SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 6938538 Sama_2641 polyamine ABC transporter, ATP-binding protein (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
40% identity, 99% coverage: 1:376/378 of query aligns to 1:372/378 of P69874

query
sites
P69874

M

M

A

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M

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N

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L

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C26 (≠ L29) mutation to A: Lower ATPase activity and transport efficiency.
F27 (= F30) mutation to L: Lower ATPase activity and transport efficiency.
F45 (≠ I48) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ S57) mutation to T: Loss of ATPase activity and transport.
L60 (= L63) mutation to F: Lower ATPase activity and transport efficiency.
L76 (= L79) mutation to P: Lower ATPase activity and transport efficiency.
V135 (= V138) mutation to M: Loss of ATPase activity and transport.
D172 (= D175) mutation to N: Loss of ATPase activity and transport.
C276 (≠ P279) mutation to A: Lower ATPase activity and transport efficiency.
E297 (= E302) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
42% identity, 84% coverage: 21:337/378 of query aligns to 7:332/375 of 2d62A

query
sites
2d62A

L

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binding pyrophosphate 2-: P40 (≠ G54), S41 (= S55), G42 (= G56), C43 (≠ S57), G44 (= G58), K45 (= K59), T46 (≠ S60), T47 (= T61)

1g291 Malk (see paper)
42% identity, 80% coverage: 26:329/378 of query aligns to 9:321/372 of 1g291

query
sites
1g291

V

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D

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binding magnesium ion: D69 (= D86), E71 (vs. gap), K72 (vs. gap), K79 (≠ Y90), D80 (≠ E91), Y228 (= Y239), D229 (≠ E240), E292 (= E302), D293 (vs. gap)
binding pyrophosphate 2-: P37 (≠ G54), S38 (= S55), G39 (= G56), C40 (≠ S57), G41 (= G58), K42 (= K59), T43 (≠ S60), T44 (= T61)

Sites not aligning to the query:

binding magnesium ion: 341, 342, 359

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
42% identity, 85% coverage: 19:341/378 of query aligns to 5:314/353 of 1vciA

query
sites
1vciA

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K

D

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A

I

V

M

M

S

N

D

R

G

G

W

Q

I

L

A

L

Q

Q

V

I

G

G

S

S

P

P

M

T

D

E

I

V

Y

Y

E

L

S

R

P

P

N

N

S

S

R

V

M

F

I

V

A

A

E

T

F

F

I

I

G

G

T

A

-

P

-

E

V

M

N

N

L

I

F

L

D

E

C

V

E

S

I

V

I

G

E

D

D

G

E

Y

A

L

D

E

H

G

A

R

I

G

L

F

K

R

S

I

P

-

T

E

L

L

P

P

Q

Q

P

M

F

D

L

L

I

L

G

K

H

D

G

Y

V

V

T

-

S

-

L

-

E

-

D

G

K

K

H

T

V

V

W

L

L

F

A

G

V

I

R

R

P

P

E

E

K

H

T

M

L

T

I

V

T

-

R

-

E

-

Q

-

P

-

E

E

G

G

E

V

Y

H

-

M

-

K

N

R

W

T

A

A

R

R

-

L

-

I

G

G

K

K

V

V

H

D

D

F

I

V

A

E

Y

A

L

L

G

G

G

T

L

D

S

T

V

I

Y

L

Y

H

I

V

K

K

L

F

E

G

D

D

D

E

binding adenosine-5'-triphosphate: F16 (= F30), F19 (≠ V33), P40 (≠ G54), S41 (= S55), G42 (= G56), C43 (≠ S57), G44 (= G58), K45 (= K59), T46 (≠ S60), T47 (= T61)

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
47% identity, 63% coverage: 23:261/378 of query aligns to 6:247/393 of P9WQI3

