (= A284), A279 (= A287), Q308 (= Q316)
6wsiA Intact cis-2,3-epoxysuccinic acid bound to isocitrate lyase-1 from mycobacterium tuberculosis (see paper)
62% identity, 97% coverage: 12:438/439 of query aligns to 11:427/427 of 6wsiA
- active site: Y89 (= Y93), D108 (= D112), D153 (= D161), E155 (= E163), H180 (= H188), E182 (= E190), C191 (= C199), H193 (= H201), R228 (= R236), E285 (= E293), Q308 (= Q316), S315 (= S323), S317 (= S325)
- binding magnesium ion: A276 (= A284), A279 (= A287), Q308 (= Q316)
- binding (2R,3S)-oxirane-2,3-dicarboxylic acid: C191 (= C199), G192 (= G200), H193 (= H201), R228 (= R236), E285 (= E293), N313 (= N321), S315 (= S323), S317 (= S325), T347 (= T355)
6vb9A Covalent adduct of cis-2,3-epoxysuccinic acid with isocitrate lyase-1 from mycobacterium tuberculosis (see paper)
62% identity, 97% coverage: 12:438/439 of query aligns to 11:427/427 of 6vb9A