SitesBLAST
Comparing 6938618 FitnessBrowser__SB2B:6938618 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3a8iA Crystal structure of et-ehred-5-ch3-thf complex (see paper)
70% identity, 99% coverage: 2:361/364 of query aligns to 1:360/363 of 3a8iA
- active site: D97 (= D98)
- binding 5-methyl-5,6,7,8-tetrahydrofolic acid: M51 (= M52), Y84 (= Y85), D97 (= D98), I99 (= I100), V111 (= V112), N113 (= N114), F173 (= F174), Y188 (= Y189), E195 (= E196), R223 (= R224), M232 (= M233), W252 (= W253)
1worA Crystal structure of t-protein of the glycine cleavage system (see paper)
44% identity, 91% coverage: 4:334/364 of query aligns to 3:330/362 of 1worA
1wopA Crystal structure of t-protein of the glycine cleavage system (see paper)
44% identity, 91% coverage: 4:334/364 of query aligns to 3:330/362 of 1wopA
- active site: D96 (= D98)
- binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: M51 (= M52), L55 (≠ D56), Y83 (= Y85), D96 (= D98), V98 (≠ I100), E106 (≠ Q108), L108 (≠ R110), V110 (= V112), N112 (= N114), I137 (≠ R139), E160 (≠ Q164), Y168 (≠ K172), Y169 (≠ P173), K173 (= K177), S174 (≠ Q178), I175 (vs. gap), E180 (≠ S181), T181 (≠ L182), Y188 (= Y189), E195 (= E196), M197 (≠ I198), R227 (= R224), Y236 (≠ M233)
Sites not aligning to the query:
1wooA Crystal structure of t-protein of the glycine cleavage system (see paper)
44% identity, 91% coverage: 4:334/364 of query aligns to 3:330/362 of 1wooA
- active site: D96 (= D98)
- binding (6s)-5,6,7,8-tetrahydrofolate: M51 (= M52), Y83 (= Y85), D96 (= D98), V98 (≠ I100), V110 (= V112), N112 (= N114), Y168 (≠ K172), Y169 (≠ P173), Y188 (= Y189), E195 (= E196), Y236 (≠ M233)
Sites not aligning to the query:
P48728 Aminomethyltransferase, mitochondrial; Glycine cleavage system T protein; GCVT; EC 2.1.2.10 from Homo sapiens (Human) (see 4 papers)
35% identity, 98% coverage: 3:357/364 of query aligns to 34:395/403 of P48728
- D129 (= D98) mutation D->A,N: Loss of aminomethyltransferase activity.
- N145 (= N114) to I: in GCE2; loss of aminomethyltransferase activity; dbSNP:rs386833682
- E232 (= E196) binding substrate
- R261 (= R224) binding substrate
- G269 (= G232) to D: in GCE2; decreased aminomethyltransferase activity; dbSNP:rs121964981
- R320 (≠ L281) to H: in GCE2; loss of aminomethyltransferase activity; dbSNP:rs121964985
Sites not aligning to the query:
1wsvA Crystal structure of human t-protein of glycine cleavage system (see paper)
35% identity, 97% coverage: 4:357/364 of query aligns to 4:364/371 of 1wsvA
- active site: D98 (= D98)
- binding n-[4-({[(6s)-2-amino-4-hydroxy-5-methyl-5,6,7,8-tetrahydropteridin-6-yl]methyl}amino)benzoyl]-l-glutamic acid: M53 (= M52), L85 (≠ Y85), D98 (= D98), L99 (= L99), I100 (= I100), V112 (= V112), N114 (= N114), F173 (= F174), G193 (= G188), Y194 (= Y189), E201 (= E196), R230 (= R224), L239 (≠ M233)
Sites not aligning to the query:
2gagA Heteroteterameric sarcosine: structure of a diflavin metaloenzyme at 1.85 a resolution (see paper)
28% identity, 93% coverage: 2:340/364 of query aligns to 574:936/965 of 2gagA
Sites not aligning to the query:
- active site: 350, 375
- binding flavin mononucleotide: 510, 511, 517, 521, 549, 551
- binding nicotinamide-adenine-dinucleotide: 134, 135, 137, 138, 139, 158, 159, 160, 165, 166, 204, 205, 249, 250, 295, 381, 417, 418, 423, 424, 425, 554
Q46337 Sarcosine oxidase subunit alpha; Sarcosine oxidase subunit A; Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit alpha; Tetrameric sarcosine oxidase subunit alpha; TSOX subunit alpha; EC 1.5.3.24 from Corynebacterium sp. (strain P-1) (see 2 papers)
28% identity, 96% coverage: 2:351/364 of query aligns to 576:952/967 of Q46337
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 139 G→A: Does not affect activity and binding of NAD(+).