query
sites
P9WQI3

I

L

E

D

R

H

V

V

S

N

K

K

L

S

F

Y

D

P

D

D

V

G

R

H

-

T

A

A

V

V

D

R

D

D

V

L

S

N

L

L

T

T

I

I

N

A

K

D

G

G

E

E

I

F

F

L

A

I

L

L

L

V

G

G

G

P

S

S

G

G

S

C

G

G

K

K

S

T

T

T

L

T

L

L

R

N

M

M

L

I

A

A

G

G

F

L

E

E

R

D

P

I

T

S

E

S

G

G

R

E

I

L

Y

R

L

I

D

A

G

G

Q

E

D

R

I

V

T

N

D

E

L

K

P

A

P

P

Y

K

E

D

R

R

P

D

I

I

N

A

M

M

M

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

T

T

V

V

E

R

Q

Q

N

N

I

I

A

A

F

F

G

P

L

L

K

T

Q

L

D

A

K

K

M

M

S

R

K

K

A

A

D

D

I

I

S

A

Q

Q

R

K

V

V

Q

S

E

E

M

T

L

A

K

K

L

I

V

L

H

D

M

L

E

T

Q

N

Y

L

A

L

K

D

R

R

K

K

P

P

H

S

Q

Q

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

S

A

L

I

A

V

K

R

R

H

P

P

K

K

L

A

L

F

L

L

L

M

D

D

E

E

P

P

M

L

G

S

A

N

L

L

D

D

K

A

K

K

L

L

R

R

T

V

Q

Q

M

M

Q

R

L

G

E

E

V

I

V

A

E

Q

I

L

L

Q

E

R

R

R

V

L

G

G

V

T

T

T

C

T

V

V

M

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

T

E

E

A

A

M

M

T

T

M

L

A

G

G

D

R

R

I

V

A

V

I

V

M

M

S

Y

D

G

G

G

W

I

I

A

A

Q

Q

Q

V

I

G

G

S

T

P

P

M

E

D

E

I

L

Y

Y

E

E

S

R

P

P

N

A

S

N

R

L

M

F

I

V

A

A

E

G

F

F

I

I

G

G

T

S

-

P

-

A

V

M

N

N

L

F

F

F

D

P

C

A

E

R

I

L

H193 (= H209) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
40% identity, 75% coverage: 21:305/378 of query aligns to 4:289/369 of P19566

query
sites
P19566

L

V

K

Q

I

L

E

R

R

N

V

V

S

T

K

K

L

A

F

W

D

G

D

D

V

V

R

V

A

V

V

S

D

K

D

D

V

I

S

N

L

L

T

D

I

I

N

H

K

D

G

G

E

E

I

F

F

V

A

V

L

F

L

V

G

G

G

P

S

S

G

G

S

C

G

G

K

K

S

S

T

T

L

L

R

R

M

M

L

I

A

A

G

G

F

L

E

E

R

T

P

I

T

T

E

S

G

G

R

D

I

L

Y

F

L

I

D

G

G

E

Q

T

D

R

I

M

T

N

D

D

L

I

P

P

Y

A

E

E

R

R

P

G

I

V

N

G

M

M

M

V

F

F

Q

Q

S

S

Y

Y

A

A

L
|
L

F

Y

P

P

H

H

M

L

T

S

V

V

E

A

Q

E

N

N

I

M

A

S

F

F

G

G

L

L

K

K

Q

L

D

A

K

G

M

A

S

K

K

K

A

E

D

V

I

M

S

N

Q

Q

R

R

V

V

Q

N

E

Q

M

V

L

A

K

E

L

V

V

L

H

Q

M

L

E

A

Q

H

Y

L

A

L

K

E

R

R

K

K

P

P

H

K

Q

A

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

S

T

L

L

A

V

K

A

R

E

P

P

K

R

L

V

L

F

L

L

D

D

E

E

P
|
P

M

L

G

S

A

N

L

L

D
|
D

K

A

L

L

R

R

T

V

Q

Q

M

M

Q

R

L

I

E

E

V

I

V

S

E

R

I

L

L

H

E

K

R

R

V

L

G

G

V

R

T

T

C

M

V

I

M

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

L

A

A

G

D

R

K

I

I

A

V

I

V

M

L

S

D

D

A

G

G

W

R

I

V

A

A

Q

Q

V

V

G

G

S

K

P

P

M

L

D

E

I

L

Y

Y

E

H

S

Y

P

P

N

A

S

D

R

R

M

F

I

V

A

A

E

G

F

F

I

I

G

G

T

S

-

P

-

K

V

M

N

N

L

F

F

L

D

P

C

V

E

K

I

V

I

T

E

A

D

T

E

A

A

I

D

E

H

Q

A

V

I

Q

L

V

K

E

S

L

P

P

T

N

L

R

P

Q

Q

Q

P

I

F

W

L

L

I

P

-

V

-

E

G

S

H

R

G

G

V

V

T

Q

T

V

S

G

L

-

E

-

D

-

K

A

H

N

V

M

W

S

L

L

A

G

V

I

R

R

P

P

E

E

K

H

T

L

L

L

L86 (= L103) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P177) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D182) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
42% identity, 67% coverage: 21:274/378 of query aligns to 3:258/374 of 2awnB

query
sites
2awnB

L

V

K

Q

I

L

E

Q

R

N

V

V

S

T

K

K

L

A

F
x
W

D

G

D

E

V

V

R

V

A
x
V

V

S

D

K

D

D

V

I

S

N

L

L

T

D

I

I

N

H

K

E

G

G

E

E

I

F

F

V

A

V

L

F

L

V

G

G

G

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

M

M

L

I

A

A

G

G

F

L

E

E

R

T

P

I

T

T

E

S

G

G

R

D

I

L

Y

F

L

I

D

G

G

E

Q

K

D

R

I

M

T

N

D

D

L

T

P

P

Y

A

E

E

R

R

P

G

I

V

N

G

M

M

M

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

E

A

Q

E

N

N

I

M

A

S

F

F

G

G

L

L

K

K

Q

L

D

A

K

G

M

A

S

K

K

K

A

E

D

V

I

I

S

N

Q

Q

R

R

V

V

Q

N

E

Q

M

V

L

A

K

E

L

V

V

L

H

Q

M

L

E

A

Q

H

Y

L

A

L

K

D

R

R

K

K

P

P

H

K

Q

A

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

S

T

L

L

A

V

K

A

R

E

P

P

K

S

L

V

L

F

L

L

D

D

E

E

P

P

M

L

G

S

A

N

L

L

D

D

K

A

L

L

R

R

T

V

Q

Q

M

M

Q

R

L

I

E

E

V

I

V

S

E

R

I

L

L

H

E

K

R

R

V

L

G

G

V

R

T

T

C

M

V

I

M

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

L

A

A

G

D

R

K

I

I

A

V

I

V

M

L

S

D

D

A

G

G

W

R

I

V

A

A

Q

Q

V

V

G

G

S

K

P

P

M

L

D

E

I

L

Y

Y

E

H

S

Y

P

P

N

A

S

D

R

R

M

F

I

V

A

A

E

G

F

F

I

I

G

G

T

S

-

P

-

K

V

M

N

N

L

F

F

L

D

P

C

V

E

K

I

V

I

T

E

A

D

T

E

A

A

I

D

D

H

Q

A

V

I

Q

L

V

K

E

S

L

P

P

binding adenosine-5'-diphosphate: W12 (≠ F30), V17 (≠ A35), S37 (= S55), G38 (= G56), C39 (≠ S57), G40 (= G58), K41 (= K59), S42 (= S60), T43 (= T61)

2awoA Crystal structure of the adp-mg-bound e. Coli malk (crystallized with adp-mg) (see paper)
42% identity, 67% coverage: 21:274/378 of query aligns to 3:258/372 of 2awoA

query
sites
2awoA

L

V

K

Q

I

L

E

Q

R

N

V

V

S

T

K

K

L

A

F
x
W

D

G

D

E

V

V

R

V

A
x
V

V

S

D

K

D

D

V

I

S

N

L

L

T

D

I

I

N

H

K

E

G

G

E

E

I

F

F

V

A

V

L

F

L

V

G

G

G

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

M

M

L

I

A

A

G

G

F

L

E

E

R

T

P

I

T

T

E

S

G

G

R

D

I

L

Y

F

L

I

D

G

G

E

Q

K

D

R

I

M

T

N

D

D

L

T

P

P

Y

A

E

E

R

R

P

G

I

V

N

G

M

M

M

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

E

A

Q

E

N

N

I

M

A

S

F

F

G

G

L

L

K

K

Q

L

D

A

K

G

M

A

S

K

K

K

A

E

D

V

I

I

S

N

Q

Q

R

R

V

V

Q

N

E

Q

M

V

L

A

K

E

L

V

V

L

H

Q

M

L

E

A

Q

H

Y

L

A

L

K

D

R

R

K

K

P

P

H

K

Q

A

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

S

T

L

L

A

V

K

A

R

E

P

P

K

S

L

V

L

F

L

L

D
|
D

E

E

P

P

M

L

G

S

A

N

L

L

D

D

K

A

L

L

R

R

T

V

Q

Q

M

M

Q

R

L

I

E

E

V

I

V

S

E

R

I

L

L

H

E

K

R

R

V

L

G

G

V

R

T

T

C

M

V

I

M

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

L

A

A

G

D

R

K

I

I

A

V

I

V

M

L

S

D

D

A

G

G

W

R

I

V

A

A

Q

Q

V

V

G

G

S

K

P

P

M

L

D

E

I

L

Y

Y

E

H

S

Y

P

P

N

A

S

D

R

R

M

F

I

V

A

A

E

G

F

F

I

I

G

G

T

S

-

P

-

K

V

M

N

N

L

F

F

L

D

P

C

V

E

K

I

V

I

T

E

A

D

T

E

A

A

I

D

D

H

Q

A

V

I

Q

L

V

K

E

S

L

P

P

binding adenosine-5'-diphosphate: W12 (≠ F30), V17 (≠ A35), S37 (= S55), G38 (= G56), C39 (≠ S57), G40 (= G58), K41 (= K59), S42 (= S60), T43 (= T61)
binding magnesium ion: S42 (= S60), D157 (= D175)