Q9UI17 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Homo sapiens (Human) (see 4 papers)
29% identity, 88% coverage: 36:357/364 of query aligns to 521:848/866 of Q9UI17
- A530 (≠ G45) to G: in dbSNP:rs1805073
- S646 (≠ T161) to P: in dbSNP:rs1805074
Sites not aligning to the query:
- 59:60 binding FAD
- 80:81 binding FAD
- 87:95 binding FAD
- 91 modified: Tele-8alpha-FAD histidine
- 109 H → R: in DMGDHD; shows 10 fold lower catalytic efficiency due to lower cofactor saturation and reduced thermal stability; dbSNP:rs121908331
- 219 binding FAD
- 279 S → P: in dbSNP:rs532964
- 397:402 binding FAD
4pabB Crystal structure of the precursor form of rat dmgdh complexed with tetrahydrofolate (see paper)
29% identity, 88% coverage: 36:357/364 of query aligns to 477:804/824 of 4pabB
- active site: E536 (≠ D98)
- binding (6s)-5,6,7,8-tetrahydrofolate: I523 (≠ Y85), E536 (≠ D98), T538 (≠ I100), I550 (≠ V112), F612 (≠ G176), L613 (≠ K177), Y632 (= Y189), E639 (= E196), F680 (≠ M233), Y700 (≠ W253)
Sites not aligning to the query:
- active site: 53, 102, 226, 255
- binding flavin-adenine dinucleotide: 11, 12, 14, 15, 16, 35, 36, 37, 43, 44, 45, 47, 48, 49, 50, 51, 175, 204, 205, 207, 226, 228, 326, 328, 353, 355, 356, 357, 358
Q63342 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Rattus norvegicus (Rat) (see 2 papers)
29% identity, 88% coverage: 36:357/364 of query aligns to 514:841/857 of Q63342
- ELT 573:575 (≠ DLI 98:100) binding (6S)-5,6,7,8-tetrahydrofolate
- Y669 (= Y189) binding (6S)-5,6,7,8-tetrahydrofolate
- ELY 676:678 (≠ EII 196:198) binding (6S)-5,6,7,8-tetrahydrofolate
- Y737 (≠ W253) binding (6S)-5,6,7,8-tetrahydrofolate
Sites not aligning to the query:
- 52:53 binding FAD
- 73:74 binding FAD
- 80:88 binding FAD
- 84 modified: Tele-8alpha-FAD histidine
- 212 binding FAD
- 244 binding FAD
- 390:395 binding FAD
3ad7A Heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with methylthio acetate (see paper)
29% identity, 74% coverage: 2:270/364 of query aligns to 573:858/963 of 3ad7A
Sites not aligning to the query:
- active site: 349, 374
- binding flavin mononucleotide: 509, 510, 516, 520, 548, 550
- binding nicotinamide-adenine-dinucleotide: 134, 136, 137, 138, 157, 158, 159, 165, 204, 248, 249, 294, 380, 416, 422, 423, 424
1vrqA Crystal structure of heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with folinic acid (see paper)
29% identity, 74% coverage: 2:270/364 of query aligns to 573:858/963 of 1vrqA
- active site: D676 (= D98)
- binding N-{[4-({[(6R)-2-amino-5-formyl-4-oxo-1,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)phenyl]carbonyl}-L-glutamic acid: L631 (≠ M52), Y663 (= Y85), G677 (≠ L99), H690 (≠ V112), I774 (≠ T187), F776 (≠ Y189), E783 (= E196), K822 (≠ A231), F824 (≠ M233)
Sites not aligning to the query:
- active site: 349, 374
- binding flavin mononucleotide: 509, 510, 516, 520, 548, 550
- binding nicotinamide-adenine-dinucleotide: 134, 136, 137, 138, 157, 158, 159, 165, 204, 248, 249, 294, 380, 416, 422, 423, 424
Q50LF0 Sarcosine oxidase subunit alpha; Sarcosine oxidase subunit A; Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit alpha; Tetrameric sarcosine oxidase subunit alpha; TSOX subunit alpha; EC 1.5.3.24 from Corynebacterium sp. (strain U-96) (see 2 papers)
29% identity, 74% coverage: 2:270/364 of query aligns to 574:859/965 of Q50LF0