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
42% identity, 67% coverage: 21:274/378 of query aligns to 3:258/371 of 3puyA

query
sites
3puyA

L

V

K

Q

I

L

E

Q

R

N

V

V

S

T

K

K

L

A

F
x
W

D

G

D

E

V

V

R

V

A
x
V

V

S

D

K

D

D

V

I

S

N

L

L

T

D

I

I

N

H

K

E

G

G

E

E

I

F

F

V

A

V

L

F

L

V

G

G

G
x
P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

M

M

L

I

A

A

G

G

F

L

E

E

R

T

P

I

T

T

E

S

G

G

R

D

I

L

Y

F

L

I

D

G

G

E

Q

K

D

R

I

M

T

N

D

D

L

T

P

P

Y

A

E

E

R

R

P

G

I

V

N

G

M

M

M

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

E

A

Q

E

N

N

I

M

A

S

F

F

G

G

L

L

K

K

Q

L

D

A

K

G

M

A

S

K

K

K

A

E

D

V

I

I

S

N

Q

Q

R

R

V

V

Q

N

E

Q

M

V

L

A

K

E

L

V

V

L

H

Q

M

L

E

A

Q

H

Y

L

A

L

K

D

R
|
R

K

K

P

P

H

K

Q
x
A

L

L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

S

T

L

L

A

V

K

A

R

E

P

P

K

S

L

V

L

F

L

L

D
|
D

E
|
E

P

P

M

L

G

S

A

N

L

L

D

D

K

A

L

L

R

R

T

V

Q

Q

M

M

Q

R

L

I

E

E

V

I

V

S

E

R

I

L

L

H

E

K

R

R

V

L

G

G

V

R

T

T

C

M

V

I

M

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

L

A

A

G

D

R

K

I

I

A

V

I

V

M

L

S

D

D

A

G

G

W

R

I

V

A

A

Q

Q

V

V

G

G

S

K

P

P

M

L

D

E

I

L

Y

Y

E

H

S

Y

P

P

N

A

S

D

R

R

M

F

I

V

A

A

E

G

F

F

I

I

G

G

T

S

-

P

-

K

V

M

N

N

L

F

F

L

D

P

C

V

E

K

I

V

I

T

E

A

D

T

E

A

A

I

D

D

H

Q

A

V

I

Q

L

V

K

E

S

L

P

P

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F30), V17 (≠ A35), P36 (≠ G54), S37 (= S55), G38 (= G56), C39 (≠ S57), G40 (= G58), K41 (= K59), S42 (= S60), T43 (= T61), Q81 (= Q99), R128 (= R146), A132 (≠ Q150), S134 (= S152), G135 (= G153), G136 (= G154), Q137 (= Q155), E158 (= E176), H191 (= H209)
binding magnesium ion: S42 (= S60), Q81 (= Q99), D157 (= D175)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
42% identity, 67% coverage: 21:274/378 of query aligns to 3:258/371 of 3puxA

query
sites
3puxA

L

V

K

Q

I

L

E

Q

R

N

V

V

S

T

K

K

L

A

F
x
W

D

G

D

E

V

V

R

V

A
x
V

V

S

D

K

D

D

V

I

S

N

L

L

T

D

I

I

N

H

K

E

G

G

E

E

I

F

F

V

A

V

L

F

L

V

G

G

G

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

M

M

L

I

A

A

G

G

F

L

E

E

R

T

P

I

T

T

E

S

G

G

R

D

I

L

Y

F

L

I

D

G

G

E

Q

K

D

R

I

M

T

N

D

D

L

T

P

P

Y

A

E

E

R

R

P

G

I

V

N

G

M

M

M

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

E

A

Q

E

N

N

I

M

A

S

F

F

G

G

L

L

K

K

Q

L

D

A

K

G

M

A

S

K

K

K

A

E

D

V

I

I

S

N

Q

Q

R

R

V

V

Q

N

E

Q

M

V

L

A

K

E

L

V

V

L

H

Q

M

L

E

A

Q

H

Y

L

A

L

K

D

R
|
R

K

K

P

P

H

K

Q
x
A

L
|
L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

S

T

L

L

A

V

K

A

R

E

P

P

K

S

L

V

L

F

L

L

D
|
D

E
|
E

P

P

M

L

G

S

A

N

L

L

D

D

K

A

L

L

R

R

T

V

Q

Q

M

M

Q

R

L

I

E

E

V

I

V

S

E

R

I

L

L

H

E

K

R

R

V

L

G

G

V

R

T

T

C

M

V

I

M

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

L

A

A

G

D

R

K

I

I

A

V

I

V

M

L

S

D

D

A

G

G

W

R

I

V

A

A

Q

Q

V

V

G

G

S

K

P

P

M

L

D

E

I

L

Y

Y

E

H

S

Y

P

P

N

A

S

D

R

R

M

F

I

V

A

A

E

G

F

F

I

I

G

G

T

S

-

P

-

K

V

M

N

N

L

F

F

L

D

P

C

V

E

K

I

V

I

T

E

A

D

T

E

A

A

I

D

D

H

Q

A

V

I

Q

L

V

K

E

S

L

P

P

binding adenosine-5'-diphosphate: W12 (≠ F30), V17 (≠ A35), S37 (= S55), G38 (= G56), C39 (≠ S57), G40 (= G58), K41 (= K59), S42 (= S60), T43 (= T61), R128 (= R146), A132 (≠ Q150), L133 (= L151), S134 (= S152), Q137 (= Q155)
binding beryllium trifluoride ion: S37 (= S55), G38 (= G56), K41 (= K59), Q81 (= Q99), S134 (= S152), G135 (= G153), G136 (= G154), E158 (= E176), H191 (= H209)
binding magnesium ion: S42 (= S60), Q81 (= Q99), D157 (= D175)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
42% identity, 67% coverage: 21:274/378 of query aligns to 3:258/371 of 3puwA

query
sites
3puwA

L

V

K

Q

I

L

E

Q

R

N

V

V

S

T

K

K

L

A

F
x
W

D

G

D

E

V

V

R

V

A
x
V

V

S

D

K

D

D

V

I

S

N

L

L

T

D

I

I

N

H

K

E

G

G

E

E

I

F

F

V

A

V

L

F

L

V

G

G

G

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

M

M

L

I

A

A

G

G

F

L

E

E

R

T

P

I

T

T

E

S

G

G

R

D

I

L

Y

F

L

I

D

G

G

E

Q

K

D

R

I

M

T

N

D

D

L

T

P

P

Y

A

E

E

R

R

P

G

I

V

N

G

M

M

M

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

E

A

Q

E

N

N

I

M

A

S

F

F

G

G

L

L

K

K

Q

L

D

A

K

G

M

A

S

K

K

K

A

E

D

V

I

I

S

N

Q

Q

R

R

V

V

Q

N

E

Q

M

V

L

A

K

E

L

V

V

L

H

Q

M

L

E

A

Q

H

Y

L

A

L

K

D

R
|
R

K

K

P

P

H

K

Q
x
A

L

L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

S

T

L

L

A

V

K

A

R

E

P

P

K

S

L

V

L

F

L

L

D

D

E
|
E

P

P

M

L

G

S

A
x
N

L

L

D

D

K

A

L

L

R

R

T

V

Q

Q

M

M

Q

R

L

I

E

E

V

I

V

S

E

R

I

L

L

H

E

K

R

R

V

L

G

G

V

R

T

T

C

M

V

I

M

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

L

A

A

G

D

R

K

I

I

A

V

I

V

M

L

S

D

D

A

G

G

W

R

I

V

A

A

Q

Q

V

V

G

G

S

K

P

P

M

L

D

E

I

L

Y

Y

E

H

S

Y

P

P

N

A

S

D

R

R

M

F

I

V

A

A

E

G

F

F

I

I

G

G

T

S

-

P

-

K

V

M

N

N

L

F

F

L

D

P

C

V

E

K

I

V

I

T

E

A

D

T

E

A

A

I

D

D

H

Q

A

V

I

Q

L

V

K

E

S

L

P

P

binding adenosine-5'-diphosphate: W12 (≠ F30), V17 (≠ A35), S37 (= S55), G38 (= G56), C39 (≠ S57), G40 (= G58), K41 (= K59), S42 (= S60), T43 (= T61), R128 (= R146), A132 (≠ Q150), S134 (= S152), Q137 (= Q155)
binding tetrafluoroaluminate ion: S37 (= S55), G38 (= G56), K41 (= K59), Q81 (= Q99), S134 (= S152), G135 (= G153), G136 (= G154), E158 (= E176), N162 (≠ A180), H191 (= H209)
binding magnesium ion: S42 (= S60), Q81 (= Q99)

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
42% identity, 67% coverage: 21:274/378 of query aligns to 1:256/367 of 1q12A

query
sites
1q12A

L

V

K

Q

I

L

E

Q

R

N

V

V

S

T

K

K

L

A

F
x
W

D

G

D

E

V

V

R

V

A
x
V

V

S

D

K

D

D

V

I

S

N

L

L

T

D

I

I

N

H

K

E

G

G

E

E

I

F

F

V

A

V

L

F

L

V

G

G

G

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

M

M

L

I

A

A

G

G

F

L

E

E

R

T

P

I

T

T

E

S

G

G

R

D

I

L

Y

F

L

I

D

G

G

E

Q

K

D

R

I

M

T

N

D

D

L

T

P

P

Y

A

E

E

R

R

P

G

I

V

N

G

M

M

M

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

E

A

Q

E

N

N

I

M

A

S

F

F

G

G

L

L

K

K

Q

L

D

A

K

G

M

A

S

K

K

K

A

E

D

V

I

I

S

N

Q

Q

R

R

V

V

Q

N

E

Q

M

V

L

A

K

E

L

V

V

L

H

Q

M

L

E

A

Q

H

Y

L

A

L

K

D

R
|
R

K

K

P

P

H
x
K

Q
x
A

L
|
L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

S

T

L

L

A

V

K

A

R

E

P

P

K

S

L

V

L

F

L

L

D

D

E

E

P

P

M

L

G

S

A
x
N

L

L

D

D

K

A

L

L

R

R

T

V

Q

Q

M

M

Q

R

L

I

E

E

V

I

V

S

E

R

I

L

L

H

E

K

R

R

V

L

G

G

V

R

T

T

C

M

V

I

M

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

L

A

A

G

D

R

K

I

I

A

V

I

V

M

L

S

D

D

A

G

G

W

R

I

V

A

A

Q

Q

V

V

G

G

S

K

P

P

M

L

D

E

I

L

Y

Y

E

H

S

Y

P

P

N

A

S

D

R

R

M

F

I

V

A

A

E

G

F

F

I

I

G

G

T

S

-

P

-

K

V

M

N

N

L

F

F

L

D

P

C

V

E

K

I

V

I

T

E

A

D

T

E

A

A

I

D

D

H

Q

A

V

I

Q

L

V

K

E

S

L

P

P

binding adenosine-5'-triphosphate: W10 (≠ F30), V15 (≠ A35), S35 (= S55), G36 (= G56), C37 (≠ S57), G38 (= G58), K39 (= K59), S40 (= S60), T41 (= T61), R126 (= R146), K129 (≠ H149), A130 (≠ Q150), L131 (= L151), S132 (= S152), G133 (= G153), G134 (= G154), Q135 (= Q155), N160 (≠ A180), H189 (= H209)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
42% identity, 67% coverage: 21:274/378 of query aligns to 4:259/371 of P68187

query
sites
P68187

L

V

K

Q

I

L

E

Q

R

N

V

V

S

T

K

K

L

A

F

W

D

G

D

E

V

V

R

V

A

V

V

S

D

K

D

D

V

I

S

N

L

L

T

D

I

I

N

H

K

E

G

G

E

E

I

F

F

V

A

V

L

F

L

V

G

G

G

P

S

S

G

G

S

C

G

G

K

K

S

S

T

T

L

L

R

R

M

M

L

I

A

A

G

G

F

L

E

E

R

T

P

I

T

T

E

S

G

G

R

D

I

L

Y

F

L

I

D

G

G

E

Q

K

D

R

I

M

T

N

D

D

L

T

P

P

Y

A

E

E

R

R

P

G

I

V

N

G

M

M

M

V

F

F

Q

Q

S

S

Y

Y

A
|
A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

E

A

Q

E

N

N

I

M

A

S

F

F

G

G

L

L

K

K

Q

L

D

A

K

G

M

A

S
x
K

K

K

A

E

D

V

I

I

S

N

Q

Q

R

R

V
|
V

Q

N

E

Q

M
x
V

L

A

K
x
E

L

V

V

L

H

Q

M

L

E
x
A

Q

H

Y

L

A

L

K

D

R

R

K

K

P

P

H

K

Q

A

L

L

S

S

G

G

G
|
G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

S

T

L

L

A

V

K

A

R

E

P

P

K

S

L

V

L

F

L

L

D
|
D

E

E

P

P

M

L

G

S

A

N

L

L

D

D

K

A

L

L

R

R

T

V

Q

Q

M

M

Q

R

L

I

E

E

V

I

V

S

E

R

I

L

L

H

E

K

R

R

V

L

G

G

V

R

T

T

C

M

V

I

M

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

L

A

A

G

D

R

K

I

I

A

V

I

V

M

L

S

D

D

A

G

G

W

R

I

V

A

A

Q

Q

V

V

G

G

S

K

P

P

M

L

D

E

I

L

Y

Y

E

H

S

Y

P

P

N

A

S

D

R
|
R

M

F

I

V

A

A

E

G

F

F

I

I

G

G

T

S

-

P

-

K

V

M

N

N

L
x
F

F

L

D

P

C

V

E

K

I

V

I

T

E

A

D

T

E

A

A

I

D

D

H

Q

A

V

I

Q

L

V

K

E

S

L

P

P

A85 (= A102) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ S123) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (= V131) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ M134) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ K136) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ E141) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G154) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D175) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (= R245) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
F241 (≠ L256) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

7cagC Mycobacterium smegmatis lpqy-sugabc complex in the catalytic intermediate state (see paper)
46% identity, 62% coverage: 23:257/378 of query aligns to 5:239/362 of 7cagC

query
sites
7cagC

I

L

E

D

R

R

V

V

S

T

K

K

L

S

F

Y

D

P

D

-

V

-

R
x
A

A

A

V

V

D

K

D

E

V

F

S

S

L

M

T

T

I

I

N

A

K

D

G

G

E

E

I

F

F

I

A

I

L

L

L

V

G

G

G
x
P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

T

L

L

R

N

M

M

L

I

A

A

G

G

F

L

E

E

R

E

P

I

T

T

E

S

G

G

R

E

I

L

Y

R

L

I

D

G

G

G

Q

E

D

R

I

V

T

N

D

E

L

K

P

A

P

P

Y

K

E

D

R

R

P

D

I

I

N

A

M

M

M

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

T

T

V

V

E

R

Q

Q

N

N

I

I

A

A

F

F

G

P

L

L

K

T

Q

L

D

A

K

K

M

V

S

P

K

K

A

A

D

E

I

I

S

A

Q

A

R

K

V

V

Q

E

E

E

M

T

L

A

K

K

L

I

V

L

H

D

M

L

E

S

Q

E

Y
x
L

A

L

K

D

R
|
R

K

K

P

P

H

G

Q
|
Q

L

L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

S

A

L

I

A

V

K

R

R

S

P

P

K

K

L

A

L

F

L

L

L

M

D

D

E

Q

P

P

M

L

G

S

A

N

L

L

D

D

K

A

K

K

L

L

R

R

T

V

Q

Q

M

M

Q

R

L

A

E

E

V

I

V

S

E

R

I

L

L

Q

E

D

R

R

V

L

G

G

V

T

T

T

C

T

V

V

M

Y

V

V

T

T

H

H

D

D

Q

Q

E

T

E

E

A

A

M

M

T

T

M

L

A

G

G

D

R

R

I

V

A

V

I

V

M

M

S

L

D

A

G

G

W

E

I

V

A

Q

Q

Q

V

I

G

G

S

T

P

P

M

D

D

E

I

L

Y

Y

E

S

S

S

P

P

N

A

S

N

R

L

M

F

I

V

A

A

E

G

F

F

I

I

G

G

T

S

-

P

-

A

V

M

N

N

L

F

F

F

binding adenosine-5'-triphosphate: A14 (≠ R34), P34 (≠ G54), S35 (= S55), G36 (= G56), C37 (≠ S57), G38 (= G58), K39 (= K59), S40 (= S60), T41 (= T61), Q79 (= Q99), L123 (≠ Y143), R126 (= R146), Q130 (= Q150), S132 (= S152), G133 (= G153), G134 (= G154), Q135 (= Q155)
binding magnesium ion: S40 (= S60), Q79 (= Q99)

1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
36% identity, 82% coverage: 23:331/378 of query aligns to 6:310/353 of 1oxvD

query
sites
1oxvD

I

V

E

K

R

N

V

V

S

S

K

K

L

V

F
|
F

D

K

-

G

-

K

V
|
V

R

V

A
|
A

V

L

D

D

D

N

V

V

S

N

L

I

T

N

I

I

N

E

K

N

G

G

E

E

I

R

F

F

A

G

L

I

L

L

G

G

G
x
P

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
x
T

T
|
T

L

F

L

M

R

R

M

I

L

I

A

A

G

G

F

L

E

D

R

V

P

P

T

S

E

T

G

G

R

E

I

L

Y

Y

L

F

D

D

G

D

Q

R

D

L

I

V

T

A

D

S

-

N

-

G

-

K

-

L

-

I

L

V

P

P

Y

E

E

D

R

R

P

K

I

I

N

G

M

M

M

V

F

F

Q
|
Q

S

T

Y

W

A

A

L

L

F

Y

P

P

H

N

M

L

T

T

V

A

E

F

Q

E

N

N

I

I

A

A

F

F

G

P

L

L

K

T

Q

N

D

M

K

K

M

M

S

S

K

K

A

E

D

E

I

I

S

R

Q

K

R

R

V

V

Q

E

E

E

M

V

L

A

K

K

L

I

V

L

H

D

M

I

E

H

Q

H

Y

V

A

L

K

N

R

H

K

F

P

P

H

R

Q

E

L

L

S

S

G

G

Q

Q

R

Q

Q

Q

R

R

V

V

A

A

L

L

A

A

R

R

S

A

L

L

A

V

K

K

R

D

P

P

K

S

L

L

D

D

E
|
E

P

P

M

F

G

S

A

N

L

L

D

D

K

A

K

R

L

M

R

R

T

D

Q

S

M

A

Q

R

L

A

E

L

V

V

V

K

E

E

I

V

L

Q

E

S

R

R

V

L

G

G

V

V

T

T

C

L

V

L

M

V

V

V

T

S

H

H

D

D

Q

P

E

A

E

D

A

I

M

F

T

A

M

I

A

A

G

D

R

R

I

V

A

G

I

V

M

L

S

V

D

K

G

G

W

K

I

L

A

V

Q

Q

V

V

G

G

S

K

P

P

M

E

D

D

I

L

Y

Y

E

D

S

N

P

P

N

V

S

S

R

I

M

Q

I

V

A

A

E

S

F

L

I

I

G

G

T

E

V

I

N

N

L

E

F

L

D

E

C

G

E

K

I

V

I

T

E

N

D

E

E

G

A

V

D

V

H

I

A

G

I

S

L

L

K

R

S

F

P

P

T

-

L

-

P

-

Q

-

P

-

F

-

L

-

I

-

G

-

H

-

G

-

V

-

T

-

T

V

S

S

L

V

E

S

D

S

K

D

H

R

V

A

W

I

L

I

A

G

V

I

R

R

P

P

E

E

K

D

T

V

L

K

I

L

T

S

R

K

E

D

Q

V

P

I

E

K

G

D

E

D

Y

-

N

S

W

W

-

I

-

L

-

V

A

G

R

K

G

G

K

K

V

V

H

K

D

V

I

I

A

G

Y

Y

L

Q

G

G

L

L

binding phosphoaminophosphonic acid-adenylate ester: F13 (= F30), V18 (= V33), A20 (= A35), P39 (≠ G54), S40 (= S55), G41 (= G56), A42 (≠ S57), G43 (= G58), K44 (= K59), T45 (≠ S60), T46 (= T61), Q89 (= Q99), E166 (= E176)
binding magnesium ion: T45 (≠ S60), Q89 (= Q99)

1oxvA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
36% identity, 82% coverage: 23:331/378 of query aligns to 6:310/353 of 1oxvA

query
sites
1oxvA

I

V

E

K

R

N

V

V

S

S

K

K

L

V

F
|
F

D

K

-

G

-

K

V
|
V

R

V

A
|
A

V

L

D

D

D

N

V

V

S

N

L

I

T

N

I

I

N

E

K

N

G

G

E

E

I

R

F

F

A

G

L

I

L

L

G

G

G
x
P

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
x
T

T
|
T

L

F

L

M

R

R

M

I

L

I

A

A

G

G

F

L

E

D

R

V

P

P

T

S

E

T

G

G

R

E

I

L

Y

Y

L

F

D

D

G

D

Q

R

D

L

I

V

T

A

D

S

-

N

-

G

-

K

-

L

-

I

L

V

P

P

Y

E

E

D

R

R

P

K

I

I

N

G

M

M

M

V

F

F

Q

Q

S

T

Y

W

A

A

L

L

F

Y

P

P

H

N

M

L

T

T